CN103199714B - Power source device of electrophoresis system - Google Patents
Power source device of electrophoresis system Download PDFInfo
- Publication number
- CN103199714B CN103199714B CN201310133053.6A CN201310133053A CN103199714B CN 103199714 B CN103199714 B CN 103199714B CN 201310133053 A CN201310133053 A CN 201310133053A CN 103199714 B CN103199714 B CN 103199714B
- Authority
- CN
- China
- Prior art keywords
- voltage
- unit
- output
- electrophoresis system
- circuit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Dc-Dc Converters (AREA)
Abstract
The invention relates to a power source device of an electrophoresis system. The power source device of the electrophoresis system comprises an input unit, a pre-voltage-stabilization unit, a switch transduction unit and a high-voltage transformation output unit, wherein the input unit, the pre-voltage-stabilization unit, the switch transduction unit and the high-voltage transformation output unit are connected in sequence. Grid power frequency alternating voltage is converted into a programmable error-corrected direct current power source firstly, then the direct current power source is converted into high voltage pulse signals, and then the high voltage pulse signals are converted into high voltage direct voltage and a high voltage direct current, wherein the high voltage direct voltage and the high voltage direct current are needed by the electrophoresis system. The power source device of the electrophoresis system is characterized in that the pre-voltage-stabilization unit, the switch transduction unit and the high-voltage transformation output unit and a control unit construct closed loop feedback control. The closed loop feedback control can conduct self-adaption regulations according to power source requirements of the electrophoresis system, load changes of a buffer solution and output error analysis results of the high-voltage transformation output unit, and supply the stable high voltage direct voltage and the stable high voltage direct current for the electrophoresis system in a working method of constant voltage or constant current or constant power. The power source device of the electrophoresis system can be used for an electrophoresis system which is high in requirement for power source stability and can enable a potential of hydrogen (pH) gradient of the buffer solution in the electrophoresis system to be more stable.
Description
Technical field
The present invention relates to a kind of supply unit, particularly about a kind of supply unit that can be used in large-scale Liquid isoelectric focusing electrophoresis system.
Background technology
In recent years, Liquid isoelectric focusing electrophoretic techniques is filled with new vitality to the analysis of large biological molecule with being separated.As protein analysis be separated in, the loss of protein can be reduced to minimum by Liquid isoelectric focusing electrophoretic techniques, can be competent at comparatively meticulous protein analysis work, and complete protein preparative separation experiment.In Liquid isoelectric focusing electrophoresis system, the stability of pH of buffer gradient is the key factor affecting Separation of Proteins quality always.In order to ensure the stability of pH of buffer gradient, people set about from the mode of filling buffer solution: wherein, a kind of mode directly the mixture solution with the different ampholytes of lower electrophoretic mobility is injected disengagement chamber, under the effect of electric field, the pH gradient that progressively formation one is natural; Another kind of mode is, at different buffer solution inlets, the parallel multiple buffer solution injecting different pH simultaneously, forms an artificial pH gradient.Above-mentioned two kinds of modes, the continuous filling of buffer solution and the uninterrupted loading of sample, all can cause the conductance being separated electric field arrival end unstable, thus affect the separation accuracy of electrophoresis.
When Separation of Proteins, in Liquid isoelectric focusing electrophoresis system, the pH gradient of buffer solution is maintained by electric field, and stable pH gradient depends on the adaptive load ability of system power supply.Existing Liquid isoelectric focusing electrophoresis system generally uses traditional " Constant power supply ", and namely user in use, and " Constant power supply " once can only export the one in constant voltage, constant current or invariable power.If " Constant power supply " can adjust and then keep the settings of voltage or electric current adaptively, so just can extend the stabilization time of disengagement chamber front end pH gradient, therefore, development possesses the controllable high-voltage stabilized power supply of adaptive ability, to ensure the stability of pH of buffer gradient in free stream cataphoresis system better, it is the general trend of current research.
Summary of the invention
For the problems referred to above, the object of this invention is to provide one can as required, with the working method of constant voltage, constant current or invariable power, provides the supply unit of the electrophoresis system of stable high-voltage DC power supply adaptively.
