CN103156193A - Coenzyme Q10 soft capsules and preparation method thereof - Google Patents

Abstract

The invention relates to coenzyme Q10 soft capsules and a preparation method thereof. The contents of the coenzyme Q10 soft capsules comprise the following raw materials in percentage by weight: 1-20 of coenzyme Q10, 0.1-10 of vitamin E, 70-95 of corn oil and 1-20 of bee wax. The coenzyme Q10 soft capsules are prepared through the steps of manufacturing capsule bodies, preparing the content and capsules and the like. Compared with the prior art, the soft capsule preparation, which is prepared by taking the coenzyme Q10 as a main functional component and the natural vitamin E, the corn oil and the bee wax as auxiliary components, has the advantages of having the healthcare function of immunity enhancement and stable quality, being convenient to take, being in line with the requirements of State Food and Drug Administration on healthcare food, being safe and effective and being capable of being taken for a long time and is ideal healthcare food for crowd with hypoimmunity.

Description

A kind of ubiquinone 10Soft capsule and preparation method thereof

Technical field

The invention belongs to the health product technical field, especially relate to a kind of ubiquinone ₁₀Soft capsule and preparation method thereof.

Background technology

Immunity refers to body immune system identification self and dissident's material, and gets rid of the antigenicity foreign matter by immune response, to keep the function of organism physiology balance.It is the defense mechanism of a kind of human body self, is human body identification and any foreign matter (virus, bacterium etc.) of eliminating external intrusion; Process the ability of self cell and identification and processing vivo mutations cell and the virus infected cell of old and feeble, damage, dead, sex change.

Immunity can be divided into congenital immunity and acquired immunity by the difference of its acquisition pattern.When a variety of causes made immune system normally not bring into play protective effect, human body just was vulnerable to the invasion and attack of disease.Due to reasons such as now general operating pressure are large, and life style is unhealthy, people often present a kind of sub-health state, and this just easily causes the immunity function of body to descend.

The lipid peroxidation of free radical, singlet oxygen etc. is considered to cause the major reason of hypoimmunity.Peroxidation is damaged cell membrane and organelle film, image normal cell function, inducing cell generation autoimmune response finally causes the out of control of Effects on local immunological functions, causes a series of body diseases.

Ubiquinone ₁₀Be a kind of chemical constituent that extensively is present in biological cell, have no side effect.It is the basis that the human body cell energy is made, and has the metabolic cardiotonic, definite effect.Ubiquinone ₁₀Be mainly used in the treatment of acute and chronic hepatitis, acute hepatic necrosis, angiocardiopathy and hypertension are had extraordinary curative effect, it can also be used for the complex treatment of cancer, simultaneously in unique effects in aspect such as anti-ageing, antifatigue, fat-reducing and beauty treatments.Ubiquinone ₁₀Be yellow to orange-yellow crystalline powder under room temperature, fusing point lower (49-52 ℃), odorless, tasteless is dissolved in chloroform, benzene, acetone, ether or benzinum, and soluble,very slightly in ethanol is insoluble in water and methyl alcohol.Be not easy to absorb in human body, bioavilability is very poor; Ubiquinone ₁₀To light sensitive, to meet light and resolve into red material, temperature and humidity is less on its impact.

Ubiquinone ₁₀It is a kind of fat-soluble quinones, participate in the production process of Mitochondria and ATP, regulating cell redox reaction environment, the assisted Reduction electronics wear membrane process, participate in the formation of cytoplasma membrane and interior intermembranous proton gradient, it is the activator of cellular respiration and metabolism, be also important antioxidant and nonspecific immunity strengthening agent, the aspects such as recovery after the generation of organism energy, the defence of deterioration disease, immune adjusting, heart and musculature are impaired play a very important role.

Soft capsule is oiliness functional materials, the optimum formulation of health food.Because the oily functional materials can save the technical finesses such as absorption, curing, and can effectively avoid the oily functional materials to ooze out from absorb auxiliary material.In addition, functional materials and health food, trace active functional materials and the health food of the poor hydrophobic function material of low melting point functional materials and health food, bioavilability and health food, bad bitter taste and stink and functional materials and the health food of meeting light, wet, thermally labile and easy oxidation all are fit to make soft capsule.

Summary of the invention

Purpose of the present invention is exactly to provide a kind of ubiquinone that strengthens immunity function for the defective that overcomes above-mentioned prior art existence ₁₀Soft capsule and preparation method thereof.

Purpose of the present invention can be achieved through the following technical solutions:

A kind of ubiquinone ₁₀Soft capsule comprises the content in utricule and inclosure utricule, and described content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀1～20, vitamin E 0.1～10, corn oil 70～95, beeswax 1～20.

