CN103223043A - Traditional chinese medicine composition - Google Patents
Traditional chinese medicine composition Download PDFInfo
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- CN103223043A CN103223043A CN2013101187665A CN201310118766A CN103223043A CN 103223043 A CN103223043 A CN 103223043A CN 2013101187665 A CN2013101187665 A CN 2013101187665A CN 201310118766 A CN201310118766 A CN 201310118766A CN 103223043 A CN103223043 A CN 103223043A
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Abstract
The invention relates to a natural pharmaceutical composition, a preparation method thereof and the usage in preparing an attenuated synergistic medicament in cancer chemotherapy, wherein the natural pharmaceutical composition consists of decoction condensed extract product of haw and terminalia, haw general flavoneI and 70% ethanol extract product of terminalia in a proportion of (3-5): (3-7): (5-8). The composition has good curative effect to cancer chemotherapy related diarrhoea and increases effect to cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition of being made up of Fructus Crataegi Fructus Chebulae water boiling concentration extract, Fructus Crataegi total flavones and Fructus Chebulae's 70% ethanol extraction, the preparation method of this natural drug composition and this natural drug composition are being used for preparing the purposes that reduces cancer chemotherapy dependency diarrhoea and treatment of cancer is played the medicine of potentiation.
Background technology
Malignant tumor is current harm humans existence, one of healthy principal disease, and its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.Chemotherapeutic no matter singly with or unite with toxicity such as all can cause that the patient suffers from diarrhoea, diarrhoea makes the chemotherapy dependency suffer from diarrhoea again behind the chemotherapy of tumors.Emerging and applying along with chemotherapeutics such as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc. in recent years, chemotherapy dependency diarrheal incidence rate improves year by year, and if do not carry out active treatment, easily cause patient's dehydration, malnutrition, serum electrolyte disorder, acid base imbalance etc., it is unfavorable greatly not only the rehabilitation of body to be brought, therefore also bring certain difficulty, to the important component part of chemotherapy dependency diarrheal effective treatment having become tumor patient wholistic therapy to CC.
Chemotherapy dependency diarrhoea is with regard to its clinical manifestation, surely belongs to the Chinese medicine category of " having loose bowels ", and Chinese medicine attracts people's attention for the research of having loose bowels, but still unmatchful anticancer disease chemotherapy has the Chinese patent medicine of alleviating diarrhoea and potentiation at present.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, the natural Chinese medicine that can be used for alleviating cancer chemotherapy dependency diarrhoea and playing the chemotherapeutic sensitivity effect that is easy to promote becomes clinical problem demanding prompt solution.
Fructus Crataegi another name Fructus Pyri Pashiae fruit, Rhododendron simsii Planch. fruit, it is long-pending to help digestion; Change the stasis of blood that stagnates.Cure mainly eating accumulation, dysentery.The Fructus Chebulae has the relieving diarrhea with astringents effect, is to control chronic diarrhea; Chronic dysentery; The medicine commonly used of proctoptosis, in recent years, Fructus Crataegi and Fructus Chebulae's antitumaous effect are confirmed, especially Fructus Crataegi total flavones and Fructus Chebulae's 70% ethanol extraction, the inventor has obtained optimal drug ratio compositions of the present invention at last through the research and the experiment of each a large amount of formula proportion, is the compositions of novelty, and proved that it has fabulous curative effect and can play potentiation to treatment of cancer cancer chemotherapy dependency diarrhoea, has finished the present invention thus.
Summary of the invention
A kind of natural drug composition provided by the invention, by Fructus Crataegi Fructus Chebulae water boiling concentration extract, Fructus Crataegi total flavones and Fructus Chebulae's 70% ethanol extraction by weight in 3-5: the ratio of 3-7: 5-8 is formed.
Above-mentioned natural drug composition, wherein the usage ratio of Fructus Crataegi Fructus Chebulae water boiling concentration extract, Fructus Crataegi total flavones and Fructus Chebulae's 70% ethanol extraction is 5: 7: 8.
