CN103173430A - Complex enzyme for processing red dates and using method thereof - Google Patents
Complex enzyme for processing red dates and using method thereof Download PDFInfo
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- CN103173430A CN103173430A CN2013100956241A CN201310095624A CN103173430A CN 103173430 A CN103173430 A CN 103173430A CN 2013100956241 A CN2013100956241 A CN 2013100956241A CN 201310095624 A CN201310095624 A CN 201310095624A CN 103173430 A CN103173430 A CN 103173430A
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Abstract
The invention aims at providing a complex enzyme for processing red dates. The complex enzyme is prepared from acidic pectinase, acidic cellulose, acidic xylanase, arabanase and beta-glucosaccharase. All the components in the liquid complex enzyme disclosed by the invention can generate a synergistic effect; the ingredients of the complicated cell walls of the red dates are fully decomposed; the effect is efficient and complete; the material conversion rate is greatly improved by combining with a new technology of processing red date concentrate juice by adopting an enzyme method, and the production efficiency and the product quality are improved. By applying the new technology of processing of the red date concentrate juice by adopting the enzyme method, the conversion rate of a red dates material is improved by over 20%; the ultra-filtration cleaning time of an ultra-filtration apparatus is prolonged to two days once from once or twice in a day; the fluency of the processing technology is greatly improved, so as to greatly improve the production efficiency; the heating softening temperature is reduced to be at 70 DEG C from 90 DEG C; the energy consumption is greatly reduced; and the problems that the product red date juice has an obvious cooking taste, dark and poor color, and serious loss of the vitamin C are also solved.
Description
Technical field
The invention belongs to the Food enzyme technical field, be specifically related to a kind of prozyme and using method thereof for red date processing.
Background technology
Red date claims again date, in China, more than 3,000 year cultivation history has been arranged, and as the abundantest country of jujube resource, so far, the cultivated area of the red date more than 95% and output all concentrate on China in the world.Red date nutrient is abundant, fragrant and sweet crisp strongly fragrant, the thin meat of skin is thick, title with " woody grain ", its rich vitamin, protein, carbohydrate, soap are general, mineral substance, Flavonoid substances and alkaloid etc., are a kind of dietotherapeutic fruits of high-quality, have the title of " active vitamin ball, Da Bu Wan ".The red date smell is flat, nontoxic, sweet, has invigorating the spleen and regulating the stomach, promoting blood flow to regulate menstruation, tranquilizing by nourishing the heart, and the antidiarrheal of promoting the production of body fluid, anti-inflammatory analgetic and the effect that relaxes the property of medicine are applicable to weakness of the spleen and the stomach, fatigue and weakness, deficiency of vital energy deficiency, the purple paralysis of supersensitivity, insomnia etc.Studies show that, red date can reduce the cholesterol in serum, and the protection liver increases total serum protein and albumin, muscle strength reinforcing, anti-allergic, the effects such as anticancer propagation.
Red date is that some other medicine or healthcare products institute are irreplaceable for the health care of human body, is the fruit resource with dietotherapeutic of exploitation future.In recent years, red date intensive processing industry develops rapidly, the emphasis that is processed into the red dates industry development of jujube juice, inspissated juice (slurry) and red date wine, vinegar.The red date intensive processing products such as jujube juice, wine, vinegar have not only kept the original nutritive ingredient of red date and local flavor, are easy to be absorbed by the body, and instant, supply cycle is longer, liked by consumers in general.But also there are a lot of technical barriers in the intensive processing field of red date, wherein feed stock conversion is low, and the inspissated juice complete processing has some setbacks, final product quality is unstable, obvious boiling flavor, color and luster are arranged, and secretly the loss of poor, vitamins C is serious, very easily forming muddiness or precipitation etc. in storage, to affect the problem of inspissated juice local flavor and quality particularly outstanding.
