CN106963982A - A kind of repair materials adhered to anti-bacteria wet keeping appropriate tissue and its preparation method and application - Google Patents
A kind of repair materials adhered to anti-bacteria wet keeping appropriate tissue and its preparation method and application Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/34—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/18—Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment
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Abstract
The present invention relates to a kind of repair materials adhered to anti-bacteria wet keeping appropriate tissue and its preparation method and application, the preparation method of the repair materials is as follows：S1. sulfhydrylation modification is carried out to hyaluronic acid；S2. chitosan is carried out fully washing after maleylation modification S3. soaks nanometer spinning fibre through dopamine solution, then nanometer spinning fibre is immersed in thiolated hyaluronic acid solution and Nmaleoyl chitosan solution successively and adsorbed, elution after having adsorbed；S4. the repair materials that absorption, elution step produce thiolated hyaluronic acid and Nmaleoyl chitosan alternating sorbent afterwards several times are repeated.The repair materials that the present invention is provided have good antibacterial and performance of keeping humidity, and with preferable tissue adherence；It can be as water soluble drug and bioactie agent stabilization, high-efficiency sustained-release carrier, it is adaptable to organizational project application field especially skin repair field.
Description
Technical field
The present invention relates to technical field of biological materials, and in particular to a kind of reparation adhered to anti-bacteria wet keeping appropriate tissue
Material and its preparation method and application.
Background technology
Preferable tissue renovation material should be able to farthest analog cell epimatrix 26S Proteasome Structure and Function, be provided simultaneously with storage
And the ability of the sustained-release activity factor, so that instruct and promote cell behavior, inducing tissue regeneration.Therefore, study and design " structure
And function bionicses " repair materials be the development of current biomedical material important directions.The nanometer obtained by electrostatic spinning
Spinning fibre has larger specific surface area, regulatable porosity and preferable ductility, and can simulate outside n cell
Matrix structure and function, can be widely applied to the repair medical sciences such as artificial blood vessel, skin ultrastructure and bone tissue engineer
Various aspects.Natural and synthesis high polymer material, ceramic material etc. are used equally for the preparation of electrospun fibers, wherein, people
Work synthesizes high polymer material because its excellent mechanical property and workability are applied in the research of electrostatic spinning repair materials
It is the most extensive.Lived however, spinning nanofiber prepared by such synthesis high polymer material often possesses poor hydrophily, biology
Sex factor adsorptivity, tissue adherence and cellular affinity.
Artificial synthesized high polymer material and natural macromolecular material are subjected to composite spinning or such as grown with active factors because
It is the important means currently used for the artificial synthesized high molecular nanometer spinning fibre performance of improvement that son, medicine etc., which carry out cospinning etc.,.Will
Natural polymer and artificial synthesized high polymer material carry out composite spinning, are to confer to the good mechanics of spinning fibre and biology performance
Important means, but this method generally requires to find suitable cosolvent, and this greatly limits the range of choice of spinning material.
Realize that though the load of active factors can improve the biological activity of material to a certain extent using co spun technology, but spinning work
Skill condition has certain influence on the activity of load factor, and also can high degree to the demand of special solvent based on high polymer material
The range of choice of the restricted activity factor.Layer-by-layer is a kind of can be used for the important of repair materials progress surface modification
Technological means, its reaction condition is gentle, simple to operate, economy, and Iy self-assembled layer can inherently be used as bioactie agent and medicine
The natural reservoirs of thing.However, though LBL self-assembly decorative layer is with good biological activity, it is under physiological environment
Stability is often inadequate, it is also difficult to the real effect realized to load factor or medicament slow release.
Therefore, still need to study that a kind of biological activity is good, 26S Proteasome Structure and Function is bionical, and being capable of carrying medicament or bioactivity
The factor simultaneously realizes that it is sustained, also the tissue renovation material with better stability.
