CN103196855A - Method for measuring trace element lead in linden tree by flame atomic absorption spectrometry (FAAS) - Google Patents
Method for measuring trace element lead in linden tree by flame atomic absorption spectrometry (FAAS) Download PDFInfo
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- CN103196855A CN103196855A CN 201310130792 CN201310130792A CN103196855A CN 103196855 A CN103196855 A CN 103196855A CN 201310130792 CN201310130792 CN 201310130792 CN 201310130792 A CN201310130792 A CN 201310130792A CN 103196855 A CN103196855 A CN 103196855A
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Abstract
The invention discloses a method for measuring the content of lead in a linden tree by flame atomic absorption spectrometry (FAAS). A sample treatment method comprises the following steps of: accurately weighing a sample and digesting the sample for a plurality of times; transferring digestive juice and washing liquid into a 50mL volumetric flask together; and fixing the volume of the solution to be measured by secondary deionized water. The light absorption value of the sample is measured under the following conditions of the FAAS: the wavelength is 270.0 lambda/nm, the negative high voltage is 389V, the reference current is 5mA, the actual current is 2.5MA, the height of a combustor is 8.6h/mm, the spectral band-width is 0.5SBW/n, the air flow rate is 1.1L/min, and the acetylene flow rate is 1.1L/min. The content of the sample is calculated according to a regression curve A=0.0286C+0.0034. The measurement method is simple and rapid in operation and high in flexibility, and is also used for measuring the content of trace element lead in other traditional Chinese medicines.
Description
Technical field
The present invention relates to the method for micro-lead content in the flame atom spectrum measuring lime tree.
Background technology
The sweet temperature of its property of Mel can be built up health and be improved mood, reduces central nervous excitation, safeguards function of brain cell.Will and spirit is mended stomach, the detoxifcation that relieves the pain, and the effect of the multiple disease for the treatment of and alleviating hypertension, heart disease, constipation, insomnia is arranged.Tilia amurensis claims seed linden, leaflet linden, Amur linden, lime tree again, and for China originates in seeds, reaching the mountain region, North China northeastward all has wild distribution.The vertical distribution on ground such as the Changbai Mountain of the Northeast, the Xiaoxinanlin Mountains can reach height above sea level more than 1100 meters.The chaff linden cries Tilia platyphyllos, distant linden, bodhi tree again, is distributed in China Heilungkiang and two province forest zones, Jilin in a large number.Mel is that honeybee is gathered from Flores tiliae.Be white in color, special light amber.The fragrant odour strong fragrance.Taste is sweet agreeable to the taste, and easily crystallization is fine and smooth pure white grease-like after the crystallization, is first-class honey, and the masses are called superfine honey, are subjected to domestic and international welcome deeply.Its sparkling and crystal-clear pure white, colour purity flavor perfume, nutritious, contain glucose and tartaric acid more than 70%, also have multivitamin and inorganic salts, organic acid enzyme, the biotin that promotes people's bulk-growth and vigor is arranged, can improve health.It is not only the complete food of nutrition, can also treat cough, bronchitis, and makes to sleep peacefully sedative.
Trace element is relevant with pharmacology, and trace element is the essential material of human body normal life institute, and very important effect is arranged in organization and physiology course.If will causing multiple disease unusually, body trace element content takes place.The flame atomic absorption spectrometry of measuring element calcium in the lime tree has been explored in this research, for the mensuration of lead content in the Chinese crude drug provides a kind of method.While also provides scientific basis for further research and the development and use of lime tree and Mel.
Summary of the invention
The invention provides a kind ofly at trace element lead Determination on content method in the lime tree, the main process of this method is: 1, sample preparation; 2, the condition of work of flame atomic absorption method the best; 3, the content of lead in the bioassay standard sample; 4, draw typical curve and draw typical curve regression equation in the finite concentration scope; 5, working sample; 6, calculate the content of trace element lead in the lime tree sample.
The flame atomic absorption spectrometry method of trace element lead in the lime tree of the present invention, formed by following steps:
The processing of sample:
Accurately take by weighing sample, divide inferior in the small beaker, add nitric acid, soaked overnight, add surface plate at small beaker next day, adds nitric acid, place on the electric hot plate and heat, make treatments of the sample complete, add a certain amount of perchloric acid, digest to light brown gas and emit to the greatest extent, sample liquid near 2mL about, take off cooling, with secondary deionized water flushing small beaker inwall and surface plate, sample liquid and cleansing solution are transferred in the 50mL volumetric flask in the lump, use the secondary deionized water constant volume, and be to be measured.
NITRATE BY FLAME ATOMIC absorbs best condition of work:
Wavelength is 270.0 λ/nm; Negative high voltage 389V; Reference electric current 5mA; Actual current 2.5mA; Burner height 8.6h/mm; Air mass flow 1.1L/min; Acetylene flow 1.1L/min.
Draw typical curve and draw the typical curve regression equation:
Pb standard stock solution dilution preparation Pb mass concentration with 1000 μ g/mL: 0.0000,0.5000,1.0000,3.0000,5.0000,10.0000 μ g/mL, press the Atomic Absorption Spectrometry condition, the light absorption value of bioassay standard series solution is drawn typical curve and is drawn regression equation according to measurement result respectively.The typical curve regression equation A (light absorption value) of trace element lead content C (μ g/mL) is A=0.0286C+0.0034, R
2The concentration range of linearity that is 0.9989 lead content is 0.5000～10.0000 μ g/mL.
The mensuration of sample:
Determine the light absorption value of lime tree sample by the flame atom spectrum measuring condition.
Calculate micro-lead content in the lime tree sample:
By the light absorption value of the lime tree sample that records, calculate the content of sample lime tree trace element lead according to regression curve.
Description of drawings
Fig. 1 is absorbance (A) and lead content (C) relation curve.
Embodiment
Accurately take by weighing sample, divide inferior in the small beaker, add nitric acid, soaked overnight, add surface plate at small beaker next day, adds nitric acid, place on the electric hot plate and heat, make treatments of the sample complete, add a certain amount of perchloric acid, digest to light brown gas and emit to the greatest extent, sample liquid near 2mL about, take off cooling, with secondary deionized water flushing small beaker inwall and surface plate, sample liquid and cleansing solution are transferred in the 50mL volumetric flask in the lump, use the secondary deionized water constant volume, and be to be measured.
NITRATE BY FLAME ATOMIC absorbs best condition of work:
Wavelength is 270.0 λ/nm; Negative high voltage 389V; Reference electric current 5mA; Actual current 2.5mA; Burner height 8.6h/mm; Air mass flow 1.1L/min; Acetylene flow 1.1L/min.
The typical curve regression equation:
The typical curve regression equation A (light absorption value) of trace element lead content C (μ g/mL) is A=0.0286C+0.0034, R
2Be 0.9989, the concentration range of linearity of lead content is 0.5000～10.0000 μ g/mL.
The mensuration of sample:
Determine the light absorption value of lime tree sample by the flame atom spectrum measuring condition.
Calculate micro-lead content in the lime tree sample:
By the lime tree sample light absorption value that records, go out the content of sample lime tree trace element lead according to the typical curve regression equation calculation.
Claims (3)
1. accurately take by weighing sample, divide inferior in the small beaker, add nitric acid, soaked overnight, add surface plate at small beaker next day, adds nitric acid, place on the electric hot plate and heat, make treatments of the sample complete, add a certain amount of perchloric acid, digest to light brown gas and emit to the greatest extent, sample liquid near 2mL about, take off cooling, with secondary deionized water flushing small beaker inwall and surface plate, sample liquid and cleansing solution are transferred in the 50mL volumetric flask in the lump, use the secondary deionized water constant volume, and be to be measured.
2. flame atom spectrum measuring condition: wavelength is 270.0 λ/nm; Negative high voltage 389V; Reference electric current 5mA; Actual current 2.5mA; Burner height 8.6h/mm; Air mass flow 1.1L/min; Acetylene flow 1.1L/min.
3. the typical curve regression equation A (light absorption value) of micro-lead content C (μ g/mL) is A=0.0286C+0.0034, R
2Be 0.9989, the concentration range of linearity of lead content is 0.5000～10.0000 μ g/mL.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201310130792 CN103196855A (en) | 2013-04-16 | 2013-04-16 | Method for measuring trace element lead in linden tree by flame atomic absorption spectrometry (FAAS) |
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| CN 201310130792 CN103196855A (en) | 2013-04-16 | 2013-04-16 | Method for measuring trace element lead in linden tree by flame atomic absorption spectrometry (FAAS) |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103512854A (en) * | 2013-09-23 | 2014-01-15 | 桂林理工大学 | Flame atomic absorption spectrometry method for separating, enriching and detecting trace plumbum in water sample by utilizing thiobarbituric-acid-modified silica gel |
| CN103528980A (en) * | 2013-09-23 | 2014-01-22 | 桂林理工大学 | Flame atomic absorption spectrum method for separating, enriching and determining trace lead and cadmium in water sample by using thiomalamic acid modified silica gel |
| CN104713873A (en) * | 2013-12-11 | 2015-06-17 | 江南大学 | Trace lead spectrophotometric measuring method |
-
2013
- 2013-04-16 CN CN 201310130792 patent/CN103196855A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103512854A (en) * | 2013-09-23 | 2014-01-15 | 桂林理工大学 | Flame atomic absorption spectrometry method for separating, enriching and detecting trace plumbum in water sample by utilizing thiobarbituric-acid-modified silica gel |
| CN103528980A (en) * | 2013-09-23 | 2014-01-22 | 桂林理工大学 | Flame atomic absorption spectrum method for separating, enriching and determining trace lead and cadmium in water sample by using thiomalamic acid modified silica gel |
| CN103512854B (en) * | 2013-09-23 | 2015-06-17 | 桂林理工大学 | Flame atomic absorption spectrometry method for separating, enriching and detecting trace plumbum in water sample by utilizing thiobarbituric-acid-modified silica gel |
| CN103528980B (en) * | 2013-09-23 | 2015-12-09 | 桂林理工大学 | Utilize the flame atomic absorption spectrometry method of trace lead, cadmium in thiomalic acid modified silica-gel separation and concentration-mensuration water sample |
| CN104713873A (en) * | 2013-12-11 | 2015-06-17 | 江南大学 | Trace lead spectrophotometric measuring method |
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Application publication date: 20130710 |