CN103190621A - Propolis soft capsule and preparation method thereof - Google Patents
Propolis soft capsule and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a propolis soft capsule and a preparation method thereof. The preparation method comprises the following steps: injecting glycerin and pure water into an aerosol can, stirring and heating to 60-70 DEG C, adding gelatin, continuing to stir for 20-30min, performing vacuum defoaming to prepare a capsule body; firstly, grinding propolis and then fully mixing polyethylene glycol and glycerol for 20-40min at 50-70 DEG C, then adding folium ginkgo extract, fully mixing for 30-60min, performing milling for one to three times by a colloid mill to obtain evenly mixed content; and performing pelleting, shaping, pellet washing, drying, pellet sorting and subpackaging to the content and the capsule body, to prepare the propolis soft capsule. Compared with the prior art, the soft capsule has the advantages of being good in bioavailability, excellent in drug stability, capable of covering unpleasant odor, accurate in dosage, sealed, safe, convenient to carry and use, and the like, and the propolis soft capsule has the function of increasing the immunity function.
Description
Technical field
The present invention relates to the health food technology field, especially relate to a kind of bee glue soft capsule and preparation method thereof.
Background technology
Immunity refers to a series of intrinsic protective system that body is set up for a long time, and strengthening immunity is very important to guaranteeing healthy, and the reduction of body immunity can cause various diseases.Immunity has three kinds of functions to body: 1, defense function; 2, Selfstabilizing function; Function is inspected in 3 immunity.With the balance of keeping vivo environment and stable, immunologic dysfunction then immunity disease can occur by these functions, even tumour takes place.
At present, through existing thousands of of the health food of the enhancing immunity of the Ministry of Public Health and State Food and Drug Administration approval, have the function factor that improves immunity function polysaccharide, flavonoids, saponins, protein etc. are arranged.
What propolis contained enriches and the unique biological active material, make its have antibiotic, anti-inflammatory, antipruritic, anti-oxidant, strengthen immune, hypoglycemic, reducing blood lipid, multiple function such as antitumor, human body there are medical treatment, health-care effect widely, now become the focus of various countries' scientific research, and become emerging health products and extremely praise highly.
Summary of the invention
Purpose of the present invention is exactly to provide in order to overcome the defective that above-mentioned prior art exists that a kind of bioavilability is good, medicine stability good, have bee glue soft capsule that strengthens immunity function and preparation method thereof.
Purpose of the present invention can be achieved through the following technical solutions:
A kind of bee glue soft capsule comprises the content in utricule and the inclosure utricule, and described content is made by the raw material of following component and weight percent content:
Described content is made by the raw material of following component and weight percent content:
General flavone content 〉=18wt% in the described propolis.
Described ginkgo biloba p.e contains the general flavone of 24wt%.
A kind of preparation method of bee glue soft capsule, this method may further comprise the steps:
(1) makes utricule: be the utricule raw material with gelatin, glycerine and pure water, glycerine and pure water are injected in the glue pot, be heated with stirring to 60～70 ℃, add gelatin again, continue to stir final vacuum deaeration in 20-30 minute, make obtaining utricule;
(2) preparation content: at first propolis is pulverized back and PEG400 and glycerine and is fully mixed 20～40min down at 50～70 ℃, and then add ginkgo biloba p.e and fully mix 30～60min, colloid mill 1～3 time, the content that obtains mixing;
(3) preparation capsule: content and step (1) making of step (2) preparation are obtained utricule process pelleting, finalize the design, wash ball, drying, choose ball and packing step, prepare bee glue soft capsule.
The weight ratio of gelatin, glycerine and pure water is 5～20: 1～10 in the step (1): 5～20.
The propolis that the present invention adopts, the main active of ginkgo biloba p.e all are flavonoids, have the health-care effect that strengthens immunity.
The propolis composition is quite complicated, main component is flavone compound, also have multiple compounds such as phenols, alcohols, acids lipid, also contain trace element such as a spot of iron, calcium, copper, silicon, manganese, lead, tin and vitamin A, Cobastab, several amino acids, enzyme, forulic acid etc. in addition.Propolis or propolis extract are pharmaceutically as anaphylactogen, radioresistance, antitumor, immune response stimulating etc.
