CN103175828A - Detection device for detecting free heme in sputum - Google Patents
Detection device for detecting free heme in sputum Download PDFInfo
- Publication number
- CN103175828A CN103175828A CN2013100875761A CN201310087576A CN103175828A CN 103175828 A CN103175828 A CN 103175828A CN 2013100875761 A CN2013100875761 A CN 2013100875761A CN 201310087576 A CN201310087576 A CN 201310087576A CN 103175828 A CN103175828 A CN 103175828A
- Authority
- CN
- China
- Prior art keywords
- tube
- reagent
- reagent tube
- pick
- pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 206010036790 Productive cough Diseases 0.000 title claims abstract description 18
- 210000003802 sputum Anatomy 0.000 title claims abstract description 18
- 208000024794 sputum Diseases 0.000 title claims abstract description 18
- 238000001514 detection method Methods 0.000 title abstract description 9
- 150000003278 haem Chemical class 0.000 title abstract 3
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 68
- 238000002156 mixing Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 4
- 239000000243 solution Substances 0.000 claims description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 2
- 241000973497 Siphonognathus argyrophanes Species 0.000 claims description 2
- 239000000872 buffer Substances 0.000 claims description 2
- 239000007979 citrate buffer Substances 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 description 5
- 240000007643 Phytolacca americana Species 0.000 description 1
- 210000000621 bronchi Anatomy 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a detection device for detecting free heme in sputum. The detection device comprises a specimen tube, an agent tube and a pointed drip tube; the agent tube comprises an upper first agent tube and a lower second agent tube, wherein the bottom of the first agent tube is in a shape of a funnel; a tube port is formed in the bottom of the first agent tube, and the tube port is sealed by a tinfoil; the wall of the first agent tube downwards extends to form a connecting ring; and a tube port of the second agent tube is in seal connection with the connecting ring. The detection device provided by the invention is simple and practical, and low in cost, and can quickly screen whether the sputum contains the free heme or not.
Description
Technical field
The present invention relates to a kind of pick-up unit, particularly relate to a kind of pick-up unit that detects the free protoheme of sputum.
Background technology
Free protoheme in sputum is to be discharged by the hemoprotein of lung and bronchus cell to produce.The content of its free protoheme is few, the naked eyes non-availability is seen, generally need to detect by advanced person's instrument and analytical chemistry method, expense is high, cycle is long, determine fast whether contain free protoheme in sputum, become a problem of needing solution badly so invent detection method a kind of simple to operate, with low cost and pick-up unit.
Summary of the invention
In order to solve the problem that the method expense that detects free protoheme in sputum in prior art is high, the cycle is long, the invention provides a kind of pick-up unit that detects the free protoheme of sputum, of the present invention highly sensitive, with low cost, can detect fast the free protoheme in sputum.
The invention provides a kind of pick-up unit that detects the free protoheme of sputum.
Described pick-up unit comprises specimen tube, Reagent Tube and tip dropper, described Reagent Tube comprises the second Reagent Tube of superposed the first Reagent Tube and bottom, be funnel-form at the bottom of the pipe of described the first Reagent Tube, bottom, the pipe end is provided with the mouth of pipe, be sealed with tinfoil paper on the mouth of pipe, the tube wall of the first Reagent Tube is extended with abutment ring downwards, and the mouth of pipe and the abutment ring of described the second Reagent Tube are tightly connected.
Further, it is 7.4 tris-HCI buffer 2ml that described specimen tube is built-in with pH value, and described the first Reagent Tube is built-in with reagent A 1ml, is built-in with reagent B1ml in described the second Reagent Tube; Described reagent A compound method is as follows: get 3,3', it is that 7.4 citrate buffer solution 100ml mixings form that 5,5'-tetramethyl benzidine 50mg is dissolved in PH; Described reagent B is 3% superoxol.
Further, described abutment ring is threaded with being connected to of the second Reagent Tube.
Further, the material of the body of described the first Reagent Tube is elastoplast.
Further, described specimen tube diameter 1.8 cm, high 1.6cm.
