CN103103107B - Convenient body fluid cell precipitation instrument and using method thereof - Google Patents
Convenient body fluid cell precipitation instrument and using method thereof Download PDFInfo
- Publication number
- CN103103107B CN103103107B CN201310027919.5A CN201310027919A CN103103107B CN 103103107 B CN103103107 B CN 103103107B CN 201310027919 A CN201310027919 A CN 201310027919A CN 103103107 B CN103103107 B CN 103103107B
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- body fluid
- collection tube
- slide glass
- filter paper
- precipitation
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- 210000001124 body fluid Anatomy 0.000 title claims abstract description 49
- 239000010839 body fluid Substances 0.000 title claims abstract description 49
- 238000001556 precipitation Methods 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 12
- 239000011521 glass Substances 0.000 claims abstract description 41
- 238000004049 embossing Methods 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 238000004062 sedimentation Methods 0.000 claims description 5
- 238000004043 dyeing Methods 0.000 claims description 4
- 230000001413 cellular effect Effects 0.000 claims description 3
- 239000006059 cover glass Substances 0.000 claims description 3
- 230000002209 hydrophobic effect Effects 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- 238000010079 rubber tapping Methods 0.000 claims description 3
- 238000005204 segregation Methods 0.000 claims description 3
- 238000003780 insertion Methods 0.000 abstract 1
- 230000037431 insertion Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 30
- 238000013461 design Methods 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 206010003445 Ascites Diseases 0.000 description 2
- 206010048612 Hydrothorax Diseases 0.000 description 2
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 125000003003 spiro group Chemical group 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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Abstract
The invention provides a convenient body fluid cell precipitation instrument and a using method thereof, and relates to equipment for collecting body fluid cells and the using method thereof. The precipitation instrument with the structure comprises a precipitation seat, a collection pipe and a pressurization nut, wherein a rubber gasket is mounted in a circular groove at the upper part of the precipitation seat; when the precipitation instrument is used, a glass slide shielded by a plurality of layers of filter paper with open holes is fixed on the rubber gasket through an insertion opening; the collection pipe is positioned on the glass slide; and the pressurization nut is sleeved on the collection pipe and is connected with the precipitation seat through screws. The precipitation instrument provided by the invention has the characteristics of being novel in structure, simple and convenient to machine, small and exquisite in structure, simple to operate, low in cost, convenient to store and the like, thereby being a novel convenient body fluid cell precipitation instrument with the integrated performance of economy and practicability.
Description
Technical field
Convenient body fluid cell sedimentograph of the present invention and using method thereof, relate to a kind of equipment and using method thereof of collecting body fluid cell.
Background technology
At present, body fluid cell both domestic and external is collected and is mostly adopted test tube centrifugation method, complicated operation, instrument cost is higher, floor space is large, and the process of getting again sedimentation cell after centrifugal has also increased the chance of cytoclasis, and can not be widely used in small-size laboratory and basic hospital.After body fluid cell is in vitro, easily dissolves, destroy, therefore, after sample is in vitro, making immediately cell precipitation sheet is that further dyeing, Accurate Diagnosis institute are necessary.For existing problem in above-mentioned prior art, a kind of novel convenient body fluid cell sedimentograph of research and design, is very necessary thereby overcome existing problem in prior art.
Summary of the invention
In view of existing problem in above-mentioned prior art, the present invention has designed small and exquisite, convenient, practical, the inexpensive body fluid cell sedimentograph of a kind of volume, be conducive to the quick collection of body fluid (being mainly used in cerebrospinal fluid, hydrothorax, ascites, urine etc.) cell, directly body fluid cell is deposited on slide glass, do not destroy the form of cell, can be at situation of all-level hospitals, the widely used convenient body fluid cell sedimentograph in laboratory.Exist in prior art in order to solve: equipment volume is large, complicated operation, cost are high and separate after extract the problems such as cell of easily destroying.
Technical solution of the present invention is achieved in that
Convenient body fluid cell sedimentograph of the present invention; Its structure is that described convenient body fluid cell sedimentograph comprises precipitation seat, collection tube and pressurization nut; In the circular groove on precipitation seat top, rubber pad is housed; Collection tube is placed in groove, and pressurization spiro cap, in the outside of collection tube, is connected with precipitation seat by screw thread.
Precipitation seat of the present invention is right cylinder, is processed with circular groove on the top of precipitation seat, is processed with slide glass socket in the middle and upper part of precipitation seat, and the bottom of slide glass socket is higher than the inner bottom part of circular groove; Top at precipitation seat is processed with outside screw; The lower external face embossing processing of precipitation seat.
