CN103113477A - Method for preparing starch glue powder from medium-temperature amylase - Google Patents
Method for preparing starch glue powder from medium-temperature amylase Download PDFInfo
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- CN103113477A CN103113477A CN2013100654025A CN201310065402A CN103113477A CN 103113477 A CN103113477 A CN 103113477A CN 2013100654025 A CN2013100654025 A CN 2013100654025A CN 201310065402 A CN201310065402 A CN 201310065402A CN 103113477 A CN103113477 A CN 103113477A
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Abstract
The invention relates to a method for preparing starch glue powder from medium-temperature amylase. The method comprises the following steps of: (1) carrying out starch size mixing: mixing starch serving as a raw material with water to be uniform according to a mass-volume ratio of 1: 1 so as to prepare starch suspension; (2) carrying out enzymolysis reaction: adding alpha-amylase which is 0.1-0.15% of the weight of the starch into the starch suspension prepared in the step (1), continuously stirring, carrying out enzymolysis for 10 minutes at the temperature of 50 DEG C, immediately adding 10M/L NaOH which is 0.05-0.1% of the total volume, and adding anhydrous ethanol which is 40-60% of the total volume, mixing uniformly and then terminating reaction; and (3) after suction filtration, drying, regrinding, and drying at the temperature of 120 DEG C until the alpha-amylase is inactivated, thereby obtaining finished starch glue powder. The starch glue powder is good in fluidity, non-toxic, odorless, moderate in viscosity, good in initial adhesion and self-adjustable in viscosity range and can be widely applied to the carton industry.
Description
Technical field
The present invention relates to a kind of method that middle temperature amylase prepares the starch rubber powder.
Background technology
Starch Adhesive is one of tackiness agent of the present carton industry large usage quantity that is used for place of water glass.At present domestic most amylan tackiness agent is that starch is directly made glue, not only transportation inconvenience, and gelatin viscosity stability in time prolongation and variation is difficult for stored for a long timely, be only suitable in self-produced personal.In addition, the solids content of Starch Adhesive is lower, cardboard is extended time of drying, thereby cause production efficiency to reduce.If it is bad that working condition is controlled, can cause that also Starch Adhesive lost efficacy in storage period.Address these problems, need to make the starch rubber powder, utilize α-amylase that the starch long chain molecule is hydrolyzed to short chain molecule, make the viscosity controller of starch make instant dissolving glue powder in certain scope, the user can allocate voluntarily as required glue and be applied to the carton industry.
α-amylase is a kind of important hydrolyzed starch class zymin, and output is larger in China, when it acts on starch, cuts the α of starch molecule inside-Isosorbide-5-Nitrae glycosidic link and makes starch generate dextrin, oligose etc. in random mode.Existing enzyme transforming process is produced amylan, and temperature of reaction requires more than 60 ℃ more, consume energy higher, and the easy gelatinization of starch with this understanding, can't make the starch rubber powder by suction filtration.
Summary of the invention
The object of the present invention is to provide a kind of middle temperature amylase to prepare the method for starch rubber powder, be convenient to transportation and preserve with the amylan rubber powder that the method is prepared into, can replace general liquid starchy tackiness agent, and compare with like product, have the characteristics such as cost is low, application is convenient and swift, environmental protection.
The present invention is achieved through the following technical solutions above-mentioned purpose: a kind of middle temperature amylase prepares the method for starch rubber powder, comprises the steps:
(1) starch is sized mixing: be the ratio of 1:1 mixes starch material and water and makes starch suspension in mass volume ratio,
(2) enzyme digestion reaction: the α-amylase that adds the 0.1-0.15% of starch weight in the starch suspension that mixes up to step 1), and continual stirring, enzymolysis is 10 minutes at the temperature of 50 ℃, add immediately the NaOH of 10M/L by the 0.05-0.1% of cumulative volume, the dehydrated alcohol mixing that adds again cumulative volume 40-60%, termination reaction.
(3) suction filtration post-drying is pulverized again again in 120 ℃ of oven dry, until the α-amylase deactivation namely obtains starch rubber powder finished product.
Described amylase is can be at the mesophilicα-diastase of the living tapioca (flour) of enzymolysis below 60 ℃.