As preferably, described content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀4～6, vitamin E 3～5, corn oil 84～g7, beeswax 3～6.

As most preferably, described content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀4.8, vitamin E 3.7, corn oil 86, beeswax 5.5.

Described vitamin E is the natural VE of 1000IU/g.

A kind of ubiquinone ₁₀The preparation method of soft capsule, the method comprises the following steps:

(1) make utricule: take gelatin, glycerine and pure water as the utricule raw material, glycerine and pure water are injected in glue pot, be heated with stirring to 60～70 ℃, then add gelatin, continue to stir final vacuum deaeration in 20～30 minutes, make obtaining utricule;

(2) preparation content: after corn oil, beeswax are heated to 60～75 ℃ of melting mixings, are cooled to 40～50 ℃, and add ubiquinone by formula ratio ₁₀And natural VE, stirred 20～30 minutes, make suspension, suspension slowly passes through colloid mill 2～3 times, after the vacuum outgas bubble, obtains content;

(3) preparation capsule: content and step (1) making of step (2) preparation are obtained utricule process pelleting, finalize the design, wash ball, drying, choose ball and packing step, prepare ubiquinone ₁₀Soft capsule.

In step (1), the weight ratio of gelatin, glycerine and pure water is 5～20: 1～10: 5～20.

Ubiquinone (coenzyme Q), being called for short CoQ, is the fat-soluble quinones that extensively exists in organism, plays an important role in proton and electronic transfer process in respiratory chain in vivo, being the activator of cellular respiration and metabolism, is also important antioxidant and nonspecific immunity strengthening agent.The number of the ubiquinone side chain iso-amylene unit of separate sources is different, and in the mankind and other mammiferous ubiquinones, isopentene group unit's number is 10, therefore be referred to as ubiquinone ₁₀, i.e. primary raw material in the present invention formula.

Ubiquinone ₁₀The chemical name CoQ10, molecular formula C ₅₉H ₉₀O ₄, molecular weight 862, structure is similar to vitamin K, belongs to quinone lopps compound.At room temperature be orange-yellow crystal, odorless, tasteless.Its fusing point is 49 ℃, affected by temperature and humidity less, but unstable under illumination condition, easily is decomposed.

Ubiquinone ₁₀In the quinone ring of structure, benzoquinones has hydroxyl substituent and makes it tend to polarity, and the polyisoprene side chain makes it have lower free energy in hydrophobic environment, and this specific character can spread rapidly in mitochondrial inner membrane and enrichment.In addition, its quinone ring has typical invertibity redox characteristic.Exist with the quinone form under oxidizing condition, under oxygen free condition, reducible is hydroquinones, has the form of oxidized form, reduced form and free radical semiquinone in cell.Based on above reason, ubiquinone ₁₀Accept hydrogen from Mitochondria complex after, directly transmit on the one hand hydrogen to free radical, react under the antioxidase effect, suppress the destruction of radical pair cell membrane, on the other hand with proton release to mitochondrial matrix, electronics passes to cytochromes, accelerating oxidation phosphorylation and electronics initiatively shift, and form thus the main matter ATP that organism ability is stored, and lose to improve the ATP level by reducing AMP, reduce calcium ion and run off, keep calcium channel integrality and stabilizing cell membrane.In actual applications, ubiquinone ₁₀Be considered to a kind of natural good antioxidant and free radical scavenger, the performance alleviating physical fatigue improves the multiple efficacies effects such as immunity.So ubiquinone ₁₀Be a kind of immunomodulator, performance strengthens the health care of immunity.

In addition, ubiquinone ₁₀Large, the poorly water-soluble of molecule, gastrointestinal absorption is slow, the time that arrives valid density after oral administration is longer, approximately need 5～10 hours (average 6.5 hours), so the present invention selects the formulation of soft capsule.

Vitamin E is a kind of liposoluble vitamin that extensively is present in natural animal-plant, claims again tocopherol, to acid and thermally-stabilised, when having oxygen to exist, is heated to 200 ℃ and still can keeps stable.Vitamin E has different physiological roles, is also that one of needed by human vitamin is one of topmost antioxidant.

Vitamin E is positioned on cell membrane; belong to fat-soluble chain rupture type antioxidant; it provides hydrogen ion to the fat oxygen radical; make the lipid peroxidation chain interruption; antioxidant system together with superoxide dismutase, glutathione peroxidase in constituting body, Cell protection film and intracellular nucleic acid are avoided the attack of free radical.In addition, the vitamin E Hai Neng temper singlet oxygen that goes out improves the oxidation resistance of solvent naphtha.In formula of the present invention, add vitamin E as auxiliary material, can effectively protect ubiquinone ₁₀The oxidation inactivation.

