CN103199714A - Power source device of electrophoresis system - Google Patents
Power source device of electrophoresis system Download PDFInfo
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- CN103199714A CN103199714A CN2013101330536A CN201310133053A CN103199714A CN 103199714 A CN103199714 A CN 103199714A CN 2013101330536 A CN2013101330536 A CN 2013101330536A CN 201310133053 A CN201310133053 A CN 201310133053A CN 103199714 A CN103199714 A CN 103199714A
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Abstract
The invention relates to a power source device of an electrophoresis system. The power source device of the electrophoresis system comprises an input unit, a pre-voltage-stabilization unit, a switch transduction unit and a high-voltage transformation output unit, wherein the input unit, the pre-voltage-stabilization unit, the switch transduction unit and the high-voltage transformation output unit are connected in sequence. Grid power frequency alternating voltage is converted into a programmable error-corrected direct current power source firstly, then the direct current power source is converted into high voltage pulse signals, and then the high voltage pulse signals are converted into high voltage direct voltage and a high voltage direct current, wherein the high voltage direct voltage and the high voltage direct current are needed by the electrophoresis system. The power source device of the electrophoresis system is characterized in that the pre-voltage-stabilization unit, the switch transduction unit and the high-voltage transformation output unit and a control unit construct closed loop feedback control. The closed loop feedback control can conduct self-adaption regulations according to power source requirements of the electrophoresis system, load changes of a buffer solution and output error analysis results of the high-voltage transformation output unit, and supply the stable high voltage direct voltage and the stable high voltage direct current for the electrophoresis system in a working method of constant voltage or constant current or constant power. The power source device of the electrophoresis system can be used for an electrophoresis system which is high in requirement for power source stability and can enable a potential of hydrogen (pH) gradient of the buffer solution in the electrophoresis system to be more stable.
Description
Technical field
The present invention relates to a kind of supply unit, particularly about a kind of supply unit that can be used in large-scale liquid phase isoelectric focusing electrophoresis system.
Background technology
In recent years, liquid phase isoelectric focusing electrophoresis technology has injected new vitality with separating for the analysis of large biological molecule.As protein analysis with separate aspect, liquid phase isoelectric focusing electrophoresis technology can be reduced to minimum with the loss of protein, can be competent at comparatively meticulous protein analysis work, and the preparation separating experiment of finishing protein.In liquid phase isoelectric focusing electrophoresis system, the stability of pH of buffer gradient is a key factor that influences the Separation of Proteins quality always.In order to guarantee the stability of pH of buffer gradient, people set about from the mode of filling buffer solution: wherein, a kind of mode is that the mixture solution that directly will have the different ampholytes of low electrophoretic mobility injects disengagement chamber, under effect of electric field, progressively form a natural pH gradient; Another kind of mode is that at different buffer solution inlets, the parallel multiple buffer solution that injects different pH forms an artificial pH gradient simultaneously.Above-mentioned dual mode, the uninterrupted sample of going up of the continuous filling of buffer solution and sample, the electricity that all can cause separating the electric field arrival end is led instability, thereby influences the separation accuracy of electrophoresis.
When Separation of Proteins, the pH gradient of buffer solution is maintained by electric field in the liquid phase isoelectric focusing electrophoresis system, and stable p H gradient depends on the adaptive load ability of system power supply.Existing liquid phase isoelectric focusing electrophoresis system is general, and what use is traditional " Constant power supply ", just the user in use, " Constant power supply " once can only export a kind of in constant voltage, constant current or the permanent power.If the value of setting of voltage or electric current can be adjusted and then keep to " Constant power supply " adaptively, so just can prolong the stabilization time of disengagement chamber front end pH gradient, therefore, development possesses the controllable high-voltage stabilized power supply of adaptive ability, to guarantee the stability of pH of buffer gradient in the free stream cataphoresis system better, it is the general trend of current research.
Summary of the invention
At the problems referred to above, the purpose of this invention is to provide a kind of can be as required, with the working method of constant voltage, constant current or permanent power, provide the supply unit of the electrophoresis system of stable high-voltage DC power supply adaptively.
