CN103215188A - Method for microalgae breeding and biological oil co-production - Google Patents
Method for microalgae breeding and biological oil co-production Download PDFInfo
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Abstract
The present invention relates to a novel method for microalgae circulation breeding and biological oil co-production, wherein additional nutrition substances are not required to be added, and the method can be provided for low cost microalgae culture and biological oil co-production. The method mainly comprises a novel microalgae circulation breeding manner and application of the circulation breeding process to co-produce the biological oil, wherein the microalgae collected from each generation breeding is subjected to hydrolysis to obtain a water phase and an oil phase, the water phase is adopted as a microalgae circulation breeding nutrition liquid to be added to a microalgae breeding system so as to provide a carbon source, a nitrogen source, a phosphorus source and inorganic salts, and achieve a purpose of circulation breeding, and fatty acids contained in the oil phase are the products. The method for microalgae breeding has characteristics of high efficiency, rapid microalgae growth, no requirement of additional nitrogen source, phosphorus source and inorganic salts, biological oil co-production, high economic values and high industrial application prospects.
Description
Technical field
The invention belongs to the both culturing microalgae field, specifically, relate to a kind of cultivation of little algae and other photosynthetic organism cells and method of co-producing bio-oil of being suitable for.
Background technology
Exhausted day by day now at fossil resource, seek alternative green energy resource and be subjected to extensive attention.And little algae to be a class have Biological resources that major application is worth.
At present, the nutritional mode of little algae mainly contains three kinds of light autotrophy, difference and mixotrophisms.Light autotrophy pattern adopts sunlight as the energy, and absorbing carbon dioxide adds inorganic salt and cultivates as inorganic carbon source in nutritive medium.Also can adopt organism such as glucose to make little algae heterotrophic growth.Mixotrophism adds organism and cultivates little algae in bioreactor.It is low that the light autotrophy of little algae is cultivated cost, and frustule quality better, but need to add inorganic salt and nitrogenous source phosphorus source exists also in addition that cell density is low, growth efficiency is low, is vulnerable to pollute and is subjected to shortcoming such as weather effect.The heterotrophism incubation growth efficient height of little algae, algae cell density is big, but also needs to add organic carbon source except needs add inorganic salt and nitrogenous source phosphorus source, and the frustule quality that heterotrophism is cultivated is low, and can not absorbing carbon dioxide.And mixotrophism can either utilize the sunlight absorbing carbon dioxide can keep higher growth efficiency.
Chinese patent CN101838606A has proposed the little algae autotrophy of sewage disposal carbon emission reduction air-lift loop-heterotrophism coupling light bio-reactor, autotrophy and peculiar coupled pattern have been adopted, by increasing a heterotrophism district, make solid carbon efficiencies height, little algae cultivate the concentration height, be fit to the high-efficient culture of sewage disposal carbon emission reduction and little algae large-scale low-cost.Chinese patent CN102154110A has proposed a kind of microalgae culture method of high yield, little algae algae kind is carried out heterotrophism cultivate, and cultivates the frustule that is obtained with heterotrophism then and cultivates as the light autotrophy of seed.Can solve the low problem of growth efficiency in the extensive light autotrophy cultivation of little algae.Chinese patent CN101285075A has proposed the coupling process of a kind of biogas fermentation and autotrophic freshwater microalgae cultivation, with the substratum that natural pond liquid is cultivated as little algae, the CO in the biogas
2Cultivate required carbon source as autotrophic freshwater microalgae.Acid solution after Chinese patent CN101921811A finishes biogas fermentation is carried out the heterotrophism cultivation as substratum to little algae, and the gas mixture circulation of generation is passed into the autotrophy of ventilating in the photo-biological reactor and cultivates.Chinese patent CN101914572A proposes a kind of method of carbon dioxide zero discharge type organic waste recovery energy, with the natural pond liquid behind the biogas fermentation directly as the full nutritional medium of little algae, natural pond liquid and inoculation algae liquid mix the laggard bioreactor of going into, and utilize the CO that comes from carbon dioxide storage tank
