CN103141433A - Construction method for livestock animal model with critical lack of Vitamin A - Google Patents
Construction method for livestock animal model with critical lack of Vitamin A Download PDFInfo
- Publication number
- CN103141433A CN103141433A CN2013100731958A CN201310073195A CN103141433A CN 103141433 A CN103141433 A CN 103141433A CN 2013100731958 A CN2013100731958 A CN 2013100731958A CN 201310073195 A CN201310073195 A CN 201310073195A CN 103141433 A CN103141433 A CN 103141433A
- Authority
- CN
- China
- Prior art keywords
- vitamin
- animal model
- poultry
- purifying
- level
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention relates to a construction method for a livestock animal model with a critical lack of Vitamin A. Breeding livestock animals in the egg-laying period are fed with purified ration lacking Vitamin A, so as to reduce the level of serum VA to 0.60-0.85 mu mol/L, and the level is kept through the Vitamin A solution drinking water, and the produced breeding eggs are hatched, so that a one-day-aged livestock animal model with a critical lack of Vitamin A is obtained. By applying the method, the established animal model has the feature of critical lack of Vitamin A, can survive to more than 5-week-age due to physical strength, and has the characteristics of high uniformity within groups, stable performance, and convenience for commercial preparation.
Description
Construction?of?model?poultry?with?marginal?vitamin?A?deficiency
Technical field
The invention belongs to Animal nutrition related scientific research, veterinary clinic evaluation and experimental zoology field, relate to the construction method of deficiency disease animal model, especially relate to the construction method of the critical shortage animal model of a kind of poultry vitamin A.
Background technology
In recent years, along with poultry nutrition and immunologic development, the nutrition immune regulating action of vitamin A day by day is subject to people and payes attention to.Vitamin A all has important regulating action by its metabolite retinoic acid in animal body at the aspects such as respiratory mucosa, alimentary canal mucous membrane, humoral immunity and cellular immunity of poultry.Yet in the nutrition immune adjusting and research mechanism of action aspect thereof of research at vitamin A, the animal model that builds the critical shortage of poultry vitamin A becomes bottleneck.Although the purifying daily ration of the existing A that is deficient in vitamin by feeding builds the report of animal model at present, but also mainly there is following problem in its technological means: 1. because poultry liver stores many vitamin As, often need the to feed purifying daily ration of the A that is deficient in vitamin of a very long time just can show the critical shortage of vitamin A.2. because the structure time is longer, and poultry growth cycle short (for example commercial meat bird only need about 40 days deliver for sale), carries out early stage test (as 1-2 age in week) and be difficult to realize.3. because chicken is very large to the variation of the links such as the absorption of usual vitamin A, transhipment, often in the same colony that processes, the trophic level of Different Individual vitamin A is uneven.4. directly adopt the purifying daily ration of the A that is deficient in vitamin to build model, cause that easily animal vitamin A severe lacks and loses appetite, and then cause that albumen-energy nutrient imbalance causes death.
Summary of the invention
In view of the deficiencies in the prior art, the object of the present invention is to provide a kind of young fowl animal model of 1 age in days that builds the critical shortage of vitamin A.The critical shortage animal model of vitamin A that builds by this method, has in colony regularity high, the characteristics such as stable performance, the therapeutic evaluation, vitamin A nutrition immune that can satisfy veterinary clinic vitamin A replenishers are regulated and the needs of the scientific research of mechanism, other correlative studys of experimental zoology etc.
