CN103239609A

CN103239609A - Radix Ophiopogonis-containing traditional Chinese medicinal composition

Info

Publication number: CN103239609A
Application number: CN2012100226564A
Authority: CN
Inventors: 张强; 王磊
Original assignee: Lerentang Pharmaceutical Factory of Tianjin Zhongxin Pharmaceutical Group Co Ltd
Current assignee: Lerentang Pharmaceutical Factory of Tianjin Zhongxin Pharmaceutical Group Co Ltd
Priority date: 2012-02-02
Filing date: 2012-02-02
Publication date: 2013-08-14

Abstract

The invention relates to a Radix Ophiopogonis-containing traditional Chinese medicinal composition, and a preparation method and uses thereof. The composition is prepared through using Rehmannia glutinosa Libosch, Millettia dielsiana, Radix Ophiopogonis, licorice root, Polygonum multiflorum, donkey-hide gelatin, Chinese magnoliavine, Manyprickle Acathopanax Root, Tortoiseshell, Chinese date and cassia twig. The composition has the heart nourishing, blood enriching, blood circulation promoting and pain relieving efficacies, and can be used for treating chest stuffiness and pains, palpitations and the like clinically. The invention also discloses the preparation method of the composition.

Description

A kind of Chinese medicine composition that contains Radix Ophiopogonis

Technical field

The present invention relates to field of medicaments, be specifically related to a kind of Chinese medicine composition that contains Radix Ophiopogonis, and its production and use.

Background technology

Be the liliaceous plant tuber of Radix Ophiopogonis Radix Ophiopogonis, is a kind of conventional Chinese medicine, for Liliaceae Liriope and Ophiopogon plant, with the tuber hyoscine.Has the effect of supporting bright heat clearing away, nourishing the lung to arrest cough.Modern medicine think have heart tonifying Radix Ophiopogonis, diuresis, antibiotic effect.Cure mainly diseases such as consumption of body fluid caused by febrile disease, vexed, thirsty, dry pharynx, lung-heat type cough, hemoptysis of pulmonary tuberculosis, throat pain.

It is fast or slow that " arrhythmia " refers to heart beating, surpassed general range.The tachycardia that abnormality of cardiac automaticity or conductive impairment cause, bradycardia or arrhythmia; Psychentonia, a large amount of smoking, drink, drink that strong tea or coffee, overtired, serious insomnia etc. are normal is ARR risk factor; Arrhythmia is more common in the cardiac especially, also often occur in anesthesia, the operation in or the operation after.Arrhythmia belongs to the cardiopalmus, palpitation with a distress feeling, the thoracic obstruction of the traditional Chinese medical science, category such as dizzy, shows as irregularly intermittent pusle, regularly intermittent pulse, irregular-rapid pulse, retarded pulse and rapid pulse etc. at pulse condition.The traditional Chinese medical science is generally treated with decoction arrhythmia.At first should distinguish the type of disease during diagnosis and treatment, and then select different decoction for the treatment for use according to dissimilar.

1, the deficient type of the motive

Show as palpitation and uneasiness, white, the uncomfortable in chest weak breath of complexion white light, spiritlessness and weakness, pale lips, hands and feet being not warm, the lazy speech of spontaneous perspiration.Pale tongue, white and thin fur, weak pulse is unable.

2, flaccidity of the heart-yang type

Show as shortness of breath and palpitation, weak breath is unable, and low voice breath is short, and painful abdominal mass is vexed in the heart, at nightfall for very.Fear of cold happiness temperature or extreme cold of the limbs, clear urine in large amounts, soft stool.Pale tongue, white and moist fur, deep pulse or deep and slow, late heavy.

3, insufficiency of heart-YIN type

Show as the cardiopalmus agitation, have a dizzy spell, flushed cheeks tinnitus, dry mouth and throat and drink fewly, the difference of sleeping dreaminess, low grade fever night sweat, body constitution are thin and weak.Red tongue, few tongue or light stripping, thready and rapid pulse.

4, type of insufficiency of heart-blood

Show as that cardiopalmus, palpitation with a distress feeling, the head are giddy, lusterless complexion, dysphoria and insomnia, dreaminess, lethargy, lip and nail color be light.Pale tongue, white and thin fur, thready and weak pulse.

5, type of deficiency of both the heart and spleen

Show as palpitation and amnesia, insomnia and dreamful sleep, lusterless complexion, spiritlessness and weakness, inappetence, abdominal distention loose stool.The woman can have that menstrual blood volume is few, color is light or be not to the utmost dripping.Pale and tender tongue, thready and weak pulse.

6, kidney-essence deficiency

(1) congenital defect hypoplasia: be more common in baby's congenital heart disease, show as hypoevolutism, of short and small stature, often out of breath, cardiopalmus is uncomfortable in chest, the lip dark purple, cardiopalmus, sx uncomfortable in chest, out of breath even apasm of coughing occurs, can be alleviated under the quiescent condition when movable.Body constitution is thin and weak, easy to catch cold, indigestion and loss of appetite, irregularly intermittent and regularly intermittent pulse.

(2) throw off restraint the day after tomorrow hack wounds, kidney essense is exhausted: because health preserving is careless, sexual urge is excessive, unscrupulously hacks wounds, damage, exhaustion and geromorphism occurs, deafness and tinnitus, forgetful dimly, body constitution is become thin, fatigue and weak, soreness of waist and knee, impotence and seminal emission, loss of hairs luxated tooth.Symptoms such as severe palpitation, insomnia and dreamful sleep, dizzy dizziness appear then.Deficient and thready pulse unable or the knot generation.

Summary of the invention

The object of the invention is to provide a kind of Chinese medicine composition that contains Radix Ophiopogonis.

Another object of the present invention is to provide the preparation method of this Chinese medicine composition.

Another object of the present invention is to provide the purposes of this Chinese medicine composition.

