CN103070332A - Anti-cariogenic bacteria saccharide composition - Google Patents
Anti-cariogenic bacteria saccharide composition Download PDFInfo
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Abstract
The present invention relates to an anti-cariogenic bacteria saccharide composition, which comprises, by weight, 5-95% of isomaltulose and 5-95% of isomaltooligosaccharide. The saccharide composition provides a significant anti-cariogenic bacteria effect in the presence of cariogenic sugars, is an ideal component for food ingredients and additives, has a dental caries prevention/anti-dental caries function, and can be applicable for various foods and products.
Description
Technical field
The present invention relates to food and commodity technical field, relate in particular to a kind of sugar composite with anti-cariogenic bacteria effect.
Background technology
Carious tooth is commonly called as " decayed tooth ", " decayed tooth ", is the high disease of the human incidence of disease.Report according to WHO
[1], the most countries in Europe, North America, South America and Australia, 35-44 year crowd's dental caries lose to be mended indexes (DMFT) and was higher than for 13.9 (the tooth number that is average every human mouth troubles dental caries is higher than 13.6); 12 years old crowd's of whole world majority state permanent teeth dental caries lose the benefit index and also are higher than 1.2; Simultaneously, although the trouble dental caries situation of developed country has downward trend, the trouble dental caries of developing country are in rising trend.Therefore, the prevention of dental caries disease is the focus that the dentistry research field is paid close attention to always.
The dental caries disease is a kind of infectious diseases that sugar causes a disease that relies on.The carbohydrate (especially sucrose) of bacterium in the bacterial plaque in can metabolism food, fermentation and acid descends facing pH, causes the mineral matters such as calcium, phosphorus in the tooth to discharge i.e. demineralization.This situation can reverse when pH rises, i.e. remineralization.So the development of dental caries disease shows as the dynamic changing process that demineralization and remineralization alternately occur always, when demineralization has accounted for leading position, the mineral matter structure of tooth will be destroyed, and causes the formation of cavity.Therefore, in the process of cariogenesis development, the carbohydrate such as bacterium, sucrose all are indispensable factors.
The bacterium that causes dental caries is the resident flora in this complicated ecological environment of oral cavity.Cariogenic bacteria mainly comprises the types such as Streptococcus oralis, oral cavity lactobacillus, oral cavity actinomyces, oral cavity Wei Rong bacterium and oral cavity Neisseria at present, its streptococcus intermedius, lactobacillus and actinomyces have confirmed in multinomial research that there are substantial connection in itself and the sick morbidity of dental caries, but the relation of Wei Rong bacterium and Neisseria and dental caries disease is still not clear
[1]Studies show that, along with the generation of dental caries disease, the quantity of Streptococcus oralis, actinomyces and lactobacillus increases, wherein outstanding being changed significantly with lactobacillus and streptococcus mutans.In the Streptococcus oralis, streptococcus mutans (also claiming Streptococcus mutans) is the cariogenic bacteria of generally acknowledging, can not only cause pit and fissure caries, root face dental caries, can also cause the shiny surface dental caries, has to cause by force dental caries power.In the lactobacillus of oral cavity, the quantity of lactobacillus acidophilus in bacterial plaque is many than other lactobacillus, and is in close relations with cariogenesis.In the actinomyces of oral cavity, actinomyces viscosus more easily is colonizated in facing than other actinomyces, and is in close relations with cariogenesis.Table 1 has shown the cariogenic potential of different bacterium to animal
[2]
Table 1 different bacterium is to the dental caries power that causes of animal
Annotate: "+" expression causes dental caries power, and "-" expression does not cause dental caries power,