For achieving the above object, the present invention takes following technical scheme: a kind of supply unit of electrophoresis system, is characterized in that: it comprises input unit, pre-voltage regulation unit, switch transducing unit, high pressure conversion output unit, control unit and Auxiliary Power Units, input unit input connects electrical network, and output connects pre-voltage regulation unit and Auxiliary Power Units input respectively, cuts off the input of electric network source in time there are abnormal conditions, pre-voltage regulation unit output connecting valve transducing unit input, the power frequency ac voltage provided by input unit is converted to direct current and exports, and exports to switch transducing unit, is also exported by direct current simultaneously and isolates with electrical network, switch transducing unit output connects high pressure conversion output unit input, and the direct current provided by pre-voltage regulation unit exports and is converted to high-voltage pulse signal, exports to high pressure conversion output unit, high pressure conversion output unit output connects electrophoresis system disengagement chamber electrode, the high-voltage pulse signal that switch transducing unit provides is converted to high-voltage dc voltage, electric current that electrophoresis system can use, control unit connects host computer by data/address bus, data interaction is carried out with host computer, high pressure conversion output unit output is connected by data/address bus, gather the high-voltage dc voltage that high pressure conversion output unit exports, electric current, pre-voltage regulation unit control end is connected by data/address bus, according to the power requirement of electrophoresis system and the change of buffer solution load, and high pressure conversion output unit output error analysis result, the direct current regulating and controlling pre-voltage regulation unit exports, by data/address bus connecting valve transducing unit controls end, the reverse frequency of the high-voltage pulse signal that regulating switch transducing unit exports and duty ratio, connect high pressure conversion output unit control end by data/address bus, open according to the control command of host computer or turn off the output of high pressure conversion output unit, Auxiliary Power Units output connection control unit, for it provides operating voltage.
Above-mentioned pre-voltage regulation unit is made up of the high-frequency isolation transformer of connecting successively, surge protecting circuit, electromagnetic compatibility and Electromagnetic interference filter, full-wave rectifier filter circuit, electric current and voltage temperature current foldback circuit and three permanent feedback control circuits, three permanent feedback control circuits comprise voltage, electric current, power three FEEDBACK CONTROL branches, a direct current power control module is included in each FEEDBACK CONTROL branch, one output feedback ontrol module, one operational amplifier and a gate controlled switch, the input connection control unit of direct current power control module, output passes through the input of high_voltage isolation driver module and D/A converter module concatenation operation amplifier, the input connection control unit of output feedback ontrol module, output passes through the input of high_voltage isolation driver module and D/A converter module concatenation operation amplifier, the output of operational amplifier connects gate controlled switch, three gate controlled switches are with the logical course conducting of one-out-three.
Above-mentioned input unit is made up of the A.C. contactor of connecting successively, earth leakage protective device and fuse protector.
Above-mentioned switch transducing unit comprises full-bridge phase shifting switch control module and have employed the full-bridge high-frequency switching circuit of soft switch technique, the input connection control unit of full-bridge phase shifting switch control module, output connects full-bridge high-frequency switching circuit through a high_voltage isolation driver module, the input of full-bridge high-frequency switching circuit connects the output of pre-voltage regulation unit, and output connects the input of high pressure conversion output unit.
Above-mentioned high pressure conversion output unit is by the high pressure full-wave rectifying circuit of connecting successively, high-pressure filter circuit, high pressure EMI filter circuit and voltage, current sampling circuit, and output control circuit is formed; The output of voltage, current sampling circuit is respectively by a voltage sampling signal conditioning module, a current sampling signal conditioning module connection control unit; The control end of output control circuit is by a high_voltage isolation driver module connection control unit, and output connects electrophoresis system disengagement chamber electrode.
Above-mentioned voltage, current sampling signal conditioning module are formed by the isolation light lotus root circuit of connecting and AD Acquisition Circuit.
The present invention is owing to taking above technical scheme, it has the following advantages: 1, the present invention is by arranging a control unit, according to the power requirement of electrophoresis system and the change of buffer solution load, adjust voltage, current value, power rating that in supply unit, pre-voltage regulation unit exports adaptively, can, when the separation of Liquid isoelectric focusing electrophoresis system is combined with the protein sample of dyestuff, the deviation angle of its initial swimming obviously be strengthened.2, the present invention is by arranging a control unit, real-time accurate gathers the actual output of power supply, pass through related operation, be converted into error controling signal, three permanent outputs of FEEDBACK CONTROL supply voltage, electric current, power, thus can effectively reduce power supply output pulsation, improve the stability that power supply exports further, make pH of buffer gradient in electrophoresis system more stable.3, the present invention by a control unit is set and host computer mutual, the range of Liquid isoelectric focusing electrophoresis system power is set by host computer, and return the operating state of power supply in real time, realize program-controlled intelligent operation, in addition, can also open in time as required or the output of break-make power supply, enhance the security performance of supply unit.The present invention may be used for requiring higher electrophoresis system to power good, as Liquid isoelectric focusing electrophoresis system, thus makes pH of buffer gradient in electrophoresis system more stable.