The Chinese medicine composition that the present invention is a kind of to contain Radix Ophiopogonis by Radix Rehmanniae, Caulis Spatholobi, Radix Ophiopogonis, Radix Glycyrrhizae, Radix Polygoni Multiflori, Colla Corii Asini, Fructus Schisandrae Chinensis, Radix Et Caulis Acanthopanacis Senticosi, Carapax Et Plastrum Testudinis, Fructus Jujubae, Ramulus Cinnamomi etc. ten simply Chinese medicine make.The present invention is directed to coronary artery disease with deficiency of both qi and yin and selected medicines such as Radix Rehmanniae, Radix Ophiopogonis, Caulis Spatholobi, having nourishes heart enriches blood, the effect of coronary circulation-promoting pain-relieving.Clinically can be used for treating diseases such as obstruction of qi in the chest and cardialgia, severe palpitation.

Employed medical material all meet " regulation of Chinese pharmacopoeia comprises:

Radix Rehmanniae: this product is the fresh or dried root of scrophulariaceae rehmannia glutinosa plant Rehmania glutinosa Libosch..Excavate autumn, except removing LU, fibrous root and silt, and using fresh herb; Or Radix Rehmanniae slowly dried by the fire to iron to about eighty per cant do.The former practises title " Radix Rehmanniae ", and the latter practises title " Radix Rehmanniae ".This product should meet the pertinent regulations under first the 82nd page of Radix Rehmanniae item of Chinese Pharmacopoeia version in 2005.

Caulis Spatholobi: this product is the dry rattan of leguminous plant spatholobus suberectus Spatholobus suberectus Dunn.Autumn, two seasons of winter gather, and remove branch and leaf, and section is dried.This product should meet the pertinent regulations under first the 134th page of Caulis Spatholobi item of Chinese Pharmacopoeia version in 2005.

Radix Ophiopogonis: this product is the dried root of liliaceous plant Ophiopogon Radix Ophiopogonis japonicus (Thunb.) Ker-Gawl..Excavate summer, cleans, and is exposed to the sun repeatedly, banks up, and to doing very likely, removes fibrous root, drying.This product should meet the pertinent regulations under Chinese Pharmacopoeia version in 2005 item first one the 106th page Radix Ophiopogonis.

Radix Glycyrrhizae: this product is the dry root and rhizome of glycyrrhizic legume Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. Glycyrrhiza glabra L..Spring, Qiu Erji excavate, and remove fibrous root, dry.This product should meet the pertinent regulations under first the 59th page of Radix Glycyrrhizae item of Chinese Pharmacopoeia version in 2005.

Radix Polygoni Multiflori Preparata: this product is the process of preparing Chinese medicine processed goods of Radix Polygoni Multiflori, should meet the pertinent regulations under first the 123rd page of Radix Polygoni Multiflori Preparata item of Chinese Pharmacopoeia version in 2005.

Radix Polygoni Multiflori is the dried root of the few plant Radix Polygoni Multiflori Polygonum multiflorum Thunb. of section. autumn, two season of winter excavate when leaf is withered, and the two ends of pruning are cleaned, individual big piece, the drying of being cut into.This product should meet the pertinent regulations under first the 122nd page of Radix Polygoni Multiflori item of Chinese Pharmacopoeia version in 2005.

Colla Corii Asini: this product be the dry skin of equine species donkey Equus asinus L. or Cortex Dictamni through decoct, the dense solid gum of carefully making.This product should meet the pertinent regulations under first the 130th page of Colla Corii Asini item of Chinese Pharmacopoeia version in 2005.

Fructus Schisandrae Chinensis: this product is the dry mature fruit of magnoliaceae schisandra Schisandra chinensis (Turcz.) Baill..Practise and claim " Radix Schisandrae Bicoloris ".Pluck during fruit maturation autumn, dry or steam after dry, remove carpopodium and impurity.This product should meet the pertinent regulations under first the 44th page of five tastes subitem of Chinese Pharmacopoeia version in 2005.

Radix Et Caulis Acanthopanacis Senticosi: this product is dry root and rhizome or the stem of Araliaceae Radix Et Caulis Acanthopanacis Senticosi Acanthopanax senticosus (Ru-pr.et Maxim.) Harms.Spring, Qiu Erji gather, cleaning, drying.

Carapax Et Plastrum Testudinis (vinegar system): this product is carapace and the sternite of Testudinidae animal Testudinis Chinemys reevresii (Gray).All can catch the whole year, with autumn, two seasons of winter be many, kill after the seizure, or scald deadly with boiling water, strip carapace and sternite, remove residual meat, dry.This product should meet the pertinent regulations under first the 125th page of Carapax Et Plastrum Testudinis (vinegar system) item of Chinese Pharmacopoeia version in 2005.

Fructus Jujubae: this product is the dry mature fruit of Rhamnaceae plant Fructus Jujubae Ziziphus jujuba Mill..Gather during fruit maturation autumn, dries.This product should meet the pertinent regulations under first the 16th page of Fructus Jujubae item of Chinese Pharmacopoeia version in 2005.

Ramulus Cinnamomi: this product is the dry twig of canella Cortex Cinnamomi Cinnamomum cassia Rresl.Spring, Xia Erji gather, and except defoliation, dry, or section are dried.This product should meet the pertinent regulations under first the 195th page of Ramulus Cinnamomi item of Chinese Pharmacopoeia version in 2005.

A kind of Chinese medicine composition that contains Radix Ophiopogonis of the present invention is made up of the medicine of following weight parts: 50～300 parts of Radix Rehmanniae, 50～300 parts of Caulis Spatholobis, 30～100 parts of Radix Ophiopogonis, 30～100 parts in Radix Glycyrrhizae, 30～100 parts of Radix Polygoni Multiflori, 30～100 parts in Colla Corii Asini, 30～100 parts of Fructus Schisandrae Chinensis, 30～100 parts of Radix Et Caulis Acanthopanacis Senticosis, 20～90 parts of Carapax Et Plastrum Testudiniss, 20～90 parts in Fructus Jujubae, 10～30 parts of Ramulus Cinnamomi.

Preferably, a kind of Chinese medicine composition that contains Radix Ophiopogonis of the present invention is made up of the medicine of following weight parts: 80～200 parts of Radix Rehmanniae, 80～200 parts of Caulis Spatholobis, 40～80 parts of Radix Ophiopogonis, 40～80 parts in Radix Glycyrrhizae, 40～80 parts of Radix Polygoni Multiflori, 40～80 parts in Colla Corii Asini, 40～80 parts of Fructus Schisandrae Chinensis, 40～80 parts of Radix Et Caulis Acanthopanacis Senticosis, 30～70 parts of Carapax Et Plastrum Testudiniss, 30～70 parts in Fructus Jujubae, 15～25 parts of Ramulus Cinnamomi.

