CN103091253B - A kind ofly eliminate the interference method of side-lobe signal, system and blood cell analyzer - Google Patents
A kind ofly eliminate the interference method of side-lobe signal, system and blood cell analyzer Download PDFInfo
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Abstract
The present invention is applicable to medical signals processing technology field, provide and a kind ofly eliminate the interference method of side-lobe signal, system and blood cell analyzer, described method comprises the steps: to receive cell through swashing light-struck scattered light signal, convert described scattered light signal to digital signal, and obtain the signal characteristic information in described digital signal; According to described signal characteristic information, search the interference side-lobe signal in described digital signal; Reject the interference side-lobe signal in described digital signal.The present invention realizes when using optical scattering method identification cell, such as platelet cell, do not need just can eliminate interference side-lobe signal by improving optical component parameter, reduce optical component parameter requirement, thus reduce Material Cost, and improve the accuracy using optical scattering method identification cell.
Description
Technical field
The invention belongs to medical signals processing technology field, particularly relate to and a kind ofly eliminate the interference method of side-lobe signal, system and blood cell analyzer.
Background technology
The method that blood cell analyzer carries out haemocyte detection mainly contains two kinds: one is impedance method, and another kind is optical method.Early stage blood analyser majority all adopts impedance method to measure haemocyte, and the concrete principle of impedance method is Coulter principle, and when utilizing haemocyte to flow through aperture, the change of aperture two terminal impedance detects.Along with the progress of Cell Measurement Technique, optical method arises at the historic moment, its concrete principle is irradiated to by the laser of specific wavelength to flow through on the haemocyte of irradiated region, by collecting scattered light or the combined with fluorescent of the multiple angle of haemocyte, realizes the detection to haemocyte with multidimensional information.Relative to traditional impedance method, optical method sensitivity when detecting haemocyte is higher, and affect less by blood sample, accuracy is also higher.Therefore, high-end blood analyser generally all possesses the ability that optical method detects haemocyte.
Adopting the blood cell analyzer of optical method when measuring haemocyte, distinguishing the volume size of cell mainly through low-angle scattered light, and in conjunction with the scattered light of other angles or fluorescence, cell is distinguished.When detecting the cell of some small sizes, as platelet cell, due to its volume ratio red blood cell and leucocyte much smaller, therefore, the main lobe peak value of the low angle scattered light produced when such stream of cells crosses laser irradiation area is also less.Due to optical element inherent parameters, as aperture restriction and the ripple difference of lens, the main lobe of scattered light is neighbouring generally all can exist secondary lobe, the size of side lobe peak and the size of main lobe peak value and optical element inherent parameters closely related.Under the condition that optical component parameter index is not high, the cell that volume is larger, such as, red blood cell and leucocyte, side lobe peak near the low-angle scattered light main lobe produced and the cell of small volume, such as, platelet cell, the low angle scattered light main lobe peak value produced is comparatively close, and difference is little.When measuring, above-mentioned secondary lobe can disturb the detection accuracy of small size cell.As when measuring blood platelet, above-mentioned secondary lobe can be divided into platelet cell, causes the hematoblastic measured value of optical method higher.
Prior art by improving the parameter request of optical element, the scattered light side lobe peak produced to make large volume cell much smaller than the scattered light main lobe peak value of small size cell, as expanded the aperture of lens, the ripple difference etc. of reduction lens.But conventionally provided technical scheme, find to there is following technical matters in prior art: prior art eliminates interference side-lobe signal by improving optical component parameter performance requirement, due to higher to the parameter request of optical element, cost is caused significantly to rise.
Summary of the invention
The object of the embodiment of the present invention is to provide a kind of method eliminating interference side-lobe signal, be intended to solve prior art and eliminate interference side-lobe signal by improving optical component parameter performance requirement, due to higher to the parameter request of optical element, cause the problem that cost significantly rises.
The embodiment of the present invention is achieved in that a kind of method eliminating interference side-lobe signal, and described method comprises the steps:
Receiving cell through swashing light-struck scattered light signal, converting described scattered light signal to digital signal, and obtaining the signal characteristic information in described digital signal;
According to described signal characteristic information, search the interference side-lobe signal in described digital signal;
Reject the interference side-lobe signal in described digital signal.
