CN103005138A - Process of extracting sea cucumber peptides - Google Patents
Process of extracting sea cucumber peptides Download PDFInfo
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- CN103005138A CN103005138A CN2012105469346A CN201210546934A CN103005138A CN 103005138 A CN103005138 A CN 103005138A CN 2012105469346 A CN2012105469346 A CN 2012105469346A CN 201210546934 A CN201210546934 A CN 201210546934A CN 103005138 A CN103005138 A CN 103005138A
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- sea cucumber
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- cucumber peptides
- peptides
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Abstract
The invention discloses a process of extracting sea cucumber peptides, which comprises the following steps: (1) carrying out pretreatment by cleaning fresh sea cucumbers, removing viscera and drying; (2) performing micronization on the pretreated sea cucumbers to be powder of 200 to 300 mesh; (3) digesting by adding peptide molecule enzymes 10 to 20 g in per 1000 g powder and deactivating the enzymes 30 to 60 min later; (4) performing ultrafiltration by using a conventional ultrafilter; (5) performing spray-drying at room temperature; and (6) performing vacuum qualitative treatment at 70 DEG C to 80 DEG C to obtain sea cucumber peptides. Compared with the current process of extracting sea cucumber peptides, the process disclosed by the invention has higher purity of extraction and prevents the loss of nutrients.
Description
Technical field
The present invention relates to a kind of extracting method of sea cucumber bio preparation, be specifically related to a kind of extraction process of sea cucumber peptide.
Background technology
Sea cucumber peptide is to utilize the modern biology technology, having of adopting that the technology such as enzymolysis, extraction, ultrafiltration, purifying extract regulated blood fat, immunological regulation, antitumor, the natural bioactivity substance of regulating the effects such as endocrine, cosmetology, acid mucopolysaccharide with anticoagulation, reducing blood lipid, the effect of reduction blood viscosity, from wall of sea cucumber Stichopus japonicus and sea cucumber intestine, extract, and the sea cucumber peptide nutritional labeling that the extraction process of existing sea cucumber peptide is extracted runs off easily, causes can not get due tonic effect.
Summary of the invention
Goal of the invention: the present invention provides a kind of extraction process of sea cucumber peptide in order to solve the deficiencies in the prior art.
Technical scheme: in order to realize the foregoing invention purpose, the technical solution used in the present invention is:
A kind of extraction process of sea cucumber peptide comprises the following steps:
(1) preliminary treatment: bright sea cucumber is cleaned, gills, and oven dry;
(2) will carry out the ultramicro grinding powdered through pretreated sea cucumber, to the 200-300 order;
(3) every kilogram of powder adds peptide molecule enzymolysis 10-20g, and enzyme goes out after the 30-60min;
(4) carry out ultrafiltration with the conventional ultrafiltration device;
(5) carry out spray-drying under the normal temperature;
(6) under 70-80 ℃, carry out the qualitative processing of vacuum, sea cucumber peptide.
Beneficial effect: compared with prior art, the sea cucumber peptide purity that the present invention extracts is higher, and tonic effect is better.
The specific embodiment
The present invention will be described in detail below in conjunction with specific embodiment.
Embodiment 1
A kind of extraction process of sea cucumber peptide comprises the following steps:
(1) preliminary treatment: bright sea cucumber is cleaned, gills, and oven dry;
(2) will carry out the ultramicro grinding powdered through pretreated sea cucumber, to 200 orders;
(3) every kilogram of powder adds peptide molecule enzymolysis 10g, and enzyme goes out after the 30min;
(4) carry out ultrafiltration with the conventional ultrafiltration device;
(5) carry out spray-drying under the normal temperature;
(6) under 70 ℃, carry out the qualitative processing of vacuum, sea cucumber peptide.
Embodiment 2
A kind of extraction process of sea cucumber peptide comprises the following steps:
(1) preliminary treatment: bright sea cucumber is cleaned, gills, and oven dry;
(2) will carry out the ultramicro grinding powdered through pretreated sea cucumber, to 300 orders;
(3) every kilogram of powder adds peptide molecule enzymolysis 20g, and enzyme goes out after the 60min;
(4) carry out ultrafiltration with the conventional ultrafiltration device;
(5) carry out spray-drying under the normal temperature;
(6) under 80 ℃, carry out the qualitative processing of vacuum, sea cucumber peptide.