The immunological enhancement of propolis is inseparable with the physical behavior of its complicated chemical composition and its extract.VA in the propolis is a kind of centrally acting adjuvant, and immune maincenter is worked, and can stimulate the enhancing of antibody response, and VB can strengthen humoral immunity, strengthens the antibody amount.Zn in the propolis can excite the immunity of animal.Immunocyte is in the DNA building-up process, several dependence metalloenzyme are being controlled the production breeding of cell, lack the lymphocytic immunity function of the T of Zn and have very big infringement, to cause the phagocyte significant change, thereby lose its polymorphism and pseudopodium and become level and smooth round cell immunity function is descended.A large amount of experiments prove that also the many enzymes in the propolis, alcohols, lipid, acid have effect widely to the immune system of body.Pinocembrin in the propolis, Galangin, kaempferia galamga element, tonka-bean benzoate, caffeic acid fat and propolis extract there is antibacterial activity, itself does not have antigenicity, but can play immunoadjuvant function, the generation of energy enhancing antibody, the content of total serum protein and gamma globulin is increased, the phagocytic activity of leucocyte and macrophage strengthens, and the specificity of body and non-specific immunity strengthen, and the immunological enhancement of propolis is the synergistic result of above all materials.
Ginkgo biloba p.e comprises ginkgolides and flavonoids (based on Quercetin, kaempferia galamga rope, bigcatkin willow syphilis), except having effects such as expansion cardiovascular and cerebrovascular, diastole bronchus, antibiotic, anti-inflammatory, immune function of human body is also had humidification.
Propolis can be partly dissolved in PEG400, is decentralized medium with the PEG400, and it is even to be conducive to propolis and ginkgo biloba p.e suspendible in medium, is conducive to raw material and disperses in alimentary canal, is easy to absorb.
Compared with prior art, soft capsule of the present invention has that bioavilability is good, medicine stability is good, cover bad smell, dosage accurately, sealing, safely, carry and advantage such as easy to use, bee glue soft capsule of the present invention has the effect that strengthens immunity function.
The specific embodiment
The present invention is described in detail below in conjunction with specific embodiment.
Embodiment 1
A kind of bee glue soft capsule comprises the content in utricule and the inclosure utricule, and content is made by the raw material of following component and weight percent content: propolis 50, ginkgo biloba p.e 5, PEG400 40, glycerine 5; Wherein, the general flavone content 〉=18wt% in the propolis, ginkgo biloba p.e contains the 24wt% general flavone.
A kind of preparation method of bee glue soft capsule, this method may further comprise the steps:
(1) makes utricule: be the utricule raw material with gelatin, glycerine and pure water, glycerine and pure water are injected in the glue pot, be heated with stirring to 60 ℃, add gelatin again, continue to stir final vacuum deaeration in 30 minutes, make and obtain utricule, wherein the weight ratio of gelatin, glycerine and pure water is 5: 10: 20;
(2) preparation content: at first propolis is pulverized back and PEG400 and glycerine and is fully mixed 20～40min down at 50～70 ℃, and then add ginkgo biloba p.e and fully mix 30～60min, colloid mill 1～3 time, the content that obtains mixing;
(3) preparation capsule: content and step (1) making of step (2) preparation are obtained utricule process pelleting, finalize the design, wash ball, drying, choose ball and packing step, prepare bee glue soft capsule, it is 500 milligrams that every capsules contains content.
Enhancing immunity function to the bee glue soft capsule of present embodiment preparation detects.
1, sample: the bee glue soft capsule of present embodiment preparation
2, experimental animal and environment: SPF Kunming kind small white mouse, credit number: SCXK (Shanghai) 2002-0010, body weight 18～22 grams, female 100, male 100, be divided into four immunity and organize greatly, 50 of every big groups, the mouse of the big group of each immunity is divided into water control group, solvent control group, basic, normal, high dosage group, each 10.Immunity one group (50 of female mices) is used for delayed allergy (DTH) experiment, the mensuration of serum hemolysin, the mensuration of antibody-producting cell number; Immunity two groups (50 of male mices) is used for mouse carbon and cleans up experiment; Immunity three groups (50 of female mices) is used for Turnover of Mouse Peritoneal Macrophages and engulfs the chicken red blood cell experiment; Immunity four groups (50 of male mices) is used for mouse lymphocyte transformation experiment and NK cells in mice activity experiment mensuration.22 ± 2 ℃ of experimental situation temperature, relative humidity 50% ± 10%.