Accompanying drawing explanation
Fig. 1 is composition schematic diagram of the present invention;
Fig. 2 is the composition schematic diagram of input unit of the present invention;
Fig. 3 is the composition schematic diagram of pre-voltage regulation unit of the present invention;
Fig. 4 is the composition schematic diagram of three permanent feedback control circuits of pre-voltage regulation unit of the present invention;
Fig. 5 is the connection diagram of the full-bridge phase shifting switch control module of switch transducing unit of the present invention;
Fig. 6 is the composition schematic diagram of the full-bridge high-frequency switching circuit of switch transducing unit of the present invention;
Fig. 7 is the composition schematic diagram of high pressure conversion output unit of the present invention;
Fig. 8 is the voltage of high pressure conversion output unit of the present invention, the composition schematic diagram of current sampling circuit;
Fig. 9 is the connection diagram of the high_voltage isolation driver module of high pressure conversion output unit of the present invention;
Figure 10 is the power supply output relation schematic diagram of the present invention under constant voltage, constant current, invariable power operating state.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in detail.
As shown in Figure 1, the present invention forms primarily of input unit 10, pre-voltage regulation unit 20, switch transducing unit 30, high pressure conversion output unit 40, control unit 50 and Auxiliary Power Units 60.Wherein:
Input unit 10 input connects electrical network, and output connects pre-voltage regulation unit 20 and Auxiliary Power Units 60 input respectively, cuts off the input of electric network source, play the effect of protection personal safety during for abnormal conditions occur.
Pre-voltage regulation unit 20 output connecting valve transducing unit 30 input, the power frequency ac voltage for being provided by input unit 10 is converted to direct current and exports, and exports to switch transducing unit 30, is also exported by direct current simultaneously and isolates, to reduce electromagnetic interference with electrical network.
Switch transducing unit 30 output connects high pressure conversion output unit 40 input, and the direct current for being provided by pre-voltage regulation unit 20 exports and is converted to high-voltage pulse signal, exports to high pressure conversion output unit 40.
High pressure conversion output unit 40 output connects electrophoresis system disengagement chamber electrode, and the high-voltage pulse signal for being provided by switch transducing unit 30 is converted to high-voltage dc voltage, the electric current that electrophoresis system can use.
Control unit 50 has the input of multiple signal, output, wherein:
1) connect host computer 70 by data/address bus, carry out data interaction with host computer 70, receive the control command of host computer 70, feed back the working condition of supply unit of the present invention;
2) connect high pressure conversion output unit 40 output by data/address bus, gather high-voltage dc voltage, electric current that high pressure conversion output unit 40 exports, to carry out error analysis;
3) pre-voltage regulation unit 20 control end is connected by data/address bus, with according to the power requirement of electrophoresis system and the change of buffer solution load, and high pressure conversion output unit 40 output error analysis result, the direct current regulating and controlling pre-voltage regulation unit 20 exports, and the closed loop feedback realized for the purpose of constant voltage, constant current or invariable power controls;
4) by data/address bus connecting valve transducing unit 30 control end, the reverse frequency of the high-voltage pulse signal that regulating switch transducing unit 30 exports and duty ratio, control to realize voltage stabilizing;
5) connect high pressure conversion output unit 40 control end by data/address bus, open according to the control command of host computer 70 or turn off the output of high pressure conversion output unit 40.
Auxiliary Power Units 60 output connection control unit 50, for control unit 50 provides operating voltage.
The concrete setting of above-mentioned unit is described in detail below in conjunction with accompanying drawing:
As shown in Figure 2, input unit 10 of the present invention can be made up of the A.C. contactor 17 of connecting successively, earth leakage protective device 18 and fuse protector 19.Wherein, the input of A.C. contactor 17 is connected with electrical network output, and the output of fuse protector 19 is connected with the input of pre-voltage regulation unit 20 and Auxiliary Power Units 60 respectively.
