CN103091147A - Automated multilevel biological sample filtering system - Google Patents
Automated multilevel biological sample filtering system Download PDFInfo
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- CN103091147A CN103091147A CN2011103417713A CN201110341771A CN103091147A CN 103091147 A CN103091147 A CN 103091147A CN 2011103417713 A CN2011103417713 A CN 2011103417713A CN 201110341771 A CN201110341771 A CN 201110341771A CN 103091147 A CN103091147 A CN 103091147A
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Abstract
The invention relates to an automated multilevel biological sample filtering system comprising a vacuuming device, a multilevel biological sample filter, a flow rate monitoring device, a gas-water separator, a sample water container, a system controller, and a call transfer device. The system provided by the invention is suitable for wild water sample collection and processing of various biological science experiments. In application, with the system, collection of samples of various particle size levels can be realized in one time; operation processes are effectively reduced; wild work land occupation is greatly reduced; the possibility of original sample pollution is reduced; and working speed is increased on a basis of ensured accuracy. According to the invention, full-process monitoring is carried out over collection work; unmanned operation is realized, experiment work accuracy is increased; wild operator number can be reduced; and scientific research personnel labor intensity in arduous expedition work can be greatly reduced. According to the device provided by the invention, combination forms can be easily changed. The system provided by the invention has good compatibility with traditional experimental operations.
Description
Technical field
The present invention's invention relates to ecological microbial technique and oceanographic survey technology.A kind of disposable automatic intelligent collection by the multi-stage vacuum layering of water sample that biology can be gathered is filtered system specifically.
Background technology
In the sea life science and technology field, a large amount of seawater samples is carried out suction filtration, obtain the required raw data of different majors, be a kind of basic skills of biological scientific experiment and sample collection.
Up to now, owing to lacking targetedly, being fit to the marine collection filter for biological sample equipment required with field work, relevant experimental work also needs to obtain one by one through a large amount of, that repeat, gradual method at present.For example, be engaged in so far this experimental work and rely on simple pumping and filtering device fully, the different grain size level sample that experiment is required, need respectively to carry out for several times to filter journey by thick gradually thin collection, not only labour intensity is very big, also need long-time special messenger to look after, to the also increase unavoidably in this process of pollution of water sample.The use of this kind equipment also has the larger problem that takes up space, and has strengthened working pressure marine, open-air limited lab space.
The automatic multi-layer level collection filter for biological sample that at present, can be provided as biological scientific experiment and oceanographic survey work has no any report.
Summary of the invention
For above shortcomings part in prior art, the technical problem to be solved in the present invention is to utilize the vacuum filtration method, by airtight tubular structure, and the disposable a plurality of test specimens that obtain different grain size level demand; And can in apparatus of the present invention normal work period, realize unmanned fully.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
A kind of automatic multi-layer level of the present invention biological sample collection filter system, the inlet and outlet pipe that the Multi-level collection filter for biological sample two ends pick out is connected with flow monitoring device with the sample water container respectively, flow monitoring device is connected with gas and water separator by pipeline, and gas and water separator is connected by pipeline with vaccum-pumping equipment; The draining control valve of flow detector, moisture trap, Call Transfer device and vaccum-pumping equipment are connected with system controller respectively;
Described Multi-level collection filter for biological sample be by a cover collection filter cylinder and filter membrane clamping kit consist of according to primary filter, middle rank filters, the collection filter device that the order of final stage filtration (fine filtering) assembles, its two ends are upper and lower linkage section, draw respectively inlet and outlet pipe; The bottom of collection filter cylinder is provided with a filter membrane clamping kit groove, inserts the filter membrane clamping kit in filter membrane clamping kit groove.
Described collection filter cylinder uses the cylinder material of seawater corrosion resistance to make, there are respectively outer tooth interface and Nei Ya interface in two ends, the seal groove that annular also is set respectively at two ends, barrel up and down can be placed " O " RunddichtringO, and the collection filter cylinder adopts screw thread to spin fastening by outer tooth interface and Nei Ya interface.
The mode that described collection filter cylinder can also adopt hinge to compress connects.
Described filter membrane clamping kit is comprised of upper clamping kit, lower clamping kit and filter membrane; Upper clamping kit adopts the hollow resistant material, and upper surface is smooth can combine closely with the filter membrane clamping kit groove of collection filter cylinder, and lower surface is provided with groove and the engagement of lower clamping kit upper surface tongue; Lower clamping kit is the flat board of a densely covered micropore, and lower surface is smooth flat; Filter membrane device is in the center of lower clamping kit.