3, dosage designs and tried thing and gives mode: bee glue soft capsule of the present invention is 1.0g/60kg body weight every day to the RD of human body, be equivalent to 0.0167g/kg.BW, determine the low middle Senior Three kind dosage of mouse by 5,10,30 multiple doses that are equivalent to the human body RD, dosage to mouse in the present embodiment is made as 0.083g/kg.BW respectively, 0.167g/kg.BW, 0.50g/kg.BW three kinds, per os is irritated stomach once a day and is given mouse and tried thing, irritates the long-pending 10ml/kg.BW that presses of body of stomach.Tried thing with the corn oil preparation before irritating stomach, the concentration of being tried thing in the basic, normal, high dosage group is respectively 8.33g/L, 16.667g/L and 50.00g/L, negative control group replaces being tried thing with isopyknic distilled water, the solvent control group replaces being tried thing with isopyknic corn oil, the every immune indexes of test after 30 days.Mouse feeds with outbreeding system mouse special feed and raises.
4, experimental technique
4.1 organ weight ratio pH-value determination pH
The cervical vertebra dislocation was put to death after mouse was weighed, and got its thymus gland, spleen, removed most manadesma, blotted the organ surface blood stains and weighed calculating thymus gland body weight ratio and spleen body weight ratio with filter paper.The gained data are carried out variance analysis with the PEMS statistical software.
4.2 delayed allergy (the sufficient sole of the foot thickens method) (DTH)
Get sheep blood, with physiological saline washing three times, every mouse is through lumbar injection 2% (v/v, dispose with physiological saline) hematocrit SRBC (2000r/min, 10min) 0.2ml, after the sensitization 4 days, sufficient sole of the foot thickness about measurement, same position is measured 3 times, average, then at measuring point hypodermic injection 20% (v/v disposes with physiological saline) hematocrit SRBC20 μ l, inject sufficient sole of the foot thickness about measurement in back 24 hours, represent the degree of DTH with the difference of sufficient sole of the foot thickness before and after attacking.The gained data are measurement data, carry out variance analysis with the SPSS statistical software, if tried thing attack before and after the difference of sufficient sole of the foot thickness be higher than control group, and difference has conspicuousness (P＜0.05), can conclude that this is tried thing the effect that improves mouse delayed allergy ability is arranged.
4.3ConA the mouse spleen lymphocyte conversion test (mtt assay) of inducing
Animal gives sample after 30 days continuously, the cervical vertebra dislocation is put to death, get spleen, make splenocyte suspension, adjusting cell concentration is 3 * 106/ml, cell suspension is divided into two holes adds 24 well culture plate China, every hole 1ml, one hole adds 75 μ lConA liquid (being equivalent to 7.5 μ g/ml), and 5%CO is put in contrast in another hole
2, cultivate 68h for 37 ℃.Cultivate and finish preceding 4h, every hole gentle aspiration supernatant 0.7ml, adding 0.7ml does not contain the PRMI1640 nutrient solution of calf serum, add MTT (5mg/ml) 50 μ l/ holes simultaneously, continue to cultivate 4h, after cultivating end, every hole adds 1ml acid isopropyl alcohol, piping and druming mixing, purple crystal is dissolved fully after, liquid is moved in 96 orifice plates then, every hole adds 3 parallel samples, measure absorbance (A value) with ELIASA at the 570nm wavelength, calculate the poor of test hole A value and control wells A value, to represent lymphocytic competence for added value.The result analyzes with variance analysis.If tried the lymphocytic multiplication capacity of thing group and be higher than control group, and difference has conspicuousness, can judge that this is tried thing the effect that improves the mouse spleen lymphocyte conversion capability that ConA induces is arranged.
4.4 antibody-producting cell is measured (Jerne improves slide method)
Animal gives sample after 30 days continuously, and after 5 days, the dislocation of animal cervical vertebra is put to death, and takes out spleen, makes splenocyte suspension with defiber Mianyang erythrocyte immune, and adjusting cell concentration is 5 * 10
6Individual/ml.After top layer culture medium (the 1g agarose adds distilled water 100ml) heating for dissolving, put into 45 ℃ of water bath heat preservations, mix packing small test tube, every pipe 0.5ml with the Hanks liquid of equivalent pH7.2-7.4,2 times of concentration, in pipe, add 50 μ l10%SRBC again, 20 μ l splenocyte suspensions, mixing is poured on the 6cm plate of brushing thin agar layer rapidly, after continuing incubation 1.5h, counting hemolysis plaque number.The result adds up with variance analysis.If tried the lymphocytic multiplication capacity of thing group and be higher than control group, and difference has conspicuousness, can judge that this is tried thing the effect that strengthens the mouse antibodies cell number is arranged.
4.5 mice serum hemolysin test:
Get sheep blood, with physiological saline washing 3 times, every mouse is through lumbar injection 2% (with physiological saline configuration, volume ratio) hematocrit SRBC (2000r/min, 10min) 0.2ml, after the immunity 5 days, extract eyeball of mouse and get blood in centrifuge tube, placed about 1 hour, solidification blood and tube wall are peeled off, centrifugal, collect serum, with physiological saline with the serum doubling dilution, place trace snow to coagulate in the brassboard respectively different dilution factor serum, every hole 100 μ l add 0.5%SRBC suspension 100 μ l, mixing again, put in the wet box, 37 ℃ of incubations 3 hours, record hemagglutination degree, calculating antibody product (serum two-fold dilution index and the aggegation degree sum of products).
Serum aggegation degree is divided into 5 grades, clicks standard determination.0 grade: SRBC all sinks, and concentrates on and forms fine and close round point shape, liquid clear all around at the bottom of the hole; The I level: the SRBC major part is deposited on the bottom, hole and becomes round point shape, and the SRBC of a small amount of aggegation is arranged all around; The II level: the SRBC of aggegation forms thin layer at the bottom of the hole, and a loose red point can be obviously seen at the center; The III level: the SRBC of aggegation all with the shop become skim at the bottom of being dispersed in the hole, a small red dot mays be seen indistinctly at the center; The IV level: the SRBC of aggegation shop uniformly becomes skim at the bottom of being dispersed in the hole, and grumeleuse becomes the convolution shape sometimes.
The gained data are carried out variance analysis with PEMS software, if tried thing group serum hemolysin antibody product and be higher than control group, and difference has conspicuousness, can judge that being tried thing has the effect that improves the mice serum hemolysin level.
4.6 mouse carbon clearance test:
Animal gives sample after 30 days continuously, and the india ink of tail vein injection dilution in 1: 5 treats that prepared Chinese ink injects timing immediately.Injected behind the prepared Chinese ink 2,10 minutes, and got blood 20 μ l from the angular vein clump respectively, and it is added in the 2ml sodium carbonate liquor, at 600nm wavelength place test absorbance, do blank with sodium carbonate liquor with spectrophotometer.According to the weight of animals, the heavy and spleen re-computation phagocytic index of liver, the result adds up with variance analysis.If tried thing group serum hemolysin antibody product and be higher than control group, and difference has conspicuousness, can judge the effect that the carbon that tried thing and have raising mouse monokaryon-macrophage is cleaned up ability.
4.7 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell test (half intracorporal method):
Animal gives sample after 30 days continuously, every mouse lumbar injection 20% chicken erythrocyte suspension 1ml, 30 minutes at interval, the cervical vertebra dislocation is put to death, and is fixed on the mouse plate, cuts off abdominal skin, injecting normal saline 2ml, rotated the mouse plate 1 minute, sucking-off abdominal cavity washing lotion 1ml divides and drips on 2 sheet glass sheets, 37 ℃ of wet incubating 30 minutes of incubator, use the physiological saline rinsing, dry, fix with 1: 1 acetone methanol solution, 4%Giemsa-phosphate buffer dyeing 3 minutes, dry with the distilled water rinsing again, with oily mirror microscopy, calculate percentage phagocytosis and phagocytic index.Wherein, phagocytic rate (%)=engulf macrophage number * 100 of the macrophage number/counting of chicken red blood cell;
The macrophage number of chicken red blood cell sum/counting that phagocytic index=quilt is engulfed.
Gained result's phagocytic rate is pressed
Convert, P is phagocytic percentage in the formula, expression decimally, if tried the phagocytic rate of thing or phagocytic index apparently higher than control group, and difference has conspicuousness, can judge that being tried thing has the effect that improves macrophage phagocytic chicken red blood cell ability.