Most preferably, a kind of Chinese medicine composition that contains Radix Ophiopogonis of the present invention is made up of the medicine of following weight parts: 100 parts of Radix Rehmanniae, 100 parts of Caulis Spatholobis, 60 parts of Radix Ophiopogonis, 60 parts in Radix Glycyrrhizae, 60 parts of Radix Polygoni Multiflori, 60 parts in Colla Corii Asini, 60 parts of Fructus Schisandrae Chinensis, 60 parts of Radix Et Caulis Acanthopanacis Senticosis, 40 parts of Carapax Et Plastrum Testudiniss, 40 parts in Fructus Jujubae, 20 parts of Ramulus Cinnamomi.

In order to reach better effect, the present invention is a kind of to be contained in the Chinese medicine composition of Radix Ophiopogonis, and Radix Polygoni Multiflori can be selected Radix Polygoni Multiflori Preparata, and Carapax Et Plastrum Testudinis can be selected Carapax et Plastrum Testudinis(processed with vinegar).

A kind of Chinese medicine composition that contains Radix Ophiopogonis of the present invention can adopt following method to prepare active component for crude drug: as one or several use in conjunction preparations in extraction, water extraction, decoction and alcohol sedimentation technique, infusion process, percolation, reflux extraction, continuous backflow extraction method, the macroreticular resin absorbing method.But in order to make each crude drug of this medicine bring into play drug effect better, the present invention preferably adopts following technology to extract active component to raw material, make pharmaceutical formulations with acceptable adjuvant pharmaceutically, but this can not limit protection scope of the present invention.

A kind of Chinese medicine composition that contains Radix Ophiopogonis of the present invention, adopt following step to be prepared from:

A. according to the following weight portion material of getting it filled: 50～300 parts of Radix Rehmanniae, 50～300 parts of Caulis Spatholobis, 30～100 parts of Radix Ophiopogonis, 30～100 parts in Radix Glycyrrhizae, 30～100 parts of Radix Polygoni Multiflori, 30～100 parts in Colla Corii Asini, 30～100 parts of Fructus Schisandrae Chinensis, 30～100 parts of Radix Et Caulis Acanthopanacis Senticosis, 20～90 parts of Carapax Et Plastrum Testudiniss, 20～90 parts in Fructus Jujubae, 10～30 parts of Ramulus Cinnamomi;

B. ten flavor medical materials except Colla Corii Asini decoct with water, and get extracting solution, add ethanol and precipitate, and reclaim ethanol preparation extractum, and Colla Corii Asini is worn into fine powder;

Perhaps, more than ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder, gets Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material elder generation water and decoct, and merges precipitate with ethanol behind the decocting liquid, supernatant concentration becomes extractum, and is standby; Medicinal residues reuse alcohol reflux, extracting solution is condensed into extractum, and is standby; Get Caulis Spatholobi, Radix Polygoni Multiflori, Ramulus Cinnamomi alcohol reflux, extracting solution is crossed polyamide column, first water flushing, reuse aqueous alkali eluting is collected eluent and is regulated pH value with hydrochloric acid, is condensed into extractum and is ground into fine powder with Carapax Et Plastrum Testudinis and Colla Corii Asini and mixes, drying, it is standby to get the hybrid medicine powder;

Perhaps, more than ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder, gets Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material water extraction, merges precipitate with ethanol behind the decocting liquid, supernatant concentration is to becoming extractum, and is standby; Get Caulis Spatholobi, Radix Polygoni Multiflori, Ramulus Cinnamomi supercritical CO ₂Extract is got in extraction, adds an amount of anhydrous alcohol solution, carries out enclose with an amount of β-CD, and drying namely gets clathrate, and is standby; Caulis Spatholobi, Radix Polygoni Multiflori ethanol extraction, extracting solution is crossed the aluminium sesquioxide post, first water flushing, reuse aqueous alkali eluting is collected eluent and is regulated pH value with hydrochloric acid, and be condensed into extractum and mix with donkey-hide gelatin fine powder, clathrate with Carapax Et Plastrum Testudinis, drying, it is standby to get the hybrid medicine powder;

Perhaps, more than ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder; Get Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material water extraction, collect extracting solution, concentrate, cross macroporous resin column, wash with water earlier to colourless, the reuse ethanol elution is collected eluent and is condensed into extractum, and is standby; Caulis Spatholobi, Radix Polygoni Multiflori ethanol extraction, extracting solution is condensed into extractum, and is standby; The Ramulus Cinnamomi supercritical CO ₂Extraction volatile oil adds an amount of anhydrous alcohol solution, carries out enclose with an amount of β-CD, and drying namely gets clathrate, mix with Carapax Et Plastrum Testudinis, donkey-hide gelatin fine powder, extractum, and drying, it is standby to get the hybrid medicine powder;

C. with step b in the arbitrary extractum or the powder mixes that obtain, make preparation.

Preferably, a kind of Chinese medicine composition that contains Radix Ophiopogonis of the present invention, adopt following step to be prepared from:

A. according to the following weight portion material of getting it filled: 80～200 parts of Radix Rehmanniae, 80～200 parts of Caulis Spatholobis, 40～80 parts of Radix Ophiopogonis, 40～80 parts in Radix Glycyrrhizae, 40～80 parts of Radix Polygoni Multiflori, 40～80 parts in Colla Corii Asini, 40～80 parts of Fructus Schisandrae Chinensis, 40～80 parts of Radix Et Caulis Acanthopanacis Senticosis, 30～70 parts of Carapax Et Plastrum Testudiniss, 30～70 parts in Fructus Jujubae, 15～25 parts of Ramulus Cinnamomi;

B. ten flavor medical materials except Colla Corii Asini decoct with water 1～3 time, for the first time be extracted as 2～3 hours, all the other are extracted as 0.5～1 hour, merge extractive liquid,, filter, add ethanol precipitation and make that to contain the alcohol amount be 60～80%, standing over night, it is 1.10～1.30 extractum (60 ℃) that recovery ethanol is condensed into relative density, and Colla Corii Asini is worn into fine powder;

