CN103190621B - Propolis soft capsule and preparation method thereof - Google Patents
Propolis soft capsule and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a propolis soft capsule and a preparation method thereof. The preparation method comprises the following steps: injecting glycerin and pure water into an aerosol can, stirring and heating to 60-70 DEG C, adding gelatin, continuing to stir for 20-30min, performing vacuum defoaming to prepare a capsule body; firstly, grinding propolis and then fully mixing polyethylene glycol and glycerol for 20-40min at 50-70 DEG C, then adding folium ginkgo extract, fully mixing for 30-60min, performing milling for one to three times by a colloid mill to obtain evenly mixed content; and performing pelleting, shaping, pellet washing, drying, pellet sorting and subpackaging to the content and the capsule body, to prepare the propolis soft capsule. Compared with the prior art, the soft capsule has the advantages of being good in bioavailability, excellent in drug stability, capable of covering unpleasant odor, accurate in dosage, sealed, safe, convenient to carry and use, and the like, and the propolis soft capsule has the function of increasing the immunity function.
Description
Technical field
The present invention relates to technical field of health care food, especially relate to a kind of bee glue soft capsule and preparation method thereof.
Background technology
Immunity refers to a series of intrinsic protective system that body is set up for a long time, and develop immunitypty is healthy to guarantee is very important, and the reduction of body immunity can cause various disease.Immunity has three kinds of functions to body: 1, defense function; 2, Selfstabilizing function; Function is inspected in 3 immunity.By these functions to maintain the balance of vivo environment and to stablize, then can there is immunity disease in immunologic dysfunction, even tumour occurs.
At present, the health food of develop immunitypty through the Ministry of Public Health and State Food and Drug Administration's approval has thousands of, has the function factor improving immunity function and has polysaccharide, flavonoids, saponins, protein etc.
Enriching and the bioactivator of uniqueness contained by propolis, make it have antibacterial, anti-inflammatory, antipruritic, anti-oxidant, strengthen immune, hypoglycemic, reducing blood lipid, the several functions such as antitumor, medical treatment, health-care effect is widely had to human body, now become the focus of various countries' scientific research, and become emerging health products and extremely praise highly.
Summary of the invention
Object of the present invention be exactly provide that a kind of bioavilability is good to overcome defect that above-mentioned prior art exists, medicine stability is good, the bee glue soft capsule with develop immunitypty function and preparation method thereof.
Object of the present invention can be achieved through the following technical solutions:
A kind of bee glue soft capsule, the content comprising utricule and enclose in utricule, described content is made up of the raw material of following component and weight percent content:
Described content is made up of the raw material of following component and weight percent content:
General flavone content >=18wt% in described propolis.
General flavone containing 24wt% in described ginkgo biloba p.e.
A preparation method for bee glue soft capsule, the method comprises the following steps:
(1) make utricule: with gelatin, glycerine and pure water for utricule raw material, glycerine and pure water are injected in glue pot, is heated with stirring to 60 ~ 70 DEG C, then adds gelatin, continue to stir final vacuum deaeration in 20-30 minute, make and obtain utricule;
(2) prepare content: after first propolis being pulverized and PEG400 and glycerine at 50 ~ 70 DEG C, fully mix 20 ~ 40min, and then add ginkgo biloba p.e and fully mix 30 ~ 60min, cross colloid mill 1 ~ 3 time, obtain the content mixed;
(3) prepare capsule: the content that step (2) is prepared and step (1) make obtain utricule through pelleting, shape, wash ball, drying, pick ball and packing step, prepare bee glue soft capsule.
In step (1), the weight ratio of gelatin, glycerine and pure water is 5 ~ 20: 1 ~ 10: 5 ~ 20.
The propolis that the present invention adopts, the main active of ginkgo biloba p.e are all flavonoids, have the health-care effect of develop immunitypty.
Propolis composition is quite complicated, main component is flavone compound, also have the multiple compounds such as phenols, alcohols, acids lipid, in addition also containing trace element such as a small amount of iron, calcium, copper, silicon, manganese, lead, tin etc. and vitamin A, Cobastab, several amino acids, enzyme, forulic acid etc.Propolis or propolis extract are pharmaceutically being used as anaphylactogen, radioresistance, antitumor, immune response stimulating etc.