4.8 NK cells in mice determination of activity:
Animal gives sample after 30 days continuously, and the cervical vertebra dislocation is put to death, and gets spleen, makes splenocyte suspension (effector cell), gets the back 24h YAC-1 cell that goes down to posterity and adds 1640 complete culture solutions, and adjusting cell concentration is 4 * 10
5Individual/ml (target cell), get each 100 μ l of target cell and effector cell and imitate target than (50: 1), add U-shaped 96 well culture plates, target cell nature release aperture adds target cell and each 100 μ l of nutrient solution, maximum release aperture adds target cell and each 100 μ l of 1%NP40, above-mentioned every three multiple holes that are equipped with, with cultivated 4 hours in 37 ℃, 5% CO2gas incubator, every hole is drawn supernatant 100 μ l and is placed flat 96 well culture plates, add LDH matrix liquid 100 μ l simultaneously, reaction 3min, the HCL30 μ l that every hole adds 1mol/l stops, and measures the A value at the 490mm place with ELIASA.Calculate the NK cytoactive as follows:
NK cytoactive %=(reacting hole A-nature release aperture A)/(maximum release aperture A-nature release aperture A) * 100;
The NK cytoactive need be pressed
Carry out data transaction, P is the NK cytoactive in the formula, expression decimally, if tried the phagocytic rate of thing or phagocytic index apparently higher than control group, and difference has conspicuousness, can judge that being tried thing has the effect that improves the NK cells in mice activity.
5 experimental results
5.1 experimental result is judged
Strengthening the immunocompetence function judges: any two aspects positive as a result aspect four of cellular immune functions, humoral immune function, monokaryon-macrophage function, NK cytoactive, can judge that this is tried thing and has the effect of the immunity function of enhancing.
Wherein two experimental results in the cellular immune function assay project are all positive, or two dosage group results of arbitrary experiment are positive, can judge that the cellular immune function assay result is positive.Two experimental results in the humoral immune function mensuration project all two dosage group results positive or arbitrary experiment are positive, can judge the humoral immune function measurement result positive.Two experimental results in monokaryon-macrophage function mensuration project are two dosage groups positive as a result of positive or arbitrary experiment all, can judge monokaryon-macrophage function measurement result positive.An above dosage group of NK cytoactive determination experiment is the positive as a result, can judge the NK cytoactive positive as a result.
5.2 bee glue soft capsule sees Table 1 to the influence of mouse body weight.
Table 1 sample is to the influence of an immune treated animal body weight (gram)
Different as can be seen from Table 1 dosage are compared with control group, the weight gain there was no significant difference of mouse, so bee glue soft capsule is to the not obviously influence of body weight of animal.
5.3 bee glue soft capsule is to the influence of mouse organ weight ratio value: see Table 2, table 3.
Table 2 bee glue soft capsule is to the influence of mouse spleen body weight ratio
Different as can be seen from Table 2 dosage are compared with control group, the spleen body weight ratio there was no significant difference of mouse, so bee glue soft capsule is to the not obviously influence of spleen body weight ratio of animal.
Table 3 bee glue soft capsule is to the influence of mouse spleen body weight ratio
Different as can be seen from Table 3 dosage are compared with control group, the thymus gland body weight ratio there was no significant difference of mouse, so bee glue soft capsule is to the not obviously influence of thymus gland body weight ratio of animal.
5.4 bee glue soft capsule is to the mouse cell Immune Effects
A, bee glue soft capsule see Table 4 to the influence of mouse delayed allergy (DTH).
Table 4 bee glue soft capsule is to the influence of mouse delayed allergy (DTH)
By table 4 as seen, the mouse swelling degree of the paw of water control group, solvent control group and 3 dosage groups influence no significant difference, think the ability not obviously influence of bee glue soft capsule to mouse delayed allergy (DTH).
The influence of the mouse spleen lymphocyte transformation experiment that B, bee glue soft capsule induce ConA sees Table 5
The influence of the mouse spleen lymphocyte transformation experiment that table 5 bee glue soft capsule is induced ConA
By table 5 as seen, water control group, solvent control group and 3 dosage groups think that to there was no significant difference between the lymphopoiesis ability of mouse bee glue soft capsule is to improving the not obviously influence of mouse lymphocyte multiplication capacity.
5.4 bee glue soft capsule is to the influence of mouse humoral immune function
A, bee glue soft capsule see Table 6 to the influence of mouse antibodies cellulation number.
Table 6 bee glue soft capsule is to the influence of mouse antibodies cellulation number
By table 6 as seen, water control group and low dose group, middle dosage group and high dose group have evident difference to the influence of hemolysis plaque number, think that bee glue soft capsule has the ability of increase mouse antibodies cellulation number.