For achieving the above object, the present invention takes following technical scheme: a kind of supply unit of electrophoresis system is characterized in that: it comprises input unit, pre-voltage regulation unit, switch transducing unit, high pressure conversion output unit, control unit and accessory power supply unit; The input unit input connects electrical network, and output connects pre-voltage regulation unit and accessory power supply unit input respectively, cuts off the input of electric network source when abnormal conditions take place; Pre-voltage regulation unit output connects switch transducing unit input, and the power frequency ac voltage that input unit is provided is converted to direct current output, exports to switch transducing unit, also direct current output and electrical network is isolated simultaneously; Switch transducing unit output connects high pressure conversion output unit input, and the direct current output that pre-voltage regulation unit is provided is converted to high-voltage pulse signal, exports to the high pressure conversion output unit; High pressure conversion output unit output connects electrophoresis system disengagement chamber electrode, and the high-voltage pulse signal that switch transducing unit is provided is converted to high-voltage dc voltage, the electric current that electrophoresis system can use; Control unit connects host computer by data/address bus, carry out data interaction with host computer, connect high pressure conversion output unit output by data/address bus, gather the high-voltage dc voltage of high pressure conversion output unit output, electric current, connect pre-voltage regulation unit control end by data/address bus, according to the power requirement of electrophoresis system and the variation of buffer solution load, and high pressure conversion output unit output error analysis result, regulate and control the direct current output of pre-voltage regulation unit, connect switch transducing unit controls end, reverse frequency and the duty ratio of the high-voltage pulse signal of regulating switch transducing unit output by data/address bus; Connect high pressure conversion output unit control end, the output of opening or turn-offing the high pressure conversion output unit according to the control command of host computer by data/address bus; Accessory power supply unit output connects control unit, for it provides operating voltage.
Above-mentioned pre-voltage regulation unit is made of high-frequency isolation transformer, surge protecting circuit, electromagnetic compatibility and Electromagnetic interference filter, full-wave rectifier filter circuit, electric current and voltage temperature current foldback circuit and the three permanent feedback control circuits of series connection successively; Three permanent feedback control circuits comprise voltage, electric current, three FEEDBACK CONTROL branches of power, include a direct current energy supply control module in each FEEDBACK CONTROL branch, one output feedback control module, one operational amplifier and a gate controlled switch, the input of DC power supply control module connects control unit, output is by high pressure isolation drive module and D/A converter module concatenation operation amplifier input terminal, the input of output feedback control module connects control unit, output is by high pressure isolation drive module and D/A converter module concatenation operation amplifier input terminal, and the output of operational amplifier connects gate controlled switch; Three gate controlled switches select one logical course conducting with three.
Above-mentioned input unit is made of A.C. contactor, earth leakage protective device and the fuse protection device of series connection successively.
Above-mentioned switch transducing unit comprises the full-bridge phase shifting switch control module and has adopted the full-bridge high-frequency switching circuit of soft switch technique, the input of full-bridge phase shifting switch control module connects control unit, output connects the full-bridge high-frequency switching circuit through a high pressure isolation drive module, the input of full-bridge high-frequency switching circuit connects the output of pre-voltage regulation unit, and output connects the input of high pressure conversion output unit.
Above-mentioned high pressure conversion output unit is by high pressure full-wave rectifying circuit, high-pressure filter circuit, high pressure EMI filter circuit and voltage, the current sampling circuit of series connection successively, and output control circuit constitutes; The output of voltage, current sampling circuit connects control unit by a voltage sampling signal conditioning module, a current sampling signal conditioning module respectively; The control end of output control circuit connects control unit by a high pressure isolation drive module, and output connects electrophoresis system disengagement chamber electrode.
Above-mentioned voltage, current sampling signal conditioning module constitute by isolation light lotus root circuit and the AD Acquisition Circuit of series connection.
