CN102980801A - Loading device for intervertebral disc mechanics performance test - Google Patents
Loading device for intervertebral disc mechanics performance test Download PDFInfo
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- CN102980801A CN102980801A CN2012105846544A CN201210584654A CN102980801A CN 102980801 A CN102980801 A CN 102980801A CN 2012105846544 A CN2012105846544 A CN 2012105846544A CN 201210584654 A CN201210584654 A CN 201210584654A CN 102980801 A CN102980801 A CN 102980801A
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Abstract
The invention relates to a loading device for an intervertebral disc mechanics performance test. The loading device is composed of an upper loading head, an angle adjusting sliding block, an observing chamber, a high-speed camera, a lower loading head, a cone and a fixed jaw, wherein guide blocks are arranged at the upper loading head; the angle adjusting sliding block comprises a middle half cylinder, a front adjusting plate and a rear baffle plate, and circular arc guide grooves are respectively arranged on the front adjusting plate and the rear baffle plate and are in sliding fit with the front and rear guide blocks of the upper loading head; the high-speed camera is positioned in the observing chamber; the fixed jaw adopts a three-jaw structure, and a worm wheel is arranged at the fixed jaw and is matched with a worm to form a speed reducing mechanism to drive the fixed jaw to rotate; the cone is fixedly arranged in the three jaw on the fixed jaw; and an intervertebral disc is arranged on the cone and is contacted with the lower surface of the toughened glass of the lower loading head. The loading device has the advantages of simple structure and easiness in operation, can provide axial compression, contortion and lateral bending loading, has a better variable regulating function, can be used for engineered tissue culture and performance test of different segments of intervertebral discs of cervical or lumbar spines, and has a larger economic practicality.
Description
Technical field
The present invention relates to cell cultivation, field of tissue engineering technology, particularly a kind of charger of interverbebral disc Mechanics Performance Testing.
Background technology
Along with the development of computer technology, modern optical electronic technology and Digital Image Processing level, Digital Image Correlation Method becomes a kind of important method of testing in Experimental Mechanics field day by day.The present invention adopts Digital Image Correlation Method that a kind of new method of carrying out the interverbebral disc Mechanics Performance Testing is provided for improving and overcoming the problem that obviously exists in the existing research of interverbebral disc mechanical test and fail the comprehensive and reasonable solution.Be included in observe surface gear glass embed dark nano particle spot processed with impact, the interverbebral disc surface of avoiding boundary effect so that obtain clear accurately image and in loading procedure with making test pattern more clear in the stand under load interverbebral disc immersion physiological saline, more be conducive to the measurement of interverbebral disc mechanical property.
Interverbebral disc firmly connects adjacent vertebral to keep the arrangement of canalis spinalis between adjacent vertebral, and thickness accounts for 1/4th of the above backbone total length of rumpbone.Interverbebral disc is comprised of fibrous ring, nucleus pulposus and cartilaginous opereulum three parts.The main bearing tension of peripheral fibres ring, nucleus pulposus mainly is stressed.Compare the independent stand under load of each tissue, they make the as a whole more complicated load of bearing, as crooked, reverse etc., this also further illustrates the importance of complete institutional framework.Cartilaginous opereulum plays vital effect for the growth and development of nucleus pulposus cell, is protecting simultaneously contiguous centrum, prevents thrusting of spinous process.
Along with the quickening of people's rhythm of life, intervertebral disc degeneration and other spondylodynias have caused the mankind's life and economy and have had a strong impact on.Deepening continuously of the foundation, particularly cartilage tissue engineered research of external nucleus pulposus and fibrous ring histocyte culture technique in recent years is for the morphosis of regression interverbebral disc and the holomorphosis reparation of physiological function have brought hope.
Organizational project refers to principle and method with engineering science and life science, and preparation tissue and organ substitute to recover keeping or the improve function of human tissue organ, are rapid far-reaching biomedical engineering applications of development.At present, the tissue engineering skin product formally advances people's clinical practice, and organizational project bone, cartilage, skin and the nerve fiber of cultivation carried out experiment in the body.But, at present the organizational project distance be widely used in clinical, become social economy's new growth point and also have quite long road to walk.The organizational project bio-reactor is environment similar in one of external achievements and body, accelerate the system of cultured cell, tissue and even organ, can be widely used in substituting, make up, keeping or strengthen its function of organization such as the organ defect patient, and the field such as bio-pharmaceuticals.By the various environmental quality mechanics under the body physiological condition of research cell tissue growth, biochemical environment etc., how study mechanics under the external environment, biochemical factors affects dimensional culture and the functionalization process of cell, especially significant for the bionical charger of more rational design in the mechanics problem of soma.
