CN103045461A - Method for continuously producing biological cellulose gel - Google Patents
Method for continuously producing biological cellulose gel Download PDFInfo
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- CN103045461A CN103045461A CN2013100046354A CN201310004635A CN103045461A CN 103045461 A CN103045461 A CN 103045461A CN 2013100046354 A CN2013100046354 A CN 2013100046354A CN 201310004635 A CN201310004635 A CN 201310004635A CN 103045461 A CN103045461 A CN 103045461A
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Abstract
The invention relates to equipment for continuously producing biological cellulose gel, and a method for producing the biological cellulose gel with the equipment. The equipment comprises a tank body, a culture medium inlet formed at the upper part of the tank body, a culture medium outlet formed at the bottom of the tank body, air holes, a central shaft, and at least a group of paddles connected with the central shaft, wherein each group of paddles at least comprises a blade; the central shaft can rotationally drive the paddles 6 to rotate in the tank body; the central shaft connected with the paddles can be dismounted from the tank body and then mounted again; the central shaft and the paddles are hollow; a plurality of breathing holes are formed in the external surfaces of the paddles; and an air inlet is formed in the central shaft. With the adoption of the equipment and the method, continuous fermentation production of the biological cellulose gel is achieved, the fermentation efficiency is improved effectively, the fermentation time is shortened, harvest can be achieved rapidly, and the production efficiency is improved greatly.
Description
Technical field
The present invention relates to production unit and the method for biology cellulose gel, be specifically related to a kind of equipment of continuous production biology cellulose gel and with the method for this device fabrication biology cellulose gel.
Background technology
Biology cellulose (Biocellulose) is called again bacteria cellulose (Bacterial cellulose), and the product of drying and dehydrating is not gel, therefore is called as the biology cellulose gel.It is normally by the synthetic Mierocrystalline cellulose of the bacteriums such as acetic acid Pseudomonas.It has identical molecular structure unit with the natural cellulose that plant or marine alga produce, but does not wherein have the association products such as xylogen, pectin and hemicellulose, has higher degree of crystallinity and the polymerization degree of Geng Gao.Biology cellulose also has superfine space reticulated structure, and the fento by diameter 3-4 nanometer forms Mierocrystalline cellulose usually, and is intertwined to form flourishing space reticulated structure.These characteristics are so that biology cellulose has the water-holding power of better physical strength and superelevation, and have good biocompatibility, adaptability and biodegradability.At present in field widespread uses such as food, medicine, papermaking, weavings.
The production of biology cellulose, normal static cultivation or the dynamic cultivation of using, bacterial classification acetobacter xylinum commonly used or gluconate pyracetobacillus, substratum is commonly used natural medium, such as Sucus Cocois substratum, pineapple juice substratum, corn steep liquor substratum etc., human configuration substratum (substratum and the mixed culture medium that namely directly form with the configuration of the compositions such as glucose, peptone, i.e. interpolation nutritive ingredient and the substratum that makes in the natural component).Static cultivation is called again the tray cultivation or cultivate on the surface, the liquid substratum of namely in tray, packing into, and the cultivation of inoculation strain fermentation can generate the biology cellulose gel product on the liquid medium within liquid level after 8-15 days.The biology cellulose product space reticulated structure that this method obtains is comparatively fine and close, and the quality of product is higher, for example has good mechanical property, and mouthfeel is sharp and clear etc.Dynamic cultivation still rests on the laboratory study stage at present, it is to adopt the mode of shaking table or stir culture to produce fibres element gel next life, although the efficient of this method fermentation is higher, the space reticulated structure that obtains product is comparatively loose, and mechanical property is relative with mouthfeel relatively poor.No matter be above-mentioned static cultivation method or dynamic cultivation method, all can't realize serialization production, operational difficulty; And static cultivation method fermentation efficiency is low, and this is because in the static fermentation process, the biology cellulose gel meeting starvation (air) that progressively produces causes follow-up fermentation efficiency to reduce; Although and dynamic cultivation can guarantee higher fermentation efficiency, the quality of its product but has been subject to considerable influence, can't promote the use of.
Summary of the invention
For defects, the present invention at first provides a kind of equipment of continuous production biology cellulose gel, it comprises: tank body, be arranged on the substratum import on tank body top and be arranged on substratum outlet, pore, the central shaft of tank base and be connected at least one group of blade on the central shaft, wherein every group of blade comprises a blade at least, and the rotation of central shaft can drive blade and rotate in tank body.
In the above-mentioned equipment, tank body is the interlayer tank body preferably, and is provided with import and the outlet of insulation solution.
In the above-mentioned equipment, the preferred center axle is externally connected with CD-ROM drive motor at tank body, drives the central shaft rotation by CD-ROM drive motor.
In the above-mentioned equipment, preferred center axle and blade all are hollow, and are provided with a plurality of breather holes on the outside surface of blade, also are provided with inlet mouth on the central shaft.
In the above-mentioned equipment, the preferred center axle is made with stainless steel.
In the above-mentioned equipment, preferred blade is made with polyethylene.
In the above-mentioned equipment, the outside surface of preferred blade is the uneven surface through polishing.
In the above-mentioned equipment, but preferably be provided with the tank body hatch door of opening and closing of fault in a side of tank body, the size of this tank body hatch door is greater than the size of every group of blade.
In the above-mentioned equipment, the central shaft that preferably is connected with blade can and be reinstalled from the tank body dismounting.
In the above-mentioned equipment, preferably being connected on the central shaft every group or every blade can unload and reinstall on the central shaft from central shaft.
The present invention also provides a kind of usefulness method that above-mentioned equipment is produced bacterial cellulose gel continuously, specifically comprises:
1) sealing substratum outlet keeps pore unobstructed, injects at least 70 ℃ hot water by the substratum import, keeps after 10-20 minute, opens the substratum outlet, emits hot water, equipment is cleaned and sterilize;
2) seal again the substratum outlet, and keep pore unobstructed, contact with blade at least by liquid nutrient medium to the liquid level of having inoculated biology cellulose generation bacterium after the substratum import injection sterilization, start CD-ROM drive motor, begin fermentation;
3) open the tank body hatch door, the central shaft that is connected with blade is unloaded from tank body, results biology cellulose gel.
In the step 1) of aforesaid method, preferably after injecting hot water, open CD-ROM drive motor, drive central shaft and on the blade rotation; Close CD-ROM drive motor before opening the substratum outlet.
The step 2 of aforesaid method) in, through the injection rate of the liquid nutrient medium of inoculation preferably liquid level be no more than the height of central shaft.
The step 2 of aforesaid method) in, preferably the import by insulation solution and outlet are at the thermal insulation medium of 25-32 ℃ of the interlayer internal recycle of tank body.
The step 2 of aforesaid method) in, the rotating speed 20-120rpm of CD-ROM drive motor 7.
The step 2 of aforesaid method) in.Fermentation time, the substratum import is also sealed, by pore delivering oxygen in the tank body.
In the step 3) of aforesaid method, preferably blow to the hollow interior of central shaft and blade by the inlet mouth on the central shaft that unloads with air pump, so that the biology cellulose gel can promptly separate from blade, thereby gather in the crops fast the biology cellulose gel.
In the aforesaid method, after for some time of preferably fermenting by substratum import supplementary carbon source, nitrogenous source and/or sterilized water in the substratum.
In the aforesaid method, after a preferred batch fermentation is finished, replace tank body interior central shaft and blade with central shaft sterilization, that be connected with blade, proceed fermentation.
The equipment that provides and method among the present invention, the continuous nutritive substance in the supplemental medium, and realize the production of continuously fermenting of biology cellulose gel by the mode of replacing central shaft and blade; And the rotation by blade, make its adsorptive liquid substratum when contacting with liquid nutrient medium, when leaving the liquid nutrient medium liquid level, it can obtain sufficient oxygen supply, even formed the biology cellulose gel at blade, because liquid nutrient medium still can be adsorbed on the biology cellulose gel that has generated, still can obtain sufficient oxygen after it leaves the liquid nutrient medium liquid level, therefore the efficient of energy Effective Raise fermentation shortens fermentation time.Adopt air blast fast the biology cellulose gel to be separated with blade with central shaft, realize fast results.
Description of drawings
Accompanying drawing 1 is the positive cross-sectional schematic of equipment among the present invention;
Accompanying drawing 2 is side cross-sectional schematic of equipment among the present invention.
Embodiment
Following examples only are used for explanation the present invention, and it should not limit the scope of this invention, and any change that can be easy to make in this area can be deemed to be within the scope of the present invention.
Embodiment 1 usefulness equipment of the present invention and method are produced the biology cellulose gel continuously
Sealing substratum outlet 3 keeps pore 4 unobstructed, injects 70 ℃ hot water by substratum import 2, open CD-ROM drive motor 7, drive central shaft 5 and on blade 6 rotations, be provided with a plurality of breather holes 8 on the outside surface of blade 6, also be provided with inlet mouth on the central shaft; Keep after 20 minutes, close and open substratum outlet 3 behind the CD-ROM drive motor and emit hot water, finish equipment is cleaned and sterilizes; And then sealing substratum outlet 3, and keep pore 4 unobstructed, contact with blade at least by liquid nutrient medium to the liquid level of having inoculated biology cellulose generation bacterium after the substratum import 2 injection sterilizations, but be no more than central shaft 5, start CD-ROM drive motor 7, adjusting rotary speed is that 20rpm begins fermentation; After 5 days, open tank body hatch door 10, the central shaft 5 that is connected with blade 6 is unloaded from tank body, reinstall central shaft sterilization, that be connected with blade, proceed fermentation; Blow to the hollow interior of central shaft 5 and blade 6 by the inlet mouth 9 on the central shaft of replacing with air pump, the biology cellulose gel is separated with blade 6 with central shaft 5, results biology cellulose gel.
Sealing substratum outlet 3, keep pore 4 unobstructed, inject 90 ℃ hot water by substratum import 2, open CD-ROM drive motor 7, drive central shaft 5 and on blade 6 rotations, keep after 10 minutes, close and open substratum outlet 3 behind the CD-ROM drive motor and emit hot water, finish equipment is cleaned and sterilizes; And then sealing substratum outlet 3, and keep pore 4 unobstructed, at least contact with blade by liquid nutrient medium to the liquid level of having inoculated biology cellulose generation bacterium after the substratum import 2 injection sterilizations, but be no more than central shaft 5, start CD-ROM drive motor 7, adjusting rotary speed is 120rpm, closes substratum import 2, begins fermentation by pore to tank body 1 interior delivering oxygen; After 8 days, open tank body hatch door 10, the central shaft 5 that is connected with blade 6 is unloaded from tank body, reinstall central shaft sterilization, that be connected with blade, proceed fermentation; Blow to the hollow interior of central shaft 5 and blade 6 by the inlet mouth 9 on the central shaft of replacing with air pump, the biology cellulose gel is separated with blade 6 with central shaft 5, results biology cellulose gel.
The above only is preferred implementation of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the technology of the present invention principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (18)
1. equipment of producing continuously the biology cellulose gel, it is characterized in that: this equipment comprises tank body (1), be arranged on the substratum import (2) on tank body (1) top and be arranged on substratum outlet (3), pore (4), the central shaft (5) of tank base and be connected at least one group of blade (6) on the central shaft (5), wherein every group of blade comprises a blade at least, and central shaft (5) can rotarily drive blade (6) and rotate in tank body.
2. equipment according to claim 1, it is characterized in that: tank body (1) is the interlayer tank body, and is provided with import and the outlet of thermal insulation medium.
3. equipment according to claim 1 and 2, it is characterized in that: central shaft (5) is externally connected with CD-ROM drive motor (7) at tank body (1), drives the central shaft rotation by CD-ROM drive motor (7).
4. equipment according to claim 1, it is characterized in that: central shaft (5) and blade (6) all are hollow, and are provided with a plurality of breather holes (8) on the outside surface of blade (6), also are provided with inlet mouth (9) on the central shaft (5).
5. according to claim 1 or 4 described equipment, it is characterized in that: central shaft (5) is made with stainless steel.
6. equipment according to claim 5, it is characterized in that: blade (6) is made with polyethylene.
7. equipment according to claim 6 is characterized in that: the outside surface of blade (6) is the uneven surface through polishing.
8. equipment according to claim 1 is characterized in that: at tank body (1) but a side be provided with the tank body hatch door (10) of opening and closing of fault, the size of this tank body hatch door (10) is greater than the size of one group of blade (6).
9. according to claim 1 or 4 described equipment, it is characterized in that: the central shaft that is connected with blade (6) can be dismantled and reinstall from tank body (1).
10. method of producing continuously bacterial cellulose gel with one of the described equipment of claim 1-6, it is characterized in that: step comprises:
1) sealing substratum outlet (3) keeps pore (4) unobstructed, injects at least 70 ℃ hot water by substratum import (2), keeps after 10-20 minute, opens substratum outlet (3), emits hot water;
2) seal again substratum outlet (3), and keep pore (4) unobstructed, contact with blade at least by liquid nutrient medium to the liquid level of having inoculated biology cellulose generation bacterium after substratum import (2) the injection sterilization, start CD-ROM drive motor (7), begin fermentation;
3) open tank body hatch door (10), the central shaft (5) that will be connected with blade (6) unloads from tank body, results biology cellulose gel.
11. method according to claim 7 is characterized in that: in the step 1), after injecting hot water, open CD-ROM drive motor (7), drive central shaft (5) and on blade (6) rotation; Open substratum outlet (3) and close before CD-ROM drive motor (7).
12. method according to claim 7 is characterized in that: step 2) in, the injection rate of the liquid nutrient medium of process inoculation is the height that liquid level is no more than central shaft (5).
13. method according to claim 7 is characterized in that: step 2) in, by thermal insulation medium import and the outlet thermal insulation medium 25-32 ℃ of the interlayer internal recycle of tank body (1).
14. according to claim 7 or 8 described methods, it is characterized in that: step 2) in, the rotating speed 20-120rpm of CD-ROM drive motor (7).
15. method according to claim 7 is characterized in that: step 2) in, sealing substratum import (2) in fermentation, and pass through pore (4) to the interior delivering oxygen of tank body (1).
16. method according to claim 7, it is characterized in that: in the step 3), blow to the hollow interior of central shaft (5) and blade (6) by the inlet mouth (9) on the central shaft (5) that unloads with air pump, the biology cellulose gel is separated with blade (6) with central shaft (5).
17. method according to claim 7 is characterized in that: after fermentation for some time by substratum import (2) supplementary carbon source, nitrogenous source and/or sterilized water in the tank body.
18. method according to claim 7 is characterized in that: after a batch fermentation is finished, replace tank body interior former central shaft and blade with central shaft sterilization, that be connected with blade, ferment continuously.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310004635.4A CN103045461B (en) | 2013-01-07 | 2013-01-07 | Method for continuously producing biological cellulose gel |
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| CN201310004635.4A CN103045461B (en) | 2013-01-07 | 2013-01-07 | Method for continuously producing biological cellulose gel |
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| CN103045461B CN103045461B (en) | 2014-07-16 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484356A (en) * | 2013-09-18 | 2014-01-01 | 天津大学 | Fermentation reactor and preparation method for dynamically culturing and preparing ordered bacteria cellulose |
| CN107287257A (en) * | 2016-03-31 | 2017-10-24 | 海南椰国食品有限公司 | By circulating fed-batch fermentation while producing the method for biology cellulose and rice vinegar |
| CN108239597A (en) * | 2018-04-03 | 2018-07-03 | 泉州市南方食品机械有限公司 | A kind of stirrer paddle of coconut palm fruit fermentation tank |
| CN109963705A (en) * | 2016-08-26 | 2019-07-02 | 奥特发德国科技有限公司 | Feeding device, baling press and means of defence |
| CN115176647A (en) * | 2022-06-14 | 2022-10-14 | 华南农业大学 | Method for producing edible fungus membrane by using fed batch liquid culture medium static fermentation method, fungus membrane and application |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103484356A (en) * | 2013-09-18 | 2014-01-01 | 天津大学 | Fermentation reactor and preparation method for dynamically culturing and preparing ordered bacteria cellulose |
| CN103484356B (en) * | 2013-09-18 | 2015-03-04 | 天津大学 | Fermentation reactor and preparation method for dynamically culturing and preparing ordered bacteria cellulose |
| CN107287257A (en) * | 2016-03-31 | 2017-10-24 | 海南椰国食品有限公司 | By circulating fed-batch fermentation while producing the method for biology cellulose and rice vinegar |
| CN109963705A (en) * | 2016-08-26 | 2019-07-02 | 奥特发德国科技有限公司 | Feeding device, baling press and means of defence |
| CN109963705B (en) * | 2016-08-26 | 2021-06-11 | 奥特发德国科技有限公司 | Feeding device, packaging machine and protection method |
| US11465380B2 (en) | 2016-08-26 | 2022-10-11 | Autefa Solutions Germany Gmbh | Feed device, baling press, and protection process |
| CN108239597A (en) * | 2018-04-03 | 2018-07-03 | 泉州市南方食品机械有限公司 | A kind of stirrer paddle of coconut palm fruit fermentation tank |
| CN115176647A (en) * | 2022-06-14 | 2022-10-14 | 华南农业大学 | Method for producing edible fungus membrane by using fed batch liquid culture medium static fermentation method, fungus membrane and application |
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| CN103045461B (en) | 2014-07-16 |
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