CN101921671B - Bio-enzyme cleaning agent for medical apparatuses and preparation method thereof - Google Patents
Bio-enzyme cleaning agent for medical apparatuses and preparation method thereof Download PDFInfo
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- CN101921671B CN101921671B CN2010102535510A CN201010253551A CN101921671B CN 101921671 B CN101921671 B CN 101921671B CN 2010102535510 A CN2010102535510 A CN 2010102535510A CN 201010253551 A CN201010253551 A CN 201010253551A CN 101921671 B CN101921671 B CN 101921671B
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Abstract
The invention discloses a bio-enzyme cleaning agent for medical apparatuses, which comprises the following components in percentage by weight: 19 percent of alkylphenol ethoxylate, 40 percent of alkyl polyoxyethylene ether sodium sulfate, 40 percent of fatty alcohol-polyoxyethylene ether and 1 percent of bio-enzyme, wherein the bio-enzyme comprises the following components in percentage by weight: 10-30 percent of cellulase, 5-40 percent of neutral protease, 10-30 percent of fungus alpha-amylase, 5-40 percent of lipase and 10-30 percent of xylanase. The invention also discloses a preparation method of the bio-enzyme cleaning agent, comprising the following steps of: respectively weighing the required five enzymes, adding distilled water for dissolving, taking and filtering upper solutions, and sequentially mixing the upper solutions to obtain the bio-enzyme; adding water to mix the alkylphenol ethoxylates, the alkyl polyoxyethylene ether sodium sulfate and the fatty alcohol-polyoxyethylene ether to obtain a solution; and then adding the bio-enzyme to the solution and mixing to obtain the bio-enzyme cleaning agent. The cleaning agent can be used for quickly removing fibers, bloodstains, protein, starch, sugar and fat on medical apparatuses and has the advantages of good decomposition effect and stable cleaning effect.
Description
Technical field
The invention belongs to the cleaning product technical field, relate to a kind of bio-enzyme cleaning agent for medical apparatuses, the invention still further relates to the preparation method of this clean-out system.
Background technology
For a long time, clean-out system adopts the tensio-active agent of chemosynthesis, not only can cause environmental pollution, and its chemical residue can impact to environment.Medicine equipment cleans unclean, will cause infection, affects patient, medical personnel's health." hospital infection management way " passed in Ministry of Health's ministerial affairs meeting on June 15 in 2006 through discussion, advocates the antiseptic sterilization agent of using as medicine equipment with bio-enzyme cleaning agent.
The medicine equipment of valuable precision has many tiny hollow lacunas that have, and after instrument enters human body, is infected with many mucus secretions, blood etc., and common biological cleaner often can not be removed by the rapid osmotic dirt; And this class material very easily is adsorbed in the lacuna inwalls such as endoscope, sterilizing agent can not be fully arrived, and then cause and sterilize unsuccessfully; In addition, the protein substance in common sterilizing agent again can with the material generation crosslinking reactions such as glutaraldehyde class, make it lose sterilization effect.And, the medical clean-out system had is the fermentation substrate as the EM fungal component by starch, pineapple juice, vegetables oil, ammonium sulfate, water, clean Medical Instruments after fermentation, contain amylase, lipase, proteolytic enzyme, cellulase and organic acid and various active thing in meta-bolites after its fermentation, can Degradation be arranged to materials such as the blood on medicine equipment, protein, carbohydrate, carbohydrate, fat.But this type of clean-out system is in the process of fermentation, process parameter is not easy to control, and its useless tunning is many, and the content of each enzyme is uncontrollable, causes its tunning cleaning effect unstable.
Summary of the invention
The purpose of this invention is to provide a kind of bio-enzyme cleaning agent for medical apparatuses, can remove fast fiber, bloodstain, protein, starch, sugar, fat and inorganic pollutant on medicine equipment, can not be bonded in the apparatus surface, decompose effectively, cleaning effect is stable.
Another object of the present invention is to provide the preparation method of this bio-enzyme cleaning agent.
