CN103229778A - Cereal crop disease-preventive bactericide - Google Patents
Cereal crop disease-preventive bactericide Download PDFInfo
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- CN103229778A CN103229778A CN2013101650676A CN201310165067A CN103229778A CN 103229778 A CN103229778 A CN 103229778A CN 2013101650676 A CN2013101650676 A CN 2013101650676A CN 201310165067 A CN201310165067 A CN 201310165067A CN 103229778 A CN103229778 A CN 103229778A
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- hexamethylene biguanide
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Abstract
The invention discloses a cereal crop disease-preventive bactericide. The bactericide comprises active ingredients of polyhexamethyleme biguandine hydrochloride or salt compounds of polyhexamethyleme biguandine hydrochloride. The bactericide can effectively prevent various diseases of cereal crops. Proved by tests, the composition has the characteristics of being low in usage cost, safe to use for plants and environments, and the like.
Description
Technical field
The present invention relates to a kind of active ingredient and comprise the application of poly hexamethylene biguanide on control cereal class disease.The invention belongs to the agriculture plant protection field.This bactericide is different from existing other bactericide.
Technical background
Cereal crop is the important parts of cereal crops, comprising: paddy rice, wheat, barley, corn, Chinese sorghum etc.
Paddy rice, wheat, corn are the main cereal crops of China, and agricultural disease is the key factor of harm cereal crop growth always.The normally used medicament of these diseases is conventional dose such as thiophanate methyl, jinggangmeisu, triazolone, carbendazim, because just beginning large tracts of land the seventies from eighties of last century, promote these medicaments, the long-term use kind of high dose ground continuously or the single chemical bactericide of the mode of action influence problems such as residual, the environmental pollution of medicament and the development of pesticide resistance fungi easily.
Poly hexamethylene biguanide is a kind ofly to be acknowledged as 21 century broad spectrum antimicrobicide the most safely and efficiently by Britain Aveciago company development, colorless and odorless, consumption is low, the wide spectrum low toxicity, and speed of action is fast, foam volume is low, and can the back forming for a long time antibacterial of one deck cation at article surface, and can not cause and generate antibiotic bacterium characteristic, its safety obtains U.S. FDA and EPA approval, it is to the half lethal dose LD50 of little muroid〉5000mg/kg, the nontoxic level in true border.Since poly hexamethylene biguanide as cationic germicide in agriculture field is used, can be adsorbed in the bacterial cell surface rapidly, attack fast and destroy cytoplasma membrane, make endochylema composition seepage, thereby reach best sterilization and disinfection effect, pathogenic bacteria, virus are pulled the grass up by its roots.Find that now poly hexamethylene biguanide has remarkable control efficiency to the cereal crop disease, this application technology is different from existing other bactericide.
Summary of the invention
Purpose of the present invention: for control cereal crop disease provides a kind of bactericide of little poison level, realize the purpose of cereal crop disease green, nuisanceless, low-residual control.Result of the test shows that bactericide of the present invention is obvious to the cereal crop disease-controlling effect, has characteristics such as persticide residue is low, formulation rate is few.
Concrete scheme of the present invention is: a kind of bactericide of preventing and treating the cereal crop disease comprises poly hexamethylene biguanide or its esters compound.
This bactericide also can further comprise the conventional auxiliary agent of agricultural chemicals, and namely this bactericide is made up of active ingredient and the conventional auxiliary agent of agricultural chemicals, as effective composition quality content 5～95%, auxiliary agent mass content 5～95%.The formulation of this bactericide can be that agricultural goes up any one formulation that allows, as aqua, wetting powder, soluble powder, microemulsion, aqueous emulsion, suspending agent, missible oil, water dispersible granules etc.
Needed by production technology and application technology, poly hexamethylene biguanide of the present invention can exist with the form of its salt, but its active ingredient that acts on controlling object still is poly hexamethylene biguanide, and the form of its salt can not influence the control efficiency of active ingredient.Be suitable for poly hexamethylene biguanide salt of the present invention and be a kind of in hexamethylene, poly hexamethylene biguanide nitrate, poly hexamethylene biguanide carbonate, poly hexamethylene biguanide phosphate, poly hexamethylene biguanide sulphate, poly hexamethylene biguanide stearate, the poly hexamethylene biguanide acetate.Preferred hexamethylene, as shown in the formula.
Poly hexamethylene biguanide or its esters compound or above-mentioned bactericide can be applicable to prevent and treat cereal crop disease, particularly rice disease, wheat diseases or maize diseases.The inventor finds, poly hexamethylene biguanide or its salt pair cereal crop disease have good control efficiency, as when poly hexamethylene biguanide extension rate during at 1000-5000 times, harmful (as rice blast to rice fungus diseases, rice sheath blight disease, rice green smut, bakanae disease of rice), paddy rice bacteriosis is (as bacterial blight of rice, bacterial leaf streak of rice, the paddy bacterial brown spot, the paddy bacterial basal stem rot), and the paddy rice virus disease (as stripe disease, rice dwarf virus disease, black streak dwarf, yellow dwarf) all demonstrated very high control efficiency; When the control wheat diseases, to wheat fungal diseases (as wheat wheat scab, wheat powdery mildew, wheat rust, take-all, root rotof flax, wheat sharp eyespot, loose kernel smut, bunt), wheat bacteriosis (as bacterial stripe), and wheat virus disease evil (as yellow stunt of wheat viral disease, wheat rosette stunt, stripe virus disease, black streak dwarf) etc. has demonstrated fine control efficiency; When the control of maize disease, maize diseases such as corn northern leaf blight and southern leaf blight, corn banded sclerotial blight, corn stalk rot disease, corn circle pinta, maize head smut are demonstrated the better prevention effect.These new application prevention and control field, Prevention Technique all are that the inventor obtains by lot of experiments, and are different from existing other bactericide.
This poly hexamethylene biguanide or its esters compound or above-mentioned bactericide can be applicable to cereal crop seed-soaking or dressing, or spray more than the crop growth period root in case the evil of curing the disease.As for bactericide of the present invention, the application mode on control cereal crop disease is according to preventing and treating the disease difference, can select the seed-soaking of cereal crop or seed pelleting or convert the spraying of waterleaf face in the cereal crop whole growth phase.
Bactericide of the present invention can use with the form of single agent or mixture, uses with usual auxiliaries such as other carriers, surfactants usually during use.
Suitable additive and carrier can be solid or liquid, and they are material commonly used in the formulation process normally, mineral matter natural or regeneration for example, solvent, dispersant, wetting agent, adhesive, thickener, adhesive or fertilizer.