Powell, Courtney A. etc. has designed a kind of bio-reactor that skeletal muscle tissue is cultivated that can be used in, one section on skeletal muscle tissue support is fixing, one section links to each other with stepper motor, provides constant or tension and compression stress periodically by stepper motor, and the suffered tension and compression stress of support detects by sensor, organize culture chamber not rotate, referring to Mechanical stimulation improvestissue-engineeredhuman skeletal muscle, Am J Physiol, 2002; 282(5): 1557-1565." method and bio-reactor that a kind of low-frequency high-amplitude overlapped high-frequency loads by a narrow margin " adopts two series voltages pottery shifter as driving the source in the Chinese NO.200710303886.7 patent, can load the stack of cartilage double frequency.Many scholars have done a large amount of research for the centrum motion segment, referring to: the dissection of spinal motion and biomechanical basis, Chinese Journal of Physical Medicine and Rehabilitation the 5th phase of the 26th volume May in 2004,308-310, article the relation of spinal motion and stand under load, and the correlation of different loads and each institutional framework.Document: " biomechanics under the different operating modes of applying three-dimensional finite element model research cervical vertebra changes ", clinical orthopaedics magazine .2003Dec, 6 (4) .294-296, the mechanical environment of cervical vertebra that adopted analysis of finite element method emulation.Document: " biomechanics of artificial lumbar disc replacement and mid-term clinical curative effect analysis ", Chinese journal of orthopedics the 27th volume the 5th phase .374-377 May in 2007, after having analyzed normal cervical vertebra and displacement artificial cervical dish in conjunction with clinical effectiveness, mechanics link and structural variation between centrum and the interverbebral disc.
In sum, analysis and research are organized in the mechanics environment, and its true biomechanics environment of design simulation is most important for the constructing function histoorgan.So far, do not reported also that a kind of interverbebral disc of can simulating was in the loading system of the complicated mechanical environment of body, simultaneously to lateral bending and reverse etc. mechanical load to interverbebral disc mechanical state carry out real-time monitored, and gather image in order to determine the strain field distribution of interverbebral disc, explore the mechanical state of interverbebral disc, analyse scientifically for large software simultaneously basic data is provided.
Summary of the invention
The objective of the invention is for above-mentioned existing problems, a kind of charger of interverbebral disc Mechanics Performance Testing is provided, this device has bending, reverse and axially loading and the test mechanics status system of dynamic compression function, several different Mechanical loadings can be provided simultaneously, realize the coupling of different mechanical conditions, for tissue growth provides the biomechanics environment that is similar at body, also can carry out various Mechanics Performance Testings simultaneously.
Technical scheme of the present invention:
A kind of charger of interverbebral disc Mechanics Performance Testing, formed by upper loading head, angular adjustment slide block, sight chamber, high-speed camera head, lower loading head, centrum and fixed claw, upper loading head is the rectangular block that the bottom surface is the face of cylinder, two each sides of upper loading head are respectively equipped with guide block, be provided with foresight in the middle of the front of upper loading head, upper loading head is fixed in the lower surface of mechanical loading unit; The angular adjustment slide block is I shape semicircle ladder post, semicolumn in the middle of comprising, front adjustable plate and backboard, the upper face of cylinder of middle semicolumn and the face of cylinder, the end of upper loading head form and are slidingly matched, the two sides of upper loading head and I shape semicircle ladder post two medial surfaces are clearance fit, the lower surface of middle semicolumn is provided with the square boss, be respectively equipped with the circular arc guide groove on front adjustable plate and the backboard and form with the front and back guide block of upper loading head and be slidingly matched, the front surface circular arc line of front adjustable plate is provided with scale, the front surface of front adjustable plate is provided with two screws and fixes by lock-screw and upper loading head, and the side of front adjustable plate and backboard is respectively equipped with four screws and fixes by gib screw and sight chamber side plate; Sight chamber is the hollow square molding box body that is comprised of four blocks of plates, and the angular adjustment slide block is embedded between the sight chamber biside plate by the square boss of middle semicolumn lower surface, and four plate bottom surfaces of sight chamber are cascaded surface and whole formation square boss; The high-speed camera head is positioned at sight chamber and is fixed in the bottom surface of angular adjustment slide block boss; Lower loading head is rectangular box, and its length and width size is identical with sight chamber, and the upper surface center of lower loading head is provided with the square groove and inlays with the square boss of sight chamber bottom and is fixed, and in the lower loading head framework tempered glass is housed; Fixed claw is the three-pawl type structure, and fixed claw is provided with turbine and cooperates with worm screw and consists of reducing gear and drive the fixed claw rotation, and centrum is fixed in the three-jaw above the fixed claw, and interverbebral disc places on the centrum and with the tempered glass lower surface of lower loading head and contacts.
