CN103088005A - Culture medium for improving chitinase produced by streptomyces sampsonii KJ40 and chitinase bacteria-containing preparation and application - Google Patents
Culture medium for improving chitinase produced by streptomyces sampsonii KJ40 and chitinase bacteria-containing preparation and application Download PDFInfo
- Publication number
- CN103088005A CN103088005A CN2013100386106A CN201310038610A CN103088005A CN 103088005 A CN103088005 A CN 103088005A CN 2013100386106 A CN2013100386106 A CN 2013100386106A CN 201310038610 A CN201310038610 A CN 201310038610A CN 103088005 A CN103088005 A CN 103088005A
- Authority
- CN
- China
- Prior art keywords
- chitinase
- enzyme
- substratum
- culture medium
- bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
The invention discloses a culture medium for improving chitinase produced by streptomyces sampsonii KJ40 and a chitinase bacteria-containing preparation and application. The culture medium is composed of 100ml/L of colloid chitin with solubility of 2%(w/V), 0.4wt% of soybean cake powder, 1.0(g/L) of K2HPO4, and 0.6(g/L) of KH2PO4. Compared with an enzyme production basic culture medium, the activity of the chitinase produced according to new formulation and fermentation condition disclosed by the invention is raised by 80%. The chitinase bacteria-containing preparation with 50-100 times (volume ratio) dilution can be used for performing root-irrigation on breadth of root system of poplar nursery stock with 100ml for each poplar, and is respectively applied for once in spring and summer so as to prevent violet root rot of the poplar.
Description
Technical field
What the present invention relates to is that substratum and the chitinase that a kind of Sang Shi of raising streptomycete KJ40 produces chitinase contains bacteria preparation and application.
Background technology
The throughput of microbial strains only just can be not fully exerted under the most adaptable method.The production level of good microbial strains is restricted by many factors, comprises culture medium prescription, initial pH, culture temperature, inoculation condition, Oxygen supplied level etc.Fermention medium is the basic substance of microorganism growth, and it forms and proportioning has considerable influence to thalli growth, antibiotics fermentation unit, refining technique, quality product etc.The improvement of fermention medium is an important approach that improves fermentation level, must comprise the energy, element and the special nutrient that satisfy microorganism, wherein the kind of various compounds and concentration have important impact to the Quality and yield of thalli growth breeding and product.Because microbe species is different, the substratum that uses during the fermentation is also different, and the raw material of substratum mainly contains carbon source, nitrogenous source, inorganic salts, trace element and water etc. usually.Carbon source is the nutritive substance that consists of carbon element in microorganism cells and meta-bolites, generally accounts for 50% left and right of microorganism cells dry-matter.Due to the difference of various microbial physiology characteristics, its carbon source kind that can utilize is also different.Carbon source commonly used has carbohydrate, fat, organic acid and alcohol, hydrocarbon polymer etc.Nitrogenous source is the nutritive substance that consists of the nitrogen in microorganism cells and meta-bolites, is one of main raw material that uses in microbial fermentation.Some nitrogen source can participate in synthetic antimicrobial material precursor, can be to the synthetic regulating effect that plays of some special meta-bolitess
[174,175]The organic source of nitrogenous source and inorganic nitrogen-sourced two large classes commonly used, organic nitrogen source comprises soybean cake powder, cottonseed meal, groundnut meal, corn steep liquor, dregs of beans, wheat bran, yeast powder, peptone, fish meal and vinasse etc.; Inorganic nitrogen-sourced have urea, ammoniacal liquor, sulfate of ammoniac, ammonia chloride and a nitrate etc.It is needed in Reproduction and product synthesize that trace element and inorganic salt are microorganisms.Metal ion is mostly relevant with its concentration on the impact of microbial physiology activity, and lower concentration is usually expressed as the stimulation inducing action, and high density shows restraining effect, and optimal concentration will be determined according to microbiological property and fermentation condition.Water is the chief component composition of substratum, and to the Reproduction of microorganism with product is synthetic that important effect arranged, it is the main component that consists of somatic cells, is again the medium that all nutritive substances transmit.In some fermentation, nutritive deficiency is the condition that antimicrobial substance begins to synthesize, so in substratum, the addition of each nutritive element is also most important to fermentative production.