In order to solve the existing problem of background technology, the present invention by the following technical solutions:
Prepare the purifying daily ration of the A that is deficient in vitamin, the raw material that comprises following ratio of weight and number: casein 15.0-25.0, DL-METHIONINE 1.5-4.5, corn starch 10.0-18.0, sucrose 40.0-60.0, cellulose 4.0-6.0, corn oil 4.0-6.0, microelement pre-mix material 3.5, vitamin premix 1.0.Wherein, microelement pre-mix material is pressed the poultry nutrition standard preparation that U.S. NRC recommends announcement, and vitamin premix is not except containing vitamin A, and all the other compositions are all prepared by the poultry nutrition standard that U.S. NRC recommends.Prepare in addition the vitamin A microemulsion solution, comprise the raw material of following ratio of weight and number: oleovitamin A (content 1000000IU/g) 0.1, propane diols 10.0-15.0, Span60 10.0-15.0, Tween80 8.0-12.0, BHT 0.3, sodium pyrosulfite 0.3, purified water 60-70 part.Can adjust flexibly poultry vitamin A intake by vitamin A solution drinking-water.Select the father and mother in 18-25 age in week for kind of a fowl, the purifying daily ration of A of being deficient in vitamin of feeding is measured Serum Vitamin A content at interval of 1 all wing venous blood collections.After kind of a fowl serum vitamin A level is reduced to 0.60-0.85 μ mol/L, adjusts its absorption vitamin a level by drinking-water and be stabilized in the 600IU/kg daily ration.In this scope, do not affect its laying rate and incubation rate, plant the critical deficiency state that young fowl that egg hatches namely reaches vitamin A, and don't as for due to the too weak Deaths that occurs of physique.Go out the young fowl of the shell purifying age in days of A of being deficient in vitamin of feeding, coordinate vitamin A microemulsion solution drinking-water, adjusting it, to take in vitamin A daily ration level be 300IU/kg, can keep the critical deficiency state of vitamin A of young fowl.
Preferably, prepare the purifying daily ration of the A that is deficient in vitamin, the raw material that comprises following ratio of weight and number: casein 18.0-22.0, DL-METHIONINE 1.7-4.2, corn starch 12.0-16.0, sucrose 42.0-55.0, cellulose 4.5-5.5, corn oil 4.5-5.5, microelement pre-mix material 3.5, vitamin premix 1.0.Wherein, microelement pre-mix material is pressed the poultry nutrition standard preparation that U.S. NRC recommends announcement, and vitamin premix is not except containing vitamin A, and all the other compositions are all prepared by the poultry nutrition standard that U.S. NRC recommends.Prepare in addition the vitamin A microemulsion solution, comprise the raw material of following ratio of weight and number: oleovitamin A (content 1000000IU/g) 0.1, propane diols 10.0-12.0, Span60 12.0-15.0, Tween80 8.0-10.0, BHT 0.3, sodium pyrosulfite 0.3, purified water 60-65 part.Select the father and mother in 19-22 age in week for kind of a fowl, the purifying daily ration of A of being deficient in vitamin of feeding is measured Serum Vitamin A content at interval of 1 all wing venous blood collections.After kind of a fowl serum vitamin A level is reduced to 0.60-0.85 μ mol/L, adjusts its absorption vitamin a level by drinking-water and be stabilized in the 600IU/kg daily ration.In this scope, do not affect its laying rate and incubation rate, plant the critical deficiency state that young fowl that egg hatches namely reaches vitamin A, and don't as for due to the too weak Deaths that occurs of physique.Go out the young fowl of the shell purifying age in days of A of being deficient in vitamin of feeding, coordinate the agent of vitamin A micro emulsion drinking water, adjusting it, to take in vitamin A daily ration level be 120IU/kg, can keep the critical deficiency state of vitamin A of young fowl.