Further, described Reagent Tube diameter 1.8 cm.
Further, described the first high 1cm of Reagent Tube, pipe bottom tube mouth diameter 0.5cm.
Further, described the second high 1.6cm of Reagent Tube.
Further, the long 3cm of described tip dropper, volume is 1ml.
Pick-up unit provided by the invention, simple and practical, with low cost, examination fast goes out the sample that do not contain free protoheme in sputum, contain the sample of free protoheme and may contain the sample of free protoheme, prepares for further checking; The detectable minimum free protoheme concentration of the present invention can reach 0.5ng/ml, and is highly sensitive.
Description of drawings
Fig. 1 is the structural representation of the second Reagent Tube.
Fig. 2 is the structural representation of the first Reagent Tube.
Fig. 3 is the structural representation of tip dropper.
Fig. 4 is the structural representation of specimen tube.
1, the second Reagent Tube; 2, the first Reagent Tube lower nozzle; 3, the first Reagent Tube; 4, abutment ring; 5, the first Reagent Tube top cover; 6, tip dropper; 7, specimen tube; 8, tinfoil paper.
Embodiment
The present invention is described further below in conjunction with accompanying drawing.
As shown in Fig. 1-4, pick-up unit provided by the invention comprises specimen tube 7, Reagent Tube and tip dropper 6, described Reagent Tube comprises the second Reagent Tube 1 of superposed the first Reagent Tube 3 and bottom, be funnel-form at the bottom of the pipe of described the first Reagent Tube 3, bottom, the pipe end is provided with the first Reagent Tube lower nozzle 2, the tube wall that is sealed with tinfoil paper 8, the first Reagent Tubes 3 on lower nozzle 2 is extended with abutment ring 4 downwards, and the mouth of pipe of described the second Reagent Tube 1 and abutment ring 4 are tightly connected.
Using method of the present invention is as follows:
(1) get specimen tube 7, ask the person under inspection leave and take sputum and with the damping fluid mixing.
(2) open Reagent Tube the first Reagent Tube top cover 5, draw this liquid of sputum sample 1ml with tip dropper 6, drop to the first Reagent Tube 3, mixing.
(3) poke again the tinfoil paper 8 of the first Reagent Tube lower nozzle 2 of the first Reagent Tube 3 bottoms with tip dropper 6, slightly press at the first Reagent Tube 3 tube walls, make that in sample liquid in the first Reagent Tube 3 and the first Reagent Tube, original reagent A flows into the second Reagent Tube 1.If reagent fails all to enter the second Reagent Tube 1, press again 2 ~ 3 times after removing the pressure return-air after can pressing, till flowing into the second Reagent Tube 1 to sample liquid and reagent A.Build the first Reagent Tube top cover 5, shake gently, make sample liquid, reagent A and reagent B three mixing.
Observe the change color of the second reagent liquid in pipe in (4) 2 minutes, compare with colorimetric card and record testing result.
Testing result is judged as follows:
Negative if do not develop the color, illustrate in sepage without free protoheme; If show that light green blueness to light blue, may contain free protoheme, need regularly check; Contain free protoheme in sepage if show blue the explanation to mazarine.If show that at once mazarine and moment transfer buff or brownish red to, indicate and organize hemorrhagely, also should be considered as strong positive.
Experimental data:
Get respectively and detect as not developing the color, show that light green blueness is extremely light blue, blue each three examples of extremely navy blue sample, carry out the detection of protoheme with mass spectrometer, testing result is as follows: do not develop the color and all do not survey and protoheme; Light green color to light blue is: 0.5 ng/ml, 1.7ng/ml, 4.3 ng/ml; Blueness to mazarine is 20ng/ml, 2.5 μ g/ml, 52 μ g/Lml.
Can find out by above data, the free sputum sample of content of hemachrome more than 0.5ng/ml used the judgement that this detection method just can be preliminary, the free sputum sample of content of hemachrome more than 20ng/ml uses this detection method just can be defined as containing free protoheme in sputum, so this detection method is highly sensitive.