The content of the invention
Have what anti-bacteria wet keeping appropriate tissue was adhered to it is an object of the invention to overcome the deficiencies of the prior art and provide a kind of
Repair materials, the repair materials that provide of the present invention have good antibacterial and performance of keeping humidity, and with preferable tissue adherence；
It can be as water soluble drug and bioactie agent stabilization, high-efficiency sustained-release carrier, it is adaptable to organizational project application field is outstanding
It is skin repair field.
Another object of the present invention is to provide the above-mentioned repair materials adhered to anti-bacteria wet keeping appropriate tissue as load
Application of the body in water soluble drug and bioactie agent load.
Another object of the present invention is to provide the above-mentioned repair materials adhered to anti-bacteria wet keeping appropriate tissue in tissue
Application in engineering field.
To achieve the above object, the present invention is adopted the following technical scheme that：
A kind of repair materials adhered to anti-bacteria wet keeping appropriate tissue, the preparation method of the repair materials is as follows：
S1：Sulfhydrylation modification is carried out to hyaluronic acid solution and controls free sulfhydryl groups content to be 100~200 μm of olg-1, warp
Dialysis, freeze-drying produce thiolated hyaluronic acid；
S2：Maleylation modification is carried out to chitosan solution and controls acetyl content to be 1500~2000 μm of olg-1, warp
Dialysis, freeze-drying produce Nmaleoyl chitosan；
S3. fully washed after nanometer spinning fibre is soaked through dopamine solution, nanometer spinning fibre is then immersed into mercapto successively
Adsorbed in base hyaluronic acid solution and Nmaleoyl chitosan solution, elution after having adsorbed；
S4：Repeat absorption, elution step and produce thiolated hyaluronic acid and Nmaleoyl chitosan alternating sorbent afterwards several times
Repair materials；
Wherein, the concentration of thiolated hyaluronic acid solution is 0.25~0.75 mgmL-1, the Nmaleoyl chitosan solution
Concentration be 0.25~0.75 mgmL-1。
The nanometer spinning fibre prepared by electrostatic spinning technique have larger specific surface area, regulatable porosity and
Preferable ductility, adsorptivity, and the advantages such as natural extracellular matrix 26S Proteasome Structure and Function can be simulated, become repair materials development
New direction, but simple artificial synthesized high molecular nanometer spinning fibre does not often have antibacterial, moisture retention.Need to enter it
Row appropriate modification and modification, are used for the feasibility of wound repair to improve it.With being repaiied by chitosan with hyaluronic acid merely
Decorations are compared, and the present invention is used it for assign after spinning fibre modification and repaiied by the chemical modification to chitosan and hyaluronic acid
The good antibacterial ability of multiple material, without carrying out extra antibacterials load.
Although the self-assembled modified layer of natural polymer is with good biological activity, but it is steady under physiological environment
It is qualitative often inadequate, its stability need to be improved by chemically or physically means.The present invention passes through poly- to hyaluronic acid and shell
Sugar is chemically modified, and in the intermolecular sulfydryl and double bond introduced with reactivity, it is carried out to nanometer spinning fibre
Intramolecular crosslinking can be formed in situ during self-assembled modified, the effect of the sour and chitosan-modified layer of stable transparent matter is reached.
Simultaneously, it is to avoid the use of small-molecule chemical crosslinking agent afterwards, so as to reduce a series of steps such as follow-up small molecules cleaning,
Also the genotoxic potential effect of small-molecule chemical crosslinking agent presence has been evaded to a certain extent.
The present invention is modified by the modification of self assembly biological functional to nanometer spinning fibre, is maintaining nanometer spinning fine
Its biological function is improved on the basis of dimension matrix material superperformance, there is good resist through the repair materials obtained by modification
Bacterium and performance of keeping humidity, its retentiveness have also obtained obvious improvement, also with preferable tissue adherence.In addition, the present invention is carried
Nanometer spinning fibre after the biological functional modification of confession can bear the extraneous ring such as pH, high ionic strength and mechanical force
Border pressure, can be applied in biomedical tissue engineering field as pharmaceutical carrier, tissue engineering bracket or wound repair materials.