The technical solution used in the present invention is, bio-enzyme cleaning agent for medical apparatuses, according to mass percent, be comprised of following raw material,
Alkylphenol polyoxyethylene 19%, alkyl polyoxyethylene ether sodium sulfate 40%,
Fatty alcohol-polyoxyethylene ether 40%, biological enzyme 1%,
Wherein, biological enzyme, according to mass percent, is comprised of following raw material,
Cellulase 10%-30%, neutral protease 5%-40%, fungal alpha-amylase 10%-30%,
Lipase 5%-40%, zytase 10%-30%, the mass percent summation that forms 5 kinds of raw materials of biological enzyme is 100%.
Another technical scheme that the present invention adopts is, the preparation method of this bio-enzyme cleaning agent, specifically carry out according to following steps,
Step 1,
Take respectively the cellulase of 10%-30% according to mass percent, the neutral protease of 5%-40%, the fungal alpha-amylase of 10%-30%, the lipase of 5%-40%, the zytase of 10%-30%, the mass percent summation of above component is 100% respectively by taken cellulase, neutral protease, fungal alpha-amylase, lipase and zytase adding distil water dissolve the solution of getting top after 24 hours and filter with Büchner funnel, obtain respectively cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then the cellulase stoste after filtering, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste is mixed successively, obtain biological enzyme,
Step 2,
Take 19% alkylphenol polyoxyethylene according to mass percent, 40% alkyl polyoxyethylene ether sodium sulfate, 40% fatty alcohol-polyoxyethylene ether, and 1% the biological enzyme that makes of step 1; First fatty alcohol-polyoxyethylene ether is stirred to 30-40 minute at 75 ℃-80 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 17g/ml-30g/ml, then biological enzyme is joined in this solution and mixes, obtain.
The invention has the beneficial effects as follows, proteolytic enzyme, lipase, amylase, cellulase, carbohydrase are proportionally configured, and the clean-out system obtained is disposal facility surface Some Organic Pollutants, blood, mucus, fat and inorganic pollutant fast and efficiently.PH value neutrality, used safety, improved the efficiency of cleaning, and shortened disinfecting time, protected apparatus, extends its work-ing life, is typical Green Chemistry treatment process.Be applicable to various medical endoscope, clean before the sterilization of laparoscopic surgery and gear division apparatus.Hand washing, machine washing, ultrasonic wave are washed general, and soft water, hard water, normal temperature, heating all can be used.
The present invention also has following characteristics,
1. fast, each type organic on the safe cleaning device tool, comparing traditional manual cleaning has huge advantage.
2. biodegradable, belong to the environment protection ecological type scavenging solution, can directly enter in sewage.
To apparatus without any corrodibility.
To human body skin without any hormesis.
5. soak time is short, and the joint part of apparatus is had to lubrication.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
Bio-enzyme cleaning agent for medical apparatuses of the present invention, according to mass percent, be comprised of following raw material,
Alkylphenol polyoxyethylene (TX-10) 19%, alkyl polyoxyethylene ether sodium sulfate (AES) 40%, fatty alcohol-polyoxyethylene ether (peregal 0-25) 40%, biological enzyme 1%,
Wherein, biological enzyme, according to mass percent, is comprised of following raw material,
Cellulase 10%-30%, neutral protease 5%-40%, fungal alpha-amylase 10%-30%,
Lipase 5%-40%, zytase 10%-30%, the mass percent summation that forms 5 kinds of raw materials of biological enzyme is 100%.
In bio-enzyme cleaning agent of the present invention, alkylphenol polyoxyethylene is emulsifying agent;
Alkyl polyoxyethylene ether sodium sulfate is anion surfactant.
Fatty alcohol-polyoxyethylene ether is nonionogenic tenside.
Cellulase is the C1 enzyme, the mixture of β-Isosorbide-5-Nitrae dextranase and beta-glucan glycosides enzyme, and pH value is 7.
Neutral protease, pH value is 7, is to be refined the solid enzyme preparation formed through submerged fermentation by subtilis, under neutrallty condition, macromolecular proteolysis can be become to the products such as amino acid.Neutral protease is the tawny powder, and temperature applicable range is 37 ℃-59 ℃, enzymic activity 50000u/g, 70000u/g.Can reduce blood viscosity, impel protein to be decomposed into peptide class and amino acid, play the effect of decomposing protein.