Described filter membrane clamping kit can be changed filter membrane and the up and down clamping kit of different size according to different purposes.
Described Multi-level collection filter for biological sample can be selected single group direct mode and more to make up the connected mode of dividing into groups and be connected with the sample water vessel with flow monitoring device.
The air intake opening of described sample water container is provided with air strainer.
Also comprise the water level monitoring induction installation that is connected on system controller in described moisture trap.
Described system controller can be sampled in the scope of 0.5L～20L, and its sampling operation step is:
The assembling acquisition system arranges the water sampling amount;
Start acquisition system, reach setting value when flow monitoring device detects water sample, system controller cuts off the power supply of vacuum extractor, and the signal of ringing simultaneously quits work;
In the signal ended of ringing 30 seconds, the prosthetic operation gets involved, and sends wireless paging signals.
Described system controller inside is provided with dry cell, and during the external power source outage, system controller sends by dry cell power supply the signal of ringing, and sends prompting to the staff.
The present invention has the following advantages:
1. use automatic multi-layer level biological sample collection filter equipment greatly to reduce the pollution possibility of sample water sample.Because the most of work of the present invention is in vacuum state, unique replenish air mouth aperture is very little, and the filtration unit of setting has completely cut off air pollution source, and water sample is in the virgin state of collection all the time, and the confidence level of sample preparation is increased greatly.
2. the data collection task that is applicable to biology, microorganism that the present invention can be wide in range.Every have the work that collection filter requires (no matter seawater or fresh water, apparatus of the present invention are not limited to the medium of water) to water sample, and the present invention all can satisfy the demand.
3. the present invention is owing to no longer needing to carry out packing to gathering water sample, also do not need the suction filtration of repeatedly being correlated with for obtaining the different grain size level, add the control system and the paging system that have possessed the water sample total amount, make on the spot that the labour intensity of sampling work greatly reduces, also effectively controlled the experiment error rate.
4. the present invention uses the less corrosion-resistant combination of sets filter cylinder of diameter, the total length of each joint collection filter cylinder addition is no more than 50cm, not only effectively reduced the space hold area, and the condition to the laboratory is no longer overcritical, more can be under the demand of extensive work, set up simultaneously many group Multi-level collection filter for biological samples, accelerate the progress of work.
5. the present invention can with used different size filter membrane, realize sample water throughput to greatest extent.Not only effectively reduce the required time of water sample that gathers.Also guarantee the homogeneity of sample, can provide good support for follow-up work.
6. the present invention has increased the electronic water level monitoring, make experimental data obtain to be more prone to, more accurate.
7. the present invention can extensively adapt to the requirement of experiment of different subjects, as long as carry out simple function adjustment, can become acquisition platform wide in range, the scientific experiment data splitting.
Description of drawings
Fig. 1 is automatic multi-layer level biological sample collection filter system single group on-line operation schematic diagram;
Fig. 2 is automatic multi-layer level biological sample collection filter nested design connection work schematic diagram;
Fig. 3 is the Multi-level collection filter for biological sample schematic diagram;
Fig. 4 is that the present invention collects filter cylinder front elevation and vertical view;
Fig. 5 is filter membrane clamping kit schematic diagram of the present invention;
Fig. 6 is apparatus of the present invention system controller schematic diagram.
Specific embodiment
As shown in Figure 3, sampling main body Multi-level collection filter for biological sample of the present invention (B) is according to primary filter by a cover collection filter cylinder (1) and filter membrane clamping kit (2), middle rank filters, the collection filter device that the order of final stage filtration (fine filtering) assembles, the connection of collection filter cylinders at different levels (1) adopts the mode that screw thread spins or hinge is fastening to connect.The placement of collection filter cylinder is not subjected to the restriction of attitude own, but generally vertical hanging is easier to satisfy the needs that use.