Described starch is tapioca (flour).
The application that described middle temperature amylase prepares the starch rubber powder is after described starch rubber powder is made into the suspension of 10-20% by required viscosity, in 95 ℃ of high temperature gelatinizations, during continual stirring 10 minutes, until gelatinization fully can be used in direct gluing.
The invention has the advantages that:
1, be the starch rubber powder due to what prepare, steady quality is easy to transportation and stores, and is easy to use.
2, this finished product composition is simple, nontoxic, but the range of viscosities Self-adjustment can be widely used in the carton industry.
3, less investment, technique is simple, and cost is low.
Embodiment
By the following examples technical scheme of the present invention is described further.
Embodiment 1
Middle temperature amylase of the present invention prepares the method for starch rubber powder, comprises the steps:
1, starch is sized mixing: be that the ratio of 1:1 mixes starch material and water and makes starch suspension in mass volume ratio;
2, enzyme digestion reaction: the 0.1-0.15% by starch weight in the starch suspension that mixes up to step 1) adds α-amylase, and continual stirring, enzymolysis is 10 minutes at 50 ℃ of temperature, add immediately the NaOH of 10M/L according to the 0.05-0.1% of cumulative volume, the dehydrated alcohol mixing 30 seconds that adds again cumulative volume 40-60%, termination reaction.
Illustrate: add the dehydrated alcohol purpose to be in this step: 1. dehydrated alcohol and NaOH acting in conjunction suppress enzyme digestion reaction; 2. can shorten 50 ℃ of drying times, guarantee the stability of starch rubber powder, the shortening time reaches more than one times, and the ethanolic soln after use can be recycled again.
3, suction filtration was dried 3-5 hour for 50 ℃, again pulverized again and dried 3-4 hour under 120 ℃, until the α-amylase deactivation namely obtains starch rubber powder finished product.
Embodiment 2
1, starch is sized mixing: be that the ratio of 1:1 mixes starch material and water and makes starch suspension in mass volume ratio;
2, enzyme digestion reaction: the 0.1-0.15% by starch weight in the starch suspension that mixes up to step 1) adds α-amylase, and continual stirring, hydrolysis temperature is dropped to 40 ℃ of enzymolysis 12 minutes, add NaOH and dehydrated alcohol termination reaction according to method in embodiment 1 immediately.
3, suction filtration was dried 3-5 hour for 50 ℃, again pulverized again and dried by the fire 3-4 hour under 120 ℃, until the α-amylase deactivation namely obtains starch rubber powder finished product.
Embodiment 3
1, starch is sized mixing: be that the ratio of 1:1.5 mixes starch material and water and makes starch suspension in mass volume ratio;
2, enzyme digestion reaction: add α-amylase by 0.1% of starch weight in the starch suspension that mixes up to step 1), and continual stirring, at 50 ℃ of temperature, enzymolysis time is adjusted to 8 minutes, adds NaOH and dehydrated alcohol termination reaction according to method in embodiment 1 immediately.
3, suction filtration was dried 3-5 hour for 50 ℃, again pulverized again and dried by the fire 3-4 hour under 120 ℃, until the α-amylase deactivation namely obtains starch rubber powder finished product.
The using method of starch rubber powder:
After embodiment 1,2,3 gained starch rubber powders being made into 10% suspension, in 95 ℃ of high temperature gelatinizations, during continual stirring 6-10 minute, until gelatinization fully can be used in direct gluing.
Described starch rubber powder finished product is white powder, be made into and record the pH value after suspension liquid and be 8.5-9.0, surveying the amylan range of viscosities after gelatinization under the condition of 50 ℃ is 0.027-0.084PaS, initial bonding strength detects and is 70-100%, this amylan good fluidity, nonpoisonous and tasteless, modest viscosity, initial bonding strength is good, meets the carton industry requirement.
Simultaneously will be under this starch rubber powder finished product normal temperature preserve two days, record after sample pH value value and gelatinization viscosity still within measurement range, illustrate that this sample stability is good, is easy to long-term preservation and transportation.