Fungal alpha-amylase is Isosorbide-5-Nitrae-α-D-glucan hydrolase, is to be made with extra care and form through operations such as deep layer cultivation, extractions by aspergillus oryzae Wa Er.This enzyme is endo-amylase, can be hydrolyzed rapidly the α of jelling starch, amylose starch and amylose water solution inside-Isosorbide-5-Nitrae glucoside bond.Fungal alpha-amylase is brown liquid, and the thermal adaptation scope is 45 ℃-65 ℃, enzymic activity 40000u/ml, smell free from extraneous odour, pH value 6.6.Effect is well by starch solution liquefaction and saccharification, protein hydrolysate.
Lipase is single enzyme, can degrade fat, glycerine and lipid acid.
Zytase be by Aspergillus niger strain through submerged fermentation, the product that cryodrying forms.It mainly decomposes the hemicellulose, the xylan that are polymerized by five-carbon sugar becomes five-carbon sugar, as products such as wood sugar, xylo-bioses, xylotrioses, the pectinose of minority is also arranged.Outward appearance is light brown powder, 35 ℃-55 ℃ of temperature applicable ranges, free from extraneous odour, enzymic activity 5000u/g, 6000u/g, 8000u/g, 10000u/g, pH value 5.5.Can make the material organization softening, the cell walls hydrolysis, reduce extract viscosity, effectively hydrolyzed xylan.Zytase can destroy fibrous tissue, xylan is resolved into to the enzyme of wood sugar, reduces the viscosity of material, reduces non-starch polysaccharide.
The preparation method of bio-enzyme cleaning agent for medical apparatuses of the present invention, specifically carry out according to following steps:
Step 1,
Take the cellulase of 10%-30% according to mass percent, the neutral protease of 5%-40%, the fungal alpha-amylase of 10%-30%, the lipase of 5%-40%, the zytase of 10%-30%, the mass percent summation of above component is 100%; Respectively taken cellulase, neutral protease, fungal alpha-amylase, lipase and zytase adding distil water being dissolved to the solution of getting top after 24 hours filters with Büchner funnel, obtain cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste after filtering are mixed successively, obtain biological enzyme;
Step 2,
Take 19% alkylphenol polyoxyethylene according to mass percent, 40% alkyl polyoxyethylene ether sodium sulfate, 40% fatty alcohol-polyoxyethylene ether, and 1% the biological enzyme that makes of step 1; First fatty alcohol-polyoxyethylene ether is stirred to 30-40 minute at 75 ℃-80 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 17g/ml-30g/ml, then biological enzyme is joined in this solution and mixes, obtain the bio-enzyme cleaning agent for medical apparatuses of invention.
Bio-enzyme cleaning agent of the present invention, alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate and fatty alcohol-polyoxyethylene ether are added to water and be mixed to get solution, mixed with 5 kinds of enzymes, thereby improved the activity of 5 kinds of enzymes, the clean-out system shelf time made is long.Because alkyl polyoxyethylene ether sodium sulfate and fatty alcohol-polyoxyethylene ether itself be just tensio-active agent, so sanitising agent has the effect that better cleans inorganic pollutant.Through lot of experiments, adopt the proportioning mode of each raw material in the present invention, can be so that in sanitising agent, the vigor of various enzymes to be best, the shelf time is the longest.In preparation method of the present invention, preparation technology is simple, can be widely used in suitability for industrialized production.
Bio-enzyme cleaning agent using method of the present invention:
Clear water and clean-out system are according to 1: the mass ratio dilution of 200-400, and by instrument soaking to be cleaned 10-20 minute in detergent solution, and then rinse and get final product with clear water.In 20 ℃ of-50 ℃ of scopes, result of use is best, and between PH6.5-7.5, result of use is best.
Embodiment 1
Take 10% cellulase according to mass percent, 35% neutral protease, 10% fungal alpha-amylase, 35% lipase, 10% zytase, respectively by taken cellulase, neutral protease, fungal alpha-amylase, lipase and zytase adding distil water dissolve the solution of getting top after 24 hours and filter with Büchner funnel, obtain cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then the cellulase stoste after filtering, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste is mixed successively, obtain biological enzyme, take 19% alkylphenol polyoxyethylene according to mass percent, 40% alkyl polyoxyethylene ether sodium sulfate, 40% fatty alcohol-polyoxyethylene ether, and 1% biological enzyme, first fatty alcohol-polyoxyethylene ether is stirred 40 minutes at 75 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 17g/ml, then biological enzyme is joined in this solution and mixes, obtain.
