CN103196878A - Portable fluorescence detector - Google Patents
Portable fluorescence detector Download PDFInfo
- Publication number
- CN103196878A CN103196878A CN2013100869968A CN201310086996A CN103196878A CN 103196878 A CN103196878 A CN 103196878A CN 2013100869968 A CN2013100869968 A CN 2013100869968A CN 201310086996 A CN201310086996 A CN 201310086996A CN 103196878 A CN103196878 A CN 103196878A
- Authority
- CN
- China
- Prior art keywords
- module
- excitation
- light
- lens
- control analysis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention relates to a test system in the field of bioengineering technology, and in particular relates to a portable fluorescence detector. The portable fluorescence detector comprises an excitation test module which consists of a light emitting diode with emission wavelengths of 400-600nm, a lens, a crack, a filter mirror and a photodiode, a preamplifier module and a control analysis module, wherein the output end of the excitation test module is connected with an input end of the preamplifier module, and the output end of the preamplifier module is connected with the input end of the control analysis module. According to the structure, the excitation test module excites a fluorescence probe to generate an emitted light, emitted light is converted into an electric signal and is amplified by the preamplifier module, the excitation effect is stable, the generation of interference signals can be reduced due to a pre-amplification electric circuit, and a noise-reducing signal can be amplified, so that the sensitivity and the precision of quantitative determination can be greatly improved. Compared with an excitation part in the prior art, the test system is obviously simple in structure, and portable, and expands the application range.
Description
Technical field
The present invention relates to a kind of detection system of technical field of bioengineering, especially a kind of portable fluorescence detector.
Background technology
The fluorescent chromatographic technology is utilized the biology techniques principle, utilizes the capillary syphonic effect of liquid flow on film, realizes quick separation and the detection of determinand in the sample.According to the biologically type difference that adopts, the fluorescent chromatographic technology can be divided into immunochromatography and nucleic acid hybridization chromatography two big class technology.
Because chromatography has simple operation, detects characteristics such as quick, therefore is applicable to many, the poor complex sample analyses of impurity, is particularly useful for the compartment analysis of biological sample.In recent years, chromatography has become biological chemistry and molecular biology analysis means commonly used; Be widely used in the detection of multiple different field as a kind of chromatography detection method of unique characteristics, as detection of drugs, anaphylactogen, remains of pesticide, tumor markers, cardiac marker and microbial pathogens nucleic acid etc.And follow the related application field to the progressively raising of quantitative detection demand, relevant fluorescent chromatographic detection technique is also arisen at the historic moment.
By existing publicity patent is retrieved, find the patent of patent name " chromatography strip scanning detection method and scanning detector thereof " (application number is 200710037553.4), its scanning detector feature is that optical system comprises xenon source, the ultraviolet lens combination, ultraviolet filter, beam splitter, lens combination, optical filter, photomultiplier, ultraviolet lens and ultraviolet light fulgurite, beam splitter arranges the system middle part, at right-hand ultraviolet lens combination and the xenon source of being provided with from inside to outside of beam splitter, between the lens of ultraviolet lens combination, be provided with ultraviolet filter, left at beam splitter is provided with ultraviolet lens and ultraviolet light fulgurite from inside to outside, above beam splitter, be provided with lens combination and photomultiplier from inside to outside, between the lens of lens combination, be provided with optical filter.
This patent characteristic technical disadvantages is that optical system uses xenon source because the xenon source luminescent spectrum is wideer, so in the optical system simultaneously assemblies such as supporting ultraviolet lens, ultraviolet filter, beam splitter, lens combination as filter, the light splitting purposes.The optical module complexity, cost is higher, equipment volume is bigger.Be subjected to the influence of xenon source service condition and serviceable life simultaneously, its equipment needs supporting suitable temperature and humidity conditions and stabilized power supply, has problems such as start preheating simultaneously.Therefore the clinical emergency treatment detection, the bedside that are not suitable for the chromatography strip detect and field condition detection applications demand.
