CN103100114A - Preparation method of medical metal surface slow-released growth factor coating - Google Patents
Preparation method of medical metal surface slow-released growth factor coating Download PDFInfo
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- CN103100114A CN103100114A CN2013100245165A CN201310024516A CN103100114A CN 103100114 A CN103100114 A CN 103100114A CN 2013100245165 A CN2013100245165 A CN 2013100245165A CN 201310024516 A CN201310024516 A CN 201310024516A CN 103100114 A CN103100114 A CN 103100114A
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Abstract
The invention discloses a preparation method of a medical metal surface slow-released growth factor coating. The method comprises the steps of firstly, using graphene oxide as the growth factor carrier, wrapping growth factors in composite nano granules formed by polycations and polyanions, reacting the nano granules containing the growth factors with the carboxylated graphene oxide so as to form amido bonds, thereby fixing the nano granules on the graphene oxide; and enabling the graphene oxide carrying the growth factors to form the coating on a biologic medical metal surface which is subjected to dopamine treatment by using an extraction method. By utilizing the preparation method, higher growth factor carrying quantity is realized, and meanwhile, the activity of the factors is protected, and the slow-releasing of the factors is realized.
Description
Technical field
The present invention relates to the preparation method of the slow release somatomedin coating on a kind of medical metal surface.
Background technology
Medical metal is widely used in the fields such as surgical plastic, bone implant due to its good biocompatibility and good mechanical property.But medical metal is all biologically inert usually, and a little less than its surperficial bone regeneration capability, new bone growth is slow.After this causes medical metal to implant, and can not form fast firmly bonding between the surrounding bone tissue.In order to improve the integration ability between medical metal implant and osseous tissue, often need it is carried out surface modification.Wherein a kind of method of surface modification is to be written at the medical metal implant surfaces regeneration that cell growth factor promotes osseous tissue.Common cell growth factor plays a part crucial in the bone repair process as bone morphogenetic protein (BMP-2), angiogenesis factor (VEGF), conversion growth factor (TGF-β 2) etc.The method of loading at present somatomedin has directly somatomedin is adsorbed on material surface, but this method can't realize the slow releasing function of somatomedin, and somatomedin is exposed to the impact that is easy to be subjected to various factors in physiological environment and inactivation; Also have the biomedical metallic material after processing is combined in the mode of covalent bond with somatomedin, though this mode can realize the immobilized of somatomedin, but the covalent bonds mode tends to cause the somatomedin inactivation.
Summary of the invention
The preparation method that the purpose of this invention is to provide the slow release somatomedin coating on a kind of medical metal surface, the somatomedin load capacity of the slow release somatomedin coating that this legal system is standby is high, somatomedin can slowly discharge with body in the new bone growth speed adaption, and somatomedin is active strong.
The present invention realizes that the technical scheme that its goal of the invention adopts is, the preparation method of the surperficial slow release somatomedin of a kind of medical metal coating, and its concrete steps are as follows:
The parcel of A, somatomedin:
To have the somatomedin of facilitation to be dissolved in the polyanion solution that concentration is 0.5-2.5mg/ml to osteogenesis, and obtain containing the polyanion solution of somatomedin, the concentration of somatomedin be 0.5-10 μ g/ml.
With the described said polycation solution that contains polyanion solution and the band amino that concentration is 0.5-2.5mg/ml of somatomedin, by volume 1: the ratio of 2-5 is mixed, and stirring, centrifugal, lyophilization, obtains being loaded with the nano-particle of somatomedin;
B, graphene oxide carboxylated:
Be the ClCH of 20mg/ml with concentration
2COONa solution and concentration are that the NaOH solution of 20mg/ml was mixed with mixed solution in 1: 1 by volume, then the graphene oxide of 80mg are added in the mixed solution of 4-6ml and react 1.5-2.5h, make the oxy radical on graphene oxide carboxylated; Then with solution dialysis and lyophilizing, obtain carboxylated graphene oxide powder.