Embodiment 2
Take 30% cellulase according to mass percent, 5% neutral protease, 30% fungal alpha-amylase, 5% lipase, 30% zytase, respectively by taken cellulase, neutral protease, fungal alpha-amylase, lipase and zytase adding distil water dissolve the solution of getting top after 24 hours and filter with Büchner funnel, obtain cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then the cellulase stoste after filtering, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste is mixed successively, obtain biological enzyme, take 19% alkylphenol polyoxyethylene according to mass percent, 40% alkyl polyoxyethylene ether sodium sulfate, 40% fatty alcohol-polyoxyethylene ether, and 1% biological enzyme, first fatty alcohol-polyoxyethylene ether is stirred 30 minutes at 80 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 30g/ml, then biological enzyme is joined in this solution and mixes, obtain.
Embodiment 3
Take 15% cellulase according to mass percent, 40% neutral protease, 15% fungal alpha-amylase, 15% lipase, 15% zytase, respectively by taken cellulase, neutral protease, fungal alpha-amylase, lipase and zytase adding distil water dissolve the solution of getting top after 24 hours and filter with Büchner funnel, obtain cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then the cellulase stoste after filtering, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste is mixed successively, obtain biological enzyme, take 19% alkylphenol polyoxyethylene according to mass percent, 40% alkyl polyoxyethylene ether sodium sulfate, 40% fatty alcohol-polyoxyethylene ether, and 1% biological enzyme, first fatty alcohol-polyoxyethylene ether is stirred 35 minutes at 78 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 18g/ml, then biological enzyme is joined in this solution and mixes, obtain.
Embodiment 4
Take 20% cellulase according to mass percent, 10% neutral protease, 10% fungal alpha-amylase, 40% lipase, 20% zytase, respectively by taken cellulase, neutral protease, fungal alpha-amylase, lipase and zytase adding distil water dissolve the solution of getting top after 24 hours and filter with Büchner funnel, obtain cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then the cellulase stoste after filtering, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste is mixed successively, obtain biological enzyme, take 19% alkylphenol polyoxyethylene according to mass percent, 40% alkyl polyoxyethylene ether sodium sulfate, 40% fatty alcohol-polyoxyethylene ether, and 1% biological enzyme, first fatty alcohol-polyoxyethylene ether is stirred 40 minutes at 75 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 25g/ml, then biological enzyme is joined in this solution and mixes, obtain.
The clean-out system prepared according to embodiment 4, detected outward appearance, pH value, smell, stability, effective content, enzyme activity, heavy metal content and arsenic content, and detected result is:
Outward appearance is the thickness transparent liquid, and liquid product is not stratified, no suspended substance or precipitation; PH value is 6.5; Natural smell free from extraneous odour; Liquid product is positioned in the refrigerator of-3 ℃~-10 ℃ and places 24 hours, takes out while returning to room temperature without crystallization, without precipitation, in 40 ℃ of insulation cans, places 24 hours, not stratified, not muddy; In sanitising agent, effective content is 18.48%, and enzyme activity is 19100u/g; Heavy metal content (concentration is in 1% solution, the lead of take note) is 0.005mg/kg, arsenic content 0.003mg/kg.
The clean-out system that embodiment 4 is made is for cleaning postoperative instruments, clear water and clean-out system are placed in clean-out system by instruments after according to the mass ratio of 1: 200 dilution, soak 10 minutes, then clear water rinses, by the naked eye and fluorescent dye detect, instruments surface clean, smooth, nontoxic.
In sum, sanitising agent of the present invention disposal facility surface Some Organic Pollutants, blood, mucus, fat and inorganic pollutant etc. fast and efficiently.PH value neutrality, used safety, improved the efficiency of cleaning, and shortened disinfecting time, protected apparatus, extends its work-ing life, is typical Green Chemistry treatment process.Be applicable to various medical endoscope, clean before the sterilization of laparoscopic surgery and gear division apparatus.Hand washing, machine washing, ultrasonic wave are washed general, and soft water, hard water, normal temperature, heating all can be used.