As shown in Figure 3, pre-voltage regulation unit 20 of the present invention can be made up of the high-frequency isolation transformer 21 of connecting successively, surge protecting circuit 22, electromagnetic compatibility and Electromagnetic interference filter 23, full-wave rectifier filter circuit 24, electric current and voltage temperature current foldback circuit 25 and three permanent feedback control circuits 26.Wherein, the input of high-frequency isolation transformer 21 is connected with the output of fuse protector 19 in input unit 10.The output of three permanent feedback control circuits 26 is connected with the input of switch transducing unit 30.The alternating voltage of power frequency is converted to pre-steady direct voltage by full-wave rectifier filter circuit 24.
As shown in Figure 4, three permanent feedback control circuits 26 are primarily of voltage, electric current, power three FEEDBACK CONTROL branches are formed, a direct current power control module is included in each FEEDBACK CONTROL branch, one output feedback ontrol module, one operational amplifier and a gate controlled switch, the input connection control unit 50 of direct current power control module, output passes through the input of a high_voltage isolation driver module and a D/A converter module (D/A) concatenation operation amplifier, the input connection control unit 50 of output feedback ontrol module, output passes through the input of a high_voltage isolation driver module and a D/A converter module (D/A) concatenation operation amplifier, the output of operational amplifier connects gate controlled switch.Above-mentioned three gate controlled switches with the logical course conducting of one-out-three, thus make three permanent feedback control circuits 26 can only with the working method output dc voltage of constant voltage, constant current or invariable power, electric current to the switch transducing unit 30 of rear stage.Wherein, constant voltage working method: the direct current power control module in Voltage Feedback control branch is under the voltage that control unit sends arranges instruction, through high_voltage isolation driver module and D/A converter module, output voltage signalization, under the error correction instruction that simultaneously output feedback ontrol module sends at control unit, output voltage error signal, voltage-set signal and the voltage error signal pre-steady direct voltage that acting in conjunction exports in electric current and voltage temperature current foldback circuit 25 after operational amplifier superposition, be adjusted to the stable DC voltage meeting target, be supplied to the switch transducing unit 30 of rear stage.The working method of constant current or invariable power and the working method of constant voltage similar, repeat no more herein.
As shown in Figure 5, switch transducing unit 30 of the present invention can be made up of the full-bridge high-frequency switching circuit 31 and full-bridge phase shifting switch control module 32 that have employed soft switch technique.The input connection control unit 50 of full-bridge phase shifting switch control module 32, output connects full-bridge high-frequency switching circuit 31 through high_voltage isolation driver module, the input of full-bridge high-frequency switching circuit 31 connects the output of pre-voltage regulation unit 20, and output connects the input of high pressure conversion output unit 40.So-called soft switch technique refers to and increases the resonant element such as inductance, electric capacity in circuit, before and after switching process, introduce resonance, to eliminate the overlap of voltage, electric current, reduces switching loss and switching noise.
As shown in Figure 6, have employed the full-bridge high-frequency switching circuit 31 of soft switch technique: as a MOSFET power tube A/C and B/D conducting in turn for switching tube, differ 180 ° of phase angles, but A and D or B and C can not simultaneously conducting, the first conducting of A and B, is called leading-bridge; Conducting after C and D, is called lagging leg.Wherein, leading-bridge easily realizes zero voltage switch ZVS(Zero Voltage Switch), main output inductor participates in resonance, can meet the impulse electricity needs of shunt capacitance C1 and C2 when switching tube A and B turns off; Lagging leg is due in turn off process, the secondary short circuit of high tension transformer 33, the former limit leakage inductance of high tension transformer 33 is only had to participate in resonance, the impulse electricity work of shunt capacitance C3 and C4 can not be completed rapidly, therefore need to increase former limit resonant inductance Lr on the former limit of lagging leg side, to realize zero voltage switch.Due to the increase of former limit resonant inductance Lr, the transformation period of primary current in positive and negative half period can be extended, easily cause the Loss Rate of secondary duty ratio to raise, reduce power-efficient, so former limit resonant inductance Lr value is unsuitable excessive.Zero voltage switch essence utilizes resonant process to the impulse electricity of shunt capacitance, allows a certain brachium pontis mid-point voltage be raised to the magnitude of voltage of input (DC voltage value of also i.e. pre-voltage regulation unit 20 output) fast or to drop to null value.When switching tube A and B of same brachium pontis or C and D is about to connect, diode D1 and D2 of its parallel connection or D3 and D4 connects, and by the both end voltage of corresponding switching tube pincers at 0V, creates conditions for realizing zero voltage switch.In order to make lagging leg realize zero voltage switch better, can at the two ends of lagging leg an in parallel auxiliary resonant net (inductance L a in Fig. 6, electric capacity C5, C6, the circuit that diode D5, D6 are formed), neither disturb major loop, secondary duty-cycle loss rate can be reduced again, improve power-efficient.