The inventive method is modified spinning fibre using layer-by-layer, and reaction medium is the aqueous solution, modification
Process is carried out at room temperature, and the load of subsequent bio active factors or medicine is also to carry out on this condition, will not cause material
Degraded and denaturation, do not interfere with the biological activity of load factor yet.
Preferably, the free sulfhydryl groups content is 100~200 μm of olg-1。
Preferably, the acetyl content is 1500~2000 μm of olg-1。
Preferably, the concentration of thiolated hyaluronic acid solution is 0.5 mgmL-1, the Nmaleoyl chitosan solution
Concentration is 0.5 mgmL-1。
Preferably, absorption, elution step are repeated in S4 9~11 times.
Preferably, 1- ethyls -3- (3- DimethylAminopropyls)-carbodiimide hydrochlorides and N- hydroxysuccinimidyls are selected in S1
Acid imide carries out activation process to hyaluronic acid aqueous solution；Sulfhydrylation is carried out from cysteine hydrochloride to hyaluronic acid to repair
Decorations.
Preferably, the mol ratio of chitosan and maleic anhydride is 0.5~2 in S2:1.
Preferably, the pH of thiolated hyaluronic acid solution is 5~7 in S3, and the pH of Nmaleoyl chitosan solution is 3~5.
The above-mentioned repair materials adhered to anti-bacteria wet keeping appropriate tissue are as carrier in water soluble drug or bioactivity
Application in factor loads is also within protection scope of the present invention.
Application of the above-mentioned repair materials with the adhesion of anti-bacteria wet keeping appropriate tissue in field of tissue engineering technology is also in this hair
Within bright protection domain.
Preferably, application of the repair materials in skin repair field.
In the present invention, the decorative layer mainly realizes it on spinning fibre surface by the reaction of functional group between macromolecule
Self assembly.
In the present invention, the nanometer spinning fibre in the S3 can be polylactic acid nano spinning fibre, can also basis
Repair medical science domain requirement is fine from other high molecular nanometer spinning fibres such as polylactic-co-glycolic acid nanometer spinning of this area
Dimension etc..
Compared with prior art, the present invention has the advantages that：
The present invention by existing nanometer spinning fibre carry out biological functional modification there is provided one kind have good antibacterial and
The repair materials of performance of keeping humidity, the repair materials also have preferable tissue adherence.The repair materials that the present invention is provided can be made
For the stabilization of water soluble drug or bioactie agent, high-efficiency sustained-release carrier, it is adaptable to organizational project application field especially skin
Skin repairs field.In addition, the present invention is used for method and system easily operation and the control, experiment condition temperature for preparing repair materials
It is with, cost low, it is to avoid the input of high-energy source, with larger application value.
Brief description of the drawings
Fig. 1 is the hyaluronic acid and the nuclear magnetic spectrogram of chitosan after before modified；
Fig. 2 is the appearance structure of polylactic acid nano spinning fibre under the conditions of different modifying；
Fig. 3 is the hydrophilic and hydrophobic figure of self-assembled modified front and rear polylactic acid nano spinning fibre；
Fig. 4 is the antibacterial ability figure of self-assembled modified front and rear polylactic acid nano spinning fibre；
Fig. 5 can try hard to for the tissue adhension of self-assembled modified front and rear polylactic acid nano spinning fibre（*p<0.05）；
Fig. 6 is the sustained release behavior of insulin in self-assembled modified front and rear polylactic acid nano spinning fibre；
Fig. 7 is that different spinning fibre surfaces umbilical cord mesenchymal stem cells breed behavior（*p<0.05）.
Embodiment
With reference to example, the present invention is described further, and the given examples are served only to explain the present invention, but the present invention
Claimed scope is not limited to the scope that example is stated.