2Be carbon source, carry out with photoautotrophy growth fixation CO
2Little algae of mode is cultivated.Chinese patent CN101575567A has proposed a kind of method and reactor thereof of culturing microalgae by illumination way, under 1%～40% carbonic acid gas situation of feeding, cultivates 5 day time acquisition fine algae dry cell weight concentration and reaches more than the 3.5g/L.Chinese patent CN101979498A has proposed a kind of little algae high yield heterotrophism cultured method, adopts semi-continuous training method, in culturing process, emits a part of algae liquid, adds the peculiar substratum and the sterilized water of equal volume simultaneously, realizes the high yield cultivation.Chinese patent CN102021208A has proposed greasy method in the little gonidium of a kind of quick accumulation, cultivates carrying out photoinduction after the dilution of heterotrophism cultured microalgae algae liquid, and little algae is accumulated fast at photoinduction stage grease.Chinese patent CN102089434A has proposed a kind of integrated system of biofuel raw material production, utilizes organic carbon and nutritive substance to be used for the heterotrophism seed culture, and little algae algae kind of gained is carried out extensive autotrophy cultivation then, is used for the cellular biomass accumulation.Chinese patent CN102174409A has proposed the method that a kind of mixotrophism is cultivated rapid growth of chlorella and a large amount of accumulation bio-oils, first section is adopted the chlorella autotrophy to cultivate acquisition than high-biomass, second section is adopted the organic ventilation heterotrophism training mode of interpolation, accumulates grease on large number of biological amount basis fast.Chinese patent CN101280328A proposed a kind of from the autotrophy to the heterotrophism two steps cultivate chlorella production method of bio-diesel oil, spissated autotrophy algae is changed over to carries out heterotrophic growth in the fermentor tank, make it synthetic neutral fat.Biomass can reach 108g/L, fat content can reach 52% of dry cell weight.Chinese patent CN1446882A cultivates heterotrophism algae fast pyrogenation with amylorrhexis for one kind and prepares method of bio-diesel oil, with the low-quality foodstuff starch is raw material, enzymolysis starch system glucose obtains heterophytic chlorella by the heterotrophism transformation technology, and the frustule preparation cost reduces 3-4 doubly; Lipid content is higher than autotrophy algae 3-4 doubly.Chinese patent CN101549932A has proposed a kind of production method of organic sewage waste residue treatment, microalgae culturing and oil refining, utilize anaerobic biological technical point other places reason organic sewage and organic waste residues, again sewage and natural pond liquid are carried out after aerobic biochemical handles, be deployed into nutrient solution, feed the CO 2 waste gas breed oil-containing micro-algae that biogas burning back produces.Chinese patent CN102161550A has proposed a kind of livestock and poultry cultivation sewage and has been used for the method for producing fodder additives and being cleaned into water, natural pond liquid after the livestock and poultry cultivation sewage disposal is gone into bioreactor and is carried out both culturing microalgae by ultrafiltration membrance filter is laggard, and isolated little algae slurry enters fermentation/enzymatic vessel (pond) and make fodder additives behind fermentation/enzymolysis.Chinese patent CN200610089354.3 has proposed a kind of Vegetable oil lipoprotein of using or/and the method for animal grease system alkene prepares ethene, propylene and butylene with Vegetable oil lipoprotein or/and animal grease is the catalytic material cracking.Chinese patent CN200710099839.5 has proposed a kind of Vegetable oil lipoprotein or/and the catalysis conversion method of animal grease, Vegetable oil lipoprotein carries out catalytic cracking reaction or/and the animal grease raw material contacts with the catalyzer of the β zeolite that contains modification in compound reactor, obtain purpose product low-carbon alkene and gasoline, diesel oil, heavy oil.
The primary product that present little algae transforms is fuel products such as biofuel, bio oil and gas.Bio oil in little algae being converted into the large Chemicals of low-carbon alkene such as ethene, propylene, butylene is a new research direction.