High tension transformer 33 is important component parts of full-bridge high-frequency switching circuit 31, and the setting of its parameter should take into full account the parameter value estimating the real transformers such as leakage inductance, winding electric capacity, peak flux density, direct current winding resistance, high-frequency ac resistance, copper loss, iron loss, weight, temperature rise, window utilization factor.Due to high tension transformer 33 power comparatively large (generally more than hundreds of watts of magnitudes), so the full-bridge transformation topology structure being suitable for high power transmission should be selected.Simultaneously because high voltage arc may reach control loop through high-tension transformer distributed capacitance, so also tackle the stronger decoupling quarantine measures of control loop increase.These are prior art, no longer describe in detail herein.
Switching tube A/B and C/D in full-bridge high-frequency switching circuit 31 is driven by full-bridge phase shifting switch control module 32.Full-bridge phase shifting switch control module 32 outputs signal, two switch tube A/B in full-bridge high-frequency switching circuit 31 and C/D are reached through high_voltage isolation driver module, make it with PWM mode alternate conduction and cut-off, thus the direct voltage that pre-voltage regulation unit 20 provides is reverse into high frequency square wave alternating voltage, be sent to the former limit of high tension transformer 33.High tension transformer 33, by transformation of electrical energy, produces the high-voltage pulse signal that can reach several kilovolts at its secondary.Wherein, switching frequency and PWM duty ratio (being also reverse frequency and the duty ratio of high-voltage pulse signal) are set by control unit 50, can reach the voltage stabilizing control effects of output voltage like this.When occurring abnormal, full-bridge phase shifting switch control module 32 can also cut off the output of full-bridge high-frequency switching circuit 31, plays the effect of protective circuit device simultaneously.
As shown in Figure 7, high pressure conversion output circuit 40 of the present invention can by the high pressure full-wave rectifying circuit 41 of connecting successively, high-pressure filter circuit 42, high pressure EMI filter circuit 43 and voltage, current sampling circuit 44, and output control circuit 45 is formed.Wherein, the input of high pressure full-wave rectifying circuit 41 is connected with the output of switch transducing unit 30.The output of output control circuit 45 and electrophoresis system disengagement chamber Electrode connection.The high-voltage pulse signal rectification that switch transducing unit 30 provides by high pressure full-wave rectifying circuit 41 and high-pressure filter circuit 42, filtering, and then convert high-voltage dc voltage to; Wherein, high-pressure filter circuit 42 is made up of output inductor device and electric capacity, and the setting parameter of output inductor device needs to consider that filter circuit exports the many factors such as the maximum of effective current and maximum fluctuation electric current, inductance core, power capacity and filter capacitor.In general, the current pulsation value can choosing output inductor is 20% of maximum output current, and simultaneously when 1/2nd pulsating quantities, output inductor should keep continuous state.High pressure EMI filter circuit 43, for improving the antijamming capability of high-voltage dc voltage further, reduces electromagnetic noise, removes ripple interference.
As shown in Figure 8, the high-voltage dc voltage that voltage, current sampling circuit 44 pairs of hv filtering EMI circuit 43 export, electric current carry out resistance capacitance balance voltage stabilization and current stabilization filtering sampling, and the voltage gathered, current signal send into control unit 50 respectively through a voltage sampling signal conditioning module, a current sampling signal conditioning module.Described voltage, current sampling signal conditioning module all can by the isolation light lotus root circuit of connecting and AD(moduluses) Acquisition Circuit forms.In addition, because the high tension transformer 33 in switch transducing unit 30 easily high-pressure leakage occurs, therefore the preferred Low Drift Temperature precision resistance of the present invention builds voltage, current sampling circuit 44.