A kind of repair materials adhered to anti-bacteria wet keeping appropriate tissue of embodiment 1
（1）The preparation of polylactic acid nano spinning fibre
7 wt% polylactic acid powder is dissolved in DCM/DMF mixed liquors and is fitted into 20 mL syringes and is fixed on micro note
Penetrate on pump, using capillary of the syringe needle scabbled as injection thread, capillary inner diameter is 0.4 mm, electric-field intensity 0.8
kv·cm-1, receive the cm of distance 12, the mLh of extrusion capacity 1-1.The fiber that spinning is obtained is collected on rotating cylinder, and vacuum is done
The dry organic solvent for removing residual can obtain polylactic acid nano spinning fibre.
（2）Polylactic acid nano spinning fibre it is self-assembled modified
1. hyaluronic acid is thiolated modified
Prepare hyaluronic acid（HA）The aqueous solution, sequentially adds final concentration of 50 mmolL-11- ethyls -3- (3- dimethyl amines
Propyl group)-carbodiimide hydrochloride（EDAC）And n-hydroxysuccinimide（NHS）, regulation system pH is about 5~6.Lucifuge,
It is stirred at room temperature after reaction certain time, a certain amount of cysteine hydrochloride is added in reaction system, controls free sulfhydryl groups content
In 160 μm of olg-1Left and right, regulation system pH is about 5, lucifuge, be stirred at room temperature reaction 5 h after, dialyse 3 days, after freeze-drying
Sulfhydrylation HA can be obtained（tHA）.
2. the maleylation of chitosan is modified
By the chitosan for 1 wt% being completely dissolved（Chi）Solution is placed in three-necked flask, according to Chi repeat units and maleic acid
Acid anhydride mol ratio is 1:1 rate of charge adds maleic anhydride into chitosan solution, and it is 1971 μm of olg to control acetyl group number-1
Left and right, dialyses 3 days after 24 h of lucifuge reaction, to remove impurity and unreacted small molecule, can be obtained after freeze-drying at room temperature
Maleylation Chi（MA-Chi）.
3. the configuration of complex polyelectrolyte particle solution
Each polyelectrolyte solution is formulated as follows：
Dopamine（2 mg·mL-1）It is dissolved in Tris-HCl（PH is 8.5）In solution；
HA after sulfhydrylation is modified（0.5 mg·mL-1）It is dissolved in the aqueous solution, maleylation Chi（0.5 mg·mL-1）It is molten
Solution is in 0.1 M acetums, and magnetic stirrer over night makes it fully dissolve；The natural HA of comparable sodium is prepared simultaneously and natural
Chi solution is as a comparison.
Above-mentioned each solution is before use, in addition to dopamine and tHA solution, the pH value of all solution is adjusted to 4.
4. it is self-assembled modified
Polylactic acid nano spinning fibre is fully washed after being infiltrated through 75% ethanol, and 2 mgmL are transferred them to first-1DOPA
4 h are soaked in amine aqueous solution, then fully washing, make it on the dopamine basis of spinning fibre surface-assembled last layer positively charged
Layer.Nanometer spinning fibre through DOPA after amine-modified immerses in tHA and MA-Chi solution successively, after each layer of absorption 12 minutes all
Along with elution step（3×3 min）, eluent is the aqueous solution；While by nanometer spinning fibre of the DOPA after amine-modified successively
Immerse HA and in Chi solution, equal conditions processing is compared.
Repeat above step and 11 layers of HA and Chi and tHA and MA-Chi are finally repeatedly respectively obtained on dopamine basal layer
The nanometer spinning fibre of alternating sorbent.
The physical and chemical performances such as the above-mentioned shape characteristic of polylactic acid nano spinning fibre after self-assembled modified are detected,
Test result is as follows.
Physical and chemical performance is tested
（1）The identification of active group
Fig. 1 is the hyaluronic acid and the nuclear magnetic spectrogram of chitosan after before modified, it will be seen from figure 1 that after thiolated modified
The Chi molecules that are modified of HA and maleylation on be successfully incorporated into sulfydryl and double bond.In addition, by Ellman ' s test and
Maleic acid calibration curve method further confirms that sulfhydrylation and maleylation have successfully been carried out to HA and Chi to be modified, wherein mercapto
Base content is 160 μm of olg-1Left and right, acetyl group number is 1971 μm of olg-1Left and right.