C, nano-particle immobilized:
The nano-particle that carries somatomedin that the carboxylated graphene oxide powder that B step is obtained and A step make carries out the EDC/NHS catalytic reaction, makes the nano-particle of amino formation amido link, year somatomedin of the carboxyl on carboxylated graphene oxide surface and nano grain surface immobilized on graphene oxide; Then with reactant liquor dialysis, lyophilization, then dry parts is dissolved in the buffer solution of pH value 6.0, forms graphene oxide/nanoparticles solution;
D, medical metal material lixiviate in the C graphene oxide/nanoparticles solution in step that will be grafted with the dopamine coating make graphite oxide ene coatings that load has a somatomedin be combined in the medical metal material surface by Electrostatic Absorption.
Compared with prior art, the invention has the beneficial effects as follows:
One, compare with the direct somatomedin that loads on substrate material surface, the present invention first is wrapped in somatomedin in nano-particle and is combined with graphene oxide, load in the process of material surface at somatomedin, only depending on the electrostatic interaction of somatomedin between polyelectrolyte ion is combined, do not relate to chemical reaction, the activity of somatomedin can not be damaged, and can keep well the activity of somatomedin.
Two, graphene oxide is that the surface is with the Graphene derivant of multiple oxy radical.It has monoatomic layer network structure structure, and specific surface area is very large, can will be loaded with the nano-particle of somatomedin wrap up and load capacity large, can realize the high-efficient carrier of medicine, and can regulate and control preferably the slow release of somatomedin.
Above-mentioned medical metal is titanium, titanium alloy or rustless steel.
Above-mentioned somatomedin is BMP-2, VEGF or TGF-β 2.These somatomedin have obvious facilitation to osteogenesis.
Above-mentioned polycation is chitosan or polylysine, and polyanion is heparin, chondroitin sulfate, sodium alginate or hyaluronic acid.
The invention will be further described below in conjunction with the specific embodiment.
The specific embodiment
Embodiment one
The preparation method of a kind of medical metal surface slow release somatomedin coating, its concrete steps are as follows:
The parcel of A, somatomedin:
To there be the growth factor B MP-2 of facilitation to be dissolved in the chondroitin sulfate cellulose solution that concentration is 0.5mg/ml to osteogenesis, obtain containing the chondroitin sulfate cellulose solution of BMP-2, and the concentration of BMP-2 in the chondroitin sulfate cellulose solution be 0.5 μ g/ml;
With the described chondroitin sulfate cellulose solution of growth factor B MP-2 and the polylysine solution of 0.5mg/ml of containing, mix by the volume ratio of 1: 2, and stirring, centrifugal, lyophilization, obtain being loaded with the nano-particle of BMP-2;
B, graphene oxide carboxylated:
Be the ClCH of 20mg/ml with concentration
2COONa solution and concentration are that the NaOH80mg solution of 20mg/ml is to be mixed with mixed solution at 1: 1 by volume, then the 80mg graphene oxide is added in the 6ml mixed solution react 2.5h, make the oxy radical on graphene oxide carboxylated; Then with solution dialysis and lyophilizing, obtain carboxylated graphene oxide powder;
C, nano-particle immobilized:
The nano-particle that carries somatomedin that the carboxylated graphene oxide powder that B step is obtained and A step make carries out the EDC/NHS catalytic reaction, makes the nano-particle of amino formation amido link, year somatomedin of the carboxyl on carboxylated graphene oxide surface and nano grain surface immobilized on graphene oxide; Then with reactant liquor dialysis, lyophilization, then dry parts is dissolved in the buffer solution of pH value 6.0, forms graphene oxide/nanoparticles solution;
Wherein, the EDC/NHS catalytic reaction is prior art, and its concrete operations can be:
C1, the carboxylated graphene oxide of B step preparation is dissolved in MES (MES) (0.1M) in solution, obtaining concentration is the MES solution of the carboxylated graphene oxide of 1mg/ml; Adding 0.4mg concentration in the MES solution of the carboxylated graphene oxide of 1ml is that EDC (1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides) and the 0.6mg concentration of 2mM is the NHS (N-hydroxy-succinamide) of 5mM, and pH is transferred to 5～6; React 15min under room temperature; Adding 1.4 μ l concentration in the solution is the unnecessary EDC of 2 mercapto ethanol (2-mercapto ethanol) neutralization of 20mM again;
C2, with the nano-particle that is loaded with growth factor B MP-2 of A step preparation, evenly be dissolved in phosphate buffered solution (PBS), obtaining concentrations of nanoparticles is the phosphate buffered solution solution of 1mg/ml, getting this solution of 1ml adds in C1 resulting solution of step, regulate pH to 7.2, react 2h under room temperature.Adding at last concentration is the azanol (10mM) of the 10mM unreacted NHS that neutralizes.
