CN103197056B - Enzyme-labeling instrument - Google Patents
Enzyme-labeling instrument Download PDFInfo
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- CN103197056B CN103197056B CN201310099170.5A CN201310099170A CN103197056B CN 103197056 B CN103197056 B CN 103197056B CN 201310099170 A CN201310099170 A CN 201310099170A CN 103197056 B CN103197056 B CN 103197056B
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- 238000002372 labelling Methods 0.000 title abstract 5
- 230000003287 optical effect Effects 0.000 claims abstract description 71
- 230000007246 mechanism Effects 0.000 claims abstract description 37
- 238000006243 chemical reaction Methods 0.000 claims abstract description 36
- 230000005693 optoelectronics Effects 0.000 claims description 37
- 238000002965 ELISA Methods 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 3
- 230000005622 photoelectricity Effects 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 abstract description 7
- 108090000790 Enzymes Proteins 0.000 abstract description 7
- 239000013307 optical fiber Substances 0.000 abstract description 5
- 230000005540 biological transmission Effects 0.000 abstract 1
- 238000004737 colorimetric analysis Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004134 energy conservation Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010205 computational analysis Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
Abstract
The invention discloses an enzyme-labeling instrument. Optical centers of a light source, an upper lens, a middle lens and a lower lens of each group of photoelectric conversion mechanism of the enzyme-labeling instrument are arranged on a straight line, the straight line is set to be an optical centerline, the optical center line passes through the light receiving surfaces of photoelectric cells of the corresponding photoelectric conversion mechanisms, each group of photoelectric conversion mechanism is provided with a plurality of optical filters with different wavelengths, the plurality of optical filters are fixed on an optical filter rack, the optical filter rack is connected with a driving device for driving the optical filter rack to horizontally move, and a workbench used for bearing an enzyme label plate is arranged between the upper lens and the middle lens of the photoelectric conversion mechanism. The enzyme-labeling instrument does not need optical fiber in the whole optical path transmission process, so that the enzyme-labeling instrument is simple in overall structure, small in occupying space and low in light source loss, can meet the requirements on enzyme standard colorimetry by using a light source with low energy, and saves energy.
Description
Technical field
The present invention relates to a kind of clinical treatment equipment, particularly a kind of microplate reader.
Background technology
Microplate reader is a kind of equipment for enzyme inspection-free survey 96 hole ELISA Plate colorimetric, is a requisite equipment in the inspection-free survey of enzyme.At present, microplate reader at home situation of all-level hospitals be used widely.
Traditional microplate reader is made up of light source, optical filter runner, optical fiber and check-out console etc.During work, light source is divided into 8 light beams by optical fiber after the process of optical filter, respectively by an enzyme mark hole, 8 in ELISA Plate, the solution through integrated enzyme reaction is had in enzyme mark hole, light passes through solution, by the photoelectric cell arrived after an absorption part on check-out console, light signal is converted to electric signal input computing machine by photoelectric cell or processor carries out computational analysis.In testing process, select optical filter by rotating filtering sheet runner, obtain the light of different wave length.This structured optical fiber is longer, makes troubles to installation or removal, and takes up room, and is unfavorable for the simplification of structure.Light source power consumption is large simultaneously, energy-conservation not.
Summary of the invention
The problem to be solved in the present invention is: provide a kind of structure simple microplate reader.
For solving the problem, the present invention includes fixed mount and be arranged at least one group of opto-electronic conversion mechanism on fixed mount, opto-electronic conversion mechanism comprises the light source arranged from top to bottom successively, upper lens, intermediate lens, optical filter, lower lens and photoelectric cell, the light source of Mei Zu opto-electronic conversion mechanism, upper lens, the optical centre arrangement of intermediate lens and lower lens point-blank, this straight line is set to optical centreline, optical centreline is by the photronic sensitive surface of corresponding photoelectricity throw-over gear, there is the optical filter of multiple different wave length in Mei Zu opto-electronic conversion mechanism, multiple optical filter is fixed on a filter holder, filter holder is connected with and drives filter holder to move horizontally drive unit, one is provided with for carrying the worktable of ELISA Plate between the upper lens of all opto-electronic conversion mechanisms and the intermediate lens of all opto-electronic conversion mechanisms.