The embodiment of the present invention additionally provides a kind of system eliminating interference side-lobe signal, and described system comprises:
Information acquisition unit, for receiving cell through swashing light-struck scattered light signal, converting described scattered light signal to digital signal, and obtaining the signal characteristic information in described digital signal;
Secondary lobe searches unit, for according to described signal characteristic information, searches the interference side-lobe signal in described digital signal;
Culling unit, for rejecting the interference side-lobe signal in described digital signal.
The embodiment of the present invention additionally provides a kind of blood cell analyzer, and described blood cell analyzer comprises the system of above-mentioned elimination interference side-lobe signal.
In the present embodiment, by receiving cell through swashing light-struck scattered light signal, convert described scattered light signal to digital signal, and the signal characteristic information obtained in described digital signal, according to described signal characteristic information, search the interference side-lobe signal in described digital signal, reject the interference side-lobe signal in described digital signal.Realize when using optical scattering method identification cell, such as platelet cell, not needing just can eliminate interference side-lobe signal by improving optical component parameter, reducing optical component parameter requirement, thus reduce Material Cost, and improve the accuracy using optical scattering method identification cell.
Accompanying drawing explanation
Fig. 1 is the process flow diagram of the realization of the method for the elimination interference side-lobe signal that the embodiment of the present invention one provides;
Fig. 2 is the process flow diagram of the realization of the method for the elimination interference side-lobe signal that the embodiment of the present invention two provides;
Fig. 3 is the digital signaling diagram that the embodiment of the present invention two provides;
Fig. 4 is the design sketch not using the method thromboblast eliminating interference side-lobe signal to measure that the embodiment of the present invention two provides;
Fig. 5 be the embodiment of the present invention two provide employ eliminate interference side-lobe signal method after blood platelet measure design sketch;
Fig. 6 is the system construction drawing of the application scenarios that the embodiment of the present invention three provides;
Fig. 7 is the structural drawing of the system of the elimination interference side-lobe signal that the embodiment of the present invention three provides;
Fig. 8 is the structural drawing of the system of the elimination interference side-lobe signal that the embodiment of the present invention four provides;
Fig. 9 is the structural drawing of the system of the elimination interference side-lobe signal that the embodiment of the present invention five provides.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
The embodiment of the present invention, by processing through swashing light-struck scattered light signal cell, according to signal characteristic information, searching the interference secondary lobe in digital signal, being removed by the secondary lobe near main lobe signal.
Embodiments provide a kind of method eliminating interference side-lobe signal, described method comprises the steps:
Receiving cell through swashing light-struck scattered light signal, converting described scattered light signal to digital signal, and obtaining the signal characteristic information in described digital signal;
According to described signal characteristic information, search the interference side-lobe signal in described digital signal;
Reject the interference side-lobe signal in described digital signal.
The embodiment of the present invention additionally provides a kind of system eliminating interference side-lobe signal, and described system comprises:
Information acquisition unit, for receiving cell through swashing light-struck scattered light signal, converting described scattered light signal to digital signal, and obtaining the signal characteristic information in described digital signal;
Secondary lobe searches unit, for according to described signal characteristic information, searches the interference side-lobe signal in described digital signal;
Culling unit, for rejecting the interference side-lobe signal in described digital signal.
The embodiment of the present invention additionally provides a kind of blood cell analyzer, and described blood cell analyzer comprises the system of above-mentioned elimination interference side-lobe signal.