Collection tube of the present invention is the cylinder table of falling T-shape, and the external diameter of its underpart round platform is slightly less than the internal diameter of circular groove; The top of top cylinder is processed with outside screw; The middle part of collection tube is processed with through hole.
The inner side of pressurization nut of the present invention is processed with and the internal thread that precipitates a upper external thread and be meshed, and is screwed tight and collection tube is fixed in circular groove with precipitation seat by the screw thread nut that makes to pressurize; Top at pressurization nut is processed with collection tube through hole, and the internal diameter of collection tube through hole is slightly larger than the external diameter of collection tube top cylinder; The outside surface of pressurization nut does embossing processing.
The thickness of rubber pad of the present invention is greater than circular groove bottom to the height between slide glass socket.
The using method of convenient body fluid cell sedimentograph of the present invention:
A, rubber pad is put in the circular groove of precipitation seat;
B, by folded filter paper multilayer, and perforate in the middle, the diameter in hole is identical with the diameter of the through hole of collection tube; The size of folded filter paper is identical with the size of slide glass; Filter paper is placed on slide glass and is together inserted in slide glass socket, make the perforate of filter paper be centered close to the center of circular groove;
C, collection tube is put in circular groove, and through hole is alignd with the perforate on filter paper; To pressurize spiro cap on collection tube, and be threaded and screw with precipitation seat, slide glass and filter paper will be fixed on convenient body fluid cell sedimentograph;
D, the 1 milliliter of body fluid mixing is poured in the through hole of collection tube, i.e. the tapping of the filter paper on slide glass, then a cover glass is covered on the through hole of collection tube, prevent that body fluid is contaminated; Moisture in body fluid is absorbed by filter paper, and the cell natural sedimentation in body fluid is on slide glass;
E, after body fluid and cellular segregation, open pressurization nut, take out collection tube, extract slide glass and filter paper out, separately filter paper and slide glass, draw circle at filter paper perforate position, slide glass center with hydrophobic pen, select form and then the classification that after suitable dye liquor dyeing, can examine under a microscope body fluid cell according to coloured differently object.
Advantage of the present invention is apparent, is mainly manifested in:
1, design of the present invention, is conducive to the quick collection of body fluid (being mainly used in cerebrospinal fluid, hydrothorax, ascites, urine etc.) cell, directly body fluid cell is deposited on slide glass, does not destroy the form of cell, keeps the integrity of cell;
2, design of the present invention is smaller and more exquisite, convenient, practical and cheap, and applicable situation of all-level hospitals, laboratory are widely used;
3, simple in structure, the small volume of the present invention, routine maintenance easy to clean, dry after collection keeping;
4, design of the present invention is used, and has saved the maintaining cost of equipment, has saved expenses, has reduced use cost.
5, the present invention utilizes Adsorption of Filter Paper moisture cell natural sedimentation method, without using electric power, has reduced the wasting of resources;
6, simple in structure, the metal material of the present invention, long service life, material can be recycled, environmental protection more.
The present invention has novel structure, it is easy to process, volume is small and exquisite, simple to operate, cheap, be convenient to the advantages such as keeping, it puts goods on the market in enormous quantities and will produce positive social benefit and significant economic benefit.
Brief description of the drawings
The present invention has 6 width accompanying drawings, wherein:
Accompanying drawing 1 structural representation of the present invention;
Accompanying drawing 2 precipitation seat outside views;
Accompanying drawing 3 is accompanying drawing 2 side-views;
Accompanying drawing 4 is accompanying drawing 3A-A-view;
Accompanying drawing 5 is collection tube structural representation;
Accompanying drawing 6 is pressurization nut structure schematic diagram.
In the drawings: 1, precipitation seat 1.1, slide glass socket 1.2, circular groove 2, collection tube 2.1, through hole 3, pressurization nut 3.1, collection tube through hole 4, rubber pad 5, filter paper 6, slide glass.
Embodiment
As shown in drawings, its structure is that described convenient body fluid cell sedimentograph comprises precipitation seat 1, collection tube 2 and pressurization nut 3 to specific embodiments of the invention; In the circular groove 1.2 on precipitation seat 1 top, rubber pad 4 is housed; Collection tube 2 is placed in groove 1.2, and pressurization nut 3 is enclosed within the outside of collection tube 2, is connected with precipitation seat 1 by screw thread.
Precipitation seat 1 is right cylinder, is processed with circular groove 1.2 on the top of precipitation seat 1, is processed with slide glass socket 1.1 in the middle and upper part of precipitation seat 1, and the bottom of slide glass socket 1.1 is higher than the inner bottom part of circular groove 1.2; Top at precipitation seat 1 is processed with outside screw; The lower external face embossing processing of precipitation seat 1.
