CN103013696A - Composition for cleaning medical instruments and preparation method of composition - Google Patents
Composition for cleaning medical instruments and preparation method of composition Download PDFInfo
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- CN103013696A CN103013696A CN 201210559704 CN201210559704A CN103013696A CN 103013696 A CN103013696 A CN 103013696A CN 201210559704 CN201210559704 CN 201210559704 CN 201210559704 A CN201210559704 A CN 201210559704A CN 103013696 A CN103013696 A CN 103013696A
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- stoste
- polyoxyethylene ether
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Abstract
The invention discloses a composition for cleaning medical instruments. The composition comprises 30% by mass of alkylphenol polyoxyethylene ether, 30% by mass of alkyl polyoxyethylene ether sodium sulfate, 30% by mass of fatty alcohol polyoxyethylene ether and 10% by mass of biological enzyme, wherein biological enzyme comprises 10%-30% by mass of cellulase, 5%-40% by mass of neutral protease, 10%-30% by mass of fungus Alpha-amylase, 5%-40% of lipase and 10%-30% of xylanase. The invention further discloses a preparation method of the composition, which comprises the steps as follows: respectively weighing five required enzymes, adding distilled water for dissolution, taking and filtering upper solutions and mixing the solutions in sequence to obtain biological enzyme; and adding water to mix alkyl phenol polyoxyethylene ether, alkyl polyoxyethylene ether sodium sulfate and fatty alcohol polyoxyethylene ether to obtain a solution, adding biological enzyme into the solution, and mixing biological enzyme and the solution to prepare the composition. The cleaning agent (namely the composition) provided by the invention can be used for fast removing fiber, bloodstain, protein, starch, sugar and fat on the medical instruments, and has a good decomposing effect and a stable cleaning effect.
Description
Technical field
The invention belongs to the cleaning product technical field, relate to the composition for the medicine equipment cleaning, the invention still further relates to the preparation method of composition.
Background technology
For a long time, clean-out system adopts the tensio-active agent of chemosynthesis, not only can cause environmental pollution, and its chemical residue can impact to environment.Medicine equipment cleans unclean, will cause infection, affects patient, medical personnel's health." hospital infection management way " passed in Ministry of Health's ministerial affairs meeting on June 15 in 2006 through discussion, advocates the antiseptic sterilization agent of using as medicine equipment with bio-enzyme cleaning agent.
The medicine equipment of valuable precision has many tiny hollow lacunas that have, and after instrument enters human body, is infected with many mucus secretions, blood etc., and common biological cleaner often can not be removed by the rapid osmotic dirt; And this class material very easily is adsorbed in the lacuna inwalls such as endoscope, sterilizing agent can not fully be arrived, and then cause and sterilize unsuccessfully; In addition, the protein substance in the common sterilizing agent again can with the material generation crosslinking reactions such as glutaraldehyde class, make it lose sterilization effect.And, the medical clean-out system that has is by starch, pineapple juice, vegetables oil, ammonium sulfate, the water fermentation substrate as the EM fungal component, cleaning Medical Instruments after the fermentation, contain amylase, lipase, proteolytic enzyme, cellulase and organic acid and various active thing in the meta-bolites after its fermentation, can Degradation be arranged to materials such as the blood on the medicine equipment, protein, carbohydrate, carbohydrate, fat.But this type of clean-out system is in the process of fermentation, and process parameter is not easy control, and its useless tunning is many, and the content of each enzyme is uncontrollable, causes its tunning cleaning effect unstable.
Summary of the invention
The purpose of this invention is to provide a kind of composition for the medicine equipment cleaning, can remove fast fiber, bloodstain, protein, starch, sugar, fat and inorganic pollutant on the medicine equipment, can not be bonded in the apparatus surface, decompose effectively, cleaning effect is stable.
Another object of the present invention provides the preparation method for the composition of medicine equipment cleaning.