Retrieval finds that patent name is that " fluorescent quantitation detector " (application number is 201010618601.0) is characterised in that in addition, it comprises excitation source module, photoelectric conversion module, control analysis module and software systems, and described excitation source module comprises the first laser module assembly and the second laser module assembly; The first laser module assembly l comprises excitation source II, spectroscope, and excitation beam A is transmitted in the detection zone of reagent strip through lens, through reflecting fluorescence signal C, shines first photoelectric conversion module through optical filter, lens, grating, imports main circuit then.The first laser module assembly is used for the detection zone emission excitation beam A to reagent strip, through reflecting fluorescence signal C; The second laser module assembly is for the emission of the code area on reagent strip excitation beam B, through reflecting laser signal D; Described photoelectric conversion module is used for receiving fluorescence signal C and the laser signal D of reflection and carries out the photosignal conversion; Described control analysis module comprises main circuit, motor, display screen, and the control analysis module is for the treatment of the electric signal that receives photoelectric conversion module output and handle and output to display screen and show; Described fluorescent quantitation detector is provided with for reagent strip slot and the ID card slot of placing reagent strip and calibration chip id card; This motor is used for driving reagent strip and moves.
This system's shortcoming is: adopt LASER Light Source to excite, have the problem of preheating before the use, optical system need cooperate spectroscope, grating design to reduce parasitic light simultaneously, and the device design is comparatively complicated.
Retrieval finds that patent name is characterized in that for " portable fluorescent chromatographic detection system " (application number is 201010266830.0) in addition, described photoelectricity harvester comprises: LED ultraviolet cold light source, photodiode, optical filtering, two catoptrons and lens, wherein: the ultraviolet light that LED ultraviolet cold light source produces incides first catoptron, the light of first mirror reflects incides lens, the light that lens penetrate incides on the sample, the fluorescence of sample through exciting generation incides optical filtering, the light that optical filtering penetrates incides second catoptron, the light that second catoptron penetrates enters photodiode, the photodiode transmission of electric signals that links to each other with microprocessor.
This Patent Office is sex-limited to be to use the irradiation of ultraviolet LED cold light source, and uses mirror reflects.The ultraviolet spectrum scope is 200-400nm, the ultraviolet LED cold light source does not have best priming effect for most of fluorescent materials, and use mirror design often excitating light strength to be lost, and may cause the low problem of fluorescence probe launching efficiency, thereby cause detection system sensitivity lower.
Therefore the topmost problem of fluorescence detector in the market is, system use inert gas light source, LASER Light Source as the excitation source situation under, need to cooperate opticals filtering/spectrum groupware that exciting light is handled, equipment volume greatly, production cost is higher; Light source life is short, and calibration operation is numerous and diverse when maintenance and use; Portable devices and instant detection demand in the time of can not satisfying on-the-spot the detection.
And use the ultraviolet LED cold light source as excitation source, do not have best priming effect for most of fluorescent materials, the LED light intensity is relatively low simultaneously, under the situation in conjunction with the design of catoptron, excitation light intensity can produce bigger loss, and may cause fluorescence probe to excite degree not enough, thereby cause detection system sensitivity lower.
Summary of the invention
The objective of the invention is to, provide a kind of simple in structure, fluorescent quantitation to detect the portable fluorescence detector sensitive, that degree of accuracy is high.
The technical solution adopted for the present invention to solve the technical problems is: a kind of portable fluorescence detector, comprise the excitation-detection module, pre-amplifying module, the control analysis module, the output terminal of excitation-detection module is connected with the input end of pre-amplifying module, the output terminal of pre-amplifying module is connected with the input end of control analysis module, described excitation-detection module comprises that emission wavelength is the light emitting diode of 400-600nm, lens, crack, filter, photodiode, the corresponding setting with light emitting diode of lens, the light focusing of light emitting diode after the lens refraction is on crack, the corresponding setting with photodiode of filter, the light that excites is received by photodiode through behind the filter.