The method for optimizing of using bactericide of the present invention comprise with composition of the present invention be used for the plant growth acrial part or as soak seed, Cotton seeds, particularly leaf portion uses.Frequency of utilization and amount of application depend on biology and the weather life condition of pathogene.
Composition of the present invention can use with constant form, perhaps preferably with the configuration technology in normally used additive use, therefore these compounds can be configured in known manner, for example make wetting powder, suspending agent, aqua, microemulsion, soluble powder, missible oil, aqueous emulsion etc.The character that has according to these compositions can be selected as spraying, atomizing according to the purpose that will reach and ambient conditions, broadcasts sowing, bold and vigorous application process of watering etc. and so on.
Poly hexamethylene biguanide of the present invention or its salt are when independent control cereal crop disease, and positive control efficiency can show as the reduction with respect to the better effect of other bactericide, amount of application or application times, and action effect instant effect, persistence are longer.
Bactericidal action characteristics of the present invention mainly show as: 1, toxicity of the present invention is low, belongs to little poison level agricultural chemicals, and is remarkable to the rice disease preventive effect, meets the requirement of the nuisanceless production of rice green; 2, bactericide of the present invention is good to crop safety, preventive effect.Through evidence, bactericide chemical property of the present invention is stable; 3, reduce dosage, thereby reduced cost and the pollution that has alleviated environment.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explaining the present invention, and be not used in restriction the present invention, all any modifications of making within the spirit and principles in the present invention, be equal to and replace and improvement etc., all should be included within protection scope of the present invention.
Percentage all is weight percentage in all prescriptions of following examples.The processing technology of fungicide preparation of the present invention is prior art, can change to some extent according to different situations.
One, formulation prepares embodiment
Embodiment 1:20% hexamethylene aqua
Hexamethylene 20%, lignosulfonates 3.8%, propane diols 2%, xanthans 3%, phenethyl phenol formaldehyde resin polyvinylether 4.6%, water complements to 100%.
Above-mentioned former medicine, auxiliary agent are fully mixed, under high-speed stirred, water is mixed with it, namely make aqua.
Embodiment 2:25% poly hexamethylene biguanide wetting powder
Poly hexamethylene biguanide 25%, calcium lignosulfonate 6%, bentonite 3%, NONIN HS 240 4%, attapulgite complements to 100%.
Above-mentioned each component is fully mixed, after ultrafine crusher is pulverized, make wetting powder.
According to said method, can produce 25% poly hexamethylene biguanide nitrate wetting powder, 25% poly hexamethylene biguanide carbonate wetting powder, 25% poly hexamethylene biguanide phosphate wetting powder, 25% poly hexamethylene biguanide sulphate wetting powder, 25% poly hexamethylene biguanide stearate wetting powder, 25% poly hexamethylene biguanide acetate wetting powder.
Embodiment 3:15% hexamethylene suspending agent
Hexamethylene 15%, sodium lignin sulfonate 7%, xanthans 0.8%, bentonite 3%, Magnesiumaluminumsilicate 1%, ethylene glycol 4%, water complements to 100%.
The ratio of each component such as active component hexamethylene and dispersant, wetting agent, thickener and water in prescription mixed, after sand milling and/or high speed shear, obtain semi-finished product, add water after the analysis and mix to filter and get product.
Embodiment 4:30% hexamethylene aqueous emulsion
Hexamethylene 30%, N-repefral 4%, nonyl phenol benzene oxygen vinethene 3%, 2,6-di-tert-butyl-4-methy phenol 2%, ethylene glycol 3%, polyvinyl alcohol 1%, Sodium Benzoate 1%, silicone defoaming agent 0.8%, water complements to 100%.
In the emulsification still, active component hexamethylene and solvent, auxiliary agent are mixed under mechanical agitation, drop into emulsifier then and stabilizing agent stirs, add water at last, under 100-12000 rev/min rotating speed, stirred 10-30 minute, make it to become the product of even emulsus.
Embodiment 5:12% hexamethylene microemulsion
Hexamethylene 12%, acetone 15%, compound sodium nitrophenolate 0.5%, alkyl polyoxyethylene ether and polyoxyethylene nonylphenol ether 5%, propane diols 1%, urea 2%, water complements to 100%.
With the active component hexamethylene in solvent fully the dissolving after, stir, add emulsifier, builder and antifreeze simultaneously, treat above-mentioned system mixing after, again water is slowly added in the above-mentioned mixed liquor, can be made into the microemulsion of different content after fully stirring.
Embodiment 6:20% poly hexamethylene biguanide missible oil
Poly hexamethylene biguanide 20%, Jatropha curcas oil methyl esters 50%, fatty alcohol-polyoxyethylene ether (AEO-5) 6%, styryl phenol polyvinylether (602#) 4%, calcium dodecyl benzene sulfonate 6.5%, Si Pan-601%, dodecyl pyrrolidone 2.5% mixes and obtains 20% poly hexamethylene biguanide missible oil.
Embodiment 7:18% poly hexamethylene biguanide soluble powder
Poly hexamethylene biguanide 18%, aliphatic alcohol polyethenoxy base ether 2%, alkylsulfonate 3%,, sodium sulphate 0.5%, White Carbon black complements to 100%.
After poly hexamethylene biguanide and various auxiliary agent mixed, make this mixture by mixing technologies such as laggard promoting the circulation of qi stream pulverizing.
Embodiment 8:50% hexamethylene water dispersible granules
Hexamethylene 50%, Negel 4%, dodecyl sodium sulfate 3%, ammonium sulfate 3%, precipitated calcium carbonate complements to 100%.
Active component hexamethylene and auxiliary agent and the filler ratio in prescription is mixed, be ground into wetting powder through air-flow, add a certain amount of water mixings extruder grain again, drying makes the water dispersible granules product after sieving.
Two, Application Example (indoor determination of activity)
(1) the indoor determination of activity of poly hexamethylene biguanide or its salt pair rice disease
1, the indoor determination of activity of fungal diseases such as poly hexamethylene biguanide or its salt pair rice blast, rice sheath blight disease, rice green smut.
Adopt the mycelial growth rate method.Dull and stereotyped central authorities connect a bacterium dish at pastille, and mycelia down.Support when above to the discontented culture dish of blank bacterium colony 2/3, measure colony diameter of each processing for 25 ℃.Each bacterium colony is measured 2 times by the right-angled intersection method, represents the size of bacterium colony with its mean.Calculate medicament to the inhibition of thalli growth rate, the concentration for the treatment of of poly hexamethylene biguanide salt is all in the active ingredient poly hexamethylene biguanide.