As shown in Figure 9, the control end of output control circuit 45 is by a high_voltage isolation driver module connection control unit 50, and under the control of control unit 50, open or turn off high voltage direct current export, also namely power supply of the present invention exports, to ensure electrophoresis system job security.
In above-described embodiment, can adopt if the digital isolating chip ADUM1410 of ADI company is as the high_voltage isolation driver module in Fig. 4, Fig. 5 and Fig. 9, the high-tension circuits such as the pre-voltage regulation unit 20 of control unit 50 and its control, switch transducing unit 30 and high transformation output unit 40 are isolated.
In above-described embodiment, singlechip control chip can be adopted to make control unit 50.
In above-described embodiment, UC3879 main control chip can be adopted to make full-bridge phase shifting switch control module 32.
In above-described embodiment, in supply unit, overvoltage, overcurrent, thermal-shutdown circuit can also be set, and corresponding fault indication signal is fed back to control unit 50.
As shown in Figure 10, be the output of the present invention under constant voltage, constant current, invariable power operating state, its voltage V, electric current I, power P and the time dependent schematic diagram of load R:
When isoelectric focusing electrophoresis, the pH gradient of Separation of Proteins buffer solution is maintained by electric field, and stable pH gradient depends on the adaptive load ability of Liquid isoelectric focusing electrophoresis system stabilized power supply.In electrophoresis process, electrophoresis cavity can be equivalent to a load resistance, its resistance height is determined by the ionic strength of buffer system.At electrophoresis initial time, because the impedance of buffer solution is less, electric current is comparatively large, and when exceeding a certain limit, the Joule heat of generation just may cause system works failure.Therefore need to pre-set upper current limit value I
w, when electric current reaches I
wtime, the present invention starts control unit, with constant current output operation.T0 ~ t1 is exactly the constant current output stage.In the constant current output stage, in buffer system, the trend swimming of ampholytes causes the voltage between iontophoretic electrode to raise and power output increase.According to the exchange capacity of system to Joule heat, under the prerequisite ensureing system worked well, pre-set the upper limit P of power output
w.When power output reaches P
wtime, the present invention starts control unit, with constant power output operation.T1 ~ t2 is exactly the constant power output stage.In the constant power output stage, voltage is still in rising, and electric current is in decline, and the product of voltage and current remains unchanged.Along with continuing to increase of electrophoresis system impedance RL, output voltage continues to rise.Therefore the higher limit U pre-setting voltage is needed
w, when voltage reaches U
wtime, the present invention starts control unit, works with constant voltage output state.It is exactly constant voltage output stage after t2.
The various embodiments described above are only for illustration of the present invention, and wherein the structure, connected mode etc. of each parts all can change to some extent, and every equivalents of carrying out on the basis of technical solution of the present invention and improvement, all should not get rid of outside protection scope of the present invention.
Claims (8)
1. a supply unit for electrophoresis system, is characterized in that: it comprises input unit, pre-voltage regulation unit, switch transducing unit, high pressure conversion output unit, control unit and Auxiliary Power Units;
Described input unit input connects electrical network, and output connects described pre-voltage regulation unit and described Auxiliary Power Units input respectively, cuts off the input of electric network source in time there are abnormal conditions;
Described pre-voltage regulation unit output connects described switch transducing unit input, the power frequency ac voltage that described input unit provides is converted to direct current and exports, export to described switch transducing unit, is also exported by direct current simultaneously and isolates with electrical network;
Described switch transducing unit output connects described high pressure conversion output unit input, and the direct current provided by described pre-voltage regulation unit exports and is converted to high-voltage pulse signal, exports to described high pressure conversion output unit;
Described high pressure conversion output unit output connects electrophoresis system disengagement chamber electrode, the high-voltage pulse signal that described switch transducing unit provides is converted to high-voltage dc voltage, electric current that described electrophoresis system can use;