The present invention is owing to take above technical scheme, it has the following advantages: 1, the present invention is by arranging a control unit, according to the power requirement of electrophoresis system and the variation of buffer solution load, adjust voltage, current value, the power rating of pre-voltage regulation unit output in the supply unit adaptively, can when the separation of liquid phase isoelectric focusing electrophoresis system is combined with the protein sample of dyestuff, the deviation angle of its initial swimming obviously be strengthened.2, the present invention is by arranging a control unit, real-time accurate is gathered the actual output of power supply, pass through related operation, be converted into error controling signal, three permanent outputs of FEEDBACK CONTROL supply voltage, electric current, power, thereby can effectively reduce the power supply output pulsation, further improve the stability of power supply output, make in the electrophoresis system pH of buffer gradient more stable.3, the present invention is mutual by a control unit and host computer are set, the range of liquid phase isoelectric focusing electrophoresis system power supply is set by host computer, and return the operating state of power supply in real time, the operation of realization program-controlled intelligent, in addition, can also in time open as required or the output of break-make power supply, strengthen the security performance of supply unit.The present invention can be used for the electrophoresis system higher to the power supply stability requirement, as liquid phase isoelectric focusing electrophoresis system, thereby makes in the electrophoresis system pH of buffer gradient more stable.
Description of drawings
Fig. 1 is composition schematic diagram of the present invention;
Fig. 2 is the composition schematic diagram of input unit of the present invention;
Fig. 3 is the composition schematic diagram of pre-voltage regulation unit of the present invention;
Fig. 4 is the composition schematic diagram of three permanent feedback control circuits of pre-voltage regulation unit of the present invention;
Fig. 5 is the connection diagram of the full-bridge phase shifting switch control module of switch transducing of the present invention unit;
Fig. 6 is the composition schematic diagram of the full-bridge high-frequency switching circuit of switch transducing of the present invention unit;
Fig. 7 is the composition schematic diagram of high pressure conversion output unit of the present invention;
Fig. 8 is the voltage of high pressure conversion output unit of the present invention, the composition schematic diagram of current sampling circuit;
Fig. 9 is the connection diagram of the high pressure isolation drive module of high pressure conversion output unit of the present invention;
Figure 10 is the power supply output relation schematic diagram of the present invention under constant voltage, constant current, permanent power operating state.
Embodiment
Below in conjunction with drawings and Examples the present invention is described in detail.
As shown in Figure 1, the present invention mainly is made up of input unit 10, pre-voltage regulation unit 20, switch transducing unit 30, high pressure conversion output unit 40, control unit 50 and accessory power supply unit 60.Wherein:
Switch transducing unit 30 outputs connect high pressure conversion output unit 40 inputs, and the direct current output that is used for pre-voltage regulation unit 20 is provided is converted to high-voltage pulse signal, exports to high pressure conversion output unit 40.
High pressure conversion output unit 40 outputs connect electrophoresis system disengagement chamber electrode, and the high-voltage pulse signal that is used for switch transducing unit 30 is provided is converted to high-voltage dc voltage, the electric current that electrophoresis system can use.
1) connects host computer 70 by data/address bus, carry out data interaction with host computer 70, receive the control command of host computer 70, the working condition of feedback supply unit of the present invention;
2) connect high pressure conversion output unit 40 outputs by data/address bus, gather high-voltage dc voltage, the electric current of 40 outputs of high pressure conversion output unit, to carry out error analysis;
3) connect pre-voltage regulation unit 20 control ends by data/address bus, with according to the power requirement of electrophoresis system and the variation of buffer solution load, and high pressure conversion output unit 40 output error analysis results, regulate and control the direct current output of pre-voltage regulation unit 20, realize with constant voltage, constant current or permanent power being the closed loop feedback control of purpose;
4) connect switch transducing unit 30 control ends by data/address bus, reverse frequency and the duty ratio of the high-voltage pulse signal of regulating switch transducing unit 30 outputs are to realize voltage stabilizing control;
5) connect high pressure conversion output unit 40 control ends, the output of opening or turn-offing high pressure conversion output unit 40 according to the control command of host computer 70 by data/address bus.
Accessory power supply unit 60 outputs connect control unit 50, for control unit 50 provides operating voltage.
Describe the concrete setting of above-mentioned each unit in detail below in conjunction with accompanying drawing:
As shown in Figure 2, input unit 10 of the present invention can be made of A.C. contactor 17, earth leakage protective device 18 and the fuse protection device 19 of series connection successively.Wherein, the input of A.C. contactor 17 is connected with the electrical network output, and the output of fuse protection device 19 is connected with the input of pre-voltage regulation unit 20 and accessory power supply unit 60 respectively.