The blank colony diameter of mycelial growth inhibition rate %=(-chemicals treatment colony diameter)/(blank colony diameter-bacterium dish diameter) * 100
The mycelial growth inhibition rate of table 1 poly hexamethylene biguanide or its salt pair rice blast
Measurement result (table 1) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair rice blast is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair rice blast have the better prevention effect.
The mycelial growth inhibition rate of table 2 poly hexamethylene biguanide or its salt pair rice sheath blight disease
Measurement result (table 2) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair rice sheath blight disease is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair rice sheath blight disease have the better prevention effect.
The mycelial growth inhibition rate of table 3 poly hexamethylene biguanide or its salt pair rice green smut
Measurement result (table 3) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair rice green smut is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair rice green smut have the better prevention effect.
The mycelial growth inhibition rate of table 4 poly hexamethylene biguanide or its salt pair bakanae disease of rice
Measurement result (table 4) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair bakanae disease of rice is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair bakanae disease of rice have the better prevention effect.
2, the indoor determination of activity of bacterial diseases such as poly hexamethylene biguanide or its salt pair bacterial blight of rice, bacterial leaf streak of rice, paddy bacterial brown spot, paddy bacterial basal stem rot.
With pathogen at NA(peptone 5g, dusty yeast 1g, glucose 10g, agar 18g, distilled water 1L PH7.0-7.2) rules above the solid culture medium, cultivates up to growing single bacterium colony down at 28 degrees centigrade.The single bacterium colony of pathogen is to liquid NA medium on the picking NA solid culture medium, and is standby to exponential phase at 30 degrees centigrade, 180rpm constant temperature shaking table shaken cultivation.Medicament and contrast medicament are configured to the hot peptone 3g of certain density toxic NB(ox respectively, peptone 5g, dusty yeast 1g, grape is by 10g, 1L, PH7.0-7.2) liquid nutrient medium, get 5ml to test tube, add 40 μ L and contain the NA liquid nutrient medium of bacterial blight of rice, bacterial leaf streak of rice, paddy bacterial brown spot, paddy bacterial basal stem rot germ respectively, 30 degrees centigrade, 180rpm constant temperature shaking table shaken cultivation 2 days, the bacterium liquid of each concentration is measured OD value (600nm) at spectrophotometer.And measure the NB liquid nutrient medium OD value of drug concentration in addition, the OD value that medicament itself causes is proofreaied and correct.The computing formula of proofreading and correct OD value and preventive effect is as follows:
Proofread and correct OD value=contain bacterium OD value-aseptic culture medium OD value
Preventive effect %=(proofreaies and correct the toxic medium OD value of back control medium bacterium liquid OD value-proofread and correct)/control medium bacterium liquid OD value * 100 afterwards proofreaied and correct
Measure according to the method described above, the inhibition activity of part target compound sees the following form 4, table 5, table 6, table 7.
Table 5. medicament of the present invention suppresses indoor determination of activity to bacterial blight of rice
Measurement result (table 5) shows that poly hexamethylene biguanide or its salt pair bacterial blight of rice germ silk effect are obvious, show that poly hexamethylene biguanide of the present invention or its salt pair bacterial blight of rice have the better prevention effect.
Table 6. medicament of the present invention suppresses indoor determination of activity to xanthomonas oryzae pv. oryzicola
Measurement result (table 6) shows that the sick inhibition of poly hexamethylene biguanide or its salt pair bacterial leaf streak of rice is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair bacterial leaf streak of rice have the better prevention effect.
Table 7. medicament of the present invention suppresses indoor determination of activity to the paddy bacterial brown patch germ
Measurement result (table 7) shows that poly hexamethylene biguanide or its salt pair paddy bacterial brown spot inhibition are obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair paddy bacterial brown spot have the better prevention effect.
Table 8. medicament of the present invention suppresses indoor determination of activity to paddy bacterial basal stem rot bacterium
Measurement result (table 8) shows that poly hexamethylene biguanide or its salt pair paddy bacterial basal stem rot inhibition are obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair paddy bacterial basal stem rot have the better prevention effect.
3, the indoor determination of activity of disease such as the stripe disease that causes of poly hexamethylene biguanide or its salt pair paddy rice microorganism, rice dwarf virus disease, black streak dwarf, yellow dwarf.
Adopt the Gooding method, choose more than 3 weeks of inoculation, respectively by stripe disease virus, rice stunt disease virus, black streaked dwarf virus of rice virus, yellow stunt of rice virus systemic infection paddy rice upper blade, homogenate in phosphate buffer, double gauze filters, and 1000g is centrifugal, handle through 2 polyethylene glycol, centrifugal again, precipitation suspends with phosphate buffer, obtains the crude extract of each germ respectively.
Whole test is carried out under 4 degrees centigrade, and the absorbance with ultraviolet specrophotometer mensuration 260nm wavelength calculates virus concentration according to formula.
Virus concentration (mg/ml)=(A260* extension rate)/wherein E represents extinction coefficient, and when namely wavelength was 260nm, concentration was 0.1%(1mg/ml) suspension, the absorbance value when light path is 1cm.
Stripe disease virus E
0.1%1
Cm 260nmBe 3.21, rice stunt disease virus E
0.1%1
Cm 260nmBe 3.62, black streaked dwarf virus of rice virus E
0.1%1
Cm 260nmBe 3.16, yellow stunt of rice virus E
0.1%1
Cm 260nmBe 3.53.
With phosphate buffer respectively with stripe disease virus, rice stunt disease virus, black streak dwarf virus, yellow dwarf viral dilution as for 6*10-3mg/ml, artificial infection is in the of the right age blade that spreads diamond dust (500 order), and inoculation back water washes the blade that connects.
Treat that the blade face receive to do, cut, along arteries and veins in the blade to cuing open, about half leaf enter respectively in test compound solution and the aqua sterilisa, represent treatment combination blank group respectively, take out after 30 minutes, and place the cultivation of preserving moisture under matters temperature and the illumination, observed and recorded incidence after 3-4 days.
Choose the paddy rice lobus cardiacus of growing way unanimity, spread medicament at Zuo Banye gently with writing brush, right half leaf spreads aqua sterilisa and compares, after 12 hours, treat that blade is done after, virus inoculation.Dip in writing brush and to get viral juice, concentration is 6*10
-3Mg/ml, artificial frictional inoculation wipe 1-2 time along its offshoot direction gently at liquid level (full leaf) on the blade that spreads diamond dust, and the blade below is supported with palm or multilayer filter paper; The inoculation back scab namely occurs with the blade that flowing water (or wash bottle) flushing connects after 3-4 days, add up when scab is counted easily.