The technical solution used in the present invention is, is used for the composition of medicine equipment cleaning, according to mass percent, formed by following raw material,
Alkylphenol polyoxyethylene 30%, alkyl polyoxyethylene ether sodium sulfate 30%,
Fatty alcohol-polyoxyethylene ether 30%, biological enzyme 10%,
Wherein, biological enzyme is comprised of following raw material according to mass percent,
Cellulase 10%-30%, neutral protease 5%-40%, fungal alpha-amylase 10%-30%,
Lipase 5%-40%, zytase 10%-30%, the mass percent summation that forms 5 kinds of raw materials of biological enzyme is 100%.
Another technical scheme that the present invention adopts is, is used for the preparation method of the composition of medicine equipment cleaning, specifically carry out according to following steps,
Step 1,
Take by weighing respectively the cellulase of 10%-30% according to mass percent, the neutral protease of 5%-40%, the fungal alpha-amylase of 10%-30%, the lipase of 5%-40%, the zytase of 10%-30%, the mass percent summation of above component is 100%; The solution of the cellulase, neutral protease, fungal alpha-amylase, lipase and the dissolving of zytase adding distil water that take by weighing being got top after 24 hours respectively filters with Büchner funnel, obtain respectively cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then the cellulase stoste after will filtering, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste are mixed successively, obtain biological enzyme;
Step 2,
Take by weighing 30% alkylphenol polyoxyethylene according to mass percent, 30% alkyl polyoxyethylene ether sodium sulfate, 30% fatty alcohol-polyoxyethylene ether, and 10% the biological enzyme that makes of step 1; First fatty alcohol-polyoxyethylene ether was stirred 30-40 minute at 75 ℃-80 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 17g/ml-30g/ml, then biological enzyme is joined in this solution and mix, and get final product.
The invention has the beneficial effects as follows, proteolytic enzyme, lipase, amylase, cellulase, carbohydrase are proportionally disposed, and the clean-out system that obtains is disposal facility surface Some Organic Pollutants, blood, mucus, fat and inorganic pollutant fast and efficiently.PH value is neutral, uses safety, has improved the efficient of cleaning, and has shortened disinfecting time, has protected apparatus, prolongs its work-ing life, is typical Green Chemistry treatment process.Be applicable to various medical endoscope, clean before the sterilization of laparoscopic surgery and tooth section apparatus.Hand washing, machine washing, ultrasonic wave are washed general, and soft water, hard water, normal temperature, heating all can be used.
The present invention also has following characteristics,
1. fast, each type organic on the safe cleaning device tool, comparing traditional manual cleaning has huge advantage.
2. biodegradable, belong to the environment protection ecological type scavenging solution, can directly enter in the sewage.
To apparatus without any corrodibility.
To human body skin without any hormesis.
5. soak time is short, and the joint part of apparatus is had lubrication.
Embodiment
The present invention is described in detail below in conjunction with embodiment.
The present invention is used for the composition of medicine equipment cleaning, according to mass percent, formed by following raw material,
Alkylphenol polyoxyethylene (TX-10) 30%, alkyl polyoxyethylene ether sodium sulfate (AES) 30%, fatty alcohol-polyoxyethylene ether (peregal 0-25) 30%, biological enzyme 10%,
Wherein, biological enzyme is comprised of following raw material according to mass percent,
Cellulase 10%-30%, neutral protease 5%-40%, fungal alpha-amylase 10%-30%,
Lipase 5%-40%, zytase 10%-30%, the mass percent summation that forms 5 kinds of raw materials of biological enzyme is 100%.
In composition of the present invention, alkylphenol polyoxyethylene is emulsifying agent;
Alkyl polyoxyethylene ether sodium sulfate is anion surfactant.
Fatty alcohol-polyoxyethylene ether is nonionogenic tenside.
Cellulase is the C1 enzyme, the mixture of β-Isosorbide-5-Nitrae dextranase and beta-glucan glycosides enzyme, and pH value is 7, plays the effect of hydrolysis VITAMIN.
Neutral protease, pH value is 7, is to refine the solid enzyme preparation that forms by subtilis through submerged fermentation, macromolecular proteolysis can be become the products such as amino acid under neutrallty condition.Neutral protease is the tawny powder, and temperature applicable range is 37 ℃-59 ℃, enzymic activity 50000u/g, 70000u/g.Can blood viscosity lowering, impel protein to be decomposed into peptide class and amino acid, play the effect of decomposing protein.