The biology performances such as the cariogenicity of bacterium and its growth, product acid, synthetic exocellular polysaccharide, adhesion are closely related.At first, cariogenic bacteria is grown in the oral cavity, the sugar in the metabolism food (mainly being sucrose), and then form bacterial plaque; Secondly, the cariogenic bacteria metabolism produces organic acid, causes the tooth demineralization; The 3rd, cariogenic bacteria must stick to dental surface, forms bacterial plaque and just can cause dental caries.Cariogenic bacteria synthetic cell exo polysaccharides, mainly comprise two kinds of SG (WSG) and water-insoluble glucans (WIG), the former is the mode of bacterium energy storage, virulence to cariogenic bacteria does not have a direct impact, and the latter can promote the adhesion of bacterium and gather, participate in the composition of matrix of bacterial plague, promote bacterial plaque to form and as the biological barrier of bacterial plaque, therefore for the adhesion of cariogenic bacteria with to cause dental caries significant.Studies show that, streptococcus mutans is the strongest cariogenic bacteria of synthetic water insoluble glucan ability, and other bacterial strains are synthetic less maybe can not to be synthesized, as shown in table 2
[2]
The ability of the synthetic polysaccharide of table 2 cariogenic bacteria
Annotate: "+" expression has synthetic polysaccharide ability, the not synthetic polysaccharide ability of "-" expression
Except miscellaneous bacterium is arranged, also there are other microorganisms such as fungi, mycoplasma, protozoon in the oral cavity.Poised state between they are common is most important for keeping of health.Because cariogenic bacteria is the resident flora in the oral cavity, and is extremely important again for the balance of keeping the oral cavity micro-ecological environment, is wrong so kill cariogenic bacteria by antibacterials merely.Feasible method is to reduce cariogenic bacteria to adhere to the ability that facing forms bacterial plaque, reduces its metabolism and produces acid.That is to say, suppress the growth that cariogenic bacteria produces acid, produces the ability such as sugar and suppress to a certain extent cariogenic bacteria, be only the method for real useful and effective caries prevention.
Carbohydrate in the food, such as sucrose, glucose, maltose, fructose, lactose etc., can produce as the cariogenic bacteria metabolism substrate of acid, some Saccharide and saccharide alcohols class sweet substance then can not be utilized by the oral microorganism metabolism, can be used as sugared substitute and add in the products such as food, commodity, replacement sucrose etc. causes the carbohydrate of dental caries.These sweet substances comprise xylitol, D-sorbite, maltitol, isomalt, isomaltoketose, oligoisomaltose etc.Cause in the sweet substance of dental caries these non-(low), some sugar alcohol (especially xylitol) has been proved the ability with anti-cariogenic bacteria
[3,4], but a lot of researchs report being arranged also, sugar alcohol is edible to be reached a certain amount of and will cause gastrointestinal symptom such as diarrhoea grade
[5-8]Therefore, can only be as the food additives use of limiting the quantity of.The selected isomaltoketose of the present invention and oligoisomaltose all belong to functional oligose, not only belong to non-and cause dental caries sugar, and to the human body beneficial, the batching that can be used as in the food is used.
Isomaltoketose (Isomaltulose, hereinafter to be referred as ISO), also be palatinose (Palatinose), isomery sucrose, it is that disaccharide key with sucrose changes (1 → 6) into from (1 → 2), also irreducibility disaccharide (sucrose) is changed into disaccharides (isomery sucrose), its reproducibility is about 52% of glucose.
Have experiment in vitro to prove, isomaltoketose can not be deformed the oral microorganism such as streptococcus and be used to produce acid, so it belongs to non-(low) and causes dental caries sugar
[9]
What be different from the glycitols sweetener is that isomaltoketose can not cause the gastrointestinal symptoms such as diarrhoea, and can produce wholesome effect to human body.U.S. FDA was the raw-food material of " generally recognized as safe " (Generally Recognized as Safe, GRAS) in 2005 with ISO certification
[10], can in food production, add as required quantity-unlimitingly.Research report is arranged, Healthy People and diabetes patient behind edible 50g ISO all without any bad reaction
[11]
ISO not only has very high security, can also slowly be absorbed by human body, sustainedly and stably for human body provides energy, maintains certain level after making the blood sugar concentration rising
[12,13]There are some researches show, Healthy People is delayed 15-30min in the time of taking in blood sugar concentration behind the ISO of 50g and reach peak value than the peak value after taking in sucrose, and peak concentration is not higher than 5.8mmol/L, is starkly lower than the peak value of sucrose, and blood sugar behind 3h still a little more than baseline values
[14]That is to say, the glycemic index of ISO (Glycemic index, GI) and insulin index (Insulin index, II) are lower than sucrose.