The organic carbon that prior art all fails to solve in the low and heterotrophism of light autophyting growth efficient relies on outside situation about adding.
Summary of the invention
For overcoming a difficult problem of the prior art, the invention provides a kind of little algae and the cultivation of other photosynthetic organism cells and method of co-producing bio-oil and alkene of being fit to.Method of the present invention utilizes bioreactor to carry out the cyclic culture of little algae, a certain amount of per generation is cultured little algae of collecting to be hydrolyzed under certain temperature and pressure, obtain water and oil phase by separation, water joins the cultivation of carrying out little algae in the cultivating system of future generation as nutritive medium, and oil phase is as product.Advantage of the present invention is to need not additionally to add nutrition in the cyclic culture process of little algae.
The method of little algae cyclic culture of the present invention and co-producing bio-oil (below abbreviate " method of the present invention " as) may further comprise the steps:
1) utilizes bioreactor to carry out the breed of little algae, will culture the little algae collect acquisition hydrolyzed solution that under the pressure of 50 ℃-250 ℃ temperature and 0.1MPa-4.0MPa, is hydrolyzed at every turn;
2) hydrolyzed solution that step 1) is obtained obtains water and oil phase by separation, and described water joins as nutritive medium that the breeding for little algae provides nutrition in the both culturing microalgae of future generation system, and oil phase is as product;
Wherein in the cyclic culture process of little algae, do not need additionally to add nutrition, need the inorganic salt and the nitrogenous source phosphorus source of adding in especially little algae autotrophy mode.
In the preferred embodiment of method of the present invention, little algae can be marine microalgae or freshwater microalgae.
In a preferred embodiment of method of the present invention, bioreactor can be duct type or the box reactor of plate, and the circulation of little algae nutrient solution can be adopted pump circulation or gas lift circulation.
In a preferred embodiment of method of the present invention, the light source that bioreactor adopts can mix, be used alternatingly for luminescent lamp, fluorescent lamp, sunlight or with their.
In a preferred embodiment of method of the present invention, before hydrolysis, little algae of collecting can be processed into the form of dried microalgae powder or wet little algae.
In a preferred embodiment of method of the present invention, the method for hydrolysis of little algae can be the acid hydrolysis under the normal pressure, neutrallty condition hydrolysis under the High Temperature High Pressure or the acid hydrolysis under the High Temperature High Pressure.
In a preferred embodiment of method of the present invention, the separation method of water and oil phase can be separatory, filtration or extraction.
In a preferred embodiment of method of the present invention, used acid can be selected from one or more in hydrochloric acid, sulfuric acid, the phosphoric acid in the acid hydrolysis, and concentration can be 0.1mol/L-2mol/L.
In another preferred embodiment of method of the present invention, the condition of High Temperature High Pressure can be 100 ℃-250 ℃ temperature and the pressure of 0.1MPa-4.0MPa.Acid hydrolysis temperature under the normal pressure can be for 50 above below 100 ℃, preferred 50～95 ℃.The neutrallty condition hydrolysis refers in water or diluted alkaline (for example sodium hydroxide solution of 5～10wt%) in be hydrolyzed.
In little algae of conventional light autotrophy mode is cultivated, also need usually to add SODIUMNITRATE as nitrogenous source; Add SODIUM PHOSPHATE, MONOBASIC as the phosphorus source; Add inorganic salt such as sylvite, sodium salt, calcium salt, molysite, zinc salt, manganese salt, magnesium salts, molybdenum salt, mantoquita, cobalt salt.In the cultivation of heterotrophism mode, also need from the outside constantly additionally to add organic carbon source to keep the growth of little algae usually.
And the method according to this invention, amino acid, glucose and the glycerine that the aqueous phase that the separation hydrolyzed solution obtains comprises can be used as the carbon source of micro algae growth of future generation; Amino acid can be used as nitrogenous source; Water miscible phosphoric acid salt can be used as the phosphorus source; Water miscible sylvite, sodium salt, calcium salt, molysite, zinc salt, manganese salt, magnesium salts, molybdenum salt, mantoquita, cobalt salt etc. can be used as the inorganic salt composition.
In addition, in the method for the invention, mainly comprise lipid acid in the oil phase that the separation hydrolyzed solution obtains, it reclaims as the bio oil product.
Beneficial effect of the present invention:
1. the organic carbon that method of the present invention has solved in the low and heterotrophism of light autophyting growth efficient relies on outside situation about adding, and can co-producing bio-oil.
2. method of the present invention does not need additionally to add inorganic nutrient salt and organic carbon in both culturing microalgae, the growth efficiency height, and fast growth has been realized the mode of cyclic culture.
Embodiment
Come further to illustrate the present invention by the following examples.But should be appreciated that described embodiment is illustrational purpose, and be not intended to limit the scope of the invention and spirit.
Comparative example
Starting point concentration is 5 * 10
6In the 10L micro algae culturing liquid that individual cell is every milliliter, add the f/2 nutrient solution of standard.Contain 75mg NaNO in making every liter
3, 5mg NaH
2PO
4H
2O.3.15mgFeCl
36H
2O, 4.36mg Na
2EDTA, 0.0098mg CuSO
45H
2O, 0.0063mgNa
2MoO
42H
2O, 0.022mg ZnSO
47H
2O, 0.01mg CoCl
26H
2O, 0.18mgMnCl
24H
2O.0.001mg vitamins B
12, 0.2mg VITMAIN B1,0.001mg vitamin H. and photo-biological reactor is that hard silicon boron glass is made, and uniform distribution 2-4 props up the 30W fluorescent tube bulb around the reactor, and water bath with thermostatic control is controlled at 25-30 ℃, feeds to contain 3wt%CO
2Air Mixture, gas flow are 200ml/min.Cultivate that density is 1g/L after 7 days, obtain algae powder 10g after collecting drying.
Embodiment 1
The golden algae powder 18g that collects, the hydrochloric acid soln 90ml of adding 5% (weight), normal pressure and temperature heat 2hr down for 50 ℃, filter, and obtain filter cake 6.3g, percent hydrolysis 65%.Filter cake is obtained extract 3.3g with n-hexane extraction, for showing slightly the bio oil of black.It is 5 * 10 that filtrate is added starting point concentration with in 10% (weight) NaOH aqueous solution and back
6In the 10L micro algae culturing liquid that individual cell is every milliliter, photo-biological reactor is the box reactor of plate that hard silicon boron glass is made, and uniform distribution 2-4 props up the 30W fluorescent tube bulb around the reactor, and water bath with thermostatic control is controlled at 25-30 ℃, feeds to contain 3wt%CO
2Air Mixture, gas flow are 200ml/min.Micro algae growth is rapid, cultivates that density is 4 * 10 after 3 days
7Every milliliter in individual cell, density is 1.77g/L after 7 days, obtains algae powder 17.7g after the collection drying.
Embodiment 2
The bead algae powder 18g that collects, the hydrochloric acid soln 90ml of adding 5% (weight), 100 ℃ of following reflux 2hr of normal pressure and temperature obtain filter cake 7.3g, percent hydrolysis 59.4%.With the filter cake n-hexane extraction,, obtain the 1.82g product with the normal hexane rotary evaporation.It is 5 * 10 that filtrate is added starting point concentration with in the 10%NaOH aqueous solution and back
6In the 10L micro algae culturing liquid that individual cell is every milliliter, photo-biological reactor is the pipeline reactor that hard silicon boron glass is made, and uniform distribution 2-4 props up the 30W fluorescent tube bulb around the reactor, and water bath with thermostatic control is controlled at 25-30 ℃, feeds to contain 3wt%CO
2Air Mixture, gas flow are 200ml/min.Micro algae growth is rapid, cultivates that density is 1.69g/L after 7 days, obtains algae powder 16.9g after collecting drying.