The principle of work of this charger:
By regulating load angle, the stress of simulation human intervertebral disc when the various angular bend of backbone by the associated change of cameras view record stand under load body surface generation, utilized the present computer technology analysis again.The high-speed camera head is housed, all the time perpendicular to being loaded body surface observation in the sight chamber.This device can pass through the adjusting angle adjusting slider in ± 15 ° of scopes, realize crooked value-added tax function; By the rotation fixed claw, can realize reversing value-added tax function; By add the moving device of rotational oscillation at upper loading head, then can realize dynamic loading function.
Loading system of the present invention with the advantage that existing mechanical loading unit is compared is:
1) this charger axial compression is provided, reverse with lateral bending in any one, two or three multi-form actings in conjunction, realize the coupling of mechanical load, more near true physiological mechanics state in the body;
2) this charger has preferably variable regulatory function, and the load of different sizes can be provided, and can design dependent variable, independent variable carries out the biology control test, and the comparative analysis different loads is on the impact of tissue growth, the validity that demo plant loads;
3) loading force of this charger is a kind of concrete but infinite enforcement power, not only can be used for testing each stage interverbebral disc, centrum engineering tissue biology and the test apparatus of mechanical property, can also cultivate as cervical vertebra or lumbar vertebrae different segment interverbebral disc engineering tissue, larger economic and practical is arranged;
4) can utilize observation and analysis testee loaded state under the real-time load of Digital image technology.
Description of drawings
Fig. 1 is this charger structural representation.
Fig. 2 is upper loading head structure enlarged diagram.
Fig. 3 is angular adjustment slide block structure enlarged diagram.
Fig. 4 is this charger stereographic map.
Among the figure: 1. go up 5. times loading heads of loading head 2. angular adjustment slide block 3. sight chambers, 4. high-speed camera heads, 6. centrums, 7. fixed claws, 8. guide blocks, 9. middle semicolumn 10. front adjustable plate 11. backboards 12. guide grooves 13. lock-screws 14. gib screws 15. interverbebral discs 16. turbines 17. worm screws 18. bases 19. scales 20. foresights
Embodiment
Below in conjunction with accompanying drawing loading system example of the present invention is described in detail.
Embodiment:
A kind of charger of interverbebral disc Mechanics Performance Testing, such as Fig. 1-shown in Figure 4, formed by upper loading head 1, angular adjustment slide block 2, sight chamber 3, high-speed camera head 4, lower loading head 5, centrum 6 and fixed claw 7, upper loading head 1 is the rectangular block on the face of cylinder for the bottom surface, two each sides of upper loading head 1 are respectively equipped with guide block 8, be provided with foresight 20 in the middle of the front of upper loading head 1, upper loading head 1 is fixed in the lower surface of mechanical loading unit; Angular adjustment slide block 2 is I shape semicircle ladder post, semicolumn 9 in the middle of comprising, front adjustable plate 10 and backboard 11, the upper face of cylinder of middle semicolumn 9 and the face of cylinder, the end of upper loading head 1 form and are slidingly matched, the two sides of upper loading head and I shape semicircle ladder post two medial surfaces are clearance fit, the lower surface of middle semicolumn 9 is provided with the square boss, be respectively equipped with circular arc guide groove 12 on front adjustable plate 10 and the backboard 11 and form with the front and back guide block 8 of upper loading head 1 and be slidingly matched, the front surface circular arc line of front adjustable plate 10 is provided with scale 19, the front surface of front adjustable plate 10 is provided with two screws and fixes by lock-screw 13 and upper loading head 1, and the side of front adjustable plate 10 and backboard 11 is respectively equipped with four screws and fixes by gib screw 14 and sight chamber 3 side plates; Sight chamber 3 is the hollow square molding box body that is comprised of four blocks of plates, and angular adjustment slide block 2 is embedded between sight chamber 3 biside plates by the square boss of middle semicolumn 9 lower surfaces, and four plate bottom surfaces of sight chamber 3 are cascaded surface and whole formation square boss; High-speed camera head 4 is positioned at sight chamber 3 and is fixed in the bottom surface of angular adjustment slide block 2 boss; Lower loading head 5 is rectangular box, and its length and width size is identical with sight chamber 3, and the upper surface center of lower loading head 5 is provided with the square groove and inlays with the square boss of sight chamber 3 bottoms and is fixed, and in the framework of lower loading head 5 tempered glass is housed; Fixed claw 7 is the three-pawl type structure, fixed claw 7 is provided with turbine 16 and cooperates the formation reducing gear to drive fixed claw 7 rotations with worm screw 17, centrum 6 is fixed in the three-jaw above the fixed claw 7, and interverbebral disc 15 places on the centrum 6 and contacts with tempered glass lower surface in lower loading head 5 frameworks.
The working routine of this charger:
1) spot processed
Get the centrum test sample book with interverbebral disc, sample is fixed with fixed claw, block clear glass at the interverbebral disc sightingpiston in the fixation procedure, the cartilage surface of observing at need embeds dark nano particle spot processed, and the interverbebral disc sightingpiston immerses in the physiological saline and can make test pattern more clear.In the interverbebral disc loading procedure, cartilage observation obtains clear and spotted image, adopts Digital Image Correlation Method to carry out the analysis of interverbebral disc mechanical property.
2) adjusting load device parameters
This is a kind of Observable charger of simulating mechanical environment of vertebral body, comprises lateral bending and reverses function.At first determine research method according to the object of studying, determine proper parameter, such as the angle of regulating, the size of the power of loading.The angle of regulating comprises angle, the anglec of rotation of fixed claw and the size of loading force between angular adjustment slide block and the upper loading blocks.
The below specifies respectively how to regulate above said parameters:
1) angle of bend
At first, unclamp lock-screw, the adjusting angle adjusting slider contrasts institute's target scale on it, is transferred to required angle, and concrete range of adjustment is ± 15 °, accurately be ± 0.05-0.1.After being transferred to required angle, lock-screw, according to the size of the power that will load, the locking degree of set screw is to reach the effect of self-protection; In case loading force is excessive, slide block will begin to slide in guide groove, thereby loading procedure stops, and plays holding function.
2) the fixed claw anglec of rotation
Utilize turbine worm reducer structure realization fixed claw rotation and have auto-lock function.This regulates and minute dynamically to regulate and two kinds of Static adjusts, and Static adjust utilizes worm screw rotation fixed claw before being test, because interverbebral disc upper end and lower loading head are without relative motion, so will produce definite torsional moment; Dynamic adjustments is to utilize worm screw to regulate fixed claw in loading, thereby produces the dynamic test torsional moment.