The immunological enhancement of propolis is inseparable with the physical behavior of the chemical composition of its complexity and its extract.VA in propolis is a kind of centrally acting adjuvant, works to immune maincenter, can stimulate the enhancing of antibody response, and VB can strengthen humoral immunity, strengthens antibody amount.Zn in propolis can excite the immunity of animal.Immunocyte is in DNA building-up process, several dependence metalloenzyme control the Reproduction of cell, lack Zn and very large infringement is had to the lymphocytic immunity function of T, phagocyte significant change will be caused, lose its polymorphism and pseudopodium and become level and smooth round cell thus immunity function is declined.A large amount of experiments also proves, the many enzymes in propolis, alcohols, lipid, the immune system of acid to body have and act on widely.Pinocembrin in propolis, Galangin, Kaempferol, there is antibacterial activity to tonka-bean benzoate, caffeic acid fat and propolis extract, itself does not have antigenicity, but can immunoadjuvant function be played, the generation of energy enhancing antibody, the content of total serum protein and gamma globulin is increased, the phagocytic activity of leucocyte and macrophage strengthens, and the specificity of body and non-specific immunity strengthen, and the immunological enhancement of propolis is the synergistic result of above all materials.
Ginkgo biloba p.e comprises ginkgolides and flavonoids (based on Quercetin, kaempferia galamga rope, bigcatkin willow syphilis), except having the effects such as expansion cardiovascular and cerebrovascular, diastole bronchus, antibacterial, anti-inflammatory, also has humidification to immune function of human body.
Propolis can be partly dissolved in PEG400, is decentralized medium with PEG400, is conducive to propolis and ginkgo biloba p.e suspendible in medium even, is conducive to raw material and disperses in alimentary canal, be easy to absorb.
Compared with prior art, soft capsule of the present invention has that bioavilability is good, medicine stability is good, cover bad smell, dosage accurately, sealing, safely, to carry and the advantage such as easy to use, bee glue soft capsule of the present invention has the effect of develop immunitypty function.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1
A kind of bee glue soft capsule, the content comprising utricule and enclose in utricule, content is made up of the raw material of following component and weight percent content: propolis 50, ginkgo biloba p.e 5, PEG400 40, glycerine 5; Wherein, the general flavone content >=18wt% in propolis, containing 24wt% general flavone in ginkgo biloba p.e.
A preparation method for bee glue soft capsule, the method comprises the following steps:
(1) make utricule: with gelatin, glycerine and pure water for utricule raw material, glycerine and pure water are injected in glue pot, is heated with stirring to 60 DEG C, add gelatin again, continue stirring final vacuum deaeration in 30 minutes, make and obtain utricule, wherein the weight ratio of gelatin, glycerine and pure water is 5: 10: 20;
(2) prepare content: after first propolis being pulverized and PEG400 and glycerine at 50 ~ 70 DEG C, fully mix 20 ~ 40min, and then add ginkgo biloba p.e and fully mix 30 ~ 60min, cross colloid mill 1 ~ 3 time, obtain the content mixed;
(3) prepare capsule: the content that step (2) is prepared and step (1) make obtain utricule through pelleting, shape, wash ball, drying, pick ball and packing step, prepare bee glue soft capsule, every capsules is 500 milligrams containing content.
The develop immunitypty function of bee glue soft capsule prepared by the present embodiment is detected.
1, sample: bee glue soft capsule prepared by the present embodiment
2, experimental animal and environment: SPF Kun Ming mice, credit number: SCXK (Shanghai) 2002-0010, body weight 18 ~ 22 grams, female 100, male 100, be divided into large group of four immunity, every large group 50, the mouse that each immunity is organized greatly is divided into water control group, solvent control group, basic, normal, high dosage group, each 10.Immunity one group (female mice 50) is for delayed allergy (DTH) experiment, the mensuration of serum hemolysin, the mensuration of antibody-producting cell number; Immunity two groups (male mices 50) is tested for mouse carbonic clearance; Immunity three groups (female mice 50) engulfs chicken red blood cell experiment for Turnover of Mouse Peritoneal Macrophages; Immunity four groups (male mice 50) measures for mouse lymphocyte transformation experiment and NK cells in mice activity experiment.Experimental situation temperature 22 ± 2 DEG C, relative humidity 50% ± 10%.