Embodiment 3
The golden algae powder 18g that collects, the sulphuric acid soln 90ml of adding 5%, normal pressure and temperature heat 2hr down for 80 ℃, filter, and obtain filter cake 7.7g, percent hydrolysis 57%.Filter cake is obtained extract 3.0g with n-hexane extraction, for showing slightly the bio oil of black.It is 5 * 10 that in the filtrate and back is added starting point concentration
6In the 10L micro algae culturing liquid that individual cell is every milliliter, photo-biological reactor is that hard silicon boron glass is made, and uniform distribution 2-4 props up the 30W fluorescent tube bulb around the reactor, and water bath with thermostatic control is controlled at 25-30 ℃, feeds to contain 3wt%CO
2Air Mixture, gas flow are 200ml/min.Micro algae growth is rapid, cultivates that density is 3.4 * 10 after 3 days
7Every milliliter in individual cell, density is 1.47g/L after 7 days, obtains algae powder 14.7g after the collection drying.
Embodiment 4
The golden algae powder 18g that collects, the phosphoric acid solution 90ml of adding 5%, normal pressure and temperature heat 2hr down for 95 ℃, filter, and obtain filter cake 10.9g, percent hydrolysis 39%.Filter cake is obtained extract 2.1g with n-hexane extraction, for showing slightly the bio oil of black.It is 5 * 10 that in the filtrate and back is added starting point concentration
6In the 10L micro algae culturing liquid that individual cell is every milliliter, photo-biological reactor is that hard silicon boron glass is made, and uniform distribution 2-4 props up the 30W fluorescent tube bulb around the reactor, and water bath with thermostatic control is controlled at 25-30 ℃, feeds to contain 3wt%CO
2Air Mixture, gas flow are 200ml/min.Micro algae growth is rapid, cultivates that density is 2 * 10 after 3 days
7Every milliliter in individual cell, density is 0.89g/L after 7 days, obtains algae powder 8.9g after the collection drying.
Embodiment 5
The golden algae powder 18g that collects adds entry 90ml and is placed in the 200ml autoclave, and in 150 ℃ of reaction 10hr, cooled and filtered obtains filter cake 6.2g, percent hydrolysis 65.6% under 0.2MPa.With the filter cake n-hexane extraction,, obtain the 3.3g product with the normal hexane rotary evaporation.It is 5 * 10 that in the filtrate and back is added starting point concentration
6In the 10L micro algae culturing liquid that individual cell is every milliliter, photo-biological reactor is the pipeline reactor that hard silicon boron glass is made, and uniform distribution 2-4 props up the 30W fluorescent tube bulb around the reactor, and water bath with thermostatic control is controlled at 25-30 ℃, feeds to contain 3wt%CO
2Air Mixture, gas flow are 200ml/min.Micro algae growth is rapid, cultivates that density is 1.77g/L after 7 days, obtains algae powder 17.7g after collecting drying.
Embodiment 6
The bead algae powder 18g that collects adds entry 90ml and is placed in the 200ml autoclave, and in 100 ℃ of reaction 10hr, cooled and filtered obtains filter cake 7.2g, percent hydrolysis 60.0% under 0.5MPa.With the filter cake n-hexane extraction,, obtain the 1.80g product with the normal hexane rotary evaporation.It is 5 * 10 that in the filtrate and back is added starting point concentration
6In the 10L micro algae culturing liquid that individual cell is every milliliter, photo-biological reactor is the pipeline reactor that hard silicon boron glass is made, and uniform distribution 2-4 props up the 30W fluorescent tube bulb around the reactor, and water bath with thermostatic control is controlled at 25-30 ℃, feeds to contain 3wt%CO
2Air Mixture, gas flow are 200ml/min.Micro algae growth is rapid, cultivates that density is 1.67g/L after 7 days, obtains algae powder 16.7g after collecting drying.