Natural VE is by extracting refining forming in natural plants, and is higher than the synthesising complex E security, and natural VE absorbs high 3.5 times than composite, and the half-life is longer, and physiologically active is approximately 3-8 times of composite.Thereby the present invention selects natural VE as the major auxiliary burden that adds.

Due to ubiquinone ₁₀Be liposoluble substance, be fit to use oil as soft capsule matrix, corn oil is a kind of high-quality edible vegetable oil, and it forms take maize germ as feed purification.Due to the corn oil stable in properties, high temperature resistant, be usually used in the production of medicine and health food, therefore selecting it is auxiliary material of the present invention.

Due to ubiquinone ₁₀Can not dissolve fully in corn oil, the slow sedimentation layering of meeting in production and old storage process, the suspension that therefore need add therein the suspending agent preparation to have good stability, guarantee the suspension ability of product, prevent soft capsule before capsule, in the capsule process and the sedimentation of solids in suspension after capsule, guarantee loading amount accurately.Oiliness suspending agent commonly used has wax lipid, lecithin, aluminum monostearate etc., and wherein beeswax by affecting system viscosity and rheological property, changes every stability indicator.Lecithin plays a role to redispersibility and change of size as surfactant.Aluminum monostearate is a kind of thixotrope, forms in vegetable oil when consumption 2% and stablizes the thixotroping system.But consider the economic, practical and safety of raw material, the use of beeswax is the most extensive.Beeswax is the wax of honeybee secretion, a kind of organic mixture that honeycomb makes after eliminating or centrifugal removal honey.Concentration of beeswax is larger, and plastic yield is larger, begins to occur the more prolongation of time of sedimentation, and sinking speed is less, meets the stokes formula.But concentration of beeswax is excessive, can cause the product poor fluidity, and the main component of beeswax is palmitic acid beeswax alcohol ester, contains simultaneously a small amount of free polyols, thereby has certain emulsibility, reduces surface tension, and this also can make the sinking speed of suspension slow down.

Compared with prior art, the present invention is with ubiquinone ₁₀Be main effect composition, be aided with natural VE, corn oil and beeswax and make soft capsule preparation, has the health care that strengthens immunity, constant product quality, taking convenience, meet State Food and Drug Administration to the requirement of health food, safe and effective, but long-term taking is the desirable health food of hypoimmunity crowd.

The specific embodiment

The present invention is described in detail below in conjunction with specific embodiment.

Embodiment 1

A kind of ubiquinone ₁₀Soft capsule comprises the content in utricule and inclosure utricule, and content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀4.8, vitamin E 3.7, corn oil 86, beeswax 5.5.Wherein, vitamin E is the natural VE of 1000IU/g.

A kind of ubiquinone ₁₀The preparation method of soft capsule, the method comprises the following steps:

(1) make utricule: take gelatin, glycerine and pure water as the utricule raw material, glycerine and pure water are injected in glue pot, be heated with stirring to 65 ℃, add again gelatin, continue to stir final vacuum deaeration in 25 minutes, make obtaining utricule, wherein, the weight ratio of gelatin, glycerine and pure water is 5: 10: 5;

(2) preparation content: after corn oil, beeswax are heated to 70 ℃ of melting mixings, are cooled to 45 ℃, and add ubiquinone by formula ratio ₁₀And natural VE, stirred 25 minutes, make suspension, suspension slowly passes through colloid mill 2 times, after the vacuum outgas bubble, obtains content;

(3) preparation capsule: content and step (1) making of step (2) preparation are obtained utricule process pelleting, finalize the design, wash ball, drying, choose ball and packing step, prepare ubiquinone ₁₀Soft capsule.

Ubiquinone to the present embodiment preparation ₁₀The enhancing immunity function of soft capsule detects.

1, sample: the ubiquinone of the present embodiment preparation ₁₀Soft capsule.

2, experimental animal and environment: SPF Kunming kind small white mouse, credit number: SCXK (Shanghai) 2002-0010, body weight 18～22 grams, female 100, male 100, be divided into four immunity and organize greatly, 50 of every large groups, the mouse of the large group of each immunity is divided into water control group, solvent control group, basic, normal, high dosage group, each 10.Immunity one group (50 of female mices) is used for delayed allergy (DTH) experiment, the mensuration of serum hemolysin, the mensuration of antibody-producting cell number; Immunity two groups (50 of male mices) is used for mouse carbon and cleans up experiment; Immunity three groups (50 of female mices) is used for Turnover of Mouse Peritoneal Macrophages and engulfs the chicken red blood cell experiment; Immunity four groups (50 of male mices) is used for mouse lymphocyte transformation experiment and NK cells in mice activity experiment mensuration.22 ± 2 ℃ of experimental situation temperature, relative humidity 50% ± 10%.

