CN102972350A - Method for predicting fertility of ganders - Google Patents

Method for predicting fertility of ganders Download PDF

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Publication number
CN102972350A
CN102972350A CN2012105695084A CN201210569508A CN102972350A CN 102972350 A CN102972350 A CN 102972350A CN 2012105695084 A CN2012105695084 A CN 2012105695084A CN 201210569508 A CN201210569508 A CN 201210569508A CN 102972350 A CN102972350 A CN 102972350A
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massage
reaction
gander
sperm
ganders
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CN102972350B (en
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刘胜军
张莹
李景春
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Heilongjiang Bayi Agricultural University
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Heilongjiang Bayi Agricultural University
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Abstract

The invention relates to a method for predicting the fertility of ganders. The method comprises the steps as follows: 1, determining response types according to the massaging stimulation response degree and speed, and carrying out objective scoring on the ganders; 2, in the beginning of a breeding season, stimulating the genital organs of the ganders by adopting a back and belly type massaging method, recording the time from massaging stimulation to ejaculation reflection at the same time, and collecting seminal fluids; 3, determining the individuals with the massaging stimulation scores of 2 and 3 according to different responses of the individuals on the massaging stimulation process as the qualified ganders; and 4, measuring the seminal fluid quantity, the seminal fluid concentration and the sperm activity of the ganders according to the seminal fluid quality. According to the method, the ganders are early selected according to stronger positive correlation of the early massaging stimulation response and the seminal fluid quality of the ganders in the whole breeding season, so that the gander selection accuracy is improved and the breeding efficiency is improved.

