CN103211581A - Temperature-and-illumination-controllable type retinal photo-injury quantitative research device - Google Patents
Temperature-and-illumination-controllable type retinal photo-injury quantitative research device Download PDFInfo
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- CN103211581A CN103211581A CN2013101383641A CN201310138364A CN103211581A CN 103211581 A CN103211581 A CN 103211581A CN 2013101383641 A CN2013101383641 A CN 2013101383641A CN 201310138364 A CN201310138364 A CN 201310138364A CN 103211581 A CN103211581 A CN 103211581A
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Abstract
The invention relates to a retinal photo-injury quantitative research device, in particular to a temperature-and-illumination-controllable type retinal photo-injury quantitative research device. The research device comprises a camera obscura, a light-emitting diode (LED) face light source, a light source driving unit, a semiconductor refrigeration sheet, a refrigeration sheet driving unit, a photo-sensor, a temperature sensor and a control unit. The light source driving unit drives the LED face light source, the refrigeration sheet driving unit drives the semiconductor refrigeration sheet, the LED face light source is arranged in the camera obscura, and the semiconductor refrigeration sheet, the photo-sensor and the temperature sensor are arranged in a test area. By means of the face light source, the semiconductor refrigeration sheet, the photo-sensor, the temperature sensor and a control unit with a single chip mirco-processor serving as a core, the research device can adjust and control a light strength and temperature device in a sealed space, achieves double control and quantitative monitoring of light strength and temperature, and is fast in reaction, stable in result, high in precision and particularly suitable for photo-injury test research at an isolated cell level.
Description
Technical field
The present invention relates to a kind of retina photodamage quantitative study device, specifically a kind of temperature and illumination controllable type retina photodamage quantitative study device.
Background technology
Photic damage, promptly strong excessively light or look at the infringement that light source causes retina for a long time straight.The instrument that is applied to quantitatively or the photic damage of qualitative investigation retina cell is studied is called the photic damage research device.The photic damage research device generally includes a confined space, and a luminous body and a light intensity control device etc. are by regulating the brightness of luminous body, to the purpose that cell in the confined space or laboratory animal carry out in short-term, intensive illumination reaches photic damage.Yet, aspect the photic damage experimentation, still do not have unified stable experimental facilities both at home and abroad and control the temperature and the illumination of experimental situation, and the simple instrument of taking self to carry to carry out more at present, the efficient of its experiment, repeatability and credibility are all lower.Simultaneously, along with photic damage is tested by qualitative to quantitative development trend.Traditional point source is because its inherent limitation is difficult to reach the light intensity of homogeneous, and exists and be difficult to realize the digitized regulation and control, and problems such as monitoring in real time can't satisfy the experiment demand of research that light is hindered in some occasion.Publishing in 2012 during relative photo damage that the e38690 page or leaf of PLoS One delivers studies at the Int J Ophthalmol 151-157 page or leaf of publishing in 2012 and Ueki Y etc. as Feng M etc., the mode that employing is placed the fluorescent lamp of one or more certain wavelength in inside, darkroom is carried out the research that photic damage is tested, cell is accepted the uniformity of illumination in can't guaranteeing to test, and can't formulate unified, believable standard to the evaluation of experiment.And in the photic damage research device, temperature is as the significant variable of photic damage research, is left in the basket in the research of quite a few Chinese scholars, and its stability and repeatability that may cause testing greatly reduces.
Summary of the invention
The objective of the invention is to provide a kind of temperature and illumination controllable type retina photodamage quantitative study device in order to overcome the deficiencies in the prior art, to realize that the problem of light intensity stable homogeneous regulation and control in a certain plane is applicable to the quantitative temperature adjusting requirement of cell injury experiment simultaneously.