3, the dosage design gives mode with tested material: ubiquinone of the present invention ₁₀Soft capsule is 1.0g/60kg body weight every day to the RD of human body, be equivalent to 0.0167g/kg.BW, determine high Three doses in hanging down of mouse by 5,10,30 multiple doses that are equivalent to the human body RD, dosage to mouse in the present embodiment is made as respectively 0.083g/kg.BW, 0.167g/kg.BW, 0.50g/kg.BW three kinds, per os gavage once a day gives the mouse tested material, the gavage volume is pressed 10ml/kg.BW.Prepare tested material with corn oil before gavage, in basic, normal, high dosage group, the concentration of tested material is respectively 8.33g/L, 16.667g/L and 50.00g/L, negative control group replaces tested material with isopyknic distilled water, the solvent control group replaces tested material with isopyknic corn oil, tests every immune indexes after 30 days.Mouse feeds with outbreeding system mouse special feed and raises.

4, experimental technique

4.1 organ weight ratio pH-value determination pH

The mouse rear cervical vertebra dislocation of weighing is put to death, and gets its thymus gland, spleen, removes most manadesma, blots the organ surface blood stains and weighs with filter paper, calculates thymus gland body weight ratio and spleen body weight ratio.The data obtained carries out variance analysis with the PEMS statistical software.

4.2 delayed allergy (the sufficient sole of the foot thickens method) (DTH)

Get sheep blood, with physiological saline washing three times, every mouse is through lumbar injection 2% (v/v, configure with physiological saline) hematocrit SRBC (2000r/min, 10min) 0.2ml after sensitization 4 days, measures the sufficient sole of the foot thickness in left and right, same position is measured 3 times, average, then at measuring point hypodermic injection 20% (v/v configures with physiological saline) hematocrit SRBC20 μ l, inject and measured the sufficient sole of the foot thickness in left and right in rear 24 hours, represent the degree of DTH with the difference of sufficient sole of the foot thickness before and after attacking.The data obtained is measurement data, carry out variance analysis with the SPSS statistical software, if before and after tested material is attacked, the difference of sufficient sole of the foot thickness is higher than control group, and difference has conspicuousness (P＜0.05), can conclude that this tested material is improved the effect of mouse delayed allergy ability.

4.3ConA the mouse spleen lymphocyte conversion test (mtt assay) of inducing

Animal gives sample after 30 days continuously, and the cervical vertebra dislocation is put to death, and gets spleen, makes splenocyte suspension, and adjusting cell concentration is 3 * 10 ⁶Individual/ml is divided into two holes with cell suspension and adds 24 well culture plates Chinese, every hole 1ml, and a hole adds 75 μ lConA liquid (being equivalent to 7.5 μ g/ml), and 5%CO is put in contrast in another hole ₂, cultivate 68h for 37 ℃.Cultivate and finish front 4h, every hole gentle aspiration supernatant 0.7ml, add 0.7ml not contain the PRMI1640 nutrient solution of calf serum, add simultaneously MTT (5mg/ml) 50 μ l/ holes, continue to cultivate 4h, after cultivating end, every hole adds 1ml acid isopropyl alcohol, the piping and druming mixing after purple crystal is dissolved fully, then moves into liquid in 96 orifice plates, every hole adds 3 Duplicate Samples, measure absorbance (A value) with ELIASA at the 570nm wavelength, calculate the poor of test hole A value and control wells A value, to represent lymphocytic competence for added value.Result is analyzed with variance analysis.If tested material group Proliferation of lymphocytes ability is higher than control group, and difference has conspicuousness, can judge that this tested material is improved the effect of the mouse spleen lymphocyte conversion capability that ConA induces.