Perhaps, more than ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder, get Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material elder generation water decocts 2～3 times, each 1～3 hour, merge and to add 90% ethanol behind the decocting liquid and precipitate, make to contain the alcohol amount and reach 50～70%, supernatant concentration becomes the extractum of relative density 1.10～1.30 (60 ℃), and is standby; Medicinal residues reuse alcohol reflux 2～3 times, each 1～3 hour, it is standby that extracting solution is condensed into the extractum of relative density 1.10～1.30 (60 ℃); Get Caulis Spatholobi, Radix Polygoni Multiflori, Ramulus Cinnamomi alcohol reflux 2～3 times, each 1～3 hour, extracting solution is crossed polyamide column, elder generation's water flushing is not to there being the alcohol flavor, and reuse aqueous alkali eluting is collected eluent and regulated pH value 8～10 with hydrochloric acid, be condensed into the extractum of relative density 1.10～1.30 (60 ℃), mix with the Colla Corii Asini powder and fine powder with Carapax Et Plastrum Testudinis again, drying, it is standby to get the hybrid medicine powder;

Perhaps, more than ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder, get Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material water extraction 2～3 times, each 1～3 hour, merge and to add 90% ethanol behind the decocting liquid and precipitate, make to contain the alcohol amount and reach 50～70%, supernatant concentration is standby to the extractum that becomes relative density 1.10～1.30 (60 ℃); Get Caulis Spatholobi, Radix Polygoni Multiflori, Ramulus Cinnamomi supercritical CO ₂Extraction, extracting pressure 5-40MPa, temperature 35-60 ℃, CO ₂Flow velocity 20-35L/h, extraction 2-5h gets extract, adds an amount of anhydrous alcohol solution, carries out enclose with an amount of β-CD, drying, it is standby namely to get clathrate; Caulis Spatholobi, Radix Polygoni Multiflori are with 60～80% alcohol reflux 2～3 times, each 1～3 hour, extracting solution is crossed the aluminium sesquioxide post, elder generation's water flushing is not to there being the alcohol flavor, and reuse aqueous alkali eluting is collected eluent and regulated pH value 8～10 with hydrochloric acid, be condensed into the extractum of relative density 1.10～1.30 (60 ℃), mix with Colla Corii Asini powder and fine powder, clathrate with Carapax Et Plastrum Testudinis again, drying, it is standby to get the hybrid medicine powder;

Perhaps, more than ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder; Get Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material water extraction 2～3 times, each 1～3 hour, collect extracting solution, concentrate, cross macroporous resin column, wash with water earlier to colourless, reuse 30～40% ethanol elutions, collecting eluent, to be condensed into the extractum of relative density 1.10～1.30 (60 ℃) standby; Caulis Spatholobi, Radix Polygoni Multiflori be with 50～70% alcohol reflux 2～3 times, and each 1～3 hour, it is standby that extracting solution is condensed into the extractum of relative density 1.10～1.30 (60 ℃); The Ramulus Cinnamomi supercritical CO ₂Extraction, extracting pressure 5-40MPa, temperature 35-60 ℃, CO ₂Flow velocity 20-35L/h, extraction 2-5h adds an amount of anhydrous alcohol solution, carries out enclose with an amount of β-CD, and drying namely gets clathrate, mixes with Carapax Et Plastrum Testudinis, donkey-hide gelatin fine powder, extractum, drying, it is standby to get the hybrid medicine powder;

C. mix with the extractum that obtains among the step b, make preparation.

Most preferably, a kind of Chinese medicine composition that contains Radix Ophiopogonis of the present invention, adopt following step to be prepared from:

A. according to the following weight portion material of getting it filled: 100 parts of Radix Rehmanniae, 100 parts of Caulis Spatholobis, 60 parts of Radix Ophiopogonis, 60 parts in Radix Glycyrrhizae, 60 parts of Radix Polygoni Multiflori, 60 parts in Colla Corii Asini, 60 parts of Fructus Schisandrae Chinensis, 60 parts of Radix Et Caulis Acanthopanacis Senticosis, 40 parts of Carapax Et Plastrum Testudiniss, 40 parts in Fructus Jujubae, 20 parts of Ramulus Cinnamomi;

B. ten flavor medical materials except Colla Corii Asini decoct with water 2 times, for the first time be extracted as 2.5 hours, for the second time be extracted as 1 hour, merge extractive liquid,, filter, add ethanol precipitation and make that to contain the alcohol amount be 70%, standing over night, it is 1.10～1.30 extractum (60 ℃) that recovery ethanol is condensed into relative density, and Colla Corii Asini is worn into fine powder;

Perhaps, more than ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder, get Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material elder generation water decocts 2 times, each 2 hours, merge and to add 90% ethanol behind the decocting liquid and precipitate, make to contain the alcohol amount and reach 60%, supernatant concentration becomes the extractum of relative density 1.20 (60 ℃), and is standby; Medicinal residues reuse alcohol reflux 3 times, each 2 hours, it is standby that extracting solution is condensed into the extractum of relative density 1.20 (60 ℃); Get Caulis Spatholobi, Radix Polygoni Multiflori, Ramulus Cinnamomi alcohol reflux 2 times, each 2 hours, extracting solution is crossed polyamide column, elder generation's water flushing is not to there being the alcohol flavor, and reuse aqueous alkali eluting is collected eluent and regulated pH value 9 with hydrochloric acid, be condensed into the extractum of relative density 1.20 (60 ℃), mix with the Colla Corii Asini powder and fine powder with Carapax Et Plastrum Testudinis again, drying, it is standby to get the hybrid medicine powder;