Description

A kind of kind of method of gander fertility of predicting
One, technical field
What the present invention relates to is the method for distinguishing male poultry fecundity, and what be specifically related to is a kind of kind of method of gander fertility of predicting.
Two, background technology
Semen quality is widely different between the goose Different Individual, is difficult in the production practices on the goose profile the gander fertility be distinguished.Mainly select by size, color and the semen quality situation of observing the gander reproductive organs in the production practices.There is certain defective in this method, and goose semen quality Character instability begins the good individuality of semen quality in other words in mating season, might not good performance still be arranged in breeding mid-term and later stage.
Three, summary of the invention
The method that the purpose of this invention is to provide prediction kind of gander fertility, the method of this prediction kind gander fertility is used for dealing with problems: in incipient stage mating season, the gander of only selecting by size, color and the semen quality situation of gander reproductive organs, it is in intermediary and later stages mating season performance and unstable, i.e. the breeding at gander initial stage mating season performance can not represent this individuality good reproductive performance whole mating season.This method is with solving the not accurate enough problem of present selection kind gander fertility method.
The technical solution used in the present invention is: the method for this prediction kind gander fertility is as follows.
One, determines reaction type according to massage irritant reaction degree and speed, gander is carried out objective scoring.Reaction type is four kinds, and the first type represents with 0, and its massage reaction is: do not have reaction or reaction more than 60 seconds slowly to massaging to stimulate, do not ejaculate; The second type with 1 representative, its massage reaction is: fast to the massage irritant reaction, and in 60 seconds, just produce reaction, but do not have ejaculation; The third type with 2 representatives, its massage reaction is: slow to the massage irritant reaction, and need massage to stimulate and just respond more than 60 seconds, but ejaculation; Four types represents with 3, and its massage reaction is: fast to the massage irritant reaction, the massage stimulation is less than 60 seconds and just responds, and ejaculation;
Two, when begin mating season, adopt the sexual organ of back of the body abdomen formula massage stimulation gander, simultaneously recording start massage stimulated to the generation ejaculation reflex time, and collected seminal fluid;
Three, different to the massage irritant reaction according to individuality, stimulate scoring at 2 minutes and 3 minutes individualities to massage, determine it is qualified gander;
Four, according to the height of semen quality, measure quantity, seminal concentration and the sperm viability of goose seminal fluid, measurement result showed at the beginning of mating season, fast and individuality ejaculation often has higher seminal fluid quantity and sperm concentration to the massage irritant reaction, and namely goose is to degree and goose whole mating season of sperm volume and the average seminal concentration high-positive correlation of massage reaction; The standard that semen quality is selected is normally sperm ratio alive of every gander ejaculum amount * sperm concentration * form.Sperm volume unit is milliliter, and sperm concentration unit is * 10 6ML-1, form normally sperm ratio alive adopts %, measures the seminal fluid volume with smart glass of collection.
The present invention in the standard of screening kind of gander is: begin in mating season, according to the massage reaction, selecting massage stimulates score at the individuality more than 2, makes a choice according to semen quality situation (standard of semen quality selection is normally sperm ratio alive of every gander ejaculum amount * sperm concentration * form) again.
Beneficial effect:
1, the present invention according to early stage massage irritant reaction and whole mating season the goose semen quality have stronger positive correlation, gander is made early stage selection, improved the accuracy that gander is selected, improved reproductive efficiency.
2, the present invention is applicable to the artificial insemination work of goose.Select relatively with the simple profile of traditional basis and semen quality.This option program has the accuracy rate height, successful, definite characteristics.
Four, embodiment
Embodiment 1:
One, determine reaction type according to massage irritant reaction degree and speed, carry out objective scoring, reaction type is four kinds, and the first type represents with 0, and its massage is reacted and is: massage is stimulated not to be had reaction or reacts slow more than 60 seconds, do not ejaculate; The second type with 1 representative, its massage reaction is: fast to the massage irritant reaction, and in 60 seconds, just produce reaction, but do not have ejaculation; The third type with 2 representatives, its massage reaction is: slow to the massage irritant reaction, and need massage to stimulate and just respond more than 60 seconds, but ejaculation; Four types represents with 3, and its massage reaction is: fast to the massage irritant reaction, the massage stimulation is less than 60 seconds and just responds, and ejaculation;
Two, when begin mating season, the sexual organ that adopt back of the body abdomen formula massage to stimulate gander 1, simultaneously recording start massage stimulates to producing the ejaculation reflex time, this gander 1 produces reaction when stimulating 58 seconds, and ejaculation is arranged, and collect seminal fluid, measuring the seminal fluid volume with the smart cup of collection is 0.41 milliliter.
Three, be 3 minutes to the massage stimulation scoring of gander 1, therefore determine it is qualified gander;
Four, the seminal fluid of collecting according to step 2, the height of analysis semen quality in rear 30 minutes of collection, is studied ejaculatory amount, pH value, seminal concentration, sperm morphology and gander semen quality parameter to following parameter.After the dyeing of Yihong nigrosine, carry out the morphology evaluation (Bakst and Ceci, 1997) of spermoblast; Sperm concentration can be measured with blood cell counting plate, and motion state can be estimated with 400 times of amplifications of hangdrop method.
Gander semen quality parameter=every gander ejaculum amount (milliliter) * sperm concentration (* 10 6ML-1) * the form sperm ratio (%) of living normally.
After measured, the quantity of gander 1 seminal fluid is that 0.41 ml, seminal concentration are 570 * 10 6Ml -1, sperm viability 54.2%, the form sperm 42.7% of living normally, according to the semen quality selection criteria: gander ejaculum amount * sperm concentration * form sperm ratio of living normally, wherein sperm volume unit be milliliter, sperm concentration unit is * 10 6ML-1, form normally sperm ratio alive adopts %, and calculate and learn that this gander 1 semen quality parameter is 98.1, be the strong gander of fertility, be qualified gander, should select.
Embodiment 2:
One, determine reaction type according to massage irritant reaction degree and speed, carry out objective scoring, reaction type is four kinds, and the first type represents with 0, and its massage is reacted and is: massage is stimulated not to be had reaction or reacts slow more than 60 seconds, do not ejaculate; The second type with 1 representative, its massage reaction is: fast to the massage irritant reaction, and in 60 seconds, just produce reaction, but do not have ejaculation; The third type with 2 representatives, its massage reaction is: slow to the massage irritant reaction, and need massage to stimulate and just respond more than 60 seconds, but ejaculation; Four types with 3 representatives, its massage reaction is: fast for reaction to the massage stimulation, the massage stimulation is less than 60 seconds and just responds, and ejaculation;
Two, when begin mating season, the sexual organ that adopt back of the body abdomen formula massage to stimulate gander 2, simultaneously recording start massage stimulates to producing the ejaculation reflex time, this gander 2 produces reaction when stimulating 61 seconds, but ejaculation is arranged, collect seminal fluid, measuring the seminal fluid volume with the smart cup of collection is 0.31 milliliter.
Three, be 2 minutes to the massage stimulation scoring of gander 2, therefore determine it is qualified gander;
Four, the seminal fluid of collecting according to step 2, the height of analysis semen quality in rear 30 minutes of collection, is studied ejaculatory amount, pH value, seminal concentration, sperm morphology and gander semen quality parameter to following parameter.After the dyeing of Yihong nigrosine, carry out the morphology evaluation (Bakst and Ceci, 1997) of spermoblast; Sperm concentration can be measured with blood cell counting plate, and motion state can be estimated with 400 times of amplifications of hangdrop method.
Gander semen quality parameter=every gander ejaculum amount (milliliter) * sperm concentration (* 10 6ML -1) * form is sperm ratio (%) alive normally.
After measured, the quantity of gander 2 seminal fluid is that 0.31 milliliter, seminal concentration are 327 * 10 6Ml -1With sperm viability 51.8%, form is sperm 35.6% alive normally, and the standard of selecting according to semen quality is normally sperm ratio alive of every gander ejaculum amount * sperm concentration * form, and wherein sperm volume unit is milliliter, and sperm concentration unit is * 106mL -1, form normally sperm ratio alive adopts %, and thus, these gander 2 semen quality parameters are 36.4, are the stronger ganders of fertility, are qualified gander, should select.Gander semen quality parameter was greater than 30 o'clock generally speaking, and this gander fertility is stronger.
Above-described embodiment 1 and embodiment 2 carry out under the state of maintaining secrecy, after above-mentioned gander made one's options, in early stage, mid-term of gander mating season, the tracking and measuring that the later stage has been carried out omnidistance semen quality, observed result is: in whole mating season, gander 1,2 has preferably semen quality all the time, but gander 1 is better than gander 2 all the time, illustrates that the method accuracy rate of prediction kind gander fertility provided by the invention is high, is very feasible.