3, dose design and tested material give mode: bee glue soft capsule of the present invention is 1.0g/60kg body weight every day to the RD of human body, be equivalent to 0.0167g/kg.BW, the low middle high Three doses of mouse is determined by 5,10,30 multiple doses being equivalent to human body RD, in the present embodiment, respectively 0.083g/kg.BW is set to the dosage of mouse, 0.167g/kg.BW, 0.50g/kg.BW tri-kinds, per os once a day gavage gives mouse tested material, and gavage volume presses 10ml/kg.BW.Tested material prepared by the front corn oil of gavage, in basic, normal, high dosage group, the concentration of tested material is respectively 8.33g/L, 16.667g/L and 50.00g/L, negative control group replaces tested material with isopyknic distilled water, solvent control group replaces tested material with isopyknic corn oil, tests every immune indexes after 30 days.Mouse feeds with outbred mice special feed and raises.
4, experimental technique
4.1 organ weight ratio pH-value determination pHs
After mouse weights, cervical dislocation is put to death, and gets its thymus gland, spleen, removes most manadesma, blot organ surface blood stains and weigh with filter paper, calculates thymus gland body weight ratio and spleen weight ratio.The data obtained PEMS statistical software carries out variance analysis.
4.2 delayed allergies (the sufficient sole of the foot thickens method) (DTH)
Get sheep blood, with brine three times, every mouse is through lumbar injection 2% (v/v, configure with physiological saline) hematocrit SRBC (2000r/min, 10min) 0.2ml, after sensitization 4 days, measure the sufficient sole of the foot thickness in left and right, same position measures 3 times, average, then at measuring point hypodermic injection 20% (v/v configures with physiological saline) hematocrit SRBC20 μ l, inject and measure the sufficient sole of the foot thickness in left and right in latter 24 hours, represent the degree of DTH with the difference of attacking the sufficient sole of the foot thickness in front and back.The data obtained is measurement data, variance analysis is carried out with SPSS statistical software, if tested material attacks the difference of the sufficient sole of the foot thickness in front and back higher than control group, and difference has conspicuousness (P < 0.05), can conclude that this tested material is improved the effect of mouse delayed allergy ability.
The mouse spleen lymphocyte conversion test (mtt assay) of 4.3ConA induction
Animal gives sample after 30 days continuously, cervical dislocation is put to death, get spleen, make splenocyte suspension, adjustment cell concentration is 3 × 106/ml, is divided into by cell suspension holes to add 24 well culture plate China, every hole 1ml, one hole adds 75 μ lConA liquid (being equivalent to 7.5 μ g/ml), and 5%CO in contrast, is put in another hole
2, cultivate 68h for 37 DEG C.Cultivation terminates front 4h, every hole gentle aspiration supernatant 0.7ml, add 0.7ml not containing the PRMI1640 nutrient solution of calf serum, add MTT (5mg/ml) 50 μ l/ hole simultaneously, continue to cultivate 4h, after cultivation terminates, every hole adds 1ml acid isopropyl alcohol, piping and druming mixing, after purple crystal is dissolved completely, then moves into liquid in 96 orifice plates, every hole adds 3 Duplicate Samples, carry out measurement absorbance (A value) with ELIASA at 570nm wavelength, calculate the difference of test hole A value and control wells A value, to represent lymphocytic competence for added value.Result variance analysis is analyzed.If the lymphocytic multiplication capacity of tested material group is higher than control group, and difference has conspicuousness, can judge that this tested material is improved the effect of the mouse spleen lymphocyte conversion capability of ConA induction.
4.4 antibody-producting cells measure (Jerne improves slide method)
Animal gives sample after 30 days continuously, and by defiber Mianyang erythrocyte immune after 5 days, animal cervical dislocation is put to death, and takes out spleen, makes splenocyte suspension, and adjustment cell concentration is 5 × 10
6individual/ml.After top layer culture medium (1g agarose adds distilled water 100ml) heating for dissolving, put into 45 DEG C of water bath heat preservations, mix with the Hanks liquid of equivalent pH7.2-7.4,2 times of concentration, packing small test tube, often pipe 0.5ml, 50 μ l10%SRBC are added again in pipe, 20 μ l splenocyte suspensions, mix rapidly, are poured on the 6cm plate of brush thin agar layer, after continuing incubation 1.5h, counting hemolysis plaque number.Result variance analysis is added up.If the lymphocytic multiplication capacity of tested material group is higher than control group, and difference has conspicuousness, can judge that this tested material has the effect strengthening mouse antibodies cell number.
4.5 mice serum hemolysin tests:
Get sheep blood, with brine 3 times, every mouse (configures with physiological saline through lumbar injection 2%, volume ratio) hematocrit SRBC (2000r/min, 10min) 0.2ml, immunity is after 5 days, extract eyeball of mouse and get blood in centrifuge tube, place about 1 hour, solidification blood and tube wall are peeled off, centrifugal, collect serum, with physiological saline by serum doubling dilution, different dilution factor serum is placed in respectively in the solidifying brassboard of trace snow, every hole 100 μ l, add 0.5%SRBC suspension 100 μ l again, mixing, put in wet box, 37 DEG C of incubations 3 hours, record hemagglutination degree, calculating antibody product (serum two-fold dilution index and the aggegation degree sum of products).
Serum agglutination degree is divided into 5 grades, clicks standard determination.0 grade: SRBC all sinks, concentrate at the bottom of hole and form fine and close round point shape, surrounding liquid clear; I level: SRBC major part is deposited on bottom hole and becomes round point shape, and surrounding has the SRBC of a small amount of aggegation; II level: the SRBC of aggegation forms thin layer at the bottom of hole, a loose red point obviously can be seen in center; III level: the SRBC of aggegation all with paving be dispersed at the bottom of hole and become skim, may be seen indistinctly a small red dot at center; IV level: the SRBC of aggegation spreads uniformly to be dispersed at the bottom of hole and becomes skim, and grumeleuse becomes convolution shape sometimes.
The data obtained PEMS software carries out variance analysis, if tested material group Hemolysin product is higher than control group, and difference has conspicuousness, can judge that tested material is improved the effect of mice serum hemolysin level.
4.6 mouse carbonic clearance tests:
Animal gives sample after 30 days continuously, and the india ink that tail vein injection 1: 5 dilutes, treats that prepared Chinese ink injects, immediately timing.To inject after prepared Chinese ink 2,10 minutes, get blood 20 μ l from angular vein clump respectively, and be added in 2ml sodium carbonate liquor, with spectrophotometer at 600nm wavelength place test absorbance, do blank with sodium carbonate liquor.According to the weight of animals, liver weighs and spleen re-computation phagocytic index, and result is added up with variance analysis.If tested material group Hemolysin product is higher than control group, and difference has conspicuousness, can judge that tested material is improved the effect of the carbonic clearance ability of mouse monokaryon-macrophage.
4.7 Turnover of Mouse Peritoneal Macrophages engulf chicken red blood cell test (half intracorporal method):
Animal gives sample after 30 days continuously, every mouse lumbar injection 20% chicken erythrocyte suspension 1ml, 30 minutes, interval, cervical dislocation is put to death, and is fixed on mouse plate, cuts off abdominal skin, injecting normal saline 2ml, rotate mouse plate 1 minute, sucking-off abdominal cavity washing lotion 1ml, divide and drip in 2 sheets, 37 DEG C of incubators wet and incubate 30 minutes, use physiological saline rinsing, dry, fix with 1: 1 acetone methanol solution, 4%Giemsa-phosphate buffer dyes 3 minutes, dry with distilled water rinsing again, with oily mirror microscopy, calculate percentage phagocytosis and phagocytic index.Wherein, macrophage number × 100 of phagocytic rate (%)=the engulf macrophage number/counting of chicken red blood cell;
Phagocytic index=by engulf chicken red blood cell sum/counting macrophage number.
The phagocytic rate of acquired results is pressed
convert, in formula, P is phagocytic percentage, represents decimally, if the phagocytic rate of tested material or phagocytic index are apparently higher than control group, and difference has conspicuousness, can judge that tested material is improved the effect of macrophage phagocytic chicken red blood cell ability.
4.8 NK cells in mice determinations of activity:
Animal gives sample after 30 days continuously, and cervical dislocation is put to death, and gets spleen, makes splenocyte suspension (effector cell), get 24h YAC-1 cell after going down to posterity and add 1640 complete culture solutions, and adjustment cell concentration is 4 × 10
5individual/ml (target cell), get target cell and each 100 μ l effect target ratio (50: 1) of effector cell, add U-shaped 96 well culture plate, target cell Spontaneous release hole adds target cell and each 100 μ l of nutrient solution, maximum release aperture adds target cell and each 100 μ l of 1%NP40, above-mentionedly everyly be equipped with three multiple holes, with 37 DEG C, cultivate 4 hours in 5% CO2gas incubator, every hole Aspirate supernatant 100 μ l is placed in flat 96 well culture plates, add LDH matrix liquid 100 μ l simultaneously, reaction 3min, the HCL30 μ l that every hole adds 1mol/l stops, A value is measured at 490mm place with ELIASA.Calculate NK cytoactive as follows:
NK cytoactive %=(reacting hole A-Spontaneous release hole A)/(maximum release aperture A-Spontaneous release hole A) × 100;
NK cytoactive need be pressed
carry out data conversion, in formula, P is NK cytoactive, represents decimally, if the phagocytic rate of tested material or phagocytic index are apparently higher than control group, and difference has conspicuousness, can judge that tested material is improved the effect of NK cells in mice activity.
5 experimental results
5.1 experimental results judge
Strengthen immunocompetence function to judge: in cellular immune function, humoral immune function, monocytes/macrophages function, NK cytoactive four, in any two, result is positive, can judge that this tested material has develop immunitypty function.
Two experimental results wherein in cellular immune function assay project are the positive, or two of arbitrary experiment dosage group results are positive, can judge that cellular immune function assay result is as the positive.It is the positive that two experimental results in humoral immune function mensuration project are two dosage group results that are positive or arbitrary experiment, can judge that humoral immune function measurement result is positive.It is positive that two experimental results in monocytes/macrophages functional examination project are two dosage group results that are positive or arbitrary experiment, can judge that monocytes/macrophages functional examination result is positive.More than one dosage group result of NK cytoactive detection experiment is positive, can judge that NK cytoactive result is positive.
5.2 bee glue soft capsules on the impact of Mouse Weight in table 1.
Table 1 sample is on the impact of an immune treated animal body weight (gram)
Dosage different is as can be seen from Table 1 compared with control group, and the weight gain there was no significant difference of mouse, therefore the body weight of bee glue soft capsule to animal does not have a significant effect.
5.3 bee glue soft capsules are on the impact of mice organs body weight ratio: in table 2, table 3.
Table 2 bee glue soft capsule is on the impact of mouse spleen body weight ratio
Dosage different is as can be seen from Table 2 compared with control group, and the spleen weight ratio there was no significant difference of mouse, therefore the spleen weight ratio of bee glue soft capsule to animal does not have a significant effect.
Table 3 bee glue soft capsule is on the impact of mouse spleen body weight ratio
Dosage different is as can be seen from Table 3 compared with control group, and the thymus gland body weight ratio there was no significant difference of mouse, therefore the thymus gland body weight ratio of bee glue soft capsule on animal does not obviously affect.
5.4 bee glue soft capsules are on the impact of mouse cell immunologic function
A, bee glue soft capsule on the impact of mouse delayed allergy (DTH) in table 4.
Table 4 bee glue soft capsule is on the impact of mouse delayed allergy (DTH)
From table 4, the mouse swelling degree of the paw of water control group, solvent control group and 3 dosage groups affect no significant difference, think that the ability of bee glue soft capsule to mouse delayed allergy (DTH) does not have a significant effect.
B, bee glue soft capsule on the impact of the mouse spleen lymphocyte transformation experiment that ConA induces, in table 5
Table 5 bee glue soft capsule is on the impact of the mouse spleen lymphocyte transformation experiment that ConA induces
From table 5, water control group, solvent control group and 3 dosage groups, to there was no significant difference between the lymphopoiesis ability of mouse, think that bee glue soft capsule does not have a significant effect to raising mouse lymphocyte multiplication capacity.
