CN103014119A - Rapid diagnosis reagent for pathogenic bacillus dysenteriae in medical institution sewage - Google Patents

Abstract

The present invention relates to a rapid diagnosis reagent for pathogenic bacillus dysenteriae in medical institution sewage, wherein the rapid diagnosis reagent is provided for rapidly diagnose pathogenic bacillus dysenteriae in medical institution sewage and excrements. The rapid diagnosis reagent has the following characteristics that: an inoculation amount is small, wherein a small amount of bacteria can rapidly breed, and a result can be observed after culturing for 5-6 h at a room temperature or a temperature of 37 DEG C; false positive results and false negative results are not easily generated; and bacterial inhibition substances existing in samples can be destroyed, such that bacterial growth is not inhibited.

Description

The dysentery bacterium fast diagnosis reagent causes a disease in medical institutions' sewage

Technical field the present invention relates in a kind of medical institutions sewage the dysentery bacterium fast diagnosis reagent that causes a disease, and its main component is that Tryptones, Sodium Citrate, sodium deoxycholate, potassium primary phosphate, dipotassium hydrogen phosphate, sodium-chlor, calf serum, glucose, N.F,USP MANNITOL are made through certain technique.Use for increasing bacterium in medical institutions' sewage and the ight soil and cultivate dysentery bacterium.Utilize the kind of culture experiment discriminating bacteria, it is accurate, quick, easy that its key is.And bacterial load is big or small, can directly affect detected result.Often need that sample is increased bacterium by different methods and cultivate, carry out the discriminating of bacterial species, this is the most frequently used means of present Microbiological Lab.Because at present the range of application of culture condition is limit, the dysentery bacterium in medical institutions' sewage and the ight soil increases fast diagnostic reagent of bacterium, does not have specificly, has that required inoculum size is large, incubation time is grown, be prone to the problems such as false positive or false negative.Along with the severe and standard that country requires medical institutions' sewage and ight soil safety dumping, the research of the dysentery bacterium fast diagnosis reagent that causes a disease in the domestic convection potential mechanism sewage is also in continuous development.Medical personnel, especially Clinical Laboratory person wish to have a kind of above-mentioned deficiency that can overcome always, and cause a disease in medical institutions' sewage the dysentery bacterium fast diagnosis reagent.

Background technology the object of the present invention is to provide a kind of existing differential diagnosis reagent deficiency that can overcome, and cause a disease in medical institutions' sewage the dysentery bacterium fast diagnosis reagent.Diagnostic reagent similar with other compared possesses following characteristics: required inoculum size is little, and a small amount of bacterium can breed rapidly, under common room temperature or 37 ℃ cultivate and got final product observations in 5-6 hour; Be not prone to false positive and false negative; Can destroy the antibacterial substance that exists in the sample, bacterial growth is not suppressed.

Summary of the invention main points of the present invention are to select suitable component, and carry out rational mixture, through heat, dissolve, the techniques such as mixing, cooling, packing, high-temperature sterilization form the dysentery bacterium fast diagnosis reagent that causes a disease in a kind of medical institutions sewage.The prescription following (component and weight percent % thereof) that the present invention selects: Tryptones (1.8-2.2), Sodium Citrate (0.4-0.5), sodium deoxycholate (0.04-0.06), potassium primary phosphate (0.14-0.16), dipotassium hydrogen phosphate (0.38-0.42), sodium-chlor (0.4-0.5), glucose (0.10-0.12), N.F,USP MANNITOL (0.15-0.25), calf serum (0.4-0.5), distilled water add to 100ml.

This diagnostic reagent contains Sodium Citrate and sodium deoxycholate, and Gram-negative bacteria is had restraining effect, intestinal bacteria, and Pseudomonas aeruginosa and Bacillus proteus, poor growth in inoculating 6 hours, and dysentery bacterium can be bred rapidly. sodium-chlor is used for adjusting osmotic pressure; Potassium primary phosphate is used for regulating pH.Tryptones, calf serum, glucose, N.F,USP MANNITOL are the nutrition in the diagnostic reagent and support composition.Be computing time after the specimen inoculation, under common room temperature or 37 ℃ of cultivations, the bacterium of increasing effect arranged all. increase bacterium and cultivate after 6 hours, namely with the method for scoring culture transferring to the enteron aisle selectivity diagnostic reagent to separate pathogenic bacterium.

The preparation method of product of the present invention is:

1), Tryptones, Sodium Citrate, sodium deoxycholate, potassium primary phosphate, dipotassium hydrogen phosphate, sodium-chlor, glucose, N.F,USP MANNITOL are added in the distilled water in proportion, reheats dissolving, correct pH to 7.0 and correct pH to 6.8, use filter paper filtering;

2), pressuresteam sterilization 0.70kg15 minute;

3), under the aseptic condition, add aseptic calf serum, packing ampere bottle, every bottle of about 5ml is stored in the refrigerator for subsequent use.

The present invention has the following advantages: (1) required inoculum size is little, and incubation time is short, and a small amount of bacterium can breed rapidly, under common room temperature or 37 ℃ cultivate and got final product observations in 5-6 hour; (2) be not prone to false positive or false negative; (3) can destroy the bacteriostatic action of the tsiklomitsin, duomycin, terramycin, Liu Suanyan NEOMYCIN SULPHATE, polymyxin and the Streptomycin sulphate that exist in the sample.

The present invention is described in further detail below in conjunction with embodiment for embodiment:

Be configured according to following table institute column data (weight percent %) and described step thereof:

Prescription one:

Prescription two:

Claims (2)

1. the present invention relates to the dysentery bacterium fast diagnosis reagent that causes a disease in a kind of medical institutions sewage, it is characterized in that having following prescription (component and weight percent % thereof): Tryptones 1.8-2.2; Sodium Citrate 0.4-0.5; Sodium deoxycholate 0.04-0.06; Potassium primary phosphate 0.14-0.16; Dipotassium hydrogen phosphate 0.38-0.42; Sodium-chlor 0.4-0.5; Glucose 0.10-0.12; N.F,USP MANNITOL 0.15-0.25; Calf serum 0.4-0.5; Distilled water adds to 100ml.

2. the dysentery bacterium fast diagnosis reagent that causes a disease in the described medical institutions of claim 1 sewage is characterized in that having the method for being prepared as follows:

1), Tryptones, Sodium Citrate, sodium deoxycholate, potassium primary phosphate, dipotassium hydrogen phosphate, sodium-chlor, glucose, N.F,USP MANNITOL are added in the distilled water in proportion, reheats dissolving, correct pH to 7.0 and correct pH to 6.8, use filter paper filtering;

2), pressuresteam sterilization 0.70kg15 minute;

3), under the aseptic condition, add aseptic calf serum, packing ampere bottle, every bottle of about 5ml is stored in the refrigerator for subsequent use.