CN103125480A - Loach sperm artificial storage method - Google Patents
Loach sperm artificial storage method Download PDFInfo
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- CN103125480A CN103125480A CN2011104016626A CN201110401662A CN103125480A CN 103125480 A CN103125480 A CN 103125480A CN 2011104016626 A CN2011104016626 A CN 2011104016626A CN 201110401662 A CN201110401662 A CN 201110401662A CN 103125480 A CN103125480 A CN 103125480A
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- loach
- sperm
- sperms
- sodium bicarbonate
- storage method
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Abstract
The invention relates to an aquaculture loach sperm artificial storage method. A robust male loach is selected; two testes are taken out, washed with normal saline and ground; small mortars are two in number and are respectively used for grinding of a pituitary gland and the testes; a sodium bicarbonate piece and a compound sulfamethoxazole tablet are prepared, ground, and fully dissolved with 250ml of the normal saline; sperms of the loach are placed into the dissolved sodium bicarbonate piece and the compound sulfamethoxazole tablet, and then held in a brown small glass bottle; and the small glass bottle is sealed and stored under a normal temperature. Under the normal temperature, in twelve months, the survival rate of the sperms is 90%, and in twenty-four months, the survival rate of the sperms is 80%.
Description
The present invention relates to the artificial store method of a kind of aquiculture animal loach sperm
Loach has the characteristics such as distribution is wide, strong stress resistance, easy cultivation in China, and has higher economic worth and medical value, have the title of " ginseng in water ".Though the propagation method of loach is many, adopt the natural propagation method easy, because being subject to the impact of many environmental factors, its fertilization rate is low.The loach natural spawning phase is longer, and benefit is not high.Also have some fertilized eggs not to be attached on the fish nest and suffer a loss, having influence on the seed pick-up rate.In the time of can not satisfying the breeding scale loach to the demand of seed.In the last few years, people often adopted artificial fecundation method to make up the deficiency of natural production.At present, for a large amount of Fertilized Eggs of Loachs that obtain, generally choose 2-3 age, healthy one-tenth loach without wound as kind of a loach.Female loach requires long 14-16 centimetre of body, more than body weight 18 grams, the large softness of abdomen, high resilience.Male loach requires long 10-12 centimetre of body, more than body weight 12 grams, can not squeeze seminal fluid, on pectoral fin, star-pursuing will be arranged.When taking artificial insemination, the male and female loach of injecting ocyodinic is supported respectively temporarily in Xiao Chi or net cage, in the time of water temperature 20-25 ℃, through 10-15 hour, gently pressed female loach belly to have the ovum grain to flow out, can carry out artificial insemination.Two kinds of methods are adopted in semen collection: a kind of is that the male loach belly of light pressure has seminal fluid to flow out; Can not squeeze seminal fluid or seminal fluid when not enough if another kind method is male loach, can take out spermary and fully shred, then wash out seminal fluid with physiological saline, be used for fertilization.Loach breeding adopts dry method to carry out artificial insemination usually, is about to " doing " seminal fluid and ovum and is mixed in a container, evenly stirs with feather or hand, stirs gently 1-2 minute after adding water again, completes artificial insemination and carries out upper nest.In addition, through the loach parent population of artificial induced spawning, also available test-tube method obtains fertilized egg.When the male and female parent population begins to oestrus, it has been caught, under the condition without direct sunlight, ovum and sperm are clamp-oned in preprepared enamel bowl or basin, stir with feather immediately, then add a small amount of clear water, make smart ovum fully contact, be fertilized.The fertilized egg that obtains is sprinkling upon on the fish nest, or takes off and put into incubator after glutinous and hatch.But, because the loach sperm is difficult for preserving, easily inactivation, therefore, carry out artificial loach breeding in a large number, at first a large amount of Fertilized Eggs of Loachs to be arranged, want a large amount of Fertilized Eggs of Loachs, must carry out artificial insemination, and carry out artificial insemination, a large amount of loach sperm stocks must be arranged, and is a problem of needing solution badly and make every effort to sperm storage.
Propagate the not enough problem with being difficult for preserving of middle loach sperm storage artificially in order to overcome existing loach, the invention provides the artificial store method of a kind of loach sperm.