Below in conjunction with specific embodiment, realization of the present invention is described in detail:
embodiment one
The application scenarios of the present embodiment can be: adopt the blood cell analyzer of optical method when identifying measurement cell, such as platelet cell, the scattered light signal that cell produces after laser irradiates carries out opto-electronic conversion by sensor, electric analoging signal is changed into by light signal, electric analoging signal after conversion is carried out signal shaping, such as, amplify, filtering or amplitude limit etc., all be defined within the specific limits to make the frequency of electric signal and amplitude, electric analoging signal after signal shaping is carried out digital quantization, obtain corresponding digital signal, by processing this digital signal, to remove the impact of secondary lobe on measured cell, such as, red blood cell or leukocytic side-lobe signal are the interference secondary lobe of platelet cell, be specifically described by the following examples, Fig. 1 shows the process flow diagram of the realization of the method for the elimination interference side-lobe signal that the embodiment of the present invention one provides, details are as follows:
In step S101, receiving cell through swashing light-struck scattered light signal, converting described scattered light signal to digital signal, and obtaining the signal characteristic information in described digital signal.
In the present embodiment, described signal characteristic information is the combination in any of peak value of pulse, peak value of pulse time or pulse base, but is not limited to above three kinds of signal characteristic informations.
In the present embodiment, described digital signal is the signal of scattered light signal after opto-electronic conversion, signal shaping and digital quantization.
In step s 102, according to described signal characteristic information, search the interference side-lobe signal in described digital signal.
In the present embodiment, in the process of undesired signal of searching digital signal, need the signal characteristic information using multiple pulse signal, therefore, usual pulse signal in chronological sequence arrives respectively, and during the pulse signal feature arrived before needing backtracking, the feature of these pulse signals is not if carried out buffer memory, pulse signal then may be caused to lose, therefore, in order to solve the problem, optionally, can also comprise the steps before step S102, the signal characteristic information that buffer accepts arrives.
In the present embodiment, the process that step S102 searches can in the following ways:
Mode one: the time order and function order arrived according to pulse, and according to described signal characteristic information, successively interference secondary lobe is searched to each pulse signal, be specially: after having searched the secondary lobe near current PRF, judge whether current PRF is last pulse, if not last pulse, then near next pulse, searches secondary lobe, when last pulse of arrival, then stop searching.
Mode two: in order to improve seek rate, according to signal characteristic information, interference secondary lobe can be searched to multiple pulse signal simultaneously, such as, can search two pulses simultaneously, three pulses, can certainly search interference secondary lobe to all pulse signals of current arrival simultaneously.
In step s 103, the interference side-lobe signal in described digital signal is rejected.
Optionally, often can find an interference secondary lobe, then in described digital signal, reject this interference side-lobe signal.
Optionally, also can after having searched all interference side-lobe signals of digital signal, unified all interference side-lobe signals of rejecting in digital signal, now, delete interference side-lobe signal for the ease of unified, step S102 can also comprise the steps: identifier lookup to described digital signal in interference secondary lobe, or store the signal characteristic information of the interference secondary lobe in the described digital signal found, such as, the peak value of pulse information of interference secondary lobe, or peak value of pulse temporal information etc.
The present embodiment method can realize the identification to haemocyte, and especially to the identification of small size cell, such as, molecule etc. in platelet cell, blood, therefore optionally, after step S103, can also comprise the steps:
According to the digital signal rejected after interference side-lobe signal, identify haemocyte.
In the present embodiment, by receiving cell through swashing light-struck scattered light signal, convert described scattered light signal to digital signal, and the signal characteristic information obtained in described digital signal, according to described signal characteristic information, search the interference side-lobe signal in described digital signal, reject the interference side-lobe signal in described digital signal.Realize when using optical scattering method identification cell, such as platelet cell, not needing just can eliminate interference side-lobe signal by improving optical component parameter, reducing optical component parameter requirement, thus reduce Material Cost, and improve the accuracy using optical scattering method identification cell.
embodiment two
Fig. 2 shows the process flow diagram of the realization of the method for the elimination interference side-lobe signal that the embodiment of the present invention two provides, and details are as follows:
In step s 201, receive cell through swashing light-struck scattered light signal, convert described scattered light signal to digital signal, and obtain the signal characteristic information in described digital signal, described signal characteristic information is peak value of pulse, peak value of pulse time and pulse base.
In step S202, remove the pulse base comprised in described peak value of pulse, obtain pulse relative peak.
In the present embodiment, step S202 is specifically as follows and deducts described pulse base from described peak value of pulse, obtain pulse relative peak, wherein, described pulse base can be deducted by signal processing mode from described peak value of pulse, or also can remove by hardware circuit the pulse base comprised in peak value of pulse, such as, can subtraction circuit be passed through, certainly, be not limited to above-mentioned implementation.
In step S203, according to described pulse relative peak, determine secondary lobe discrimination threshold.
In the embodiment of the present invention, step S203 specifically can realize in the following ways:
Mode one: long-pending according to each pulse relative peak and secondary lobe scale-up factor, determine the secondary lobe discrimination threshold of each pulse, be specially and each pulse relative peak is multiplied with secondary lobe scale-up factor as the secondary lobe discrimination threshold of each pulse, such as, if secondary lobe scale-up factor is 15%, after then each peak value of pulse being all multiplied by 15%, as secondary lobe discrimination threshold, wherein, secondary lobe scale-up factor can be obtained by the ratio of secondary lobe and main lobe in the optical signalling of statistics reality, is specifically as follows side-lobe pulse relative peak divided by main lobe pulse relative peak.
Mode two: in order to overcome the problem that in mode one, calculated amount is larger, can according to the statistical value of pulse relative peak or measured value, determine that preset value is the secondary lobe discrimination threshold of all pulses, that is, all pulses all use a fixing threshold value to carry out secondary lobe as secondary lobe discrimination threshold to search.
In step S204, according to described secondary lobe discrimination threshold, in the Preset Time before and after described peak value of pulse, search the interference side-lobe signal in described digital signal.
In the present embodiment, can in current PRF pre-and post-peaking Preset Time, such as: Preset Time can be 3us, searches the pulse that pulse relative peak is less than pulse secondary lobe discrimination threshold.
In step S205, reject the signal characteristic information of the described interference side-lobe signal in described digital signal.
In the present embodiment, the signal characteristic information of the interference secondary lobe of rejecting specifically comprises: the peak value of pulse of interference side-lobe signal and peak value of pulse time data.
In the present embodiment, the implementation of the step S103 in the execution of step S205 and above-described embodiment one is similar, and details are see the description of above-described embodiment one.
For the ease of understanding, be described with the concrete technical scheme of realization example to the present embodiment below, but be not limited with the situation of the present embodiment, Fig. 3 is a digital signaling diagram, wherein, horizontal ordinate is time value, ordinate is pulse amplitude values, with this figure, the concrete process of searching secondary lobe is described below: first, feel the pulse from 1 and wash the beginning open and carry out secondary lobe and search, calculate 1 feel the pulse punching relative peak, feel the pulse 1 punching relative peak be multiplied by secondary lobe ratio K obtain 1 feel the pulse punching secondary lobe discrimination threshold (THR1), 1 feel the pulse punching secondary lobe critical region in find the pulse that pulse relative peak is less than THR1, wherein secondary lobe critical region is 1 feel the pulse and rush pre-and post-peaking Preset Time.Whole 1 feel the pulse punching secondary lobe discriminate interval in only have a pulse to meet criterion, namely 2 to feel the pulse punching, therefore 2 punchings of feeling the pulse can be marked as interference secondary lobe.Although 2 punchings of feeling the pulse are marked as secondary lobe, terminating to feel the pulse after the searching of secondary lobe near punching to 1, the pulse that the next one searches secondary lobe remains 2 and feels the pulse and rush instead of 3 to feel the pulse punching.Feel the pulse rush the same in 2 the feel the pulse methods and 1 of searching secondary lobe near punching, feel the pulse after the searching of the side-lobe signal that rushes annex to 2 completing, start to search 3 and to feel the pulse the side-lobe signal near rushing.Whole process of searching side-lobe signal just so successively one by one pulse go on, until complete searching all pulse side-lobe signals.