Fungal alpha-amylase is Isosorbide-5-Nitrae-α-D-glucan hydrolase, is to be made with extra care through operations such as deep layer cultivation, extractions by aspergillus oryzae Wa Er to form.This enzyme is endo-amylase, can be hydrolyzed rapidly the α of jelling starch, amylose starch and amylose water solution inside-Isosorbide-5-Nitrae glucoside bond.Fungal alpha-amylase is brown liquid, and the thermal adaptation scope is 45 ℃-65 ℃, enzymic activity 40000u/ml, smell free from extraneous odour, pH value 6.6.Effect is well with starch solution liquefaction and saccharification, protein hydrolysate.
Lipase is single enzyme, can degrade fat, glycerine and lipid acid.
Zytase be by Aspergillus niger strain through submerged fermentation, the product that cryodrying forms.It mainly decomposes the hemicellulose, the xylan that are polymerized by five-carbon sugar becomes five-carbon sugar, such as products such as wood sugar, xylo-bioses, xylotrioses, the pectinose of minority is arranged also.Outward appearance is light brown powder, 35 ℃-55 ℃ of temperature applicable ranges, free from extraneous odour, enzymic activity 5000u/g, 6000u/g, 8000u/g, 10000u/g, pH value 5.5.Can make the material organization softening, the cell walls hydrolysis reduces extract viscosity, effectively hydrolyzed xylan.Zytase can destroy fibrous tissue, and xylan is resolved into the enzyme of wood sugar, reduces the viscosity of material, reduces non-starch polysaccharide.
The present invention is used for the preparation method of composition of medicine equipment cleaning, specifically carries out according to following steps:
Step 1,
Take by weighing the cellulase of 10%-30% according to mass percent, the neutral protease of 5%-40%, the fungal alpha-amylase of 10%-30%, the lipase of 5%-40%, the zytase of 10%-30%, the mass percent summation of above component is 100%; The solution of the cellulase, neutral protease, fungal alpha-amylase, lipase and the dissolving of zytase adding distil water that take by weighing being got top after 24 hours respectively filters with Büchner funnel, obtain cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then the cellulase stoste after will filtering, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste are mixed successively, obtain biological enzyme;
Step 2,
Take by weighing 30% alkylphenol polyoxyethylene according to mass percent, 30% alkyl polyoxyethylene ether sodium sulfate, 30% fatty alcohol-polyoxyethylene ether, and 10% the biological enzyme that makes of step 1; First fatty alcohol-polyoxyethylene ether was stirred 30-40 minute at 75 ℃-80 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 17g/ml-30g/ml, then biological enzyme is joined in this solution and mix, namely obtain the bio-enzyme cleaning agent for medical apparatuses of inventing.
Composition of the present invention adds water with alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate and fatty alcohol-polyoxyethylene ether and is mixed to get solution, mixes with 5 kinds of enzymes, thereby has improved the activity of 5 kinds of enzymes, and the clean-out system shelf time that makes is long.Because alkyl polyoxyethylene ether sodium sulfate and fatty alcohol-polyoxyethylene ether itself are tensio-active agent just, so sanitising agent has the effect of better cleaning inorganic pollutant.Through lot of experiments, adopt the proportioning mode of each raw material among the present invention, can be so that various enzyme activities to be best in the sanitising agent, the shelf time is the longest.Among the preparation method of the present invention, preparation technology is simple, can be widely used in suitability for industrialized production.
Bio-enzyme cleaning agent using method of the present invention:
Clear water and clean-out system be according to the dilution of the mass ratio of 1:200-400, with instrument soaking to be cleaned in detergent solution 10-20 minute, and then gets final product with flushing with clean water.Result of use is best in 20 ℃ of-50 ℃ of scopes, and result of use is best between PH6.5-7.5.