The technical solution adopted for the present invention to solve the technical problems is: choose 1 of healthy and strong male kind of loach, take out 2 spermaries, clean with physiological saline, little mortar grinds spermary; Get 1 of Sodium Bicarbonate Tablets, 1 of compound sulfamethoxazole and trimethoprim tablets, two kinds of tablets are ground, put into physiological saline 250ml and fully dissolve, then the loach sperm is put into solution fully mix, the brown vial of packing into, sealing is preserved at-5 ℃ of-12 ℃ of temperature.
The invention has the beneficial effects as follows, when a large amount of loach sperm can be provided under extensive raising condition, facilitate the preservation of loach sperm, and improve the survival rate of sperm, method is simple.
Description of drawings
The present invention is further described below in conjunction with drawings and Examples.
Fig. 1 is implementing procedure figure of the present invention.
Embodiment: choose 1 of healthy and strong male kind of loach, take out 2 spermaries, clean with physiological saline, little mortar grinds spermary; Get Sodium Bicarbonate Tablets 0.5g (tablet, Tianjin Lisheng Pharmaceutical Co., Ltd. produces, the accurate word H12020220 of traditional Chinese medicines); Compound sulfamethoxazole and trimethoprim tablets 0.5g (tablet, Huazhong Pharmaceutical Co., Ltd. produces, the accurate word H42022487 of traditional Chinese medicines); Two kinds of tablets are ground, put into sodium chloride (physiological saline) 250ml and fully dissolve, the loach sperm is put into solution, the brown vial of packing into, sealing is preserved, and under-5 ℃ of-12 ℃ of temperature regimes, preserves in 12 months sperm survival rate 90%; In 24 months, sperm survival rate 80%.
The below is that 2009--2011 is with the experimental data of a collection of loach sperm storage.
Claims (2)
1. artificial store method of loach sperm.It is characterized in that: get male kind of loach spermary of 1 stalwartness, clean with physiological saline, mortar grinds.
2. the artificial store method of a kind of loach sperm according to claim 1, it is characterized in that: get 1, sodium bicarbonate tablet, 1 of compound sulfamethoxazole and trimethoprim tablets agent, two kinds of tablets are pulverized through mortar, putting into physiological saline 250ml fully dissolves, the loach sperm is put into solution, fully mix the brown vial sealing of packing into.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104016626A CN103125480A (en) | 2011-11-22 | 2011-11-22 | Loach sperm artificial storage method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104016626A CN103125480A (en) | 2011-11-22 | 2011-11-22 | Loach sperm artificial storage method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103125480A true CN103125480A (en) | 2013-06-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2011104016626A Pending CN103125480A (en) | 2011-11-22 | 2011-11-22 | Loach sperm artificial storage method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105393970A (en) * | 2015-12-12 | 2016-03-16 | 重庆华楷水产养殖有限公司 | Loach breeding method |
| CN105557683A (en) * | 2016-01-15 | 2016-05-11 | 清远职业技术学院 | Loach sperm preservation method and corresponding loach sperm preservation solution |
| CN113273567A (en) * | 2021-06-04 | 2021-08-20 | 大连海洋大学 | Low-temperature preservation liquid for patinopecten yessoensis sperms and preservation and use method |
-
2011
- 2011-11-22 CN CN2011104016626A patent/CN103125480A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 万全等: "5种配方和5种糖类对大鳞副泥鳅精子保存效果的研究", 《水生态学杂志》 * |
| 万全等: "5种配方和5种糖类对大鳞副泥鳅精子保存效果的研究", 《水生态学杂志》, vol. 2, no. 5, 15 September 2009 (2009-09-15), pages 85 - 89 * |
| 张金龙等: "抗生素对猪精液常温保存效果的影响", 《黑龙江动物繁殖》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105393970A (en) * | 2015-12-12 | 2016-03-16 | 重庆华楷水产养殖有限公司 | Loach breeding method |
| CN105557683A (en) * | 2016-01-15 | 2016-05-11 | 清远职业技术学院 | Loach sperm preservation method and corresponding loach sperm preservation solution |
| CN113273567A (en) * | 2021-06-04 | 2021-08-20 | 大连海洋大学 | Low-temperature preservation liquid for patinopecten yessoensis sperms and preservation and use method |
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Application publication date: 20130605 |