5.4 bee glue soft capsules are on the impact of mouse humoral immune function
A, bee glue soft capsule on the impact of mouse antibodies cellulation number, in table 6.
Table 6 bee glue soft capsule is on the impact of mouse antibodies cellulation number
From table 6, water control group and low dose group, middle dosage group and the impact of high dose group on hemolysis plaque number have obvious difference, think that bee glue soft capsule has the ability increasing mouse antibodies cellulation number.
B, bee glue soft capsule on the impact of mice serum hemolysin level, in table 7.
Table 7 bee glue soft capsule is on the impact of mice serum hemolysin level
| Group | Number of animals (only) | Antibody product | P |
| Water control group | 10 | 143.3±23.8 | |
| Solvent control group | 10 | 149.9±32.5 | 0.611 |
| Low dose group | 10 | 152.0±26.6 | 0.451 |
| Middle dosage group | 10 | 157.5±24.3 | 0.204 |
| High dose group | 10 | 165.2±40.6 | 0.163 |
From table 7, the impact of water control group, solvent control group, low dose group and middle dosage group and high dose group antagonist product does not have obvious difference, thinks that bee glue soft capsule has no significant effect mice serum hemolysin level.
5.5 bee glue soft capsules are on the impact of mouse monokaryon-macrophage phagocytic function
A, bee glue soft capsule on the impact of mouse monokaryon-macrophage carbonic clearance ability, in table 8
Table 8 bee glue soft capsule is on the impact of mouse monokaryon-macrophage carbonic clearance ability
| Group | Number of animals (only) | Phagocytic index | P |
| Water control group | 10 | 4.78±1.44 | |
| Solvent control group | 10 | 4.57±1.03 | 0.715 |
| Low dose group | 10 | 4.96±0.86 | 0.730 |
| Middle dosage group | 10 | 5.03±1.17 | 0.678 |
| High dose group | 10 | 5.23±0.97 | 0.423 |
From table 8, water control group, solvent control group and 3 dosage groups, to there was no significant difference between the monocytes/macrophages carbonic clearance ability of mouse, think that bee glue soft capsule does not have a significant effect to mouse monokaryon-macrophage carbonic clearance ability.
B, bee glue soft capsule engulf the impact of chicken red blood cell ability, in table 9, table 10 to Turnover of Mouse Peritoneal Macrophages.
Table 9 bee glue soft capsule is on the impact of Turnover of Mouse Peritoneal Macrophages chicken red blood cell phagocytic rate
Table 10 bee glue soft capsule is on the impact of Turnover of Mouse Peritoneal Macrophages chicken red blood cell phagocytic index
| Group | Number of animals (only) | Phagocytic index | P |
| Water control group | 10 | 0.43±0.32 | |
| Solvent control group | 10 | 0.49±0.33 | 0.697 |
| Low dose group | 10 | 0.80±0.76 | 0.185 |
| Middle dosage group | 10 | 1.14±0.63 | 0.007 |
| High dose group | 10 | 2.04±1.22 | 0.002 |
From table 9 and table 10, water control group, middle dosage group and high dose group are engulfed between chicken red blood cell ability Turnover of Mouse Peritoneal Macrophages and are had significant difference, and thinking that bee glue soft capsule has increases the ability that Turnover of Mouse Peritoneal Macrophages engulfs chicken red blood cell.
5.6 bee glue soft capsules on the impact of NK cells in mice activity, in table 11.
Table 11 bee glue soft capsule is on the impact of NK cells in mice activity
From table 11, water control group and high dose group have significant difference between NK cells in mice activity, think that bee glue soft capsule can strengthen NK cells in mice activity.
To sum up, per os gives the bee glue soft capsule 30 days of mouse various dose group, Mouse Weight is increased and has no adverse effects, on mouse spleen and thymus gland body weight ratio without impact, the humoral immune function of mouse, monocytes/macrophages phagocytic activity, NK cytoactive detection result are the positive, cellular immune function assay result is negative, shows that bee glue soft capsule has the effect of develop immunitypty function.
Embodiment 2
A kind of bee glue soft capsule, the content comprising utricule and enclose in utricule, content is made up of the raw material of following component and weight percent content: propolis 18, ginkgo biloba p.e 1, PEG400 61, glycerine 20; Wherein, the general flavone content in propolis is 20wt%, containing 24wt% general flavone in ginkgo biloba p.e.