Fig. 4 and Fig. 5 is that wherein, horizontal ordinate FL is fluorescence, and ordinate FSC is low angle scattered light to the effect contrast figure of identical data before and after the operation of application the present embodiment elimination interference side-lobe signal.Fig. 4 is the design sketch that the method thromboblast not using the present embodiment to eliminate interference side-lobe signal is measured, can see in the diagram, contain because the blood platelet that identifies of interference side-lobe signal by mistake in the region being designated A, obviously can see that from the A of region abnormal noise spot has appearred in the left side in normal blood platelet region, and mix with blood platelet, be difficult to distinguish; Fig. 5 is the design sketch that after identical data employs the method for the present embodiment elimination interference side-lobe signal, blood platelet is measured, and can see in Figure 5, not disturb the noise spot that secondary lobe brings in the B of region.
The effect of the method for interference side-lobe signal is eliminated in order to further illustrate the present embodiment, be described with concrete measurement data below, refer to table 1, table 1 adopts impedance method and optical method respectively to same blood sample and adopts the blood platelet measurement result that in Fig. 4 and Fig. 5, measurement data calculates:
Table 1
As can be seen from Table 1, when the data before adopting Fig. 4 to reject interference secondary lobe calculate blood platelet measured value, cause result more higher than actual value owing to part noise spot can be counted among blood platelet in the lump.Further, under normal circumstances, because optical method is to the discrimination specific impedance Fa Genggao of platelet cell, can by impedance method identify less than blood platelet to identifying, therefore the result that general identical blood sample uses optical method to record wants the slightly higher of specific impedance method.Table 1 shows that the blood platelet value that the same blood sample used is tested under impedance method is 204 × 10
9/ L, according to the feature of optical method, using optical method to test the result obtained should than 204 × 10
9/ L is more higher.By contrasting the result of upper table, can clearly find out: before rejecting interference secondary lobe, the hematoblastic measured value of optical method reaches more than 2 times of the hematoblastic measured value of impedance method, serious distortion; After rejecting interference secondary lobe, optical method blood platelet measured value returns to normal level, comparatively close with impedance method blood platelet measured value.
The present embodiment passes through according to described peak value of pulse and pulse base, calculate pulse relative peak, according to described pulse relative peak, determine secondary lobe discrimination threshold, and according to described secondary lobe discrimination threshold, in Preset Time before and after described peak value of pulse, search the interference side-lobe signal in described digital signal, and reject peak value of pulse and the peak value of pulse time data of the interference side-lobe signal in described digital signal, realize when using optical scattering method identification haemocyte, do not need just can eliminate interference side-lobe signal by improving optical component parameter, reduce optical component parameter requirement, thus Material Cost is reduced, and improve the accuracy using optical scattering method identification haemocyte.
embodiment three
Fig. 6 is the system construction drawing of the application scenarios of the present embodiment: adopting the blood cell analyzer of optical method when measuring cell, such as platelet cell, the scattered light signal that cell produces after laser irradiates carries out opto-electronic conversion by sensor 61, electric signal is changed into by light signal, electric analoging signal after conversion is carried out signal shaping through signal conditioning circuit 62, such as, amplify, filtering or amplitude limit etc., all be defined within the specific limits to make the frequency of electric signal and amplitude, electric analoging signal after signal shaping is carried out digital quantization through analog to digital conversion circuit 63, obtain corresponding digital signal, digital signal enters the system 64 eliminating interference side-lobe signal, this system processes this digital signal, to remove the one-level secondary lobe or multistage secondary lobe that affect measured cell, such as, red blood cell or leukocytic side-lobe signal are the interference secondary lobe of platelet cell, below be specifically specifically described by embodiment, Fig. 7 shows the structural drawing of the system of the elimination interference side-lobe signal that the embodiment of the present invention three provides, for convenience of explanation, illustrate only the part relevant to the embodiment of the present invention, this device can be the software unit be built in blood cell analyzer, hardware cell or soft or hard combining unit.
The system that the present embodiment eliminates interference side-lobe signal specifically can comprise: information acquisition unit 71, secondary lobe search unit 72, culling unit 73.
Information acquisition unit 71, for receiving cell through swashing light-struck scattered light signal, converting described scattered light signal to digital signal, and obtaining the signal characteristic information in described digital signal.