Further preferably, prepare the purifying daily ration of the A that is deficient in vitamin, the raw material that comprises following ratio of weight and number: casein 19.0-20.0, DL-METHIONINE 1.9-4.0, corn starch 14.0-15.0, sucrose 44.0-52.0, cellulose 4.7-5.2, corn oil 4.7-5.2, microelement pre-mix material 3.5, vitamin premix 1.0.Wherein, microelement pre-mix material is pressed the poultry nutrition standard preparation that U.S. NRC recommends announcement, and vitamin premix is not except containing vitamin A, and all the other compositions are all prepared by the poultry nutrition standard that U.S. NRC recommends.Prepare in addition the vitamin A microemulsion solution, comprise the raw material of following ratio of weight and number: oleovitamin A (content 1000000IU/g) 0.1, propane diols 10.0-11.0, Span60 12.0-14.0, Tween80 9.0-10.0, BHT 0.3, sodium pyrosulfite 0.3, purified water 62-65 part.Select the father and mother in 20-22 age in week for kind of a fowl, the purifying daily ration of A of being deficient in vitamin of feeding is measured Serum Vitamin A content at interval of 1 all wing venous blood collections.After kind of a fowl serum vitamin A level is reduced to 0.60-0.85 μ mol/L, adjusts its absorption vitamin a level by drinking-water and be stabilized in the 600IU/kg daily ration.In this scope, do not affect its laying rate and incubation rate, plant the critical deficiency state that young fowl that egg hatches namely reaches vitamin A, and don't as for due to the too weak Deaths that occurs of physique.Go out the young fowl of the shell purifying age in days of A of being deficient in vitamin of feeding, coordinate the vitamin A micro emulsion drinking water, adjusting it, to take in vitamin A daily ration level be 120IU/kg, can keep the critical deficiency state of vitamin A of young fowl.
Described poultry is laying hen, broiler chicken, meat duck, goose and quail etc.
Compared with prior art, the construction method of the critical shortage animal model of poultry vitamin A disclosed by the invention has following advantage and marked improvement: 1. can obtain the critical shortage model of the young fowl vitamin A of 1 age in days, be convenient to carry out early stage experimental study.2. the animal model that builds by this method, in colony, regularity is high.3. the animal model that builds by this method had both had the characteristics of the critical shortage of vitamin A, had again enough anti-adversity abilities.4. this method is prepared the purifying daily ration of the A that is deficient in vitamin, adjust the absorption level of vitamin A by drinking-water, compare with the purifying daily ration of direct preparation low content vitamin A, convenient flexible, and the modeling that causes due to the vitamin A degraded after can avoiding daily ration to prepare is uncertain.
Embodiment
Be below specific embodiments of the invention, technical scheme of the present invention is done further the description, but protection scope of the present invention is not limited to these embodiment.Every do not deviate from the change of the present invention design or be equal to substitute include within protection scope of the present invention.
Embodiment one: the structure of the critical shortage animal model of meat chick vitamin A
Prepare the purifying daily ration of the A that is deficient in vitamin, comprise the raw material of following ratio of weight and number: casein 20.0, DL-METHIONINE 3.0, corn starch 15.0, sucrose 50.0, cellulose 5.0, corn oil 5.0, microelement pre-mix material 3.5, vitamin premix 1.0.Wherein, microelement pre-mix material is pressed the poultry nutrition standard preparation that U.S. NRC recommends announcement, and vitamin premix is not except containing vitamin A, and all the other compositions are all prepared by the poultry nutrition standard that U.S. NRC recommends.Prepare in addition the vitamin A microemulsion solution, comprise the raw material of following ratio of weight and number: oleovitamin A (content 1000000IU/g) 0.1, propane diols 10.0, Span60 12.0, and Tween80 10.0, and BHT 0.3, sodium pyrosulfite 0.3,62 parts of purified water.Select Luo Si 308 Parent stocks in 20 ages in week, the purifying daily ration of A of being deficient in vitamin of feeding is measured Serum Vitamin A content at interval of 1 all wing venous blood collections.Through the feeding experiment in 12 weeks, after kind chicken serum vitamin a level is reduced to the scope of 0.60-0.85 μ mol/L, adjusts its absorption vitamin a level by vitamin A microemulsion formulation drinking-water and be stabilized in the 600IU/kg daily ration.In this scope, do not affect its laying rate and incubation rate, plant the critical deficiency state that young fowl that egg hatches namely reaches vitamin A, and don't as for due to the too weak Deaths that occurs of physique.