Embodiment 1
Take by weighing 10% cellulase according to mass percent, 35% neutral protease, 10% fungal alpha-amylase, 35% lipase, 10% zytase, respectively with the cellulase that takes by weighing, neutral protease, fungal alpha-amylase, top is got in lipase and the dissolving of zytase adding distil water after 24 hours solution filters with Büchner funnel, obtain cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then the cellulase stoste after will filtering, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste is mixed successively, obtains biological enzyme; Take by weighing 30% alkylphenol polyoxyethylene according to mass percent, 30% alkyl polyoxyethylene ether sodium sulfate, 30% fatty alcohol-polyoxyethylene ether, and 10% biological enzyme; First fatty alcohol-polyoxyethylene ether was stirred 40 minutes at 75 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 17g/ml, then biological enzyme is joined in this solution and mix, and get final product.
Embodiment 2
Take by weighing 30% cellulase according to mass percent, 5% neutral protease, 30% fungal alpha-amylase, 5% lipase, 30% zytase, respectively with the cellulase that takes by weighing, neutral protease, fungal alpha-amylase, top is got in lipase and the dissolving of zytase adding distil water after 24 hours solution filters with Büchner funnel, obtain cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then the cellulase stoste after will filtering, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste is mixed successively, obtains biological enzyme; Take by weighing 30% alkylphenol polyoxyethylene according to mass percent, 30% alkyl polyoxyethylene ether sodium sulfate, 30% fatty alcohol-polyoxyethylene ether, and 10% biological enzyme; First fatty alcohol-polyoxyethylene ether was stirred 30 minutes at 80 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 30g/ml, then biological enzyme is joined in this solution and mix, and get final product.
Embodiment 3
Take by weighing 15% cellulase according to mass percent, 40% neutral protease, 15% fungal alpha-amylase, 15% lipase, 15% zytase, respectively with the cellulase that takes by weighing, neutral protease, fungal alpha-amylase, top is got in lipase and the dissolving of zytase adding distil water after 24 hours solution filters with Büchner funnel, obtain cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then the cellulase stoste after will filtering, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste is mixed successively, obtains biological enzyme; Take by weighing 30% alkylphenol polyoxyethylene according to mass percent, 30% alkyl polyoxyethylene ether sodium sulfate, 30% fatty alcohol-polyoxyethylene ether, and 10% biological enzyme; First fatty alcohol-polyoxyethylene ether was stirred 35 minutes at 78 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 18g/ml, then biological enzyme is joined in this solution and mix, and get final product.
Embodiment 4
Take by weighing 20% cellulase according to mass percent, 10% neutral protease, 10% fungal alpha-amylase, 40% lipase, 20% zytase, respectively with the cellulase that takes by weighing, neutral protease, fungal alpha-amylase, top is got in lipase and the dissolving of zytase adding distil water after 24 hours solution filters with Büchner funnel, obtain cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then the cellulase stoste after will filtering, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste is mixed successively, obtains biological enzyme; Take by weighing 30% alkylphenol polyoxyethylene according to mass percent, 30% alkyl polyoxyethylene ether sodium sulfate, 30% fatty alcohol-polyoxyethylene ether, and 10% biological enzyme; First fatty alcohol-polyoxyethylene ether was stirred 40 minutes at 75 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 25g/ml, then biological enzyme is joined in this solution and mix, and get final product.
Clean-out system according to embodiment 4 prepares detects outward appearance, pH value, smell, stability, effective content, enzyme activity, heavy metal content and arsenic content, and detected result is:
Outward appearance is the thickness transparent liquid, and liquid product is not stratified, no suspended substance or precipitation; PH value is 6.5; Natural smell free from extraneous odour; Liquid product is positioned in-3 ℃～-10 ℃ the refrigerator and placed 24 hours, takes out when returning to room temperature without crystallization, without precipitation, places 24 hours in 40 ℃ of insulation cans, and is not stratified, not muddy; Effective content is 18.48% in the sanitising agent, and enzyme activity is 19100u/g; Heavy metal content (concentration is in 1% solution, take lead note) is as 0.005mg/kg, arsenic content 0.003mg/kg.