Wherein, Fructus Crataegi Fructus Chebulae water boiling concentration extract, Fructus Crataegi total flavones, can be by well known to a person skilled in the art the conventional method acquisition of purifying, adopt these compositions that conventional method obtains or the natural drug of the present invention that adopts the commercial one-tenth of buying to be grouped into all can obtain identical curative effect.
Preferably, Fructus Crataegi Fructus Chebulae water boiling concentration preparation method of extract is in the above-mentioned natural drug composition:
According to proportion compatibility is 1: 1 or 1-4: 1 other ratios are got Fructus Crataegi, Fructus Chebulae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, promptly.
The preparation method of Fructus Crataegi total flavones is in the above-mentioned natural drug composition:
Get haw pulp, with 60% alcohol reflux 2 times, each 1.5h, merge extractive liquid, reclaims ethanol, extractum, extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, promptly.
Fructus Chebulae's 70% ethanol extraction preparation method is in the above-mentioned natural drug composition:
Get the Fructus Chebulae, drying is pulverized, and sieves, with 7-10 times of 70% soak with ethanol 12h, cross the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged centrifugal 15 minutes of 3000rpm, separation of supernatant, boil off ethanol, drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, promptly.
The present invention also provides the preferred manufacturing procedure of this natural drug composition, comprising:
(1) preparation of Fructus Crataegi Fructus Chebulae water boiling concentration extract
According to proportion compatibility is 1: 1 or 1-4: 1 other ratios are got Fructus Crataegi, Fructus Chebulae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, promptly.
(2) preparation of Fructus Crataegi total flavones
Get haw pulp, with 60% alcohol reflux 2 times, each 1.5h, merge extractive liquid, reclaims ethanol, extractum, extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, promptly.
(3) Fructus Chebulae's 70% ethanol extraction preparation
Get the Fructus Chebulae, drying is pulverized, and sieves, with 7-10 times of 70% soak with ethanol 12h, cross the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged centrifugal 15 minutes of 3000rpm, separation of supernatant, boil off ethanol, drying under reduced pressure becomes dry extract again, pulverizes, and sieves, promptly.
(4) choose Fructus Crataegi Fructus Chebulae water boiling concentration extract, Fructus Crataegi total flavones and Fructus Chebulae's 70% ethanol extraction in proportion, uniform mixing, promptly.
The preparation method of above-mentioned natural drug composition, also comprise: the product that step (4) makes is further made the capsule that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine, maybe will make the tablet that contains natural drug composition 100mg or 200mg specification by wet granulation technology.
The beneficial effect of natural drug composition of the present invention aspect anti-chemotherapy dependency diarrhoea and inhibition tumor is as follows:
1, natural drug composition of the present invention causes the diarrhea of mouse Study of model to show at irinotecan: the diarrhoea reaction for the irinotecan induced mice has tangible antagonism.
2, proved that this natural drug composition can strengthen the inhibitory action of chemotherapeutics to tumor, adopted mouse tumor model, observed this natural drug composition and cyclophosphamide combined inhibitory action mouse interior tumor.Studies show that: this natural drug composition can obviously increase the inhibitory action of cyclophosphamide for tumor.
3, the inventor makes up Fructus Crataegi Fructus Chebulae water boiling concentration extract, Fructus Crataegi total flavones and Fructus Chebulae's 70% ethanol extraction originally, and by the consumption proportion between definite these components of a large amount of experiments, make said composition bring into play good synergism, obtain unexpected effect.Pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention is at 3-5: 3-7: produced unexpected synergism during 5-8, potentiation is obvious, extraordinary effect is played in side effect, especially the usage ratio of each component be 5: 7: 8 o'clock especially obvious, this is that the inventor pays a large amount of creative works by keeping punching and obtains on the paper basis of delivering before, and those of ordinary skills only can't simply obtain this ratio range and optimum amount ratio by disclosed content.