Perhaps, more than ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder, get Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material water extraction 3 times, each 2 hours, merge and to add 90% ethanol behind the decocting liquid and precipitate, make to contain the alcohol amount and reach 60%, supernatant concentration is standby to the extractum that becomes relative density 1.20 (60 ℃); Get Caulis Spatholobi, Radix Polygoni Multiflori, Ramulus Cinnamomi supercritical CO ₂Extraction, extracting pressure 5-40MPa, temperature 35-60 ℃, CO ₂Flow velocity 20-35L/h, extraction 2-5h gets extract, adds an amount of anhydrous alcohol solution, carries out enclose with an amount of β-CD, drying, it is standby namely to get clathrate; Caulis Spatholobi, Radix Polygoni Multiflori are with 70% alcohol reflux 3 times, each 2 hours, extracting solution is crossed the aluminium sesquioxide post, elder generation's water flushing is not to there being the alcohol flavor, and reuse aqueous alkali eluting is collected eluent and regulated pH value 9 with hydrochloric acid, be condensed into the extractum of relative density 1.20 (60 ℃), mix with Colla Corii Asini powder and fine powder, clathrate with Carapax Et Plastrum Testudinis again, drying, it is standby to get the hybrid medicine powder;

Perhaps, more than ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder; Get Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material water extraction 3 times, each 2 hours, collect extracting solution, concentrate, cross macroporous resin column, wash with water earlier to colourless, reuse 35% ethanol elution, collecting eluent, to be condensed into the extractum of relative density 1.20 (60 ℃) standby; Caulis Spatholobi, Radix Polygoni Multiflori be with 60% alcohol reflux 2 times, and each 2 hours, it is standby that extracting solution is condensed into the extractum of relative density 1.20 (60 ℃); The Ramulus Cinnamomi supercritical CO ₂Extraction, extracting pressure 5-40MPa, temperature 35-60 ℃, CO ₂Flow velocity 20-35L/h, extraction 2-5h adds an amount of anhydrous alcohol solution, carries out enclose with an amount of β-CD, and drying namely gets clathrate, mixes with Carapax Et Plastrum Testudinis, donkey-hide gelatin fine powder, extractum, drying, it is standby to get the hybrid medicine powder;

Wherein, in order to reach better effect, the present invention is a kind of to be contained in the Chinese medicine composition of Radix Ophiopogonis, and Radix Polygoni Multiflori can be selected Radix Polygoni Multiflori Preparata, and Carapax Et Plastrum Testudinis can be selected Carapax et Plastrum Testudinis(processed with vinegar).A kind of Chinese medicine composition that contains Radix Ophiopogonis of the present invention can be made arbitrary pharmaceutically acceptable preparation with suitable pharmaceutics adjuvant, for example tablet, granule, capsule, water preparation, tincture and pill etc., further, pill also comprises drop pill and traditional watered pill etc.Wherein, a kind of Chinese medicine composition that contains Radix Ophiopogonis of the present invention preferably adds excipient and makes watered pill, and the plain ball of the watered pill that obtains can directly use also and can use coated back.

B. ten flavor medical materials except Colla Corii Asini decoct with water 2 times, are extracted as 2.5 hours for the first time, are extracted as 1 hour for the second time, merge extractive liquid, filters, and adds ethanol precipitation and makes that to contain the alcohol amount be 70%, standing over night, it is 1.20 extractum (60 ℃) that recovery ethanol is condensed into relative density, Colla Corii Asini is worn into fine powder;

C. with step b in the arbitrary extractum or the powder mixes that obtain, add excipient, make the watered pill, the bag film-coat.

A kind of preparation method that contains the Chinese medicine composition of Radix Ophiopogonis of the present invention comprises the steps:

Perhaps, more than ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder, gets Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material elder generation water and decoct, and merges precipitate with ethanol behind the decocting liquid, supernatant concentration becomes extractum, and is standby; Medicinal residues reuse alcohol reflux, extracting solution is condensed into extractum, and is standby; Get Caulis Spatholobi, Radix Polygoni Multiflori, Ramulus Cinnamomi alcohol reflux, extracting solution is crossed polyamide column, first water flushing, reuse aqueous alkali eluting is collected eluent and is regulated pH value with hydrochloric acid, is condensed into extractum and is broken into fine powder with Colla Corii Asini and Carapax Et Plastrum Testudinis powder and mixes, drying, it is standby to get the hybrid medicine powder;

Preferably, a kind of preparation method that contains the Chinese medicine composition of Radix Ophiopogonis of the present invention comprises the steps:

Most preferably, a kind of preparation method that contains the Chinese medicine composition of Radix Ophiopogonis of the present invention comprises the steps:

A kind of application of pharmaceutical composition in preparation treatment antiarrhythmic medicament that contains Radix Ophiopogonis of the present invention.

Pharmaceutical composition of the present invention has the effect of supplementing QI and nourishing YIN, coronary circulation-promoting pain-relieving, and clinical deficiency of both QI and YIN disease for coronary heart disease, angina pectoris and arrhythmia, disease are seen chest pain, uncomfortable in chest, cardiopalmus, breathed hard, irregularly intermittent and regularly intermittent pulse.

More than form when producing and to increase or to reduce according to corresponding ratio, can be unit with kilogram or with the ton as large-scale production, small-scale production can be unit with the gram also, and weight can increase or reduce, but the crude drug material weight proportion constant rate between each composition.

A kind of pharmaceutical composition that contains Radix Ophiopogonis of the present invention has reasonable recipe, determined curative effect, and advantage such as have no adverse reaction.

Beneficial effect

In order to understand the present invention better, study at aspect the arrhythmia and blood vessel dilating aspect below by " pharmaceutical composition of the present invention ", its beneficial effect is described.

1 test material

1.1 laboratory animal

The KM mice, male and female half and half are purchased the Fukang biotech inc in Beijing China, the quality certification number: SCXK (capital) 2009-0004.

1.2 given the test agent

The experiment medicine: present composition watered pill is prepared according to embodiment 1,2,3,4 method (calling in the following text: " medicine 1 of the present invention ", " medicine 2 of the present invention ", " medicine 3 of the present invention ", " medicine 4 of the present invention ").Need to prove that for the ease of understanding beneficial effect of the present invention better, the applicant selects the method for embodiment 1～4 to prepare the experiment medicine, but this can not limit protection scope of the present invention.

1.3 medicine and reagent

Lidocaine hydrochloride injection, lot number 1008051, Tianjin gold credit aminoacid company limited.

Chloroform, analytical pure, lot number 20110516, Tianjin Kermel Chemical Reagent Co., Ltd..