Claims (9)
1. pick-up unit that detects the free protoheme of sputum, it is characterized in that, described pick-up unit comprises specimen tube, Reagent Tube and tip dropper, described Reagent Tube comprises the second Reagent Tube of superposed the first Reagent Tube and bottom, be funnel-form at the bottom of the pipe of described the first Reagent Tube, pipe is provided with the mouth of pipe at the end, is sealed with tinfoil paper on the mouth of pipe, the tube wall of the first Reagent Tube is extended with abutment ring downwards, and the mouth of pipe and the abutment ring of described the second Reagent Tube are tightly connected.
2. pick-up unit according to claim 1, it is characterized in that, it is 7.4 tris-HCI buffer 2ml that described specimen tube is built-in with pH value, and described the first Reagent Tube is built-in with reagent A 1ml, is built-in with reagent B1ml in described the second Reagent Tube; Described reagent A compound method is as follows: get 3,3', it is that 7.4 citrate buffer solution 100ml mixings form that 5,5'-tetramethyl benzidine 50mg is dissolved in PH; Described reagent B is 3% superoxol.
3. pick-up unit according to claim 1, is characterized in that, described abutment ring is threaded with being connected to of the second Reagent Tube.
4. pick-up unit according to claim 1, is characterized in that, the material of the body of described the first Reagent Tube is elastoplast.
5. pick-up unit according to claim 1, is characterized in that, described specimen tube diameter 1.8 cm, high 1.6cm.
6. pick-up unit according to claim 1, is characterized in that, described Reagent Tube diameter 1.8 cm.
7. according to claim 1 or 4 described pick-up units, is characterized in that, described the first high 1cm of Reagent Tube, pipe bottom tube mouth diameter 0.5cm.
8. according to claim 1 or 3 described pick-up units, is characterized in that, described the second high 1.6cm of Reagent Tube.
9. pick-up unit according to claim 1, is characterized in that, the long 3cm of described tip dropper, and volume is 1ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310087576.1A CN103175828B (en) | 2013-03-19 | 2013-03-19 | A kind of detection means for detecting free heme in sputum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310087576.1A CN103175828B (en) | 2013-03-19 | 2013-03-19 | A kind of detection means for detecting free heme in sputum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103175828A true CN103175828A (en) | 2013-06-26 |
| CN103175828B CN103175828B (en) | 2017-12-22 |
Family
ID=48635831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310087576.1A Expired - Fee Related CN103175828B (en) | 2013-03-19 | 2013-03-19 | A kind of detection means for detecting free heme in sputum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103175828B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104306029A (en) * | 2014-11-04 | 2015-01-28 | 温鑫 | Developing tissue exudate sampling and detection device |
| WO2016004551A1 (en) * | 2014-07-11 | 2016-01-14 | 青岛千士医疗科技有限公司 | Method for detecting mitochondria damage of epithelial cells of bronchia/lung |
| CN108982498A (en) * | 2018-07-18 | 2018-12-11 | 浙江今复康生物科技有限公司 | Detect the method and device of airway surface liquid pH value indirectly using sputum |
| CN112326641A (en) * | 2020-07-29 | 2021-02-05 | 浙江今复康生物科技有限公司 | Method and kit for rapidly identifying sputum components from expectorated sample |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201476980U (en) * | 2009-08-20 | 2010-05-19 | 苏州浦隆生物有限公司 | Device for sampling and testing |
| JP2012215440A (en) * | 2011-03-31 | 2012-11-08 | Tokyo Gas Co Ltd | Method and device to detect cyclohexene in fuel gas |
| CN202614684U (en) * | 2012-06-12 | 2012-12-19 | 北京捷乐生物科技有限公司 | Device for detecting |
| CN102928424A (en) * | 2012-10-30 | 2013-02-13 | 青岛贝尔奥生物科技有限公司 | Method and detection kit for detecting cervical fluid heme |
| CN203164127U (en) * | 2013-03-19 | 2013-08-28 | 青岛千士医疗科技有限公司 | Device for detecting sputum free haemoglobin |
-
2013
- 2013-03-19 CN CN201310087576.1A patent/CN103175828B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201476980U (en) * | 2009-08-20 | 2010-05-19 | 苏州浦隆生物有限公司 | Device for sampling and testing |
| JP2012215440A (en) * | 2011-03-31 | 2012-11-08 | Tokyo Gas Co Ltd | Method and device to detect cyclohexene in fuel gas |
| CN202614684U (en) * | 2012-06-12 | 2012-12-19 | 北京捷乐生物科技有限公司 | Device for detecting |
| CN102928424A (en) * | 2012-10-30 | 2013-02-13 | 青岛贝尔奥生物科技有限公司 | Method and detection kit for detecting cervical fluid heme |
| CN203164127U (en) * | 2013-03-19 | 2013-08-28 | 青岛千士医疗科技有限公司 | Device for detecting sputum free haemoglobin |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016004551A1 (en) * | 2014-07-11 | 2016-01-14 | 青岛千士医疗科技有限公司 | Method for detecting mitochondria damage of epithelial cells of bronchia/lung |
| CN104306029A (en) * | 2014-11-04 | 2015-01-28 | 温鑫 | Developing tissue exudate sampling and detection device |
| CN108982498A (en) * | 2018-07-18 | 2018-12-11 | 浙江今复康生物科技有限公司 | Detect the method and device of airway surface liquid pH value indirectly using sputum |
| CN112326641A (en) * | 2020-07-29 | 2021-02-05 | 浙江今复康生物科技有限公司 | Method and kit for rapidly identifying sputum components from expectorated sample |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103175828B (en) | 2017-12-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103175828A (en) | Detection device for detecting free heme in sputum | |
| CN109444070A (en) | Oil content all automatic measurement instrument in water | |
| CN203164127U (en) | Device for detecting sputum free haemoglobin | |
| CN103267756B (en) | Method and detection box for detecting nasopharynx exudate free heme | |
| CN106841483A (en) | A kind of sample introduction separator of ping-pong structure | |
| CN102928424A (en) | Method and detection kit for detecting cervical fluid heme | |
| CN207667679U (en) | A kind of closed integrated detection reagent pipe | |
| CN104568511A (en) | Medical examination sampling device | |
| CN104111150A (en) | Device for detecting airtightness of container detection port and detection method | |
| CN103453930B (en) | For multiple testing integrity of small-area peg-top type hydrophobic air filters devices | |
| CN105136788A (en) | Digital analyzer of saliva or urine specimen | |
| CN204807439U (en) | Be applicable to and water funkly be afraid of that oxygen compound carries out ultraviolet cell for fluorescence detection | |
| CN204855201U (en) | Honey sampling device | |
| CN104502481A (en) | Method for detecting vomitoxin and zearalenone in grains and feeds | |
| CN206479499U (en) | A kind of sample introduction separator of ping-pong structure | |
| CN209542626U (en) | A kind of Seperated multiple channel chromatographic apparatus | |
| CN104209154A (en) | Detection test pipe and use method thereof | |
| CN207964365U (en) | A kind of water-sampling device | |
| CN206924774U (en) | Micro flow control chip device | |
| CN201181280Y (en) | Portable jet fuel suspending water fast detecting instrument | |
| CN205538682U (en) | Fluorescence flow -through cell of terminal surface photic | |
| CN204807558U (en) | Be applied to buffer solution application of sample ware of immunity chromatography detection technique | |
| CN107764881B (en) | Portable blood detection kit device based on electrochemical method | |
| CN205049516U (en) | Adopt unicellular gel electrophoresis to detect kit of DNA chain interruption | |
| CN202725038U (en) | Blood corpuscle and blood plasma separating device for trace blood collection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200928 Address after: Unit 102, unit 2, No. 63, Dunhua Road, Qingdao, Shandong Province Patentee after: Cui Zilun Address before: 266071 Shandong Province, Qingdao city Hongkong Road No. 9 - Qingdao science and Technology Park D third floor Patentee before: QINGDAO KING'S MEDICAL SCIENCE AND TECHNOLOGY Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171222 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |