CN101463381A - Hexadecane trimethyl ammonium bromide neutralization agent - Google Patents

Abstract

The invention relates to a hexadecyl trimethyl ammonium bromide neutralizing agent. The main components comprise beef extract, peptone, sodium chloride, agar powder and hexadecyl trimethyl ammonium bromide which are processed to obtain the neutralizing agent by certain technology. The hexadecyl trimethyl ammonium bromide neutralizing agent is used for neutralizing, isolating and culturing an ordinary sample or a patient sample which is applied with antibiotic and sulfa drug. Compared with other similar culture mediums, the hexadecyl trimethyl ammonium bromide neutralizing agent has the characteristics as follows: the hexadecyl trimethyl ammonium bromide neutralizing agent requires less inoculation volume and can breed fast by few bacterium; the result can be observed after the culture for 5 to 6 hours at the normal room temperature or 37 DEG C; the hexadecyl trimethyl ammonium bromide neutralizing agent can effectively neutralize the antibiotic and the sulfa drug in the sample and does not show false positive and false negative easily; and the hexadecyl trimethyl ammonium bromide neutralizing agent can damage the antimicrobial substance in the sample and ensure that the growth of the bacteria is not restrained.

Description

The hexadecane trimethyl ammonium bromide neutralizing agent

Technical field the present invention relates to a kind of hexadecane trimethyl ammonium bromide neutralizing agent, and its main component is that extractum carnis, peptone, sodium-chlor, agar powder, hexadecane trimethyl ammonium bromide are made through certain technology.Use for general sample and used in the patient specimen of microbiotic and sulfa drug and separation and Culture usefulness.Utilize culture experiment to differentiate the kind of bacterium, it is accurate, quick, easy that its key is.And microbionation separation and Culture quality can directly influence detected result.In often needing sample undertaken by different methods and separation and Culture, carry out the discriminating of bacterial species, this is the most frequently used means of present Microbiological Lab.Because the range of application of culture condition is limit at present, there is not specific for the substratum of using with separation and Culture in the general sample, there are institute's isolation medium under-nutrition, incubation time length, are prone to problems such as false positive or false negative.Along with the progress of biological study, the enrichment medium that bacterium is differentiated is studied also in constantly developing both at home and abroad.Biological worker wishes to have a kind of substratum that can overcome above-mentioned insufficient separation and Culture----hexadecane trimethyl ammonium bromide neutralizing agent always.

Background technology the object of the present invention is to provide and a kind ofly can overcome the existing insufficient hexadecane trimethyl ammonium bromide neutralizing agent of differential medium.Similar substratum with other is compared possesses following characteristics: required inoculum size is little, and a small amount of bacterium can breed rapidly, under room temperature usually or 37 ℃ cultivate and got final product observations in 5-6 hour; In can be effectively and microbiotic and sulfa drug in the sample, be prone to false positive and false negative; Can destroy the antibacterial substance that exists in the sample, bacterial growth is not suppressed.

Summary of the invention main points of the present invention are to select suitable component, and rationally are mixed, through heat, dissolve, technology such as mixing, cooling, packing, high-temperature sterilization forms a kind of hexadecane trimethyl ammonium bromide neutralizing agent.

The prescription following (component and weight percent % thereof) that the present invention selects: extractum carnis 3.0-4.0; Peptone 10.0-12.0; Sodium-chlor 0.5-0.6; Agar powder 10.-12.0; Hexadecane trimethyl ammonium bromide 0.3-0.5; Distilled water adds to 100ml.

Basal culture medium contains rich nutrient contents, be the nutrition in the substratum and support composition as extractum carnis, peptone, agar powder, similar substratum with other is compared possesses following characteristics: required inoculum size is little, a small amount of bacterium can breed rapidly, under room temperature usually or 37 ℃ cultivate and got final product observations in 5-6 hour; In can be effectively and microbiotic and sulfa drug in the sample, be prone to false positive and false negative; Can destroy the antibacterial substance that exists in the sample, bacterial growth is not suppressed.

Sodium-chlor is used to adjust osmotic pressure; Be computing time after the specimen inoculation, under common room temperature or 37 ℃ of cultivations, cultivate after 12 hours, promptly with the method for scoring culture transferring to the selective medium with the separation pathogenic bacterium.

The preparation method of product of the present invention is:

1), with extractum carnis, peptone, sodium-chlor,, hexadecane trimethyl ammonium bromide, distilled water place in the Erlenmeyer flask, mix and make liquid soak agar powder;

2), heating for dissolving, correct pH to 7.4 while hot, filter with flannelette, be sub-packed in test tube or the flask, be placed in the pressure-steam sterilization device, with 1.05kg30 minute;

3), be chilled to about 50 ℃ pour plate after the taking-up. after solidifying, sample,, refrigerate standby as no bacterial growth in 37 ℃ of cultivations 18 ~ 24 hours.

The present invention has the following advantages: (1) required inoculum size is little, and incubation time is short, and a small amount of bacterium can breed rapidly, under room temperature usually or 37 ℃ cultivate and got final product observations in 8-12 hour; (2) be not prone to false positive or false negative; (3) can destroy the microbiotic that exists in the patient specimen and the bacteriostatic action of sulfa drug.

The present invention is described in further detail below in conjunction with embodiment for embodiment:

Be configured according to following table institute column data (weight percent %) and described step thereof:

Prescription one:

Extractum carnis 3.0-4.0

Peptone 10.0-12.0

Sodium-chlor 0.5-0.6

Agar powder 10.0-12.0

Hexadecane trimethyl ammonium bromide 0.3-0.5

Distilled water adds to 100ml

Prescription two:

Extractum carnis 3.5-4.0

Peptone 11.0-12.0

Sodium-chlor 0.5-0.6

Agar powder 11.0-12.0

Hexadecane trimethyl ammonium bromide 0.4-0.5

Distilled water adds to 100ml

Prescription three:

Extractum carnis 3.0-3.5

Peptone 10.0-11.5.0

Sodium-chlor 0.5-0.55

Agar powder 10.-11.5

Hexadecane trimethyl ammonium bromide 0.3-0.4

Distilled water adds to 100ml

Claims (2)

1. hexadecane trimethyl ammonium bromide neutralizing agent, its main component are that extractum carnis, peptone, sodium-chlor, agar powder, hexadecane trimethyl ammonium bromide are made through certain technology; It is characterized in that having following prescription (component and weight percent % thereof): extractum carnis 3.0-4.0; Peptone 10.0-12.0; Sodium-chlor 0.5-0.6; Agar powder 10.-12.0; Hexadecane trimethyl ammonium bromide 0.3-0.5; Distilled water adds to 100ml.

2, the preparation method of the described hexadecane trimethyl ammonium bromide neutralizing agent of a kind of claim 1 is characterized in that having following step:

1), with extractum carnis, peptone, sodium-chlor,, hexadecane trimethyl ammonium bromide, distilled water place in the Erlenmeyer flask, mix and make liquid soak agar powder;

2), heating for dissolving, correct pH to 7.4 while hot, filter with flannelette, be sub-packed in test tube or the flask, be placed in the pressure-steam sterilization device, with 1.05kg30 minute;

3), be chilled to about 50 ℃ pour plate after the taking-up. after solidifying, sample in 37 ℃ of cultivations 18 ~ 24 hours, no bacterial growth refrigerates standby.