Oligoisomaltose (Isomaltooligosaccharide, hereinafter to be referred as IMO) be that a glucose residue is transferred to the α-1 in another glucose molecule or maltose or the maltotriose equimolecular, on 6, form the sugar that 2-10 the monose such as isomaltose, Isomaltotriose, Isomaltotetraose, different maltopentaose and panose forms, these sugared general names are oligoisomaltose.
U.S. FDA was " generally recognized as safe " raw-food material (GRAS) in 2005 with the oligoisomaltose authentication, thought it not by the metabolism of oral microorganism institute, also substantially can not be utilized to the synthetic water insoluble glucan, and right and wrong (low) cause the sugar of dental caries
[15]However, at present very rare about the document of oligoisomaltose and carious tooth relation.
IMO belongs to functional oligose, is considered to useful and add in the food to enteron aisle in Japan and American-European countries, and the food ingredient that also has been used as the regulating intestinal canal microorganism in China adds various food to, especially in the infant food.Bifidobacterium in human intestine's flora is that unique a kind of endotoxin that neither produces or not again exotoxin, the beneficial microbe with many physiological functions of no pathogenicity
[16], and IMO can promote the growth of Bifidobacterium in the human body intestinal canal.Studies show that, normal adults edible IMO 13.5g every day, after continuous two weeks, the quantity of Bifidobacterium significantly increases
[17]Also there are some researches prove, IMO can not only promote the beneficial microbes such as Bifidobacterium and Bacillus acidi lactici greatly to breed after taking in human body, can also obviously suppress the growth of the spoilage organisms such as clostruidium
[18]
Chinese patent application CN101238885A discloses a kind of functional health sugar composition, and it contains the isomaltoketose of 93-95% weight and the Sucralose of 5-7% weight, the effect of blood sugar level in concrete anti-caries and the holder.Said composition can not be utilized by intraoral streptococcus mutans, can not produce insoluble glucan, and because it just is hydrolyzed into glucose and fructose at small intestine, can play the effect of blood sugar level in the holder.
Present result of study only shows that isomaltoketose and oligoisomaltose right and wrong (low) cause the sugar of dental caries, and they can not be utilized by cariogenic bacteria, therefore can not produced acid or be synthesized WIG by the cariogenic bacteria utilization by the cariogenic bacteria metabolism.But studies confirm that, whether they have the ability that suppresses cariogenic bacteria, are particularly having whether still have the ability that suppresses cariogenic bacteria when causing the dental caries carbohydrate and existing.Because under actual conditions, the sweet ingredient in the human foods can not be fully replaced by the sugar of unlikely dental caries.So, find out and both have non-(low) cariogenicity, can cause carbohydrate or its combination that suppresses the cariogenic bacteria growth, produces acid and synthetic WIG under the condition that dental caries sugar exists having again, have very important significance for caries prevention.
Summary of the invention
One object of the present invention is to provide a kind of sugar composite of anti-cariogenic bacteria, formed by isomaltoketose (ISO) and oligoisomaltose (IMO), in the presence of causing dental caries sugar, can effectively suppress the sour amount of bacterial number, product of cariogenic bacteria in the oral cavity and synthesizing of water-insoluble glucan (WIG), have stronger antibiotic effect.
According to an aspect of the present invention, a kind of sugar composite of anti-cariogenic bacteria is comprised of the oligoisomaltose (IMO) of 5～95% isomaltoketose (ISO) by weight and 5～95%; Preferably, sugar composite of the present invention is comprised of 40～90% isomaltoketose and 10～60% oligoisomaltose; More preferably, sugar composite of the present invention is comprised of 70～90% isomaltoketose and 10～30% oligoisomaltose; Most preferably, sugar composite of the present invention is comprised of 90% isomaltoketose and 10% oligoisomaltose.