Claims (1)
1. the charger of an interverbebral disc Mechanics Performance Testing, it is characterized in that: formed by upper loading head, angular adjustment slide block, sight chamber, high-speed camera head, lower loading head, centrum and fixed claw, upper loading head is the rectangular block that the bottom surface is the face of cylinder, two each sides of upper loading head are respectively equipped with guide block, be provided with foresight in the middle of the front of upper loading head, upper loading head is fixed in the lower surface of mechanical loading unit; The angular adjustment slide block is I shape semicircle ladder post, semicolumn in the middle of comprising, front adjustable plate and backboard, the upper face of cylinder of middle semicolumn and the face of cylinder, the end of upper loading head form and are slidingly matched, the two sides of upper loading head and I shape semicircle ladder post two medial surfaces are clearance fit, the lower surface of middle semicolumn is provided with the square boss, be respectively equipped with the circular arc guide groove on front adjustable plate and the backboard and form with the front and back guide block of upper loading head and be slidingly matched, the front surface circular arc line of front adjustable plate is provided with scale, the front surface of front adjustable plate is provided with two screws and fixes by lock-screw and upper loading head, and the side of front adjustable plate and backboard is respectively equipped with four screws and fixes by gib screw and sight chamber side plate; Sight chamber is the hollow square molding box body that is comprised of four blocks of plates, and the angular adjustment slide block is embedded between the sight chamber biside plate by the square boss of middle semicolumn lower surface, and four plate bottom surfaces of sight chamber are cascaded surface and whole formation square boss; The high-speed camera head is positioned at sight chamber and is fixed in the bottom surface of angular adjustment slide block boss; Lower loading head is rectangular box, and its length and width size is identical with sight chamber, and the upper surface center of lower loading head is provided with the square groove and inlays with the square boss of sight chamber bottom and is fixed, and in the framework of lower loading head tempered glass is housed; Fixed claw is the three-pawl type structure, and fixed claw is provided with turbine and cooperates with worm screw and consists of reducing gear and drive the fixed claw rotation, and centrum is fixed in the three-jaw above the fixed claw, and interverbebral disc places on the centrum and with the lower surface of lower loading head tempered glass and contacts.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210584654.4A CN102980801B (en) | 2012-12-27 | 2012-12-27 | Loading device for intervertebral disc mechanics performance test |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210584654.4A CN102980801B (en) | 2012-12-27 | 2012-12-27 | Loading device for intervertebral disc mechanics performance test |
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| CN102980801A true CN102980801A (en) | 2013-03-20 |
| CN102980801B CN102980801B (en) | 2015-07-01 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105628601A (en) * | 2015-12-21 | 2016-06-01 | 天津理工大学 | Rolling load application control device for relevant cartilage experiment based on digital image |
| CN105699224A (en) * | 2016-04-08 | 2016-06-22 | 浙江大学城市学院 | Lumbar fatigue testing machine |
| CN106404536A (en) * | 2016-10-24 | 2017-02-15 | 张红 | Multi-angle spinal biomechanical loading measurement device |
| CN106644539A (en) * | 2016-10-10 | 2017-05-10 | 董亚伦 | Spinal biomechanics measurement multi-angle loading device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102147990A (en) * | 2011-03-08 | 2011-08-10 | 天津理工大学 | Loading device for simulating mechanical environment of vertebral body |
| JP4756113B2 (en) * | 2005-12-21 | 2011-08-24 | 国立大学法人 新潟大学 | Lumbar mobility measurement system |
| CN202497132U (en) * | 2011-12-31 | 2012-10-24 | 徐宏光 | Animal intervertebral disc body assistor |
-
2012
- 2012-12-27 CN CN201210584654.4A patent/CN102980801B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4756113B2 (en) * | 2005-12-21 | 2011-08-24 | 国立大学法人 新潟大学 | Lumbar mobility measurement system |
| CN102147990A (en) * | 2011-03-08 | 2011-08-10 | 天津理工大学 | Loading device for simulating mechanical environment of vertebral body |
| CN202497132U (en) * | 2011-12-31 | 2012-10-24 | 徐宏光 | Animal intervertebral disc body assistor |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105628601A (en) * | 2015-12-21 | 2016-06-01 | 天津理工大学 | Rolling load application control device for relevant cartilage experiment based on digital image |
| CN105628601B (en) * | 2015-12-21 | 2018-06-08 | 天津理工大学 | A kind of rolling load for cartilage digital picture related experiment applies control device |
| CN105699224A (en) * | 2016-04-08 | 2016-06-22 | 浙江大学城市学院 | Lumbar fatigue testing machine |
| CN106644539A (en) * | 2016-10-10 | 2017-05-10 | 董亚伦 | Spinal biomechanics measurement multi-angle loading device |
| CN106644539B (en) * | 2016-10-10 | 2018-11-30 | 陈刚 | Spinal biomechanics measure multi-angle loading device |
| CN106404536A (en) * | 2016-10-24 | 2017-02-15 | 张红 | Multi-angle spinal biomechanical loading measurement device |
| CN106404536B (en) * | 2016-10-24 | 2019-01-08 | 浙江瑞邦智能装备股份有限公司 | Spinal biomechanics multi-angle loads measuring device |
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| CN102980801B (en) | 2015-07-01 |
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