In the situation that fermentative medium formula determines, another the important approach that improves fermentation level is to select suitable culture condition, comprises temperature, plants age, inoculum size, initial pH, dissolved oxygen amount and fermentation time etc.Temperature is the result of various factors general performance on the impact of thalli growth and meta-bolites formation
[177], can be by affecting dissolved oxygen impact fermentation in fermented liquid as temperature.From enzymatic reaction kinetics, temperature raises, and speed of response strengthens thereupon, and metabolism is accelerated, and product produces in advance, but thalline is easy to aging and then affects product synthesize.The inoculum size size is relevant with the growth and breeding speed in substratum with bacterial strain kind Age, and inoculum size greatly seed is easier to adapt to after entering fermentor tank, thereby shortens the thalline breeding time required to the peak, accelerates the product resultant velocity; But excessive inoculum size usually can make thalli growth too fast and cause nutraceutical matrix to lack or dissolved oxygen not enough, be unfavorable for fermentation; The too small earlier fermentation thalli growth that can make of inoculum size is slow, extends fermentation period, and the mycelia amount is few, also may form hypha body, causes fermentation abnormal.Thalline is in process of growth, and the physiologically active difference of different growth phases is larger, and the control of planting age in fermenting process is just particularly important.Usually be in the vigorous logarithmic growth middle and later periods of vitality with thalline and be advisable, the seed concentration of this moment is large, and quality is high, after accessing suitable amount, can conform quickly, is conducive to shorten fermentation period.Every kind of microorganism has the optimum pH of oneself growing, and during the fermentation, the variation of pH value can not only change the environment of system enzyme and the metabolism stream of nutritive substance, can also make inductor and somatomedin change between active and nonactive.The optimal pH scope of actinomycetes growth is 6-8, generally adds certain buffer substance to keep stable pH value in substratum.Dissolved oxygen is the another important factor that affects microbial fermentation, can route of synthesis and the resultant velocity of meta-bolites be exerted an influence
[179]Usually in the actinomycete fermentation process, cell density is crossed conference and is caused the fermented liquid thickness, and then in the device that induces reaction, dissolved oxygen is too low, can cause again the mycelia fracture or form bead but dissolved oxygen is too high, thereby growth and the synthetic of product to thalline impact, and improve these situations so need to adjust dissolved oxygen amount.The length of fermentation time is also very important index in fermentative production, can directly affect productive rate and the quality of meta-bolites.
This research to Sang Shi streptomycete KJ40(Sang Shi streptomycete KJ40 the applicant on April 25th, 2012 with the application of bacterial classification form patent, Chinese patent application number 201210122697.0 denominations of invention: Sang Shi streptomycete KJ40 bacterial strain and liquid preparation thereof, publication number CN102703343A) fermentative medium formula of product chitinase is optimized, to can significantly improving bacterial strain enzymic activity content.Improve prevention effect.
Chitinase is one of important mechanisms of biocontrol microorganisms inhibition pathogenic fungi as a kind of antibacterial protein.Suitable medium composition and culture condition are essential for improving chitinase output, and carbon source, nitrogenous source, pH value etc. are all closely related with chitinases produced by microbes.Therefore people are when improving the product chitinase amount of microorganism, must carry out appropriate regulation to fermentation condition, to determine the suitableeest condition of enzyme production of this bacterium, spore germination and mycelial growth for stronger Antifungi, excite plant to start defensive raction, make plant produce more Buchner's bodies, the resistance that strengthens plant provides the basis.
Summary of the invention
Technical problem to be solved by this invention is to provide substratum that a kind of Sang Shi of raising streptomycete KJ40 produces chitinase and chitinase to contain the application that bacteria preparation and chitinase contain bacteria preparation for the deficiencies in the prior art.