（2）The appearance structure of polylactic acid nano spinning fibre after self-assembled modified
Fig. 2 is respectively pure polylactic acid nano spinning fibre（PLLA）, the amine-modified rear polylactic acid nano spinning fibre of DOPA（DA）, day
Right Chi and polylactic acid nano spinning fibre after the modification of HA LBL self-assemblies（CHI-HA）And MA-Chi and tHA LBL self-assemblies
Polylactic acid nano spinning fibre after modification（MCHI-tHA）Appearance structure.
Figure it is seen that PLA spinning fibre surface roughness has significantly after MA-Chi and tHA modifications
Improve, this shows that MA-Chi and tHA are successfully adsorbed onto on polylactic acid nano spinning fibre.
（3）Hydrophily, moisturizing, the moisture holding capacity of polylactic acid nano spinning fibre after self-assembled modified
Contact angle（WCA）Detection is the important means of reaction material surface hydrophilic and hydrophobic, with the hydrophilic increase of material surface,
Its WCA value will be reduced.
As seen from Figure 3, the hydrophily of the spinning fibre after Chi and HA or MA-Chi and tHA modifications has bright
Aobvious raising.And water suction further to material, moisture retention are found after studying, the water suction of pure PLA spinning fibre, protect
Wet ability is respectively 20.48% ± 6.44 and 12.31% ± 3.13, and after MA-Chi and tHA modifications the water suction of material, moisturizing energy
Power is up to 527.78% ± 25.46 and 56.87% ± 10.54, show the repair materials prepared by this method have good water suction,
Moistening effect.
（4）Self-assembled modified rear polylactic acid nano spinning fibre anti-microbial property
From fig. 4, it can be seen that pure polylactic acid nano spinning fibre around has no inhibition zone in itself without antibiotic property, and through natural Chi and HA
Obvious inhibition zone is also had no around polylactic acid nano spinning fibre after modification, points out it not have antibacterial ability.On the contrary, poly-
Lactic acid nanometer spinning fibre is pressed down by occurring in that obvious inhibition zone around the self-assembled modified rear spinning fibre of MA-Chi and tHA
Bacterium circle width is about 6.53mm ± 1.54, shows that the repair materials have obvious antibacterial, bacteriostasis.
（5）Self-assembled modified rear polylactic acid nano spinning fibre tissue adhension performance
Fig. 5 can try hard to for the tissue adhension of self-assembled modified front and rear polylactic acid nano spinning fibre, as shown in Figure 5, through self assembly
The tissue adhension ability of nanometer spinning fibre after especially MA-Chi and tHA is modified after modification has significant raising.We
Speculate, this is probably because the sulfydryl on tHA molecules can be formed with the subdomain rich in cysteine of glycoprotein in tissue
Two meta keys, so as to produce stronger tissue adhension ability.
Application of the repair materials that the embodiment 1 of application test example one is provided in insulin load
Found in our early-stage Studies under serum-free condition, certain density insulin has significant promotion umbilical cord mesenchyma
The ability of stem cells hyperplasia.On this basis, we are utilized with the self assembly macromolecule layer after spinning fibre modification in embodiment 1
For carrier, load of the insulin in spinning fibre is realized by way of simply immersing, and its physicochemical property is tested.
（1）Insulin slow release effect
Fig. 6 is the sustained release behavior of insulin in self-assembled modified front and rear polylactic acid nano spinning fibre, from Fig. 6 results, is passed through
Cross its sustained-release and controlled release effect of the insulin of load in the spinning fibre after MA-Chi and tHA modifications to be significantly improved, insulin
Release can continue to 15 days after.