D, titanium alloy lixiviate in the C graphene oxide/nanoparticles solution in step that will be grafted with the dopamine coating, electronegative load has the graphene oxide of nano-particle and the dopamine coating of positively charged to pass through Electrostatic Absorption, thereby makes load have the graphene oxide of nano-particle to be combined in titanium alloy surface.
Embodiment two
The preparation method of a kind of medical metal surface slow release somatomedin coating, its concrete steps are as follows:
The parcel of A, somatomedin:
To have the growth factor VEGF of facilitation to be dissolved in the heparin solution that concentration is 1.5mg/ml to osteogenesis, and obtain containing the heparin solution of VEGF, and in heparin solution, the concentration of VEGF be 5 μ g/ml;
With the described heparin solution of growth factor VEGF and the chitosan solution of 2.5mg/ml of containing, mix by the volume ratio of 1: 4, and stirring, centrifugal, lyophilization, obtain being loaded with the nano-particle of BMP-2;
B, graphene oxide carboxylated:
Be the ClCH of 20mg/ml with concentration
2COONa solution and concentration are that the NaOH80mg solution of 20mg/ml is to be mixed with mixed solution at 1: 1 by volume, then the 80mg graphene oxide is added in the 5ml mixed solution react 2h, make the oxy radical on graphene oxide carboxylated; Then with solution dialysis and lyophilizing, obtain carboxylated graphene oxide powder;
C, nano-particle immobilized:
The nano-particle that carries somatomedin that the carboxylated graphene oxide powder that B step is obtained and A step make carries out the EDC/NHS catalytic reaction, makes the nano-particle of amino formation amido link, year somatomedin of the carboxyl on carboxylated graphene oxide surface and nano grain surface immobilized on graphene oxide; Then with reactant liquor dialysis, lyophilization, then dry parts is dissolved in the buffer solution of pH value 6.0, forms graphene oxide/nanoparticles solution;
Wherein, the EDC/NHS catalytic reaction is prior art, and its concrete operations can be:
C1, the carboxylated graphene oxide of B step preparation is dissolved in MES (MES) (0.1M) in solution, obtaining concentration is the MES solution of the carboxylated graphene oxide of 1mg/ml; Adding 0.4mg concentration in the MES solution of the carboxylated graphene oxide of 1ml is that EDC (1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides) and the 0.6mg concentration of 2mM is the NHS (N-hydroxy-succinamide) of 5mM, and pH is transferred to 5; React 15min under room temperature; Adding 1.4 μ l concentration in the solution is the unnecessary EDC of 2 mercapto ethanol (2-mercapto ethanol) neutralization of 20mM again;
C2, with the nano-particle that is loaded with growth factor VEGF of A step preparation, evenly be dissolved in phosphate buffered solution (PBS), obtaining concentrations of nanoparticles is the phosphate buffered solution solution of 1mg/ml, getting this solution of 1ml adds in C1 resulting solution of step, regulate pH to 7.2, react 2h under room temperature.Adding at last concentration is the azanol (10mM) of the 10mM unreacted NHS that neutralizes.