With medicament of the present invention and isopyknic viral juice mixing passivation 30min, a frictional inoculation paddy rice lobus cardiacus left side half leaf, aqua sterilisa and a viral juice combined inoculation left side half leaf recorded withered spot number after 3-4 days.
Select the paddy rice lobus cardiacus of growing way unanimity, dip in writing brush earlier and get viral juice, full leaf virus inoculation, the water flushing of inoculation back, treat that blade is done after, spread medicament at Zuo Banye, Zuo Banye spreads aqua sterilisa and does contrast, records withered spot number after 3-4 days.
Calculate inhibiting rate:
Inhibiting rate (%)=(not spreading the average withered spot number of medicament half leaf-average withered spot number of covering with paint medicament half leaf)/the do not spread average withered spot number of medicament half leaf
Wherein, the average withered spot number that does not spread medicament half leaf all adopts the mean of respectively organizing three repetitions with the average withered spot number that spreads medicament half leaf.
Each viroids test result of poly hexamethylene biguanide and salt pair paddy rice thereof such as following table (table 8, table 9, table 10, table 11).
Table 9. poly hexamethylene biguanide and salt thereof when 500ppm to the measurement result of stripe virus disease virus
Measurement result (table 9) shows that poly hexamethylene biguanide and salt pair stripe disease virus thereof have better protect and therapeutic action, and the Ningnanmycin effect of action effect and domestic treatment paddy rice virus usefulness commonly used at present is suitable.
Table 10. poly hexamethylene biguanide and salt thereof when 500ppm to the measurement result of rice stunt disease virus
Measurement result (table 10) shows that poly hexamethylene biguanide and salt pair rice stunt disease virus thereof have better protect and therapeutic action, and the Ningnanmycin effect of action effect and domestic treatment paddy rice virus usefulness commonly used at present is suitable.
Table 11. poly hexamethylene biguanide and salt thereof when 500ppm to the mensuration of black streaked dwarf virus of rice virus
Measurement result (table 11) shows that poly hexamethylene biguanide and salt pair black streaked dwarf virus of rice virus thereof have better protect and therapeutic action, and action effect will be higher than the domestic Moroxydine Hydrochloride for the treatment of paddy rice virus usefulness commonly used at present.
Table 12. poly hexamethylene biguanide and salt thereof when 500ppm to the measurement result of yellow stunt of rice virus
Measurement result (table 12) shows that poly hexamethylene biguanide and salt pair yellow stunt of rice virus thereof have better protect and therapeutic action, and action effect is better than contrast medicament Moroxydine Hydrochloride.
(2) the indoor determination of activity of poly hexamethylene biguanide or its salt pair wheat diseases
1, the indoor determination of activity of fungal diseases such as poly hexamethylene biguanide or its salt pair wheat scab, wheat powdery mildew, wheat rust, take-all, root rotof flax, wheat sharp eyespot, loose kernel smut, bunt.
Adopt the mycelial growth rate method.Dull and stereotyped central authorities connect a bacterium dish at pastille, and mycelia down.Support when above to the discontented culture dish of blank bacterium colony 2/3, measure colony diameter of each processing for 25 ℃.Each bacterium colony is measured 2 times by the right-angled intersection method, represents the size of bacterium colony with its mean.Calculate medicament to the inhibition of thalli growth rate, the concentration for the treatment of of poly hexamethylene biguanide salt is all in the active ingredient poly hexamethylene biguanide.
The blank colony diameter of mycelial growth inhibition rate %=(-chemicals treatment colony diameter)/(blank colony diameter-bacterium dish diameter) * 100
(1) poly hexamethylene biguanide is to the indoor determination of activity of wheat scab
The mycelial growth inhibition rate of table 13 poly hexamethylene biguanide or its salt pair wheat scab
Measurement result (table 13) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair wheat scab is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair wheat scab have the better prevention effect.
(2) the indoor determination of activity of poly hexamethylene biguanide or its salt pair wheat leaf rust
The mycelial growth inhibition rate of table 14 poly hexamethylene biguanide or its salt pair wheat leaf rust
Measurement result (table 14) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair wheat leaf rust is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair wheat leaf rust have the better prevention effect.
(3) the indoor determination of activity of poly hexamethylene biguanide or its salt pair wheat powdery mildew
The mycelial growth inhibition rate of table 15 poly hexamethylene biguanide or its salt pair wheat powdery mildew
Measurement result (table 15) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair wheat powdery mildew is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair wheat powdery mildew have the better prevention effect.
(4) the indoor determination of activity of poly hexamethylene biguanide or its salt pair wheat sharp eyespot
The mycelial growth inhibition rate of table 16 poly hexamethylene biguanide or its salt pair wheat sharp eyespot
Measurement result (table 16) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair wheat sharp eyespot is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair wheat sharp eyespot have the better prevention effect.
(5) the indoor determination of activity of poly hexamethylene biguanide or its salt pair take-all
The mycelial growth inhibition rate of table 17 poly hexamethylene biguanide or its salt pair take-all
Measurement result (table 17) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair take-all is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair take-all have the better prevention effect.
(6) poly hexamethylene biguanide or the indoor determination of activity of its salt pair loose smut of wheat
The mycelial growth inhibition rate of table 18 poly hexamethylene biguanide or its salt pair loose smut of wheat
Measurement result (table 18) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair loose smut of wheat is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair loose smut of wheat have the better prevention effect.
(7) the indoor determination of activity of poly hexamethylene biguanide or its salt pair bunt of wheat
The mycelial growth inhibition rate of table 19 poly hexamethylene biguanide or its salt pair bunt of wheat
Measurement result (table 19) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair bunt of wheat is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair bunt of wheat have the better prevention effect.
(8) the indoor determination of activity of poly hexamethylene biguanide or its salt pair root rotof flax
The mycelial growth inhibition rate of table 20 poly hexamethylene biguanide or its salt pair root rotof flax
Measurement result (table 20) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair root rotof flax is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair root rotof flax have the better prevention effect.
2, the indoor determination of activity of poly hexamethylene biguanide or its salt pair wheat bacterial stripe.