Secondary lobe searches unit 72, for according to described signal characteristic information, searches the interference side-lobe signal in described digital signal.
Culling unit 73, for rejecting the interference side-lobe signal in described digital signal.
The system of the elimination interference side-lobe signal that the embodiment of the present invention provides can be used in the embodiment of the method one of aforementioned correspondence, and details, see the description of above-described embodiment one, do not repeat them here.
In the present embodiment, by receiving cell through swashing light-struck scattered light signal, convert described scattered light signal to digital signal, and the signal characteristic information obtained in described digital signal, according to described signal characteristic information, search the interference side-lobe signal in described digital signal, reject the interference side-lobe signal in described digital signal.Realize when using optical scattering method identification cell, such as platelet cell, not needing just can eliminate interference side-lobe signal by improving optical component parameter, reducing optical component parameter requirement, thus reduce Material Cost, and improve the accuracy using optical scattering method identification cell.
embodiment four
Fig. 8 shows the structural drawing of the system of the elimination interference side-lobe signal that the embodiment of the present invention four provides, for convenience of explanation, illustrate only the part relevant to the embodiment of the present invention, this device can be the software unit be built in blood cell analyzer, hardware cell or soft or hard combining unit.
The system that the present embodiment eliminates interference side-lobe signal specifically can comprise: information acquisition unit 81, buffer unit 82, secondary lobe search unit 83, culling unit 84 and recognition unit 85.
Information acquisition unit 81, for receiving cell through swashing light-struck scattered light signal, converting described scattered light signal to digital signal, and obtaining the signal characteristic information in described digital signal;
Optionally, buffer unit 82, for signal characteristic information described in buffer memory.
Secondary lobe searches unit 83, for according to described signal characteristic information, searches the interference side-lobe signal in described digital signal.
Culling unit 84, for rejecting the interference side-lobe signal in described digital signal.
Recognition unit 85, for according to the digital signal rejected after interference side-lobe signal, identifies haemocyte.
Optionally, secondary lobe searches unit 83, specifically for the time order and function order according to pulse, and according to described signal characteristic information, searches interference secondary lobe successively to each pulse signal.Or secondary lobe searches unit 83, specifically for according to described signal characteristic information, interference secondary lobe is searched to multiple pulse signal simultaneously.
The system of the elimination interference side-lobe signal that the embodiment of the present invention provides can be used in the embodiment of the method one of aforementioned correspondence, and details, see the description of above-described embodiment one, do not repeat them here.
embodiment five
Fig. 9 shows the structural drawing of the system of the elimination interference side-lobe signal that the embodiment of the present invention five provides, for convenience of explanation, illustrate only the part relevant to the embodiment of the present invention, this device can be the software unit be built in blood cell analyzer, hardware cell or soft or hard combining unit.
The system that the present embodiment eliminates interference side-lobe signal specifically can comprise: information acquisition unit 91, buffer unit 92, secondary lobe search unit 93, culling unit 94 and recognition unit 95.
Described secondary lobe is searched unit 93 and is specifically comprised: baseline is removed module 931, determination module 932 and searched module 933.
Baseline removes module 931, for removing the pulse base comprised in described peak value of pulse, obtains pulse relative peak.
In the present embodiment, baseline removal module 931 is specifically as follows and deducts described pulse base from described peak value of pulse, obtain pulse relative peak, wherein, described pulse base can be deducted by signal processing mode from described peak value of pulse, or also can remove by hardware circuit the pulse base comprised in peak value of pulse, such as, can subtraction circuit be passed through, certainly, be not limited to above-mentioned implementation.
Determination module 932, for according to described pulse relative peak, determines secondary lobe discrimination threshold.
Search module 933, for according to described secondary lobe discrimination threshold, in the Preset Time before and after described peak value of pulse, search the interference side-lobe signal in described digital signal.
Described culling unit 94, specifically for rejecting the signal characteristic information of the described interference side-lobe signal in described digital signal
Wherein, determination module 932, long-pending specifically for according to each pulse relative peak and secondary lobe scale-up factor, determines the secondary lobe discrimination threshold of each pulse;
Or described determination module 932, specifically for according to the statistical value of pulse relative peak or measured value, determines that preset value is the secondary lobe discrimination threshold of all pulses.