Described control unit connects host computer by data/address bus, data interaction is carried out with described host computer, described high pressure conversion output unit output is connected by data/address bus, gather the high-voltage dc voltage that described high pressure conversion output unit exports, electric current, described pre-voltage regulation unit control end is connected by data/address bus, according to the power requirement of described electrophoresis system and the change of buffer solution load, and described high pressure conversion output unit output error analysis result, the direct current regulating and controlling described pre-voltage regulation unit exports, described switch transducing unit controls end is connected by data/address bus, regulate and control reverse frequency and the duty ratio of the high-voltage pulse signal that described switch transducing unit exports, connect described high pressure conversion output unit control end by data/address bus, open according to the control command of described host computer or turn off the output of described high pressure conversion output unit,
Described Auxiliary Power Units output connects described control unit, for it provides operating voltage;
Described pre-voltage regulation unit is made up of the high-frequency isolation transformer of connecting successively, surge protecting circuit, electromagnetic compatibility and Electromagnetic interference filter, full-wave rectifier filter circuit, electric current and voltage temperature current foldback circuit and three permanent feedback control circuits, described three permanent feedback control circuits comprise voltage, electric current, power three FEEDBACK CONTROL branches, a direct current power control module is included in FEEDBACK CONTROL branch described in each, one output feedback ontrol module, one operational amplifier and a gate controlled switch, the input of described direct current power control module connects described control unit, output is connected the first input end of described operational amplifier with D/A converter module by high_voltage isolation driver module, the input of described output feedback ontrol module connects described control unit, output is connected the first input end of described operational amplifier with D/A converter module by high_voltage isolation driver module, the output of described operational amplifier connects described gate controlled switch, three described gate controlled switches are with the logical course conducting of one-out-three.
2. the supply unit of a kind of electrophoresis system as claimed in claim 1, is characterized in that: described input unit is made up of the A.C. contactor of connecting successively, earth leakage protective device and fuse protector.
3. the supply unit of a kind of electrophoresis system as claimed in claim 1 or 2, it is characterized in that: described switch transducing unit comprises full-bridge phase shifting switch control module and have employed the full-bridge high-frequency switching circuit of soft switch technique, the input of described full-bridge phase shifting switch control module connects described control unit, output connects described full-bridge high-frequency switching circuit through a high_voltage isolation driver module, the input of described full-bridge high-frequency switching circuit connects the output of described pre-voltage regulation unit, and output connects the input of described high pressure conversion output unit.
4. the supply unit of a kind of electrophoresis system as claimed in claim 1 or 2, it is characterized in that: described high pressure conversion output unit is by the high pressure full-wave rectifying circuit of connecting successively, high-pressure filter circuit, high pressure EMI filter circuit and voltage, current sampling circuit, and output control circuit is formed; The output of described voltage, current sampling circuit connects described control unit respectively by a voltage sampling signal conditioning module, a current sampling signal conditioning module; The control end of described output control circuit connects described control unit by a high_voltage isolation driver module, and output connects described electrophoresis system disengagement chamber electrode.
5. the supply unit of a kind of electrophoresis system as claimed in claim 3, it is characterized in that: described high pressure conversion output unit is by the high pressure full-wave rectifying circuit of connecting successively, high-pressure filter circuit, high pressure EMI filter circuit and voltage, current sampling circuit, and output control circuit is formed; The output of described voltage, current sampling circuit connects described control unit respectively by a voltage sampling signal conditioning module, a current sampling signal conditioning module; The control end of described output control circuit connects described control unit by a high_voltage isolation driver module, and output connects described electrophoresis system disengagement chamber electrode.
6. the supply unit of a kind of electrophoresis system as described in claim 1 or 2 or 5, is characterized in that: described voltage, current sampling signal conditioning module are formed by the isolation photoelectric coupled circuit of connecting and AD Acquisition Circuit.
7. the supply unit of a kind of electrophoresis system as claimed in claim 3, is characterized in that: described voltage, current sampling signal conditioning module are formed by the isolation photoelectric coupled circuit of connecting and AD Acquisition Circuit.