Collection tube 2 is the cylinder table of falling T-shape, and the external diameter of its underpart round platform is slightly less than the internal diameter of circular groove 1.2; The top of top cylinder is processed with outside screw; The middle part of collection tube 2 is processed with through hole 2.1.
The inner side of pressurization nut 3 is processed with and precipitates the internal thread that is meshed of seat 1 upper external thread, is screwed tight and collection tube is fixed in circular groove 1.2 with precipitation seat 1 by the screw thread nut 3 that makes to pressurize; Top at pressurization nut 3 is processed with collection tube through hole 3.1, and the internal diameter of collection tube through hole 3.1 is slightly larger than the external diameter of collection tube top cylinder; The outside surface of pressurization nut 3 does embossing processing.
The thickness of rubber pad 4 is greater than circular groove 1.2 bottoms to the height between slide glass socket 1.1.
The using method of convenient body fluid cell sedimentograph:
A, rubber pad 4 is put in the circular groove 1.2 of precipitation seat 1;
B, by filter paper 5 folding multilayers, and perforate in the middle, the diameter in hole is identical with the diameter of the through hole 2.1 of collection tube 2; The folding size of filter paper 5 is identical with the size of slide glass 6; Filter paper 5 is placed on slide glass 6 and is together inserted in slide glass socket 1.1, make the perforate of filter paper 5 be centered close to the center of circular groove 1.2;
C, collection tube 2 is put in circular groove 1.2, and through hole 2.1 is alignd with the perforate on filter paper 5; Pressurization nut 3 is enclosed within on collection tube 2, and is threaded with precipitation seat 1, slide glass 6 and filter paper 5 are fixed on convenient body fluid cell sedimentograph;
D, the 1 milliliter of body fluid mixing is poured in the through hole 2.1 of collection tube 2, i.e. the tapping of the filter paper 5 on slide glass 6, then a cover glass is covered on the through hole 2.1 of collection tube 2, prevent that body fluid is contaminated; Moisture in body fluid is absorbed by filter paper 5, and the cell natural sedimentation in body fluid is on slide glass 6;
E, after body fluid and cellular segregation, open pressurization nut 3, take out collection tube 2, extract slide glass 6 and filter paper 5 out, separately filter paper 5 and slide glass 6, draw circle with hydrophobic pen at slide glass 6 center filter paper 5 perforate positions, select form and then the classification that after suitable dye liquor dyeing, can examine under a microscope body fluid cell according to coloured differently object.
The above; it is only preferably embodiment of the present invention; but protection scope of the present invention is not limited to this; allly be familiar with those skilled in the art in technical scope disclosed by the invention, within being equal to and replacing or change protection scope of the present invention all should be encompassed according to technical scheme of the present invention and design of the present invention thereof.
Claims (6)
1. a convenient body fluid cell sedimentograph; It is characterized in that described convenient body fluid cell sedimentograph comprises precipitation seat (1), collection tube (2) and pressurization nut (3); In the circular groove (1.2) on precipitation seat (1) top, rubber pad (4) is housed; Collection tube (2) is placed in groove (1.2), and pressurization nut (3) is enclosed within the outside of collection tube (2), is connected with precipitation seat (1) by screw thread.
2. convenient body fluid cell sedimentograph according to claim 1, it is characterized in that described precipitation seat (1) is right cylinder, top at precipitation seat (1) is processed with circular groove (1.2), middle and upper part at precipitation seat (1) is processed with slide glass socket (1.1), and the bottom of slide glass socket (1.1) is higher than the inner bottom part of circular groove (1.2); Top at precipitation seat (1) is processed with outside screw; The lower external face embossing processing of precipitation seat (1).
3. convenient body fluid cell sedimentograph according to claim 1, is characterized in that described collection tube (2) is the cylinder table of falling T-shape, and the external diameter of its underpart round platform is slightly less than the internal diameter of circular groove (1.2); The top of top cylinder is processed with outside screw; The middle part of collection tube (2) is processed with through hole (2.1).
4. convenient body fluid cell sedimentograph according to claim 1, the inner side that it is characterized in that described pressurization nut (3) is processed with and the internal thread that precipitates seat (1) upper external thread and be meshed, and is screwed tight and collection tube is fixed in circular groove (1.2) with precipitation seat (1) by the screw thread nut (3) that makes to pressurize; Top at pressurization nut (3) is processed with collection tube through hole (3.1), and the internal diameter of collection tube through hole (3.1) is slightly larger than the external diameter of collection tube top cylinder; The outside surface of pressurization nut (3) does embossing processing.