The system of the elimination interference side-lobe signal that the embodiment of the present invention provides can be used in the embodiment of the method two of aforementioned correspondence, and details, see the description of above-described embodiment two, do not repeat them here.
It should be noted that in said apparatus and system embodiment, included unit is carry out dividing according to function logic, but is not limited to above-mentioned division, as long as can realize corresponding function; In addition, the concrete title of each functional unit, also just for the ease of mutual differentiation, is not limited to protection scope of the present invention.
In addition, one of ordinary skill in the art will appreciate that all or part of step realized in the various embodiments described above method is that the hardware that can carry out instruction relevant by program has come, corresponding program can be stored in a computer read/write memory medium, described storage medium, as ROM/RAM, disk or CD etc.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (15)
1. eliminate a method for interference side-lobe signal, it is characterized in that, described method comprises the steps:
Receiving cell through swashing light-struck scattered light signal, converting described scattered light signal to digital signal, and obtaining the signal characteristic information in described digital signal;
According to described signal characteristic information, search the interference side-lobe signal in described digital signal;
Reject the interference side-lobe signal in described digital signal;
Wherein, described signal characteristic information is peak value of pulse, peak value of pulse time and pulse base; Described according to described signal characteristic information, the step of searching the interference side-lobe signal in described digital signal is specially:
Remove the pulse base comprised in described peak value of pulse, obtain pulse relative peak;
According to described pulse relative peak, determine secondary lobe discrimination threshold;
According to described secondary lobe discrimination threshold, in the Preset Time before and after described peak value of pulse, search the interference side-lobe signal in described digital signal.
2. the method for claim 1, is characterized in that, after the step of the interference side-lobe signal in the described digital signal of described rejecting, described method also comprises the steps:
According to the digital signal rejected after interference side-lobe signal, identify haemocyte.
3. the method for claim 1, is characterized in that, described according to described signal characteristic information, and before searching the step of the interference side-lobe signal in described digital signal, described method also comprises the steps:
Signal characteristic information described in buffer memory.
4. the method for claim 1, is characterized in that, the step of the interference side-lobe signal in the described digital signal of described rejecting is specially:
Reject the signal characteristic information of the described interference side-lobe signal in described digital signal.
5. method as claimed in claim 4, is characterized in that, described according to described pulse relative peak, determines that the step of secondary lobe discrimination threshold is specially:
Long-pending according to each pulse relative peak and secondary lobe scale-up factor, determines the secondary lobe discrimination threshold of each pulse; Or
According to statistical value or the measured value of pulse relative peak, determine that preset value is the secondary lobe discrimination threshold of all pulses.
6. method as claimed in claim 4, it is characterized in that, the pulse base comprised in described removal peak value of pulse, the step obtaining pulse relative peak is specially:
From described peak value of pulse, deduct described pulse base, obtain pulse relative peak.
7. the method as described in claim as arbitrary in claim 1 to 6, is characterized in that, according to described signal characteristic information, the step of searching the interference side-lobe signal in described digital signal is specially:
According to the time order and function order of pulse, and according to described signal characteristic information, successively interference secondary lobe is searched to each pulse signal; Or
According to described signal characteristic information, interference secondary lobe is searched to multiple pulse signal simultaneously.
8. eliminate a system for interference side-lobe signal, it is characterized in that, described system comprises:
Information acquisition unit, for receiving cell through swashing light-struck scattered light signal, converting described scattered light signal to digital signal, and obtaining the signal characteristic information in described digital signal;
Secondary lobe searches unit, for according to described signal characteristic information, searches the interference side-lobe signal in described digital signal;
Culling unit, for rejecting the interference side-lobe signal in described digital signal;
Described signal characteristic information is peak value of pulse, peak value of pulse time and pulse base;
Described secondary lobe is searched unit and is specifically comprised:
Baseline removes module, for removing the pulse base comprised in described peak value of pulse, obtains pulse relative peak;
Determination module, for according to described pulse relative peak, determines secondary lobe discrimination threshold;
Search module, for according to described secondary lobe discrimination threshold, in the Preset Time before and after described peak value of pulse, search the interference side-lobe signal in described digital signal.
9. system as claimed in claim 8, it is characterized in that, described system also comprises:
Recognition unit, for according to the digital signal rejected after interference side-lobe signal, identifies haemocyte.
10. system as claimed in claim 8, it is characterized in that, described system also comprises:
Buffer unit, for signal characteristic information described in buffer memory.
11. systems as claimed in claim 8, is characterized in that, described culling unit is specifically for rejecting the signal characteristic information of the described interference side-lobe signal in described digital signal.
12. systems as claimed in claim 11, is characterized in that, described determination module, long-pending specifically for according to each pulse relative peak and secondary lobe scale-up factor, determine the secondary lobe discrimination threshold of each pulse;
Or described determination module, specifically for according to the statistical value of pulse relative peak or measured value, determines that preset value is the secondary lobe discrimination threshold of all pulses.
13. systems as claimed in claim 11, is characterized in that, described baseline remove module specifically for:
From described peak value of pulse, deduct described pulse base, obtain pulse relative peak.
14. systems as described in any one of claim 8 to 13, it is characterized in that, secondary lobe searches unit, specifically for the time order and function order according to pulse, and according to described signal characteristic information, searches interference secondary lobe successively to each pulse signal; Or
According to described signal characteristic information, interference secondary lobe is searched to multiple pulse signal simultaneously.
15. 1 kinds of blood cell analyzers, is characterized in that, described blood cell analyzer comprises the system of the elimination interference side-lobe signal described in the arbitrary claim of claim 8 to 14.
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| CN109283121B (en) * | 2018-10-11 | 2021-04-09 | 迈克医疗电子有限公司 | Pulse recognition method and device, analysis instrument and storage medium |
| CN114674729B (en) * | 2022-03-02 | 2023-11-21 | 迈克医疗电子有限公司 | Pulse identification method, pulse identification device, pulse identification storage medium, pulse identification equipment and blood cell analyzer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| NL7314320A (en) * | 1972-12-15 | 1974-06-18 | ||
| CN101000306A (en) * | 2006-01-09 | 2007-07-18 | 深圳迈瑞生物医疗电子股份有限公司 | Cell analyser |
| CN101153868A (en) * | 2006-09-30 | 2008-04-02 | 深圳迈瑞生物医疗电子股份有限公司 | Stream type cell analyzer |
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| JPH0251044A (en) * | 1988-08-12 | 1990-02-21 | Omron Tateisi Electron Co | Cell analyzer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| NL7314320A (en) * | 1972-12-15 | 1974-06-18 | ||
| CN101000306A (en) * | 2006-01-09 | 2007-07-18 | 深圳迈瑞生物医疗电子股份有限公司 | Cell analyser |
| CN101153868A (en) * | 2006-09-30 | 2008-04-02 | 深圳迈瑞生物医疗电子股份有限公司 | Stream type cell analyzer |
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Effective date of registration: 20180806 Address after: 518057 the 1-4 floor of MINDRAY building, science and technology south twelve Road, Nanshan District high tech Industrial Park, Shenzhen, Guangdong. Co-patentee after: SHENZHEN MINDRAY SCIENTIFIC Co.,Ltd. Patentee after: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS Co.,Ltd. Address before: 518057 MINDRAY science and technology south twelve road MINDRAY high tech Industrial Park, Shenzhen, Guangdong Patentee before: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS Co.,Ltd. |
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Application publication date: 20130508 Assignee: Shenzhen Mindray Animal Medical Technology Co.,Ltd. Assignor: SHENZHEN MINDRAY BIO-MEDICAL ELECTRONICS Co.,Ltd. Contract record no.: X2022440020009 Denomination of invention: A method, system and blood cell analyzer for eliminating interference sidelobe signals Granted publication date: 20160302 License type: Common License Record date: 20220804 |