CN103191679A - Method for preparing microscale hydrogel - Google Patents
Method for preparing microscale hydrogel Download PDFInfo
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- CN103191679A CN103191679A CN2013100784739A CN201310078473A CN103191679A CN 103191679 A CN103191679 A CN 103191679A CN 2013100784739 A CN2013100784739 A CN 2013100784739A CN 201310078473 A CN201310078473 A CN 201310078473A CN 103191679 A CN103191679 A CN 103191679A
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Abstract
The invention provides a method for preparing microscale hydrogel, including 1) injecting a hydrogel precursor into a penholder of a sample adding pen, wherein the sample adding pen also includes a pen tip, the front end of the pen tip is provided with a sample discharging hole, the sample discharging hole is provided with a rotatable bead, the rear end of the pen tip is communicated with the penholer, and the hydrogel precursor is injected into the penholder and then sealed with sealing oil; 2) writing the hydrogel precursor by the sample adding pen on a substrate; and 3) performing curing process for the hydrogel precursor on the substrate. According to the present invention, the sample adding pen is used for writing the trace hydrogel precursor on the substrate and then the hydrogel precursor is cross-linked and fixed to form the microscale hydrogel. The method has the characteristics of high material utilization rate, low cost, easy and flexible operation, and high processing controllability.
Description
Technical field
The invention belongs to biological technology application, be specifically related to a kind of simple and easy, preparation method of minute yardstick hydrogel cheaply that operates.
Background technology
Hydrogel is the macromolecule network structure of moisture content superelevation (being generally more than 90%), has characteristics such as good biocompatibility, intelligence, is widely used in fields such as biology, medical science.Microgel refers to the hydrogel material (being generally micron, Nano grade) in the minute yardstick scope.Microgel can be used for medicine carrying because size is little and be porous network structure, in medical domain as the pharmaceutical carrier of slowly-releasing, target.Simultaneously, because its good biocompatibility also can load cell, as " biological brick " in the organizational project, make up tissue.
The means of preparation microgel mainly are photomask method, micro-fluidic method and cell Method of printing at present.Wherein, the photomask method is the method for the most ripe and extensive use, can be used for making the microgel of different shape and yardstick, yet this method only is applicable to the gel rubber material of photo-crosslinking, has seriously limited the application of this method.Micro-fluidic method and cell impact system can be used for the preparing gel of temperature crosslink.Yet these three kinds of methods all relate to complicated and tediously long operating process and expensive equipment, cost of manufacture height.Therefore, simple to operate, method flexibly, have wide range of applications low-cost minute yardstick gel process for preparing significant to the extensive use of microgel.
Summary of the invention
The object of the present invention is to provide a kind of easy and simple to handle, flexibly, the preparation method of minute yardstick hydrogel cheaply.
For achieving the above object, the present invention has adopted following technical scheme:
1) hydrogel precursor is injected the penholder of application of sample pen, described application of sample pen also comprises nib, the front end of nib offers sample flow and portals, in portalling, sample flow is provided with rotary ball, the rear end of nib is connected with penholder, after injecting hydrogel precursor, with Seal Oil hydrogel precursor is sealed in the penholder;
2) with the application of sample pen hydrogel precursor is write in substrate;
3) suprabasil hydrogel precursor is cured processing.
Described application of sample pen also comprises for connecting the jockey of nib with penholder.
Described writing refers to that with end in contact substrate before the nib it is mobile in substrate to drive nib then, and the ball of written front end is rotated, and the hydrogel precursor that drives in the penholder flows out, and the hydrogel precursor of outflow is coated in the substrate.
The diameter of described ball is 100nm-1cm.
The application of sample weight range of described application of sample pen is 10-1000nL/cm.
Described curing is handled and is comprised ultraviolet lighting, intensification or cooling.
Beneficial effect of the present invention is:
The present invention utilizes the application of sample pen to write the minor amount of water gel precursors in substrate, makes hydrogel precursor crosslinked fixing then, forms the minute yardstick hydrogel, and this method has stock utilization height, with low cost, easy to use flexible, characteristics such as processing controllability height.
Description of drawings
Fig. 1 is the structural representation of application of sample pen of the present invention; Among Fig. 1: 1 nib, 2 penholders, 3 balls, 4 jockeys, 5 hydrogel precursors, 6 Seal Oil;
Fig. 2 is the wire polyethylene glycol hydrogel effect (scale is 500 μ m among the figure) of the present invention's preparation;
Fig. 3 is the effect (scale is 500 μ m among the figure) of the point-like temperature crosslink gelatin minute yardstick hydrogel of the present invention's preparation;
Fig. 4 is that an application of sample of the present invention application of sample amount is with the variation of writing speed;
Fig. 5 is that an application of sample of the present invention application of sample amount is with the variation of front end ball size;
Fig. 6 is that an application of sample of the present invention application of sample amount is with the variation of penholder inner stuffing (hydrogel precursor) height.
The specific embodiment
The invention will be further described below in conjunction with drawings and Examples.
The preparation method of minute yardstick hydrogel of the present invention, may further comprise the steps: the penholder 2 that 1) hydrogel precursor is injected the application of sample pen, described application of sample pen also comprises nib 1, the front end of nib 1 offers sample flow and portals, in portalling, sample flow is provided with rotary ball 3, the rear end of nib 1 is connected with penholder 2, after injecting hydrogel precursor, with Seal Oil 6(mineral oil) hydrogel precursor is sealed in the penholder 2, described application of sample pen also comprises for connecting the jockey 4 of nib 1 with penholder 2, referring to Fig. 1; 2) with the application of sample pen hydrogel precursor is write in substrate (as glass plate); Described writing refers to that with end in contact substrate before the nib 1 it is mobile in substrate to drive nib 1 then, and the ball 3 of written 1 front end is rotated, and the hydrogel precursor that drives in the penholder 2 flows out, and the hydrogel precursor of outflow is coated in the substrate, can realize micro sample-adding; 3) suprabasil hydrogel precursor being cured processing makes suprabasil hydrogel precursor crosslinked fixing; Described curing is handled and is comprised ultraviolet lighting, intensification or cooling.
The diameter of described ball 3 is 100nm-1cm.The application of sample weight range of described application of sample pen is 10-1000nL/cm.By adjusting the application of sample amount of hydrogel precursor height (Fig. 6) change application of sample pen in writing speed (Fig. 4), ball diameter (Fig. 5), base material or the penholder, writing speed refers to that written front end is in the mobile speed of substrate, writing speed can be controlled by driving mechanism, simultaneously, can connect the hydrogel precursor supplementary mechanism in the penholder rear end, make the stable height of hydrogel precursor maintenance in the penholder.
Embodiment
The structure of biological application of sample pen as shown in Figure 1, formed by 6 parts, nib 1 mainly provides the support point of ball 3, penholder 2 is used for storing hydrogel precursor, ball 3 provides enough shearing forces during frictional rotation in substrate, drive hydrogel precursor 5 and flow out, jockey 4 is used for connecting penholder 2 and nib 1, and Seal Oil 6 prevents sample (hydrogel precursor) volatilization.When biological application of sample pen (is similar to the writing process of ball pen or gel ink pen) when substrate is write, the ball 3 of written 1 front end rotates, and drives hydrogel precursor 5 and flows out.
Utilize biological application of sample pen to prepare hydrogel, may further comprise the steps:
Step 1: an amount of required process water gel precursors (10 μ L-2mL) is injected penholder, use mineral oil (Seal Oil) sealing thereafter, avoid evaporating.
Step 2: hold this biological application of sample pen and write at glass plate, make the preceding end in contact substrate of nib, it is mobile in substrate to drive nib then, the ball of written front end is rotated, the drive hydrogel precursor flows out, the hydrogel precursor that flows out is coated in the substrate, and hydrogel precursor can be write into difformities such as point-like, wire flexibly.
Step 3: utilize chemical reaction to make suprabasil hydrogel precursor crosslinked fixing (according to the hydrogel kind and difference, as ultraviolet lighting, intensification or cooling etc.), then can form the minute yardstick hydrogel of different shape.
Example 1 utilizes biological application of sample pen to prepare photo-crosslinking polyethylene glycol minute yardstick hydrogel
Step 1: 200 μ L polyethylene glycol hydrogel precursors are injected penholder, with the mineral oil sealing, avoid evaporating thereafter.Polyethylene glycol hydrogel precursor composed as follows: 10% molecular weight is 1000 polyethylene glycol, 0.5% 2-hydroxy-2-methyl-1-phenyl-1-acetone, and surplus is water.
Step 2: hold this biological application of sample pen and write point-like at glass plate.
Step 3: utilized ultraviolet source irradiation 30 seconds, and made suprabasil hydrogel precursor crosslinked fixing, then form the minute yardstick polyethylene glycol hydrogel (Fig. 2) of point-like.
Example 2 is utilized a biological application of sample preparation temperature cross-linked gelatin minute yardstick hydrogel
Step 1: 200 μ L gelatin precursors are injected penholder, with the mineral oil sealing, avoid evaporating thereafter, the gelatin precursor is 3% the pigskin gelatin aqueous solution.
Step 2: hold this biological application of sample pen and write wire at glass plate.
Step 3: be cooled to 4 degrees centigrade and make suprabasil hydrogel precursor crosslinked, then can form the minute yardstick hydrogel (Fig. 3) of wire.
Claims (6)
1. the preparation method of a minute yardstick hydrogel is characterized in that, may further comprise the steps:
1) hydrogel precursor is injected the penholder (2) of application of sample pen, described application of sample pen also comprises nib (1), the front end of nib (1) offers sample flow and portals, in portalling, sample flow is provided with rotary ball (3), the rear end of nib (1) is connected with penholder (2), after injecting hydrogel precursor, with Seal Oil (6) hydrogel precursor is sealed in the penholder (2);
2) with the application of sample pen hydrogel precursor is write in substrate;
3) suprabasil hydrogel precursor is cured processing.
2. according to the preparation method of the described a kind of minute yardstick hydrogel of claim 1, it is characterized in that: described application of sample pen also comprises for connecting the jockey (4) of nib (1) with penholder (2).
3. according to the preparation method of the described a kind of minute yardstick hydrogel of claim 1, it is characterized in that: described writing refers to the preceding end in contact substrate of nib (1), it is mobile in substrate to drive nib (1) then, the ball (3) of nib (1) front end is rotated, the hydrogel precursor that drives in the penholder (1) flows out, and the hydrogel precursor of outflow is coated in the substrate.
4. according to the preparation method of the described a kind of minute yardstick hydrogel of claim 1, it is characterized in that: the diameter of described ball (3) is 100nm-1cm.
5. according to the preparation method of the described a kind of minute yardstick hydrogel of claim 1, it is characterized in that: the application of sample weight range of described application of sample pen is 10-1000nL/cm.
6. according to the preparation method of the described a kind of minute yardstick hydrogel of claim 1, it is characterized in that: described curing is handled and is comprised ultraviolet lighting, intensification or cooling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310078473.9A CN103191679B (en) | 2013-03-12 | 2013-03-12 | A kind of preparation method of microscale hydrogel |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310078473.9A CN103191679B (en) | 2013-03-12 | 2013-03-12 | A kind of preparation method of microscale hydrogel |
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| Publication Number | Publication Date |
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| CN103191679A true CN103191679A (en) | 2013-07-10 |
| CN103191679B CN103191679B (en) | 2015-10-28 |
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| CN201310078473.9A Expired - Fee Related CN103191679B (en) | 2013-03-12 | 2013-03-12 | A kind of preparation method of microscale hydrogel |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1874902A (en) * | 2003-11-07 | 2006-12-06 | 樱花彩色产品株式会社 | Coating tool |
| CN201782232U (en) * | 2010-07-27 | 2011-04-06 | 雅蒂斯企业有限公司 | Daubing pen structure |
| CN102209640A (en) * | 2008-11-11 | 2011-10-05 | 株式会社樱花彩色笔 | Applicator tip and applicator |
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- 2013-03-12 CN CN201310078473.9A patent/CN103191679B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1874902A (en) * | 2003-11-07 | 2006-12-06 | 樱花彩色产品株式会社 | Coating tool |
| CN102209640A (en) * | 2008-11-11 | 2011-10-05 | 株式会社樱花彩色笔 | Applicator tip and applicator |
| CN201782232U (en) * | 2010-07-27 | 2011-04-06 | 雅蒂斯企业有限公司 | Daubing pen structure |
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| CN103191679B (en) | 2015-10-28 |
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Granted publication date: 20151028 Termination date: 20180312 |