As shown in Figure 3, pre-voltage regulation unit 20 of the present invention can be made of high-frequency isolation transformer 21, surge protecting circuit 22, electromagnetic compatibility and Electromagnetic interference filter 23, full-wave rectifier filter circuit 24, electric current and voltage temperature the current foldback circuit 25 and three permanent feedback control circuits 26 of series connection successively.Wherein, the input of high-frequency isolation transformer 21 is connected with the output of fuse protection device 19 in the input unit 10.The output of three permanent feedback control circuits 26 is connected with the input of switch transducing unit 30.Full-wave rectifier filter circuit 24 is converted to pre-steady direct voltage with the alternating voltage of power frequency.
As shown in Figure 4, three permanent feedback control circuits 26 are mainly by voltage, electric current, three FEEDBACK CONTROL branches of power constitute, include a direct current energy supply control module in each FEEDBACK CONTROL branch, one output feedback control module, one operational amplifier and a gate controlled switch, the input of DC power supply control module connects control unit 50, output is by a high pressure isolation drive module and a D/A converter module (D/A) concatenation operation amplifier input terminal, the input of output feedback control module connects control unit 50, output is by a high pressure isolation drive module and a D/A converter module (D/A) concatenation operation amplifier input terminal, and the output of operational amplifier connects gate controlled switch.Above-mentioned three gate controlled switches select one logical course conducting with three, thereby make three permanent feedback control circuits 26 give the switch transducing unit 30 of back one-level with working method output dc voltage, the electric current of constant voltage, constant current or permanent power.Wherein, the constant voltage working method: the DC power supply control module in the voltage FEEDBACK CONTROL branch arranges under the instruction at the voltage that control unit sends; through high pressure isolation drive module and D/A converter module; the output voltage signalization; export feedback control module simultaneously under the error correction instruction that control unit sends; the output voltage error signal; voltage signalization and the voltage error signal pre-steady direct voltage that acting in conjunction is exported in electric current and voltage temperature current foldback circuit 25 after the operational amplifier stack; it is adjusted into the stable direct voltage that meets target, offers the switch transducing unit 30 of back one-level.The working method of constant current or permanent power and the working method of constant voltage are similar, repeat no more herein.
As shown in Figure 5, switch transducing of the present invention unit 30 can be made of the full-bridge high-frequency switching circuit 31 that has adopted soft switch technique and full-bridge phase shifting switch control module 32.The input of full-bridge phase shifting switch control module 32 connects control unit 50, output connects full-bridge high-frequency switching circuit 31 through high pressure isolation drive module, the input of full-bridge high-frequency switching circuit 31 connects the output of pre-voltage regulation unit 20, and output connects the input of high pressure conversion output unit 40.So-called soft switch technique refers to increase resonant elements such as inductance, electric capacity in circuit, introduce resonance before and after switching process, to eliminate the overlapping of voltage, electric current, reduces switching loss and switching noise.
As shown in Figure 6, a kind of full-bridge high-frequency switching circuit 31 that has adopted soft switch technique: take turns conducting as the MOSFET power tube A/C of switching tube and B/D, differ 180 ° of phase angles, but A and D or B and C conducting simultaneously, A and the conducting of B elder generation are called leading-bridge; Conducting behind C and the D is called lagging leg.Wherein, leading-bridge is realized zero voltage switch ZVS(Zero Voltage Switch easily), main output inductor participates in resonance, the impulse electricity needs of shunt capacitance C1 and C2 in the time of can satisfying switching tube A and B shutoff; Lagging leg is because in the turn off process, the secondary short circuit of high tension transformer 33, have only the former limit leakage inductance of high tension transformer 33 to participate in resonance, can not finish the impulse electricity work of shunt capacitance C3 and C4 rapidly, therefore need increase former limit resonant inductance Lr on the former limit of lagging leg one side, to realize zero voltage switch.Because the increase of former limit resonant inductance Lr can prolong primary current at the transformation period of positive and negative half period, cause the Loss Rate of secondary duty ratio to raise easily, reduce power-efficient, so former limit resonant inductance Lr value is unsuitable excessive.Zero voltage switch essence is to utilize resonant process to the impulse electricity of shunt capacitance, allows a certain brachium pontis mid-point voltage be raised to the magnitude of voltage of input (also being the dc voltage value of pre-voltage regulation unit 20 outputs) fast or drops to null value.When the switching tube A of same brachium pontis and B or C and D were about to connect, its diode connected in parallel D1 and D2 or D3 and D4 connected, with the both end voltage pincers of corresponding switching tube at 0V, for realizing that zero voltage switch creates conditions.In order to make lagging leg realize zero voltage switch better, can be at the two ends of lagging leg an auxiliary resonant net (inductance L a among Fig. 6, capacitor C 5, C6 in parallel, the circuit that diode D5, D6 constitute), neither disturb major loop, can reduce secondary duty-cycle loss rate again, improve power-efficient.
Switching tube A/B in the full-bridge high-frequency switching circuit 31 and C/D are driven by full-bridge phase shifting switch control module 32.Full-bridge phase shifting switch control module 32 output signals, reach the two couples of switching tube A/B and C/D in the full-bridge high-frequency switching circuit 31 through high pressure isolation drive module, make it with PWM mode alternate conduction and end, thereby the direct voltage that pre-voltage regulation unit 20 is provided is reverse into the high frequency square wave alternating voltage, is sent to the former limit of high tension transformer 33.High tension transformer 33 can reach several kilovolts high-voltage pulse signal by transformation of electrical energy in its secondary generation.Wherein, switching frequency and PWM duty ratio (also being reverse frequency and the duty ratio of high-voltage pulse signal) are set by control unit 50, can reach the voltage stabilizing control effect of output voltage like this.Take place simultaneously when unusual, full-bridge phase shifting switch control module 32 can also cut off the output of full-bridge high-frequency switching circuit 31, plays the effect of protective circuit device.
As shown in Figure 7, high pressure conversion output circuit 40 of the present invention can be by high pressure full-wave rectifying circuit 41, high-pressure filter circuit 42, high pressure EMI filter circuit 43 and voltage, the current sampling circuit 44 of series connection successively, and output control circuit 45 constitutes.Wherein, the input of high pressure full-wave rectifying circuit 41 is connected with the output of switch transducing unit 30.The output of output control circuit 45 is connected with electrophoresis system disengagement chamber electrode.High-voltage pulse signal rectification, filtering that high pressure full-wave rectifying circuit 41 and high-pressure filter circuit 42 provide switch transducing unit 30, and then convert high-voltage dc voltage to; Wherein, high-pressure filter circuit 42 is made up of output inductor device and electric capacity, and the parameter of output inductor device is set maximum and the multiple factors such as maximum fluctuation electric current, inductance core, power capacity and filter capacitor that need to consider filter circuit output effective current.In general, the current pulsation value that can choose output inductor is 20% of maximum output current, and when 1/2nd pulsating quantities, output inductor should keep continuous state simultaneously.High pressure EMI filter circuit 43 is used for further improving the antijamming capability of high-voltage dc voltage, reduces electromagnetic noise, removes ripple and disturbs.
As shown in Figure 8, high-voltage dc voltage, the electric current of voltage, 43 outputs of 44 couples of hv filtering EMI of current sampling circuit circuit carry out resistance capacitance balance voltage stabilization and current stabilization filtering sampling, and the voltage of gathering, current signal are sent into control unit 50 through a voltage sampling signal conditioning module, a current sampling signal conditioning module respectively.Described voltage, current sampling signal conditioning module all can be by isolation light lotus root circuit and the AD(moduluses of series connection) Acquisition Circuit constitutes.In addition, because high-pressure leakage takes place the high tension transformer 33 in the switch transducing unit 30 easily, so the preferred low temperature of the present invention floats precision resistance structure voltage, current sampling circuit 44.
As shown in Figure 9, the control end of output control circuit 45 connects control unit 50 by a high pressure isolation drive module, opens or turn-off high voltage direct current output under the control of control unit 50, also is power supply output of the present invention, to guarantee the electrophoresis system job security.
In above-described embodiment, can adopt digital isolating chip ADUM1410 as ADI company as the high pressure isolation drive module among Fig. 4, Fig. 5 and Fig. 9, control unit 50 and the high-tension circuits such as pre-voltage regulation unit 20, switch transducing unit 30 and high transformation output unit 40 of its control are isolated.
In above-described embodiment, can adopt singlechip control chip to make control unit 50.
In above-described embodiment, can adopt the UC3879 main control chip to make full-bridge phase shifting switch control module 32.
In above-described embodiment, in the supply unit overvoltage, overcurrent, thermal-shutdown circuit can also be set, and corresponding fault indication signal is fed back to control unit 50.
As shown in figure 10, be the output of the present invention under constant voltage, constant current, permanent power operating state, its voltage V, electric current I, power P and the time dependent schematic diagram of load R:
When isoelectric focusing electrophoresis, the pH gradient of Separation of Proteins buffer solution is to maintain by electric field, and stable p H gradient depends on the adaptive load ability of liquid phase isoelectric focusing electrophoresis system stability power supply.In the electrophoresis process, can be the equivalence of electrophoresis cavity a load resistance, its resistance height be determined by the ionic strength of buffer system.At the electrophoresis initial time, because the impedance of buffer solution is less, electric current is bigger, and when surpassing a certain limit, the Joule heat of generation just may cause the system works failure.Therefore need set in advance upper current limit value I
W, when electric current reaches I
WThe time, the present invention starts control unit, works with the constant current output state.T0~t1 is exactly the constant current output stage.At the constant current output stage, the trend swimming of ampholytes causes the voltage between iontophoretic electrode to raise and the power output increase in the buffer system.According to the exchange capacity of system to Joule heat, under the prerequisite of the system of assurance operate as normal, set in advance the upper limit P of power output
WWhen power output reaches P
WThe time, the present invention starts control unit, with permanent power output state work.T1~t2 is exactly permanent power output stage.At permanent power output stage, voltage is still rising, and electric current is descending, and the product of voltage and current remains unchanged.Along with continuing to increase of electrophoresis system impedance RL, output voltage continues to rise.Therefore need set in advance the higher limit U of voltage
W, when voltage reaches U
WThe time, the present invention starts control unit, works with the constant voltage output state.Be exactly the constant voltage output stage after the t2.
The various embodiments described above only are used for explanation the present invention, and wherein the structure of each parts, connected mode etc. all can change to some extent, and every equivalents and improvement of carrying out on the basis of technical solution of the present invention all should do not got rid of outside protection scope of the present invention.
Claims (10)
1. the supply unit of an electrophoresis system is characterized in that: it comprises input unit, pre-voltage regulation unit, switch transducing unit, high pressure conversion output unit, control unit and accessory power supply unit;
Described input unit input connects electrical network, and output connects described pre-voltage regulation unit and described accessory power supply unit input respectively, cuts off the input of electric network source when abnormal conditions take place;
Described pre-voltage regulation unit output connects described switch transducing unit input, and the power frequency ac voltage that described input unit is provided is converted to direct current output, exports to described switch transducing unit, also direct current output and electrical network is isolated simultaneously;
Described switch transducing unit output connects described high pressure conversion output unit input, and the direct current output that described pre-voltage regulation unit is provided is converted to high-voltage pulse signal, exports to described high pressure conversion output unit;
Described high pressure conversion output unit output connects electrophoresis system disengagement chamber electrode, and the high-voltage pulse signal that described switch transducing unit is provided is converted to high-voltage dc voltage, the electric current that described electrophoresis system can use;
Described control unit connects host computer by data/address bus, carry out data interaction with described host computer, connect described high pressure conversion output unit output by data/address bus, gather the high-voltage dc voltage of described high pressure conversion output unit output, electric current, connect described pre-voltage regulation unit control end by data/address bus, according to the power requirement of described electrophoresis system and the variation of buffer solution load, and described high pressure conversion output unit output error analysis result, regulate and control the direct current output of described pre-voltage regulation unit, connect described switch transducing unit controls end by data/address bus, regulate and control reverse frequency and the duty ratio of the high-voltage pulse signal of described switch transducing unit output; Connect described high pressure conversion output unit control end by data/address bus, according to the control command unlatching of described host computer or the output of turn-offing described high pressure conversion output unit;
Described accessory power supply unit output connects described control unit, for it provides operating voltage.
2. the supply unit of a kind of electrophoresis system as claimed in claim 1 is characterized in that: described pre-voltage regulation unit is made of high-frequency isolation transformer, surge protecting circuit, electromagnetic compatibility and Electromagnetic interference filter, full-wave rectifier filter circuit, electric current and voltage temperature current foldback circuit and the three permanent feedback control circuits of series connection successively; Described three permanent feedback control circuits comprise voltage, electric current, three FEEDBACK CONTROL branches of power, include a direct current energy supply control module in each described FEEDBACK CONTROL branch, one output feedback control module, one operational amplifier and a gate controlled switch, the input of described DC power supply control module connects described control unit, output is connected the input of described operational amplifier with D/A converter module by high pressure isolation drive module, the input of described output feedback control module connects described control unit, output is connected the input of described operational amplifier by high pressure isolation drive module with D/A converter module, the output of described operational amplifier connects described gate controlled switch; Three described gate controlled switches select one logical course conducting with three.
3. the supply unit of a kind of electrophoresis system as claimed in claim 1 is characterized in that: described input unit is made of A.C. contactor, earth leakage protective device and the fuse protection device of series connection successively.
4. the supply unit of a kind of electrophoresis system as claimed in claim 2 is characterized in that: described input unit is made of A.C. contactor, earth leakage protective device and the fuse protection device of series connection successively.
5. as the supply unit of claim 1 or 2 or 3 or 4 described a kind of electrophoresis systems, it is characterized in that: described switch transducing unit comprises the full-bridge phase shifting switch control module and has adopted the full-bridge high-frequency switching circuit of soft switch technique, the input of described full-bridge phase shifting switch control module connects described control unit, output connects described full-bridge high-frequency switching circuit through a high pressure isolation drive module, the input of described full-bridge high-frequency switching circuit connects the output of described pre-voltage regulation unit, and output connects the input of described high pressure conversion output unit.
6. as the supply unit of claim 1 or 2 or 3 or 4 described a kind of electrophoresis systems, it is characterized in that: described high pressure conversion output unit is by high pressure full-wave rectifying circuit, high-pressure filter circuit, high pressure EMI filter circuit and voltage, the current sampling circuit of series connection successively, and output control circuit constitutes; The output of described voltage, current sampling circuit connects described control unit by a voltage sampling signal conditioning module, a current sampling signal conditioning module respectively; The control end of described output control circuit connects described control unit by a high pressure isolation drive module, and output connects described electrophoresis system disengagement chamber electrode.
7. the supply unit of a kind of electrophoresis system as claimed in claim 5, it is characterized in that: described high pressure conversion output unit is by high pressure full-wave rectifying circuit, high-pressure filter circuit, high pressure EMI filter circuit and voltage, the current sampling circuit of series connection successively, and output control circuit constitutes; The output of described voltage, current sampling circuit connects described control unit by a voltage sampling signal conditioning module, a current sampling signal conditioning module respectively; The control end of described output control circuit connects described control unit by a high pressure isolation drive module, and output connects described electrophoresis system disengagement chamber electrode.
8. as the supply unit of claim 1 or 2 or 3 or 4 or 7 described a kind of electrophoresis systems, it is characterized in that: described voltage, current sampling signal conditioning module constitute by isolation light lotus root circuit and the AD Acquisition Circuit of series connection.
9. the supply unit of a kind of electrophoresis system as claimed in claim 5 is characterized in that: described voltage, current sampling signal conditioning module constitute by isolation light lotus root circuit and the AD Acquisition Circuit of series connection.
10. the supply unit of a kind of electrophoresis system as claimed in claim 6 is characterized in that: described voltage, current sampling signal conditioning module constitute by isolation light lotus root circuit and the AD Acquisition Circuit of series connection.
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CN104166419A (en) * | 2014-08-29 | 2014-11-26 | 江苏天瑞仪器股份有限公司 | Controllable high-voltage direct current power supply |
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