CN102994608A - Identification diagnostic agent for pathogenic bacteria using citrate in nosocomial infection - Google Patents

Abstract

The invention relates to an identification diagnostic agent for pathogenic bacteria using citrate in nosocomial infection. The major components include sodium citrate and the like, and the identification diagnostic agent is prepared by certain technology. The identification diagnostic agent is used for identifying whether bacteria can use citrate. The biochemical reaction test is used for identifying the type of the pathogenic bacteria using citrate in nosocomial infection, and has the key performances of accuracy, high speed and convenience.

Description

Hospital infection utilizes citrate pathogenic bacterium differential diagnosis agent

Technical field the present invention relates to a kind of hospital infection and utilizes citrate pathogenic bacterium differential diagnosis agent, its main component is Sodium Citrate, sodium-chlor, sal epsom, potassium primary phosphate, primary ammonium phosphate, bromothymol blue, yeast extract, agar, makes through certain technique.Can utilize citrate in order to discriminating bacteria.Utilize the kind of biochemical reaction test discriminating bacteria, it is accurate, quick, easy that its key is.Because different bacterium has its unique enzyme system separately, thereby the decomposition that produces in metabolic process is also different with synthetic meta-bolites, these decomposition or synthetic meta-bolites respectively have again different Biochemical Characteristics, recycle the biochemical reaction that its different Biochemical Characteristics produce under corresponding culture condition, carry out the discriminating of bacterial species, this is the most frequently used means of present Microbiological Lab.Because at present the range of application of culture condition and indicator is limit, the observation bacterium exists to the discriminating differential diagnosis agent of organic acid salt and ammonium salt utilization that required inoculum size is large, incubation time is long, indicator discoloration is not obvious, be prone to the problems such as false positive or false negative in the biochemical reaction test.Can it be to observe bacterium to a comprehensive differential diagnosis agent of organic acid salt and ammonium salt utilization that hospital infection utilizes citrate pathogenic bacterium differential diagnosis agent, utilize citrate in order to discriminating bacteria, thereby further judge kind and the Biochemical Characteristics of bacterium.Along with the progress of biological study, both at home and abroad to the research of discrimination of bacteria also in continuous development.Biological worker wishes to have a kind of differential diagnosis agent that can overcome above-mentioned deficiency always---and hospital infection utilizes citrate pathogenic bacterium differential diagnosis agent.

Background technology the object of the present invention is to provide a kind of hospital infection that can overcome existing differential diagnosis agent deficiency to utilize citrate pathogenic bacterium differential diagnosis agent.Differential diagnosis agent similar with other compared possesses following characteristics: required inoculum size is little, and the alkali variable color can appear producing in a small amount of bacterium; Indicator discoloration is obvious fast, gets final product observations in 24 hours; Be not prone to false positive, only for the bacterium that can utilize Sodium Citrate can grow, variable color; Be not prone to false negative, can destroy the antibacterial substance that exists in the sample, bacterial growth is not suppressed; Test positive control bacterium increases the bacterium breeding rapidly in this solid differential diagnosis agent, and product alkali makes differential diagnosis agent variable color.

Summary of the invention main points of the present invention are to select suitable component, and carry out rational mixture, through heat, dissolve, the technique such as mixing, cooling, packing, high-temperature sterilization forms a kind of hospital infection and utilizes citrate pathogenic bacterium differential diagnosis agent.

The prescription following (component and weight percent % thereof) that the present invention selects: Sodium Citrate 0.8-0.9; Sodium-chlor 0.8-0.85; Sal epsom 0.01-0.03; Potassium primary phosphate 0.08-0.12; Primary ammonium phosphate 0.13-0.15; Bromothymol blue 0.3-0.5; Yeast extract 3.5-4.0; Agar 1.5-2.5; Distilled water adds to 100.

Sodium Citrate is unique source of carbon in the differential diagnosis agent, and primary ammonium phosphate is unique source of nitrogen in the differential diagnosis agent.Can utilize Sodium Citrate can grow in the differential diagnosis agent for the bacterium of carbon source, and decompose citrate and produce at last carbonate, make the differential diagnosis agent become alkalescence, the bromothymol blue indicator in differential diagnosis agent this moment becomes mazarine by green.Sal epsom can destroy the bacteriostatic action of the tsiklomitsin, duomycin, terramycin, Liu Suanyan NEOMYCIN SULPHATE, polymyxin and the Streptomycin sulphate that exist in the sample; Sodium-chlor is used for adjusting osmotic pressure; Potassium primary phosphate is used for regulating pH.Yeast extract, agar are the nutrition in the differential diagnosis agent and support composition; Test positive control bacterium increases the bacterium breeding in this solid differential diagnosis agent, and product alkali makes differential diagnosis agent variable color.

The preparation method of product of the present invention is:

1), Sodium Citrate, sodium-chlor, sal epsom, potassium primary phosphate, primary ammonium phosphate, agar are added in the distilled water in proportion, reheats dissolving, correct pH to 6.8, filter with flannelette;

2) add, again the bromothymol blue mixing, pressuresteam sterilization 1.05kg15 minute;

3), under the aseptic condition, add aseptic yeast extract, the packing test tube, every pipe is 2ml approximately, will contain while hot the sterilizing test tubes inclination certain angle of differential diagnosis agent, and bevel after the condensation is stand-by.

The present invention has the following advantages: (1) required inoculum size is little, and the alkali variable color can appear producing in a small amount of bacterium; (2) indicator discoloration is obvious fast, gets final product observations in 24 hours; (3) be not prone to false positive, only for the bacterium that can utilize Sodium Citrate can grow, variable color; (4) be not prone to false negative, sal epsom can destroy the bacteriostatic action of the tsiklomitsin, duomycin, terramycin, Liu Suanyan NEOMYCIN SULPHATE, polymyxin and the Streptomycin sulphate that exist in the sample; (5) test positive control bacterium increases the bacterium breeding rapidly in this differential diagnosis agent, and product alkali makes differential diagnosis agent variable color.

The present invention is described in further detail below in conjunction with embodiment for embodiment:

Be configured according to following table institute column data (weight percent %) and described step thereof:

Prescription one:

Prescription two:

。

Claims (2)

1. the present invention relates to a kind of hospital infection and utilize citrate pathogenic bacterium differential diagnosis agent, it is characterized in that having following prescription (component and weight percent % thereof): Sodium Citrate 0.8-0.9; Sodium-chlor 0.8-0.85; Sal epsom 0.01-0.03; Potassium primary phosphate 0.08-0.12; Primary ammonium phosphate 0.13-0.15; Bromothymol blue 0.3-0.5; Yeast extract 3.5-4.0; Agar 1.5-2.5; Distilled water adds to 100.

2. the described hospital infection of claim 1 utilizes citrate pathogenic bacterium differential diagnosis agent, it is characterized in that having the method for being prepared as follows:

1), Sodium Citrate, sodium-chlor, sal epsom, potassium primary phosphate, primary ammonium phosphate, agar are added in the distilled water in proportion, reheats dissolving, correct pH to 6.8, filter with flannelette;

2) add, again the bromothymol blue mixing, pressuresteam sterilization 1.05kg15 minute;

3), under the aseptic condition, add aseptic yeast extract, the packing test tube, every pipe is 2ml approximately, will contain while hot the sterilizing test tubes inclination certain angle of differential diagnosis agent, and bevel after the condensation is stand-by.