CN105393970A - Loach breeding method - Google Patents
Loach breeding method Download PDFInfo
- Publication number
- CN105393970A CN105393970A CN201510935076.8A CN201510935076A CN105393970A CN 105393970 A CN105393970 A CN 105393970A CN 201510935076 A CN201510935076 A CN 201510935076A CN 105393970 A CN105393970 A CN 105393970A
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- China
- Prior art keywords
- loach
- female
- injection
- male
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a loach breeding method. The method comprises the following steps of (1) selecting seeds, (2) inducing spawning: injecting spawning induction liquid after male and female loaches are placed into a hatching barrel for 24 hours, (3) inseminating: squeezing eggs of the female loach and taking out a testis of the male loach after the spawning induction liquid is injected for 13 to 15 hours, adding gluecose solution to a testis mucous membrane and grinding for one minute, removing residual seminal vesicle, absorbing the seminal fluid into an injection tube, placing the female loach eggs to an egg container and injecting the seminal fluid, and pouring the germ cells to aerated mineral water and continuously stirring for two minutes, (4) hatching, and (5) spreading seedlings: 3 to 5 days later and during the temperature transition time of the morning and the night, converting the loach seedlings in a hatching pool to a seedling-spreading pool until the loaches seedlings grow 3 to 5cm. High loach fertility rate and high survival rate can be achieved by the use of the method.
Description
Technical field
The present invention relates to the breeding method of a kind of loach.
Background technology
Loach (Misgurnusanguillicaudatus) is the common Small Freshwater Fishes of China, its fine and tender taste, delicious, nutritious, and has higher medical value, is described as " in water ginseng ".In recent years, the market demand of loach increases day by day, but due to overfishing for many years, add that natural water area ecotope goes from bad to worse, the stock number of the wild loach of China is faced with exhausted danger.From the nineties in last century, breeding of loach is progressively developed in China with aquaculture, has become the aquatic products economic fish that China is important at present.The small-sized economic fish of one that loach is newly risen as China, has vast potential for future development.Therefore, the genetic breeding research of loach has important economic implications.But in prior art, fertilization rate, the survival rate of loach breeding are but always lower, and this directly limit the scale development of loach breeding.
Therefore those skilled in the art are devoted to develop a kind of fertilization rate, loach breeding method that survival rate is high.
Summary of the invention
Because the above-mentioned defect of prior art, technical problem to be solved by this invention is to provide a kind of fertilization rate, loach breeding method that survival rate is high.
For achieving the above object, the invention provides a kind of loach breeding method, comprise the following steps:
1) choose seeds: in the annual 3-10 month, select the loach of 250-350g as kind of a loach, wherein the proportioning of male loach and female loach is 1:6; Male loach and female loach are inserted hatching pail respectively;
2) hasten parturition: male loach and female loach inserted hatching pail after 24 hours, inject liquid of hastening parturition, liquid of hastening parturition comprises 1 part of DOM injection, 1 part of injection Luteinizing hormone releasing-hormone A2,1 portion of chorionic gonadotrophin, 30 portions of glucose injections; Every loach injection 0.4ml; After injection, male loach and female loach divide being filled to station support bucket;
3) be fertilized: injection is squeezed ovum to female loach, taken out the spermary of public loach after hastening parturition liquid 13-15 hour; Spermary mucous membrane is added glucose solution and grinds 1 minute, remove remaining seminal vesicle; Seminal fluid is sucked in injection-tube; Female loach ovum is inserted and contains in egg apparatus, and inject seminal fluid, stir 1 minute with the feather of having sterilized simultaneously; Fertilized egg is poured in aeration mineral water, continue stirring 2 minutes;
4) hatch: the mineral water comprising fertilized egg is sent into hatchery; Water in hatchery is aeration underground water, and is heated to 23-28 DEG C; After 30-35 hour, loach breaks ovum, puts into opening material feeding, and opening material comprises lactic acid, yolk;
5) spread out after seedling: 3-5 days, in the morning or the temperature transit time in evening, the mud fish fry in hatchery is proceeded in seedling pond, stand and support to 3-5cm.
Preferably, spread out seedling pond in described step 3) 24 hours before carry out desinsection; Desinsection, after 24 hours, utilizes lime to detoxify; Removing toxic substances 24 hours after, utilize bacillus, lactic acid bacteria to stand seedling pond in water carry out rich water.
The invention has the beneficial effects as follows: the present invention makes the fertilization rate of loach high, and survival rate is high.
Embodiment
Below in conjunction with embodiment, the invention will be further described: a kind of loach breeding method, comprises the following steps:
1) choose seeds: in the annual 3-10 month, select the loach of 250-350g as kind of a loach, wherein the proportioning of male loach and female loach is 1:6; Male loach and female loach are inserted hatching pail respectively;
2) hasten parturition: male loach and female loach inserted hatching pail after 24 hours, inject liquid of hastening parturition, liquid of hastening parturition comprises 1 part of injection DOM (DOM), 1 part of injection Luteinizing hormone releasing-hormone A2 (LHRH-A2), 1 part of chorionic gonadotrophin (HCG), 30 portions of glucose injections; Every loach injection 0.4ml; After injection, male loach and female loach divide being filled to station support bucket;
3) be fertilized: injection is squeezed ovum to female loach, taken out the spermary of public loach after hastening parturition liquid 13-15 hour; Spermary mucous membrane is added glucose solution and grinds 1 minute, remove remaining seminal vesicle; Seminal fluid is sucked in injection-tube; Female loach ovum is inserted and contains in egg apparatus, and inject seminal fluid, stir 1 minute with the feather of having sterilized simultaneously; Fertilized egg is poured in aeration mineral water, continue stirring 2 minutes;
4) hatch: the mineral water comprising fertilized egg is sent into hatchery; Water in hatchery is aeration underground water, and is heated to 23-28 DEG C; After 30-35 hour, loach breaks ovum, puts into opening material feeding, and opening material comprises lactic acid, yolk;
5) spread out after seedling: 3-5 days, in the morning or the temperature transit time in evening, the mud fish fry in hatchery is proceeded in seedling pond, stand and support to 3-5cm.