D, titanium lixiviate in the C graphene oxide/nanoparticles solution in step that will be grafted with the dopamine coating, electronegative load has the dopamine coating of the graphene oxide of nano-particle and positively charged by Electrostatic Absorption, thereby makes load have the graphene oxide of nano-particle to be combined in the titanium surface
Embodiment three
The preparation method of a kind of medical metal surface slow release somatomedin coating, its concrete steps are as follows:
The parcel of A, somatomedin:
To there be the somatomedin TGF-β 2 of facilitation to be dissolved in the sodium alginate solution that concentration is 2.5mg/ml to osteogenesis, obtain containing the sodium alginate soln of TGF-β 2, and the concentration of TGF-β 2 be 10 μ g/ml;
With the described sodium alginate soln of somatomedin TGF-β 2 and the chitosan solution of 1.0mg/ml of containing, mix by the volume ratio of 1: 5, and stirring, centrifugal, lyophilization, obtain being loaded with the nano-particle of BMP-2;
B, graphene oxide carboxylated:
Be the ClCH of 20mg/ml with concentration
2COONa solution and concentration are that the NaOH80mg solution of 20mg/ml is to be mixed with mixed solution at 1: 1 by volume, then the 80mg graphene oxide is added in the 4ml mixed solution react 1.5h, make the oxy radical on graphene oxide carboxylated; With solution dialysis and lyophilizing, obtain carboxylated graphene oxide powder after reaction;
C, nano-particle immobilized:
The nano-particle that carries somatomedin that the carboxylated graphene oxide powder that B step is obtained and A step make carries out the EDC/NHS catalytic reaction, makes the nano-particle of amino formation amido link, year somatomedin of the carboxyl on carboxylated graphene oxide surface and nano grain surface immobilized on graphene oxide; Then with reactant liquor dialysis, lyophilization, then dry parts is dissolved in the buffer solution of pH value 6.0, forms graphene oxide/nanoparticles solution;
Wherein, the EDC/NHS catalytic reaction is prior art, and its concrete operations can be:
C1, the carboxylated graphene oxide of B step preparation is dissolved in MES (MES) (0.1M) in solution, obtaining concentration is the MES solution of the carboxylated graphene oxide of 1mg/ml; Adding 0.4mg concentration in the MES solution of the carboxylated graphene oxide of 1ml is that EDC (1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides 1-ethyl-3-(3-dimethyl aminopropyl)-carbodiimides) and the 0.6mg concentration of 2mM is the NHS (N-hydroxy-succinamide) of 5mM, and pH is transferred to 6; React 15min under room temperature; Adding 1.4 μ l concentration in the solution is the unnecessary EDC of 2 mercapto ethanol (2-mercapto ethanol) neutralization of 20mM again;
C2, with the nano-particle that is loaded with somatomedin TGF-β 2 of A step preparation, evenly be dissolved in phosphate buffered solution (PBS), obtaining concentrations of nanoparticles is the phosphate buffered solution solution of 1mg/ml, getting this solution of 1ml adds in C1 resulting solution of step, regulate pH to 7.5, react 2h under room temperature.Adding at last concentration is the azanol (10mM) of the 10mM unreacted NHS that neutralizes.
D, rustless steel lixiviate in the C graphene oxide/nanoparticles solution in step that will be grafted with the dopamine coating, electronegative load has the graphene oxide of nano-particle and the dopamine coating of positively charged to pass through Electrostatic Absorption, thereby makes load have the graphene oxide of nano-particle to be combined in stainless steel surfaces.
Embodiment four
The operation of this example and embodiment one are basic identical, and different is only: change the polyanion of preparation nano-particle in example one into hyaluronic acid by chondroitin sulfate.