By the fertilization kind egg that the kind chicken of above-mentioned experiment produces, the hatching of hatching program routinely, the serum VA of hatch chick meets the requirement of the critical shortage animal model of VA between 0.35-0.70 μ mol/L.
Embodiment two: checking and the application of the critical shortage animal model of meat chick vitamin A
Design following experiment by the constructed critical shortage model of meat chick vitamin A of the present invention in order further to verify.
1. materials and methods
1.1 experimental animal is chosen by resulting 1 age in days Luo Si 308 hatch chick 120 plumages of embodiment 1, be divided at random 3 groups, every group of 40 plumages: I group is critical shortage group, the purifying feed of feeding, and be 300IU/kg by vitamin A nanometer micro emulsion drinking water adjustment daily ration VA level; The II group is blank group, the purifying daily ration group of only feeding, not replenishing vitamins A; III organizes positive contrast, the purifying daily ration of feeding, and be 12000IU/kg by vitamin A nanometer micro emulsion drinking water adjustment daily ration VA level; Separately get chick 40 plumages of Luo Si 308 common species hatchings as negative control (IV group), the purifying daily ration of only feeding.
1.2 each treated animal list cage of test method is raised, free choice feeding is freely drunk water.By the experimental group of drinking-water replenishing vitamins, vitamin joins in appropriate drinking-water, drinks complete in 2-3 hour.In feeding process, each age in days Temperature and Humidity Control is pressed the program that Luo Si 308 livestock corporations are recommended.
1.3 sample collection and index determining are got at random 10 plumages broken ends respectively at every group of 1 age in days, 7 age in days and are got blood, get at random 10 wing venous blood collections respectively at every group of 14 age in days, 21 ages in days, 28 ages in days, 35 ages in days, 42 age in days.The blood sample separation of serum that gathers is by the high effective liquid chromatography for measuring vitamin A concentration.
2. result of the test
2.1 general health situation
The animal of test I group has 2 death during to off-test, and cuing open inspection, to show kidney pale, the typical critical shortage symptom of VA such as pawl, beak tarnish.The chicken of survival also shows the unrest of feather pine, growth retardation etc., meets the Symptoms of the critical shortage of poultry VA.
The experimental animal of test II group begins to occur more death owing to lacking VA in feed in the 3rd week, and the 4th week is most of dead, shows typical VA and lacks symptom.
Test III group is owing to having replenished high-caliber VA by drinking-water, health status and well-grown 1 example only occur dead in experimental period.
Test IV is organized in the 2nd week and begins to occur fragmentary death, and the 5th week is most of dead.Dead animal is cutd open inspection and shows typical VA shortage symptom.
2.2 serum VA content
In experimental period, the variation of each experimental group animal blood serum VA content as shown in Figure 1.
Description of drawings: Fig. 1 is each experimental group animal blood serum VA content tendency chart in experimental period.
As seen from Figure 1, I treated animal serum VA content remains on metastable level always between 0.50 μ mol/L-0.68 μ mol/L, and in colony, the variation of VA contents level is very little, meets the relevant requirements of the critical shortage animal model of poultry VA.
It is very fast that II treated animal serum VA content descends, namely be down in the 2nd week under critical shortage level (0.35 μ mol/L), colony between the different animals individuality serum VA level make a variation less.Illustrate that the animal model of this method foundation can be used for the correlative study of VA deficiency disease.
III treated animal serum VA level raises gradually, in colony between the different animals individuality serum VA level variation less, illustrate that the animal model that this method is set up can make good response to the additional VA of diet.
Be in higher level during IV treated animal serum VA content 1 age in days, reduce rapidly afterwards, colony in, Different Individual serum VA level makes a variation greatlyr, sets up in the model process and follows mortality.