4, this natural drug composition Fructus Crataegi Fructus Chebulae water boiling concentration extract, Fructus Crataegi total flavones and each component of Fructus Chebulae's 70% ethanol extraction all can obtain by conventional means, some of them component for example Fructus Crataegi total flavones and Fructus Chebulae's 70% ethanol extraction also can be buied by commerce, and such compositions itself has and obtains convenient, lower-cost effect.This also is the product that has splendid curative effect and higher industrial practicality concurrently that the inventor obtains by a large amount of creative work sides.
The specific embodiment
Further set forth the preparation of natural drug composition of the present invention below by embodiment and test example, with and the beneficial effect in diarrhea and antitumor, used.
Embodiment
The first step: the preparation of various extracts
(1) preparation of Fructus Crataegi Fructus Chebulae water boiling concentration extract
According to proportion compatibility is 1: 1 or 1-4: 1 other ratios are got Fructus Crataegi, Fructus Chebulae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, promptly.
(2) preparation of Fructus Crataegi total flavones
Get haw pulp, with 60% alcohol reflux 2 times, each 1.5h, merge extractive liquid, reclaims ethanol, extractum, extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, promptly.
(3) Fructus Chebulae's 70% ethanol extraction preparation
Get the Fructus Chebulae, drying is pulverized, and sieves, with 7-10 times of 70% soak with ethanol 12h, cross the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged centrifugal 15 minutes of 3000rpm, separation of supernatant, boil off ethanol, drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, promptly.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take by weighing Fructus Crataegi Fructus Chebulae water boiling concentration extract 25mg, Fructus Crataegi total flavones 35mg and the Fructus Chebulae 70% ethanol extraction 40mg of above-mentioned steps preparation respectively, take by weighing starch 395mg, magnesium stearate 5mg simultaneously, uniform mixing is filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take by weighing Fructus Crataegi Fructus Chebulae water boiling concentration extract 45mg, Fructus Crataegi total flavones 40mg and the Fructus Chebulae 70% ethanol extraction 15mg of above-mentioned steps preparation respectively, take by weighing starch 295mg, lactose 100mg simultaneously, uniform mixing is with wet granulation, drying, mix with the 5mg magnesium stearate behind the granulate, be pressed into tablet.
Drug effect and pharmacology's data:
Compositions is in following test example: the usage ratio of Fructus Crataegi Fructus Chebulae water boiling concentration extract, Fructus Crataegi total flavones and Fructus Chebulae's 70% ethanol extraction is 5: 7: 8.The inventor to above-mentioned each composition usage ratio at 3-5: 3-7: the compositions that the multiple proportioning combination in the time of between the 5-8 obtains has been carried out and last identical experiment, find that all the natural drug composition in this amount ranges can obtain good synergism, can play good effect and treatment for cancer is had potentiation cancer chemotherapy dependency diarrhoea.Below be the pharmacodynamics data that preferred embodiment obtains:
Test example 1: the pharmacodynamic study of natural drug composition treatment chemotherapy dependency diarrhoea effect
1, test material
(1) Fructus Crataegi, the Fructus Chebulae
(2) mice, male, ad lib drinking-water before the experiment.
2, test method:
Mice is divided into 5 groups at random, and 5 every group, the A group is model group, and the B group is Fructus Crataegi Fructus Chebulae water boiling concentration extract 10g.kg
-1(being equivalent to contain the crude drug amount), the C group is Fructus Crataegi total flavones 10g.kg
-1(being equivalent to contain the crude drug amount), D group Fructus Chebulae 70% ethanol extraction 10g.kg
-1(being equivalent to contain the crude drug amount), the E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to contain the crude drug amount), irinotecan intraperitoneal injection (75mg/kg), continuous 4 days, duplicates chemotherapy dependency diarrhoea model at every day 1 time.B, C, D, E group were irritated stomach 8 days with corresponding extract continuously, and mice is placed metabolic cage, spread white filter paper at the bottom of the cage with observation stool situation, and observed mice crissum situation and Mus tail fecal pollution situation, stimulated the anus defecation in conjunction with cotton swab, and every day, observed and recorded was 2 times.Diarrhoea degree standard scoring is as follows: 0 minute: stool is normal or do not have; 1 minute: laxativeness, stool is visible slight wet soft; 2 minutes: the wet and not molding of moderate diarrhoea, stool.And there is slight crissum painted; 3 minutes, severe diarrhoea, watery stool is also painted with the severe crissum.
3, experimental result
Compare with model group, Fructus Crataegi Fructus Chebulae water boiling concentration extract group chemotherapy dependency diarrheal degree is lower than model group, and natural drug composition group of the present invention diarrhoea incidence rate obviously alleviates.The results are shown in Table 1.
Table 1 present composition is to mice chemotherapy dependency diarrhoea incidence rate and scoring influence
Annotate: a is equivalent to contain the crude drug amount
Test example 2: the pharmacodynamic study of natural drug composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before the experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180The inhibition test of sarcoma growth
Employing was gone down to posterity 5-7 days, and well-grown milky ascites is diluted to tumor cell suspension with an amount of physiological saline solution, and cell number is 10
7/ mL, 5 every group, the right axillary fossa subcutaneous vaccination of every Mus 0.2mL (contains oncocyte 2 * 10
6Individual).Inoculation random packet next day, experimental group natural drug composition of the present invention is divided into (200,100mg/mL) two dosage groups, every Mus is irritated stomach 0.5ml, gives cyclophosphamide 10gkg simultaneously
-1D
-1Negative control group gives 0.5mL distilled water; The positive drug group gives cyclophosphamide 10gkg
-1D
-1, successive administration 10 days.The conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all takes off cervical vertebra puts to death, and strips subcutaneous solid tumor tumor piece, weighs, and calculates tumor control rate (%) by following formula:
Tumor control rate=(the average tumor of the matched group average tumor of heavy (C)-experimental group heavy (T))/average tumor of matched group heavy (C)
3, result of the test
Natural drug composition of the present invention is low, high dose group all can strengthen the cyclophosphamide agent with to S
180The effect of inhibition degree, especially the strongest with the high dose group effect.(as table 2)
Table 2 natural drug composition of the present invention is to S
180The tumor inhibition effect of mice
Claims (5)
1. natural drug composition that reduces cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation, it is characterized in that said composition by Fructus Crataegi Fructus Chebulae water boiling concentration extract, Fructus Crataegi total flavones and Fructus Chebulae's 70% ethanol extraction by weight in 3-5: the ratio of 3-7: 5-8 is formed
Wherein Fructus Crataegi Fructus Chebulae water boiling concentration preparation method of extract is:
According to proportion compatibility is 1: 1 or 1-4: 1 other ratios are got Fructus Crataegi, Fructus Chebulae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, promptly;
Wherein the preparation method of Fructus Crataegi total flavones is:
Get haw pulp, with 60% alcohol reflux 2 times, each 1.5h, merge extractive liquid, reclaims ethanol, extractum, extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, promptly;
Wherein the preparation method of Fructus Chebulae's 70% ethanol extraction is:
Get the Fructus Chebulae, drying is pulverized, and sieves, with 7-10 times of 70% soak with ethanol 12h, cross the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged centrifugal 15 minutes of 3000rpm, separation of supernatant, boil off ethanol, drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, promptly.
2. natural drug composition as claimed in claim 1, wherein the usage ratio of Fructus Crataegi Fructus Chebulae water boiling concentration extract, Fructus Crataegi total flavones and Fructus Chebulae's 70% ethanol extraction is 5: 7: 8.
3. as the preparation method of the natural drug composition of claim 1 or 2, it is characterized in that this method comprises:
(1) preparation of Fructus Crataegi Fructus Chebulae water boiling concentration extract
According to proportion compatibility is 1: 1 or 1-4: 1 other ratios are got Fructus Crataegi, Fructus Chebulae, 10 times of amount distilled water immersion 1h, heating decocts 1h on the electric furnace that adds asbestos gauge, and silk filters, preserve filtrate, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, and twice filtrate is mixed, heating is concentrated into small size, ultrafiltration membrance filter adopts 70 ℃ of drying under reduced pressure 36h of vacuum drying oven to get pale brown toner end, promptly;
(2) preparation of Fructus Crataegi total flavones
Get haw pulp, with 60% alcohol reflux 2 times, each 1.5h, merge extractive liquid, reclaims ethanol, extractum, extractum adds an amount of distilled water respectively, stirs evenly, and adds CaCl
21g, standing over night, centrifugal, collect supernatant, transfer pH to 7～8 with ammonia, add in the macroporous adsorptive resins, regulate flow velocity 5mL/min, with distilled water flush away impurity, be the mobile phase desorption with ethanol, reclaim ethanol, promptly;
(3) preparation of Fructus Chebulae's 70%7 alcohol extracts
Get the Fructus Chebulae, drying is pulverized, and sieves, with 7-10 times of 70% soak with ethanol 12h, cross the leaching clear liquor, residue continues to soak 12h, and twice filtrate is merged centrifugal 15 minutes of 3000rpm, separation of supernatant, boil off ethanol, drying under reduced pressure becomes dry extract again, pulverizes, and crosses 100 mesh sieves, promptly;
(4) choose Fructus Crataegi Fructus Chebulae water boiling concentration extract, Fructus Crataegi total flavones and Fructus Chebulae's 70% ethanol extraction uniform mixing in proportion, promptly.
4. the preparation method of natural drug composition as claimed in claim 3, also comprise: the product that step (4) makes is further made the capsule that contains natural drug composition 100mg or 200mg specification by the pharmacy means of routine, maybe will make the tablet that contains natural drug composition 100mg or 200mg specification by wet granulation technology.
5. as the purposes of each described natural drug composition of claim 1-2 in preparing the medicine that reduces the reaction of cancer chemotherapy dependency diarrhoea and the treatment cancer is played potentiation.
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| CN101352560A (en) * | 2008-08-29 | 2009-01-28 | 温先敏 | Medicament for treating tumor |
| CN101564527A (en) * | 2009-06-01 | 2009-10-28 | 刘岑 | Chinese medicament and western medicament combined formulation for treating cancerous diarrhea and processing method thereof |
| KR20110048133A (en) * | 2009-11-02 | 2011-05-11 | 연세대학교 산학협력단 | Terminalia chebula extract for preventing or treating prostate cancer |
| US20110236324A1 (en) * | 2010-03-23 | 2011-09-29 | Namita Deo | Plant extracts, compositions containing same, and uses thereof |
-
2013
- 2013-03-27 CN CN2013101187665A patent/CN103223043A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101352560A (en) * | 2008-08-29 | 2009-01-28 | 温先敏 | Medicament for treating tumor |
| CN101564527A (en) * | 2009-06-01 | 2009-10-28 | 刘岑 | Chinese medicament and western medicament combined formulation for treating cancerous diarrhea and processing method thereof |
| KR20110048133A (en) * | 2009-11-02 | 2011-05-11 | 연세대학교 산학협력단 | Terminalia chebula extract for preventing or treating prostate cancer |
| US20110236324A1 (en) * | 2010-03-23 | 2011-09-29 | Namita Deo | Plant extracts, compositions containing same, and uses thereof |
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| VAIBHAV AHER ET AL: "Immunomodulatory Activity of Alcohol Extract of Terminalia chebula Retz Combretaceae", 《TROP J PHARM RES》, vol. 10, no. 5, 31 December 2011 (2011-12-31), pages 567 - 575 * |
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Application publication date: 20130731 |