B, bee glue soft capsule see Table 7 to the influence of mice serum hemolysin level.
Table 7 bee glue soft capsule is to the influence of mice serum hemolysin level
| Group | Number of animals (only) | The antibody product | P |
| The water control group | 10 | 143.3±23.8 | ? |
| The solvent control group | 10 | 149.9±32.5 | 0.611 |
| Low dose group | 10 | 152.0±26.6 | 0.451 |
| Middle dosage group | 10 | 157.5±24.3 | 0.204 |
| High dose group | 10 | 165.2±40.6 | 0.163 |
By table 7 as seen, the influence of water control group, solvent control group, low dose group and middle dosage group and high dose group antagonist product does not have evident difference, thinks that bee glue soft capsule has no significant effect the mice serum hemolysin level.
5.5 bee glue soft capsule is to the influence of mouse monokaryon-macrophage phagocytic function
A, bee glue soft capsule are cleaned up the influence of ability, are seen Table 8 mouse monokaryon-macrophage carbon
Table 8 bee glue soft capsule is cleaned up the influence of ability to mouse monokaryon-macrophage carbon
| Group | Number of animals (only) | Phagocytic index | P |
| The water control group | 10 | 4.78±1.44 | ? |
| The solvent control group | 10 | 4.57±1.03 | 0.715 |
| Low dose group | 10 | 4.96±0.86 | 0.730 |
| Middle dosage group | 10 | 5.03±1.17 | 0.678 |
| High dose group | 10 | 5.23±0.97 | 0.423 |
By table 8 as seen, water control group, solvent control group and 3 dosage groups are cleaned up there was no significant difference between the ability to monokaryon-macrophage carbon of mouse, think that bee glue soft capsule cleans up not obviously influence of ability to mouse monokaryon-macrophage carbon.
B, bee glue soft capsule engulf the influence of chicken red blood cell ability to Turnover of Mouse Peritoneal Macrophages, see Table 9, table 10.
Table 9 bee glue soft capsule is to the influence of Turnover of Mouse Peritoneal Macrophages chicken red blood cell phagocytic rate
Table 10 bee glue soft capsule is to the influence of Turnover of Mouse Peritoneal Macrophages chicken red blood cell phagocytic index
| Group | Number of animals (only) | Phagocytic index | P |
| The water control group | 10 | 0.43±0.32 | ? |
| The solvent control group | 10 | 0.49±0.33 | 0.697 |
| Low dose group | 10 | 0.80±0.76 | 0.185 |
| Middle dosage group | 10 | 1.14±0.63 | 0.007 |
| High dose group | 10 | 2.04±1.22 | 0.002 |
By table 9 and table 10 as seen, water control group, middle dosage group and high dose group are engulfed between the chicken red blood cell ability Turnover of Mouse Peritoneal Macrophages and are had significant difference, think that bee glue soft capsule has the ability that Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell that increases.
5.6 bee glue soft capsule sees Table 11 to the influence of NK cells in mice activity.
Table 11 bee glue soft capsule is to the influence of NK cells in mice activity
By table 11 as seen, water control group and high dose group think that to having significant difference between the NK cells in mice activity bee glue soft capsule can strengthen the NK cells in mice activity.
To sum up, per os gives the bee glue soft capsule 30 days of mouse various dose group, weight of mice is had no adverse effects, mouse spleen and thymus gland body weight ratio there is not influence, the humoral immune function of mouse, monokaryon-macrophage phagocytic ability, NK cytoactive measurement result are all positive, cellular immune function assay is feminine gender as a result, shows that bee glue soft capsule has the effect that strengthens immunity function.
Embodiment 2
A kind of bee glue soft capsule comprises the content in utricule and the inclosure utricule, and content is made by the raw material of following component and weight percent content: propolis 18, ginkgo biloba p.e 1, PEG400 61, glycerine 20; Wherein, the general flavone content in the propolis is 20wt%, and ginkgo biloba p.e contains the 24wt% general flavone.
A kind of preparation method of bee glue soft capsule, this method may further comprise the steps:
(1) makes utricule: be the utricule raw material with gelatin, glycerine and pure water, glycerine and pure water are injected in the glue pot, be heated with stirring to 70 ℃, add gelatin again, continue to stir final vacuum deaeration in 20 minutes, make and obtain utricule, wherein the weight ratio of gelatin, glycerine and pure water is 20: 1: 5;
(2) preparation content: at first propolis is pulverized back and PEG400 and glycerine and is fully mixed 35min down at 60 ℃, and then add ginkgo biloba p.e and fully mix 50min, colloid mill 2 times, the content that obtains mixing;
(3) preparation capsule: content and step (1) making of step (2) preparation are obtained utricule process pelleting, finalize the design, wash ball, drying, choose ball and packing step, prepare bee glue soft capsule, it is 500 milligrams that every capsules contains content.
Embodiment 3
A kind of bee glue soft capsule comprises the content in utricule and the inclosure utricule, and content is made by the raw material of following component and weight percent content: propolis 20, ginkgo biloba p.e 20, PEG400 50, glycerine 10; Wherein, the general flavone content in the propolis is 32wt%, and ginkgo biloba p.e contains the 24wt% general flavone.
A kind of preparation method of bee glue soft capsule, this method may further comprise the steps:
(1) makes utricule: be the utricule raw material with gelatin, glycerine and pure water, glycerine and pure water are injected in the glue pot, be heated with stirring to 60 ℃, add gelatin again, continue to stir final vacuum deaeration in 30 minutes, make and obtain utricule, wherein the weight ratio of gelatin, glycerine and pure water is 5: 1: 5;
(2) preparation content: at first propolis is pulverized back and PEG400 and glycerine and is fully mixed 30min down at 60 ℃, and then add ginkgo biloba p.e and fully mix 40min, colloid mill 2 times, the content that obtains mixing;
(3) preparation capsule: content and step (1) making of step (2) preparation are obtained utricule process pelleting, finalize the design, wash ball, drying, choose ball and packing step, prepare bee glue soft capsule, it is 500 milligrams that every capsules contains content, and it is 500 milligrams that every capsules contains content.
Embodiment 4
A kind of bee glue soft capsule comprises the content in utricule and the inclosure utricule, and content is made by the raw material of following component and weight percent content: propolis 18, ginkgo biloba p.e 2, PEG400 70, glycerine 10; Wherein, the general flavone content 〉=18wt% in the propolis, ginkgo biloba p.e contains the 24wt% general flavone.
A kind of preparation method of bee glue soft capsule, this method may further comprise the steps:
(1) makes utricule: be the utricule raw material with gelatin, glycerine and pure water, glycerine and pure water are injected in the glue pot, be heated with stirring to 70 ℃, add gelatin again, continue to stir final vacuum deaeration in 20 minutes, make and obtain utricule, wherein the weight ratio of gelatin, glycerine and pure water is 20: 10: 20;
(2) preparation content: at first propolis is pulverized back and PEG400 and glycerine and is fully mixed 20min down at 70 ℃, and then add ginkgo biloba p.e and fully mix 60min, colloid mill 3 times, the content that obtains mixing;
(3) preparation capsule: content and step (1) making of step (2) preparation are obtained utricule process pelleting, finalize the design, wash ball, drying, choose ball and packing step, prepare bee glue soft capsule, it is 500 milligrams that every capsules contains content.
Embodiment 5
A kind of bee glue soft capsule comprises the content in utricule and the inclosure utricule, and content is made by the raw material of following component and weight percent content: propolis 30, ginkgo biloba p.e 10, PEG400 50, glycerine 10; General flavone content 〉=18wt% in the propolis, ginkgo biloba p.e contains the 24wt% general flavone.
A kind of preparation method of bee glue soft capsule, this method may further comprise the steps:
(1) makes utricule: be the utricule raw material with gelatin, glycerine and pure water, glycerine and pure water are injected in the glue pot, be heated with stirring to 65 ℃, add gelatin again, continue to stir final vacuum deaeration in 25 minutes, make and obtain utricule, wherein the weight ratio of gelatin, glycerine and pure water is 10: 5: 5;
(2) preparation content: at first propolis is pulverized back and PEG400 and glycerine and is fully mixed 40min down at 50 ℃, and then add ginkgo biloba p.e and fully mix 30min, colloid mill 1 time, the content that obtains mixing;
(3) preparation capsule: content and step (1) making of step (2) preparation are obtained utricule process pelleting, finalize the design, wash ball, drying, choose ball and packing step, prepare bee glue soft capsule, it is 500 milligrams that every capsules contains content.
