CN103224645A - Bleeding stopping sponge and preparation method thereof - Google Patents
Bleeding stopping sponge and preparation method thereof Download PDFInfo
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- CN103224645A CN103224645A CN 201310170559 CN201310170559A CN103224645A CN 103224645 A CN103224645 A CN 103224645A CN 201310170559 CN201310170559 CN 201310170559 CN 201310170559 A CN201310170559 A CN 201310170559A CN 103224645 A CN103224645 A CN 103224645A
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Abstract
The invention relates to a bleeding stopping sponge and an application thereof. The bleeding stopping sponge is prepared through redenaturation of denaturated starch in the prior art, and microwave processing. The bleeding stopping sponge has the advantages of excellent water absorption effect, safety and stability, is biocompatible, and can be directly applied to bleeding wounds.
Description
Technical field
The present invention relates to a kind of hematostatic sponge and preparation method thereof, particularly styptic sponge a kind of biocompatibility, that can be directly used in the blood surface of a wound and preparation method thereof.
Background technology
Hemorrhage is the phenomenon of easy appearance in accident and the medical procedure, and therefore a large amount of hemorrhage entail dangers to people's life security need in time stop blooding at the hemorrhage surface of a wound.
Along with the development of medical science, hemostatic material is of a great variety, and with regard to biocompatibility, biocompatible hemostatic material commonly used has: gelatin class hemostatic materials such as absorbability gelfoam, collagen protein sponge; SURGICEL, oxidized regenerated cellulose class hemostatic material; Natural biological polysaccharide hemostatic materials such as chitosan/chitin kind, starch polysaccharide etc.
Content of the present invention relates to the hemostatic material of starch polysaccharide, and the patent application that this field is relevant is as follows:
CN200710141944.0 denaturated starch absorbable hemostatic material and preparation method thereof, the CN200710199682.3 biocompatibility modified starch sponges, the modified starch material of CN200810032631.6 biocompatible hemostatic, anti, promoting healing, surgery sealing, modified starch that CN200810033239.3 is biocompatibility pre-gelatinized and preparation method thereof, CN200810009706.9 denaturated starch absorbable hemostatic material and preparation method thereof, CN200910045995.2 biocompatible hemostatic and promotion knitting material and preparation method thereof.
More than 6 patent applications, described content is roughly the same, is same applicant or contriver.The purposes of partially denaturing starch aspect hemostasis disclosed in the above-mentioned patent, and several specific modified starches have been proposed, as the hydroxypropyl Staragel 90V of the modified starch of cationic starch, epichlorohydrin cross-linked starch, carboxymethyl starch, hydroxyethylamyle, pre-gelatinization, pre-gelatinization, vinylformic acid-carboxymethyl starch graft copolymer etc., but its specific nature and detailed applications are not described.
The applicant has carried out systematic study to above-mentioned all kinds of modified starches, finds that the modified starch water absorbent rate in the above-mentioned patent application is low, and unstable properties can not satisfy clinical hematostatic demand.Therefore the applicant has carried out following processing to modified starch of the prior art: modified starch of the prior art is carried out sex change once more be prepared into through the modified starch after the sex change repeatedly, and carry out microwave treatment in this process.The applicant after modified starch being handled with microwave, surprised discovery, it can also improve the water absorbent rate and the rate of water absorption of modified starch.The applicant discovers through repeated experiments repeatedly, and the styptic sponge that the technical scheme by the application obtains can significantly improve its water-absorbent, has fabulous application prospect.The applicant has also filtered out preferred processing parameter by experiment simultaneously, its preparation more stable, and safety more benefits suitability for industrialized production.
Summary of the invention
An object of the present invention is to provide a kind of new styptic sponge, this material good water absorption, safety, stable performance, it is low to have overcome in the prior art modified starch water-absorbent, shortcomings such as preparation instability.