Sang Shi streptomycete KJ40 the applicant in the present invention on April 25th, 2012 with the application of bacterial classification form patent, Chinese patent application number 201210122697.0 denominations of invention: Sang Shi streptomycete KJ40 bacterial strain and liquid preparation thereof, publication number CN102703343A.
Technical scheme of the present invention is as follows:
A kind ofly improve the substratum that Sang Shi streptomycete KJ40 produces chitinase, described substratum is: solubility is 2%(w/V) tobacco brown spot pathogen 100ml/L, soybean cake powder 0.4wt%, K
2HPO
41.0 (g/L), KH
2PO
40.6 (g/L).
The chitinase of described substratum preparation contains bacteria preparation, in the described substratum of described Sang Shi streptomycete KJ40 seed access, then is placed on constant-temperature table, and in 26 ℃, 140r/min shaking culture 144 hours is made chitinase and contained bacteria preparation.
Described chitinase contains the application of bacteria preparation in making prevention willow riolet root rot medicament, described chitinase is contained bacteria preparation doubly dilute according to volume ratio 50-100, fills with root along seedlings of poplar root system breadth, 100 milliliters of every strains, and annual spring, summer execute each and use once.
With product enzyme basic medium (tobacco brown spot pathogen 200ml, K
2HPO
40.7g, KH
2PO
40.3g, M gSO
47H
2O0.5g, FeSO
47H
2O0.01g, ZnSO
40.001g, distilled water 1000ml, pH7.0) to compare, the chitinase that new formula of the present invention and fermentation condition are produced is active improves approximately 80%.Contain bacteria preparation 50-100 times of (volume ratio) extent of dilution with above-mentioned chitinase and fill with root along seedlings of poplar root system breadth, 100 milliliters of every strains, annual spring, summer are executed each with once, can prevent the generation of willow riolet root rot.
Description of drawings
Fig. 1 is the impact that fermentation time is lived on enzyme;
Fig. 2 is that different carbon sources are on the impact of enzymatic production;
Fig. 3 is that nitrogenous source is on the impact of enzymatic production;
Fig. 4 is the impact that initial pH value is produced chitinase to bacterial strain 112903;
Fig. 5 is that temperature is on the impact of inulinase-producing activity;
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Producing on the enzyme basic medium, by add carbon source, nitrogenous source, etc. experiment of single factor, determine the composition of substratum, design the orthogonal experiment of 4 factor 3 levels, the acquisition bacterial strain produces enzyme under the impact of 4 factors best proportioning is: (solubility of the tobacco brown spot pathogen aqueous solution is 2% to tobacco brown spot pathogen, w/V) 100ml/L, soybean cake powder 0.4%, K
2HPO
41.0 (g/L), KH
2PO
40.6 (g/L).Being wherein nitrogenous source content to what produce that enzyme has the greatest impact, is secondly K
2HPO
4Content, concentration of substrate and KH by contrast
2PO
4Content is less on the impact of producing enzyme.
1.1 incubation time and the relation of producing enzyme
To produce enzyme basic medium (tobacco brown spot pathogen 200ml, K
2HPO
40.7g, KH
2PO
40.3g, M gSO
47H
2O0.5g, FeSO
47H
2O0.01g, ZnSO
40.001g, distilled water 1000ml, pH7.0) and be initial medium.The substratum that configures is divided be filled in the 100ml triangular flask, every bottle of 20ml, 121 ℃ of sterilization 30min, it is 10 that cooling rear every bottle graft enters 1m concentration
7The spore suspension of/ml, each parallel sample are established 3 repetitions, then be placed on constant-temperature table, and in 25 ℃, 140r/min shaking culture, chitinase activity of every 24 hours mensuration.Measuring method is with reference to the DNS method.Draw the product enzyme curve of biological and ecological methods to prevent plant disease, pests, and erosion streptomycete.
Experimental result as shown in Figure 1, in producing the enzyme basic medium, Sang Shi streptomycete yield of enzyme in initial 2d is lower, significantly increase but live from the time endoenzyme of the 3rd day to the 6th day, and enzyme work reaches maximum in the time of the 6th day.After this with the prolongation of incubation time, in nutrient solution, the chitinase activity begins to descend.So in experiment afterwards, select the 6th day minute as best enzyme activity.
1.2 carbon source is produced the impact of chitinase on streptomycete
The various carbon sources of interpolation 1% in basic medium are take basic medium as contrast.Carbon source has following several: glucose, 2-acetylamino-2-deoxy-D-glucose, maltose, sucrose, lactose, glycerine, starch.Study various carbon sources to producing the impact of enzyme.It is 10 that every bottle graft enters 1m concentration
7The spore suspension of/ml, each parallel sample are established 3 repetitions, then be placed on constant-temperature table, and in 25 ℃, the 140r/min shaking culture.Measured chitinase activity on the 6th day.Measuring method is with reference to the DNS method.
In basic medium, with other carbon source of the tobacco brown spot pathogen of 100ml/L and 0.1% as the compounded carbons contrast experiment of fermenting, result such as Fig. 2, with glucose, starch, maltose, lactose, NAG, when adding carbon source, absorbancy reaches the ultimate value of instrument, can't estimate chitinase content wherein.Add the enzyme activity of substratum fermented liquid of sucrose and glycerine lower than with the enzyme activity of tobacco brown spot pathogen as sole carbon source, sucrose and glycerine have certain restraining effect to producing enzyme, produce the enzyme effect during as sole carbon source with tobacco brown spot pathogen best.
1.3 nitrogenous source produces the impact of chitinase on streptomycete
Add the various nitrogenous sources of 0.2wt% in basic medium, take basic medium as contrast, investigate different nitrogen sources to producing the impact of chitinase, inorganic nitrogen-sourced have (a NH
4)
2SO4, NH
4NO
3, KNO
3Organic nitrogen source has peptone (soybean cake powder), extractum carnis.
Add respectively several single organic or inorganic nitrogenous sources in basic medium, the results are shown in Figure 3, during take peptone as nitrogenous source, enzyme activity is the highest, is secondly extractum carnis; Adopt that single to produce enzyme when inorganic nitrogen-sourced all lower.
1.4 the optimization of culture medium formula
Table 1 Antagonistic Fungi (Sang Shi streptomycete) fermention medium component orthogonal experiment factor coding schedule
Table 2 Antagonistic Fungi (Sang Shi streptomycete) fermention medium component orthogonal scheme design table
According to the result of experiment of single factor, with tobacco brown spot pathogen, analysis for soybean powder content, K
2HPO
4, KH
2PO
4For producing the enzyme factor, press L9 (3
4) design three level four Factor Experiments, pH7.0,25 ℃, 140r/min carries out the shake flask fermentation test, gets respectively its crude enzyme liquid and carries out enzyme activity determination, by the SAS analysis software, determines more excellent culture medium formula.
Table 3 orthogonal experiments
According to Orthogonal experiment results, bacterial strain produces enzyme under the impact of 4 factors best proportioning is: tobacco brown spot pathogen (w/V is 2%) 100ml/L, soybean cake powder 0.4%, K
2HPO
41.0 (g/L), KH
2PO
40.6 (g/L).Being wherein nitrogenous source content to what produce that enzyme has the greatest impact, is secondly K
2HPO
4Content, concentration of substrate and KH by contrast
2PO
4Content is less on the impact of producing enzyme.
1.5 the initial p H value of substratum is produced the impact of chitinase on streptomycete
With HCl and NaOH solution, actinomycetes culture medium Initial pH is distinguished furnishing 3,4,5,6,7,8,9,10,11,12,10 gradients, take intermediate value as contrast.25 ℃, 140r/min carries out the shake flask fermentation test, gets respectively its crude enzyme liquid and carries out enzyme activity determination.
The initial p H value of substratum is adjusted to respectively 3.0～12.0, and the initial pH of research substratum produces the impact of chitinase on the Sang Shi streptomycete.Experimental result shows (as Fig. 4), and the change procedure of pH from 3.0 to 7.0, in fermented liquid, the chitin enzyme activity is the trend of increase, and is to reach maximum at 7.0 o'clock in the pH value.After this, along with PH further increases, enzyme activity continuous decrease in fermented liquid.It is neutral that the best of Sang Shi streptomycete is produced enzyme PH, and the too high or too low impact on the product enzyme of PH is larger.
As shown in Figure 4, the initial pH of culture medium is the highest at 7.0 o'clock enzyme activities, when pH is too high or too low, produces enzyme and is subject to
1.6 different culture temperature are produced the impact of chitinase on the Sang Shi streptomycete
The enzymic activity of the 6th day is put in 24 ℃, 26 ℃, 28 ℃, 30 ℃, 32 ℃ thermostat containers and cultivate, measured to substratum after optimizing respectively.
The impact that different culture temperature are produced chitinase to the Sang Shi streptomycete as shown in Figure 5.Experiment shows that the best product of this bacterial strain enzyme culture temperature is 26 ℃.At 22 ℃～26 ℃, rising along with temperature, chitinase enzyme activity in fermented liquid increases, 26 ℃ of chitinase activities reach maximum, from 26 ℃～32 ℃, along with the rising of culture temperature, chitinase activity reduces gradually, on the whole, in the measuring scope, culture temperature is less on the impact that chitinase produces enzyme.
(1) Sang Shi streptomycete seed is made
1, actication of culture: Sang Shi streptomycete KJ40 slant strains is transferred to flat board (glucose 10g, peptone 5g, NaCl5g, extractum carnis 5g, agar 20g, distilled water 1000ml, pH7.0-7.2), cultivates 4d for 25 ℃.
2, Sang Shi streptomycete seed liquid preparation: with nutrient solution (tobacco brown spot pathogen 200ml, the K that configures
2HPO
40.7g, KH
2PO
40.3g, M gSO
47H
2O0.5g, FeSO
47H
2O0.01g, ZnSO
40.001g, distilled water 1000ml, pH7.0) minute be filled in the 100ml triangular flask, every bottle of 20ml, 121 ℃ of sterilization 30min, cooling rear every bottle graft enters the spore suspension that 1m concentration is 107/ml (mulberry Salmonella), then be placed on constant-temperature table, in 26 ℃, 140r/min shaking culture 36 hours.
(2) chitinase production and enzyme biopsy are surveyed
1, make product enzyme optimum formula: (solubility of the tobacco brown spot pathogen aqueous solution is 2% to tobacco brown spot pathogen, w/V) 100ml/L, soybean cake powder 0.4%, K
2HPO
41.0 (g/L), KH
2PO
40.6 (g/L), be filled in the 500ml triangular flask every bottle of 300ml, 121 ℃ of sterilization 30min.
2, inoculation and cultivation: the every bottle graft of above-mentioned fermented liquid enters Sang Shi streptomycete seed 25ml, then is placed on constant-temperature table, and in 26 ℃, 140r/min shaking culture 144 hours is made chitinase and contained bacteria preparation.
3, storage procedures: 2 years preventive effects of 4 ℃ of cryopreservations are stable.
4, the enzyme biopsy is surveyed:
(1) drafting of 2-acetylamino-2-deoxy-D-glucose typical curve
At first prepare lmg/mL 2-acetylamino-2-deoxy-D-glucose (NAG) reference liquid, then according to the form below preparation standard operation liquid
The preparation of table 4 standard operation liquid
With each pipe mixing, put into accurately water-bath 5min of boiling water, put into immediately cold water after taking-up and be cooled to room temperature, then be settled to the 25mL scale marks with distilled water, fully vibrate, shake up.Take No. 0 pipe as contrast, press NAG concentration from low to high, in photoabsorption (OD) value of the above-mentioned 7 pipe solution of 540nm place's survey.Take NAG concentration (μ g/mL) as X-coordinate, the OD value is ordinate zou, draws out typical curve.
(2) chitinase activity is measured
Add lmL fermented liquid (nutrient solution of following different ingredients) in 0.5mL tobacco brown spot pathogen and lmL phosphate buffered saline buffer (PH7.0) system, 50 ℃ of accurate water-bath lh are with DNS
[79]The reducing sugar that the method assaying reaction produces.In above-mentioned reaction system, an enzyme unit alive (U) is defined as: under 50 ℃, per minute enzyme catalysis tobacco brown spot pathogen discharges the 1 required enzyme amount of μ g 2-acetylamino-2-deoxy-D-glucose (NAG).Boil 10min again under 100 ℃; Add 1.5mL3 after ice bath is cooling, 5-NITROSALICYLIC ACID (DNS) reagent boils 10min again under 100 ℃, cooling.After centrifugal, get supernatant liquor and survey absorbancy (D) under wavelength 540nm, and do standard control with the N-amido glucose (NA G) of concentration known.Enzyme work reaches 0.8 (μ g/mL)
(3) chitinase contains bacteria preparation to the prevention and control effect of willow riolet root rot
Contain bacteria preparation 50-100 times of (volume ratio) extent of dilution with above-mentioned chitinase and fill with root along seedlings of poplar root system breadth, 100 milliliters of every strains, annual spring, summer are executed each with once, can prevent the generation of willow riolet root rot.
1, bacteriostatic action experiment: remove by filter chitinase with 0.22 micron pore size sterilizing filter and contain thalline in bacteria preparation, acquisition chitinase liquid.Measure its bacteriostatic action with hole method (it is dull and stereotyped that ordinary method is made PDA, and after inoculation willow strike germ on Bechtop, in distance germ 4cm place punching, and Xiang Kongzhong injects approximately 0.5ml chitinase liquid with 0.5cm diameter punch tool, 25 ℃ cultivation 4 days).
Table 5 Sang Shi streptomycete (Streptomyces sampsonii) KJ40 chitinase contains bacterium system
The effect (of the present invention culture medium prescription) of agent to willow strike germ
* containing same letter in the table is that difference is not remarkable, otherwise, be significant difference
Table 6 Sang Shi streptomycete (Streptomyces sampsonii) KJ40 chitinase contains bacteria preparation the effect of willow strike germ [is produced enzyme basic medium (tobacco brown spot pathogen 200ml, K
2HPO
40.7g, KH
2PO
40.3g, M gSO
47H
2O0.5g, FeSO
47H
2O0.01g, ZnSO
40.001g,
Distilled water 1000ml, pH7.0)]
* containing same letter in the table is that difference is not remarkable, otherwise, be significant difference
2, field controling test: select the medium forest land of falling ill over the years, when transplanting seedlings of poplar (strain), with above-mentioned chitinase contain bacteria preparation by volume extent of dilution along seedlings of poplar root system breadth filling root, 100 milliliters of every strains, annual spring, summer are executed each with once, add up sickness rate autumn.
Table 7 Sang Shi streptomycete (Streptomyces sampsonii) KJ40 chitinase contains bacterium system
The prevention effect (of the present invention culture medium prescription) of agent to the willow riolet root rot
* containing same letter in the table is that difference is not remarkable, otherwise, be significant difference;
Table 8 Sang Shi streptomycete (Streptomyces sampsonii) KJ40 chitinase contains bacterium system
The prevention effect (produce enzyme basic medium) of agent to the willow riolet root rot
| Chitinase contains bacterium system | Riolet root rot occurs |
| Agent concentration | Rate (%) |
| Stoste | 2a |
| Dilute 10 times | 6a |
| Dilute 50 times | 10ab |
| Dilute 100 times | 14bc |
| Dilute 200 times | 17c |
| Dilute 400 times | 22d |
| Dilute 600 times | 26de |
| Dilute 800 times | 28e |
| Contrast | 30e |
* containing same letter in the table is that difference is not remarkable, otherwise, be significant difference;
Should be understood that, for those of ordinary skills, can be improved according to the above description or conversion, and all these improve and conversion all should belong to the protection domain of claims of the present invention.
Claims (3)
1. one kind is improved the substratum that Sang Shi streptomycete KJ40 produces chitinase, it is characterized in that, described substratum is: solubility is 2%(w/V) tobacco brown spot pathogen 100ml/L, soybean cake powder 0.4wt%, K
2HPO
41.0 (g/L), KH
2PO
40.6 (g/L).
2. the chitinase of substratum preparation according to claim 1 contains bacteria preparation, it is characterized in that, in the described substratum of described Sang Shi streptomycete KJ40 seed access, then be placed on constant-temperature table, in 26 ℃, 140r/min shaking culture 144 hours is made chitinase and is contained bacteria preparation.
3. chitinase according to claim 2 contains the application of bacteria preparation in making prevention willow riolet root rot medicament, it is characterized in that, described chitinase is contained bacteria preparation doubly to be diluted according to volume ratio 50-100, fill with root along seedlings of poplar root system breadth, 100 milliliters of every strains, annual spring, summer execute each and use once.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100386106A CN103088005A (en) | 2013-01-31 | 2013-01-31 | Culture medium for improving chitinase produced by streptomyces sampsonii KJ40 and chitinase bacteria-containing preparation and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100386106A CN103088005A (en) | 2013-01-31 | 2013-01-31 | Culture medium for improving chitinase produced by streptomyces sampsonii KJ40 and chitinase bacteria-containing preparation and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103088005A true CN103088005A (en) | 2013-05-08 |
Family
ID=48201137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013100386106A Pending CN103088005A (en) | 2013-01-31 | 2013-01-31 | Culture medium for improving chitinase produced by streptomyces sampsonii KJ40 and chitinase bacteria-containing preparation and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103088005A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103396961A (en) * | 2013-07-25 | 2013-11-20 | 四川农业大学 | Streptomyces sampsonii mutant strain MV-2 resistant to thiram as well as liquid preparation, preparation method and application thereof |
| CN111690589A (en) * | 2020-07-15 | 2020-09-22 | 云南大学 | Culture medium capable of improving activity of chitinase of hirsutella sinensis mycelia |
| WO2021148064A1 (en) | 2020-01-22 | 2021-07-29 | Ustav Makromolekularni Chemie Av Cr, V.V.I. | Biodegradable polyurethane foam, biodegradable polyurethane foam-based material for saccharide-cleaving enzyme production, method of synthesis and use thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297996A (en) * | 1999-11-25 | 2001-06-06 | 张克勤 | Microbe and the method of producing biological mematocide with the microbe |
| CN1613828A (en) * | 2004-11-08 | 2005-05-11 | 江苏省农业科学院 | Preparation of multifunctional biological organic fertilizer from poultry and livestocks excrement |
| CN101518247A (en) * | 2009-04-13 | 2009-09-02 | 东北农业大学 | Preparation method of novel herbicide sugar additive |
| CN102690761A (en) * | 2012-06-07 | 2012-09-26 | 株式会社缘织 | Culture medium and preparation method thereof, microbial material and application and preparation method thereof |
| CN102703343A (en) * | 2012-04-25 | 2012-10-03 | 四川农业大学 | Streptomyces sampsonii KJ40 strain and liquid preparations thereof |
-
2013
- 2013-01-31 CN CN2013100386106A patent/CN103088005A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297996A (en) * | 1999-11-25 | 2001-06-06 | 张克勤 | Microbe and the method of producing biological mematocide with the microbe |
| CN1613828A (en) * | 2004-11-08 | 2005-05-11 | 江苏省农业科学院 | Preparation of multifunctional biological organic fertilizer from poultry and livestocks excrement |
| CN101518247A (en) * | 2009-04-13 | 2009-09-02 | 东北农业大学 | Preparation method of novel herbicide sugar additive |
| CN102703343A (en) * | 2012-04-25 | 2012-10-03 | 四川农业大学 | Streptomyces sampsonii KJ40 strain and liquid preparations thereof |
| CN102690761A (en) * | 2012-06-07 | 2012-09-26 | 株式会社缘织 | Culture medium and preparation method thereof, microbial material and application and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 《山东大学学报(理学版)》 19890331 张玉臻等 白孢链霉菌(Streptomyces albosporeus)CT_86几丁质酶研究 98－105 1－3 第24卷, 第01期 * |
| 张玉臻等: "白孢链霉菌(Streptomyces albosporeus)CT―86几丁质酶研究", 《山东大学学报(理学版)》 * |
| 潘志强等: "Marinococcus sp？PS-1产几丁质酶发酵条件研究", 《食品与发酵工业》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103396961A (en) * | 2013-07-25 | 2013-11-20 | 四川农业大学 | Streptomyces sampsonii mutant strain MV-2 resistant to thiram as well as liquid preparation, preparation method and application thereof |
| CN103396961B (en) * | 2013-07-25 | 2015-06-17 | 四川农业大学 | Streptomyces sampsonii mutant strain MV-2 resistant to thiram as well as liquid preparation, preparation method and application thereof |
| WO2021148064A1 (en) | 2020-01-22 | 2021-07-29 | Ustav Makromolekularni Chemie Av Cr, V.V.I. | Biodegradable polyurethane foam, biodegradable polyurethane foam-based material for saccharide-cleaving enzyme production, method of synthesis and use thereof |
| CN111690589A (en) * | 2020-07-15 | 2020-09-22 | 云南大学 | Culture medium capable of improving activity of chitinase of hirsutella sinensis mycelia |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102816725B (en) | Bacillus subtilis and application thereof | |
| CN110066659A (en) | A Shi bacillus is preparing the application in acid soil improving agent | |
| CN103725630B (en) | A kind of microbiobacterial agent of antagonism potato scab | |
| CN101914447A (en) | Complex microbial inoculum 707 and preparation method and application thereof | |
| CN105110961B (en) | A kind of mushroom liquid fermentation medium and its method for producing mushroom | |
| CN102550293B (en) | Method for liquid fermentation cultivation of Agaricus bisporus strain | |
| CN103493680A (en) | Tremella liquid cultivar culture method and special culture medium for cultivar | |
| CN103329720A (en) | Application of trichoderma pseudokoningii SMF2 bacterial strains in plant growth promotion | |
| CN105505782A (en) | Optimum formulas of solid medium and cultivation material of inonotus hispidus parasitizing on Ulmus macrocarpa var.mongolica | |
| CN107047319A (en) | The method for tissue culture and root media of a kind of ginkgo | |
| CN109566269A (en) | A kind of culture medium improving cordycepin content in Cordyceps militaris | |
| CN107353127A (en) | A kind of microorganism Water soluble fertilizer for preventing and treating corn fungal disease | |
| CN103146600B (en) | Antagonistic bacteria for prevention and treatment of tobacco bacterial wilt and application thereof | |
| CN103088005A (en) | Culture medium for improving chitinase produced by streptomyces sampsonii KJ40 and chitinase bacteria-containing preparation and application | |
| CN104686583A (en) | Application of microorganism bacterium agent in resisting of potato scab | |
| CN102443559B (en) | Baclillus subtilis used for controlling cotton verticillium wilt and application thereof | |
| CN102550294B (en) | Method for liquid fermentation cultivation of Pleurotus cornucopiae strain | |
| CN105274026B (en) | A kind of silicate-dissolving microbe and its application in crop-planting | |
| CN103305449A (en) | Formula and preparation method of culture medium used for Niannianle seed-streptomyces microflavus from cultivation to fermentation | |
| CN108277167B (en) | Method for biologically preventing and treating phytophthora capsici leonian | |
| CN103005437A (en) | Method for preparing Se-enriched bran edible mushroom nutrition powder by solid cultivation | |
| CN102732435B (en) | Masson pine rhizosphere solubilizing phosphorus fungus aspergillus awamori and application thereof | |
| CN107099465B (en) | Growth-promoting bacterial strain and its microorganism seedling medium for promoting cuttage tea shoot to take root | |
| CN109486705A (en) | The pale false bacillus strain X21 of one kind and its application | |
| CN104686582A (en) | Preparation method of microorganism bacterium agent for resisting potato scab |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130508 |