CN103189511B - 利用siRNA导入的新型hiPSC制作法 - Google Patents

利用siRNA导入的新型hiPSC制作法 Download PDF

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CN103189511B
CN103189511B CN201180043814.8A CN201180043814A CN103189511B CN 103189511 B CN103189511 B CN 103189511B CN 201180043814 A CN201180043814 A CN 201180043814A CN 103189511 B CN103189511 B CN 103189511B
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cells
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CN103189511A (zh
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三浦典正
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Tottori University NUC
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CN201180043814.8A 2010-07-12 2011-06-28 利用siRNA导入的新型hiPSC制作法 Expired - Fee Related CN103189511B (zh)

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Application Number Priority Date Filing Date Title
JP2010-158194 2010-07-12
JP2010-158193 2010-07-12
JP2010158193 2010-07-12
JP2010158194 2010-07-12
JP2010-158192 2010-07-12
JP2010158192 2010-07-12
PCT/JP2011/064846 WO2012008301A1 (ja) 2010-07-12 2011-06-28 ｓｉＲＮＡ導入による新規ｈｉＰＳＣ作製法

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CN103189511B true CN103189511B (zh) 2016-10-12

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CN201180043810XA Pending CN103097535A (zh) 2010-07-12 2011-06-28 利用miRNA导入的新型hiPSC制作法

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Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103189511B (zh) 2010-07-12 2016-10-12 国立大学法人鸟取大学 利用siRNA导入的新型hiPSC制作法
EP2686418A4 (en) 2011-03-17 2015-04-22 Minerva Biotechnologies Corp METHOD FOR THE PRODUCTION OF PLURIPOTENTAL STEM CELLS
EP2768945B1 (en) 2011-10-17 2022-01-05 Minerva Biotechnologies Corporation Media for stem cell proliferation and induction
EP2844744A2 (en) * 2012-02-22 2015-03-11 Brainstem Biotec Ltd. MicroRNAS FOR THE GENERATION OF ASTROCYTES
JP6748430B2 (ja) * 2012-07-13 2020-09-02 ミネルバ バイオテクノロジーズ コーポレーション より未分化状態への細胞の誘導方法
JPWO2015030149A1 (ja) * 2013-08-29 2017-03-02 国立大学法人鳥取大学 細胞のアンチエイジングに関連する生体分子群
TW201610152A (zh) * 2013-11-22 2016-03-16 國立臺灣大學 微ｒｎａ１４６－ａ及其於診斷、治療及預防小核醣核酸病毒感染之用途及微ｒｎａ１４６－ａ拮抗劑
CN104109670B (zh) * 2014-03-12 2018-01-05 首都医科大学附属北京安定医院 一种双链siRNA分子及其应用
AU2015278699B2 (en) 2014-06-27 2019-10-31 Rhizen Pharmaceuticals Sa Substituted chromene derivatives as selective dual inhibitors of PI3 delta and gamma protein kinases
US10806748B2 (en) 2015-04-24 2020-10-20 The Johns Hopkins University Compositions and methods related to characterizing proviral reservoirs
US20180311270A1 (en) * 2015-10-30 2018-11-01 Norimasa Miura Rna molecule for repairing dna damage
KR102011336B1 (ko) * 2015-12-31 2019-08-16 인터올리고 주식회사 약물 전달 및 안정화를 위한 복합체 및 그 제조방법
WO2018203553A1 (ja) * 2017-05-02 2018-11-08 株式会社ペジィー・ファーマ 分化細胞の品質改善方法
WO2023128862A1 (en) * 2021-12-29 2023-07-06 Livius Pte. Ltd. Method for repairing hair cycle-related genes and method for treating hair cycle-related diseases using mir-520d-5p
JP2023149907A (ja) * 2022-03-31 2023-10-16 株式会社島津製作所 微生物情報の提供方法および微生物検査システム

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006112239A1 (ja) * 2005-04-15 2006-10-26 National University Corporation Tottori University ｈＴＥＲＴ発現調節遺伝子
CN101333524A (zh) * 2008-06-25 2008-12-31 中山大学 一个小分子非编码RNA基因hsa-miR-101及其抗肿瘤用途