Another object of the present invention is to provide the preparation method of described styptic sponge, the objective of the invention is to be achieved through the following technical solutions:
A kind of preparation method of styptic sponge is characterized in that: may further comprise the steps:
1) the modified starch raw material is carried out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change,
2) drying, freeze-drying under the low temperature obtains styptic sponge.
Preferably, the modified starch raw material in the step 1 is one or more the combination in pre-gelatinized starch, denaturated starch by acid, dextrin, Sumstar 190, esterification starch, etherification starch, cross-linking starch, graft starch, the composite modified starch.
Preferably, before step 1, among or add the microwave treatment step afterwards.
Preferably, the power during microwave treatment is 750-850W.
Preferably, microwave treatment time is 3-5min.
Preferably, the temperature of microwave treatment processing is the 80-95 degree.
Another technical scheme of the present invention designs the purposes of the styptic sponge for preparing by method of the present invention:
Being used to prepare people, Mammals, birds, Reptilia has the hemostatic material of the blood surface of a wound.
Being used to prepare body surface, in-vivo tissue organ and body cavity inner tissue or organ has the blood surface of a wound or is used for hemostatic material under surgical operation, emergency care of trauma, laryngoscope, endoscope, the chamber mirror.
Be used to prepare the material that promotes organization healing.
Preferably, described tissue comprises sub-dermal soft tissue, muscle tissue, osseous tissue, cerebral tissue, nervous tissue, liver,kidney,spleen.
Be used for preparing the material that the tissue that prevents wound or organ and its hetero-organization on every side or organ stick together.
The applicant finds that method of the present invention has following characteristics after having carried out a series of conditional filtering tests:
Beneficial effect of the present invention:
1. method of the present invention has no requirement to the kind of modified starch, the modified starch of seeing commonly used may be used to present method in the prior art, its water absorbent rate of the styptic sponge for preparing and rate of water absorption have tangible performance advantage all far above modified starch sponges of the prior art.For example compare with control group, water absorbent rate on average improves more than 35%, and rate of water absorption improves more than 1 times, and tangible performance advantage is arranged.
The microwave step can be positioned at before the modified starch denaturation process, among or afterwards, as long as in the preparation whole process of modified starch, used the microwave step, all can improve the water absorbent rate and the rate of water absorption of the new modified starch of acquisition.
3. the present invention is by carrying out sex change again to existing modified starch, and microwave treatment in addition, further broken up the structure of starch molecule, significantly improved its water absorbing properties, and selected preferred processing parameter by a large amount of testing sieves, make the styptic sponge for preparing on water absorbing properties, be better than like product of the prior art, wide application prospect is arranged.Convenient, preparation more stable that styptic sponge of the present invention prepares, safety more benefits suitability for industrialized production.
Embodiment
Below in conjunction with embodiment the present invention is further described in detail, the embodiment that provides is only in order to illustrate the present invention, rather than in order to limit the scope of the invention.
Embodiment 1:
With the hydroxypropyl Staragel 90V through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, this modified starch slurry is placed under the microwave oven 80 degree 750W conditions again and heats 3min.Cooling back suction filtration with alcohol washing, drying, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 1.
Embodiment 2:
Place microwave oven 80 to spend under the 750W conditions in hydroxypropyl Staragel 90V slurry and heat 3min.Cooling back suction filtration, with alcohol washing, drying, again through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 2.
Embodiment 3:
With hydroxyethylamyle through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, this modified starch slurry is placed under the microwave oven 80 degree 750W conditions again and heats 3min.Cooling back suction filtration with alcohol washing, drying, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 3.
Embodiment 4:
Place microwave oven 80 to spend under the 750W conditions in the hydroxyethylamyle slurry and heat 3min.Cooling back suction filtration, with alcohol washing, drying, again through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 4.
Embodiment 5:
With carboxymethyl starch through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, this modified starch slurry is placed under the microwave oven 80 degree 750W conditions again and heats 3min.Cooling back suction filtration with alcohol washing, drying, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 5.
Embodiment 6:
Place microwave oven 80 to spend under the 750W conditions in the carboxymethyl starch slurry and heat 3min.Cooling back suction filtration, with alcohol washing, drying, again through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 6.