3. experiment conclusion
By the critical shortage animal model of poultry VA that this method is set up, serum VA horizontal stable, content range meet the related index requirement of the critical shortage of VA.Animal pattern health status can satisfy the requirement of ordinary test, and the animal model of foundation has good response to the VA that replenishes, existing problem during the present animal model that effectively solves builds.
Claims (5)
1. the construction method of the critical shortage poultry of a vitamin A animal model, it is characterized in that: the kind fowl with the purifying Diet laying period of the A that is deficient in vitamin reduces its serum VA level to 0.60-0.85 μ mol/L, and making it remain on this level by vitamin A solution drinking-water, the kind egg of producing obtains the critical shortage poultry of the VA animal model of 1 age in days through hatching.
2. the method for claim 1, it is characterized in that, the purifying daily ration of the described A of being deficient in vitamin comprises the raw material of following ratio of weight and number: casein 15.0-25.0, DL-METHIONINE 1.5-4.5, corn starch 10.0-18.0, sucrose 40.0-60.0, cellulose 4.0-6.0, corn oil 4.0-6.0, microelement pre-mix material 3.5, vitamin premix 1.0; Described vitamin A solution comprises the raw material of following ratio of weight and number: oleovitamin A (content 1000000IU/g) 0.1, propane diols 10.0-15.0, Span60 10.0-15.0, Tween80 8.0-12.0, BHT 0.3, sodium pyrosulfite 0.3, purified water 60-70 part.
3. the method for claim 1, it is characterized in that, prepare the poultry nutrition standard preparation that purifying daily ration microelement pre-mix material used is recommended by U.S. NRC, vitamin premix is not except containing vitamin A, and all the other compositions are all prepared by the poultry nutrition standard that U.S. NRC recommends.
4. the method for claim 1, it is characterized in that, be the physique that keeps kind of the horizontal stable of fowl Serum Vitamin A and animal model to have enough health, dosage by drinking-water replenishing vitamins A solution calculates with daily ration concentration part by weight and is respectively 600IU/kg(kind fowl) and the 300IU/kg(chick).
5. poultry animal model as claimed in claim 1, is characterized in that, described poultry is laying hen, broiler chicken, meat duck, goose and quail etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100731958A CN103141433A (en) | 2013-03-07 | 2013-03-07 | Construction method for livestock animal model with critical lack of Vitamin A |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100731958A CN103141433A (en) | 2013-03-07 | 2013-03-07 | Construction method for livestock animal model with critical lack of Vitamin A |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103141433A true CN103141433A (en) | 2013-06-12 |
Family
ID=48539995
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013100731958A Pending CN103141433A (en) | 2013-03-07 | 2013-03-07 | Construction method for livestock animal model with critical lack of Vitamin A |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103141433A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2250099C2 (en) * | 2002-12-06 | 2005-04-20 | ООО "БиоТЭП" | Semakur-a complex preparation |
| CN101632679A (en) * | 2009-04-20 | 2010-01-27 | 邓盛齐 | Composite vitamin for injection and preparation method thereof |
| CN101668532A (en) * | 2007-04-25 | 2010-03-10 | 赛特克罗公司 | Method of treating vitamin d insufficiency and deficiency |
| CN101911923A (en) * | 2010-07-22 | 2010-12-15 | 重庆医科大学附属儿童医院 | Method for constructing preconception-starting vitamin A-deficiency female mouse and newborn mouse animal models |
| CN102038111A (en) * | 2010-12-27 | 2011-05-04 | 中牧实业股份有限公司 | Compound vitamin for egg-laying chicken |
| CN102048031A (en) * | 2010-12-27 | 2011-05-11 | 中牧实业股份有限公司 | Compound nutrient for poultry drinking water |