For achieving the above object, the invention discloses a kind of temperature and illumination controllable type retina photodamage quantitative study device, it is characterized in that: comprise camera bellows, the LED area source, light source driving units, semiconductor chilling plate, the cooling piece driver element, optical sensor, temperature sensor and control unit, described light source driving units driving LED area source, cooling piece drive unit drives semiconductor chilling plate, described LED area source is arranged in the camera bellows and the direct projection Experimental Area, described semiconductor chilling plate, optical sensor and temperature sensor are arranged in the Experimental Area, described control unit is that core is provided with the first input end and second input with the chip microprocessor, the output of described optical sensor and temperature sensor is connected with second input with the control unit first input end respectively, described control unit also is provided with first outfan and is connected with light source driving units with the cooling piece driver element respectively with second outfan, described control unit is provided with real-time luminosity value memorizer, the brightness settings memorizer, real time temperature value memorizer, desired temperature memorizer and comparator, described comparator compares the numerical value in real-time luminosity value memorizer and brightness settings memorizer and real time temperature value memorizer and the desired temperature memorizer, and described control unit is controlled LED area source and semiconductor chilling plate according to comparative result by first outfan and second outfan.
Compared with prior art, the present invention is by being provided with area source, conductor cooling piece, optical sensor, temperature sensor and being the control core unit with the chip microprocessor, can digitized light intensity and temperature device in the regulation and control confined space, dual control and quantitative monitoring have been realized to temperature and light intensity, reaction is fast, the result is stable, precision is high, is particularly useful for the photic damage experimentation on the isolated cells level.
The present invention is further described below in conjunction with drawings and Examples.
Description of drawings
Fig. 1 is the structure perspective view of the specific embodiment of the invention;
Fig. 2 is a specific embodiment of the invention electricity theory diagram.
The specific embodiment
Shown in 1 figure, temperature and illumination controllable type retina photodamage quantitative study device comprise camera bellows 1, LED area source 2, light source driving units, semiconductor chilling plate 3, cooling piece driver element, optical sensor 4, temperature sensor 5 and control unit, described light source driving units driving LED area source 2, cooling piece drive unit drives semiconductor chilling plate 3.The shape of camera bellows 1 can be as required, camera bellows 1 is a cuboid in this specific embodiment, described LED area source 2 matches with camera bellows 1 intracavity bottom shape and is installed in the bottom, described LED area source 2 tops constitute the Experimental Area, described LED area source 2 tops are provided with culture dish shelf 6, described culture dish shelf 6 be arranged in parallel with LED area source 2, described LED area source 2 direct projection Experimental Areas, like this can be so that the Experimental Area beam intensity ratio is more even, culture dish shelf 6 adopts high clear sheet to make, in order in time to detect light intensity and temperature, described optical sensor 4 and temperature sensor 5 are arranged on the culture dish shelf 6, in order to detect more exactly, a plurality of optical sensors 4 and temperature sensor 5 can be set in this device, this is evenly distributed on the Experimental Area with it, carries out zoopery for convenience, can also be provided with animal holder 7 in described Experimental Area.In this specific embodiment, light intensity sensor is selected for use and is TSL2561, an inner light sensitive diode (passage 0) and an infrared response light sensitive diode (passage 1) and two the integrated integral a/d converters of connecting of this light intensity sensor, photosensitive current conversion can be become a numeral output, have the ability that nearly photopic response in 20 dynamic ranges is provided.LM35 is selected in temperature sensor 5 for use, and these temperature sensor 5 output voltages are directly proportional with Celsius temperature, and the linearity is good, and is highly sensitive, moderate accuracy, and its output sensitivity is 10.0MV/ ℃, and precision reaches 0.5 ℃, and its measuring range is-55---150 ℃.Self-heating effect low (0.08 ℃) in static temperature, the running voltage broad, can be 4---operate as normal in the power supply voltage range of 20V, and power consumption is economic, and operating current is generally less than 60uA, and output impedance is low, is 0.1 Ω when the 1MA load.According to the output characteristics of LM35 as can be known, when temperature 0---between 150 ℃ during conversion, the voltage of its outfan correspondence is 0---150V, this voltage is delivered to 3 half word display gauge heads detection signal input of (being made up of ICL7107 and relevant circuit) after potentiometer W3 dividing potential drop, when the voltage of input input is 150V, making the numerical value of demonstration by regulator potentiometer is 150.0, and the numerical value that the digital display gauge outfit shows after adjusting is exactly the temperature value of actual measurement.Described semiconductor chilling plate 3 is arranged on the sidewall of culture dish shelf 6 tops, and semiconductor chilling plate 3 heats refrigeration system with tradition and compares, and has volume and urinates in carrying, put the accurately rapid and advantages of