Embodiment 7
The golden algae powder 18g that collects, the hydrochloric acid soln 90ml that adds 5% (weight) is placed in the 200ml autoclave, and in 150 ℃ of reaction 10hr, cooled and filtered obtains filter cake 5.5g, percent hydrolysis 69.4% under 1MPa.With the filter cake n-hexane extraction,, obtain the 3.4g product with the normal hexane rotary evaporation.It is 5 * 10 that in the filtrate and back is added starting point concentration
6In the 10L micro algae culturing liquid that individual cell is every milliliter, photo-biological reactor is the pipeline reactor that hard silicon boron glass is made, and uniform distribution 2-4 props up the 30W fluorescent tube bulb around the reactor, and water bath with thermostatic control is controlled at 25-30 ℃, feeds to contain 3wt%CO
2Air Mixture, gas flow are 200ml/min.Micro algae growth is rapid, cultivates that density is 1.81g/L after 7 days, obtains algae powder 18.1g after collecting drying.
Embodiment 8
The golden algae powder 18g that collects, the sulphuric acid soln 90ml that adds 10% (weight) is placed in the 200ml autoclave, and in 150 ℃ of reaction 10hr, cooled and filtered obtains filter cake 6.5g, percent hydrolysis 63.9% under 3MPa.With the filter cake n-hexane extraction,, obtain the 3.4g product with the normal hexane rotary evaporation.It is 5 * 10 that in the filtrate and back is added starting point concentration
6In the 10L micro algae culturing liquid that individual cell is every milliliter, photo-biological reactor is the pipeline reactor that hard silicon boron glass is made, and uniform distribution 2-4 props up the 30W fluorescent tube bulb around the reactor, and water bath with thermostatic control is controlled at 25-30 ℃, feeds to contain 3wt%CO
2Air Mixture, gas flow are 200ml/min.Micro algae growth is rapid, cultivates that density is 1.64g/L after 7 days, obtains algae powder 16.4g after collecting drying.
Embodiment 9
The golden algae powder 18g that collects, the sodium hydroxide solution 90ml that adds 10% (weight) is placed in the 200ml autoclave, and in 150 ℃ of reaction 10hr, cooled and filtered obtains filter cake 10.5g, percent hydrolysis 41.6% under 0.1MPa.With the filter cake n-hexane extraction,, obtain the 2.4g product with the normal hexane rotary evaporation.It is 5 * 10 that in the filtrate and back is added starting point concentration
6In the 10L micro algae culturing liquid that individual cell is every milliliter, photo-biological reactor is the box reactor of plate that hard silicon boron glass is made, and uniform distribution 2-4 props up the 30W fluorescent tube bulb around the reactor, and water bath with thermostatic control is controlled at 25-30 ℃, feeds to contain 3wt%CO
2Air Mixture, gas flow are 200ml/min.Micro algae growth is rapid, cultivates that density is 1.02g/L after 7 days, obtains algae powder 10.2g after collecting drying.
Embodiment 10
The golden algae powder 18g that collects adds entry 90ml and is placed in the 200ml autoclave, and in 100 ℃ of reaction 10hr, cooled and filtered obtains filter cake 16.7g, percent hydrolysis 7.2% under 4MPa.With the filter cake n-hexane extraction,, obtain the 0.9g product with the normal hexane rotary evaporation.It is 5 * 10 that in the filtrate and back is added starting point concentration
6In the 10L micro algae culturing liquid that individual cell is every milliliter, photo-biological reactor is the pipeline reactor that hard silicon boron glass is made, and uniform distribution 2-4 props up the 30W fluorescent tube bulb around the reactor, and water bath with thermostatic control is controlled at 25-30 ℃, feeds to contain 3wt%CO
2Air Mixture, gas flow are 200ml/min.Cultivate that density is 0.12g/L after 7 days, obtain algae powder 1.2g after collecting drying.