A preparation method for bee glue soft capsule, the method comprises the following steps:
(1) make utricule: with gelatin, glycerine and pure water for utricule raw material, glycerine and pure water are injected in glue pot, is heated with stirring to 70 DEG C, add gelatin again, continue stirring final vacuum deaeration in 20 minutes, make and obtain utricule, wherein the weight ratio of gelatin, glycerine and pure water is 20: 1: 5;
(2) prepare content: after first propolis being pulverized and PEG400 and glycerine at 60 DEG C, fully mix 35min, and then add ginkgo biloba p.e and fully mix 50min, mistake colloid mill 2 times, obtains the content mixed;
(3) prepare capsule: the content that step (2) is prepared and step (1) make obtain utricule through pelleting, shape, wash ball, drying, pick ball and packing step, prepare bee glue soft capsule, every capsules is 500 milligrams containing content.
Embodiment 3
A kind of bee glue soft capsule, the content comprising utricule and enclose in utricule, content is made up of the raw material of following component and weight percent content: propolis 20, ginkgo biloba p.e 20, PEG400 50, glycerine 10; Wherein, the general flavone content in propolis is 32wt%, containing 24wt% general flavone in ginkgo biloba p.e.
A preparation method for bee glue soft capsule, the method comprises the following steps:
(1) make utricule: with gelatin, glycerine and pure water for utricule raw material, glycerine and pure water are injected in glue pot, is heated with stirring to 60 DEG C, add gelatin again, continue stirring final vacuum deaeration in 30 minutes, make and obtain utricule, wherein the weight ratio of gelatin, glycerine and pure water is 5: 1: 5;
(2) prepare content: after first propolis being pulverized and PEG400 and glycerine at 60 DEG C, fully mix 30min, and then add ginkgo biloba p.e and fully mix 40min, mistake colloid mill 2 times, obtains the content mixed;
(3) prepare capsule: the content that step (2) is prepared and step (1) make obtain utricule through pelleting, shape, wash ball, drying, pick ball and packing step, prepare bee glue soft capsule, every capsules is 500 milligrams containing content, and every capsules is 500 milligrams containing content.
Embodiment 4
A kind of bee glue soft capsule, the content comprising utricule and enclose in utricule, content is made up of the raw material of following component and weight percent content: propolis 18, ginkgo biloba p.e 2, PEG400 70, glycerine 10; Wherein, the general flavone content >=18wt% in propolis, containing 24wt% general flavone in ginkgo biloba p.e.
A preparation method for bee glue soft capsule, the method comprises the following steps:
(1) make utricule: with gelatin, glycerine and pure water for utricule raw material, glycerine and pure water are injected in glue pot, is heated with stirring to 70 DEG C, add gelatin again, continue stirring final vacuum deaeration in 20 minutes, make and obtain utricule, wherein the weight ratio of gelatin, glycerine and pure water is 20: 10: 20;
(2) prepare content: after first propolis being pulverized and PEG400 and glycerine at 70 DEG C, fully mix 20min, and then add ginkgo biloba p.e and fully mix 60min, mistake colloid mill 3 times, obtains the content mixed;
(3) prepare capsule: the content that step (2) is prepared and step (1) make obtain utricule through pelleting, shape, wash ball, drying, pick ball and packing step, prepare bee glue soft capsule, every capsules is 500 milligrams containing content.
Embodiment 5
A kind of bee glue soft capsule, the content comprising utricule and enclose in utricule, content is made up of the raw material of following component and weight percent content: propolis 30, ginkgo biloba p.e 10, PEG400 50, glycerine 10; General flavone content >=18wt% in propolis, containing 24wt% general flavone in ginkgo biloba p.e.
A preparation method for bee glue soft capsule, the method comprises the following steps:
(1) make utricule: with gelatin, glycerine and pure water for utricule raw material, glycerine and pure water are injected in glue pot, is heated with stirring to 65 DEG C, add gelatin again, continue stirring final vacuum deaeration in 25 minutes, make and obtain utricule, wherein the weight ratio of gelatin, glycerine and pure water is 10: 5: 5;
(2) prepare content: after first propolis being pulverized and PEG400 and glycerine at 50 DEG C, fully mix 40min, and then add ginkgo biloba p.e and fully mix 30min, mistake colloid mill 1 time, obtains the content mixed;
(3) prepare capsule: the content that step (2) is prepared and step (1) make obtain utricule through pelleting, shape, wash ball, drying, pick ball and packing step, prepare bee glue soft capsule, every capsules is 500 milligrams containing content.
Claims (4)
1. a bee glue soft capsule, the content comprising utricule and enclose in utricule, it is characterized in that, described content is made up of the raw material of following component and weight percent content:
General flavone content >=18wt% in described propolis,
Containing 24wt% general flavone in described ginkgo biloba p.e.
2. a kind of bee glue soft capsule according to claim 1, is characterized in that, described content is made up of the raw material of following component and weight percent content:
3. a preparation method for bee glue soft capsule as claimed in claim 1 or 2, is characterized in that, the method comprises the following steps:
(1) make utricule: with gelatin, glycerine and pure water for utricule raw material, glycerine and pure water are injected in glue pot, is heated with stirring to 60 ~ 70, DEG C add gelatin again, continue to stir final vacuum deaeration in 20-30 minute, make and obtain utricule;
(2) prepare content: after first propolis being pulverized and PEG400 and glycerine at 50 ~ 70 DEG C, fully mix 20 ~ 40min, and then add ginkgo biloba p.e and fully mix 30 ~ 60min, cross colloid mill 1 ~ 3 time, obtain the content mixed;
(3) prepare capsule: the content that step (2) is prepared and step (1) make obtain utricule through pelleting, shape, wash ball, drying, pick ball and packing step, prepare bee glue soft capsule.
4. the preparation method of a kind of bee glue soft capsule according to claim 3, is characterized in that, in step (1), the weight ratio of gelatin, glycerine and pure water is 5 ~ 20 ︰ 1 ~ 10 ︰ 5 ~ 20.
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| CN103520224A (en) * | 2013-10-23 | 2014-01-22 | 扈建民 | Traditional Chinese medicinal combination containing propolis and application thereof |
| CN103815395A (en) * | 2013-12-25 | 2014-05-28 | 伊犁百信草原蜂业有限责任公司 | Propolis-safflower oil soft capsule and preparation method thereof |
| CN104585574A (en) * | 2015-01-07 | 2015-05-06 | 北京蜜蜂堂生物医药股份有限公司 | Propolis soft capsule product and preparation method thereof |
| CN105079044A (en) * | 2015-09-23 | 2015-11-25 | 李�杰 | Preparation method of soft capsules containing Chinese caterpillar funguses |
| CN105962356A (en) * | 2016-05-03 | 2016-09-28 | 宣城柏维力生物工程有限公司 | Health-care propolis soft capsules and preparation method thereof |
| CN106361790A (en) * | 2016-08-26 | 2017-02-01 | 湖北新阳蜂业有限公司 | Bee propolis capsule and preparation method thereof |
| CN106491653A (en) * | 2016-11-02 | 2017-03-15 | 深圳市荣格保健品有限公司 | A kind of bee glue soft capsule and preparation method thereof |
| CN106722612A (en) * | 2016-12-02 | 2017-05-31 | 芜湖市诺康生物科技有限公司 | A kind of bee glue soft capsule and preparation method thereof |
| CN108272832A (en) * | 2018-04-10 | 2018-07-13 | 辽宁仙草堂药业有限公司 | A kind of ginkgo leaf soft capsule and preparation method thereof |
| CA3130015A1 (en) * | 2019-02-19 | 2020-08-27 | ApiotiX Technologies d.o.o. | Liquid propolis extract, its formulation and use thereof |
| CN113599399A (en) * | 2021-09-02 | 2021-11-05 | 威海金格生物科技有限公司 | Pure biological preparation for enhancing immunity and improving glycolipid metabolism and application thereof |
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| CN100340250C (en) * | 2005-06-09 | 2007-10-03 | 周云川 | Composite propolis soft capsule |
| CN101637491B (en) * | 2009-06-10 | 2011-03-30 | 北京一品堂医药科技有限公司 | Health-care food having functions of assisting antidiabetics and assisting antiatheroscloresis, and preparation method thereof |
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