environment protection of temperature control, temperature control.Described control unit is arranged on the below of camera bellows 1 inner chamber LED area source 2, as shown in Figure 2, described control unit is a core with arm processor STM32F103, be provided with the first input end and second input, the output of described optical sensor 4 and temperature sensor 5 is connected with second input with the control unit first input end respectively, described control unit is provided with also, and first outfan is connected with light source driving units with the cooling piece driver element respectively with second outfan, described control unit is provided with real-time luminosity value memorizer, the brightness settings memorizer, real time temperature value memorizer, desired temperature memorizer and comparator, described comparator compares the numerical value in real-time luminosity value memorizer and brightness settings memorizer and real time temperature value memorizer and the desired temperature memorizer, described control unit is controlled LED area source 2 and semiconductor chilling plate 3 according to comparative result by first outfan and second outfan, real-time luminosity value memorizer wherein, the brightness settings memorizer, real time temperature value memorizer and desired temperature memorizer have internal storage to serve as, and comparator is realized by internal processes.Light source driving units control LED area source 2 adopts pulse width modulation modes, and LED connects with inductance, and in parallel with fly-wheel diode, when the derailing switch conducting, power supply is powered to led light source through inductance like this, the inductance coil energy storage; When derailing switch ended, the power supply breaking circuit was powered to led light source by inductance coil, discharged energy stored, thereby made that the electric current of process led light source is still to keep brightness stability in the derailing switch cutout.In order to understand the experiment situation more easily, this device preferably also is provided with display screen 8, and described display screen 8 is connected with control unit, is used for showing the real-time luminosity value and the temperature value of Experimental Area.The display screen 8 preferred touch screens that adopt can be used for man-machine interaction simultaneously and carry out data query and setting.The programming of comparator, light source driving units control and the control of cooling piece driver element is prior art, repeats no more herein.
In research to the retina cell photic damage, whether need under the clear and definite equal conditions illumination relevant with the radiation wave band of illumination to the order of severity of retina cell damage, therefore the led light source in the described LED area source 2 is selected the LED lamp of different colours as required for use.Consider the integrity that will keep equipment, if use the LED lamp of single wave band then change very inconvenience.In order to adapt to different radiation wave band easily to the retina cell Study on Damage, because the light that white light emitting diode (white light LEDs) sends has comprised visible light, near-infrared band and nearultraviolet rays wave band, brightness is high, this installs described LED area source 2 and selects white led lamps for use, and the position near LED area source 2 is provided with elongated slot seam 9 on described camera bellows 1 sidewall, described camera bellows 1 inwall and elongated slot stitch 9 relative positions and are provided with guide groove, described elongated slot seam 9 and the cooperation of guide groove intermediate gap are inserted with optical filter 10, optical filter 10 is parallel with LED area source 2, can utilize optical filter 10 these optics to choose required radiation wave band like this.Such as inserting blue color filter 10, the light that white light LEDs sends is by blue color filter 10, ejaculation be a branch of blue light, and green glow, HONGGUANG are few, have most ofly been absorbed by optical filter 10.Be provided with by this structure, reach under the situation of not changing LED, realize the illumination of different-waveband under the identical light intensity quantitative study to the damage of retina cell.
Claims (9)
1. temperature and illumination controllable type retina photodamage quantitative study device, it is characterized in that: comprise camera bellows, the LED area source, light source driving units, semiconductor chilling plate, the cooling piece driver element, optical sensor, temperature sensor and control unit, described light source driving units driving LED area source, cooling piece drive unit drives semiconductor chilling plate, described LED area source is arranged in the camera bellows and the direct projection Experimental Area, described semiconductor chilling plate, optical sensor and temperature sensor are arranged in the Experimental Area, described control unit is that core is provided with the first input end and second input with the chip microprocessor, the output of described optical sensor and temperature sensor is connected with second input with the control unit first input end respectively, described control unit is provided with also, and first outfan is connected with light source driving units with the cooling piece driver element respectively with second outfan, described control unit is provided with real-time luminosity value memorizer, the brightness settings memorizer, real time temperature value memorizer, desired temperature memorizer and comparator, described comparator compares the numerical value in real-time luminosity value memorizer and brightness settings memorizer and real time temperature value memorizer and the desired temperature memorizer, and described control unit is controlled LED area source and semiconductor chilling plate according to comparative result by first outfan and second outfan.
2. temperature according to claim 1 and illumination controllable type retina photodamage quantitative study device, it is characterized in that: described camera bellows is a cuboid, described LED area source and camera bellows intracavity bottom shape match and are installed in the bottom, described LED area source top is provided with the culture dish shelf, described culture dish shelf and LED area source be arranged in parallel, described optical sensor and temperature sensor are arranged on the culture dish shelf, and described semiconductor chilling plate is arranged on the sidewall of culture dish shelf top.
3. temperature according to claim 2 and illumination controllable type retina photodamage quantitative study device, it is characterized in that: described camera bellows is a cuboid, described LED area source and camera bellows intracavity bottom shape match and are installed in the bottom, described LED area source top is provided with the culture dish shelf, described culture dish shelf and LED area source be arranged in parallel, and described semiconductor chilling plate is arranged on the sidewall of culture dish shelf top.
4. temperature according to claim 1 and illumination controllable type retina photodamage quantitative study device, it is characterized in that: be provided with a plurality of optical sensors and temperature sensor, described optical sensor and temperature sensor are evenly distributed on the Experimental Area.
5. temperature according to claim 1 and illumination controllable type retina photodamage quantitative study device, it is characterized in that: described light source driving units arteries and veins adopts wide modulation system.
6. temperature according to claim 1 and illumination controllable type retina photodamage quantitative study device is characterized in that: be provided with animal holder in the described Experimental Area.
7. temperature according to claim 1 and illumination controllable type retina photodamage quantitative study device, it is characterized in that: also be provided with display screen, described display screen is connected with control unit, is used for showing the real-time luminosity value and the temperature value of Experimental Area.
8. temperature according to claim 7 and illumination controllable type retina photodamage quantitative study device is characterized in that: described display screen adopts touch screen, is used for man-machine interaction simultaneously.
9. temperature according to claim 1 and illumination controllable type retina photodamage quantitative study device, it is characterized in that: described LED area source is selected white led lamps for use, position near the LED area source on the described camera bellows sidewall is provided with the elongated slot seam, the position that described camera bellows inwall is relative with the elongated slot seam is provided with guide groove, and described elongated slot seam and the cooperation of guide groove intermediate gap are inserted with optical filter.
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| CN103876840A (en) * | 2013-12-20 | 2014-06-25 | 江苏省人民医院 | Optical-damage experimental device |
| CN105675609A (en) * | 2016-01-19 | 2016-06-15 | 北京阳明智道光电科技有限公司 | Measuring device and quantitative assessment method of retinal photic injury |
| CN107158361A (en) * | 2017-05-25 | 2017-09-15 | 温州医科大学 | Application of the REG1A albumen in treatment and/or prevention retinal cell apoptosis medicine is prepared |
| CN113412792A (en) * | 2021-07-12 | 2021-09-21 | 温州眼视光国际创新中心 | Myopia animal model construction instrument |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103876840A (en) * | 2013-12-20 | 2014-06-25 | 江苏省人民医院 | Optical-damage experimental device |
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| CN105675609B (en) * | 2016-01-19 | 2018-03-20 | 北京阳明智道光电科技有限公司 | A kind of retinal light damage measurement apparatus and quantitative evaluation method |
| CN107158361A (en) * | 2017-05-25 | 2017-09-15 | 温州医科大学 | Application of the REG1A albumen in treatment and/or prevention retinal cell apoptosis medicine is prepared |
| CN107158361B (en) * | 2017-05-25 | 2019-03-12 | 温州医科大学 | Application of the REG1A albumen in preparation treatment and/or prevention retinal cell apoptosis drug |
| CN113412792A (en) * | 2021-07-12 | 2021-09-21 | 温州眼视光国际创新中心 | Myopia animal model construction instrument |
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