5. convenient body fluid cell sedimentograph according to claim 2, is characterized in that the thickness of described rubber pad (4) is greater than circular groove (1.2) bottom to the height between slide glass socket (1.1).
6. according to the using method of the convenient body fluid cell sedimentograph described in any one in claim 1-5:
A, rubber pad (4) is put in the circular groove (1.2) of precipitation seat (1);
B, by filter paper (5) folding multilayer, and perforate in the middle, the diameter in hole is identical with the diameter of the through hole (2.1) of collection tube (2); The folding size of filter paper (5) is identical with the size of slide glass (6); Filter paper (5) is placed on slide glass (6) and is together inserted in slide glass socket (1.1), make the perforate of filter paper (5) be centered close to the center of circular groove (1.2);
C, collection tube (2) is put in circular groove (1.2), and through hole (2.1) is alignd with the perforate on filter paper (5); It is upper that the nut (3) that will pressurize is enclosed within collection tube (2), and be threaded with precipitation seat (1), and slide glass (6) and filter paper (5) are fixed on convenient body fluid cell sedimentograph;
D, the 1 milliliter of body fluid mixing is poured in the through hole (2.1) of collection tube (2), it is the tapping of the filter paper (5) on slide glass (6), again a cover glass is covered on the through hole (2.1) of collection tube (2), prevent that body fluid is contaminated; Moisture in body fluid is absorbed by filter paper (5), and the cell natural sedimentation in body fluid is on slide glass (6);
E, after body fluid and cellular segregation, open pressurization nut (3), take out collection tube (2), extract slide glass (6) and filter paper (5) out, separately filter paper (5) and slide glass (6), draw circle at slide glass (6) center filter paper (5) perforate position with hydrophobic pen, select form and then the classification that after suitable dye liquor dyeing, can examine under a microscope body fluid cell according to coloured differently object.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310027919.5A CN103103107B (en) | 2013-01-24 | 2013-01-24 | Convenient body fluid cell precipitation instrument and using method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310027919.5A CN103103107B (en) | 2013-01-24 | 2013-01-24 | Convenient body fluid cell precipitation instrument and using method thereof |
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| Publication Number | Publication Date |
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| CN103103107A CN103103107A (en) | 2013-05-15 |
| CN103103107B true CN103103107B (en) | 2014-11-05 |
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| CN201310027919.5A Expired - Fee Related CN103103107B (en) | 2013-01-24 | 2013-01-24 | Convenient body fluid cell precipitation instrument and using method thereof |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105482990A (en) * | 2016-01-22 | 2016-04-13 | 王翠玲 | Composite body fluid cell precipitation instrument |
| CN106318856A (en) * | 2016-08-11 | 2017-01-11 | 马杰 | Cell enrichment device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2171407Y (en) * | 1993-07-07 | 1994-07-13 | 中国人民解放军第四军医大学 | Assembly test tube |
| CN201344877Y (en) * | 2009-02-04 | 2009-11-11 | 中国人民解放军第四军医大学第一附属医院 | Cell centrifugation sediment tube |
| CN102539211A (en) * | 2012-01-09 | 2012-07-04 | 广州三瑞医疗器械有限公司 | Device and method for making liquid-based cytological smear in one step |
| CN203079955U (en) * | 2013-01-24 | 2013-07-24 | 大连医科大学附属第一医院 | Convenient body fluid cell precipitating instrument |
-
2013
- 2013-01-24 CN CN201310027919.5A patent/CN103103107B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2171407Y (en) * | 1993-07-07 | 1994-07-13 | 中国人民解放军第四军医大学 | Assembly test tube |
| CN201344877Y (en) * | 2009-02-04 | 2009-11-11 | 中国人民解放军第四军医大学第一附属医院 | Cell centrifugation sediment tube |
| CN102539211A (en) * | 2012-01-09 | 2012-07-04 | 广州三瑞医疗器械有限公司 | Device and method for making liquid-based cytological smear in one step |
| CN203079955U (en) * | 2013-01-24 | 2013-07-24 | 大连医科大学附属第一医院 | Convenient body fluid cell precipitating instrument |
Non-Patent Citations (2)
| Title |
|---|
| 介绍一种自制简易脑脊液细胞沉淀器;李次芬 李瑞友 叶义德;《中国人民解放军军医进修学院学报》;19841230;第5卷(第4期);第318页 * |
| 李次芬 李瑞友 叶义德.介绍一种自制简易脑脊液细胞沉淀器.《中国人民解放军军医进修学院学报》.1984,第5卷(第4期),第318页. * |
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