Summary of the invention
The purpose of this invention is to provide a kind of prozyme and using method thereof for red date processing, this prozyme can obviously improve the red date feed stock conversion, improve the fluency of red date inspissated juice complete processing, thereby enhance productivity, reduce extraction temperature, solving finished product jujube juice has obvious boiling flavor, the color and luster problems such as secretly poor, vitamins C loss is serious, and then improves the quality stability of finished product jujube juice, improves its local flavor and quality.
One aspect of the invention provides a kind of prozyme, and this prozyme is comprised of acid pectase, acidic cellulase, acidic xylanase, arabanase and beta-glucosidase.
Prozyme of the present invention is liquid dosage form, and in prozyme, the enzyme of each component content alive is respectively: acid pectase 10000～30000u/ml; Acidic cellulase 7000～10000u/ml; Acidic xylanase 40000～60000u/ml; Arabanase 5～200u/ml; Beta-glucosidase 0.5～100u/ml.
Another aspect of the present invention provides the application of above-mentioned prozyme in red date processing, specifically comprises the steps:
1) with red date with warm water soaking after, reheat softeningly, then making beating obtains red date slurry;
2) addition of pressing the dried jujube of 0.1-2L/ ton adds prozyme to the red date slurry, stirs, and carries out Enzymolysis under 40-55 ℃ of constant temperature, extraction time 45-150min;
3) red date after enzymolysis slurry is sieved, centrifugal, ultrafiltration, obtain clarifying jujube juice;
4) clarifying jujube juice is concentrated, obtaining soluble solid is the concentrated clear juice of red date of 65-70 ° of Brix;
5) with the concentrated clear juice sterilization of red date, carry out sterile filling, obtain jujube juice.
Wherein in step 1), warm water soaking is with 35 ℃ of left and right warm water soaking 24h; Thermoplastic is thermoplastic 15 minutes under 70 ℃ of constant temperatures.
Red date processing dedicated liquid prozyme provided by the invention, use simple, convenient, and in prozyme, each endoenzyme component produces synergy, complex cell wall composition to red date decomposes fully, effect is efficient, complete, use in conjunction with red date inspissated juice enzymatic processing novel process of the present invention, greatly improved feed stock conversion, improved production efficiency and quality product.Use red date inspissated juice enzymatic processing novel process of the present invention, can make the transformation efficiency of red date raw material improve more than 20%; The wash number of ultrafiltration apparatus extends to two days once by one day 1-2 time, has greatly improved the fluency of jujube juice processing technique, thereby has greatly improved production efficiency; And the thermoplastic temperature is dropped to 70 ℃ by 90 ℃, not only greatly reduce energy consumption, also having solved finished product jujube juice the problem such as has obvious boiling flavor, a color and luster secretly poor, ascorbic loss is serious, has greatly improved finished product jujube juice quality stability, has improved its local flavor and quality.The present invention is the Successful Practice of the single enzyme of reasonable composite use, can promote low cost, the High-efficient Production of red date intensive processing industry.
Embodiment
The present invention is raw materials used all can be selected from commercially available arbitrary product with equipment, and for example acid pectase, acidic cellulase, acidic xylanase can be selected from Qingdao Weilan Biology Group Co., Ltd., are liquid product; Arabanase can available from the biochemical company limited in Baofeng, Shanghai, be solid phase prod; Beta-glucosidase can available from Japan sky wild A Manuo zymin company, be solid phase prod.
Embodiment 1
The present invention is used for the liquid complex enzyme of red date intensive processing, and its each component and enzyme work are respectively: acid pectase 30000u/ml; Acidic cellulase 10000u/ml; Acidic xylanase 60000u/ml; Arabanase 5u/ml; Beta-glucosidase 0.5u/ml.
Its preparation method is as follows:
Acid pectase, acidic cellulase and three kinds of liquid enzymes of acidic xylanase are mixed, stir, be made into the mixed enzyme solution of three kinds of enzymes; Then add arabanase in mixed enzyme solution, stir; Add beta-glucosidase again in mixed enzyme solution, stir, namely obtain the aforesaid liquid prozyme.