Claims (6)
1. a bee glue soft capsule comprises the content in utricule and the inclosure utricule, it is characterized in that described content is made by the raw material of following component and weight percent content:
Propolis 18～50;
Ginkgo biloba p.e 1～20;
PEG400 40～70;
Glycerine 5～20.
2. a kind of bee glue soft capsule according to claim 1 is characterized in that, described content is made by the raw material of following component and weight percent content:
Propolis 30;
Ginkgo biloba p.e 10;
PEG400 50;
Glycerine 10.
3. a kind of bee glue soft capsule according to claim 1 is characterized in that, the general flavone content 〉=18wt% in the described propolis.
4. a kind of bee glue soft capsule according to claim 1 is characterized in that, described ginkgo biloba p.e contains the 24wt% general flavone.
5. the preparation method of a bee glue soft capsule as claimed in claim 1 or 2 is characterized in that, this method may further comprise the steps:
(1) makes utricule: be the utricule raw material with gelatin, glycerine and pure water, glycerine and pure water are injected in the glue pot, be heated with stirring to 60～70 ℃, add gelatin again, continue to stir final vacuum deaeration in 20-30 minute, make obtaining utricule;
(2) preparation content: at first propolis is pulverized back and PEG400 and glycerine and is fully mixed 20～40min down at 50～70 ℃, and then add ginkgo biloba p.e and fully mix 30～60min, colloid mill 1～3 time, the content that obtains mixing;
(3) preparation capsule: content and step (1) making of step (2) preparation are obtained utricule process pelleting, finalize the design, wash ball, drying, choose ball and packing step, prepare bee glue soft capsule.
6. the preparation method of a kind of bee glue soft capsule according to claim 5 is characterized in that, the weight ratio of gelatin, glycerine and pure water is 5～20: 1～10 in the step (1): 5～20.
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| CN201310116947.4A CN103190621B (en) | 2013-04-03 | 2013-04-03 | Propolis soft capsule and preparation method thereof |
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| CN103815395A (en) * | 2013-12-25 | 2014-05-28 | 伊犁百信草原蜂业有限责任公司 | Propolis-safflower oil soft capsule and preparation method thereof |
| CN104585574A (en) * | 2015-01-07 | 2015-05-06 | 北京蜜蜂堂生物医药股份有限公司 | Propolis soft capsule product and preparation method thereof |
| CN105079044A (en) * | 2015-09-23 | 2015-11-25 | 李�杰 | Preparation method of soft capsules containing Chinese caterpillar funguses |
| CN105962356A (en) * | 2016-05-03 | 2016-09-28 | 宣城柏维力生物工程有限公司 | Health-care propolis soft capsules and preparation method thereof |
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| CN106491653A (en) * | 2016-11-02 | 2017-03-15 | 深圳市荣格保健品有限公司 | A kind of bee glue soft capsule and preparation method thereof |
| CN106722612A (en) * | 2016-12-02 | 2017-05-31 | 芜湖市诺康生物科技有限公司 | A kind of bee glue soft capsule and preparation method thereof |
| CN108272832A (en) * | 2018-04-10 | 2018-07-13 | 辽宁仙草堂药业有限公司 | A kind of ginkgo leaf soft capsule and preparation method thereof |
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| CN105962356A (en) * | 2016-05-03 | 2016-09-28 | 宣城柏维力生物工程有限公司 | Health-care propolis soft capsules and preparation method thereof |
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| CN106491653A (en) * | 2016-11-02 | 2017-03-15 | 深圳市荣格保健品有限公司 | A kind of bee glue soft capsule and preparation method thereof |
| CN106722612A (en) * | 2016-12-02 | 2017-05-31 | 芜湖市诺康生物科技有限公司 | A kind of bee glue soft capsule and preparation method thereof |
| CN108272832A (en) * | 2018-04-10 | 2018-07-13 | 辽宁仙草堂药业有限公司 | A kind of ginkgo leaf soft capsule and preparation method thereof |
| CN113473964A (en) * | 2019-02-19 | 2021-10-01 | 阿皮奥迪科斯技术有限公司 | Propolis liquid extract and preparation and application thereof |
| CN113473964B (en) * | 2019-02-19 | 2023-06-23 | 阿皮奥迪科斯技术有限公司 | Propolis liquid extract, preparation and use thereof |
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