The noradrenaline bitartrate injection, lot number 1102021, Wuhan Yuanda Pharmaceutical Group Co.,Ltd.

Dimethyl sulfoxide, analytical pure, lot number 20110701, Tianjin Kermel Chemical Reagent Co., Ltd..

Sodium chloride, potassium chloride, calcium chloride, potassium dihydrogen phosphate, bitter salt, sodium bicarbonate, glucose, above reagent is commercially available analytical pure.

1.4 experimental apparatus

Analytical balance, Sartorius BP211D type, Sartorius AG.

HV-4 in vitro tissue organ constant temperature perfusion system, Chengdu TME Technology Co., Ltd..

2. test method

2.1 chloroform brings out the test of quivering of mice chamber

The KM mice is according to the body weight random packet, the blank group, positive drug lignocaine group (15mg/kg), " medicine 1 of the present invention ", " medicine 2 of the present invention ", " medicine 3 of the present invention ", " medicine 4 of the present invention " dosage group (the 20g crude drug/kg), every group 10, male and female half and half.

The lignocaine group is immediate observation behind the tail vein injection single-dose.All the other each groups are gastric infusion, administration every day 1 time, successive administration 14 days.Behind the last administration 60min mice is dropped in the 500ml beaker of back-off, put a 0.5g rayon balls that contains the 5ml chloroform in the beaker, mouse breathing stops to take out immediately cuts the thoracic cavity open, and a situation arises for the perusal ventricular fibrillation, the chamber of the calculating every group of laboratory animal incidence rate of quivering.Increase the 1ml chloroform behind mice of every test in the cotton balls.Result of the test is carried out Fisher check and X 2 test with SPSS11.5 software.

Incidence rate %=(generation chamber quiver number of animals/this group all animals number) * 100% quivers in the chamber

3. result of the test

3.1 chloroform brings out the test of quivering of mice chamber

3.1.1Fisher inspection statistics result:

The results are shown in Table 1.

Table 1 " medicine of the present invention " causes the influence (Fisher check) of quivering the mice chamber to chloroform

Compare with the blank group, ^*P＜0.01, ^*P＜0.05

As shown in Table 1, the lidocaine injection single-dose of 15mg/kg can reduce chloroform and cause the incidence rate of quivering in the mice chamber, compares with matched group, and utmost point significant difference (P＜0.01) is arranged.20g crude drug/kg " medicine of the present invention " can reduce chloroform and cause the incidence rate of quivering in the mice chamber, compares with matched group, and significant difference (P＜0.05, P＜0.01) is arranged.

3.1.2 X 2 test statistical result:

The results are shown in Table 2.

Table 2 " medicine of the present invention " causes the influence (X 2 test) of quivering the mice chamber to chloroform

Compare with the blank group, ^*P＜0.01, ^*P＜0.05

As shown in Table 2, the lidocaine injection single-dose of 15mg/kg can reduce chloroform and cause the incidence rate of quivering in the mice chamber, compares with matched group, and significant difference (P＜0.01) is arranged.20g crude drug/kg " medicine of the present invention " can reduce chloroform and cause the incidence rate of quivering in the mice chamber, and comparing difference with matched group has statistical significance.

4 conclusions

Result of the test shows, " medicine of the present invention " has and reduce chloroform and bring out quiver incidence rate and suppress the vasoconstrictive pharmacological action of isolated rat thoracic aorta that norepinephrine causes of mice chamber.Prompting " medicine of the present invention " has arrhythmia and blood vessel dilating effect.

The specific embodiment

Further set forth the preparation method of medicine of the present invention by the following examples.

Embodiment 1

A. according to the following weight portion material of getting it filled: Radix Rehmanniae 100g, Caulis Spatholobi 100g, Radix Ophiopogonis 60g, Radix Glycyrrhizae 60g, Radix Polygoni Multiflori Preparata 60g, Colla Corii Asini 60g, Fructus Schisandrae Chinensis 60g, Radix Et Caulis Acanthopanacis Senticosi 60g, Carapax Et Plastrum Testudinis (vinegar system) 40g, Fructus Jujubae 40g, Ramulus Cinnamomi 20g;

B. ten flavor medical materials except Colla Corii Asini decoct with water 2 times, are extracted as 2.5 hours for the first time, are extracted as 1 hour for the second time, merge extractive liquid, filters, and adds ethanol precipitation and makes that to contain the alcohol amount be 70%, standing over night reclaims the extractum (60 ℃) that ethanol is condensed into relative density 1.20;

C. Colla Corii Asini is worn into fine powder, mixes with the extractum that obtains among the step b, adds excipient, makes the watered pill, the bag film-coat.

Embodiment 2

B. above ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder, get Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material elder generation water decocts 2 times, each 2 hours, adding 90% ethanol precipitates after merging decocting liquid, make to contain alcohol amount and reach 60%, supernatant concentration becomes the extractum of relative density 1.20 (60 ℃), and is standby; Medicinal residues reuse alcohol reflux 3 times, each 2 hours, it is standby that extracting solution is condensed into the extractum of relative density 1.20 (60 ℃); Get Caulis Spatholobi, Radix Polygoni Multiflori Preparata, Ramulus Cinnamomi alcohol reflux 2 times, each 2 hours, extracting solution is crossed polyamide column, elder generation's water flushing is not to there being the alcohol flavor, and reuse aqueous alkali eluting is collected eluent and regulated pH value 9 with hydrochloric acid, be condensed into the extractum of relative density 1.20 (60 ℃), mix with the Colla Corii Asini powder and fine powder with Carapax Et Plastrum Testudinis again, drying, it is standby to get the hybrid medicine powder;