With pathogen at NA(peptone 5g, dusty yeast 1g, glucose 10g, agar 18g, distilled water 1L PH7.0-7.2) rules above the solid culture medium, cultivates up to growing single bacterium colony down at 28 degrees centigrade.The single bacterium colony of pathogen is to liquid NA medium on the picking NA solid culture medium, and is standby to exponential phase at 30 degrees centigrade, 180rpm constant temperature shaking table shaken cultivation.Medicament and contrast medicament are configured to the hot peptone 3g of certain density toxic NB(ox respectively, peptone 5g, dusty yeast 1g, grape is by 10g, 1L, PH7.0-7.2) liquid nutrient medium, get 5ml to test tube, add 40 μ L and contain the NA liquid nutrient medium of wheat bacterial leaf-blight, wheat bacterial stripe, wheat bacterial brown spot, the bacillary basal stem rot germ of wheat respectively, 30 degrees centigrade, 180rpm constant temperature shaking table shaken cultivation 2 days, the bacterium liquid of each concentration is measured OD value (600nm) at spectrophotometer.And measure the NB liquid nutrient medium OD value of drug concentration in addition, the OD value that medicament itself causes is proofreaied and correct.The computing formula of proofreading and correct OD value and preventive effect is as follows:
Proofread and correct OD value=contain bacterium OD value-aseptic culture medium OD value
Preventive effect %=(proofreaies and correct the toxic medium OD value of back control medium bacterium liquid OD value-proofread and correct)/control medium bacterium liquid OD value * 100 afterwards proofreaied and correct
Measure according to the method described above, the inhibition activity of part target compound sees the following form 21.
Table 21. medicament of the present invention suppresses indoor determination of activity to the wheat bacterial stripe
Measurement result (table 21) shows that the sick inhibition of poly hexamethylene biguanide or its salt pair wheat bacterial stripe is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair wheat bacterial stripe have the better prevention effect.
3, the indoor determination of activity of disease such as the yellow stunt of wheat viral disease that causes of poly hexamethylene biguanide or its salt pair wheat microorganism, wheat rosette stunt, stripe virus disease, black streak dwarf.
Adopt the Gooding method, choose more than 3 weeks of inoculation, respectively by yellow stunt of wheat viral disease, wheat rosette stunt, stripe virus disease, black streak dwarf virus systemic infection wheat upper blade, homogenate in phosphate buffer, double gauze filters, and 1000g is centrifugal, handle through 2 polyethylene glycol, centrifugal again, precipitation suspends with phosphate buffer, obtains the crude extract of each germ respectively.
Whole test is carried out under 4 degrees centigrade, and the absorbance with ultraviolet specrophotometer mensuration 260nm wavelength calculates virus concentration according to formula.
Virus concentration (mg/ml)=(A260* extension rate)/wherein E represents extinction coefficient, and when namely wavelength was 260nm, concentration was 0.1%(1mg/ml) suspension, the absorbance value when light path is 1cm.
Wheat stripe virus disease virus E
0.1%1
Cm 260nmBe 3.32, the wheat viral E of disease that stunts
0.1%1
Cm 260nmBe 3.53, wheat black streak dwarf virus E
0.1%1
Cm 260nmBe 3.75, yellow stunt of wheat virus E
0.1%1
Cm 260nmBe 3.28.
With phosphate buffer respectively with yellow stunt of wheat viral disease, wheat rosette stunt, stripe virus disease, black streak dwarf viral dilution as for 6*10-3mg/ml, artificial infection is in the of the right age blade that spreads diamond dust (500 order), the blade that the water flushing of inoculation back connects.
Treat that the blade face receive to do, cut, along arteries and veins in the blade to cuing open, about half leaf enter respectively in test compound solution and the aqua sterilisa, represent treatment combination blank group respectively, take out after 30 minutes, and place the cultivation of preserving moisture under matters temperature and the illumination, observed and recorded incidence after 3-4 days.
Choose the wheat lobus cardiacus of growing way unanimity, spread medicament at Zuo Banye gently with writing brush, right half leaf spreads aqua sterilisa and compares, after 12 hours, treat that blade is done after, virus inoculation.Dip in writing brush and to get viral juice, concentration is 6*10
-3Mg/ml, artificial frictional inoculation wipe 1-2 time along its offshoot direction gently at liquid level (full leaf) on the blade that spreads diamond dust, and the blade below is supported with palm or multilayer filter paper; The inoculation back scab namely occurs with the blade that flowing water (or wash bottle) flushing connects after 3-4 days, add up when scab is counted easily.
With medicament of the present invention and isopyknic viral juice mixing passivation 30min, a frictional inoculation wheat lobus cardiacus left side half leaf, aqua sterilisa and a viral juice combined inoculation left side half leaf recorded withered spot number after 3-4 days.
Select the wheat lobus cardiacus of growing way unanimity, dip in writing brush earlier and get viral juice, full leaf virus inoculation, the water flushing of inoculation back, treat that blade is done after, spread medicament at Zuo Banye, Zuo Banye spreads aqua sterilisa and does contrast, records withered spot number after 3-4 days.
Calculate inhibiting rate:
Inhibiting rate (%)=(not spreading the average withered spot number of medicament half leaf-average withered spot number of covering with paint medicament half leaf)/the do not spread average withered spot number of medicament half leaf
Wherein, the average withered spot number that does not spread medicament half leaf all adopts the mean of respectively organizing three repetitions with the average withered spot number that spreads medicament half leaf.
Each viroids test result of poly hexamethylene biguanide and salt pair wheat thereof such as following table (table 22, table 23, table 24, table 25).
Table 22. poly hexamethylene biguanide and salt thereof when 500ppm to the mensuration of yellow stunt of wheat viral disease virus
Measurement result (table 22) shows that poly hexamethylene biguanide and salt pair yellow stunt of wheat viral disease virus thereof have certain protection and inhibitory action, and the Ningnanmycin effect of action effect and domestic treatment wheat virus usefulness commonly used at present is suitable.
Table 23. poly hexamethylene biguanide and salt thereof when 500ppm to the measurement result of wheat rosette stunt virus
Measurement result (table 23) shows that poly hexamethylene biguanide and salt pair wheat rosette stunt virus thereof have better protect and inhibitory action, and the Ningnanmycin effect of action effect and domestic treatment wheat virus usefulness commonly used at present is suitable.
Table 24. poly hexamethylene biguanide and salt thereof when 500ppm to the mensuration of wheat stripe virus disease virus
Measurement result (table 24) shows that poly hexamethylene biguanide and salt pair wheat stripe virus disease virus thereof have certain protection and inhibitory action, and action effect is equivalent to the domestic Moroxydine Hydrochloride for the treatment of wheat virus usefulness commonly used at present.
Table 25. poly hexamethylene biguanide and salt thereof when 500ppm to the measurement result of wheat black streak dwarf
Measurement result (table 25) shows that poly hexamethylene biguanide and salt pair wheat black streak dwarf virus thereof have better protect and inhibitory action, and action effect is suitable with contrast medicament Moroxydine Hydrochloride.
(3) the indoor determination of activity of poly hexamethylene biguanide and salt pair maize diseases thereof
The indoor determination of activity of diseases such as poly hexamethylene biguanide or the large and small pinta of its salt pair control of maize, corn banded sclerotial blight, corn stalk rot disease, corn circle pinta, maize head smut.
1, poly hexamethylene biguanide is to the indoor determination of activity of the corn northern leaf blight
Table 26 poly hexamethylene biguanide is to the mycelial growth inhibition rate of the corn northern leaf blight, helminthosporium maydis
Measurement result (table 26) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair corn northern leaf blight, helminthosporium maydis is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair corn northern leaf blight, helminthosporium maydis have the better prevention effect.
2, poly hexamethylene biguanide is to the indoor determination of activity of corn banded sclerotial blight
Table 27 poly hexamethylene biguanide is to the mycelial growth inhibition rate of corn banded sclerotial blight
Measurement result (table 27) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair corn banded sclerotial blight is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair corn banded sclerotial blight have the better prevention effect.
3, poly hexamethylene biguanide is to the indoor determination of activity of corn stalk rot disease
The mycelial growth inhibition rate of table 28 poly hexamethylene biguanide or its salt pair corn stalk rot disease
Measurement result (table 28) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair corn stalk rot disease is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair corn stalk rot disease have the better prevention effect.
4, the indoor determination of activity of poly hexamethylene biguanide or its salt pair corn circle pinta
The mycelial growth inhibition rate of table 29 poly hexamethylene biguanide or its salt pair corn circle pinta
Measurement result (table 20) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair corn circle pinta is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair corn circle pinta has the better prevention effect.
5, the indoor determination of activity of poly hexamethylene biguanide or its salt pair maize head smut
The mycelial growth inhibition rate of table 30 poly hexamethylene biguanide or its salt pair maize head smut
Measurement result (table 30) shows that the mycelial growth inhibition of poly hexamethylene biguanide or its salt pair maize head smut is obvious, shows that poly hexamethylene biguanide of the present invention or its salt pair maize head smut have the better prevention effect.
Two, Application Example (field control effectiveness test)
The preparation that adopts the method for parallel test to verify to contain poly hexamethylene biguanide and salt thereof is to the field trial control efficiency of cereal crop disease
Grade scale:
0 grade: no scab;
1 grade: leaf spot lesion is less than 5, and length is less than 1cm;
3 grades: leaf spot lesion 6-10, part scab length is greater than 1cm;
5 grades: leaf spot lesion 11-25, the part scab is linked to be sheet, and lesion area accounts for the 10-25% of leaf area;
7 grades: leaf spot lesion is more than 26, and scab is linked to be sheet, and lesion area accounts for the 26-50% of leaf area;
9 grades: scab is linked to be sheet, lesion area account for leaf area more than 50% or full leaf withered.
1, poly hexamethylene biguanide is to the field control effectiveness test analysis of rice disease
(1) the field control effectiveness test analysis of poly hexamethylene biguanide or its salt pair rice blast
The field control effectiveness test analysis of table 31. poly hexamethylene biguanide or its salt pair rice blast
Field test results shows (table 31), and poly hexamethylene biguanide or its salt pair rice blast have shown the better prevention effect.
(2) field control effectiveness test of poly hexamethylene biguanide or its salt pair rice sheath blight disease
The field control effectiveness test analysis of table 32. poly hexamethylene biguanide or its salt pair rice sheath blight disease
Field test results shows (table 14), and poly hexamethylene biguanide or its salt pair rice sheath blight disease have shown the better prevention effect.
(3) field control effectiveness test of poly hexamethylene biguanide or its salt pair rice green smut
The field control effectiveness test analysis of table 32. poly hexamethylene biguanide or its salt pair rice green smut
Field test results shows (table 32), and poly hexamethylene biguanide or its salt pair rice green smut have shown the better prevention effect.
(4) field control effectiveness test of poly hexamethylene biguanide or its salt pair bakanae disease of rice
The field control effectiveness test analysis of table 33. poly hexamethylene biguanide or its salt pair bakanae disease of rice
Field test results shows (table 33), and poly hexamethylene biguanide or its salt pair bakanae disease of rice have shown the better prevention effect.
(5) field control effectiveness test of poly hexamethylene biguanide or its salt pair paddy bacterial disease
Table 34. poly hexamethylene biguanide or its salt pair bacterial leaf streak of rice field control effectiveness test are analyzed
Field test results shows (table 34), and poly hexamethylene biguanide or its salt pair bacterial leaf streak of rice have shown the better prevention effect.
(6) poly hexamethylene biguanide or its salt pair bacterial blight of rice field control effectiveness test
Table 35. poly hexamethylene biguanide or its salt pair bacterial blight of rice field control effectiveness test are analyzed
Field test results shows (table 35), and poly hexamethylene biguanide or its salt pair bacterial blight of rice have shown the better prevention effect.
(7) poly hexamethylene biguanide or its salt pair paddy bacterial brown spot field control effectiveness test
Table 36. poly hexamethylene biguanide or its salt pair paddy bacterial brown spot field control effectiveness test are analyzed
Field test results shows (table 36), and poly hexamethylene biguanide or its salt pair paddy bacterial brown spot have shown the better prevention effect.
(8) poly hexamethylene biguanide or its salt pair paddy bacterial basal stem rot field control effectiveness test
Table 37. poly hexamethylene biguanide or its salt pair paddy bacterial basal stem rot field control effectiveness test are analyzed
Field test results shows (table 37), and poly hexamethylene biguanide or its salt pair paddy bacterial basal stem rot have shown the better prevention effect.
(9) poly hexamethylene biguanide or its salt pair stripe disease field control effectiveness test
Table 38. poly hexamethylene biguanide or its salt pair stripe disease field control effectiveness test are analyzed
Field test results shows (table 38), and poly hexamethylene biguanide or its salt pair stripe disease have shown the better prevention effect.