The medical metal material that is grafted with the dopamine coating that uses in the present invention can adopt prior art to make, and makes as adopting following methods:
The dopamine of 2mg/ml is dissolved in regulates pH to 8.5 in Tris-hydrochloric acid (tris-HCl) solution of 10mM, again the medical metal material after pickling, alkali cleaning is dipped into 12h in solution, then rinse medical metal material three times, just dopamine coating on medical metal material (titanium, titanium alloy, rustless steel) surface grafting.
Claims (4)
1. the preparation method of medical metal surface slow release somatomedin coating, its concrete steps are as follows:
The parcel of A, somatomedin:
To have the somatomedin of facilitation to be dissolved in the polyanion solution that concentration is 0.5-2.5mg/ml to osteogenesis, and obtain containing the polyanion solution of somatomedin, the concentration of somatomedin be 0.5-10 μ g/ml.
With the described said polycation solution that contains polyanion solution and the band amino that concentration is 0.5-2.5mg/ml of somatomedin, by volume 1: the ratio of 2-5 is mixed, and stirring, centrifugal, lyophilization, obtains being loaded with the nano-particle of somatomedin;
B, graphene oxide carboxylated:
Be the ClCH of 20mg/ml with concentration
2COONa solution and concentration are that the NaOH solution of 20mg/ml was mixed with mixed solution in 1: 1 by volume, then the graphene oxide of 80mg are added in the mixed solution of 4-6ml and react 1.5-2.5h, make the oxy radical on graphene oxide carboxylated; Then with solution dialysis and lyophilizing, obtain carboxylated graphene oxide powder.
C, nano-particle immobilized:
The nano-particle that carries somatomedin that the carboxylated graphene oxide powder that B step is obtained and A step make carries out the EDC/NHS catalytic reaction, makes the nano-particle of amino formation amido link, year somatomedin of the carboxyl on carboxylated graphene oxide surface and nano grain surface immobilized on graphene oxide; Then with reactant liquor dialysis, lyophilization, then dry parts is dissolved in the buffer solution of pH value 6.0, forms graphene oxide/nanoparticles solution;
D, medical metal material lixiviate in the C graphene oxide/nanoparticles solution in step that will be grafted with the dopamine coating make graphite oxide ene coatings that load has a somatomedin be combined in the medical metal material surface by Electrostatic Absorption.
2. the preparation method of a kind of medical metal according to claim 1 surface slow release somatomedin coating, it is characterized in that: described medical metal is titanium, titanium alloy or rustless steel.
3. the preparation method of a kind of medical metal according to claim 1 surface slow release somatomedin coating, it is characterized in that: described somatomedin is BMP-2, VEGF or TGF-β 2.
4. the preparation method of a kind of medical metal according to claim 1 surface slow release somatomedin coating, it is characterized in that: described polycation is chitosan or polylysine, and described polyanion is chondroitin sulfate, heparin, sodium alginate or hyaluronic acid.