Embodiment 3
A kind of extraction process of sea cucumber peptide comprises the following steps:
(1) preliminary treatment: bright sea cucumber is cleaned, gills, and oven dry;
(2) will carry out the ultramicro grinding powdered through pretreated sea cucumber, to 250 orders;
(3) every kilogram of powder adds peptide molecule enzymolysis 15g, and enzyme goes out after the 50min;
(4) carry out ultrafiltration with the conventional ultrafiltration device;
(5) carry out spray-drying under the normal temperature;
(6) under 75 ℃, carry out the qualitative processing of vacuum, sea cucumber peptide.
Claims (1)
1. the extraction process of a sea cucumber peptide is characterized in that: comprise the following steps:
(1) preliminary treatment: bright sea cucumber is cleaned, gills, and oven dry;
(2) will carry out the ultramicro grinding powdered through pretreated sea cucumber, to the 200-300 order;
(3) every kilogram of powder adds peptide molecule enzymolysis 10-20g, and enzyme goes out after the 30-60min;
(4) carry out ultrafiltration with the conventional ultrafiltration device;
(5) carry out spray-drying under the normal temperature;
(6) under 70-80 ℃, carry out the qualitative processing of vacuum, sea cucumber peptide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105469346A CN103005138A (en) | 2012-12-17 | 2012-12-17 | Process of extracting sea cucumber peptides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105469346A CN103005138A (en) | 2012-12-17 | 2012-12-17 | Process of extracting sea cucumber peptides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103005138A true CN103005138A (en) | 2013-04-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN2012105469346A Pending CN103005138A (en) | 2012-12-17 | 2012-12-17 | Process of extracting sea cucumber peptides |
Country Status (1)
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| CN (1) | CN103005138A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103243127A (en) * | 2013-04-19 | 2013-08-14 | 中国水稻研究所 | Method for integrally preparing compound amino acid by using superfine grinding enzymolysis |
| CN103468775A (en) * | 2013-06-05 | 2013-12-25 | 青岛耐特生物技术有限公司 | Method for extracting high-purity sea cucumber polypeptides |
| CN104544454A (en) * | 2014-12-19 | 2015-04-29 | 渤海大学 | Sea cucumber peptide nutrition powder and preparation method thereof |
| CN108208306A (en) * | 2017-12-15 | 2018-06-29 | 黑龙江珍宝岛药业股份有限公司 | A kind of sea cucumber peptide extraction process and its application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101191139A (en) * | 2007-04-28 | 2008-06-04 | 付学军 | Integrated extraction technique for sea cucumber polypeptide and polysaccharide |
| CN101406295A (en) * | 2008-09-08 | 2009-04-15 | 赵兴坤 | Total nutrient silkworm chrysalis peptide functional foodstuff and preparation method thereof |
| CN102488073A (en) * | 2011-12-29 | 2012-06-13 | 吴庆林 | Extraction method of sea cucumber polypeptide |
| CN102550797A (en) * | 2012-03-02 | 2012-07-11 | 大连海洋大学 | Production method of sea cucumber peptide extract |
-
2012
- 2012-12-17 CN CN2012105469346A patent/CN103005138A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101191139A (en) * | 2007-04-28 | 2008-06-04 | 付学军 | Integrated extraction technique for sea cucumber polypeptide and polysaccharide |
| CN101406295A (en) * | 2008-09-08 | 2009-04-15 | 赵兴坤 | Total nutrient silkworm chrysalis peptide functional foodstuff and preparation method thereof |
| CN102488073A (en) * | 2011-12-29 | 2012-06-13 | 吴庆林 | Extraction method of sea cucumber polypeptide |
| CN102550797A (en) * | 2012-03-02 | 2012-07-11 | 大连海洋大学 | Production method of sea cucumber peptide extract |
Non-Patent Citations (1)
| Title |
|---|
| 李勇: "《肽营养学》", 31 December 2007, 北京大学医学出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103243127A (en) * | 2013-04-19 | 2013-08-14 | 中国水稻研究所 | Method for integrally preparing compound amino acid by using superfine grinding enzymolysis |
| CN103243127B (en) * | 2013-04-19 | 2015-02-04 | 中国水稻研究所 | Method for integrally preparing compound amino acid by using superfine grinding enzymolysis |
| CN103468775A (en) * | 2013-06-05 | 2013-12-25 | 青岛耐特生物技术有限公司 | Method for extracting high-purity sea cucumber polypeptides |
| CN104544454A (en) * | 2014-12-19 | 2015-04-29 | 渤海大学 | Sea cucumber peptide nutrition powder and preparation method thereof |
| CN108208306A (en) * | 2017-12-15 | 2018-06-29 | 黑龙江珍宝岛药业股份有限公司 | A kind of sea cucumber peptide extraction process and its application |
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Application publication date: 20130403 |