In specific embodiments of the present invention, the sugar composite that is comprised of isomaltoketose and oligoisomaltose shows than independent ISO or the better anti-cariogenic bacteria effect of IMO, and 5～95% isomaltoketose (ISO) and 5～95% oligoisomaltose (IMO) can cause the growth that effectively suppress cariogenic bacteria under the condition that the dental caries carbohydrate exists, produce the synthetic of acid and WIG; In other specific embodiments of the present invention, when ISO content 50%～95% and IMO content 5%～50%, sugar composite of the present invention can significantly suppress the bacterial number of streptococcus mutans and actinomyces viscosus; When ISO content 70%～90% and IMO content 10%～30%, composition of the present invention can significantly suppress the bacterial number of lactobacillus acidophilus; When ISO content 90%～95% and IMO content 5%～10%, can significantly suppress streptococcus mutans and produce the acid amount; When ISO content 85%～95% and IMO content 5%～15%, can significantly suppress the product acid amount of actinomyces viscosus; When ISO content 35%～95% and IMO content 5%～65%, can significantly suppress lactobacillus acidophilus and produce acid; When ISO content 40%～95% and IMO content 5%～60%, it is synthetic that sugar composite of the present invention can significantly reduce water-insoluble glucan (WIG).
According to a further aspect in the invention, above-mentioned cariogenic bacteria is the main cariogenic bacteria Streptococcus oralis in oral cavity, oral cavity lactobacillus and oral cavity actinomyces preferably.
Another object of the present invention provides a kind of food ingredient or additive that contains the sugar composite of described anti-cariogenic bacteria.
Sugar composite of the present invention is added in the food as sugared substitute, can when keeping the food sugariness, form by growth and the bacterial plaque that suppresses cariogenic bacteria, and play the effect of the anti-dental caries of preventing decayed tooth.
Also can add sugar composite of the present invention to any place that needs that has as the additive with preventing decayed tooth/anti-dental caries function, for example various food and commodity, in the medicine of health products, include but not limited to: toothpaste, mouthwash, cleaning wet tissue, candy, biscuit, beverage, infant food, functional food medicine etc.
A further object of the present invention is to provide the purposes of sugar composite in the anti-dental caries goods of preparation preventing decayed tooth of described anti-cariogenic bacteria.The anti-dental caries goods of these preventing decayed tooths include but not limited to food, cleaning supplies, medicine of health products etc.
The sugar composite of anti-cariogenic bacteria of the present invention is comprised of two kinds of functional oligoses, its not only right and wrong (low) effect of causing the carbohydrate of dental caries but also having significant anti-cariogenic bacteria, particularly causing the positive effect that has anti-cariogenic bacteria in the presence of the dental caries sugar, it is the ideal composition of food ingredient and additive, function with preventing decayed tooth/anti-dental caries can be applicable to various food and goods.
The accompanying drawing summary
Fig. 1 shows that the sugar composite of the present invention of Different Weight proportioning is to the streptococcus mutans affects on the growth;
Fig. 2 shows that the sugar composite of the present invention of Different Weight proportioning is to the lactobacillus acidophilus affects on the growth;
Fig. 3 shows that the sugar composite of the present invention of Different Weight proportioning is to the actinomyces viscosus affects on the growth;
Fig. 4 shows that the sugar composite of the present invention of Different Weight proportioning produces effect of acid to streptococcus mutans;
Fig. 5 shows that the sugar composite of the present invention of Different Weight proportioning produces effect of acid to lactobacillus acidophilus;
Fig. 6 shows that the sugar composite of the present invention of Different Weight proportioning produces effect of acid to actinomyces viscosus;
Fig. 7 shows that the sugar composite of the present invention of Different Weight proportioning is on the impact of the outer water-insoluble glucan of the synthetic born of the same parents of streptococcus mutans.
The specific embodiment.
Below in conjunction with accompanying drawing, by the description to better embodiment of the present invention, describe in detail but do not limit the present invention.
Material and equipment:
1, medicine and bacterial strain
Streptococcus mutans (ATCC 25175) West China College of Stomatology Sichuan University
Lactobacillus acidophilus (ATCC 4356) CICC (Chinese industrial microorganism fungus kind preservation administrative center)
Actinomyces viscosus (ATCC 15987) West China College of Stomatology Sichuan University
Isomaltoketose (ISO) food-grade equally sweet (Beijing) Food Co., Ltd
Oligoisomaltose (IMO), food-grade, Zhengzhou food Dai Tianjiao chemical products Co., Ltd
Sucrose, glucose, NaCl, anthrone, ethyl acetate, NaOH, absolute ethyl alcohol, be purchased analyze pure;
The concentrated sulfuric acid is pure for being purchased top grade.
The TPY fluid nutrient medium forms:
Caseinhydrolysate 10.0g/L, plant peptone 5.0g/L, dusty yeast 2.0g/L, glucose 5.0g/L, Cys 0.5g/L, dipotassium hydrogen phosphate 2.0g/L, magnesium chloride 0.5g/L, zinc sulfate 0.25g/L, calcium chloride 0.15g/L, iron chloride 0.0001g/L, Tween 80 1.0g/L
PH value 6.5 ± 0.1 25 ℃
2, key instrument equipment
High speed freezing centrifuge, steam sterilizer, superclean bench, constant incubator, visible spectrophotometer, light microscope, bateria chamber, desk-top pH meter, etc.
The preparation of bacteria suspension
With the microbionation of recovery 48h in the TPY fluid nutrient medium, 37 ℃ of lower anaerobism are cultivated 18h, plate coating checking be after the pure culture with bacterium liquid at 4 ℃ of centrifugal 10min of lower 4000r/min, collect bacterium, SPSS is washed bacterium twice, be adjusted into the bacteria suspension of the absorbance A BS=1.0 of 540nm place with SPSS, for subsequent use
[19]
The preparation of the sugar composite of Different Weight proportioning
First preparation contains the TPY fluid nutrient medium of 1% glucose, and setting up pH is 7.40.According to the ISO of table 4 preparation different proportion and the biased sample of IMO, then this sample is joined in the TPY fluid nutrient medium that contains 1% glucose, make each sample final concentration reach 150mg/ml, again setting up pH is 7.40.
The ISO of table 4 Different Weight proportioning and IMO grouping
Data are processed
Experimental data is processed data with SPSS 16.0 softwares, checks relatively with ANOVA Dunnett that each prescription group and blank group have there was no significant difference, and relatively between each prescription group there was no significant difference is arranged.
[embodiment 1] sugar composite of the present invention is to the cariogenic bacteria affects on the growth
Preparation contains the ISO of each weight proportion and the sugar composite of IMO (1～21 group) and blank according to the method described above, every group of parallel 4 pipes of doing of concentration, all in bacterium liquid and 1: 10 (V/V) ratio of TPY fluid nutrient medium inoculated bacteria, cultivate 48h 37 ℃ of lower anaerobism, then carry out count of bacteria and eventually last pH mensuration
[25]Adopt the method for count of bacteria, every group of bacterium liquid behind the cultivation 48h is diluted (extension rate is decided according to the situation of bacterial growth), draw in right amount in cell counting count board respectively, place 5min, at the optical microphotograph Microscopic observation and count, examine or check bacterial growth situation under the different pharmaceutical concentration according to bacterial number, with the energy for growth of each bacterium in the blank group in contrast.The results are shown in Figure 1～Fig. 3.
Fig. 1 shows that the sugar composite of different formulations is to the streptococcus mutans affects on the growth.The 1st group the bacterial number that only contains ISO significantly is lower than the 21st group (P＜0.001) only containing IMO, bacterial number all significantly is lower than the 21st group (P＜0.05) from the 2nd group to the 19th group, and bacterial number all significantly is lower than the 21st group and the 1st group (P＜0.01) from the 2nd group to the 11st group.
Fig. 2 shows that the sugar composite of different formulations is to the lactobacillus acidophilus affects on the growth.The 1st group the bacterial number that only contains ISO is compared indifference (P＞0.05) with the 21st group that only contains IMO, bacterial number all significantly is lower than the 1st group (P＜0.05) from the 3rd group to the 13rd group, and bacterial number significantly is lower than the 21st group and the 1st group (P＜0.05) from the 3rd group to the 7th group.
Fig. 3 shows that the sugar composite of different formulations is to the actinomyces viscosus affects on the growth.The 1st group the bacterial number that only contains ISO significantly is lower than the 21st group (P＜0.001) only containing IMO, bacterial number all significantly is lower than the 21st group (P＜0.001) from the 2nd group to the 20th group, and bacterial number significantly is lower than the 21st group and the 1st group (P＜0.001) from the 2nd group to the 11st group.
No matter it is independent ISO or IMO that above result shows, or all can suppress the cariogenic bacteria growth by the sugar composite that ISO and IMO form, the sugar composite that is comprised of ISO and IMO shows than independent ISO or the better anti-cariogenic bacteria effect of IMO, 5～95% ISO and 5～95% IMO can cause the dental caries carbohydrate in the presence of effectively suppress the growth of cariogenic bacteria, and when ISO content 50%～95% and IMO content 5%～50%, sugar composite of the present invention can significantly suppress the bacterial number of streptococcus mutans and actinomyces viscosus; When ISO content 70%～90% and IMO content 10%～30%, composition of the present invention can significantly suppress the bacterial number of lactobacillus acidophilus.
[embodiment 2] sugar composite of the present invention produces effect of acid to cariogenic bacteria
With cultivate behind the 48h the sample vibration evenly, get 1ml in the 5ml small beaker, with Planar Compound determination of electrode sample pH value value, and the whole last pH of the initial pH-of changing value Δ pH[Δ pH=that calculates pH], with the acid producing ability of each bacterium in the blank group in contrast.The results are shown in Figure 4～Fig. 6.
Fig. 4 shows that the sugar composite of different formulations produces effect of acid to streptococcus mutans.The 1st group the product acid that only contains ISO significantly is lower than the 21st group (P＜0.001) only containing IMO, produce acid from the 2nd group to the 18th group and all significantly be lower than the 21st group (P＜0.01), from the 2nd group and the 3rd group produce acid all significantly be lower than the 21st group and with the 1st group of there was no significant difference.
Fig. 5 shows that the sugar composite of different formulations produces effect of acid to lactobacillus acidophilus.The 1st group the product acid that only contains ISO significantly is lower than the 21st group (P＜0.01) only containing IMO, produce the acid amount from the 2nd group to the 14th group and all significantly be lower than the 21st group (P＜0.05), and with the 1st group of there was no significant difference.
Fig. 6 shows that the sugar composite of different formulations produces effect of acid to actinomyces viscosus.The 1st group the product acid that only contains ISO significantly is lower than the 21st group (P＜0.001) only containing IMO, produce the acid amount from the 2nd group to the 20th group and all significantly be lower than the 21st group (P＜0.05), produce the acid amount from the 2nd group to the 4th group and all significantly be lower than the 21st group (P＜0.05), and with the 1st group of there was no significant difference.
Above result shows, when ISO content 90%～95% and IMO content 5%～10%, can significantly suppress streptococcus mutans and produce the acid amount; When ISO content 85%～95% and IMO content 5%～15%, can significantly suppress the product acid amount of actinomyces viscosus; When ISO content 35%～95% and IMO content 5%～65%, can significantly suppress lactobacillus acidophilus and produce acid.
[embodiment 3] sugar composite of the present invention is on the impact of the outer insoluble glucan (WIG) of cariogenic bacteria synthetic cell
1 Specification Curve of Increasing
Get respectively 1mg/ml glucose titer 1ml, 2ml, 3ml, 4ml, 5ml and are settled to scale with distilled water in the 50ml volumetric flask, be made into concentration and be respectively 20ug/ml, 40ug/ml, 60ug/ml, 80ug/ml, 100ug/ml, respectively get 1ml in 10ml according to the plug test tube in, then add in order 0.5ml anthrone ethyl acetate reagent and the 5ml concentrated sulfuric acid, fully vibration in the test tube, immediately test tube is put into boiling water bath 10min, naturally cool to room temperature after the taking-up, make reference (replacing glucose solution with 1ml distilled water) with blank, under the 620nm wavelength, survey its optical density, take optical density as ordinate, take glucose sugar content as abscissa, the drawing standard curve, and obtain the normal linearity equation.
2 sample determinations
First preparation contains the TPY fluid nutrient medium of 1% sucrose.According to the ISO of table 4 preparation different proportion and the biased sample of IMO, then this sample is joined in the TPY fluid nutrient medium that contains 1% sucrose, make each sample final concentration reach 150mg/ml, adjusting pH is 7.40.Every group of parallel 4 pipes of doing of concentration all in bacterium liquid and 1: 10 (V/V) ratio of TPY fluid nutrient medium inoculated bacteria, are cultivated 40h 37 ℃ of lower anaerobism, then carry out the mensuration of water-insoluble glucan.
The centrifugal 10min of nutrient solution 6000r/min with cultivating behind the 40h abandons supernatant, and the bacterial precipitation thing is abandoned supernatant with 3ml distilled water washing, centrifugal twice; Bacterium after the washing adds 3ml 0.5N NaOH washing, centrifugal three times, merges supernatant.Supernatant is got 1ml, adds respectively 3 times of volume absolute ethyl alcohols, and 4 ℃ of refrigerators are placed and spent the night, the centrifugal 10min of 6000r/min abandons water, and precipitation adds respectively the dissolving of 4ml 0.1N NaOH solution, get 1ml sample and strong sulfuric acid response by the method for operating of above-mentioned Specification Curve of Increasing, measure optical density
[20], calculate water-insoluble exocellular polysaccharide content according to the normal linearity equation again, with the ability of the synthetic WIG of bacterium in the blank group in contrast.The results are shown in Figure 7.
Fig. 7 shows that the sugar composite of different formulations is on the impact of the outer water-insoluble glucan of the synthetic born of the same parents of streptococcus mutans.The 1st group the output of sugar conspicuousness that only contains ISO is lower than the 21st group (P＜0.001) only containing IMO, and output of sugar all significantly is lower than the 21st group (P＜0.001) from the 2nd group to the 13rd group, and with the 1st group of there was no significant difference.Through statistical analysis, the synthetic WIG amount of all prescription group streptococcus mutanses and blank group (0
#) compared significant difference (P＜0.001).
When above result showed ISO content 40%～95% and IMO content 5%～60%, it is synthetic that sugar composite of the present invention can significantly reduce water-insoluble glucan (WIG).
Antibacterial prescription preferred
Table 5 has been enumerated than the better formula range of single component fungistatic effect, as seen the 2-20 group all has the index of at least a bacterium to be better than the IMO group of one-component, and optimum group is the 3rd group, and it suppresses the energy force rate of indices of all cariogenic bacterias that this research institute measures, and all one-component is stronger.
The preferable range of the antibacterial prescription of table 5
Annotate:
*H: refer to that the 1st group is compared the group that measured value is higher with the 21st group;
*L: refer to that the 1st group is compared the group that measured value is lower with the 21st group;
#Effective range: the scope that refers to have significant difference
Experiment by above-described embodiment as seen, the sugar composite that is comprised of ISO and IMO of the present invention can suppress growth, the product acid of the main cariogenic bacteria streptococcus mutans in oral cavity, lactobacillus acidophilus and actinomyces viscosus under sucrose or glucose existence condition, suppress simultaneously the outer water-insoluble glucan of its synthetic born of the same parents.Therefore sugar composite of the present invention not only can not produced acid and WIG by the cariogenic bacteria utilization, more can cause the growth that suppresses cariogenic bacteria in the environment of dental caries sugar and produce the acid amount in existence, significantly reduce simultaneously the synthetic of WIG, can prevent and reduce the generation of carious tooth, sugar composite of the present invention has the effect of preventing decayed tooth/anti-dental caries.
Application Example:
[embodiment 4] contain the gel candy of sugar composite of the present invention
Make gel candy according to following component:
[embodiment 5] contain the biscuit of sugar composite of the present invention
Make biscuit according to following component:
[embodiment 6] contain the oral cavity of baby spraying of sugar composite of the present invention:
Prepare the oral cavity of baby spraying according to following prescription:
[embodiment 7] contain the wet tissue that cleans the teeth of sugar composite of the present invention
Prepare the wet tissue that cleans the teeth according to following prescription: ISO, IMO, glycerine and spices are mixed in the distilled water (or ultra-pure water), behind all component dissolving mixings, the sizeable nonwoven of cutting is dipped in the mixed liquor, after nonwoven fully absorbs water, packs.
The above description of this invention does not limit the present invention, and those skilled in the art can make various changes and distortion according to specification, only otherwise break away from spirit of the present invention, all should belong to claims limited range of the present invention.
List of references
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Claims (12)
1. the sugar composite of an anti-cariogenic bacteria is by 5～95% isomaltoketose and 5～95% oligoisomaltose form by weight.
2. sugar composite claimed in claim 1 is characterized in that being comprised of 40～90% isomaltoketose and 10～60% oligoisomaltose.
3. sugar composite claimed in claim 2 is characterized in that being comprised of 70～90% isomaltoketose and 10～30% oligoisomaltose.
4. sugar composite claimed in claim 3 is characterized in that being comprised of 90% isomaltoketose and 10% oligoisomaltose.
5. sugar composite claimed in claim 1 is characterized in that being comprised of 50%～95% isomaltoketose and 5%～50% oligoisomaltose.
6. sugar composite claimed in claim 1 is characterized in that being comprised of 70%～90% isomaltoketose and 10%～30% oligoisomaltose.
7. sugar composite claimed in claim 1 is characterized in that being comprised of 90%～95% isomaltoketose and 5%～10% oligoisomaltose.
8. sugar composite claimed in claim 1 is characterized in that being comprised of 85%～95% isomaltoketose and 5%～15% oligoisomaltose.
9. sugar composite claimed in claim 1 is characterized in that being comprised of 35%～95% isomaltoketose and 5%～65% oligoisomaltose.
10. sugar composite claimed in claim 1 is characterized in that being comprised of 40%～95% isomaltoketose and 5%～60% oligoisomaltose.
11. a food ingredient or additive contain each described sugar composite of claim 1～10.
12. the purposes of each described sugar composite of claim 1～10 in preparation preventing decayed tooth and/or anti-dental caries goods.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107027945A (en) * | 2016-02-04 | 2017-08-11 | 刘宗启 | A kind of preparation method for cooking liquid glucose and the health-care preserved fruit comprising the liquid glucose |
| CN112385731A (en) * | 2020-12-05 | 2021-02-23 | 福建金百利食品实业有限公司 | Formula of non-cariogenic candy and preparation process thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3871222B2 (en) * | 2005-05-25 | 2007-01-24 | 大高酵素株式会社 | Novel oligosaccharide, food added with novel oligosaccharide, production method thereof, non-cariogenic food composition, and bifidobacteria growth composition |
| CN101238885A (en) * | 2008-01-31 | 2008-08-13 | 赵芳 | Functional health sugar composition |
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- 2012-08-15 CN CN2012102896772A patent/CN103070332A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3871222B2 (en) * | 2005-05-25 | 2007-01-24 | 大高酵素株式会社 | Novel oligosaccharide, food added with novel oligosaccharide, production method thereof, non-cariogenic food composition, and bifidobacteria growth composition |
| CN101238885A (en) * | 2008-01-31 | 2008-08-13 | 赵芳 | Functional health sugar composition |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107027945A (en) * | 2016-02-04 | 2017-08-11 | 刘宗启 | A kind of preparation method for cooking liquid glucose and the health-care preserved fruit comprising the liquid glucose |
| CN112385731A (en) * | 2020-12-05 | 2021-02-23 | 福建金百利食品实业有限公司 | Formula of non-cariogenic candy and preparation process thereof |
| CN112385731B (en) * | 2020-12-05 | 2023-08-18 | 福建金百利食品实业有限公司 | Formula of non-cariogenic candy and preparation process thereof |
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