Embodiment 2
Liquid complex enzyme, its each component and enzyme work are respectively: acid pectase 30000u/ml; Acidic cellulase 10000u/ml; Acidic xylanase 60000u/ml; Arabanase 200u/ml; Beta-glucosidase 100u/ml.
The preparation method is described with embodiment 1.
Embodiment 3
Be used for the liquid complex enzyme of red date intensive processing, its each component and enzyme work are respectively: acid pectase 20000u/ml; Acidic cellulase 8500u/ml; Acidic xylanase 50000u/ml; Arabanase 5u/ml; Beta-glucosidase 0.5u/ml.
The preparation method is described with embodiment 1.
Embodiment 4
A kind of liquid complex enzyme for the red date intensive processing, its each component and enzyme work are respectively: acid pectase 20000u/ml; Acidic cellulase 8500u/ml; Acidic xylanase 50000u/ml; Arabanase 200u/ml; Beta-glucosidase 100u/ml.
The preparation method is described with embodiment 1.
Embodiment 5
A kind of dedicated liquid prozyme for the red date intensive processing, its each component and enzyme work are respectively: acid pectase 20000u/ml; Acidic cellulase 8500u/ml; Acidic xylanase 50000u/ml; Arabanase 110u/ml; Beta-glucosidase 55u/ml.
The preparation method is described with embodiment 1.
Embodiment 6
A kind of dedicated liquid prozyme for the red date intensive processing, its each component and enzyme work are respectively: acid pectase 10000u/ml; Acidic cellulase 7000u/ml; Acidic xylanase 40000u/ml; Arabanase 5u/ml; Beta-glucosidase 0.5u/ml.
The preparation method is described with embodiment 1.
Embodiment 7
A kind of dedicated liquid prozyme for the red date intensive processing, its each component and enzyme work are respectively: acid pectase 10000u/ml; Acidic cellulase 7000u/ml; Acidic xylanase 40000u/ml; Arabanase 200u/ml; Beta-glucosidase 100u/ml.
The preparation method is described with embodiment 1.
Embodiment 8
Use the red date inspissated juice complete processing of liquid complex enzyme of the present invention:
Prozyme of the present invention includes following step in preparation red date liquid: one weighing one of choosing fruit cleans one, and to soak the thermoplastic one making beating one enzymolysis one centrifugal concentrated sterilization one of ultrafiltration one of sieving canned; Specific as follows:
Respectively A(is picked up from Xian County, Cangzhou) and the B(Linxian County, Shanxi Province) two kinds of raw material red dates select fruit (rejecting the fruit of rotting), clean, take appropriate, by 1:(3.5-6) bath raio (the preferred 1:5 of the present embodiment) added warm water soaking 24 hours.
Then be placed under 70 ℃ of constant temperatures thermoplastic 15 minutes, making beating obtains the red date slurry.
The enzyme addition of pressing the dried jujube of 0.1-2L/T (uses prozyme in embodiment 1 in the present embodiment, A dhdps enzyme addition 1L/T, B dhdps enzyme addition 1.2L/T) add liquid complex enzyme of the present invention, carry out Enzymolysis, the preferred 60min of Enzymolysis time 45-150min(the present embodiment under 40-55 ℃ of (preferred 48 ℃ of the present embodiment) constant temperature).
Sieve after enzymolysis is completed, centrifugal, isolated nuclei, skin obtain the turbid juice of red date, excessively obtain juice clearly after ultrafiltration, triple effect or five effects are concentrated to pol and adopt triple effect to concentrate in 65-70 ° of this experiment of Brix(, and pol is to reach 65 ° of Brix as standard) obtain inspissated juice.
A and two kinds of red date raw materials of B are selected fruit, weighing, cleaning, after soaking 24h by the 1:5 bath raio, be placed under 90 ℃ of constant temperatures thermoplastic 15 minutes, making beating, the red date that obtains slurry does not carry out enzymolysis, directly uses the hot water lixiviate as a control group.Control group also adopts same process to obtain inspissated juice.
The concentrated clear juice of above-mentioned red date carries out sterile filling through sterilization, obtains the concentrated clear juice finished product of red date.
Above-mentioned whole production process is monitored, and finished product carries out indices and detects.
Monitored results shows: not quite smooth without the control group raw material final treatment techniques that complex enzyme zymohydrolysis is processed, especially show in the ultrafiltration operation, and just begin ultra-filtration flux and only be 10m
3/ h, and it is very fast to descend, and moves less than the 12h ultra-filtration flux less than 5m
3/ h can not continue operation, must shut down and could continue after cleaning to move, and has had a strong impact on production efficiency; And the raw material final treatment techniques after processing through complex enzyme zymohydrolysis is very smooth, especially shows in the ultrafiltration operation, and the initial operating stage ultra-filtration flux surpasses 15m
3/ h, and it is slower to descend, and operation 15h ultra-filtration flux is still at 10m
3More than/h, more than minimum continuously-running 24h, greatly improved production efficiency.The enzymolysis group raw material softening temperature of precooking only needs 70 ℃, compares with 90 ℃ of control group and greatly reduces temperature, has not only saved the energy, and finished product do not have boiling flavor, vitamins C and other nutritive ingredient retention rate high, and its local flavor and quality have obviously promoted a lot.
Finished product index detected result such as following table:
Table 1 jujube juice index detected result
Annotate: except feed stock conversion, each index all is diluted to 11.5 ° of Brix at inspissated juice and measures.
Feed stock conversion (%) refers to that 1 ton of red date can transform the output of the concentrated jujube juice of producing.
As shown in Table 1, compare with control group, two kinds of red date feed stock conversions of the A after the liquid complex enzyme enzymolysis processing and B have all improved 20% left and right, can greatly reduce enterprise's production cost, have improved economic benefit.
In the research process of juice browning, colour is an important evaluation index.With respect to chemical analysis, the result of determination of colority is simpler, directly perceived, CIE-LAB colour system, and L (brightness), a (red green value) and b (champac value) can describe the colour-change of different fruit juice preferably.L represents black and white, and+expression is partially white, and-expression is partially dark.L is larger, illustrates that fruit juice is more limpid, transparent.A represents red green, and+expression is partially red, and-expression is partially green.A is larger, illustrates that juice color is redder.B represents champac, and+expression is partially yellow, and-expression is partially blue.B is larger, illustrates that juice color is yellow.As shown in Table 1, for A and two kinds of red date raw materials of B, not enzyme-added control group jujube juice color is partially dark, and partially red and yellow, and the jujube juice that obtains through complex enzyme zymohydrolysis technique is all more limpid, transparent than control group, better quality.Browning index A
420Also confirmed this point, browning index means the index of browning degree size, A
420Larger, the expression browning reaction is more serious; A
420Less, the expression browning degree is less.The treatment process and the prozyme that build in the present invention are used in conjunction with, and make the jujube juice browning degree of preparation very little.As shown in Table 1, the jujube juice browning index that obtains through complex enzyme zymohydrolysis technique for A and two kinds of red date raw materials of B is all less than control group, and browning degree is less, better quality.
Jujube juice contains abundant reduced form Vc, and it has good pharmaceutical use, such as, treatment vitamin C deficiency, anaemia etc.But reduced form Vc is extremely unstable, particularly easy oxidation browning under heating state.As shown in Table 1, for A and two kinds of red date raw materials of B, the jujube juice Vitamin C content that obtains through complex enzyme zymohydrolysis technique is all apparently higher than control group, and the nutritive ingredient storage rate is higher, and nutritive value is higher; And from sensory evaluation, for A and two kinds of red date raw materials of B, the jujube juice that obtains through complex enzyme zymohydrolysis technique all shows as the jujube aromatic flavour, there is no the boiling flavor, and control group is but that jujube fragrance is lighter, and obvious boiling flavor is arranged.
In sum, use liquid complex enzyme of the present invention and carry out enzymolysis processing, can make the transformation efficiency of red date raw material improve 20% left and right; The temperature of pulling an oar of precooking has reduced by 20 ℃, has both saved the energy, has increased again the brightness of finished product jujube juice, and finished product jujube juice has not had the boiling flavor yet, and the storage rate of vitamins C and other nutritive ingredient is high, and the local flavor of jujube juice and quality have obtained obvious lifting.
Liquid complex enzyme of the present invention also can be applicable in the jujube juice production of other any regional red date raw material, and other red date intensive processing field, for example in the process for processing of red date wine, red date vinegar.
Claims (7)
1. a prozyme, is characterized in that, described prozyme is comprised of acid pectase, acidic cellulase, acidic xylanase, arabanase and beta-glucosidase.
2. prozyme as claimed in claim 1, is characterized in that, described prozyme is liquid dosage form, and in prozyme, the enzyme of each component content alive is respectively: acid pectase 10000～30000u/ml; Acidic cellulase 7000～10000u/ml; Acidic xylanase 40000～60000u/ml; Arabanase 5～200u/ml; Beta-glucosidase 0.5～100u/ml.
3. prozyme as claimed in claim 2, is characterized in that, in described prozyme, each component and enzyme work are respectively: acid pectase 30000u/ml; Acidic cellulase 10000u/ml; Acidic xylanase 60000u/ml; Arabanase 5u～200u/ml; Beta-glucosidase 0.5～100u/ml.
4. the application of prozyme claimed in claim 1 in the preparation jujube juice.
5. the preparation method of a jujube juice comprises following step:
1) with red date with warm water soaking after, reheat softeningly, then making beating obtains red date slurry;
2) addition of pressing the dried jujube of 0.1-2L/ ton adds prozyme claimed in claim 1 to the red date slurry, stirs, and carries out Enzymolysis under 40-55 ℃ of constant temperature, extraction time 45-150min;
3) red date after enzymolysis slurry is sieved, centrifugal, ultrafiltration, obtain clarifying jujube juice;
4) clarifying jujube juice is concentrated, obtaining soluble solid is the concentrated clear juice of red date of 65-70 ° of Brix;
5) with the concentrated clear juice sterilization of red date, carry out sterile filling, obtain jujube juice.
6. preparation method as claimed in claim 5 is characterized in that warm water soaking in described step 1) is the warm water soaking 24h with 35 ℃ of left and right; Thermoplastic is thermoplastic 15 minutes under 70 ℃ of constant temperatures.
7. a jujube juice, is characterized in that, described jujube juice prepares with method claimed in claim 5.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106721717A (en) * | 2016-12-12 | 2017-05-31 | 山东大学 | A kind of preparation technology containing highly-water-soluble dietary fiber jujube juice or red date concentrated clear juice |
| CN110140958A (en) * | 2019-04-19 | 2019-08-20 | 浙江清荣生物科技发展有限公司 | A kind of astaxanthin oral solution and preparation method thereof |
| CN111621393A (en) * | 2020-06-02 | 2020-09-04 | 贵州弘丰果茶农业科技有限公司 | Red date fruit wine beverage and preparation method thereof |
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| CN1785065A (en) * | 2005-08-12 | 2006-06-14 | 天津市林业果树研究所 | Juice of raspberry and its processing method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106721717A (en) * | 2016-12-12 | 2017-05-31 | 山东大学 | A kind of preparation technology containing highly-water-soluble dietary fiber jujube juice or red date concentrated clear juice |
| CN106721717B (en) * | 2016-12-12 | 2020-08-07 | 山东大学 | A preparation method of fructus Jujubae juice or fructus Jujubae concentrated clear juice containing high water soluble dietary fiber |
| CN110140958A (en) * | 2019-04-19 | 2019-08-20 | 浙江清荣生物科技发展有限公司 | A kind of astaxanthin oral solution and preparation method thereof |
| CN111621393A (en) * | 2020-06-02 | 2020-09-04 | 贵州弘丰果茶农业科技有限公司 | Red date fruit wine beverage and preparation method thereof |
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