Embodiment 7:
With cationic starch through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, this modified starch slurry is placed under the microwave oven 80 degree 750W conditions again and heats 3min.Cooling back suction filtration with alcohol washing, drying, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 7.
Embodiment 8:
Place microwave oven 80 to spend under the 750W conditions in the cationic starch slurry and heat 3min.Cooling back suction filtration, with alcohol washing, drying, again through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 8.
Embodiment 9:
With the hydroxypropyl Staragel 90V through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, this modified starch slurry is placed under the microwave oven 95 degree 850W conditions again and heats 5min.Cooling back suction filtration with alcohol washing, drying, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 9.
Embodiment 10:
Place microwave oven 95 to spend under the 850W conditions in hydroxypropyl Staragel 90V slurry and heat 5min.Cooling back suction filtration, with alcohol washing, drying, again through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 10.
Embodiment 11:
With hydroxyethylamyle through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, this modified starch slurry is placed under the microwave oven 95 degree 850W conditions again and heats 5min.Cooling back suction filtration with alcohol washing, drying, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 11.
Embodiment 12:
Place microwave oven 95 to spend under the 850W conditions in the hydroxyethylamyle slurry and heat 5min.Cooling back suction filtration, with alcohol washing, drying, again through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 12.
Embodiment 13:
With carboxymethyl starch through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, this modified starch slurry is placed under the microwave oven 95 degree 850W conditions again and heats 5min.Cooling back suction filtration with alcohol washing, drying, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 13.
Embodiment 14:
Place microwave oven 95 to spend under the 850W conditions in the carboxymethyl starch slurry and heat 5min.Cooling back suction filtration, with alcohol washing, drying, again through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 14.
Embodiment 15:
With cationic starch through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, this modified starch slurry is placed under the microwave oven 95 degree 850W conditions again and heats 5min.Cooling back suction filtration with alcohol washing, drying, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 15.
Embodiment 16:
Place microwave oven 95 to spend under the 850W conditions in the cationic starch slurry and heat 5min.Cooling back suction filtration, with alcohol washing, drying, again through chemical reagent such as propylene oxide, phosphoric acid carry out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change, is lyophilize 15 hours under 15 handkerchiefs at-45 ℃, vacuum tightness, obtains styptic sponge 16.
Embodiment 17 control group experimental designs
Method according to the embodiment 1,3,4,5 of patent cn200710199682.3 prepares styptic sponge A respectively, C, D, E, group in contrast.
The water absorbent rate of embodiment 18 styptic sponges and the detection of rate of water absorption
Multiplying power that sponge sucks in water adopts centrifugal determination, is about to about 0.025g sponge as in the 2ml water, and balance 10min puts into whizzer then, under the 500rpm rotating speed, takes out after centrifugal 10 minutes, weighs and calculates the raffinate amount, and each sample test is removed mean value 6 times.
The rate of water absorption of sponge is observed by the German Dataphysics OCA40Micro of company video contact angle measurement.
Experimental result: the styptic sponge A that 16 kinds of hemostasis starch that makes by embodiment 1-16 method and embodiment 17 control groups obtain, C, D, its water absorbent rate of E and rate of water absorption contrast are as shown in the table:
Table 2
Interpretation:
Can draw following conclusion by above experimental data:
1. method of the present invention has no requirement to the kind of modified starch, the modified starch of seeing commonly used may be used to present method in the prior art, its water absorbent rate of the styptic sponge for preparing and rate of water absorption have tangible performance advantage all far above modified starch sponges of the prior art.For example compare with control group, water absorbent rate on average improves more than 30%, and rate of water absorption improves more than 1 times, and tangible performance advantage is arranged.
The microwave step can be positioned at before the modified starch denaturation process, among or afterwards, as long as in the preparation whole process of modified starch, used the microwave step, all can improve the water absorbent rate and the rate of water absorption of the new modified starch of acquisition.
Embodiment 4 modified starch styptic sponges are to the haemostatic effect of the hemorrhage model of liver
Test method:
Cut liver placenta percreta area 1.5cm * 2.5cm in the pig liver surface with scalpel, the surface of a wound degree of depth is 0.25cm, makes the hemorrhage surface of a wound, and the styptic sponge of employing has: styptic sponge 1-90, the styptic sponge that control group adopts has styptic sponge A, C, D, E.
Respectively the above-mentioned surface of a wound is stopped blooding.After hemorrhage, immediately styptic sponge is placed on the wound and also exerts pressure on sponge with medical surgical gloves or hemostatic gauze, blocking blood flow unclamps gloves or gauze behind 1～2min gently, observe haemostatic effect, gloves and gauze whether adhesion sponge and sludged blood, cause once more hemorrhage when opening.Need not after the hemostasis modified starch sponges is taken away or removed, get final product but do suitable infiltration flushing with physiological saline.
Test-results:
Styptic sponge 1-16 haemostatic effect is fine, and easy to use.All styptic sponges are met and are sucked blood immediately behind blood and form the sponge-sludged blood colloid of viscosity with blood, all can control the hemorrhage of the liver surface of a wound effectively within 1 minute, and styptic sponge A, C, D, E just can control over bleeding in the time more than 1.5 minutes.Styptic sponge 1-90 and liver wound tissue tight adhesion promote blood coagulation, and sludged blood does not stick together with the gauze dressing of pushing, and can not cause once more hemorrhage.
Claims (12)
1. the preparation method of a styptic sponge is characterized in that: may further comprise the steps:
1) the modified starch raw material is carried out etherificate, crosslinked, esterification is repeatedly made new modified starch after the sex change,
2) drying, freeze-drying under the low temperature obtains styptic sponge.
2. preparation method as claimed in claim 1 is characterized in that: the modified starch raw material in the step 1 is one or more the combination in pre-gelatinized starch, denaturated starch by acid, dextrin, Sumstar 190, esterification starch, etherification starch, cross-linking starch, graft starch, the composite modified starch.
3. as the described preparation method of claim 1-2, it is characterized in that: before step 1, among or add the microwave treatment step afterwards.
4. preparation method as claimed in claim 3 is characterized in that: the power during microwave treatment is 750-850W.
5. as the described preparation method of claim 3-4, it is characterized in that: microwave treatment time is 3-5min.
6. as the described preparation method of claim 3-5, it is characterized in that: the temperature that microwave treatment is handled is the 80-95 degree.
7. the styptic sponge for preparing of the described method of claim 1-6.
8. the purposes of the styptic sponge of claim 7, its feature exists: be used to prepare the hemostatic material that people, Mammals, birds, Reptilia have the blood surface of a wound.
9. the purposes of the styptic sponge of claim 7, its feature exists: being used to prepare body surface, in-vivo tissue organ and body cavity inner tissue or organ has the blood surface of a wound or is used for hemostatic material under surgical operation, emergency care of trauma, laryngoscope, endoscope, the chamber mirror.
10. the purposes of the styptic sponge of claim 7 is characterized in that: be used to prepare the material that promotes organization healing.
11. the purposes of the styptic sponge of claim 10 is characterized in that: described tissue comprises sub-dermal soft tissue, muscle tissue, osseous tissue, cerebral tissue, nervous tissue, liver,kidney,spleen.
12. the purposes of the styptic sponge of claim 7 is characterized in that: be used for preparing the material that the tissue that prevents wound or organ and its hetero-organization on every side or organ stick together.
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| CN 201310170559 CN103224645A (en) | 2013-05-10 | 2013-05-10 | Bleeding stopping sponge and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107245112A (en) * | 2017-07-12 | 2017-10-13 | 浙江大学 | A kind of preparation method of porous carboxy methyl amylose material |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107245112A (en) * | 2017-07-12 | 2017-10-13 | 浙江大学 | A kind of preparation method of porous carboxy methyl amylose material |
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Application publication date: 20130731 |