CN103060412A - Preparation method of collagen for keeping skin elasticity - Google Patents
Preparation method of collagen for keeping skin elasticity Download PDFInfo
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- CN103060412A CN103060412A CN2013100129215A CN201310012921A CN103060412A CN 103060412 A CN103060412 A CN 103060412A CN 2013100129215 A CN2013100129215 A CN 2013100129215A CN 201310012921 A CN201310012921 A CN 201310012921A CN 103060412 A CN103060412 A CN 103060412A
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Abstract
The invention discloses a preparation method of collagen for keeping skin elasticity. The preparation method comprises the steps of pretreatment, extraction, impurity removal, salting-out desalination, enzyme hydrolysis and concentration. The collagen prepared by the method, which has high purity and can be used for keeping skin elasticity, is transparent and colorless liquid; the protein concentration of the collagen is higher than or equal to 6.95%, and the amino acid content of the collagen is higher than or equal to 2.32%; the collagen can be directly used as cosmetics; and the preparation method is simple, convenient and beneficial to scale production.
Description
Technical field
The present invention relates to a kind of collagen protein preparation method, relate in particular to the elastic collagen protein preparation method of a kind of maintenance skin.
Background technology
Elastin has reticulated structure, when applying external force, and the elastin distortion; After external force disappears, the elastin reinstatement, because this specific character, elastin is used in the skin protection cosmetics, but there are several problems in the existing elastin mode of production: the one, have variegatedly, add the outward appearance that directly affects product in the skin care product to; The 2nd, protein concn and aminoacids content are low, can not be applied directly in the skin protection cosmetics, must through reprocessing, so directly improve the price of skin protection cosmetics.
Summary of the invention
In order to solve the problems of the prior art, the purpose of this invention is to provide the elastic collagen protein preparation method of a kind of maintenance skin.
For achieving the above object, the technology used in the present invention means are:
The elastic collagen protein preparation method of a kind of maintenance skin, its step comprises:
One, pre-treatment: behind fresh bone removal fat, manadesma, after-15 ℃ of freezing sclerosis, take out, scrape off the remaining manadesma of bone surface with scalpel, then be cut into the thin slice of 0.5mm * 0.5mm along fiber direction, weighing of sheets is with abandoning liquid behind the 0.5g/L soaking in sodium carbonate solution 1h, thin slice distilled water rinsing 3 times, place Stainless Steel Wire to drain moisture on the net, dry for subsequent use;
Two, extract: the thin slice that will dry places container, extracts 48-72h with 2～3 times 0.5% acetic acid solution 0～4 ℃ of lower stirring, then uses refrigerated centrifuge with the centrifugal 8min of the speed of 3000r/min, collects supernatant liquor, is collagen protein swelling extracting solution;
Three, removal of impurities: the hydrogen peroxide that in collagen protein swelling extracting solution, adds its volume 0.5%, leave standstill 5h after mixing, next regulate the pH value with the 80g/L citric acid three sodium solution and equal 5, leave standstill in a moment and filter, abandon throw out, obtain the swelling extracting solution of remove portion foreign protein;
Four, the desalination of saltouing: under agitation in this swelling extracting solution, add an amount of sodium-chlor, slowly stir, centrifugal after leaving standstill 24h under 4 ℃ behind the 10min, collect the salt precipitation thing, be that 7.0 Tris damping fluid is at 4 ℃ of lower 24h of immersion with the pH value, recentrifuge, the collecting precipitation thing is in the dialysis tubing of packing into, after being 1.2% acetic acid solution dialysis 18h with mass concentration, use distill water dialysis 48h, what obtain at last is the higher collagen protein water swelling solution of purity again, measures wherein behind the protein content for subsequent use;
Five, enzymic hydrolysis
:The soft water of 4 times of volumes will be added in the high purity collagen water swelling solution that obtain, under agitation be warming up to 55 ℃, then be 5 with acetic acid regulator solution pH value, add papoid and carry out enzymic hydrolysis 2～4h, the papoid consumption is 1%～2% of the water-soluble swollen liquid eggs white matter quality of collagen protein, obtains Collagen Hydrolysate liquid, also can be hydrolyzed with elastoser, the elastin enzyme dosage is 0.05 %, and the pH value is 2～3, and temperature is 40 ℃;
Six, concentrated: hydrolyzed solution is carried out vacuum-concentrcted, be prepared into collagen liquid, the temperature of vacuum-concentrcted is 60～70 ℃, vacuum tightness is-0.05～-0.04MPa.Further, the gained collagen liquid is clear, colorless liquid in the described step 6, protein concn 〉=6.95%, aminoacids content 〉=2.32%.
Beneficial effect of the present invention is: the high purity by present method preparation, can be used for the elastic collagen protein of maintenance skin, be clear, colorless liquid, protein concn 〉=6.95%, aminoacids content 〉=2.32%, can be directly as cosmetic applications, and the preparation method is easy, is beneficial to scale production.
Embodiment
The invention will be further described below in conjunction with specific embodiment, to help understanding content of the present invention.
Embodiment 1
The elastic collagen protein preparation method of a kind of maintenance skin, its step comprises:
One, pre-treatment: behind fresh bone 15kg removal fat, manadesma, after-15 ℃ of freezing sclerosis, take out, scrape off the remaining manadesma of bone surface with scalpel, then be cut into the thin slice of 0.5mm * 0.5mm along fiber direction, weighing of sheets is with abandoning liquid behind the 0.5g/L soaking in sodium carbonate solution 1h, thin slice distilled water rinsing 3 times, place Stainless Steel Wire to drain moisture on the net, dry for subsequent use;
Two, extract: the thin slice that will dry places container, extracts 48h with 2 times 0.5% acetic acid solution 0 ℃ of lower stirring, then uses refrigerated centrifuge with the centrifugal 8min of the speed of 3000r/min, collects supernatant liquor, is collagen protein swelling extracting solution;
Three, removal of impurities: the hydrogen peroxide that in collagen protein swelling extracting solution, adds its volume 0.5%, leave standstill 5h after mixing, next regulate the pH value with the 80g/L citric acid three sodium solution and equal 5, leave standstill in a moment and filter, abandon throw out, obtain the swelling extracting solution of remove portion foreign protein;
Four, the desalination of saltouing: under agitation in this swelling extracting solution, add an amount of sodium-chlor, slowly stir, centrifugal after leaving standstill 24h under 4 ℃ behind the 10min, collect the salt precipitation thing, be that 7.0 Tris damping fluid is at 4 ℃ of lower 24h of immersion with the pH value, recentrifuge, the collecting precipitation thing is in the dialysis tubing of packing into, after being 1.2% acetic acid solution dialysis 18h with mass concentration, use distill water dialysis 48h, what obtain at last is the higher collagen protein water swelling solution of purity again, measures wherein behind the protein content for subsequent use;
Five, enzymic hydrolysis
:The soft water of 4 times of volumes will be added in the high purity collagen water swelling solution that obtain, under agitation be warming up to 55 ℃, then be 5 with acetic acid regulator solution pH value, add papoid and carry out enzymic hydrolysis 2h, the papoid consumption is 1% of the water-soluble swollen liquid eggs white matter quality of collagen protein, obtains Collagen Hydrolysate liquid, also can be hydrolyzed with elastoser, the elastin enzyme dosage is 0.05 %, and the pH value is 2, and temperature is 40 ℃;
Six, concentrated: hydrolyzed solution is carried out vacuum-concentrcted, be prepared into collagen liquid, the temperature of vacuum-concentrcted is 60 ℃, and vacuum tightness is-0.05MPa.
Resulting collagen liquid is clear, colorless liquid, and protein concn is 6.98%, aminoacids content 2.37%.
Embodiment 2
The elastic collagen protein preparation method of a kind of maintenance skin, its step comprises:
One, pre-treatment: behind fresh bone 20kg removal fat, manadesma, after-15 ℃ of freezing sclerosis, take out, scrape off the remaining manadesma of bone surface with scalpel, then be cut into the thin slice of 0.5mm * 0.5mm along fiber direction, weighing of sheets is with abandoning liquid behind the 0.5g/L soaking in sodium carbonate solution 1h, thin slice distilled water rinsing 3 times, place Stainless Steel Wire to drain moisture on the net, dry for subsequent use;
Two, extract: the thin slice that will dry places container, extracts 72h with 3 times 0.5% acetic acid solution 4 ℃ of lower stirrings, then uses refrigerated centrifuge with the centrifugal 8min of the speed of 3000r/min, collects supernatant liquor, is collagen protein swelling extracting solution;
Three, removal of impurities: the hydrogen peroxide that in collagen protein swelling extracting solution, adds its volume 0.5%, leave standstill 5h after mixing, next regulate the pH value with the 80g/L citric acid three sodium solution and equal 5, leave standstill in a moment and filter, abandon throw out, obtain the swelling extracting solution of remove portion foreign protein;
Four, the desalination of saltouing: under agitation in this swelling extracting solution, add an amount of sodium-chlor, slowly stir, centrifugal after leaving standstill 24h under 4 ℃ behind the 10min, collect the salt precipitation thing, be that 7.0 Tris damping fluid is at 4 ℃ of lower 24h of immersion with the pH value, recentrifuge, the collecting precipitation thing is in the dialysis tubing of packing into, after being 1.2% acetic acid solution dialysis 18h with mass concentration, use distill water dialysis 48h, what obtain at last is the higher collagen protein water swelling solution of purity again, measures wherein behind the protein content for subsequent use;
Five, enzymic hydrolysis
:The soft water of 4 times of volumes will be added in the high purity collagen water swelling solution that obtain, under agitation be warming up to 55 ℃, then be 5 with acetic acid regulator solution pH value, add papoid and carry out enzymic hydrolysis 4h, the papoid consumption is 2% of the water-soluble swollen liquid eggs white matter quality of collagen protein, obtains Collagen Hydrolysate liquid, also can be hydrolyzed with elastoser, the elastin enzyme dosage is 0.05 %, and the pH value is 3, and temperature is 40 ℃;
Six, concentrated: hydrolyzed solution is carried out vacuum-concentrcted, be prepared into collagen liquid, the temperature of vacuum-concentrcted is 70 ℃, and vacuum tightness is-0.04MPa.Resulting collagen liquid is clear, colorless liquid, and protein concn is 7.05%, and aminoacids content is 2.40%.
Embodiments of the invention are explained, and are not limited to this, anyly are familiar with those skilled in the art in the technical scope that the present invention discloses, and can expect easily changing or replacing, and all should be encompassed within protection scope of the present invention.
Claims (2)
1. one kind keeps the elastic collagen protein preparation method of skin, it is characterized in that, step comprises:
One, pre-treatment: behind fresh bone removal fat, manadesma, after-15 ℃ of freezing sclerosis, take out, scrape off the remaining manadesma of bone surface with scalpel, then be cut into the thin slice of 0.5mm * 0.5mm along fiber direction, weighing of sheets is with abandoning liquid behind the 0.5g/L soaking in sodium carbonate solution 1h, thin slice distilled water rinsing 3 times, place Stainless Steel Wire to drain moisture on the net, dry for subsequent use;
Two, extract: the thin slice that will dry places container, extracts 48-72h with 2～3 times 0.5% acetic acid solution 0～4 ℃ of lower stirring, then uses refrigerated centrifuge with the centrifugal 8min of the speed of 3000r/min, collects supernatant liquor, is collagen protein swelling extracting solution;
Three, removal of impurities: the hydrogen peroxide that in collagen protein swelling extracting solution, adds its volume 0.5%, leave standstill 5h after mixing, next regulate the pH value with the 80g/L citric acid three sodium solution and equal 5, leave standstill in a moment and filter, abandon throw out, obtain the swelling extracting solution of remove portion foreign protein;
Four, the desalination of saltouing: under agitation in this swelling extracting solution, add an amount of sodium-chlor, slowly stir, centrifugal after leaving standstill 24h under 4 ℃ behind the 10min, collect the salt precipitation thing, be that 7.0 Tris damping fluid is at 4 ℃ of lower 24h of immersion with the pH value, recentrifuge, the collecting precipitation thing is in the dialysis tubing of packing into, after being 1.2% acetic acid solution dialysis 18h with mass concentration, use distill water dialysis 48h, what obtain at last is the higher collagen protein water swelling solution of purity again, measures wherein behind the protein content for subsequent use;
Five, enzymic hydrolysis
:The soft water of 4 times of volumes will be added in the high purity collagen water swelling solution that obtain, under agitation be warming up to 55 ℃, then be 5 with acetic acid regulator solution pH value, add papoid and carry out enzymic hydrolysis 2～4h, the papoid consumption is 1%～2% of the water-soluble swollen liquid eggs white matter quality of collagen protein, obtains Collagen Hydrolysate liquid, also can be hydrolyzed with elastoser, the elastin enzyme dosage is 0.05 %, and the pH value is 2～3, and temperature is 40 ℃;
Six, concentrated: hydrolyzed solution is carried out vacuum-concentrcted, be prepared into collagen liquid, the temperature of vacuum-concentrcted is 60～70 ℃, vacuum tightness is-0.05～-0.04MPa.
2. according to the elastic collagen protein preparation method of claim 1 described maintenance skin, it is characterized in that: the gained collagen liquid is clear, colorless liquid in the described step 6, protein concn 〉=6.95%, aminoacids content 〉=2.32%.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101200497A (en) * | 2007-12-21 | 2008-06-18 | 张丽丽 | Jellyfish derived kindIIcollagen and preparation technique thereof |
| CN101363040A (en) * | 2008-09-19 | 2009-02-11 | 无锡贝迪生物工程有限公司 | Method for preparing collagen protein |
| CN102131403A (en) * | 2009-07-27 | 2011-07-20 | 成功大学 | Preparation of high purity collagen |
| CN102718832A (en) * | 2012-03-05 | 2012-10-10 | 蒋鹏 | Marine organism collagen peptide |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101200497A (en) * | 2007-12-21 | 2008-06-18 | 张丽丽 | Jellyfish derived kindIIcollagen and preparation technique thereof |
| CN101363040A (en) * | 2008-09-19 | 2009-02-11 | 无锡贝迪生物工程有限公司 | Method for preparing collagen protein |
| CN102131403A (en) * | 2009-07-27 | 2011-07-20 | 成功大学 | Preparation of high purity collagen |
| CN102718832A (en) * | 2012-03-05 | 2012-10-10 | 蒋鹏 | Marine organism collagen peptide |
Non-Patent Citations (1)
| Title |
|---|
| 汤克勇: "《胶原物理与化学》", 31 January 2012, 科学出版社 * |
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Application publication date: 20130424 |