(10) poly hexamethylene biguanide or its salt pair rice dwarf virus disease field control effectiveness test
Table 39. poly hexamethylene biguanide or its salt pair rice dwarf virus disease field control effectiveness test are analyzed
Field test results shows (table 39), and poly hexamethylene biguanide or its salt pair rice dwarf virus disease have shown the better prevention effect.
(11) poly hexamethylene biguanide or its salt pair black streaked dwarf virus of rice field control effectiveness test
Table 40. poly hexamethylene biguanide or its salt pair black streaked dwarf virus of rice field control effectiveness test are analyzed
Field test results shows (table 40), and poly hexamethylene biguanide or its salt pair black streaked dwarf virus of rice have shown the better prevention effect.
(12) poly hexamethylene biguanide or its salt pair yellow stunt of rice field control effectiveness test
Table 41. poly hexamethylene biguanide or its salt pair yellow stunt of rice field control effectiveness test are analyzed
Field test results shows (table 41), and poly hexamethylene biguanide or its salt pair yellow stunt of rice have shown the better prevention effect.
2, the field control effectiveness test analysis of poly hexamethylene biguanide or its salt pair wheat diseases
(1) the field control effectiveness test analysis of poly hexamethylene biguanide or its salt pair wheat scab
The field control effectiveness test analysis of table 42. poly hexamethylene biguanide or its salt pair wheat scab
Field test results shows (table 42), poly hexamethylene biguanide or its salt, and with more above-mentioned bactericide compounded wheat scab has been shown the better prevention effect.
(2) field control effectiveness test of poly hexamethylene biguanide or its salt pair wheat powdery mildew
The field control effectiveness test analysis of table 43. poly hexamethylene biguanide or its salt pair wheat powdery mildew
Field test results shows (table 43), poly hexamethylene biguanide or its salt, and bactericide compounded wheat powdery mildew has been shown the better prevention effect with more above-mentioned other.
(3) field control effectiveness test of poly hexamethylene biguanide or its salt pair wheat rust
Table 44. polyhexamethylene or its salt biguanides are to the field control effectiveness test analysis of wheat rust
Field test results shows (table 44), poly hexamethylene biguanide or its salt, and bactericide compounded wheat rust has been shown the better prevention effect with more above-mentioned other.
(4) field control effectiveness test of poly hexamethylene biguanide or its salt pair take-all
Table 45. poly hexamethylene biguanide or its salt pair take-all field control effectiveness test are analyzed
Field test results shows (table 45), and poly hexamethylene biguanide or its salt pair take-all have shown the better prevention effect.
(5) poly hexamethylene biguanide or its salt pair root rotof flax field control effectiveness test
Table 46. poly hexamethylene biguanide or its salt pair root rotof flax field control effectiveness test are analyzed
Field test results shows (table 46), and poly hexamethylene biguanide or its salt pair root rotof flax have shown the better prevention effect.
(6) poly hexamethylene biguanide or its salt pair wheat sharp eyespot field control effectiveness test
Table 47. poly hexamethylene biguanide or its salt pair wheat sharp eyespot field control effectiveness test are analyzed
Field test results shows (table 47), and poly hexamethylene biguanide or its salt pair wheat sharp eyespot have shown the better prevention effect.
(7) poly hexamethylene biguanide or its salt pair loose smut of wheat field control effectiveness test
Table 48 poly hexamethylene biguanide or its salt pair loose smut of wheat field control effectiveness test are analyzed
Field test results shows (table 48), and poly hexamethylene biguanide or its salt pair loose smut of wheat have shown the better prevention effect.
(8) poly hexamethylene biguanide or its salt pair bunt of wheat field control effectiveness test
Table 49. poly hexamethylene biguanide or its salt pair bunt of wheat field control effectiveness test are analyzed
Field test results shows (table 49), and poly hexamethylene biguanide or its salt pair bunt of wheat have shown the better prevention effect.
(9) poly hexamethylene biguanide or its salt pair wheat black streak dwarf field control effectiveness test
Table 50. poly hexamethylene biguanide or its salt pair wheat black streak dwarf field control effectiveness test are analyzed
Field test results shows (table 50), and poly hexamethylene biguanide or its salt pair wheat black streak dwarf have shown the better prevention effect.
(10) poly hexamethylene biguanide or its salt pair wheat stripe virus disease field control effectiveness test
Table 51. poly hexamethylene biguanide or its salt pair wheat stripe virus disease field control effectiveness test are analyzed
Field test results shows (table 51), and poly hexamethylene biguanide or its salt pair wheat stripe virus disease have shown the better prevention effect.
(11) poly hexamethylene biguanide or its salt pair yellow stunt of wheat field control effectiveness test
Table 52. poly hexamethylene biguanide or its salt pair yellow stunt of wheat field control effectiveness test are analyzed
Field test results shows (table 52), and poly hexamethylene biguanide or its salt pair yellow stunt of wheat have shown the better prevention effect.
(12) poly hexamethylene biguanide or its salt pair wheat bacterial stripe field control effectiveness test
Table 53. poly hexamethylene biguanide or its salt pair wheat bacterial stripe field control effectiveness test are analyzed
Field test results shows (table 53), and poly hexamethylene biguanide or its salt pair wheat bacterial stripe have shown the better prevention effect.
(13) poly hexamethylene biguanide or its salt pair wheat rosette stunt field control effectiveness test
Table 54. poly hexamethylene biguanide or its salt pair wheat rosette stunt field control effectiveness test are analyzed
Field test results shows (table 54), and poly hexamethylene biguanide or its salt pair wheat rosette stunt have shown the better prevention effect.
3, the field control effectiveness test analysis of poly hexamethylene biguanide or its salt pair maize diseases
(1) poly hexamethylene biguanide or its salt pair corn northern leaf blight field control effectiveness test
Table 55. poly hexamethylene biguanide or its salt pair corn northern leaf blight field control effectiveness test are analyzed
Field test results shows (table 55), and poly hexamethylene biguanide or its salt pair corn northern leaf blight have shown the better prevention effect.
(2) poly hexamethylene biguanide or its salt pair corn southern leaf blight field control effectiveness test
Table 56. poly hexamethylene biguanide or its salt pair corn southern leaf blight field control effectiveness test are analyzed
Field test results shows (table 56), and poly hexamethylene biguanide or its salt pair corn southern leaf blight have shown the better prevention effect.
(3) poly hexamethylene biguanide or its salt pair corn banded sclerotial blight field control effectiveness test
Table 57 poly hexamethylene biguanide or its salt pair corn banded sclerotial blight field control effectiveness test are analyzed
Field test results shows (table 57), and poly hexamethylene biguanide or its salt pair corn banded sclerotial blight have shown the better prevention effect.
