CN103053687A - Organic bovine colostrum powder and preparation method thereof - Google Patents
Organic bovine colostrum powder and preparation method thereof Download PDFInfo
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- CN103053687A CN103053687A CN2013100109527A CN201310010952A CN103053687A CN 103053687 A CN103053687 A CN 103053687A CN 2013100109527 A CN2013100109527 A CN 2013100109527A CN 201310010952 A CN201310010952 A CN 201310010952A CN 103053687 A CN103053687 A CN 103053687A
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Abstract
The invention discloses organic bovine colostrum powder and a preparation method of the organic bovine colostrum powder. According to the preparation method, procedures of low-temperature freezing and drying and high-temperature powder ejection in the ordinary bovine colostrum powder production process are avoided; and in the bovine colostrum powder preparation procedures, ultrafiltration concentration dehydration and gamma-ray radiation sterilization are adopted. By utilizing the production process, both the purpose of dehydration concentration is achieved and the activation of immune globulin is not reduced; and meanwhile the gamma-ray radiation sterilization ensures complete nutrition components and ensures the thoroughness in sterilization. Clinical tests show that when compared with bovine colostrum powder prepared by using the conventional high-temperature powder ejection method, the bovine colostrum powder disclosed by the invention is high in IgG content and excellent in physicochemical indexes of protein, fat, moisture, acidity and various microbiological indicators.
Description
Technical field
The invention belongs to the preparing technical field of health products, particularly a kind of organic bovine colostrum and preparation technology thereof.
Background technology
Conventional bovine colostrum production technology adopts frozen drying or the high temperature link of dusting, too high or the overlong time of sterilising temp often causes the immunoglobulin (Ig) deactivation phenomenom easily, so that immunoglobulin content is on the low side in the bovine colostrum of final production, nutritive value is not high.
Summary of the invention
The object of the invention is to provide a kind of organic bovine colostrum; Another object of the present invention is to provide the preparation technology of the organic bovine colostrum of this kind.
The present invention seeks to be achieved through the following technical solutions:
A kind of organic bovine colostrum, by the following method preparation: prepare the dehydration of employing ultrafiltration concentration and gamma-radiation irradiation sterilization in the link at bovine colostrum.
A kind of organic bovine colostrum, the ultrafiltration concentration dehydration refers in its preparation method: ultrafilter filter membrane aperture is 0.1-0.3 μ m, and the ultrafiltration standard is for per hour concentrating the following liquid 8-12kg of molecular weight 30,000 dalton.
A kind of organic bovine colostrum, the ultrafiltration concentration dehydration refers in its preparation method: ultrafilter adopts German Sai Duolisi cross-flow ultrafiltration machine, and the filter membrane aperture is 0.2 μ m, and the ultrafiltration concentration standard is for per hour concentrating the following liquid 10kg of molecular weight 30,000 dalton.
A kind of organic bovine colostrum, the gamma-radiation irradiation sterilization refers in its preparation method: dose of radiation is 6-8kGy.
A kind of organic bovine colostrum, preferably by the following method successively preparation: raw material is on the waiting list; High speed centrifugation degreasing impurity elimination; The ultrafiltration concentration dehydration; Dry powder process; Ultramicro grinding; Gamma-radiation irradiation sterilization and getting.
A kind of organic bovine colostrum, preferably by the following method preparation before ultrafiltration concentration dehydration: choose endocrine healthy colostrum on the 3rd from the healthy cow farrowing, and at once be stored under the cryogenic conditions colostrum of collecting for subsequent use; The colostrum raw material of collecting is carried out the centrifugal degreasing impurity elimination in high speed low temperature centrifugal machine.
A kind of organic bovine colostrum, colostrum described in its preparation method, between the pH value 6.2～6.5, relative density is that solid content is more than 12% between 1.030 ~ 1.060.
A kind of organic bovine colostrum, cryogenic conditions described in its preparation method refer to that temperature is 0-4 ℃.
A kind of organic bovine colostrum, parameter of noncentricity described in its preparation method are 6000-7000r/min, centrifugation time 15-20min.
A kind of organic bovine colostrum, parameter of noncentricity described in its preparation method are 6000-7000r/min, 4 ℃ of temperature, centrifugation time 15-20min.
A kind of organic bovine colostrum, preferably by the following method preparation before the gamma-radiation irradiation sterilization after the ultrafiltration concentration dehydration: the concentrated colostrum that ultrafiltration is good places special-purpose tray, pallet is put into air dry oven carry out air-dry; The wafer that drying is good carries out ultramicro grinding with micronizer.
A kind of organic bovine colostrum, the drying box temperature setting is set to 50-55 ℃ in its preparation method, time 36-48h.
A kind of organic bovine colostrum, the colostrum powder granular size in its preparation method after the ultramicro grinding is more than 80 orders.
Above-mentioned organic bovine colostrum does not add any auxiliary material according to conventional method, makes capsule.
Above-mentioned organic bovine colostrum, the preparation method of its capsule is preferably: with capsule filling machine can colostrum powder, capsule standard is 0.5g/ grain ± 10mg; Capsule after the can is made capsule board via flat Autoblisterpackagingmachine after the polishing machine polishing, check that screening indefectible rear mounted box makes finished product.
Above-mentioned organic bovine colostrum according to conventional method, is made mixture, concentrated pill, capsule, pill, granule, tablet, pill, soft capsule, sustained release agent, oral liquid or the freeze drying powder injection of clinical acceptance.
Above-mentioned organic bovine colostrum and preparation thereof are for the preparation of the colostrum health products.
The organic fresh colostrum in ten thousand international pastures, the ancient riverbed of the Yellow River is selected in the organic bovine colostrum milk of the present invention source; Preparation technology adopts ultrafiltration concentration dehydration and gamma-radiation irradiation sterilization.Ultra-filtration process among the organic bovine colostrum preparation technology of the present invention not only reaches the concentrated purpose of dehydration but also can guarantee that the activity of immunoglobulin (Ig) can not be lowered, and adopts simultaneously the gamma-radiation sterilization to guarantee that namely more complete nutritional labeling has guaranteed again the completeness of sterilization.The organic bovine colostrum preparation technology of the present invention has abandoned in the conventional bovine colostrum production technology frozen drying and the high temperature link of dusting, thereby overcome immunoglobulin (Ig) deactivation phenomenom too high because of sterilising temp in the prior art or that overlong time causes, time and electric cost have greatly been saved, improved operating efficiency, and technique is brief, easy to operate and be suitable for industrialization batch production.The in addition capsule that is processed into of bovine colostrum of the present invention, instant is easy to store.
The organic bovine colostrum of the present invention shows through clinical trial, and wherein IgG content is more than 28%, and protein content is between 55-60%, and fat content is less than 1%, and moisture is less than 3%.The bovine colostrum of organic bovine colostrum of the present invention and present conventional high temperature spraying powder method preparation relatively, IgG content higher (the bovine colostrum IgG content of conventional high temperature spraying powder method preparation is between 10-15%), the physical and chemical index such as protein, fat, moisture, acidity and every microbiological indicator are all good than the latter.
Following experimental example and embodiment are used for further specifying the present invention but are not limited to the present invention
The contrast experiment of experimental example 1 every microbiological indicator
(1) concrete experimental technique and operation
Bovine colostrum to bovine colostrum and general technology high-temperature spray behind the liquid bovine colostrum before the embodiment of the invention 1 ultrafiltration, the embodiment of the invention 1 gamma-radiation irradiation sterilization carries out the detection of indices according to RHB602-2005, and concrete operation step is:
One, the mensuration of total plate count
1. the dilution of sample
1.1 colostrum capsule powder: take by weighing the 25g pulverized specimen and put in the aseptic homogeneous cup that fills 225mL physiological saline, 8000r/min～10000r/min homogeneous 1min～2min makes the sample of 1:10 and spares liquid.Former newborn sample before ultrafiltration concentration dewaters: draw the 25mL sample with aseptic straw and put in the aseptic conical flask (presetting the sterile glass beads of right quantity in the bottle) that fills 225mL physiological saline, fully mixing is made the sample of 1:10 and is spared liquid.
1.2 draw the even liquid 1mL of 1:10 sample with 1mL aseptic straw or micropipettor, slowly annotate in the sterile test tube that fills the 9mL dilution (the dilution liquid level is not touched at attention suction pipe or suction nozzle tip) along tube wall, jolting test tube or use 1 aseptic straw instead and repeatedly blow and beat it is mixed is made the even liquid of sample of 1:100.
1.3 by winding the even liquid of 10 times of series of diluted samples of preparation.Whenever increase progressively dilution once, use 1 1mL aseptic straw or suction nozzle instead.
1.4 according to the estimation to the sample contamination situation, select 2～3 the even liquid (fluid sample can comprise stoste) of suitable dilution samples, carrying out 10 times when increasing progressively dilution, draw the even liquid of 1mL sample in aseptic plate, each dilution factor is made two plates.Draw respectively simultaneously in two aseptic plates of the blank dilution adding of 1mL and make blank.
1.5 in time 15mL～20mL is cooled to 46 ℃ plate count agar culture medium (can be positioned in 46 ℃ ± 1 ℃ constant water bath box and be incubated) pour plate, and the rotation plate mixes it.
2. cultivate
After agar solidifies, with Flat plate turnover, cultivate 48h ± 2h for 36 ℃ ± 1 ℃.
3 colony countings and dull and stereotyped selection
Can detect by an unaided eye, in case of necessity with magnifying glass or colonometer, record extension rate and corresponding colony counts.Colony counting represents with CFU (colony-forming units, cfu).
3.1 choose clump count between 30cfu～300cfu, without the plate count total plate count that spreads colony growth.The flat board that is lower than 30cfu records concrete clump count, can not count greater than being recorded as of 300cfu is many.Each dilution clump count should adopt the average of two flat boards.
When 3.2 one of them flat board has larger sheet colony growth, then should not adopt, and should with without the flat board of sheet colony growth as this dilution clump count; If the sheet bacterium colony is less than half of flat board, and bacterium colony distributes very evenly in all the other half, multiply by 2 after can calculating half flat board, represents a flat-plate bacterial colony number.
3.3 when the chain-like growth that occurs on the flat board between bacterium colony without obvious boundary line, then with every strand as a colony counting.
4 results and report
4.1 the computational methods of total plate count
If 4.1.1 only have a clump count on the dilution factor flat board in suitable count range, calculate the mean value of two flat-plate bacterial colony numbers, again mean value be multiply by corresponding extension rate, as every g(mL) total plate count result in the sample.
If 4.1.2 the flat-plate bacterial colony number that two serial dilution degree are arranged in suitable count range time the, by formula N=∑ C/(n1+0.1n2) d calculates, in the formula: N---clump count in the sample, C---dull and stereotyped (flat board that contains the optimum range clump count) clump count sum; The dull and stereotyped number of n1---the first dilution factor (low extension rate); The dull and stereotyped number of n2---the second dilution factor (highly diluted multiple); D---dilution gfactor (the first dilution factor).
If 4.1.3 the bacterium colony number average is then counted the highest flat board of dilution factor greater than 300cfu on all dilution flat boards, other flat boards can be recorded as many can not counting, the result multiply by highly diluted multiple by average clump count and calculates.
If 4.1.4 all dilution flat-plate bacterial colony number averages less than 30cfu, then should multiply by extension rate by the minimum average clump count of dilution factor and calculate.
If 4.1.5 all dilution factors (comprising fluid sample stoste) flat board all without colony growth, then calculates multiply by minimum extension rate less than 1.
If 4.1.6 all dilution flat-plate bacterial colony number averages not between 30cfu～300cfu, a part during less than 30cfu or greater than 300cfu is wherein then calculated multiply by extension rate near the average clump count of 30cfu or 300cfu.
4.2 the report of total plate count
4.2.1 clump count during less than 100cfu, by " rounding up " principle revision of the convention, is reported with integer.
4.2.2 clump count is during more than or equal to 100cfu, the 3rd bit digital is got front 2 bit digital after adopting " rounding up " principle revision of the convention, and the back is with 0 replacement units; Also can represent with 10 exponential form, by behind " rounding up " principle revision of the convention, adopt two position effective digitals.
If can't count for spreading bacterium colony 4.2.3 all flat boards are upper, report that then bacterium colony spreads.
4.2.4 if colony growth is arranged on the blank, then this time testing result is invalid.
Sampling report take cfu/g as unit 4.2.5 weigh, volume sampling report take cfu/mL as unit.
Two, colibacillary detection
The dilution of sample.
1.1 colostrum capsule: take by weighing the 25g pulverized specimen, put into the aseptic homogeneous cup that fills 225mL physiological saline, 8000r/min～10000r/min homogeneous 1min～2min makes the sample of 1:10 and spares liquid.Former breast before ultrafiltration concentrates: draw the 25mL sample with aseptic straw and put in the aseptic conical flask (presetting the sterile glass beads of right quantity in the bottle) that fills 225mL phosphate buffer or physiological saline, fully mixing is made the sample of 1:10 and is spared liquid.
1.2 the pH value of the even liquid of sample should between 6.5～7.5, be regulated with 1mol/L NaOH or 1mol/L HCl respectively in case of necessity.
1.3 draw the even liquid 1mL of 1:10 sample with 1mL aseptic straw or micropipettor, slowly inject the sterile test tube (the dilution liquid level is not touched at attention suction pipe or suction nozzle tip) of 9mL physiological saline along tube wall, jolting test tube or use 1 1mL aseptic straw instead and repeatedly blow and beat, it is mixed, make the even liquid of sample of 1:100.
1.4 according to the estimation to the sample contamination situation, press aforesaid operations, successively the even liquid of tenfold ascending series dilute sample processed.Whenever increase progressively dilution 1 time, use 1 1mL aseptic straw or suction nozzle instead.Complete to the sample inoculation from the even liquid of preparation sample, overall process must not surpass 15min.
2, just fermentation test
Each sample, select the even liquid (fluid sample can be selected stoste) of sample of 3 suitable serial dilution degree, each dilution factor inoculation 3 pipe lauryl sulfate tryptone (LST) meat soup, every pipe inoculation 1mL(such as inoculum concentration surpass 1mL, then use extra quality LST meat soup), cultivate 24h ± 2h for 36 ℃ ± 1 ℃, observe in the voltage regulator tube whether Bubble formation is arranged, 24h ± 2h aerogenesis person is recurred ferment test, then continues to be cultured to 48h ± 2h such as aerogenesis not, and the aerogenesis person is recurred the ferment test.The aerogenesis person is not that coliform is negative.
3, recurrence ferment test
Get respectively culture 1 ring with oese from the LST meat soup pipe of aerogenesis, culture transferring is cultivated 48h ± 2h for 36 ℃ ± 1 ℃ in BGLB (BGLB) pipe, observe the aerogenesis situation.The aerogenesis person counts the coliform-positive pipe.
The report of coliform most probable number (MPN)
By the positive pipe of the coliform LST that winds conclusive evidence number, retrieval MPN table is reported every 100g(mL) the MPN value of coliform in the sample.
Coliform colony counting method check problem
5.1 the dilution of sample is undertaken by 1.1.
5.2 plate count
5.2.1 choose 2～3 suitable serial dilution degree, 2 aseptic plates of each dilution factor inoculation, every ware 1mL.Getting simultaneously 1mL physiological saline adds aseptic plate and makes blank.
5.2.2 the violet red bile agar (VRBA) that in time 15mL～20mL is chilled to 46 ℃ is poured in each plate approximately.Careful rotation plate with culture medium and the abundant mixing of sample liquid, after agar solidifies, adds 3mL～4mLVRBA again and covers dull and stereotyped top layer.Upset is dull and stereotyped, places 36 ℃ ± 1 ℃ to cultivate 18h～24h.
5.3 the selection of flat-plate bacterial colony number
Choose the flat board of clump count between 15cfu～150cfu, respectively dull and stereotyped upper typical case and the suspicious coliform bacterium colony that occurs of counting.Colonies typical is aubergine, and periphery of bacterial colonies has red cholate precipitation ring, and colony diameter is 0.5mm or larger.
5.4 verification test
10 dissimilar typical cases of picking and suspicious bacterium colony from the VRBA flat board, culture transferring is cultivated 24h～48h for 36 ℃ ± 1 ℃ in BGLB meat soup pipe respectively, observes the aerogenesis situation.All BGLB meat soup pipe aerogenesis can be reported as coliform-positive.
5.5 the report of coliform plate count
Test tube ratio through turning out to be at last coliform-positive multiply by the flat-plate bacterial colony number of counting in 5.3, multiply by extension rate again, is every g(mL) coliform group count in the sample.
Three, Molds and yeasts is measured
The dilution of sample
1.1 colostrum capsule: take by weighing the 25g sample to the conical flask that fills the 225mL sterile purified water, shake well is the 1:10 dilution.Former breast before ultrafiltration is concentrated: draw the 25mL sample to the conical flask that fills the 225mL sterile distilled water (can preset the sterile glass beads of right quantity in bottle) with aseptic straw, fully mixing is made the sample of 1:10 and is spared liquid.
1.2 get the 1mL1:10 dilution inject the test tube contain the 9mL sterilized water change in addition a 1mL aseptic straw repeatedly this liquid of pressure-vaccum be the 1:100 dilution.
1.3 by winding operation sequence, prepare the even liquid of 10 times of series of diluted samples.Whenever increase progressively dilution once, use the 1mL aseptic straw instead 1 time.
1.4 according to the estimation to the sample contamination situation, select 2～3 the even liquid (fluid sample can comprise stoste) of suitable dilution samples, carry out 10 times increase progressively dilution in, each dilution factor is drawn respectively the even liquid of 1mL sample in 2 aseptic plates.Get respectively simultaneously 2 aseptic plates of 1mL sample diluting liquid adding and make blank.
Can be positioned in 46 ℃ ± 1 ℃ constant water bath box and be incubated pour plate 1.5 in time 15mL～20mL is cooled to potato-glucose-agar of 46 ℃ or rose bengal medium, and rotate plate it is mixed.
2, cultivate.After agar solidifies, flat board is inverted, cultivate 5d for 28 ℃ ± 1 ℃, observe and record.
3, colony counting.Visually observe, available magnifying glass records each extension rate and corresponding Molds and yeasts number in case of necessity.Represent with CFU (colonyforming units, cfu).Choose clump count at the flat board of 10cfu～150cfu, count respectively the Molds and yeasts number according to colonial morphology.Mould spreads many can not the counting that be recorded as of the whole flat board of growth covering.Clump count should adopt the average of two flat boards.
4, result and report
4.1 calculate the mean value of two flat-plate bacterial colony numbers, again mean value be multiply by corresponding extension rate and calculate.
If 4.2 all dull and stereotyped upper bacterium colony number averages are then counted the highest flat board of dilution factor greater than 150cfu, other flat boards can be recorded as many can not counting, the result multiply by highly diluted multiple by average clump count and calculates.
If 4.3 all dull and stereotyped upper bacterium colony number averages then should multiply by extension rate by the minimum average clump count of dilution factor and calculate less than 10cfu.
4.4 if all dilution factor flat boards all without colony growth, then calculate multiply by minimum extension rate less than 1; As be stoste, then to count less than 1.
4.5 clump count in 100 the time, by " rounding up " principle revision of the convention, adopts the report of two position effective digitals.
4.6 clump count was more than or equal to 100 o'clock, front 3 bit digital are got front 2 bit digital after adopting " rounding up " principle revision of the convention, and the back replaces figure place to represent the result with 0; Can represent with 10 exponential form that also also by " rounding up " principle revision of the convention, adopt two position effective digitals this moment.
Sampling report take cfu/g as unit 4.7 weigh, mould and/or yeast number are reported or are reported respectively in volume sampling report take cfu/mL as unit.
(2) experimental result and analysis
Table 1: every microbiological indicator testing result
The raw materials used liquid bovine colostrum of the present invention is from the automatic control Animal Raising Zone, squeeze, send process management and control strict, just reached industry standard before ultrafiltration, the every microbiological indicator of technique gained colostrum capsule of the present invention is well below industry standard, and is better than general technology high temperature powder injection process gained.
The contrast experiment of experimental example 2 every physical and chemical indexs
(1) concrete experimental technique and operation
Bovine colostrum to bovine colostrum and general technology high-temperature spray behind the liquid bovine colostrum before the embodiment of the invention 1 ultrafiltration, the embodiment of the invention 1 gamma-radiation irradiation sterilization carries out the detection of every physical and chemical index according to RHB602-2005, and concrete operation step is:
One, IgG measures
1, principle
According to the principle of high-effective affinity chromatography, Immunoglobulin IgG is connected with aglucon under the phosphate buffer condition, wash-out Immunoglobulin IgG under the glycine hydrochloride condition of pH2.5,
2, reagent
2.1 mobile phase A: pH6.5, the 0.05mol/L phosphate buffer.
2.2 Mobile phase B: pH2.5,0.05mol/L glycinate acid buffer.
2.3IgG standard stock solution: take by weighing IgG standard items (Sigma chemical company) 0.01g, with mobile phase A dissolving and be settled to 10.0mL, shake up, concentration is 1.0mg/mL,
2.4IgG standard serial solution: get IgG standard stock solution, be diluted to the standard series that contains IgG0.2,0.4,0.6,0.8,1.0mg/mL with mobile phase A.Face the time spent preparation.
3, instrument and equipment
High performance liquid chromatograph tool UV-detector and gradient elution device.
4, analytical procedure
4.1 sample pretreating: take by weighing 0.1g and be accurate to 0.001g) sample is diluted to 25.0mL with mobile phase A, shakes up, by sample introduction behind the 0.45 μ m miillpore filter.
4.2 wash post with the redistilled water of 5 times of column volumes first, use again the mobile phase A of 10 times of column volumes, balance columns, sample introduction carries out wash-out by elution program.
5, the result calculates
Calculate by following formula:
X wherein: the content of IgG in the sample, unit is gram every hectogram (g/100g); M: the quality of sample, unit is gram (g); C: the content of IgG in the test solution, unit are every milliliter (mg/ml) of milligram; The volume of v sample constant volume, unit are milliliter (ml).Result of calculation keeps two position effective digitals.
Two, protein determination
1, method summary
When heating, sulfuric acid resolves into sulfurous anhydride, water and oxygen.Organic matter is oxidized to carbon dioxide and water, and the ammoniacal nitrogen of protein and excess sulfuric acid reaction change ammonium sulfate into, and ammonium sulfate distills in alkaline solution.The ammonia that distills is absorbed with boric acid, use again the accurate solution titration of acidity scale.
2, reagent
All reagent such as unreceipted specification, all refer to analyze pure; All experimental waters such as unreceipted other requirements, all refer to three grades of water.
2.1 the concentrated sulfuric acid.
2.2 potassium sulfate.
2.3 copper sulphate.
2.4 hydrogenperoxide steam generator: volume fraction is 30%.
2.5 BAS: c(H3BO3) be 30g/L.Get 30g boric acid, be dissolved in the 1L water.
2.6 methyl red-bromocresol green mixed indicator: be 95% ethanol with volume fraction, bromocresol green and methyl red be made into respectively the ethanolic solution of 1g/L, when using in the 1g/L bromocresol green: the 1g/L methyl red is that the ratio of 5:1 is mixed.
2.7 sulfuric acid standard liquid: c(H+) be 0.0500mol/L.Get the 3mL concentrated sulfuric acid and be added in the 15mL water, wash in the 1000mL volumetric flask constant volume after the cooling.
2.8 sodium hydroxide solution, mass ratio are 400/1000.Take by weighing 400g NaOH, with the water-soluble solution of 1000mL, move in the reagent bottle after cooling.
3, instrument
Common experimental chamber instrument reaches:
3.1 kjeldahl flask: 500mL or 250mL.
3.2 decide nitrogen steam distillation device.
3.3 buret: 25mL.
3.4 conical flask: 250mL.
4, operating procedure
4.1 the preparation of sample
The whole immigrations of sample are doubled approximately in the clean dried container of sample volume, cover tightly immediately container, rotational oscillation thoroughly mixes sample repeatedly.
4.2 measure
4.2.1 take by weighing sample 2g, be accurate to 0.2mg, put into kjeldahl flask, add 10g potassium sulfate and 1g copper sulphate, measure the 200mL concentrated sulfuric acid, slowly add in the kjeldahl flask, mix.
4.2.2 the bottleneck of kjeldahl flask is put a little funnel, with low baking temperature heating (a careful bottle inner foam go out and affect result), foaming in bottle stops, a little high flame.Simultaneously, can divide and add for several times 10mL hydrogenperoxide steam generator (but the flask cooling number minute must be added later on).When the color of flask contents is gradually transformed into transparent light green, when if continuation digestion 0.5～1h(kjeldahl flask wall is stained with the carbonization grain, shake or in bottle, behind the content cooling number minute, sweep away with hydrogenperoxide steam generator, continue digestion till transparent).Then take off and make it cooling.
4.2.3 the digestive juice of clarification is carefully moved in the 100mL volumetric flask, and to wash kjeldahl flask three times, cleaning solution is incorporated in the above-mentioned volumetric flask, is diluted to scale after the cooling and shakes up.
4.2.4 draw the 25mL digestive juice in deciding the nitrogen distiller, place a 250mL conical flask that fills 50mL BAS, 3 methyl red-bromocresol green mixed indicators in the lower end of condenser, make the glass tube of condenser lower end below liquid level.The 25mL sodium hydroxide solution is added (solution should be strong basicity) in the cucurbit at leisure, rapidly that the stopper plug is good, then pass into steam and distill, steam when reaching 150mL to liquid level, propose the glass tube of condenser lower end, with distilled water flushing condenser pipe lower end, washing lotion is gathered in the BAS in the lump, allows glass tube lean against the bottle wall of conical flask, liquid outlet is more than the 200mL graduation mark, continue distillation, steam to liquid level and reach 200mL.
Annotate: will note the distillation situation during distillation, avoid the liquid foam in the bottle to go out, enter receiving flask.Firepower too a little less than, the cucurbit internal pressure lowers, then liquid can flow backwards in the receiving flask, causes the failure of an experiment.
4.2.5 till being titrated to solution and claret occurs with the sulfuric acid standard liquid, record the volume of used sulfuric acid standard liquid.Carry out simultaneously blank test, and in the result, proofreaied and correct.
5, the statement of analysis result
In the formula: V---consume the volume of sulfuric acid standard liquid during titration, mL; V0---blank test consumes the volume of sulfuric acid standard liquid, mL; C(H+)---the concentration of H+ in the sulfuric acid standard liquid, mol/L; The quality of m---sample, g; 0.014---the molal weight of nitrogen-atoms, kg/mol; F---nitrogen is scaled the coefficient of protein.Milk powder is 6.38, and pure cereals (prescription) food is 5.90, and containing nursling child's cereal (prescription) food is 6.25.
Three, fat test
1, the preparation of sample
Repeatedly rotate the molten device of sample, make sample fully mix (in case of necessity, after in the laboratory sample closed container that all immigration is large, carrying out again this operation).
2, sample part
Stir gently or the rotation shuttle, so that the combined experiments sample, sampling is placed directly in liposuction bottle or other containers immediately, is accurate to 1mg, and sampling amount is as follows:
A) high fat milk powder, whole-fat milk powder, sweetened whole milk powder and prescription emulsifiable powder: about 1g.
B) partially skimmed milk powder: about 1.5g.
C) skimmed milk powder: about 1.5g.
D) whey powder: about 1.5g.
E) buttermilk powder: about 1.5g.
F) contain nursling's cereal (prescription) food: about 1.5g.
3, blank test
Blank test and sample survey are carried out simultaneously, use same steps as and identical reagent, but the sample that has diluted with the replacement of 100mL water.
4, the preparation of fat collecting bottle
In the receiving flask (4.9) of drying, add several zeolites (4.10), put into the dry 1h of baking oven (4.4).Make receiving flask cooling (preventing dust) to the temperature (the glass receiving flask needs 1h at least, and the metal ware is 0.5h at least) of balance room.
5, measure
5.1 sample treatment
The water that adds 10mL65 ℃ ± 5 ℃ washes sample in the bead of liposuction bottle, fully mixes, until sample disperses fully, puts into circulating water and cools off.
5.2 add the 2mL ammonia solution or with the liquor ammoniae fortis of volume, in bead, fully mix with the sample that has dissolved.After adding ammoniacal liquor, should carry out next step at once.
5.3 the liposuction bottle is put into 65 ℃ ± 5 ℃ water-bath, heating 15～20min, the time and vibrate once, take out, be cooled to room temperature.Starch-containing sample does not need water-bath, can carry out following step behind the static 30s.
5.4 add 10mL ethanol, content is flowed between bead and cylinder back and forth, gentle but mix up hill and dale, avoid liquid too near bottleneck.If necessary, can add 2 Congo red solution.
5.5 add the 25mL ether, beyond the Great Wall by water saturated cork, or with other bottle stoppers of water-soaked, the liposuction bottle is remained on horizontal level, the extension of bead clips to up shakes on the mixed device, by about 100 times/min vibration flask 1min, inexcessive (avoiding forming lasting emulsion).During this period, make liquid pour bead by large ball.
Cool off 5.6 in case of necessity the liposuction bottle is placed in the flowing water, then open carefully stopper, with a small amount of mixed solvent flushing stopper and bottleneck [using wash bottle during flushing], flushing liquor is flowed in liposuction bottle or the off-the-shelf fat collecting bottle.
5.7 add the 25mL benzinum, wetting stopper (being immersed in the water) again beyond the Great Wall, 30s gently vibrates.
5.8 the liposuction bottle of jumping a queue is put into centrifuge, centrifugal 1～5min under 500～600r/min.If there is not centrifuge, then the liposuction bottle is put on the support, static at least 30min, until the clarification of upper strata liquid, and obvious and aqueous phase separation.In case of necessity, be placed on cooling liposuction bottle in the flowing water.
5.9 open carefully cork or bottle stopper, mixed solvent flushing stopper and bottleneck inwall with a small amount of flow in liposuction bottle or the fat collecting bottle flushing.If two-phase interface is lower than bead and body joint, then add at leisure entry along the bottle mural margin, make liquid level be higher than bead and body joint, so that topple over.
5.10 hold the little bulb of liposuction bottle, carefully upper strata liquid is poured into as much as possible in the off-the-shelf fat collecting bottle that contains zeolite (also can select the metal ware), avoid pouring out water layer.
5.11 outside with a small amount of mixed liquor solvent washing bottleneck, flushing liquor is collected in the fat collecting bottle.The operation of being careful is in case solvent splashes the outside of liposuction bottle.Adopt the method for distillation or evaporation, remove solvent or partial solvent in the fat collecting bottle.
5.12 in the liposuction bottle, add 5mL ethanol, with alcohol flushing bottleneck inwall, by 5.4 described mixing.
5.13 repeat above operation, carry out the extracting second time, but only use 15mL ether and 1mL benzinum, with mixed solvent flushing bottleneck inwall.
5.14 repeat 5.5～5.10 operations, carry out for the third time extracting, but only use 15mL ether and 1mL benzinum, with mixed solvent flushing bottleneck inwall.
5.15 the method for employing distillation is removed the solvent (comprising ethanol) in the receiving flask, can remove solvent with evaporation to beaker or ware.Inner with a small amount of mixed solvent flushing bottleneck before the distillation.
5.16 the baking oven that fat collecting bottle is put into 102 ℃ ± 2 ℃ heats 1h, takes out receiving flask, is cooled to the temperature (do not put into drier, but will prevent dust, glass container cools off at least 1h, and canister cools off at least 0.5h) of balance room.Weighing is accurate to 0.1mg.Do not wipe receiving flask before the weighing.With clamp receiving flask is put on the balance and (avoids variations in temperature).
5.17 repeat 5.16 operations, until twice consecutive weighings of fat collecting bottle is no more than 0.5mg, the minimum quality of record receiving flask and extract.
5.18 for whether the checking extract all dissolves, in fat collecting bottle, add the 25mL benzinum, low-grade fever, jolting is until all dissolvings of fat.If extract all is dissolved in the benzinum, then contain the poor of the final mass of receiving flask of extract and initial mass, be fat content.
5.19 if extract all is not dissolved in the benzinum, or suspect that whether extract all be fat, then uses hot benzinum elution.Allow the insoluble matter precipitation of quantity thing.Pour out carefully benzinum, do not pour out any insoluble matter, repeat this operation more than 3 times, the inside of collecting bottleneck with the benzinum flushing again.
5.20 last, with the outside that bottleneck is collected in the mixed solvent flushing, avoid solution to splash the outer wall of bottle.Fat collecting bottle is put into 102 ℃ ± 2 ℃ baking oven, heating 1h, by 5.16 and 5.17 described removal benzinums, cooling, weighing.
Get the difference of the quality that the quality and 5.19 that records in 5.17 records as the quality of fat.
6, the statement of analysis result
m
1---the fat collecting bottle that records in 5.17 and the quality of extract, g; m
2---the quality of fat collecting bottle, or having in the presence of the insoluble matter, the fat collecting bottle that records in 5.19 and the quality of insoluble matter, g; m
3---in the blank test, the quality of the extract that records in the fat collecting bottle and 5.17, g; m
4---the quality of fat collecting bottle in the blank test, or when having insoluble matter to exist is the fat collecting bottle that records in 5.19 and the quality of insoluble matter, g.Report quality mark result is accurate to 0.01%.
Four, determination of moisture
1, the preparation of sample
Sample is all moved in the drying that doubles sample volume, the bottle with cover, and rotational oscillation makes it abundant mixing (in this step, can not obtain fully uniformly sample, 2 points of apart from each other that must be in sample bottle are got two duplicate samples, parallel analysis).
2, measure
2.1 ware and lid (not being placed on the ware) are put into the baking oven of 102 ℃ of scholar 2C, and heating 1h adds a cover, and then ware is moved in people's drier, is cooled to room temperature, weighing.
2.2 about 3-5g sample is put into ware, add a cover, rapidly accurately weighing.
2.3 ware and lid (not being placed on the ware) are put in the baking oven of 102 ℃ of scholar 2'C of people, heating 3h,
2.4 add a cover, ware is moved into do in behaviour's device, be cooled to room temperature, and quickly and accurately weighing.
2.5 again ware and lid (not being placed on the ware) are put into 2 ℃ of 102 ℃ of scholars' baking oven, heating 1h.Add a cover rear immigration and do in the bath device, be cooled to room temperature, rapidly weighing.
2.6 the repetition aforesaid operations, until the difference of twice consecutive weighings quality is no more than 0.0005g.
3, the result represents
In the formula: m
1The initial mass of ware and lid behind-the adding sample, g; m
2: the less quality that twice weighing obtains after the oven dry of-sample, g; m
3The quality of-sample, g.
Five, acidity assaying
1, the preparation of sample
Sample all is moved in the clean dried container that doubles approximately sample volume (with closure), covers tightly immediately container, rotational oscillation thoroughly mixes sample repeatedly.In this operating process, should avoid sample to be exposed in the air as far as possible, make the moisture of absorption reduce to minimum.
2, measure
2.1 take by weighing the 4g sample in conical flask.Accurately to 10mg.
2.2 measure the about 20 ℃ water of 96mL with graduated cylinder, make sample restore vigorous stirring, then static 20min.
2.3 in conical flask, drip sodium hydroxide solution with buret, measure with pH meter until pH reaches 8.3(), in the titration process, stir with magnetic stirring apparatus all the time, simultaneously nitrogen blowing in the conical flask prevents the airborne carbon dioxide of solution absorption.Whole titration process should be finished in 1min.Record the milliliter number of used sodium hydroxide solution, be accurate to 0.05mL.
3, the statement of analysis result
In the formula: c---the concentration of standard solution of sodium hydroxide, mol/L; V---the milliliter number of used NaOH solution during titration, mL; M---take by weighing the quality of sample, g; W---the mass fraction of moisture in the sample, %; 12---the suitable 100mL reconstituted milk of 12g milk powder (skimmed milk powder should be 9, and fat-free comminuted whey should be 7).The result remains to behind the decimal point one.
(2) experimental result and analysis
Table 2: every physical and chemical index testing result
Wherein IgG detects the assay method according to IgG in the GB/T5009.194-2003 health food, uses the Agilent high performance liquid chromatograph to detect, and protein, fat and moisture detect according to the RHB602-2005 industry standard.Data results shows that the organic bovine colostrum capsule of the present invention has utmost point significant difference (p＜0.01) than general technology high temperature powder injection process gained bovine colostrum on IgG content, protein, fat and acidity, and is with the obvious advantage.
The embodiment of the invention all can realize the effect of above-mentioned experimental example.
Embodiment 1 capsule
Step 1: raw material is on the waiting list, and chooses endocrine colostrum on the three from the healthy cow farrowing, colostrum, and color and luster is pale yellow, and clean without assorted, pH value 6.2, relative density are 1.030, solid content 12%; And at once be stored under 0 ℃ of cryogenic conditions the colostrum of collecting for subsequent use;
Step 2: high speed centrifugation degreasing impurity elimination, the colostrum raw material of collecting is carried out the centrifugal degreasing impurity elimination in high speed temperature control centrifuge; Parameter of noncentricity is set to 6000r/min, 4 ℃ of temperature, centrifugation time 15min;
Step 3: the ultrafiltration concentration dehydration, the colostrum after the high speed centrifugation degreasing impurity elimination is carried out the ultrafiltration concentration dehydration; Ultrafilter adopts German Sai Duolisi cross-flow ultrafiltration machine, and the filter membrane aperture is 0.2 μ m, and the ultrafiltration standard is 10kg per hour;
Step 4: dry powder process, the concentrated colostrum that ultrafiltration is good places dry purpose-made pallet, pallet is put into air dry oven carry out air-dry; The drying box temperature setting is set to 50 ℃, processing time 48h;
Step 5: ultramicro grinding, the wafer that drying is good carries out ultramicro grinding with micronizer; Colostrum powder granular size after the ultramicro grinding is more than 80 orders;
Step 6: the gamma-radiation irradiation sterilization, carry out the gamma-radiation irradiation sterilization with bovine colostrum; Dose of radiation is 6kGy;
Step 7: the can capsule, with capsule filling machine can colostrum powder, capsule standard is 0.5g/ grain ± 10mg; Capsule after the can is made capsule board via flat Autoblisterpackagingmachine after the polishing machine polishing, check that screening indefectible rear mounted box makes the finished product warehousing.
Embodiment 2 capsules
Step 1: raw material is on the waiting list, and chooses endocrine colostrum on the three from the healthy cow farrowing, colostrum, and color and luster is pale yellow, and clean without assorted, pH value 6.5, relative density are 1.060, solid content 12.5%; And at once be stored under 2 ℃ of cryogenic conditions the colostrum of collecting for subsequent use;
Step 2: high speed centrifugation degreasing impurity elimination, the colostrum raw material of collecting is carried out the centrifugal degreasing impurity elimination in high speed temperature control centrifuge; Parameter of noncentricity is set to 6500r/min, 4 ℃ of temperature, centrifugation time 18min;
Step 3: the ultrafiltration concentration dehydration, the colostrum after the high speed centrifugation degreasing impurity elimination is carried out the ultrafiltration concentration dehydration; Ultrafilter adopts German Sai Duolisi cross-flow ultrafiltration machine, and the filter membrane aperture is 0.2 μ m, and the ultrafiltration standard is 10kg per hour;
Step 4: dry powder process, the concentrated colostrum that ultrafiltration is good places special-purpose tray, pallet is put into air dry oven carry out air-dry; The drying box temperature setting is set to 53 ℃, processing time 40h;
Step 5: ultramicro grinding, the wafer that drying is good carries out ultramicro grinding with micronizer; Colostrum powder granular size after the ultramicro grinding is more than 85 orders;
Step 6: the gamma-radiation irradiation sterilization, carry out gamma-radiation irradiation two-stage sterilization with bovine colostrum; Dose of radiation is 7kGy;
Step 7: the can capsule, with capsule filling machine can colostrum powder, capsule standard is 0.5g/ grain ± 10mg; Capsule after the can is made capsule board via flat Autoblisterpackagingmachine after the polishing machine polishing, check that screening indefectible rear mounted box makes the finished product warehousing.
Embodiment 3 capsules
Step 1: raw material is on the waiting list, and chooses endocrine colostrum on the three from the healthy cow farrowing, colostrum, and color and luster is pale yellow, and clean without assorted, pH value 6.5, relative density are 1.050, solid content 13%; And at once be stored under 4 ℃ of cryogenic conditions the colostrum of collecting for subsequent use;
Step 2: high speed centrifugation degreasing impurity elimination, the colostrum raw material of collecting is carried out the centrifugal degreasing impurity elimination in high speed temperature control centrifuge; Parameter of noncentricity is set to 7000r/min, 4 ℃ of temperature, centrifugation time 20min;
Step 3: the ultrafiltration concentration dehydration, the colostrum after the high speed centrifugation degreasing impurity elimination is carried out the ultrafiltration concentration dehydration; Ultrafilter adopts German Sai Duolisi cross-flow ultrafiltration machine, and the filter membrane aperture is 0.2 μ m, and the ultrafiltration standard is 10kg per hour;
Step 4: dry powder process, the concentrated colostrum that ultrafiltration is good places dry purpose-made pallet, pallet is put into air dry oven carry out air-dry; The drying box temperature setting is set to 55 ℃, processing time 36h;
Step 5: ultramicro grinding, the wafer that drying is good carries out ultramicro grinding with micronizer; Colostrum powder granular size after the ultramicro grinding is more than 90 orders;
Step 6: the gamma-radiation irradiation sterilization, carry out gamma-radiation irradiation two-stage sterilization with bovine colostrum; Dose of radiation is 8kGy;
Step 7: the can capsule, with capsule filling machine can colostrum powder, capsule standard is 0.5g/ grain ± 10mg; Capsule after the can is made capsule board via flat Autoblisterpackagingmachine after the polishing machine polishing, check that screening indefectible rear mounted box makes the finished product warehousing.
Embodiment 4 tablets
Step 1: raw material is on the waiting list, and chooses endocrine colostrum on the three from the healthy cow farrowing, colostrum, and color and luster is pale yellow, and clean without assorted, pH value 6.2, relative density are 1.030, solid content 12%; And at once be stored under 0 ℃ of cryogenic conditions the colostrum of collecting for subsequent use;
Step 2: high speed centrifugation degreasing impurity elimination, the colostrum raw material of collecting is carried out the centrifugal degreasing impurity elimination in high speed temperature control centrifuge; Parameter of noncentricity is set to 6000r/min, 4 ℃ of temperature, centrifugation time 15min;
Step 3: the ultrafiltration concentration dehydration, the colostrum after the high speed centrifugation degreasing impurity elimination is carried out the ultrafiltration concentration dehydration; Ultrafilter adopts German Sai Duolisi cross-flow ultrafiltration machine, and the filter membrane aperture is 0.2 μ m, and the ultrafiltration standard is 10kg per hour;
Step 4: dry powder process, the concentrated colostrum that ultrafiltration is good places dry purpose-made pallet, pallet is put into air dry oven carry out air-dry; The drying box temperature setting is set to 50 ℃, processing time 48h;
Step 5: ultramicro grinding, the wafer that drying is good carries out ultramicro grinding with micronizer; Colostrum powder granular size after the ultramicro grinding is more than 80 orders;
Step 6: the gamma-radiation irradiation sterilization, carry out gamma-radiation irradiation two-stage sterilization with bovine colostrum; Dose of radiation is 6kGy;
Step 7: above-mentioned organic bovine colostrum, add conventional auxiliary material, according to conventional method, make the tablet of clinical acceptance.
Embodiment 5 oral liquids
Step 1: raw material is on the waiting list, and chooses endocrine colostrum on the three from the healthy cow farrowing, colostrum, and color and luster is pale yellow, and clean without assorted, pH value 6.5, relative density are 1.060, solid content 12.5%; And at once be stored under 2 ℃ of cryogenic conditions the colostrum of collecting for subsequent use;
Step 2: high speed centrifugation degreasing impurity elimination, the colostrum raw material of collecting is carried out the centrifugal degreasing impurity elimination in high speed temperature control centrifuge; Parameter of noncentricity is set to 6500r/min, 4 ℃ of temperature, centrifugation time 18min;
Step 3: the ultrafiltration concentration dehydration, the colostrum after the high speed centrifugation degreasing impurity elimination is carried out the ultrafiltration concentration dehydration; Ultrafilter adopts German Sai Duolisi cross-flow ultrafiltration machine, and the filter membrane aperture is 0.2 μ m, and the ultrafiltration standard is 10kg per hour;
Step 4: dry powder process, the concentrated colostrum that ultrafiltration is good places special-purpose tray, pallet is put into air dry oven carry out air-dry; The drying box temperature setting is set to 53 ℃, processing time 40h;
Step 5: ultramicro grinding, the wafer that drying is good carries out ultramicro grinding with micronizer; Colostrum powder granular size after the ultramicro grinding is more than 85 orders;
Step 6: the gamma-radiation irradiation sterilization, carry out gamma-radiation irradiation two-stage sterilization with bovine colostrum; Dose of radiation is 7kGy;
Step 7: above-mentioned organic bovine colostrum, add conventional auxiliary material, according to conventional method, make the oral liquid of clinical acceptance.
Embodiment 6 granules
Step 1: raw material is on the waiting list, and chooses endocrine colostrum on the three from the healthy cow farrowing, colostrum, and color and luster is pale yellow, and clean without assorted, pH value 6.5, relative density are 1.050, solid content 13%; And at once be stored under 4 ℃ of cryogenic conditions the colostrum of collecting for subsequent use;
Step 2: high speed centrifugation degreasing impurity elimination, the colostrum raw material of collecting is carried out the centrifugal degreasing impurity elimination in high speed temperature control centrifuge; Parameter of noncentricity is set to 7000r/min, 4 ℃ of temperature, centrifugation time 20min;
Step 3: the ultrafiltration concentration dehydration, the colostrum after the high speed centrifugation degreasing impurity elimination is carried out the ultrafiltration concentration dehydration; Ultrafilter adopts German Sai Duolisi cross-flow ultrafiltration machine, and the filter membrane aperture is 0.2 μ m, and the ultrafiltration standard is 10kg per hour;
Step 4: dry powder process, the concentrated colostrum that ultrafiltration is good places dry purpose-made pallet, pallet is put into air dry oven carry out air-dry; The drying box temperature setting is set to 55 ℃, processing time 36h;
Step 5: ultramicro grinding, the wafer that drying is good carries out ultramicro grinding with micronizer; Colostrum powder granular size after the ultramicro grinding is more than 90 orders;
Step 6: the gamma-radiation irradiation sterilization, carry out gamma-radiation irradiation two-stage sterilization with bovine colostrum; Dose of radiation is 8kGy;
Step 7: above-mentioned organic bovine colostrum, add conventional auxiliary material, according to conventional method, make the granule of clinical acceptance.
The invention is not restricted to these disclosed embodiment; the present invention is with the described scope of soverlay technique scheme; and the various modification of claim scope and equivalence variation; under the prerequisite that does not depart from technical solution of the present invention, any modification or improvement that those skilled in the art that the present invention is done realize easily all belong to the present invention's scope required for protection.
Claims (11)
1. an organic bovine colostrum is characterized in that adopting following method preparation: prepare at bovine colostrum and adopt ultrafiltration concentration dehydration and gamma-radiation irradiation sterilization in the link.
2. a kind of organic bovine colostrum as claimed in claim 1, it is characterized in that the ultrafiltration concentration dehydration refers in its preparation method: ultrafilter filter membrane aperture is 0.1-0.3 μ m, and the ultrafiltration standard is for per hour concentrating the following liquid 8-12kg of molecular weight 30,000 dalton; The gamma-radiation irradiation sterilization refers to: dose of radiation is 6-8kGy.
3. a kind of organic bovine colostrum as claimed in claim 1 or 2, it is characterized in that the ultrafiltration concentration dehydration refers in its preparation method: ultrafilter adopts German Sai Duolisi cross-flow ultrafiltration machine, the filter membrane aperture is 0.2 μ m, and the ultrafiltration concentration standard is for per hour concentrating the following liquid 10kg of molecular weight 30,000 dalton.
4. a kind of organic bovine colostrum as claimed in claim 1 or 2, it is characterized in that preparing successively by the following method: raw material is on the waiting list; High speed centrifugation degreasing impurity elimination; The ultrafiltration concentration dehydration; Dry powder process; Ultramicro grinding; Gamma-radiation irradiation sterilization and getting.
5. a kind of organic bovine colostrum as claimed in claim 4, it is characterized in that by the following method preparation before ultrafiltration concentration dehydration: choose endocrine healthy colostrum on the 3rd from the healthy cow farrowing, and at once be stored under the cryogenic conditions colostrum of collecting for subsequent use; The colostrum raw material of collecting is carried out the centrifugal degreasing impurity elimination in high speed low temperature centrifugal machine;
Wherein, between the colostrum pH value 6.2～6.5, relative density is that solid content is more than 12% between 1.030 ~ 1.060; Cryogenic conditions refers to that temperature is 0-4 ℃; Parameter of noncentricity is 6000-7000r/min, centrifugation time 15-20min.
6. such as claim 4 or 5 described a kind of organic bovine colostrums, it is characterized in that by the following method preparation before the gamma-radiation irradiation sterilization after the ultrafiltration concentration dehydration: the concentrated colostrum that ultrafiltration is good places special-purpose tray, pallet is put into air dry oven carry out air-dry; The wafer that drying is good carries out ultramicro grinding with micronizer; Wherein, the drying box temperature setting is set to 50-55 ℃, time 36-48h; Colostrum powder granular size after the ultramicro grinding is more than 80 orders.
7. the preparation method of an organic bovine colostrum is characterized in that preparing the dehydration of employing ultrafiltration concentration and gamma-radiation irradiation sterilization in the link at bovine colostrum; Wherein, the ultrafiltration concentration dehydration refers to: ultrafilter filter membrane aperture is 0.1-0.3 μ m, and the ultrafiltration standard is for per hour concentrating the following liquid 8-12kg of molecular weight 30,000 dalton; The gamma-radiation irradiation sterilization refers to: dose of radiation is 6-8kGy.
8. the preparation method of a kind of organic bovine colostrum as claimed in claim 7, it is characterized in that preparing successively by the following method: raw material is on the waiting list; High speed centrifugation degreasing impurity elimination; The ultrafiltration concentration dehydration; Dry powder process; Ultramicro grinding; Gamma-radiation irradiation sterilization and getting;
Wherein, by the following method preparation before ultrafiltration concentration dehydration: choose endocrine healthy colostrum on the 3rd from the healthy cow farrowing, and at once be stored under the cryogenic conditions colostrum of collecting for subsequent use; The colostrum raw material of collecting is carried out the centrifugal degreasing impurity elimination in high speed low temperature centrifugal machine; Between the colostrum pH value 6.2～6.5, relative density is that solid content is more than 12% between 1.030 ~ 1.060; Cryogenic conditions refers to that temperature is 0-4 ℃; Parameter of noncentricity is 6000-7000r/min, centrifugation time 15-20min;
By the following method preparation before the gamma-radiation irradiation sterilization after the ultrafiltration concentration dehydration: the concentrated colostrum that ultrafiltration is good places special-purpose tray, pallet is put into air dry oven carry out air-dry; The wafer that drying is good carries out ultramicro grinding with micronizer; The drying box temperature setting is set to 50-55 ℃, time 36-48h; Colostrum powder granular size after the ultramicro grinding is more than 80 orders.
9. such as claim 1,2 or 5 described a kind of organic bovine colostrums, it is characterized in that according to conventional method, make mixture, concentrated pill, capsule, pill, granule, tablet, pill, soft capsule, sustained release agent, oral liquid or the freeze drying powder injection of clinical acceptance.
10. such as claim 1, the application of 2 or 5 described a kind of organic bovine colostrums in preparation colostrum health products.
11. such as claim 1,2 or 5 described a kind of organic bovine colostrums, it is characterized in that the preparation method of its capsule is: with capsule filling machine can colostrum powder, capsule standard is 0.5g/ grain ± 10mg; Capsule after the can is made capsule board via flat Autoblisterpackagingmachine after the polishing machine polishing, check that screening indefectible rear mounted box makes finished product.
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| CN103891900A (en) * | 2014-04-15 | 2014-07-02 | 南京中科集团股份有限公司 | Processing method of bovine colostrum |
| CN108308280A (en) * | 2018-03-07 | 2018-07-24 | 西北师范大学 | A method of preparing donkey milk freeze-dried powder under cryogenic |
| CN109198029A (en) * | 2018-09-27 | 2019-01-15 | 芜湖市好亦快食品有限公司三山分公司 | Sea-buckthorn bovine colostrum and its production method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103891900A (en) * | 2014-04-15 | 2014-07-02 | 南京中科集团股份有限公司 | Processing method of bovine colostrum |
| CN108308280A (en) * | 2018-03-07 | 2018-07-24 | 西北师范大学 | A method of preparing donkey milk freeze-dried powder under cryogenic |
| CN109198029A (en) * | 2018-09-27 | 2019-01-15 | 芜湖市好亦快食品有限公司三山分公司 | Sea-buckthorn bovine colostrum and its production method |
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