Claims (4)
1. warm amylase prepares the method for starch rubber powder in one kind, it is characterized in that, the method comprises the steps:
(1) starch is sized mixing: be the ratio of 1:1 mixes starch material and water and makes starch suspension in mass volume ratio,
(2) enzyme digestion reaction: the α-amylase that adds the 0.1-0.15% of starch weight in the starch suspension that mixes up to step 1), and continual stirring, enzymolysis is 10 minutes at the temperature of 50 ℃, add immediately the NaOH of 10M/L by the 0.05-0.1% of cumulative volume, the dehydrated alcohol mixing 30 seconds that adds again cumulative volume 40-60%, termination reaction
(3) 50 ℃ of oven dry 3-5 hour after suction filtration are pulverized again again in 120 ℃ of oven dry, until the α-amylase deactivation namely obtains starch rubber powder finished product.
2. middle temperature amylase according to claim 1 prepares starch rubber powder method, it is characterized in that: this amylase is can be at the mesophilicα-diastase of the living tapioca (flour) of enzymolysis below 60 ℃.
3. middle temperature amylase according to claim 1 prepares starch rubber powder method, and it is characterized in that: described starch is tapioca (flour).
4. the middle temperature amylase claimed in claim 1 application for preparing the starch rubber powder, it is characterized in that: after described starch rubber powder is made into the suspension of 10-20% by required viscosity, in 95 ℃ of high temperature gelatinizations, during continual stirring 10 minutes, until gelatinization fully can be used in direct gluing.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310065402.5A CN103113477B (en) | 2013-03-01 | 2013-03-01 | Method for preparing starch glue powder from medium-temperature amylase |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310065402.5A CN103113477B (en) | 2013-03-01 | 2013-03-01 | Method for preparing starch glue powder from medium-temperature amylase |
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| CN103113477A true CN103113477A (en) | 2013-05-22 |
| CN103113477B CN103113477B (en) | 2015-03-11 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6051700A (en) * | 1999-02-11 | 2000-04-18 | Grain Processing Corporation | Process and method for hydroxyalkylation of starch and hydroxyalkyl starch prepared accordingly |
| CN1911966A (en) * | 2006-08-23 | 2007-02-14 | 华南理工大学 | Pretreatment method of starch |
| CN101509211A (en) * | 2009-03-13 | 2009-08-19 | 东莞市汇美淀粉科技有限公司 | Method of preparing cation painting and coating starch |
| CN101611838A (en) * | 2008-06-23 | 2009-12-30 | 广东海洋大学 | The method of preparing maltodextrin by enzymolyzing cassava starch |
| CN101649582A (en) * | 2009-06-23 | 2010-02-17 | 东莞玖龙纸业有限公司 | Method for producing starch glue by low-temperature fast enzymatic conversion |
| CN101979763A (en) * | 2010-10-22 | 2011-02-23 | 东莞理文造纸厂有限公司 | Method for making papermaking starch glue |
-
2013
- 2013-03-01 CN CN201310065402.5A patent/CN103113477B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6051700A (en) * | 1999-02-11 | 2000-04-18 | Grain Processing Corporation | Process and method for hydroxyalkylation of starch and hydroxyalkyl starch prepared accordingly |
| CN1911966A (en) * | 2006-08-23 | 2007-02-14 | 华南理工大学 | Pretreatment method of starch |
| CN101611838A (en) * | 2008-06-23 | 2009-12-30 | 广东海洋大学 | The method of preparing maltodextrin by enzymolyzing cassava starch |
| CN101509211A (en) * | 2009-03-13 | 2009-08-19 | 东莞市汇美淀粉科技有限公司 | Method of preparing cation painting and coating starch |
| CN101649582A (en) * | 2009-06-23 | 2010-02-17 | 东莞玖龙纸业有限公司 | Method for producing starch glue by low-temperature fast enzymatic conversion |
| CN101979763A (en) * | 2010-10-22 | 2011-02-23 | 东莞理文造纸厂有限公司 | Method for making papermaking starch glue |
Non-Patent Citations (1)
| Title |
|---|
| 杨昌鹏主编: "《酶制剂生产与应用》", 31 August 2006, 中国环境科学出版社 * |
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