(4) poly hexamethylene biguanide or its salt pair corn stalk rot disease field control effectiveness test
Table 58. poly hexamethylene biguanide or its salt pair corn stalk rot disease field control effectiveness test are analyzed
Field test results shows (table 58), and poly hexamethylene biguanide or its salt pair corn stalk rot disease have shown the better prevention effect.
(5) poly hexamethylene biguanide or its salt pair corn circle pinta field control effectiveness test
Table 59. poly hexamethylene biguanide or its salt pair corn circle pinta field control effectiveness test are analyzed
Field test results shows (table 59), and poly hexamethylene biguanide or its salt pair corn circle pinta have shown the better prevention effect.
(6) the sick field control effectiveness test of poly hexamethylene biguanide or its salt pair maize head smut
Table 60. poly hexamethylene biguanide or its salt pair maize head smut field control effectiveness test are analyzed
Field test results shows (table 60), and poly hexamethylene biguanide or its salt pair maize head smut have shown the better prevention effect.
(3) contain poly hexamethylene biguanide or its salt as kind clothing agent preparation and the application of active ingredient
1, plants the preparation of clothing agent
Each component and percentage by weight thereof constitute: hexamethylene 15%, fulvic acid 5%, sodium carboxymethylcellulose 0.3%, xanthans 0.3%, potassium dihydrogen phosphate 3%, defoamer 0.4%, warning colouration are that alkalescence is red 0.8%, water surplus.As above its manufacture craft flow process of the wheat seed coating agent of proportioning is:
A, feed intake: hexamethylene is poured in the reactor; Add again prepare alkaline red with
Fulvic acid adds water and xanthans, sodium carboxymethylcellulose afterwards; The revolving reaction still makes mixing of materials abundant;
B, stirring: by tubing pump the material in the reactor is delivered in the agitator tank, added defoamer, stir 10min;
C, preliminary grinding: the material in the agitator tank is sent in the 25L sand mill by feeding engine, carries out sand milling, and the material of outflow leaves in the agitator tank;
D, height are cut emulsification: by tubing pump product pump in the agitator tank is cut in the mulser to height, height is cut mulser fortune 10min;
E, correct grinding: the material that height is cut in the mulser pumps in the agitator tank, stirs 10min, and material is sent in the 50L refiner and finish grinded afterwards, makes particle footpath degree≤4 μ m of 90%, gets product.
2, use
The rice seed soaking test was carried out in the kind clothing agent that utilizes the present invention to make in 2012.Using method: paddy rice seed dressing is by the 1:20:1000(medicine: water: ratio seed) is carried out, artificial or machine seed dressing all can, mix thoroughly and dry the back and sow.To the cereal crop test of soaking seed, result of the test shows, the agent of hexamethylene kind clothing is 88.92% to the result that prevents and treats of bakanae disease of rice, the result that prevents and treats to rice blast is 85.12%, the result that prevents and treats to rice sheath blight disease is 88.21%, is 87.28% to the result that prevents and treats of bacterial blight of rice; Be 87.5% to wheat seed-borne disease, soil-borne disease as the result that prevents and treats to wheat sharp eyespot, the result that prevents and treats to take-all is 86.2%, the result that prevents and treats to root rotof flax is 86.9%, is 85.5% to the result that prevents and treats of loose kernel smut, is 87.4% to the result that prevents and treats of bunt.Being 89.18% to maize diseases as the result that prevents and treats to the corn banded sclerotial blight, is 88.98% to the result that prevents and treats of corn stalk rot disease, is 90.16% to the result that prevents and treats of maize head smut.
Claims (13)
1. a bactericide of preventing and treating the cereal crop disease is characterized in that the active ingredient of this bactericide comprises poly hexamethylene biguanide or its esters compound.
2. bactericide according to claim 1, the salt compounds that it is characterized in that poly hexamethylene biguanide are a kind of in hexamethylene, poly hexamethylene biguanide nitrate, poly hexamethylene biguanide carbonate, poly hexamethylene biguanide phosphate, poly hexamethylene biguanide sulphate, poly hexamethylene biguanide stearate, the poly hexamethylene biguanide acetate.
3. bactericide according to claim 1, the salt compounds of described poly hexamethylene biguanide is hexamethylene.
4. bactericide according to claim 1 is characterized in that this bactericide is made up of active ingredient poly hexamethylene biguanide or its esters compound and the conventional auxiliary agent of agricultural chemicals.
5. according to the described bactericide of each claim of claim 1-4, the formulation that it is characterized in that this bactericide is that agricultural goes up any one formulation that allows.
6. according to the bactericide of claim 5, the formulation of its this this bactericide of feature is aqua, wetting powder, soluble powder, microemulsion, aqueous emulsion, suspending agent, missible oil or water dispersible granules.
7. poly hexamethylene biguanide or its esters compound or the described bactericide of claim 1 are used for the purposes of control cereal crop disease.
8. according to the purposes of claim 7, it is characterized in that described cereal crop disease is selected from rice disease, wheat diseases or maize diseases.
9. purposes according to Claim 8 is characterized in that described cereal crop disease is selected from rice fungus diseases evil, paddy rice bacteriosis, paddy rice virus disease, wheat fungal diseases, wheat bacteriosis, wheat virus disease evil or maize diseases.
10. purposes according to Claim 8 is characterized in that described rice disease is selected from rice blast, rice sheath blight disease, rice green smut, bakanae disease of rice, bacterial blight of rice, bacterial leaf streak of rice, paddy bacterial brown spot, paddy bacterial basal stem rot, stripe disease, rice dwarf virus disease, black streaked dwarf virus of rice, yellow stunt of rice.
11. purposes according to Claim 8 is characterized in that described wheat diseases is selected from wheat scab, wheat powdery mildew, wheat rust, take-all, root rotof flax, wheat sharp eyespot, loose kernel smut, bunt, wheat bacterial stripe, yellow stunt of wheat viral disease, wheat rosette stunt, stripe virus disease or black streak dwarf.
12. purposes according to Claim 8 is characterized in that described maize diseases is selected from the corn northern leaf blight, corn southern leaf blight, corn banded sclerotial blight, corn stalk rot disease, corn circle pinta or maize head smut.
13. poly hexamethylene biguanide or its esters compound or the described bactericide of claim 1 are used for cereal crop seed-soaking or dressing, or spray more than the crop growth period root in case the application of the harmful aspect of curing the disease.
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