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|---|---|---|---|---|
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| CN104141124A (en) * | 2014-06-17 | 2014-11-12 | 天津大学 | Method for improving biological activity of pure titanium surface by using dopamine to be bonded with graphene oxide |
| CN104531669A (en) * | 2014-12-30 | 2015-04-22 | 苏州英芮诚生化科技有限公司 | Magnetic graphene nanocomposite material cladded with hydrophilic polydopamine and preparing method and application thereof |
| CN105063423A (en) * | 2015-08-06 | 2015-11-18 | 陆春玲 | Medical graphene enhanced titanium alloy rod |
| CN105063424A (en) * | 2015-08-06 | 2015-11-18 | 陆春玲 | Preparation method of medical titanium alloy bar |
| CN105063421A (en) * | 2015-08-06 | 2015-11-18 | 陆春玲 | Preparation method of medical graphene enhanced titanium alloy rod with high antibacterial ability |
| CN105063420A (en) * | 2015-08-06 | 2015-11-18 | 陆春玲 | Preparation method for medical titanium alloy bar with strong antibacterial ability |
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| CN113842501A (en) * | 2021-10-21 | 2021-12-28 | 华中科技大学同济医学院附属协和医院 | Surface modification material for internal implant and preparation method and application thereof |
| CN114601962A (en) * | 2020-12-09 | 2022-06-10 | 上海交通大学 | Polydopamine-graphene composite coating on surface of biomedical magnesium alloy and preparation thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1394901A (en) * | 2002-07-10 | 2003-02-05 | 浙江大学 | Biological material using electrostatic attraction layer-layer self-assembled modified polyester material as surface with cell compatibility |
| CN1791437A (en) * | 2003-05-16 | 2006-06-21 | 布卢薄膜有限责任公司 | Medical implants comprising biocompatible coatings |
| WO2009085015A1 (en) * | 2008-01-03 | 2009-07-09 | National University Of Singapore | Functionalised graphene oxide |
| CN101721389A (en) * | 2009-11-30 | 2010-06-09 | 浙江大学 | Method for preparing in-situ therapeutic substance-loaded microcapsules |
| CN102000658A (en) * | 2010-12-15 | 2011-04-06 | 西南交通大学 | Polydopamine-based biofunction modification method |
| CN102433032A (en) * | 2011-09-08 | 2012-05-02 | 南京师范大学 | Controllable method for synthesizing carboxylic graphene oxide and prepared nano material |
| CN102677032A (en) * | 2012-05-24 | 2012-09-19 | 西南交通大学 | Method for immobilizing VEGF-carried heparin/polylysine nanoparticles on Ti surface |
-
2013
- 2013-01-23 CN CN201310024516.5A patent/CN103100114B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1394901A (en) * | 2002-07-10 | 2003-02-05 | 浙江大学 | Biological material using electrostatic attraction layer-layer self-assembled modified polyester material as surface with cell compatibility |
| CN1791437A (en) * | 2003-05-16 | 2006-06-21 | 布卢薄膜有限责任公司 | Medical implants comprising biocompatible coatings |
| WO2009085015A1 (en) * | 2008-01-03 | 2009-07-09 | National University Of Singapore | Functionalised graphene oxide |
| CN101721389A (en) * | 2009-11-30 | 2010-06-09 | 浙江大学 | Method for preparing in-situ therapeutic substance-loaded microcapsules |
| CN102000658A (en) * | 2010-12-15 | 2011-04-06 | 西南交通大学 | Polydopamine-based biofunction modification method |
| CN102433032A (en) * | 2011-09-08 | 2012-05-02 | 南京师范大学 | Controllable method for synthesizing carboxylic graphene oxide and prepared nano material |
| CN102677032A (en) * | 2012-05-24 | 2012-09-19 | 西南交通大学 | Method for immobilizing VEGF-carried heparin/polylysine nanoparticles on Ti surface |
Non-Patent Citations (6)
| Title |
|---|
| CHYE KHOON POH ET AL.: "The effect of VEGF functionalization of titanium on endothelial cells in vitro", 《BIOMATERIALS》 * |
| MEHMET OZSOZ ET AL.: "Electrochemical Genosensor Based on Colloidal Gold Nanoparticles for the Detection of Factor V Leiden Mutation Using Disposable Pencil Graphite Electrodes", 《ANALYTICAL CHEMISTRY》 * |
| MIN HUANG ET AL.: "Polyelectrolyte Complexes Stabilize and Controllably Release Vascular Endothelial Growth Factor", 《BIOMACROMOLECULES》 * |
| VIRENDRA SINGH ET AL.: "Grapheme based materials: past, present and future", 《PROGRESS IN MATERIALS SCIENCE》 * |
| WONG CHENG LEE ET AL.: "Origin of Enhanced Stem Cell Growth and Differentiation on Graphene and Graphene Oxide", 《ACS NANO》 * |
| 徐东等: "羧基化氧化石墨烯的血液相容性", 《高等学校化学学报》 * |
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