For the ease of selecting optical filter, described driving mechanism comprises motor and friction pulley, friction pulley is fixed on the output shaft of motor, all filter array of filter holder are in a row, filter holder has a directed side extended in the orientation of multiple optical filter, friction pulley contacts with the directed side of filter holder, what the organism level of motor rotated is arranged on fixed mount, motor horizontally rotates central axis and motor center axis not point-blank, and on motor, the side of the directed side of proximity filter frame is connected with extension spring.
In order to make compact overall structure reliable, described light source is incandescent lamp bulb, all light sources are all arranged on same lamp socket, lamp socket is arranged on fixed mount, all upper lens are fixedly installed on the bottom of lamp socket, all intermediate lenses are fixed on an intermediate lens seat, intermediate lens seat is fixed on fixed mount, all lower lens are fixed on a lower lens mount, filter holder is slidably arranged on lower lens mount, lower lens mount is fixed on fixed mount, all photoelectric cells are fixed on a check-out console, check-out console is fixed on fixed mount, one end of motor is hinged on lower lens mount, the other end of motor is pressed with stator, extension spring connects between motor and lower lens mount.Incandescent lamp bulb is low in energy consumption, energy-conservation, the feature of long service life, and microplate reader is before all adopt Halogen lamp LED, and power consumption is large, and efficiency is low, and the life-span is short.
For the ease of regulating the height of light source, described lamp socket leads to lamp bracket and is arranged on fixed mount, and lamp socket and lamp bracket are fixed, and lamp bracket is adjustable for height to be arranged on holder.
In order to make, in the position detecting filter holder, just to call different optical filters, described check-out console is provided with the optoelectronic switch detecting filter holder position, and filter holder is provided with catch, and catch lower end is inserted in the detection groove of optoelectronic switch.
In order to make one-piece construction compacter, described optical filter, lower lens and check-out console are arranged in reading box, and reading box is fixed on the bottom of fixed mount, and intermediate lens seat, lower lens mount are fixedly connected with fixed mount by reading box with check-out console.
One group of optical filter can be shared to make all opto-electronic conversion mechanisms, described opto-electronic conversion mechanism has many groups, Duo Zu opto-electronic conversion mechanism arranges in the vertical, multiple optical filter arranges in the horizontal, all optical filters are the strip extended in the vertical, optical filter is provided with optical filter cover plate, and cover plate is provided with multiple hole, the hole of optical filter cover plate and optical centreline one_to_one corresponding of all groups of opto-electronic conversion mechanisms in the arrangement of optical filter length direction.
For once detecting multiple sample, described opto-electronic conversion mechanism has 8 groups, and 8 groups of opto-electronic conversion mechanisms share 4 optical filters.
Beneficial effect of the present invention: the present invention is arranged due to the special position of each optical element, makes not need optical fiber in whole optic path process, makes one-piece construction simple, take up room little, light source loss is little, needs the light source of less energy can reach the requirement of enzyme mark colorimetric, saves energy.
Accompanying drawing explanation
Fig. 1 is general structure schematic diagram of the present invention;
Fig. 2 is lamp socket and upper lens scheme of installation;
Fig. 3 is optical filter and lower lens arrangement front schematic view;
Fig. 4 is optical filter and lower lens arrangement schematic rear view;
Fig. 5 is light path system schematic diagram of the present invention (dotted line mass color);
Wherein, 1, fixed mount, 2, lamp bracket, 3, circuit board, 4, lamp socket, 5, worktable, 6, fixed block, 7, reading box, 8, intermediate lens seat, 9, filter holder, 10, lower lens mount, 11, upper lens, 12, optical filter, 13, optical filter cover plate, 14, friction pulley, 15, catch, 16, lower lens, 17, motor, 18, insulated column, 19, extension spring, 20, pulling-on piece, 21, stator, 22, lower lens cover plate, 23, check-out console, 24, micropore cup, 25, intermediate lens, 26, photoelectric cell, 27, incandescent lamp bulb.
Embodiment
A kind of specific embodiment as shown in Figure 1, it comprises fixed mount 1 and is arranged at least one group of opto-electronic conversion mechanism on fixed mount 1, opto-electronic conversion mechanism comprises the light source arranged from top to bottom successively, upper lens 11, intermediate lens 25, optical filter 12, lower lens 16 and photoelectric cell 26, the light source of Mei Zu opto-electronic conversion mechanism, upper lens 11, the optical centre arrangement of intermediate lens 25 and lower lens 16 point-blank, this straight line is set to optical centreline, optical centreline is by the sensitive surface of the photoelectric cell 26 of corresponding photoelectricity throw-over gear, the optical filter 12 of Mei Zu opto-electronic conversion mechanism has multiple, multiple optical filter 12 is fixed on a filter holder 9, filter holder 9 is connected with and drives filter holder 9 to move horizontally drive unit, one is provided with for carrying the worktable 5 of ELISA Plate between the upper lens 11 of all opto-electronic conversion mechanisms and the intermediate lens of all opto-electronic conversion mechanisms.Worktable 5 can be placed one to two piece of 96 hole ELISA Plate, the hole in ELISA Plate is micropore cup 24.
Further illustrate for 8 groups of opto-electronic conversion mechanisms and 96 hole ELISA Plate below.The present invention is not limited to opto-electronic conversion mechanism 8 groups, is also not limited to only be applied in 96 hole ELISA Plate.
Light source is incandescent lamp bulb 27, and the light sent is white, i.e. complex light, and incandescent lamp bulb volume is little, is welded on circuit board 3, and 8 incandescent lamp bulbs are uniformly distributed with 9mm interval, and inserts respectively in the hole of 8 vertical directions on lamp socket 4.Power acquisition DC low-voltage, current sinking is in milliampere level, low in energy consumption, does not need high-power feed circuit.Light source heat radiation amount is little, naturally cools, does not need air blast cooling, substantially reduce system bulk.Lamp socket 4 is arranged on lamp bracket 2, adopts register pin accurately to locate.Lamp bracket 2 is arranged on fixed mount 1, and with screw fastening, on lamp bracket 2, fixed orifice adopts strip hole, has certain adjustment space in the vertical direction.Thus the height of light source can be regulated.
As shown in Figure 2, all upper lens 11 are fixedly installed on the bottom of lamp socket 4, all intermediate lenses 25 are fixed on an intermediate lens seat 8, all lower lens 16 are arranged in the bottom respective grooves of lower lens mount 10, all lower lens 16 compress with same lower lens cover plate 22, with screw, lower lens cover plate 22 is fixed on lower lens mount 10.Lower lens cover plate 22 there are a row 8 holes, corresponding with lower lens 16 position, make light can penetrate into bottom.Intermediate lens seat 8 and lower lens mount 10 are installed together, and use screw fastening.Intermediate lens 25 and lower lens about 16 one_to_one corresponding.
The bottom of holder 1 is fixed with reading box 7 by fixed block 6.Lower lens mount 10 and check-out console 23 are fixed in reading box 7.
As shown in Figure 3, this specific embodiment is provided with four optical filters 12, and each optical filter 12 is strip, and four optical filters 12 are placed on filter holder 9 in corresponding groove, and filter holder 9 is slidably arranged on lower lens mount 10.The optical filter of placement 4 kinds of different wave lengths can be selected.In a longitudinal direction, four optical filters 12 are arranged in a row at horizontal direction the length direction of all optical filters 12, each optical filter 12 covers with optical filter cover plate 13 and fixes.Optical filter cover plate 13 has a row 8 holes, pitch of holes is corresponding with intermediate lens 25 position.There is the hole corresponding with cover plate the lower end of filter holder 9, makes light continue to arrive the check-out console 23 of below after filtration after mating plate.Check-out console 23 is a kind of circuit boards being welded with 8 photoelectric cells 26, is installed on the below of lower lens mount, supports fixing, 8 photronic positions and 8 lower lens 16 position one_to_one corresponding with insulated column 18.Check-out console 23 is also provided with the optoelectronic switch for detecting filter holder 9.Filter holder 9 is provided with catch 15, and catch 15 lower end is inserted in the detection groove of optoelectronic switch.
As shown in Figure 3 and Figure 4, driving mechanism comprises motor 17 and friction pulley 14, friction pulley 14 is fixed on the output shaft of motor 17, filter holder 9 has a directed side extended in the orientation of multiple optical filter 12, friction pulley 14 contacts with the directed side of filter holder 9, one end of motor 17 is hinged on lower lens mount, the other end of motor 17 is pressed with stator 21, thus the organism level of motor 17 is rotated be arranged on fixed mount 1, motor 17 horizontally rotates central axis and motor 17 central axis not point-blank, on motor 17, the side of the directed side of proximity filter frame 9 is connected with extension spring 19, motor 17 is fixed with pulling-on piece 20, the two ends of extension spring 19 connect pulling-on piece 20 and filter holder 9 respectively.Thus friction pulley 14 is pressed in the directed side of filter holder 9.There is one deck silica gel the outer ring of friction pulley 14, and being used for increases friction force.Under the effect of extension spring 19 pulling force, friction pulley 14 presses the directed side of filter holder 9, filter holder 9 and friction pulley 14 is made to have enough friction force, can move horizontally along with the rotation of friction pulley 14, thus make different optical filters 12 aim at upper and lower lens respectively, realize the selection conversion of different wave length optical filter.
Principle of work: light path system of the present invention as shown in Figure 5, after incandescent lamp bulb 27 sends light, circular hole below lamp socket 4, vertically downward through upper lens 11, be able to optically focused, focus falls within micropore cup 24 inner hub location, and light beam starts to disperse afterwards, arrives intermediate lens 25 through bottom micropore cup 24, form parallel beam afterwards, be irradiated on optical filter 12, obtain the light of single wavelength, then arrive lower lens 16, optically focused again, absorbed by photoelectric cell 26 after convergence, and then be converted to electric signal, pass to processor analysis.
Claims (7)
1. a microplate reader, it is characterized in that: comprise fixed mount and be arranged at least one group of opto-electronic conversion mechanism on fixed mount, opto-electronic conversion mechanism comprises the light source arranged from top to bottom successively, upper lens, intermediate lens, optical filter, lower lens and photoelectric cell, the light source of Mei Zu opto-electronic conversion mechanism, upper lens, the optical centre arrangement of intermediate lens and lower lens point-blank, this straight line is set to optical centreline, optical centreline is by the photronic sensitive surface of corresponding photoelectricity throw-over gear, there is the optical filter of multiple different wave length in Mei Zu opto-electronic conversion mechanism, multiple optical filter is fixed on a filter holder, filter holder is connected with the drive unit driving filter holder to move horizontally, one is provided with for carrying the worktable of ELISA Plate between the upper lens of all opto-electronic conversion mechanisms and the intermediate lens of all opto-electronic conversion mechanisms, described drive unit comprises motor and friction pulley, friction pulley is fixed on the output shaft of motor, all filter array of filter holder are in a row, filter holder has a directed side extended in the orientation of multiple optical filter, friction pulley contacts with the directed side of filter holder, what the organism level of motor rotated is arranged on fixed mount, motor horizontally rotates central axis and motor center axis not point-blank, on motor, the side of the directed side of proximity filter frame is connected with extension spring.
2. microplate reader according to claim 1, it is characterized in that: described light source is incandescent lamp bulb, all light sources are all arranged on same lamp socket, lamp socket is arranged on fixed mount, all upper lens are fixedly installed on the bottom of lamp socket, all intermediate lenses are fixed on an intermediate lens seat, intermediate lens seat is fixed on fixed mount, all lower lens are fixed on a lower lens mount, filter holder is slidably arranged on lower lens mount, lower lens mount is fixed on fixed mount, all photoelectric cells are fixed on a check-out console, check-out console is fixed on fixed mount, one end of motor is hinged on lower lens mount, the other end of motor is pressed with stator, extension spring connects between motor and filter holder.
3. microplate reader according to claim 2, is characterized in that: described lamp socket is arranged on fixed mount by lamp bracket, and lamp socket and lamp bracket are fixed, and lamp bracket is adjustable for height to be arranged on fixed mount.
4. microplate reader according to claim 2, is characterized in that: described check-out console is provided with the optoelectronic switch detecting filter holder position, and filter holder is provided with catch, and catch lower end is inserted in the detection groove of optoelectronic switch.
5. microplate reader according to claim 2, it is characterized in that: described optical filter, lower lens and check-out console are arranged in reading box, reading box is fixed on the bottom of fixed mount, and intermediate lens seat, lower lens mount are fixedly connected with fixed mount by reading box with check-out console.
6. according to the microplate reader in claim 1-5 described in any one, it is characterized in that: described opto-electronic conversion mechanism has many groups, Duo Zu opto-electronic conversion mechanism arranges in the vertical, multiple optical filter arranges in the horizontal, all optical filters are the strip extended in the vertical, optical filter is provided with optical filter cover plate, and cover plate is provided with multiple hole, the hole of optical filter cover plate and optical centreline one_to_one corresponding of all groups of opto-electronic conversion mechanisms in the arrangement of optical filter length direction.
7. microplate reader according to claim 6, is characterized in that: described opto-electronic conversion mechanism has 8 groups, and 8 groups of opto-electronic conversion mechanisms share 4 optical filters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310099170.5A CN103197056B (en) | 2013-03-26 | 2013-03-26 | Enzyme-labeling instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310099170.5A CN103197056B (en) | 2013-03-26 | 2013-03-26 | Enzyme-labeling instrument |
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| Publication Number | Publication Date |
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| CN103197056A CN103197056A (en) | 2013-07-10 |
| CN103197056B true CN103197056B (en) | 2014-12-31 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310099170.5A Active CN103197056B (en) | 2013-03-26 | 2013-03-26 | Enzyme-labeling instrument |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109520930A (en) * | 2018-12-29 | 2019-03-26 | 苏州汶颢微流控技术股份有限公司 | A kind of elisa assay instrument |
| CN112717281B (en) * | 2021-01-14 | 2022-07-08 | 重庆翰恒医疗科技有限公司 | Medical robot platform and control method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203148945U (en) * | 2013-03-26 | 2013-08-21 | 山东博科生物产业有限公司 | Microplate reader |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1595115A (en) * | 2003-09-10 | 2005-03-16 | 开物科技股份有限公司 | Imaging type biological chip instrument |
| CN2718570Y (en) * | 2004-04-01 | 2005-08-17 | 徐新 | Optical structure for enzyme labeling instrument |
| CN201014978Y (en) * | 2007-02-12 | 2008-01-30 | 深圳雷杜生命科学股份有限公司 | Optical path device for semi-automatic biochemical analyzer |
| CN201166637Y (en) * | 2008-01-18 | 2008-12-17 | 南京神州英诺华医疗科技有限公司 | Optical physical design for novel discrete type full-automatic biochemical analysizer |
| EP2681534B1 (en) * | 2011-05-04 | 2021-08-11 | Siemens Healthcare Diagnostics Inc. | Methods, systems, and apparatus for sample illumination |
| CN202502044U (en) * | 2012-03-12 | 2012-10-24 | 深圳湃尔生物科技有限公司 | Light path device for analytical instrument |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203148945U (en) * | 2013-03-26 | 2013-08-21 | 山东博科生物产业有限公司 | Microplate reader |
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Denomination of invention: An enzyme labeling instrument Effective date of registration: 20211101 Granted publication date: 20141231 Pledgee: Overpass Branch of Jinan Rural Commercial Bank Co.,Ltd. Pledgor: BIOBASE BIODUSTRY (SHANDONG) Co.,Ltd. Registration number: Y2021370000122 |
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Date of cancellation: 20220624 Granted publication date: 20141231 Pledgee: Overpass Branch of Jinan Rural Commercial Bank Co.,Ltd. Pledgor: BIOBASE BIODUSTRY (SHANDONG) Co.,Ltd. Registration number: Y2021370000122 |
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Denomination of invention: An enzyme marker Effective date of registration: 20230206 Granted publication date: 20141231 Pledgee: Overpass Branch of Jinan Rural Commercial Bank Co.,Ltd. Pledgor: BIOBASE BIODUSTRY (SHANDONG) Co.,Ltd. Registration number: Y2023980032056 |
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Date of cancellation: 20230531 Granted publication date: 20141231 Pledgee: Overpass Branch of Jinan Rural Commercial Bank Co.,Ltd. Pledgor: BIOBASE BIODUSTRY (SHANDONG) Co.,Ltd. Registration number: Y2023980032056 |
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