This arranges and adopts functional narration to limit light emitting diode, lens, crack, filter, the position relation of photodiode, this be because, produce radiative the time at the fluorescence excitation probe, the light that light emitting diode sends is mapped in the crack after the lens refraction, testing sample is in below the crack, the exciting light of each light emitting diode focuses on and concentrates on testing sample detection zone surface formation bar shaped laser spot, the emission light signal that the fluorescence excitation probe produces is behind filter filtering parasitic light, the use photodiode receives, the position of these several parts is corresponding, but do not connect, but light emitting diode, lens, crack, filter, photodiode has constituted special excitation-detection module jointly.
The further setting of the present invention is: also comprise input/output module, storage unit, the control analysis module is connected with input/output module, and storage unit is connected with the control analysis module.
This arranges and has adopted an input/output module and storage unit, storage unit is a plurality of testing results for convenience of the control analysis module, and the effect of input/output module is to expand applicability of the present invention, and simplified structure, this is because the parts that the complete detection system of a cover relates to are very many, in order to simplify one-piece construction, input/output module can dispose the microprinting machine, be used for printing testing result, also can be connected in host computer by data-interface, and realize data management at host computer, storage and printing, and the targeted message by input/output module input, be stored in specific magnetic stripe card, on IC-card or the radio-frequency card, read through supporting Card Reader Unit, like this, the present invention just can be used with various fittings equipment, simplify one-piece construction, reduce equipment volume, be easy to carry.
The further setting of the present invention is: described pre-amplifying module is a pre-amplification circuit.
This arranges and has adopted pre-amplification circuit, and this is because pre-amplification circuit can reduce the generation of undesired signal, and the noise abatement signal is amplified, and the precision of detection is improved, and also makes the present invention sensitiveer.
Said structure utilizes the light emitting diode of 400-600nm, lens, crack, filter, the excitation-detection module fluorescence excitation probe that photodiode is formed produces emission light, and it has been transformed electric signal amplified by pre-amplifying module, because the exciting light of light emitting diode emission is more stable, stimulation effect is stable, pre-amplification circuit then reduces the generation of undesired signal, and the noise abatement signal amplified, sensitivity and the precision of quantitative detection have been improved greatly, excitation portion with respect to prior art, structure is obviously simplified, can be easy to carry, open up extensively range of application of the present invention.
Description of drawings
In order to be illustrated more clearly in the embodiment of the invention or technical scheme of the prior art, to do to introduce simply to the accompanying drawing of required use in embodiment or the description of the Prior Art below, apparently, accompanying drawing in describing below only is some embodiments of the present invention, for those of ordinary skills, under the prerequisite of not paying creative work, can also obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the theory diagram of present embodiment;
Fig. 2 is the structural representation of present embodiment;
Embodiment
With reference to figure 1, Fig. 2 as can be known, a kind of portable fluorescence detector of the present invention, comprise the excitation-detection module, pre-amplifying module, the control analysis module, the output terminal of excitation-detection module is connected with the input end of pre-amplifying module, the output terminal of pre-amplifying module is connected with the input end of control analysis module, the control analysis module respectively with input/output module, storage unit connects, described pre-amplifying module is a pre-amplification circuit, described excitation-detection module comprises that emission wavelength is the light emitting diode 1 of 400-600nm, lens 2, crack 3, filter 4, photodiode 5, light emitting diode 1 be arranged in array (among the array format figure sign), lens 2 are corresponding one by one with light emitting diode 1, the light focusing of light emitting diode 1 after lens 2 refractions is on crack 3, be mapped in the district 6 to be checked and reflect, filter 4 and photodiode 5 corresponding settings, filter 4 is positioned at directly over the crack 3, photodiode 5 is arranged on the filter 4, and the light that excites (fluorescence probe produces emission light) is received by photodiode 5 through filter 4 backs.
The present invention will strengthen exciting light intensity, can arrange to realize by the display of adjusting light emitting diode, and a variety of various combinations are arranged.