Stand seedling pond in described step 3) 24 hours before carry out desinsection; Desinsection, after 24 hours, utilizes lime to detoxify; Removing toxic substances 24 hours after, utilize bacillus, lactic acid bacteria to stand seedling pond in water carry out rich water.
Empirical tests, adopts above-mentioned breeding method, and the fertilized egg of loach can be made to improve 30%, and survival rate improves 20%.
More than describe preferred embodiment of the present invention in detail.Should be appreciated that those of ordinary skill in the art just design according to the present invention can make many modifications and variations without the need to creative work.Therefore, all technical staff in the art, all should by the determined protection domain of claims under this invention's idea on the basis of existing technology by the available technical scheme of logical analysis, reasoning, or a limited experiment.
Claims (2)
1. a loach breeding method, is characterized in that comprising the following steps:
1) choose seeds: in the annual 3-10 month, select the loach of 250-350g as kind of a loach, wherein the proportioning of male loach and female loach is 1:6; Male loach and female loach are inserted hatching pail respectively;
2) hasten parturition: male loach and female loach inserted hatching pail after 24 hours, inject liquid of hastening parturition, liquid of hastening parturition comprises 1 part of DOM injection, 1 part of injection Luteinizing hormone releasing-hormone A2,1 portion of chorionic gonadotrophin, 30 portions of glucose injections; Every loach injection 0.4ml; After injection, male loach and female loach divide being filled to station support bucket;
3) be fertilized: injection is squeezed ovum to female loach, taken out the spermary of public loach after hastening parturition liquid 13-15 hour; Spermary mucous membrane is added glucose solution and grinds 1 minute, remove remaining seminal vesicle; Seminal fluid is sucked in injection-tube; Female loach ovum is inserted and contains in egg apparatus, and inject seminal fluid, stir 1 minute with the feather of having sterilized simultaneously; Fertilized egg is poured in aeration mineral water, continue stirring 2 minutes;
4) hatch: the mineral water comprising fertilized egg is sent into hatchery; Water in hatchery is aeration underground water, and is heated to 23-28 DEG C; After 30-35 hour, loach breaks ovum, puts into opening material feeding, and opening material comprises lactic acid, yolk;
5) spread out after seedling: 3-5 days, in the morning or the temperature transit time in evening, the mud fish fry in hatchery is proceeded in seedling pond, stand and support to 3-5cm.
2. loach breeding method as claimed in claim 1, is characterized in that: seedling pond, stand is in described step 3) 24 hours before carry out desinsection; Desinsection, after 24 hours, utilizes lime to detoxify; Removing toxic substances 24 hours after, utilize bacillus, lactic acid bacteria to stand seedling pond in water carry out rich water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510935076.8A CN105393970A (en) | 2015-12-12 | 2015-12-12 | Loach breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510935076.8A CN105393970A (en) | 2015-12-12 | 2015-12-12 | Loach breeding method |
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| Publication Number | Publication Date |
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| CN105393970A true CN105393970A (en) | 2016-03-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510935076.8A Pending CN105393970A (en) | 2015-12-12 | 2015-12-12 | Loach breeding method |
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| CN (1) | CN105393970A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172146A (en) * | 2016-09-21 | 2016-12-07 | 南宁学院 | A kind of artificial insemination method of rainbow trout |
| CN107517893A (en) * | 2017-09-29 | 2017-12-29 | 清远职业技术学院 | A kind of method bred using warm mineral water development loach fish fry artificial with cultivation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102599091A (en) * | 2012-03-26 | 2012-07-25 | 浙江海洋学院 | Hybrid propagation method and fertilized ovum hatching device for loach |
| CN103109767A (en) * | 2013-03-08 | 2013-05-22 | 江苏省农业科学院宿迁农科所 | Loach fish fry artificial cultivation method |
| CN103125480A (en) * | 2011-11-22 | 2013-06-05 | 湖北天门四海生态科技有限公司 | Loach sperm artificial storage method |
-
2015
- 2015-12-12 CN CN201510935076.8A patent/CN105393970A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125480A (en) * | 2011-11-22 | 2013-06-05 | 湖北天门四海生态科技有限公司 | Loach sperm artificial storage method |
| CN102599091A (en) * | 2012-03-26 | 2012-07-25 | 浙江海洋学院 | Hybrid propagation method and fertilized ovum hatching device for loach |
| CN103109767A (en) * | 2013-03-08 | 2013-05-22 | 江苏省农业科学院宿迁农科所 | Loach fish fry artificial cultivation method |
Non-Patent Citations (1)
| Title |
|---|
| 高峰等: "《泥鳅生态养殖》", 31 October 2013, 湖南科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172146A (en) * | 2016-09-21 | 2016-12-07 | 南宁学院 | A kind of artificial insemination method of rainbow trout |
| CN107517893A (en) * | 2017-09-29 | 2017-12-29 | 清远职业技术学院 | A kind of method bred using warm mineral water development loach fish fry artificial with cultivation |
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Application publication date: 20160316 |