CN103245626A - 中药材虎杖中元素铜含量的原子吸收光谱测定法 - Google Patents

中药材虎杖中元素铜含量的原子吸收光谱测定法 Download PDF

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CN103245626A
CN103245626A CN 201310141986 CN201310141986A CN103245626A CN 103245626 A CN103245626 A CN 103245626A CN 201310141986 CN201310141986 CN 201310141986 CN 201310141986 A CN201310141986 A CN 201310141986A CN 103245626 A CN103245626 A CN 103245626A
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chinese herbal
atomic absorption
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朱琳
张福胜
于丽颖
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Jilin Institute of Chemical Technology
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Jilin Institute of Chemical Technology
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Abstract

本发明公开了一种火焰原子吸收光谱测定中药材虎杖中元素铜含量的测定方法。样品的处理方法是：准确称取样品分次进行消化，消化液及洗涤液一并转移至50mL容量瓶中，用二次去离子水定容待测。按以下火焰原子吸收光谱条件测定样品吸光值：波长为324.8nm；负高压374V；参比电流6mA；实际电流3mA；燃烧器高度10.2h/mm；空气流量1.1L/min；乙炔流量1.1L/min。按回归曲线A=0.0825C+0.0059计算出样品的含量，本发明测定方法操作简单、快速、灵敏度高,能测定其它中药材中元素铜的含量。

Description

中药材虎杖中元素铜含量的原子吸收光谱测定法
技术领域
本发明涉及火焰原子吸收光谱测定中药材中元素铜含量的方法。
背景技术
虎杖，虎杖又称花斑竹、酸筒杆、酸桶笋、酸汤梗、黄地榆等，为多年生灌木状草本。中医用途：根状茎药用，有活血散痰、祛风解毒、消炎止痛、去湿热黄疸、治慢性气管炎、降低血脂等功效；全草可作兽药，治牛鼓胀症、黄蜂胃病；并可制农药，对防止螟虫、蚜虫等有效；根状茎和叶都含鞣质，可提制栲胶。虎杖可治疗烧伤，外用能促使创面迅速愈合，且具有抗绿脓杆菌的作用。也有将虎杖粉用食油调糊，涂于轻症患者创面；对于重症患者用虎杖根浓汁涂布等。元素铜是中药材中常见的重金属污染物，对人体有一定的危害作用，测定其含量具有重要意义。
发明内容
本发明提供一种针对中药材虎杖中元素铜含量的测定方法，包括如下步骤：1、样品处理；2、火焰原子吸收法最佳的工作条件；3、测定标准样品中铜的含量；4、得出在一定浓度范围的标准曲线回归方程；5、测定样品；6、计算中药材虎杖样品中元素铜的含量。
本发明所述的火焰原子吸收光谱方法，由以下步骤组成：
样品的处理：
准确称取样品，分次于小烧杯中，加入硝酸，浸泡过夜，次日在小烧杯上加上表面皿，加入硝酸，置于电热板上加热，使样品消化完全，加入一定量的高氯酸，消化至淡棕色气体冒尽，样液接近2mL左右，取下冷却，用二次去离子水冲洗小烧杯内壁和表面皿，样液及洗涤液一并转移至50mL容量瓶中，用二次去离子水定容，待测。
火焰原子吸收最佳的工作条件：
波长为324.8nm；负高压374V；参比电流6mA；实际电流3mA；燃烧器高度10.2h/mm；空气流量1.1L/min；乙炔流量1.1L/min。
绘出标准曲线得出标准曲线回归方程：
将1000μg/mL的铜标准贮备液稀释配制铜质量浓度：0.0000、0.2500、1.0000、0.5000、2.5000、5.0000μg/mL，按原子吸收光谱测定条件，分别测定标准系列溶液的吸光值，根据测定结果绘出标准曲线并得出回归方程。元素铜含量C(μg/mL)的标准曲线回归方程A(吸光值)为：A=0.0825C+0.0059，R2为0.9943，铜含量的浓度线性范围为0.2500～5.0000μg/mL。
样品的测定：
按火焰原子吸收光谱测定条件测定出中药材虎杖样品的吸光值。
计算中药材样品中元素铜含量：
由测得的中药材虎杖样品的吸光值，根据回归曲线计算出样品元素铜的含量。
附图说明：
图1是吸光度（A）与铜含量（C）关系曲线。
具体实施方式
准确称取样品，分次于小烧杯中，加入硝酸，浸泡过夜，次日在小烧杯上加上表面皿，加入硝酸，置于电热板上加热，使样品消化完全，加入一定量的高氯酸，消化至淡棕色气体冒尽，样液接近2mL左右，取下冷却，用二次去离子水冲洗小烧杯内壁和表面皿，样液及洗涤液一并转移至50mL容量瓶中，用二次去离子水定容，待测。
火焰原子吸收最佳的工作条件：
波长为324.8nm；负高压374V；参比电流6mA；实际电流3mA；燃烧器高度10.2h/mm；空气流量1.1L/min；乙炔流量1.1L/min。
标准曲线回归方程：
元素铜含量C(μg/mL)的标准曲线回归方程A(吸光值)为A=0.0825C+0.0059，R2为0.9943，铜含量的浓度线性范围为0.2500～5.0000μg/mL。
样品的测定：
按火焰原子吸收光谱测定条件测定出中药材虎杖样品的吸光值。
计算中药材虎杖样品中元素铜含量：
由测得的样品吸光值，根据标准曲线回归方程计算出中药材虎杖样品中元素铜的含量。

Claims (2)

1.火焰原子吸收最佳的工作条件：波长为324.8nm；负高压374V；参比电流6mA；实际电流3mA；燃烧器高度10.2h/mm；空气流量1.1L/min；乙炔流量1.1L/min。
2.元素铜含量C(μg/mL)的标准曲线回归方程A(吸光值)为：A=0.0825C+0.0059，R2为0.9943，铜含量的浓度线性范围为0.2500～5.0000μg/mL。
CN 201310141986 2013-04-23 2013-04-23 中药材虎杖中元素铜含量的原子吸收光谱测定法 Pending CN103245626A (zh)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104020123A (zh) * 2014-06-23 2014-09-03 中国水产科学研究院南海水产研究所 一种海蜇中铝的氧化亚氮-乙炔火焰原子吸收检测方法
CN104764704A (zh) * 2015-04-22 2015-07-08 阳谷祥光铜业有限公司 闪速吹炼炉渣中铜物相的测定方法
CN104865211A (zh) * 2015-06-19 2015-08-26 贵州出入境检验检疫局检验检疫综合技术中心 一种高效测定谷物类食品中铜、铁、锰和钙含量的方法

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104020123A (zh) * 2014-06-23 2014-09-03 中国水产科学研究院南海水产研究所 一种海蜇中铝的氧化亚氮-乙炔火焰原子吸收检测方法
CN104764704A (zh) * 2015-04-22 2015-07-08 阳谷祥光铜业有限公司 闪速吹炼炉渣中铜物相的测定方法
CN104764704B (zh) * 2015-04-22 2017-10-24 阳谷祥光铜业有限公司 闪速吹炼炉渣中铜物相的测定方法
CN104865211A (zh) * 2015-06-19 2015-08-26 贵州出入境检验检疫局检验检疫综合技术中心 一种高效测定谷物类食品中铜、铁、锰和钙含量的方法

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Application publication date: 20130814