The concise and to the point flow process of work of the present invention is: the excitation-detection module uses a plurality of light emitting diodes behind array arrangement, use lens and slit that the exciting light focusing of each light emitting diode is concentrated on testing sample detection zone surface formation bar shaped laser spot, the emission light signal that the fluorescence excitation probe produces is behind filter filtering parasitic light, use the photodiode reception and light signal is converted into electric signal, this electric signal is after pre-amplifying module carries out noise abatement and strengthens processing, enter the control analysis module, the control analysis module is by carrying out computing and contrast the targeted message of input/output module input to signal, finally the section of changing obtains testing result.
Input/output module has a lot of purposes among the present invention, such as being used for sample message, detecting the input of reagent information, instrument/reagent calibration information, and testing result printout, storage, can also carry out other functions expansions by input/output module certainly.
The present invention adopts many photodiodes excited by visible light assembly and the pre-amplification circuit of particular design, it is sensitiveer and accurate that fluorescent quantitation is detected, simplify optical system structure simultaneously, reduce the processing and manufacturing cost, improve light source stability, reduce equipment volume and make it can highly be integrated into the hand held checkout equipment, be more suitable for real-time test field purposes such as emergency treatment check, bedside detection, field test, open up the scope of application of Guangzhou Honda invention.
Obviously, above-described embodiment only be for explanation clearly do for example, and be not restriction to embodiment.To those of ordinary skill in the art, can also make other changes in different forms on the basis of the above description.Here need not also can't give all embodiments exhaustive.And the apparent variation of being extended out thus or change still are in protection scope of the present invention.
Claims (3)
1. portable fluorescence detector, it is characterized in that: comprise the excitation-detection module, pre-amplifying module, the control analysis module, the output terminal of excitation-detection module is connected with the input end of pre-amplifying module, the output terminal of pre-amplifying module is connected with the input end of control analysis module, described excitation-detection module comprises that emission wavelength is the light emitting diode of 400-600nm, lens, crack, filter, photodiode, the corresponding setting with light emitting diode of lens, the light focusing of light emitting diode after the lens refraction is on crack, the corresponding setting with photodiode of filter, the light that excites is received by photodiode through behind the filter.
2. according to the described portable fluorescence detector of claim 1, it is characterized in that: also comprise input/output module, storage unit, the control analysis module is connected with input/output module, and storage unit is connected with the control analysis module.
3. according to claim 1 or 2 described portable fluorescence detectors, it is characterized in that: described pre-amplifying module is a pre-amplification circuit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100869968A CN103196878A (en) | 2013-03-18 | 2013-03-18 | Portable fluorescence detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100869968A CN103196878A (en) | 2013-03-18 | 2013-03-18 | Portable fluorescence detector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103196878A true CN103196878A (en) | 2013-07-10 |
Family
ID=48719570
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013100869968A Pending CN103196878A (en) | 2013-03-18 | 2013-03-18 | Portable fluorescence detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103196878A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109797091A (en) * | 2019-01-17 | 2019-05-24 | 浙江大学 | A kind of rotary quickly dual temperature PCR amplification automatic control device and control method |