C. mix with the extractum that obtains among the step b, add excipient, make the watered pill, the bag film-coat.

Embodiment 3

B. above ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder, get Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material water extraction 3 times, each 2 hours, adding 90% ethanol precipitates after merging decocting liquid, make to contain alcohol amount and reach 60%, supernatant concentration is standby to the extractum that becomes relative density 1.20 (60 ℃); Get Caulis Spatholobi, Radix Polygoni Multiflori Preparata, Ramulus Cinnamomi supercritical CO ₂Extraction, extracting pressure 5MPa, 35 ℃ of temperature, CO ₂Flow velocity 20L/h, extraction 2h gets extract, adds an amount of anhydrous alcohol solution, carries out enclose with an amount of β-CD, drying, it is standby namely to get clathrate; Caulis Spatholobi, Radix Polygoni Multiflori Preparata are with 70% alcohol reflux 3 times, each 2 hours, extracting solution is crossed the aluminium sesquioxide post, elder generation's water flushing is not to there being the alcohol flavor, and reuse aqueous alkali eluting is collected eluent and regulated pH value 9 with hydrochloric acid, be condensed into the extractum of relative density 1.20 (60 ℃), mix with Colla Corii Asini powder and fine powder, clathrate with Carapax Et Plastrum Testudinis again, drying, it is standby to get the hybrid medicine powder;

Embodiment 4

B. above ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder; Get Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material water extraction 3 times, each 2 hours, collect extracting solution, concentrate, cross macroporous resin column, wash with water earlier to colourless, reuse 35% ethanol elution, collecting eluent, to be condensed into the extractum of relative density 1.20 (60 ℃) standby; Caulis Spatholobi, Radix Polygoni Multiflori Preparata be with 60% alcohol reflux 2 times, and each 2 hours, it is standby that extracting solution is condensed into the extractum of relative density 1.20 (60 ℃); The Ramulus Cinnamomi supercritical CO ₂Extraction, extracting pressure 5MPa, 35 ℃ of temperature, CO ₂Flow velocity 20L/h, extraction 2h adds an amount of anhydrous alcohol solution, carries out enclose with an amount of β-CD, and drying namely gets clathrate, mixes with Carapax Et Plastrum Testudinis, donkey-hide gelatin fine powder, extractum, drying, it is standby to get the hybrid medicine powder;

Embodiment 5

A. according to the following weight portion material of getting it filled: Radix Rehmanniae 50g, Caulis Spatholobi 50g, Radix Ophiopogonis 30g, Radix Glycyrrhizae 30g, Radix Polygoni Multiflori Preparata 30g, Colla Corii Asini 30g, Fructus Schisandrae Chinensis 30g, Radix Et Caulis Acanthopanacis Senticosi 30g, Carapax Et Plastrum Testudinis (vinegar system) 20g, Fructus Jujubae 20g, Ramulus Cinnamomi 10g;

Embodiment 6

A. according to the following weight portion material of getting it filled: Radix Rehmanniae 300g, Caulis Spatholobi 300g, Radix Ophiopogonis 100g, Radix Glycyrrhizae 100g, Radix Polygoni Multiflori Preparata 100g, Colla Corii Asini 100g, Fructus Schisandrae Chinensis 100g, Radix Et Caulis Acanthopanacis Senticosi 100g, Carapax Et Plastrum Testudinis (vinegar system) 90g, Fructus Jujubae 90g, Ramulus Cinnamomi 30g;

Embodiment 7

B. ten flavor medical materials except Colla Corii Asini decoct with water 3 times, are extracted as 3 hours for the first time, are extracted as 0.5 hour for the second time, for the third time, merge extractive liquid, filters, and adds ethanol precipitation and makes that to contain the alcohol amount be 60%, standing over night reclaims the extractum (60 ℃) that ethanol is condensed into relative density 1.10;

Embodiment 8

B. ten flavor medical materials except Colla Corii Asini decoct with water 1 time, for the first time are extracted as 3 hours, and merge extractive liquid, filters, and add ethanol precipitation and make that to contain the alcohol amount be 60%, and standing over night reclaims the extractum (60 ℃) that ethanol is condensed into relative density 1.10;

Embodiment 9

A. according to the following weight portion material of getting it filled: Radix Rehmanniae 80g, Caulis Spatholobi 80g, Radix Ophiopogonis 40g, Radix Glycyrrhizae 40g, Radix Polygoni Multiflori Preparata 40g, Colla Corii Asini 40g, Fructus Schisandrae Chinensis 40g, Radix Et Caulis Acanthopanacis Senticosi 40g, Carapax Et Plastrum Testudinis (vinegar system) 30g, Fructus Jujubae 30g, Ramulus Cinnamomi 15g;

Embodiment 10

A. according to the following weight portion material of getting it filled: Radix Rehmanniae 200g, Caulis Spatholobi 200g, Radix Ophiopogonis 80g, Radix Glycyrrhizae 80g, Radix Polygoni Multiflori Preparata 80g, Colla Corii Asini 80g, Fructus Schisandrae Chinensis 80g, Radix Et Caulis Acanthopanacis Senticosi 80g, Carapax Et Plastrum Testudinis (vinegar system) 70g, Fructus Jujubae 70g, Ramulus Cinnamomi 25g;

B. ten flavor medical materials except Colla Corii Asini decoct with water 2 times, are extracted as 2.5 hours for the first time, are extracted as 1 hour for the second time, merge extractive liquid, filters, and adds ethanol precipitation and makes that to contain the alcohol amount be 70%, standing over night reclaims the extractum (60 ℃) that ethanol is condensed into relative density 1.30;

Embodiment 11

Embodiment 12

B. ten flavor medical materials except Colla Corii Asini decoct with water 3 times, are extracted as 3 hours for the first time, are extracted as 0.5 hour for the second time, for the third time, merge extractive liquid, filters, and adds ethanol precipitation and makes that to contain the alcohol amount be 60%, standing over night reclaims the extractum (60 ℃) that ethanol is condensed into relative density 1.30;

Embodiment 13

Embodiment 14

B. above ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder, get Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material elder generation water decocts 3 times, each 1 hour, adding 90% ethanol precipitates after merging decocting liquid, make to contain alcohol amount and reach 50%, supernatant concentration becomes the extractum of relative density 1.10 (60 ℃), and is standby; Medicinal residues reuse alcohol reflux 3 times, each 1 hour, it is standby that extracting solution is condensed into the extractum of relative density 1.10 (60 ℃); Get Caulis Spatholobi, Radix Polygoni Multiflori Preparata, Ramulus Cinnamomi alcohol reflux 2 times, each 1 hour, extracting solution is crossed polyamide column, elder generation's water flushing is not to there being the alcohol flavor, and reuse aqueous alkali eluting is collected eluent and regulated pH value 8 with hydrochloric acid, be condensed into the extractum of relative density 1.10 (60 ℃), mix with the Colla Corii Asini powder and fine powder with Carapax Et Plastrum Testudinis again, drying, it is standby to get the hybrid medicine powder;

C. add an amount of sodium carboxymethyl cellulose, Icing Sugar to above-mentioned drug powder, mixing, granule processed sieves, drying, namely.