（2）Cytology is evaluated
The key factor that biomaterial is used for skin wound reparation is to require that it has to promote wound repair ability, previous experiments
Find that certain density insulin can significantly promote umbilical cord mesenchyma to do under serum-free condition under two-dimentional condition of culture
Cell is bred.This patent finds that the spinning after being modified through MA-Chi and tHA is fine by the use of self-assembled modified layer as insulin carrier
Dimension can significantly improve the slow release effect of insulin.Fig. 7 is that different spinning fibre surfaces umbilical cord mesenchymal stem cells breed behavior,
As shown in Figure 7, the spinning fibre after load insulin can remarkably promote cell propagation, point out by using invention
The upper insulin of repair materials load can realize the effect for accelerating union of wounded skin.
The present invention has obtained a kind of with good anti-by carrying out biological functional modification to polylactic acid nano spinning fibre
Bacterium, moisturizing, the repair materials of appropriate tissue adhesiveness, while the technology can also be applied to other high molecular nanometer spinning
On fiber-modified, and other organizational project application fields.
Claims (9)
1. a kind of repair materials adhered to anti-bacteria wet keeping appropriate tissue, it is characterised in that the preparation method of the material is such as
Under：
S1：Sulfhydrylation modification is carried out to hyaluronic acid solution and controls free sulfhydryl groups content to be 100~200 μm of olg-1, through saturating
Analysis, freeze-drying produce thiolated hyaluronic acid；
S2：Maleylation modification is carried out to chitosan solution and controls acetyl content to be 1500~2500 μm of olg-1, through saturating
Analysis, freeze-drying produce Nmaleoyl chitosan；
S3. fully washed after nanometer spinning fibre is soaked through dopamine solution, nanometer spinning fibre is then immersed into mercapto successively
Adsorbed in base hyaluronic acid solution and Nmaleoyl chitosan solution, elution after having adsorbed；
S4：Repeat absorption, elution step and produce thiolated hyaluronic acid and Nmaleoyl chitosan alternating sorbent afterwards several times
Repair materials；
Wherein, the concentration of thiolated hyaluronic acid solution is 0.25~0.75 mgmL-1, the Nmaleoyl chitosan solution
Concentration be 0.25~0.75 mgmL-1。
2. there are the repair materials that anti-bacteria wet keeping appropriate tissue is adhered to according to claim 1, it is characterised in that described free
Sulfhydryl content is 120~180 μm of olg-1。
3. there are the repair materials that anti-bacteria wet keeping appropriate tissue is adhered to according to claim 1, it is characterised in that the acetyl
Base content is 1700~2000 μm of olg-1。
4. there are the repair materials that anti-bacteria wet keeping appropriate tissue is adhered to according to claim 1, it is characterised in that sulfhydrylation is saturating
The concentration of bright matter acid solution is 0.5 mgmL-1, the concentration of the Nmaleoyl chitosan solution is 0.5 mgmL-1。
5. there are the repair materials that anti-bacteria wet keeping appropriate tissue is adhered to according to claim 1, it is characterised in that repeated in S4
Absorption, elution step 9~11 times.
6. there are the repair materials that anti-bacteria wet keeping appropriate tissue is adhered to according to claim 1, it is characterised in that sulfydryl in S3
The pH for changing hyaluronic acid solution is 5~7, and the pH of Nmaleoyl chitosan solution is 3~5.
What 7. claim 1~6 was provided has the repair materials that anti-bacteria wet keeping appropriate tissue is adhered to as carrier in water-soluble pesticide
Application in thing and bioactie agent load.
What 8. claim 1~6 was provided has the repair materials of anti-bacteria wet keeping appropriate tissue adhesion in field of tissue engineering technology
Using.
9. application according to claim 8, it is characterised in that application of the repair materials in skin repair field.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113616675A (en) * | 2021-08-23 | 2021-11-09 | 上海太安堂生物医学有限公司 | Composition containing mesenchymal stem cells and application thereof in treating degenerative arthritis |
| CN113818244A (en) * | 2021-08-03 | 2021-12-21 | 广东医科大学附属医院 | Intramolecular cross-linking self-assembled membrane modified spinning nanofiber material and preparation method and application thereof |
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| CN115058892A (en) * | 2022-06-17 | 2022-09-16 | 温州医科大学 | Lactic acid porous fiber membrane and application thereof |
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