The instruments that the clean-out system that embodiment 4 is made is used for cleaning postoperative, after the mass ratio dilution according to 1:200 of clear water and clean-out system instruments is placed clean-out system, soaked 10 minutes, then flushing with clean water, by the naked eye and fluorescent dye detect instruments surface clean, smooth, nontoxic.
In sum, fast and efficiently disposal facility surface Some Organic Pollutants, blood, mucus, fat and inorganic pollutant etc. of composition of the present invention.PH value is neutral, uses safety, has improved the efficient of cleaning, and has shortened disinfecting time, has protected apparatus, prolongs its work-ing life, is typical Green Chemistry treatment process.Be applicable to various medical endoscope, clean before the sterilization of laparoscopic surgery and tooth section apparatus.Hand washing, machine washing, ultrasonic wave are washed general, and soft water, hard water, normal temperature, heating all can be used.
Claims (2)
1. a composition that is used for the medicine equipment cleaning is characterized in that, according to mass percent, formed by following raw material,
Alkylphenol polyoxyethylene 30%, alkyl polyoxyethylene ether sodium sulfate 30%,
Fatty alcohol-polyoxyethylene ether 30%, biological enzyme 10%,
Wherein, described biological enzyme according to mass percent, is comprised of following raw material,
Cellulase 10%-30%, neutral protease 5%-40%, fungal alpha-amylase 10%-30%,
Lipase 5%-40%, zytase 10%-30%, the mass percent summation that forms 5 kinds of raw materials of biological enzyme is 100%.
2. be used for the preparation method of the composition of medicine equipment cleaning, it is characterized in that, specifically carry out according to following steps:
Step 1,
Take by weighing respectively the cellulase of 10%-30% according to mass percent, the neutral protease of 5%-40%, the fungal alpha-amylase of 10%-30%, the lipase of 5%-40%, the zytase of 10%-30%, the mass percent summation of above component is 100%; The solution of the cellulase, neutral protease, fungal alpha-amylase, lipase and the dissolving of zytase adding distil water that take by weighing being got top after 24 hours respectively filters with Büchner funnel, obtain respectively cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then the cellulase stoste after will filtering, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste are mixed successively, obtain biological enzyme;
Step 2,
Take by weighing 30% alkylphenol polyoxyethylene according to mass percent, 30% alkyl polyoxyethylene ether sodium sulfate, 30% fatty alcohol-polyoxyethylene ether, and 10% the biological enzyme that makes of step 1; First fatty alcohol-polyoxyethylene ether was stirred 30-40 minute at 75 ℃-80 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 17g/ml-30g/ml, then biological enzyme is joined in this solution and mix, and get final product.
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| CN 201210559704 CN103013696A (en) | 2012-12-20 | 2012-12-20 | Composition for cleaning medical instruments and preparation method of composition |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103666816A (en) * | 2013-12-05 | 2014-03-26 | 广东中测食品化妆品安全评价中心有限公司 | Biologic enzyme cleaning agent for fruit cleaning, and preparation method thereof |
| CN106893647A (en) * | 2017-02-28 | 2017-06-27 | 长安大学 | A kind of multienzyme medicine equipment cleaning agent and preparation method thereof |
| CN107841750A (en) * | 2017-11-07 | 2018-03-27 | 常州禾吉纺织品有限公司 | A kind of anti-rust metal detergent |
-
2012
- 2012-12-20 CN CN 201210559704 patent/CN103013696A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103666816A (en) * | 2013-12-05 | 2014-03-26 | 广东中测食品化妆品安全评价中心有限公司 | Biologic enzyme cleaning agent for fruit cleaning, and preparation method thereof |
| CN106893647A (en) * | 2017-02-28 | 2017-06-27 | 长安大学 | A kind of multienzyme medicine equipment cleaning agent and preparation method thereof |
| CN107841750A (en) * | 2017-11-07 | 2018-03-27 | 常州禾吉纺织品有限公司 | A kind of anti-rust metal detergent |
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Application publication date: 20130403 |