| JP4768870B1 (en) * | 2010-03-26 | 2011-09-07 | 昭治 藤江 | Livestock feed |
-
2013
- 2013-03-07 CN CN2013100731958A patent/CN103141433A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2250099C2 (en) * | 2002-12-06 | 2005-04-20 | ООО "БиоТЭП" | Semakur-a complex preparation |
| CN101668532A (en) * | 2007-04-25 | 2010-03-10 | 赛特克罗公司 | Method of treating vitamin d insufficiency and deficiency |
| CN101632679A (en) * | 2009-04-20 | 2010-01-27 | 邓盛齐 | Composite vitamin for injection and preparation method thereof |
| JP4768870B1 (en) * | 2010-03-26 | 2011-09-07 | 昭治 藤江 | Livestock feed |
| CN101911923A (en) * | 2010-07-22 | 2010-12-15 | 重庆医科大学附属儿童医院 | Method for constructing preconception-starting vitamin A-deficiency female mouse and newborn mouse animal models |
| CN102038111A (en) * | 2010-12-27 | 2011-05-04 | 中牧实业股份有限公司 | Compound vitamin for egg-laying chicken |
| CN102048031A (en) * | 2010-12-27 | 2011-05-11 | 中牧实业股份有限公司 | Compound nutrient for poultry drinking water |
Non-Patent Citations (1)
| Title |
|---|
| CLIVE E.WEST等: "Production of chickens with marginal vitamin A deficiency", 《BRITISH JOURNAL OF NUTRITION》, vol. 68, 31 December 1992 (1992-12-31) * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Bergoug et al. | Effect of pre-incubation and incubation conditions on hatchability, hatch time and hatch window, and effect of post-hatch handling on chick quality at placement | |
| Daryin et al. | Efficiency of using Echinacea purpurea in feeding laying hens of a parent flock. | |
| Lieboldt | PAPER I | |
| CN107711715A (en) | A kind of method for breeding of laying hen | |
| CN103461267A (en) | Method for farming chickens | |
| CN110192540A (en) | A kind of test method that the dry worm of black soldier flies influences performance in layers and Egg Quality | |
| CN107259176A (en) | Fowl pecks brick | |
| Okur et al. | Impacts of breeder age, storage time and setter ventilation program on incubation and post-hatch performance of broilers | |
| CN110100974A (en) | A kind of feed addictive and its application improving Layer Production Performance and Egg Quality | |
| RU2383208C2 (en) | Feed for young chicks | |
| Viola et al. | Influence of water restriction on the performance and organ development of young broilers | |
| CN104186406A (en) | Non-fasting forced molting method for laying hens | |
| CN103141433A (en) | Construction method for livestock animal model with critical lack of Vitamin A | |
| Hutt et al. | XI.—Studies in Embryonic Mortality in the Fowl. II. Chondrodystrophy in the Chick | |
| CN108850734A (en) | It is a kind of improve Nibea coibor air bladder quality artifical compound feed and its preparation | |
| CN102972344A (en) | Game cock hatching and breeding method | |
| CN105360067A (en) | Method for breeding of fat liver gooses | |
| Wang | The effects of different feeding program and inclusion of glycerol, glucose or sucrose in broiler starter diets on growth performance and intestinal development | |
| CN100569067C (en) | The young monkey of a kind of no-special pathogen experiment machin is from newborn method | |
| Setiyaningsih et al. | Effects of a Vitamins D and C Supplement on Performance, Hatchability, and Blood Profiles of Broiler Breeders | |
| RU2622921C2 (en) | Method for keeping laying hens of parent flock | |
| Parnian et al. | Effect of in ovo injection of nicotonic acid, pantothenic acid or folic acid on immune system and growth of broiler chickens. | |
| CN109221778A (en) | A kind of egg feedstuff and preparation method thereof improving immunity of layer chicken | |
| ÖZENTÜRK et al. | Comparison of performance parameters, stress, and immunity levels of native andcommercial layers reared in different cage densities in Turkey | |
| RU2771642C1 (en) | Method for growing chicken broilers with phytobiotics |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130612 |