Claims (2)
1. a bio-enzyme cleaning agent for medical apparatuses, is characterized in that, according to mass percent, by following raw material, formed,
Alkylphenol polyoxyethylene 19%, alkyl polyoxyethylene ether sodium sulfate 40%,
Fatty alcohol-polyoxyethylene ether 40%, biological enzyme 1%,
Wherein, described biological enzyme, according to mass percent, be comprised of following raw material,
Cellulase 10%-30%, neutral protease 5%-40%, fungal alpha-amylase 10%-30%,
Lipase 5%-40%, zytase 10%-30%, the mass percent summation that forms 5 kinds of raw materials of biological enzyme is 100%.
2. the preparation method of a bio-enzyme cleaning agent for medical apparatuses as claimed in claim 1, is characterized in that, specifically according to following steps, carries out:
Step 1,
Take respectively the cellulase of 10%-30% according to mass percent, the neutral protease of 5%-40%, the fungal alpha-amylase of 10%-30%, the lipase of 5%-40%, the zytase of 10%-30%, the mass percent summation of above component is 100%; Respectively taken cellulase, neutral protease, fungal alpha-amylase, lipase and zytase adding distil water being dissolved to the solution of getting top after 24 hours filters with Büchner funnel, obtain respectively cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste and zytase stoste, then cellulase stoste, neutral protease stoste, fungal alpha-amylase stoste, lipase stoste, zytase stoste after filtering are mixed successively, obtain biological enzyme;
Step 2,
Take 19% alkylphenol polyoxyethylene according to mass percent, 40% alkyl polyoxyethylene ether sodium sulfate, 40% fatty alcohol-polyoxyethylene ether, and 1% the biological enzyme that makes of step 1; First fatty alcohol-polyoxyethylene ether is stirred to 30-40 minute at 75 ℃-80 ℃, then will claim alkylphenol polyoxyethylene, alkyl polyoxyethylene ether sodium sulfate add water with fatty alcohol-polyoxyethylene ether and fully mix, obtain the solution that concentration is 17g/ml-30g/ml, then biological enzyme is joined in this solution and mixes, obtain.
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CN102294056B (en) * | 2011-09-02 | 2013-12-04 | 山东新华医疗器械股份有限公司 | Surgical instrument moisturizing agent and preparation method thereof |
CN103087834A (en) * | 2011-11-04 | 2013-05-08 | 上海利康消毒高科技有限公司 | Pre-cleaning and moisturizing composition of medical apparatuses and method of preparing disinfectant by using same |
CN103865663B (en) * | 2012-12-12 | 2016-09-07 | 深圳市芭格美生物科技有限公司 | Multienzyme cleaning agent for medical purpose and preparation method thereof |
CN103642601A (en) * | 2013-12-04 | 2014-03-19 | 青岛安信医疗器械有限公司 | Bio-enzyme cleaner for medical devices |
CN104073368A (en) * | 2013-12-05 | 2014-10-01 | 成都老肯科技股份有限公司 | Multi-enzyme cleaning solution suitable for hand-washing medical equipment and preparation method of multi-enzyme cleaning solution |
CN106010807B (en) * | 2016-06-08 | 2018-05-18 | 大连莱恩医疗工业有限公司 | A kind of multienzyme cleaning agent for stain on medical instrument |
CN106893647A (en) * | 2017-02-28 | 2017-06-27 | 长安大学 | A kind of multienzyme medicine equipment cleaning agent and preparation method thereof |
CN111410864A (en) * | 2020-04-24 | 2020-07-14 | 西安理工大学 | Lipase type water-based ink cleaning agent and preparation method thereof |
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EP1294410A1 (en) * | 2000-04-07 | 2003-03-26 | Novapharm Research (Australia) Pty. Limited | Process and composition for cleaning medical instruments |
CN101077999A (en) * | 2006-05-25 | 2007-11-28 | 常锋 | Method for preparing multienzyme cleaning agent for medical purpose |
WO2008040818A1 (en) * | 2006-10-06 | 2008-04-10 | Novozymes A/S | Detergent compositions and the use of enzyme combinations therein |
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