| CN112414984A (en) * | 2020-11-06 | 2021-02-26 | 中国计量大学 | Portable milk powder protein content detection box |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1880197A2 (en) * | 2005-04-12 | 2008-01-23 | Caliper Life Sciences, Inc. | Compact optical detection system for a microfluidic devices |
| CN101706434A (en) * | 2009-09-04 | 2010-05-12 | 中国环境科学研究院 | Fluorophototmeter capable of detecting light absorption or light transmission |
| CN101936904A (en) * | 2010-08-31 | 2011-01-05 | 上海交通大学 | Portable fluorescence tomography detection system |
| CN201852788U (en) * | 2010-11-11 | 2011-06-01 | 江阴弘扬传感探测技术有限公司 | Portable fluorescence detector |
| CN102590169A (en) * | 2012-02-21 | 2012-07-18 | 苏州纳诺康生物技术有限公司 | Fluorescent chromatography detector |
| CN102788775A (en) * | 2012-07-31 | 2012-11-21 | 华中科技大学 | Portable high-flux fluorescence detector |
| CN203178190U (en) * | 2013-03-18 | 2013-09-04 | 杭州德安奇生物工程有限公司 | Portable fluorescence detector |
-
2013
- 2013-03-18 CN CN2013100869968A patent/CN103196878A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1880197A2 (en) * | 2005-04-12 | 2008-01-23 | Caliper Life Sciences, Inc. | Compact optical detection system for a microfluidic devices |
| CN101706434A (en) * | 2009-09-04 | 2010-05-12 | 中国环境科学研究院 | Fluorophototmeter capable of detecting light absorption or light transmission |
| CN101936904A (en) * | 2010-08-31 | 2011-01-05 | 上海交通大学 | Portable fluorescence tomography detection system |
| CN201852788U (en) * | 2010-11-11 | 2011-06-01 | 江阴弘扬传感探测技术有限公司 | Portable fluorescence detector |
| CN102590169A (en) * | 2012-02-21 | 2012-07-18 | 苏州纳诺康生物技术有限公司 | Fluorescent chromatography detector |
| CN102788775A (en) * | 2012-07-31 | 2012-11-21 | 华中科技大学 | Portable high-flux fluorescence detector |
| CN203178190U (en) * | 2013-03-18 | 2013-09-04 | 杭州德安奇生物工程有限公司 | Portable fluorescence detector |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109797091A (en) * | 2019-01-17 | 2019-05-24 | 浙江大学 | A kind of rotary quickly dual temperature PCR amplification automatic control device and control method |
| CN112414984A (en) * | 2020-11-06 | 2021-02-26 | 中国计量大学 | Portable milk powder protein content detection box |
| CN112414984B (en) * | 2020-11-06 | 2023-04-25 | 中国计量大学 | Portable milk powder protein content detection box |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102590169A (en) | Fluorescent chromatography detector | |
| US7708944B1 (en) | Ultra-sensitive, portable capillary sensor | |
| US9476879B2 (en) | Test strip detection system | |
| CN201653906U (en) | Biological immunochromatography chip detector | |
| CN107709975B (en) | Fluorescence detection method and system | |
| AU2016301228B2 (en) | Multi excitation-multi emission fluorometer for multiparameter water quality monitoring | |
| US20180188152A1 (en) | Radiation Carrier and Use Thereof in an Optical Sensor | |
| US8542349B2 (en) | Arrangement and a method for controlling a measurement head of an optical measurement instrument | |
| CN101706435B (en) | Device and method for detecting biomarker | |
| US20100197515A1 (en) | Detecting target molecules in a sample | |
| WO2005106433A1 (en) | Optical information reader | |
| JP2005515405A (en) | Microarray imaging using optical fiber exciter | |
| CN105637349A (en) | Series absorbance glass and interference filters | |
| CN110487767B (en) | Portable up-conversion fluorescence detector | |
| US20200080941A1 (en) | Multi excitation-multi emission fluorometer for multiparameter water quality monitoring | |
| CN103196878A (en) | Portable fluorescence detector | |
| CN203178190U (en) | Portable fluorescence detector | |
| JP2006058276A (en) | Automated system and sample analyzing method | |
| CN110100315A (en) | Charge avalanche photodetector system | |
| CN113189065B (en) | Optical detection method | |
| CN101317085A (en) | Bio chip device with a sample compartment and a light sensitive element, method for the detection of fluorescent particles within at least one sample compartment of a bio chip device | |
| CN100468047C (en) | Spectrum microsensor for integrated biochemical chip | |
| CN210269617U (en) | Optical detection device and capillary electrophoresis apparatus | |
| CN2938079Y (en) | Integrated spectral micro-sensor element | |
| CN103616359A (en) | Fluorescent quantitative detection method for green fluorescent protein (GFP) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130710 |