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2467258A1 (en) * 2001-11-15 2003-05-22 Kyowa Hakko Kogyo Co., Ltd. Agent for inducing embryonic stem cell to ectodermal cell, method for obtaining the same and use of the same.
AU2003270734A1 (en) 2002-09-16 2004-04-30 University Of Southern California Rna-mediated gene modulation
JP2006519008A (ja) 2003-02-10 2006-08-24 独立行政法人産業技術総合研究所 哺乳動物細胞の調節
US9453219B2 (en) * 2003-05-15 2016-09-27 Mello Biotech Taiwan Co., Ltd. Cosmetic designs and products using intronic RNA
CA2857881A1 (en) * 2004-11-12 2006-12-28 Asuragen, Inc. Methods and compositions involving mirna and mirna inhibitor molecules
MX2007009417A (es) 2005-02-04 2007-08-17 Univ Auburn Sistema de suministro de farmaco de contacto.
PL2002003T3 (pl) * 2005-05-27 2016-06-30 Ospedale San Raffaele Srl Wektor genetyczny zawierający mi-RNA
WO2006137514A1 (ja) 2005-06-23 2006-12-28 Locomogene, Inc. シノビオリンの発現もしくは機能阻害物質を有効成分とする癌治療剤、および癌治療剤のスクリーニング方法
BRPI0619794B8 (pt) 2005-12-13 2022-06-14 Univ Kyoto Uso de um fator de reprogramação, agente para a preparação de uma célula-tronco pluripotente induzida a partir de uma célula somática e métodos para preparar uma célula- tronco pluripotente induzida método e para preparar uma célula somática e uso de células-tronco pluripotentes induzidas
EP2487258B1 (en) * 2006-01-05 2014-10-01 The Ohio State University Research Foundation MicroRNA-based methods for the diagnosis of colon, pancreas and stomach cancer
CA2648132C (en) 2006-04-03 2019-05-28 Santaris Pharma A/S Pharmaceutical composition comprising anti-mirna antisense oligonucleotides
EP2217248B1 (en) * 2007-10-29 2016-11-30 Regulus Therapeutics Inc. Targeting micrornas for the treatment of liver cancer
AU2008297024B2 (en) 2007-10-31 2014-08-28 Kyoto University Nuclear reprogramming method
JP5558097B2 (ja) 2007-12-10 2014-07-23 国立大学法人京都大学 効率的な核初期化方法
WO2009082817A1 (en) * 2007-12-27 2009-07-09 Protiva Biotherapeutics, Inc. Silencing of polo-like kinase expression using interfering rna
WO2009091659A2 (en) * 2008-01-16 2009-07-23 Shi-Lung Lin Generation of tumor-free embryonic stem-like pluripotent cells using inducible recombinant rna agents
CN101392251B (zh) * 2008-11-03 2015-07-22 清华大学深圳研究生院 能诱导干细胞向成骨细胞分化的微小rna及其应用
JP2010120881A (ja) * 2008-11-19 2010-06-03 Keio Gijuku ヒト癌タンパク質ＭＤＭ２とヒト癌抑制タンパク質ｐ５３との相互作用阻害ペプチド及びその使用
CN103189511B (zh) 2010-07-12 2016-10-12 国立大学法人鸟取大学 利用siRNA导入的新型hiPSC制作法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006112239A1 (ja) * 2005-04-15 2006-10-26 National University Corporation Tottori University ｈＴＥＲＴ発現調節遺伝子
CN101333524A (zh) * 2008-06-25 2008-12-31 中山大学 一个小分子非编码RNA基因hsa-miR-101及其抗肿瘤用途

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
A noncoding RNA gene on chromosome 10p15.3 may function upstream of hTERT;Norimasa Miura等;《BMC Molecular Biology》;20090202;第10卷(第5期);第5页 *

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EP2594643A1 (en) 2013-05-22
US9790491B2 (en) 2017-10-17
US20130190389A1 (en) 2013-07-25
JP2013046616A (ja) 2013-03-07
JPWO2012008301A1 (ja) 2013-09-09
EP2594643B1 (en) 2017-04-12
JPWO2012008302A1 (ja) 2013-09-09
EP2594644A1 (en) 2013-05-22
CN103097535A (zh) 2013-05-08
WO2012008301A1 (ja) 2012-01-19
CN103189511A (zh) 2013-07-03
JP2013034479A (ja) 2013-02-21
JP5099570B2 (ja) 2012-12-19
US20130184335A1 (en) 2013-07-18
EP2594644A4 (en) 2014-12-24
US9476041B2 (en) 2016-10-25
JP2014097059A (ja) 2014-05-29
JP5845493B2 (ja) 2016-01-20
EP2594643A4 (en) 2014-12-24
JP5099571B2 (ja) 2012-12-19
EP2594644B1 (en) 2016-08-10
WO2012008302A1 (ja) 2012-01-19

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