Embodiment 15

A. take off the row medical material: Radix Rehmanniae 100g, Caulis Spatholobi 100g, Radix Ophiopogonis 60g, Radix Glycyrrhizae 60g, Radix Polygoni Multiflori Preparata 60g, Colla Corii Asini 60g, Fructus Schisandrae Chinensis 60g, Radix Et Caulis Acanthopanacis Senticosi 60g, Carapax Et Plastrum Testudinis (vinegar system) 40g, Fructus Jujubae 40g, Ramulus Cinnamomi 20g is standby;

B. above ten Chinese medicines simply, it is standby that Carapax Et Plastrum Testudinis and Colla Corii Asini are ground into fine powder, get Radix Rehmanniae, Radix Et Caulis Acanthopanacis Senticosi, Radix Ophiopogonis, Radix Glycyrrhizae, Fructus Jujubae, Fructus Schisandrae Chinensis Six-element medical material water extraction 3 times, each 3 hours, adding 90% ethanol precipitates after merging decocting liquid, make to contain alcohol amount and reach 70%, supernatant concentration is standby to the extractum that becomes relative density 1.30 (60 ℃); Get Caulis Spatholobi, Radix Polygoni Multiflori Preparata, Ramulus Cinnamomi supercritical CO ₂Extraction, extracting pressure 40MPa, 60 ℃ of temperature, CO ₂Flow velocity 35L/h, extraction 5h gets extract, adds an amount of anhydrous alcohol solution, carries out enclose with an amount of β-CD, drying, it is standby namely to get clathrate; Caulis Spatholobi, Radix Polygoni Multiflori Preparata are with 80% alcohol reflux 3 times, each 3 hours, extracting solution is crossed the aluminium sesquioxide post, elder generation's water flushing is not to there being the alcohol flavor, and reuse aqueous alkali eluting is collected eluent and regulated pH value 10 with hydrochloric acid, be condensed into the extractum of relative density 1.30 (60 ℃), mix with Colla Corii Asini powder and fine powder, clathrate with Carapax Et Plastrum Testudinis again, drying, it is standby to get the hybrid medicine powder;

Claims

1. a pharmaceutical composition that contains Radix Ophiopogonis is made up of the medicine of following weight parts: 50～300 parts of Radix Rehmanniae, 50～300 parts of Caulis Spatholobis, 30～100 parts of Radix Ophiopogonis, 30～100 parts in Radix Glycyrrhizae, 30～100 parts of Radix Polygoni Multiflori, 30～100 parts in Colla Corii Asini, 30～100 parts of Fructus Schisandrae Chinensis, 30～100 parts of Radix Et Caulis Acanthopanacis Senticosis, 20～90 parts of Carapax Et Plastrum Testudiniss, 20～90 parts in Fructus Jujubae, 10～30 parts of Ramulus Cinnamomi.

2. the described compositions of claim 1 is made up of the medicine of following weight parts: 80～200 parts of Radix Rehmanniae, 80～200 parts of Caulis Spatholobis, 40～80 parts of Radix Ophiopogonis, 40～80 parts in Radix Glycyrrhizae, 40～80 parts of Radix Polygoni Multiflori, 40～80 parts in Colla Corii Asini, 40～80 parts of Fructus Schisandrae Chinensis, 40～80 parts of Radix Et Caulis Acanthopanacis Senticosis, 30～70 parts of Carapax Et Plastrum Testudiniss, 30～70 parts in Fructus Jujubae, 15～25 parts of Ramulus Cinnamomi.

3. the described compositions of claim 1 is made up of the medicine of following weight parts: 100 parts of Radix Rehmanniae, 100 parts of Caulis Spatholobis, 60 parts of Radix Ophiopogonis, 60 parts in Radix Glycyrrhizae, 60 parts of Radix Polygoni Multiflori, 60 parts in Colla Corii Asini, 60 parts of Fructus Schisandrae Chinensis, 60 parts of Radix Et Caulis Acanthopanacis Senticosis, 40 parts of Carapax Et Plastrum Testudiniss, 40 parts in Fructus Jujubae, 20 parts of Ramulus Cinnamomi.

4. the described arbitrary compositions of claim 1～3 is characterized in that described Radix Polygoni Multiflori is Radix Polygoni Multiflori Preparata, and described Carapax Et Plastrum Testudinis is Carapax et Plastrum Testudinis(processed with vinegar).

5. Chinese medicine composition that contains Radix Ophiopogonis, adopt following step to be prepared from:

6. the described compositions of claim 5, adopt following step to be prepared from:

B. ten flavor medical materials except Colla Corii Asini decoct with water 1～3 time, for the first time be extracted as 2～3 hours, all the other are extracted as 0.5～1 hour, merge extractive liquid,, filter, add ethanol precipitation and make that to contain the alcohol amount be 60～80%, standing over night, reclaim the extractum (60 ℃) that ethanol is condensed into relative density 1.10～1.30, Colla Corii Asini is worn into fine powder;

7. the described compositions of claim 5, adopt following step to be prepared from:

B. ten flavor medical materials except Colla Corii Asini decoct with water 2 times, are extracted as 2.5 hours for the first time, are extracted as 1 hour for the second time, merge extractive liquid, filters, and adds ethanol precipitation and makes that to contain the alcohol amount be 70%, standing over night reclaims the extractum (60 ℃) that ethanol is condensed into relative density 1.20, and Colla Corii Asini is worn into fine powder;

8. preparation method that contains the Chinese medicine composition of Radix Ophiopogonis, formed by following steps:

9. the described method of claim 8, formed by following steps:

10. the described method of claim 8, formed by following steps:

11. the application of the described arbitrary pharmaceutical composition of claim 1～7 in the preparation antiarrhythmic drug.