Embodiment 11
The golden algae powder 18g that collects adds entry 90ml and is placed in the 200ml autoclave, and in 250 ℃ of reaction 10hr, cooled and filtered obtains filter cake 4.2g, percent hydrolysis 76.7% under 1MPa.With the filter cake n-hexane extraction,, obtain the 3.6g product with the normal hexane rotary evaporation.It is 5 * 10 that in the filtrate and back is added starting point concentration
6In the 10L micro algae culturing liquid that individual cell is every milliliter, photo-biological reactor is the pipeline reactor that hard silicon boron glass is made, and uniform distribution 2-4 props up the 30W fluorescent tube bulb around the reactor, and water bath with thermostatic control is controlled at 25-30 ℃, feeds to contain 3wt%CO
2Air Mixture, gas flow are 200ml/min.Micro algae growth is rapid, cultivates that density is 1.77g/L after 7 days, obtains algae powder 17.7g after collecting drying.
Embodiment 12
The wet chrysophyceae algae mud 54g that collects, wherein water content 67%, and containing the dry algae powder amount is 18g, adds the hydrochloric acid soln 64ml of 7% (weight), and 100 ℃ of following reflux 2hr of normal pressure and temperature filter, and obtain filter cake 6.3g, percent hydrolysis 65%.Filter cake is obtained extract 3.3g with n-hexane extraction, for showing slightly the bio oil of black.It is 5 * 10 that filtrate is added starting point concentration with in 10% (weight) NaOH aqueous solution and back
6In the 10L micro algae culturing liquid that individual cell is every milliliter, photo-biological reactor is the box reactor of plate that hard silicon boron glass is made, and uniform distribution 2-4 props up the 30W fluorescent tube bulb around the reactor, and water bath with thermostatic control is controlled at 25-30 ℃, feeds to contain 3wt%CO
2Air Mixture, gas flow are 200ml/min.Micro algae growth is rapid, cultivates that density is 4 * 10 after 3 days
7Every milliliter in individual cell, density is 1.77g/L after 7 days, obtains algae powder 17.7g after the collection drying.
Claims (10)
1. the method for little algae cyclic culture and co-producing bio-oil said method comprising the steps of:
1) utilizes bioreactor to carry out the breed of little algae, per generation is cultured little algae of collecting acquisition hydrolyzed solution that under the pressure of 50 ℃-250 ℃ temperature and 0.1MPa-4.0MPa, is hydrolyzed;
2) hydrolyzed solution that step 1) is obtained obtains water and oil phase by separation, and described water joins as nutritive medium that the breeding for little algae provides nutrition in the both culturing microalgae of future generation system, and oil phase is as product;
Wherein in the cyclic culture process of little algae, do not need additionally to add nutrition.
2. method according to claim 1 is characterized in that described little algae is marine microalgae or freshwater microalgae.
3. method according to claim 1 is characterized in that described bioreactor is the box reactor of duct type or plate, and pump circulation or gas lift circulation are adopted in the circulation of little algae nutrient solution.
4. method according to claim 1 is characterized in that light source that described bioreactor adopts is luminescent lamp, fluorescent lamp, sunlight or their are mixed, are used alternatingly.
5. method according to claim 1 is characterized in that the little algae that will collect is processed into dried microalgae powder or the form of little algae that wets before hydrolysis.
6. method according to claim 1, the method for hydrolysis that it is characterized in that described little algae are acid hydrolysis, the neutrallty condition hydrolysis under the High Temperature High Pressure or the acid hydrolysis under the High Temperature High Pressure under the normal pressure.
7. method according to claim 1, the separation method that it is characterized in that described water and oil phase is separatory, filtration or extraction.
8. method according to claim 6 is characterized in that acid used in the described acid hydrolysis is one or more in hydrochloric acid, sulfuric acid, the phosphoric acid, and concentration is 0.1mol/L-2mol/L.
9. method according to claim 6, the condition that it is characterized in that described High Temperature High Pressure are 100 ℃-250 ℃ temperature and the pressure of 0.1MPa-4.0MPa.
10. method according to claim 6, it is characterized in that described neutrallty condition be hydrolyzed in water or the diluted alkaline of 5-10% in hydrolysis.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105661196A CN103215188A (en) | 2012-01-20 | 2012-12-24 | Method for microalgae breeding and biological oil co-production |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210019070 | 2012-01-20 | ||
| CN201210019070.2 | 2012-01-20 | ||
| CN2012105661196A CN103215188A (en) | 2012-01-20 | 2012-12-24 | Method for microalgae breeding and biological oil co-production |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104561153A (en) * | 2015-01-22 | 2015-04-29 | 贵州华清科维环境能源有限责任公司 | Method for producing bio-oil by cultivating algae recycling vinasse nutrients |
| CN106434778A (en) * | 2016-12-05 | 2017-02-22 | 新奥科技发展有限公司 | Method for producing grease from microalgae |
| CN106754386A (en) * | 2016-12-05 | 2017-05-31 | 新奥科技发展有限公司 | A kind of both culturing microalgae method |
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| CN101611125A (en) * | 2006-09-14 | 2009-12-23 | 生物燃料箱公司 | Cellular lipids is to the method for conversion fully and efficiently of biofuel |
| CN102229895A (en) * | 2011-06-14 | 2011-11-02 | 重庆工商大学 | Method for producing organic nitrogen source of yeast culture medium by using water-bloom microalgae biomasses |
| CN102311921A (en) * | 2010-07-07 | 2012-01-11 | 中国石油化工股份有限公司 | Method for culturing chlorella |
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| CN101368193B (en) * | 2008-10-14 | 2010-12-22 | 蔡志武 | Process for preparing fine algae cultivation coupling biological diesel oil refining |
| WO2010045631A2 (en) * | 2008-10-17 | 2010-04-22 | Stc.Unm | Method and unit for large-scale algal biomass production |
| CN101423769A (en) * | 2008-11-16 | 2009-05-06 | 中国海洋大学 | Method for preparing biodiesel based on marine microalgal |
| CN101429467B (en) * | 2008-12-24 | 2010-10-27 | 青岛生物能源与过程研究所 | Method for simultaneously extracting lipid and protein from microalgae |
| CN102071038A (en) * | 2009-11-20 | 2011-05-25 | 田原宇 | Microalgae high-pressure continuous tower-type liquefying process |
| CN102295983A (en) * | 2011-07-21 | 2011-12-28 | 上海辰浩生物技术有限公司 | Production method for comprehensive utilization of microalgae |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101611125A (en) * | 2006-09-14 | 2009-12-23 | 生物燃料箱公司 | Cellular lipids is to the method for conversion fully and efficiently of biofuel |
| CN102311921A (en) * | 2010-07-07 | 2012-01-11 | 中国石油化工股份有限公司 | Method for culturing chlorella |
| CN102229895A (en) * | 2011-06-14 | 2011-11-02 | 重庆工商大学 | Method for producing organic nitrogen source of yeast culture medium by using water-bloom microalgae biomasses |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104561153A (en) * | 2015-01-22 | 2015-04-29 | 贵州华清科维环境能源有限责任公司 | Method for producing bio-oil by cultivating algae recycling vinasse nutrients |
| CN104561153B (en) * | 2015-01-22 | 2019-07-19 | 贵州华清科维环境能源有限责任公司 | A method of it recycling vinasse nutrient culture algae and carries out bio-oil production |
| CN106434778A (en) * | 2016-12-05 | 2017-02-22 | 新奥科技发展有限公司 | Method for producing grease from microalgae |
| CN106754386A (en) * | 2016-12-05 | 2017-05-31 | 新奥科技发展有限公司 | A kind of both culturing microalgae method |
| CN106434778B (en) * | 2016-12-05 | 2019-09-20 | 新奥科技发展有限公司 | A kind of method of microalgae production grease |
| CN106754386B (en) * | 2016-12-05 | 2019-12-03 | 新奥科技发展有限公司 | A kind of both culturing microalgae method |
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| WO2013107248A1 (en) | 2013-07-25 |
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