4.4 antibody-producting cell is measured (Jerne improves slide method)

Animal gives sample after 30 days continuously, and after 5 days, the dislocation of animal cervical vertebra is put to death, and takes out spleen, makes splenocyte suspension with defiber Mianyang erythrocyte immune, and adjusting cell concentration is 5 * 10 ⁶Individual/ml.After top layer culture medium (the 1g agarose adds distilled water 100ml) heating for dissolving, put into 45 ℃ of water bath heat preservations, mix packing small test tube, every pipe 0.5ml with the Hanks liquid of equivalent pH7.2-7.4,2 times of concentration, add 50 μ l10%SRBC again in pipe, 20 μ l splenocyte suspensions, mixing, be poured on the 6cm plate of brushing thin agar layer rapidly, after continuing incubation 1.5h, counting hemolysis plaque number.Result is added up with variance analysis.If tested material group Proliferation of lymphocytes ability is higher than control group, and difference has conspicuousness, can judge that this tested material has the effect that strengthens the mouse antibodies cell number.

4.5 mice serum hemolysin test:

get sheep blood, with physiological saline washing 3 times, every mouse (configures with physiological saline through lumbar injection 2%, volume ratio) hematocrit SRBC (2000r/min, 10min) 0.2ml, after immunity 5 days, extract eyeball of mouse and get blood in centrifuge tube, placed approximately 1 hour, solidification blood and tube wall are peeled off, centrifugal, collect serum, with physiological saline with the serum doubling dilution, different dilution factor serum are placed in respectively in the solidifying brassboard of trace snow, every hole 100 μ l, add again 0.5%SRBC suspension 100 μ l, mixing, put in wet box, 37 ℃ of incubations 3 hours, record the hemagglutination degree, calculating antibody product (serum two-fold dilution index and the aggegation degree sum of products).

The serum agglutination degree is divided into 5 grades, clicks standard determination.0 grade: SRBC all sinks, and concentrates on and forms fine and close round point shape, surrounding liquid clear at the bottom of the hole; The I level: the SRBC major part is deposited on the bottom, hole and becomes round point shape, and surrounding has the SRBC of a small amount of aggegation; The II level: the SRBC of aggegation forms thin layer at the bottom of the hole, and a loose red point can be obviously seen at the center; The III level: the SRBC of aggegation all with paving become skim at the bottom of being dispersed in the hole, a small red dot mays be seen indistinctly at the center; The IV level: the SRBC of aggegation paving uniformly becomes skim at the bottom of being dispersed in the hole, and grumeleuse becomes the convolution shape sometimes.

The data obtained carries out variance analysis with PEMS software, if tested material group Hemolysin product higher than control group, and difference has conspicuousness, can judge that tested material is improved the effect of mice serum hemolysin level.

4.6 mouse carbon clearance test:

Animal gives sample after 30 days continuously, and the india ink of tail vein injection dilution in 1: 5 treats that prepared Chinese ink injects, timing immediately.Injected after prepared Chinese ink 2,10 minutes, and got blood 20 μ l from the angular vein clump respectively, and it is added in the 2ml sodium carbonate liquor, at 600nm wavelength place's test absorbance, do blank with sodium carbonate liquor with spectrophotometer.According to the weight of animals, liver weighs and spleen re-computation phagocytic index, and result is added up with variance analysis.If tested material group Hemolysin product is higher than control group, and difference has conspicuousness, can judge that tested material is improved the effect that the carbon of mouse monokaryon-macrophage is cleaned up ability.

4.7 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell test (half intracorporal method):

Animal gives sample after 30 days continuously, every mouse lumbar injection 20% chicken erythrocyte suspension 1ml, interval 30 minutes, the cervical vertebra dislocation is put to death, and is fixed on the mouse plate, cuts off abdominal skin, injecting normal saline 2ml, rotated the mouse plate 1 minute, sucking-off abdominal cavity washing lotion 1ml divides and drips on 2 sheet glass sheets, 37 ℃ of wet incubating 30 minutes of incubator, use the physiological saline rinsing, dry, fix with 1: 1 acetone methanol solution, 4%Giemsa-phosphate buffer dyeing 3 minutes, dry with the distilled water rinsing again, with oily mirror microscopy, calculate percentage phagocytosis and phagocytic index.Wherein, the macrophage number of the macrophage number of phagocytic rate (%)=engulf chicken red blood cell/counting * 100;

The macrophage number of chicken red blood cell sum/counting that phagocytic index=quilt is engulfed.

The phagocytic rate of acquired results is pressed

Convert, in formula, P is phagocytic percentage, expression decimally, if the phagocytic rate of tested material or phagocytic index apparently higher than control group, and difference has conspicuousness, can judge that tested material is improved the effect of macrophage phagocytic chicken red blood cell ability.

4.8 NK cells in mice determination of activity:

Animal gives sample after 30 days continuously, and the cervical vertebra dislocation is put to death, and gets spleen, makes splenocyte suspension (effector cell), gets the rear 24h YAC-1 cell that goes down to posterity and adds 1640 complete culture solutions, and adjusting cell concentration is 4 * 10 ⁵individual/ml (target cell), get each 100 μ l effect target of target cell and effector cell than (50: 1), add U-shaped 96 well culture plates, target cell Spontaneous release hole adds target cell and each 100 μ l of nutrient solution, maximum release aperture adds target cell and each 100 μ l of 1%NP40, above-mentioned every three multiple holes that are equipped with, with 37 ℃, cultivated 4 hours in 5% CO2gas incubator, every hole is drawn supernatant 100 μ l and is placed in flat 96 well culture plates, add simultaneously LDH matrix liquid 100 μ l, reaction 3min, every hole adds the HCL30 μ l of 1mol/l to stop, measure the A value at the 490mm place with ELIASA.Calculate as follows the NK cytoactive:

NK cytoactive %=(reacting hole A-Spontaneous release hole A)/(maximum release aperture A-Spontaneous release hole A) * 100;

The NK cytoactive need be pressed

Carry out data transaction, in formula, P is the NK cytoactive, expression decimally, if the phagocytic rate of tested material or phagocytic index apparently higher than control group, and difference has conspicuousness, can judge that tested material is improved the effect of NK cells in mice activity.

5 experimental results

5.1 experimental result is judged

Strengthening the immunocompetence function judges: any two aspect results are positive aspect four of cellular immune functions, humoral immune function, monocytes/macrophages function, NK cytoactive, can judge that this tested material has the effect of the immunity function of enhancing.

Wherein two experimental results in the cellular immune function assay project are all positive, or two dosage group results of arbitrary experiment are positive, can judge that the cellular immune function assay result is positive.Two experimental results in humoral immune function mensuration project are all positive or two dosage group results arbitrary experiment are positive, can judge that the humoral immune function measurement result is positive.Two experimental results in monocytes/macrophages functional examination project all two dosage group results of positive or arbitrary experiment are positive, can judge that monocytes/macrophages functional examination result is positive.An above dosage group result of NK cytoactive detection experiment is positive, can judge that NK cytoactive result is positive.

5.2 ubiquinone ₁₀Soft capsule sees Table 1 to the impact of Mouse Weight

The impact of table 1 sample on an immune treated animal body weight (gram)

Different dosage is compared with control group as can be seen from Table 1, the weight gain there was no significant difference of mouse, so ubiquinone ₁₀The body weight not obviously impact of soft capsule on animal.

5.3 ubiquinone ₁₀The impact of soft capsule on mice organs body weight ratio: see Table 2, table 3.

Table 2 ubiquinone ₁₀The impact of soft capsule on mouse spleen body weight ratio

Different dosage is compared with control group as can be seen from Table 2, the spleen body weight ratio there was no significant difference of mouse, so ubiquinone ₁₀The spleen body weight ratio not obviously impact of soft capsule on animal.

Table 3 ubiquinone ₁₀The impact of soft capsule on mouse spleen body weight ratio

Different dosage is compared with control group as can be seen from Table 3, the thymus gland body weight ratio there was no significant difference of mouse, so ubiquinone ₁₀The thymus gland body weight ratio not obviously impact of soft capsule on animal.

5.4 ubiquinone ₁₀The impact of soft capsule on the mouse cell immunologic function

A, ubiquinone ₁₀Soft capsule sees Table 4 to the impact of mouse delayed allergy (DTH).

Table 4 ubiquinone ₁₀The impact of soft capsule on mouse delayed allergy (DTH)

By as seen from Table 4, the impact of the mouse swelling degree of the paw of water control group, solvent control group and 3 dosage groups is different, and middle dosage group and high dose group have notable difference on the impact of mouse swelling degree of the paw with the control group ratio, thinks ubiquinone ₁₀Soft capsule is improved the ability of mouse delayed allergy (DTH).

B, ubiquinone ₁₀The impact of the mouse spleen lymphocyte transformation experiment that soft capsule is induced ConA sees Table 5.

Table 5 ubiquinone ₁₀The impact of the mouse spleen lymphocyte transformation experiment that soft capsule is induced ConA

By as seen from Table 5, water control group, solvent control group and 3 dosage groups are to there was no significant difference between the lymphopoiesis ability of mouse, think ubiquinone ₁₀Soft capsule is on improving the not obviously impact of mouse lymphocyte multiplication capacity.

5.4 ubiquinone ₁₀The impact of soft capsule on the mouse humoral immune function

A, ubiquinone ₁₀The impact of soft capsule on mouse antibodies cellulation number sees Table 6.