8. the supply unit of a kind of electrophoresis system as claimed in claim 4, is characterized in that: described voltage, current sampling signal conditioning module are formed by the isolation photoelectric coupled circuit of connecting and AD Acquisition Circuit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310133053.6A CN103199714B (en) | 2013-04-17 | 2013-04-17 | Power source device of electrophoresis system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310133053.6A CN103199714B (en) | 2013-04-17 | 2013-04-17 | Power source device of electrophoresis system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103199714A CN103199714A (en) | 2013-07-10 |
| CN103199714B true CN103199714B (en) | 2015-03-18 |
Family
ID=48722107
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310133053.6A Active CN103199714B (en) | 2013-04-17 | 2013-04-17 | Power source device of electrophoresis system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103199714B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103812367B (en) * | 2014-03-10 | 2016-05-04 | 重庆理工大学 | A kind ofly export controlled voltage-stabilized power supply |
| CN103997234A (en) * | 2014-04-24 | 2014-08-20 | 北京市六一仪器厂 | Split electrophoresis apparatus power supply |
| CN104166419B (en) * | 2014-08-29 | 2017-01-18 | 江苏天瑞仪器股份有限公司 | Controllable high-voltage direct current power supply |
| CN105445620B (en) * | 2015-11-27 | 2018-05-01 | 云南电网有限责任公司电力科学研究院 | A kind of High voltage output device and method for feeder automation tester |
| CN107809102A (en) * | 2017-11-13 | 2018-03-16 | 江苏天瑞仪器股份有限公司 | A kind of mass spectrometer detector high tension protection circuit |
| CN108462396B (en) * | 2018-03-29 | 2024-02-23 | 青岛华电高压电气有限公司 | Controllable high-voltage direct-current power supply of 35kV oscillatory wave system |
| CN111560063B (en) * | 2020-05-12 | 2022-11-01 | 蚌埠医学院 | Raw material medicine purification device for animal pancreas-derived insulin and use method |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7324354B2 (en) * | 2005-07-08 | 2008-01-29 | Bio-Rad Laboratories, Inc. | Power supply with a digital feedback loop |
| CN101804496B (en) * | 2010-03-24 | 2012-06-13 | 河海大学常州校区 | Two-stage consecutive PWM control method of soft switch inverter arc-welding power supply |
| CN102611329A (en) * | 2011-01-24 | 2012-07-25 | 江苏清能电源有限公司 | High frequency inversion rectifying device for three-phase high-power low voltage and large current and method thereof |
-
2013
- 2013-04-17 CN CN201310133053.6A patent/CN103199714B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103199714A (en) | 2013-07-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103199714B (en) | Power source device of electrophoresis system | |
| CN101834539B (en) | High-efficiency AC/DC combined converter with wide output voltage range | |
| CN109861546B (en) | Power electronic transformer with true bipolar direct current output capability and application control | |
| CN203243237U (en) | Power supply device of electrophoresis system | |
| CN105517650B (en) | Multiple coil inverse-excitation type DC/AC translation circuit | |
| CN205430082U (en) | Contravariant welding machine electrical power generating system with power factor correction function | |
| CN107546959A (en) | A kind of Switching Power Supply, electronic equipment and Switching Power Supply control method | |
| CN105743356A (en) | LLC resonance converter | |
| CN108281979A (en) | A kind of electronics feedback load circuit of suitable low-voltage, high-current input | |
| CN110048520A (en) | Primary side has the wireless charging device of C-LCC compensation network | |
| CN108418422B (en) | Power supply system compatible with single-phase and three-phase input | |
| CN104578820B (en) | A kind of high power density AC great current generator | |
| CN105305853A (en) | Multi-pulse wave rectifier using active power factor correction technology and design method thereof | |
| CN206100548U (en) | Electrical power generating system based on ARM control system realizes drive of single -stage PFCLED | |
| CN104578750B (en) | A kind of offset-type power supply | |
| CN109245568A (en) | A kind of exchange turns direct-current isolating switch power circuit | |
| CN206506452U (en) | A kind of switching power source control circuit of wide scope input | |
| CN203562977U (en) | Photovoltaic inverter of Buck-Boost type topological structure | |
| CN108599163A (en) | The working method of intermediate frequency furnace harmonic filter system | |
| CN202663316U (en) | Circuit with three common-grounded power supplies of high voltage alternating current, low voltage alternating current and low voltage direct current | |
| CN201766511U (en) | Iron core-free alternating current voltage stabilizer | |
| CN106130384B (en) | Induction electric energy based on auxiliary induction transmits circulation control circuit system | |
| CN208548830U (en) | A kind of power factor regulation unit of ultrasonic power | |
| CN204810140U (en) | Power factor correction circuit and invertion power supply circuit, contravariant welding machine | |
| CN105245107B (en) | A kind of current mode AC power converter |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |