CN103041439A - Carbon nanotube-cell growth factor composite dressing and preparation method thereof - Google Patents
Carbon nanotube-cell growth factor composite dressing and preparation method thereof Download PDFInfo
- Publication number
- CN103041439A CN103041439A CN201210330471XA CN201210330471A CN103041439A CN 103041439 A CN103041439 A CN 103041439A CN 201210330471X A CN201210330471X A CN 201210330471XA CN 201210330471 A CN201210330471 A CN 201210330471A CN 103041439 A CN103041439 A CN 103041439A
- Authority
- CN
- China
- Prior art keywords
- growth factor
- cell growth
- cnt
- carbon nanotube
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a preparation method of a carbon nanotube-cell growth factor composite dressing. The method comprises the following steps that: (1) acidification of a carbon nanotube; (2) preparation of a carbon nanotube-polyvinyl alcohol-cell growth factor composite solution; and (3) electrostatic spinning. The carbon nanotube-polyvinyl alcohol composite a nonwoven material with uniform texture, a few pearls, straight and continuous fibers and uniform diameter can be obtained, a specific surface area is larger, and pores in a layer ensure the air circulation to prevent liquids such as water from entering, thus effectively protecting wounds and preventing infection, but also ensuring the ventilation of wounds; simultaneously, the preparation method disclosed by the invention can well compound a cell growth factor to serve as a drug carrier to form a sustained-release controlled release system, thereby promoting wound healing and facilitating wound recovery as soon as possible.
Description
Technical field
The present invention relates to the preparation method of a kind of CNT-cell growth factor combine dressing.
Background technology
Skin plays a part the protection organismic internal environment and keeps that it is stable, and the final goal that skin burns, treat after the wound is exactly wound healing, rebuilds or recovers skin barrier function.Along with to the going deep into of researching wound healing mechanism, people recognize that it is for flap coverage that the purpose of using dressing runs far deeper than, and also should have the multi-efficiency of absorption secretions, kill bacteria and promotion wound healing.Nanometer dressing has very strong adsorptivity, can play the antibacterial film barrier action, prevents traumatic infection, hemorrhage, promotes wound repair and becomes in recent years study hotspot.CNT dressing is the nanometer adjuvant of present comparative maturity, have the curative effect of certain antibiotic and wound healing, and toxic and side effects is little, and is easy to use.But it is relatively poor that the shortcoming of CNT dressing is water absorption, and satellite is not good enough, strong to the wound surface adhesion, and giving changes dressings brings certain difficulty, and therefore wound healing awaits further Improvement well.
Summary of the invention
The present invention aims to provide the preparation method of a kind of CNT-cell growth factor combine dressing.
In order to achieve the above object, the present invention includes following steps:
1. acidify CNT:
(1) concentrated sulphuric acid 20～100ml acidify CNT: with the 85%-98.3%(percentage by weight), 65%-85%(percentage by weight) concentrated nitric acid 10～50 ml and particle diameter be to put into again 60 ℃～100 ℃ water-bath acidify 0.5 h～2 h after 30 nm～500 nm CNT 0.1g～2.0g are uniformly dispersed;
(2) filtering and washing: be that the sucking filtration film of 0.11 μ m-0., 33 μ m carries out sucking filtration with the aperture, and be washed with distilled water to cleaning mixture pH value 5.0～8.0;
(3) drying is weighed: the CNT that will leach through step (2) is 40 ℃ of-70 ℃ of oven dry;
(4) dissolve centrifugal calculating: the CNT that (3) are obtained is dissolved in the 50 ml distilled water, room temperature ultra-sonic dispersion 0.5 h-3h, and again at the centrifugal 5mins of 8000rpm-20000rpm～30 mins, then precipitation is dry;
2. prepare carbon nanotube-polyvinyl alcohol-cell growth factor composite solution:
(1) CNT being added deionized water is uniformly dispersed.
(2) take the gained carbon nano tube dispersion liquid as liquid medium, add in the polyvinyl alcohol.At 60 ℃～100 ℃ lower heating in water bath, stir;
(3) add cell growth factor powder or cell growth factor solution to the solution the inside through the above-mentioned steps gained, making the cell growth factor final concentration is 0.01 μ g/ml～1000 μ g/ml, the vibration of room temperature magnetic force or ultra-sonic dispersion 0.5 h～8 h.Biological electron microscope characterizes and shows that it is uniformly dispersed it again.
3. static weaving:
Carbon nanotube-polyvinyl alcohol-cell growth factor composite solution is carried out electrostatic spinning, setting voltage scope 20kv～70kv(10-200).
Adopt preparation method of the present invention can obtain even tissue; send out pearl less; fiber is straight; continuously; the carbon nanotube-polyvinyl alcohol of diameter homogeneous-cell growth factor compound nonwoven cloth material; a kind of porous materials that nanometer micropore is arranged by these fibroplastic non-woven fabrics; therefore larger specific surface area is arranged; and layer mesopore guaranteed the circulation of air; can intercept going deep into of the liquid such as water; so not only can effectively protect wound; protect from infection; and guaranteed the ventilation of wound; adopt simultaneously well compound cells somatomedin and form the sustained-release and controlled release system as pharmaceutical carrier of preparation method of the present invention; promote the healing of wound, be conducive to wound and restore as early as possible.And guaranteed well the activity of cell growth factor
The specific embodiment
Embodiment 1:
1. acidify CNT:
(1) acidify CNT: be 30 nm～500 nm CNT 0.1g～2.0g ultra-sonic dispersion 0.5 h-3h with concentrated nitric acid 20 ml and the particle diameter of concentrated sulphuric acid 60ml, the 65%-85% of 85%-98.3%, put into again 60 ℃～100 ℃ water-bath acidify 0.5 h～2 h;
(2) filtering and washing: be that the sucking filtration film of 0.11 μ m-0., 33 μ m carries out sucking filtration with the aperture, and be washed with distilled water to cleaning mixture pH value 5.0～8.0;
(3) drying is weighed: the CNT that will leach through step (2) is 40 ℃ of-70 ℃ of oven dry;
(4) dissolve centrifugal calculating: the CNT that (3) are obtained is dissolved in the 50 ml distilled water, room temperature ultra-sonic dispersion 0.5 h-3h, and again at the centrifugal 5mins of 8000rpm-20000rpm～30 mins, then precipitation is dry;
2. prepare carbon nanotube-polyvinyl alcohol-cell growth factor composite solution:
(1) the CNT 10mg that acidify is obtained puts into beaker, add deionized water 1000ml, 5 W～100 W magnetic agitation, 0.1 h～6 h, 10khz-40 khz frequency ultra-sonic dispersion 0.5 h～3 h again, obtain homogeneous, stable, without the dispersion soln of precipitation.(2) take gained CNT aqueous dispersions as liquid medium, adding the 0.01ml-10ml(mass fraction is 1%-50%) polyvinyl alcohol.At 60 ℃～100 ℃ lower heating in water bath 1 h～3 h.Continue to stir, take guarantee final gained as homogeneous, stable, the dispersion liquid that precipitates or flocculate do not appear.
(3) add cell growth factor powder or cell growth factor solution to the solution the inside through the above-mentioned steps gained, making the cell growth factor final concentration is 0.01 μ g/ml～1000 μ g/ml, and the vibration of room temperature magnetic force or ultra-sonic dispersion 0.5 h～8 h are until be uniformly dispersed.
3. static weaving:
Carbon nanotube-polyvinyl alcohol-cell growth factor composite solution is poured in the syringe, and syringe is fixed on the syringe pump.The opening high pressure generator, the beginning electrostatic spinning is collected the gained sample at trapping medium (metal forming).Setting voltage scope 20kv～70kv carries out the static weaving, obtains non-woven fabrics.
Adopt preparation method of the present invention can obtain even tissue; send out pearl less; fiber is straight; continuously; the carbon nanotube-polyvinyl alcohol compound nonwoven cloth material of diameter homogeneous; a kind of porous materials that nanometer micropore is arranged by these fibroplastic non-woven fabrics; therefore larger specific surface area is arranged; and layer mesopore guaranteed the circulation of air; can intercept going deep into of the liquid such as water; so not only can effectively protect wound; protect from infection; and guaranteed the ventilation of wound; adopt simultaneously well compound cells somatomedin and form the sustained-release and controlled release system as pharmaceutical carrier of preparation method of the present invention; promote the healing of wound, be conducive to wound and restore as early as possible.
Embodiment 2:
1. acidify CNT:
(1) acidify CNT: be 30 nm～500 nm CNT 0.1g～2.0g ultra-sonic dispersion 0.5 h-3h with concentrated nitric acid 20 ml and the particle diameter of concentrated sulphuric acid 60ml, the 65%-85% of 85%-98.3%, put into again 60 ℃～100 ℃ water-bath acidify 0.5 h～2 h;
(2) filtering and washing: be that the sucking filtration film of 0.11 μ m-0., 33 μ m carries out sucking filtration with the aperture, and be washed with distilled water to cleaning mixture pH value 5.0～8.0;
(3) drying is weighed: the CNT that will leach through step (2) is 40 ℃ of-70 ℃ of oven dry;
(4) dissolve centrifugal calculating: the CNT that (3) are obtained is dissolved in the 50 ml distilled water, room temperature ultra-sonic dispersion 0.5 h-3h, and again at the centrifugal 5mins of 8000rpm-20000rpm～30 mins, then precipitation is dry;
2. prepare carbon nanotube-polyvinyl alcohol-cell growth factor composite solution:
(1) the CNT 10mg that acidify is obtained puts into beaker, add deionized water 1000ml, 5 W～100 W magnetic agitation, 0.1 h～6 h, 10khz-40 khz frequency ultra-sonic dispersion 0.5 h～3 h again, obtain homogeneous, stable, without the dispersion soln of precipitation.(2) take gained CNT aqueous dispersions as liquid medium, adding the 0.01ml-10ml(mass fraction is 1%-50%) polyvinyl alcohol.At 60 ℃～100 ℃ lower heating in water bath 1 h～3 h.Continue to stir, take guarantee final gained as homogeneous, stable, the dispersion liquid that precipitates or flocculate do not appear.
(3) add cell growth factor powder or cell growth factor solution to the solution the inside through the above-mentioned steps gained, making the cell growth factor final concentration is 0.01 μ g/ml～1000 μ g/ml, and the vibration of room temperature magnetic force or ultra-sonic dispersion 0.5 h～8 h are until be uniformly dispersed.
3. static weaving:
Carbon nanotube-polyvinyl alcohol-cell growth factor composite solution is carried out electrostatic spinning, setting voltage scope 20kv～70kv.
Adopt preparation method of the present invention can obtain even tissue; send out pearl less; fiber is straight; continuously; the carbon nanotube-polyvinyl alcohol compound nonwoven cloth material of diameter homogeneous; a kind of porous materials that nanometer micropore is arranged by these fibroplastic non-woven fabrics; therefore larger specific surface area is arranged; and layer mesopore guaranteed the circulation of air; can intercept going deep into of the liquid such as water; so not only can effectively protect wound; protect from infection; and guaranteed the ventilation of wound; adopt simultaneously well compound cells somatomedin and form the sustained-release and controlled release system as pharmaceutical carrier of preparation method of the present invention; promote the healing of wound, be conducive to wound and restore as early as possible.
Embodiment 3:
1. acidify CNT:
(1) acidify CNT: be 30 nm～500 nm CNT 0.1g～2.0g ultra-sonic dispersion 0.5 h-3h with concentrated nitric acid 20 ml and the particle diameter of concentrated sulphuric acid 60ml, the 65%-85% of 85%-98.3%, put into again 60 ℃～100 ℃ water-bath acidify 0.5 h～2 h;
(2) filtering and washing: be that the sucking filtration film of 0.11 μ m-0., 33 μ m carries out sucking filtration with the aperture, and be washed with distilled water to cleaning mixture pH value 5.0～8.0;
(3) drying is weighed: the CNT that will leach through step (2) is 40 ℃ of-70 ℃ of oven dry;
(4) dissolve centrifugal calculating: the CNT that (3) are obtained is dissolved in the 50 ml distilled water, room temperature ultra-sonic dispersion 0.5 h-3h, and again at the centrifugal 5mins of 8000rpm-20000rpm～30 mins, then precipitation is dry;
2. prepare carbon nanotube-polyvinyl alcohol-cell growth factor composite solution:
(1) the CNT 10mg that acidify is obtained puts into beaker, add deionized water 1000ml, 5 W～100 W magnetic agitation, 0.1 h～6 h, 10khz-40 khz frequency ultra-sonic dispersion 0.5 h～3 h again, obtain homogeneous, stable, without the dispersion soln of precipitation.
(2) take gained CNT aqueous dispersions as liquid medium, adding the 0.01ml-10ml(mass fraction is 1%-50%) polyvinyl alcohol.At 60 ℃～100 ℃ lower heating in water bath 1 h～3 h.Continue to stir, take guarantee final gained as homogeneous, stable, the dispersion liquid that precipitates or flocculate do not appear.
(3) add cell growth factor powder or cell growth factor solution to the solution the inside through the above-mentioned steps gained, making the cell growth factor final concentration is 0.01 μ g/ml～1000 μ g/ml, and the vibration of room temperature magnetic force or ultra-sonic dispersion 0.5 h～8 h are until be uniformly dispersed.
3. static weaving:
Carbon nanotube-polyvinyl alcohol-cell growth factor composite solution is carried out electrostatic spinning, setting voltage scope 20kv～70kv.
Adopt preparation method of the present invention can obtain even tissue; send out pearl less; fiber is straight; continuously; the carbon nanotube-polyvinyl alcohol compound nonwoven cloth material of diameter homogeneous; a kind of porous materials that nanometer micropore is arranged by these fibroplastic non-woven fabrics; therefore larger specific surface area is arranged; and layer mesopore guaranteed the circulation of air; can intercept going deep into of the liquid such as water; so not only can effectively protect wound; protect from infection; and guaranteed the ventilation of wound; adopt simultaneously well compound cells somatomedin and form the sustained-release and controlled release system as pharmaceutical carrier of preparation method of the present invention; promote the healing of wound, be conducive to wound and restore as early as possible.
Claims (2)
1. the preparation method of CNT-cell growth factor combine dressing is characterized in that, may further comprise the steps:
(1) acidify CNT:
(1) concentrated nitric acid 10～50ml and the particle diameter with the concentrated sulphuric acid 20～100ml, 65～85% (percentage by weight) of 85～98.3% (percentage by weights) is to put into 60～100 ℃ of water-bath acidify 0.5～2h after 5～500nm CNT, 0.1～2.0g is uniformly dispersed again;
(2) be that the sucking filtration film of 0.11～0.33 μ m carries out sucking filtration with the aperture, and be washed with distilled water to cleaning mixture pH value 5.0～8.0;
The CNT that (3) will leach through step (2) is 40～70 ℃ of oven dry;
(4) CNT that (3) is obtained is dissolved in the distilled water, is uniformly dispersed, and precipitation is dry;
(2) preparation carbon nanotube-polyvinyl alcohol-cell growth factor composite solution:
(1) CNT being added deionized water is uniformly dispersed;
(2) take the gained carbon nano tube dispersion liquid as liquid medium, add in the polyvinyl alcohol, the mass fraction that makes CNT is 1～50%, at 60～100 ℃ of lower heating in water bath, stirs;
(3) add cell growth factor powder or cell growth factor solution to the solution the inside through the above-mentioned steps gained, making the cell growth factor final concentration is 0.01～1000 μ g/ml, it is uniformly dispersed, makes carbon nanotube-polyvinyl alcohol-cell growth factor composite solution;
(3) static weaving:
Carbon nanotube-polyvinyl alcohol-cell growth factor composite solution is carried out electrostatic spinning, setting voltage scope 10～200kv.
2. according to claim 1 the preparation method of CNT-cell growth factor combine dressing is characterized in that, described electrostatic spinning voltage range is 20～70kv.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210330471XA CN103041439A (en) | 2012-09-07 | 2012-09-07 | Carbon nanotube-cell growth factor composite dressing and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210330471XA CN103041439A (en) | 2012-09-07 | 2012-09-07 | Carbon nanotube-cell growth factor composite dressing and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103041439A true CN103041439A (en) | 2013-04-17 |
Family
ID=48054446
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210330471XA Pending CN103041439A (en) | 2012-09-07 | 2012-09-07 | Carbon nanotube-cell growth factor composite dressing and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103041439A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103741243A (en) * | 2013-12-20 | 2014-04-23 | 北京化工大学常州先进材料研究院 | Preparation method of fiber containing porous covalent organic framework compound |
| CN106994188A (en) * | 2017-03-30 | 2017-08-01 | 中南大学湘雅三医院 | A kind of dressing repaired for radiotherapy |
| CN113398311A (en) * | 2021-05-25 | 2021-09-17 | 周建大 | Polymeric composite protective membrane loaded with nano antibacterial peptide, preparation method and application |
| CN114159613A (en) * | 2021-11-08 | 2022-03-11 | 江苏大学 | Antibacterial and hemostatic composite dressing and preparation method thereof |
| CN114917394A (en) * | 2022-06-24 | 2022-08-19 | 宁夏医科大学 | Double-layer composite functional dressing carrying nano silver and growth factors and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1837274A (en) * | 2005-03-21 | 2006-09-27 | 中国科学院化学研究所 | Degradable and absorbable polymer nano fibrous membrane materials and preparation process and use thereof |
| WO2009108884A2 (en) * | 2008-02-27 | 2009-09-03 | Kci Licensing Inc. | System and method for healing a wound at a tissue site |
| CN102266582A (en) * | 2011-06-17 | 2011-12-07 | 北京化工大学常州先进材料研究院 | Preparation method for drug-loaded nanometer fiber medical dressing |
-
2012
- 2012-09-07 CN CN201210330471XA patent/CN103041439A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1837274A (en) * | 2005-03-21 | 2006-09-27 | 中国科学院化学研究所 | Degradable and absorbable polymer nano fibrous membrane materials and preparation process and use thereof |
| WO2009108884A2 (en) * | 2008-02-27 | 2009-09-03 | Kci Licensing Inc. | System and method for healing a wound at a tissue site |
| CN102266582A (en) * | 2011-06-17 | 2011-12-07 | 北京化工大学常州先进材料研究院 | Preparation method for drug-loaded nanometer fiber medical dressing |
Non-Patent Citations (1)
| Title |
|---|
| 钟石: "碳纳米管-聚乙烯醇-细胞生长因子复合敷料", 《硕士学位论文》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103741243A (en) * | 2013-12-20 | 2014-04-23 | 北京化工大学常州先进材料研究院 | Preparation method of fiber containing porous covalent organic framework compound |
| CN103741243B (en) * | 2013-12-20 | 2016-03-02 | 北京化工大学常州先进材料研究院 | A kind of fiber producing processes containing porous covalent organic framework compound |
| CN106994188A (en) * | 2017-03-30 | 2017-08-01 | 中南大学湘雅三医院 | A kind of dressing repaired for radiotherapy |
| CN113398311A (en) * | 2021-05-25 | 2021-09-17 | 周建大 | Polymeric composite protective membrane loaded with nano antibacterial peptide, preparation method and application |
| CN113398311B (en) * | 2021-05-25 | 2022-08-02 | 周建大 | Polymeric composite protective membrane loaded with nano antibacterial peptide, preparation method and application |
| CN114159613A (en) * | 2021-11-08 | 2022-03-11 | 江苏大学 | Antibacterial and hemostatic composite dressing and preparation method thereof |
| CN114917394A (en) * | 2022-06-24 | 2022-08-19 | 宁夏医科大学 | Double-layer composite functional dressing carrying nano silver and growth factors and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107497182B (en) | Composite nanofiber filtering material with photocatalysis/antibacterial functions and preparation method thereof | |
| Dong et al. | Electrospun nanofibrous materials for wound healing | |
| CN104491914B (en) | A kind of porous pluralgel-nanofiber oxygen flow dressing and preparation method thereof | |
| CN104511045B (en) | A kind of preparation method of the polyvinyl alcohol containing nano silver/chitosan nano fiber membrane dressing | |
| CN103572507A (en) | Preparation method for antibiosis ultraviolet prevention silk fibroin nanofiber membrane | |
| CN103961738B (en) | A kind of chitosan-nanometer silver wound dressing and preparation method thereof | |
| CN103041439A (en) | Carbon nanotube-cell growth factor composite dressing and preparation method thereof | |
| CN103074734B (en) | Preparation method for antibacterial nano-silver composite nanofiber mat by electrostatic spinning | |
| CN102068339B (en) | Preparation method of biodegradable nanofiber medical dressing loaded with medicine | |
| JP2008536022A (en) | Process for producing a layer of polymeric nanofibers by electrospinning from a textile comprising at least one layer of polymeric nanofibers and a polymer solution | |
| Cheng et al. | Environmentally friendly and antimicrobial bilayer structured fabrics with integrated interception and sterilization for personal protective mask | |
| CN104436281A (en) | Preparation method of porous sodium alginate nano-fiber wound dressing | |
| CN101187089A (en) | Silk fibroin and polyvinyl alcohol blending antibacterial nanometer fiber and its preparation method | |
| Li et al. | Daylight-driven rechargeable, antibacterial, filtrating micro/nanofibrous composite membranes with bead-on-string structure for medical protection | |
| CN106048892A (en) | Preparation method of GO/SA/PVA composite nanofiber membrane carrying nano silver particles | |
| CN103320967A (en) | Composite electrostatic spinning material containing fullerene and nano-silver for medical dressings and method for manufacturing composite electrostatic spinning material | |
| Rathinavel et al. | Retracted: Design and fabrication of electrospun SBA-15-incorporated PVA with curcumin: a biomimetic nanoscaffold for skin tissue engineering | |
| CN105664225A (en) | Nano-silver, chitosan and fibroin compound biological dressing and preparation method thereof | |
| CN114086394B (en) | Treatment process of non-woven fabric for mask | |
| CN104288840A (en) | Porous bioglass/chitosan/silk fibroin composite material and preparation method thereof | |
| CN109731121A (en) | A kind of preparation method of the cellulose containing mesoporous silicon oxide and chitosan combine dressing | |
| Chen et al. | Photothermal-responsive fiber dressing with enhanced antibacterial activity and cell manipulation towards promoting wound‐healing | |
| CN104153120A (en) | Antibacterial medical dressing film carrying nano-silver and cellulose nanocrystalline hybrid materials and preparation method thereof | |
| Zhong et al. | Titanium dioxide/quaternary phosphonium salts/polyacrylonitrile composite nanofibrous membranes with high antibacterial properties and ultraviolet resistance efficiency | |
| Liao et al. | Preparation and properties of a novel carbon nanotubes/poly (vinyl alcohol)/epidermal growth factor composite biological dressing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130417 |