Table 6 ubiquinone ₁₀The impact of soft capsule on mouse antibodies cellulation number

By as seen from Table 6, water control group and middle dosage group and high dose group have obvious difference to the impact of plaque number, think ubiquinone ₁₀Soft capsule has the ability that increases mouse antibodies cellulation number.

B, ubiquinone ₁₀The impact of soft capsule on the mice serum hemolysin level sees Table 7.

Table 7 ubiquinone ₁₀The impact of soft capsule on the mice serum hemolysin level

By as seen from Table 7, the impact of water control group and middle dosage group and high dose group antagonist product has obvious difference, thinks ubiquinone ₁₀Soft capsule has the ability that improves the mice serum hemolysin level.

5.5 ubiquinone ₁₀The impact of soft capsule on mouse monokaryon-macrophage phagocytic function

A, ubiquinone ₁₀Soft capsule is cleaned up the impact of ability, is seen Table 8 mouse monokaryon-macrophage carbon.

Table 8 ubiquinone ₁₀Soft capsule is cleaned up the impact of ability to mouse monokaryon-macrophage carbon

By as seen from Table 8, water control group, solvent control group and 3 dosage groups are cleaned up there was no significant difference between ability to the monocytes/macrophages carbon of mouse, think ubiquinone ₁₀Soft capsule is cleaned up not obviously impact of ability to mouse carbon.

B, ubiquinone ₁₀Soft capsule is engulfed the impact of chicken red blood cell ability on Turnover of Mouse Peritoneal Macrophages, see Table 9, table 10.

Table 9 ubiquinone ₁₀The impact of soft capsule on Turnover of Mouse Peritoneal Macrophages chicken red blood cell phagocytic rate

Table 10 ubiquinone ₁₀The impact of soft capsule on Turnover of Mouse Peritoneal Macrophages chicken red blood cell phagocytic index

By table 9 and as seen from Table 10, water control group, solvent control group and 3 dosage groups are engulfed there was no significant difference between the chicken red blood cell ability to Turnover of Mouse Peritoneal Macrophages, think ubiquinone ₁₀Soft capsule is engulfed the not obviously impact of chicken red blood cell ability to Turnover of Mouse Peritoneal Macrophages.

5.6 ubiquinone ₁₀The impact of soft capsule on the NK cells in mice activity sees Table 11.

Table 11 ubiquinone ₁₀The impact of soft capsule on the NK cells in mice activity

By as seen from Table 11, water control group, solvent control group and 3 dosage groups are to there was no significant difference between the NK cells in mice activity, think ubiquinone ₁₀Soft capsule is on the active not obviously impact of NK cells in mice.

To sum up, per os gives the ubiquinone of mouse various dose group ₁₀Soft capsule 30 days, weight of mice is had no adverse effects, and without impact, the humoral immune function of mouse, cellular immune function assay result are all positive on mouse spleen and thymus gland body weight ratio, monocytes/macrophages phagocytic activity, NK cytoactive detection result are all negative, show ubiquinone ₁₀Soft capsule has the effect that strengthens immunity function.

Embodiment 2

A kind of ubiquinone ₁₀Soft capsule comprises the content in utricule and inclosure utricule, and content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀1, vitamin E 3, corn oil 95, beeswax 1.Wherein, vitamin E is the natural VE of 1000IU/g.

A kind of ubiquinone ₁₀The preparation method of soft capsule, the method comprises the following steps:

(1) make utricule: take gelatin, glycerine and pure water as the utricule raw material, glycerine and pure water are injected in glue pot, be heated with stirring to 60 ℃, add again gelatin, continue to stir final vacuum deaeration in 30 minutes, make obtaining utricule, wherein, the weight ratio of gelatin, glycerine and pure water is 5: 1: 5;

(2) preparation content: after corn oil, beeswax are heated to 60 ℃ of melting mixings, are cooled to 40 ℃, and add ubiquinone by formula ratio ₁₀And natural VE, stirred 20 minutes, make suspension, suspension slowly passes through colloid mill 2 times, after the vacuum outgas bubble, obtains content;

(3) preparation capsule: content and step (1) making of step (2) preparation are obtained utricule process pelleting, finalize the design, wash ball, drying, choose ball and packing step, prepare ubiquinone ₁₀Soft capsule.

Embodiment 3

A kind of ubiquinone ₁₀Soft capsule comprises the content in utricule and inclosure utricule, and content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀20, vitamin E .0.1, corn oil 70, beeswax 9.9.Wherein, vitamin E is the natural VE of 1000IU/g.

A kind of ubiquinone ₁₀The preparation method of soft capsule, the method comprises the following steps:

(1) make utricule: take gelatin, glycerine and pure water as the utricule raw material, glycerine and pure water are injected in glue pot, be heated with stirring to 70 ℃, add again gelatin, continue to stir final vacuum deaeration in 20 minutes, make obtaining utricule, wherein, the weight ratio of gelatin, glycerine and pure water is 20: 10: 20;

(2) preparation content: after corn oil, beeswax are heated to 75 ℃ of melting mixings, are cooled to 50 ℃, and add ubiquinone by formula ratio ₁₀And natural VE, stirred 30 minutes, make suspension, suspension slowly passes through colloid mill 3 times, after the vacuum outgas bubble, obtains content;

(3) preparation capsule: content and step (1) making of step (2) preparation are obtained utricule process pelleting, finalize the design, wash ball, drying, choose ball and packing step, prepare ubiquinone ₁₀Soft capsule.

Embodiment 4

A kind of ubiquinone ₁₀Soft capsule comprises the content in utricule and inclosure utricule, and content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀3, vitamin E2, corn oil 75, beeswax 20.Wherein, vitamin E is the natural VE of 1000IU/g.

A kind of ubiquinone ₁₀The preparation method of soft capsule, the method comprises the following steps;

(1) make utricule: take gelatin, glycerine and pure water as the utricule raw material, glycerine and pure water are injected in glue pot, be heated with stirring to 65 ℃, add again gelatin, continue to stir final vacuum deaeration in 25 minutes, make obtaining utricule, wherein, the weight ratio of gelatin, glycerine and pure water is 10: 3: 10;

(2) preparation content: after corn oil, beeswax are heated to 65 ℃ of melting mixings, are cooled to 45 ℃, and add ubiquinone by formula ratio ₁₀And natural VE, stirred 25 minutes, make suspension, suspension slowly passes through colloid mill 2 times, after the vacuum outgas bubble, obtains content;

(3) preparation capsule: content and step (1) making of step (2) preparation are obtained utricule process pelleting, finalize the design, wash ball, drying, choose ball and packing step, prepare ubiquinone ₁₀Soft capsule.

Embodiment 5

Difference from Example 4 is: content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀5, vitamin e1 0, corn oil 75, beeswax 10.

Embodiment 6

Difference from Example 4 is: content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀6, vitamin E 4, corn oil 84, beeswax 6.

Embodiment 7

Difference from Example 4 is: content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀4, vitamin E 3, corn oil 87, beeswax 6.

Embodiment 8

Difference from Example 4 is: content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀6, vitamin E 5, corn oil 86, beeswax 3.

Claims (6)

1. ubiquinone ₁₀Soft capsule comprises the content in utricule and inclosure utricule, it is characterized in that, described content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀1～20, vitamin E 0.1～10, corn oil 70～95, beeswax 1～20.

2. a kind of ubiquinone according to claim 1 ₁₀Soft capsule is characterized in that, described content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀4～6, vitamin E 3～5, corn oil 84～87, beeswax 3～6.

3. a kind of ubiquinone according to claim 1 ₁₀Soft capsule is characterized in that, described content is comprised of the raw material of following component and percentage by weight: ubiquinone ₁₀4.8, vitamin E 3.7, corn oil 86, beeswax 5.5.

4. according to claim 1～3 arbitrary described a kind of ubiquinones ₁₀Soft capsule is characterized in that, described vitamin E is the natural VE of 1000IU/g.

5. described ubiquinone as arbitrary in claim 1～3 ₁₀The preparation method of soft capsule is characterized in that, the method comprises the following steps:

(1) make utricule: take gelatin, glycerine and pure water as the utricule raw material, glycerine and pure water are injected in glue pot, be heated with stirring to 60～70 ℃, then add gelatin, continue to stir final vacuum deaeration in 20～30 minutes, make obtaining utricule;

(2) preparation content: after corn oil, beeswax are heated to 60～75 ℃ of melting mixings, are cooled to 40～50 ℃, and add ubiquinone by formula ratio ₁₀And natural VE, stirred 20～30 minutes, make suspension, suspension slowly passes through colloid mill 2～3 times, after the vacuum outgas bubble, obtains content;

(3) preparation capsule: content and step (1) making of step (2) preparation are obtained utricule process pelleting, finalize the design, wash ball, drying, choose ball and packing step, prepare ubiquinone ₁₀Soft capsule.

6. a kind of ubiquinone according to claim 5 ₁₀The preparation method of soft capsule is characterized in that, in step (1), the weight ratio of gelatin, glycerine and pure water is 5～20: 1～10: 5～20.