The present embodiment spins mode as example take screw thread: as shown in Figure 4, collection filter cylinder (1) uses the cylinder material of seawater corrosion resistance to make, there are respectively outer tooth interface (11) and interior tooth interface (12) in two ends, the seal groove ((13), (14)) that annular also is set respectively at two ends, barrel up and down can be placed " O " RunddichtringO, guarantees that the connection of collection filter cylinders at different levels (1) can not leaked gas; At the bottom of collection filter cylinder (1), the top of interior tooth interface (12), be provided with a filter membrane clamping kit groove (15), the diameter of filter membrane clamping kit groove (15) is greater than collection filter cylinder (1) internal diameter, internal diameter less than interior tooth interface (12), filter membrane clamping kit (2) is inserted in filter membrane clamping kit groove (15), consists of one-level collection filter device; As shown in Figure 5, filter membrane clamping kit (2) is comprised of upper clamping kit (21), lower clamping kit (22) and filter membrane (23); Upper clamping kit (21) adopts the hollow resistant material, and upper surface is smooth can combine closely with the filter membrane clamping kit groove (15) of collection filter cylinder (1), and lower surface is provided with groove and lower clamping kit (22) upper surface tongue closely meshes; Lower clamping kit (22) is the flat board of a densely covered micropore, and the aperture of these micropores, pitch-row all are not more than 0.5mm, and lower surface is smooth flat, bears the extruding that subordinate's collection filter cylinder (1) connects, and reaches to use air tight, watertight seal request; Filter membrane (23) installs in the center of lower clamping kit (22), upper clamping kit (21) is buckled in the draw-in groove of lower clamping kit (22), confirms that clamping kit is buckled well up and down.According to different job requirements, can use the filter membrane (23) of required different grain size level, different size specification, change different clamping kits up and down according to the dimensions of filter membrane (23) simultaneously.
With the filter membrane clamping kit (2) buckled well according to primary filter, middle rank filters, final stage is filtered the order of (fine filtering), insert successively in the filter membrane clamping kit groove (15) of collection filter cylinders at different levels (1), again collection filter cylinders at different levels (1) order is assembled, wherein the initial and end two ends are linkage section, draw pipeline by linkage section and are connected with other device, as shown in Figure 3.
The pipeline that on Multi-level collection filter for biological sample (B), linkage section picks out as shown in Figure 1 is connected with sample water container (E), the pipeline that lower linkage section is drawn is connected C with flow monitoring device) connect, flow monitoring device (C) is connected with gas and water separator (D) by pipeline, and gas and water separator (D) is connected by pipeline with vaccum-pumping equipment (A); Flow detector (C) is connected with function 1 port of system controller (F), the draining control valve (I) of moisture trap (D) is connected with function 4 ports of system controller (F), Call Transfer circuit (G) is connected with function 5 ports of system controller (F), and vaccum-pumping equipment (A) is connected with function 3 ports of system controller (F).According to the demand difference of sampling work, automatic multi-layer level biological sample collection of the present invention is filtered the Multi-level collection filter for biological sample (B) of system and can be selected the mode of single group direct (as Fig. 1) and the connection (as Fig. 2) of being connected to connect.When adopting the grouping connected mode, each is organized between collecting filter and connects by pipeline according to need of work.
After installing collection filter cylinder and various connection, at first need automatic multi-layer level biological sample collection filter system is debugged.Debug process is undertaken by system controller (F), and as shown in Figure 6, function 1 is flow monitoring, and function 2 is the water level induction monitoring, and function 3 is controlled for the vaccum-pumping equipment power supply, and function 4 is controlled for draining, and function 5 is Call Transfer.According to the different requirements of different operating to the collection capacity of water sample, set the collection capacity of water sample.Automatic multi-layer level biological sample collection of the present invention is filtered system can arrange the collection capacity of water sample in 0.5L～20L scope, press the collection capacity of the water sample of key assignments needs by the function 1 on system controller (F) panel and function 2.
After debugging is completed, can begin sampling work.Press function 3 buttons on lower panel, start vaccum-pumping equipment (A), system starts working.The course of work that automatic multi-layer level biological sample collection of the present invention is filtered system need not manually on duty, after collection capacity that flow monitoring device (C) detects the water sample in moisture trap (D) reaches default value, system controller (F) sends the cut-out instruction, cut off the power supply of vaccum-pumping equipment (A), stop current work, and (G) sends the signal of ringing by the Call Transfer device, comrade staff changes water sample and continues or finish sampling work, and the time of the signal of ringing is 10 seconds.In the signal ended 30 seconds of ringing, continue the intervention of operation as prosthetic, system controller (F) will be controlled Call Transfer device (G) and send wireless paging signals, and the entrained radio receiver of staff can receive signal.Confirm to press function 4 buttons on lower panel after inferior sampling work is completed the staff, complete sample water after filtration in the separator of dropping a hint (D) enters in the Job readiness of next stage.
For the fault that prevents flow monitoring device (C) causes adverse effect to work, the present invention also is provided with a cover water level monitoring induction installation (H).This device inductor is connected with function 2 ports of system controller (F) in being located at moisture trap (D), can require the water sampling greater than 1L to set.After reaching the collection capacity requirement, this inductor also can pass through the power supply that system controller (F) cuts off vaccum-pumping equipment (A).Stop current work and send signal, notifying the staff to change water sample and continue or finish sampling work.
Claims (10)
1. an automatic multi-layer level biological sample collection is filtered system, it is characterized in that:
The inlet and outlet pipe that Multi-level collection filter for biological sample (B) two ends pick out is connected C with sample water container (E) respectively with flow monitoring device) be connected, flow monitoring device (C) is connected with gas and water separator (D) by pipeline, and gas and water separator (D) is connected by pipeline with vaccum-pumping equipment (A); The draining control valve (I) of flow detector (C), moisture trap (D), Call Transfer device (G) and vaccum-pumping equipment (A) are connected with system controller (F) respectively;
Described Multi-level collection filter for biological sample (B) be by a cover collection filter cylinder (1) and filter membrane clamping kit (2) formation according to primary filter, middle rank filters, the collection filter device that the order of final stage filtration (fine filtering) assembles, its two ends are upper and lower linkage section, draw respectively inlet and outlet pipe; The bottom of collection filter cylinder (1) is provided with a filter membrane clamping kit groove (15), inserts filter membrane clamping kit (2) in filter membrane clamping kit groove (15).
2. automatic multi-layer level biological sample collection according to claim 1 is filtered system, it is characterized in that: described collection filter cylinder (1) uses the cylinder material of seawater corrosion resistance to make, there are respectively outer tooth interface (11) and interior tooth interface (12) in two ends, the seal groove ((13), (14)) that annular also is set respectively at two ends, barrel up and down can be placed " O " RunddichtringO, and collection filter cylinder (1) adopts screw thread to spin fastening by outer tooth interface (11) and interior tooth interface (12).
3. automatic multi-layer level biological sample collection according to claim 1 and 2 is filtered system, it is characterized in that: the mode that described collection filter cylinder (1) can also adopt hinge to compress connects.
4. automatic multi-layer level biological sample collection according to claim 1 is filtered system, and it is characterized in that: described filter membrane clamping kit (2) is comprised of upper clamping kit (21), lower clamping kit (22) and filter membrane (23); Upper clamping kit (21) adopts the hollow resistant material, and upper surface is smooth can combine closely with the filter membrane clamping kit groove (15) of collection filter cylinder (1), and lower surface is provided with groove and the engagement of lower clamping kit (22) upper surface tongue; Lower clamping kit (22) is the flat board of a densely covered micropore, and lower surface is smooth flat; Filter membrane (23) device is in the center of lower clamping kit (22).
5. according to claim 1 or 4 described automatic multi-layer level biological sample collection are filtered system, it is characterized in that: described filter membrane clamping kit (2) can be changed filter membrane and the up and down clamping kit of different size according to different purposes.
6. automatic multi-layer level biological sample collection according to claim 1 filter system is characterized in that: described Multi-level collection filter for biological sample (B) can be selected single group direct mode and more to make up the connected mode of dividing into groups and be connected E with flow monitoring device (C) with the sample water vessel) be connected.
7. automatic multi-layer level biological sample collection according to claim 1 is filtered system, and it is characterized in that: the air intake opening of described sample water container (E) is provided with air strainer.
8. automatic multi-layer level biological sample collection according to claim 1 is filtered system, it is characterized in that: also comprise the water level monitoring induction installation (H) that is connected on system controller (F) in described moisture trap (D).
9. automatic multi-layer level biological sample collection according to claim 1 is filtered system, and it is characterized in that: described system controller (F) can be sampled in the scope of 0.5L～20L, and its sampling operation step is:
A. assemble acquisition system, the water sampling amount is set;
B. start acquisition system, reach setting value when flow monitoring device (C) detects water sample, system controller (F) cuts off the power supply of vacuum extractor, and the signal of ringing simultaneously quits work;
C. in the signal ended of ringing 30 seconds, the prosthetic operation gets involved, and sends wireless paging signals.
10. automatic multi-layer level biological sample collection according to claim 1 is filtered system, it is characterized in that: described system controller (F) inside is provided with dry cell, during the external power source outage, system controller (F) passes through dry cell power supply, send the signal of ringing, send prompting to the staff.
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| CN2011103417713A CN103091147A (en) | 2011-10-28 | 2011-10-28 | Automated multilevel biological sample filtering system |
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| CN2011103417713A CN103091147A (en) | 2011-10-28 | 2011-10-28 | Automated multilevel biological sample filtering system |
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Cited By (1)
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| CN108744693A (en) * | 2018-05-23 | 2018-11-06 | 上海北裕分析仪器股份有限公司 | The portable nutsch filter of multistage filtering with sealed compartment |
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Application publication date: 20130508 |