Claims (1)

1. predict kind of the method for gander fertility for one kind, it is characterized in that: the method for this prediction kind gander fertility is as follows:
One, determine reaction type according to massage irritant reaction degree and speed, carry out objective scoring, reaction type is four kinds, and the first type represents with 0, and its massage is reacted and is: massage is stimulated not to be had reaction or reacts slow more than 60 seconds, do not ejaculate; The second type with 1 representative, its massage reaction is: fast to the massage irritant reaction, and in 60 seconds, just produce reaction, but do not have ejaculation; The third type with 2 representatives, its massage reaction is: slow to the massage irritant reaction, and need massage to stimulate and just respond more than 60 seconds, but ejaculation; Four types represents with 3, and its massage reaction is: fast to the massage irritant reaction, the massage stimulation is less than 60 seconds and just responds, and ejaculation;
Two, when begin mating season, adopt the sexual organ of back of the body abdomen formula massage stimulation gander, simultaneously recording start massage stimulated to the generation ejaculation reflex time, and collected seminal fluid;
Three, different to the massage irritant reaction according to individuality, stimulate scoring at 2 minutes and 3 minutes individualities to massage, determine it is qualified gander;
Four, according to the height of semen quality, measure quantity, seminal concentration and the sperm viability of goose seminal fluid, measurement result showed at the beginning of mating season, fast and individuality ejaculation often has higher seminal fluid quantity and sperm concentration to the massage irritant reaction, namely massages the extent of reaction and goose whole mating season of sperm volume and average seminal concentration positive correlation; The standard that semen quality is selected is normally sperm ratio alive of every gander ejaculum amount * sperm concentration * form, and sperm volume unit is milliliter, and sperm concentration unit is * 10 6ML -1, form normally sperm ratio alive adopts % to represent, measures the seminal fluid volume with smart glass of collection.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103503830A (en) * 2013-09-26 2014-01-15 赤峰巴林左旗福山畜禽有限责任公司 Method for improving reproductive performance of gooses
CN108315436A (en) * 2018-03-23 2018-07-24 黑龙江八农垦大学 A method of being applied to goose paternity test
CN112586438A (en) * 2020-11-04 2021-04-02 中国农业大学 Method for evaluating fertility of sires

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1017323A1 (en) * 1982-03-12 1983-05-15 Всесоюзный Ордена Трудового Красного Знамени Научно-Исследовательский И Технологический Институт Птицеводства Method of obtaining sperm from poultry males
FR2574281A1 (en) * 1984-12-11 1986-06-13 Ardailhon Miramon Jean D Seminal liquid collector for male palmipeds (web-footed birds)
SU1570684A1 (en) * 1988-05-10 1990-06-15 Чувашский сельскохозяйственный институт Method of selecting goose-ganders by sperm quality
WO2008117026A1 (en) * 2007-03-23 2008-10-02 The Royal Veterinary College Method for enhancing sperm survival
CN102369907A (en) * 2011-09-17 2012-03-14 兰州宏庆科技有限公司 Method for breeding special wild chicken through artificial fertilization

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1017323A1 (en) * 1982-03-12 1983-05-15 Всесоюзный Ордена Трудового Красного Знамени Научно-Исследовательский И Технологический Институт Птицеводства Method of obtaining sperm from poultry males
FR2574281A1 (en) * 1984-12-11 1986-06-13 Ardailhon Miramon Jean D Seminal liquid collector for male palmipeds (web-footed birds)
SU1570684A1 (en) * 1988-05-10 1990-06-15 Чувашский сельскохозяйственный институт Method of selecting goose-ganders by sperm quality
WO2008117026A1 (en) * 2007-03-23 2008-10-02 The Royal Veterinary College Method for enhancing sperm survival
CN102369907A (en) * 2011-09-17 2012-03-14 兰州宏庆科技有限公司 Method for breeding special wild chicken through artificial fertilization

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
屈年录: "鹅选种和繁育的基本方法", 《养殖技术顾问》, no. 7, 5 July 2008 (2008-07-05), pages 35 *
曾凡同 等: "《养鹅全书》", 31 January 2002, article "鹅的人工授精", pages: 68 - 73 *
梁裕利 等: "德国牧羊犬雄犬繁殖能力的测定", 《畜牧兽医科技》, no. 2, 2 July 1987 (1987-07-02), pages 15 - 16 *
齐庆凡: "掌握鹅繁殖技术是提高繁殖力的保障", 《农村养殖技术》, no. 10, 15 May 2010 (2010-05-15), pages 16 - 17 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103503830A (en) * 2013-09-26 2014-01-15 赤峰巴林左旗福山畜禽有限责任公司 Method for improving reproductive performance of gooses
CN103503830B (en) * 2013-09-26 2015-08-12 赤峰巴林左旗福山畜禽有限责任公司 Improve the method for supporting goose Reproduction performance
CN108315436A (en) * 2018-03-23 2018-07-24 黑龙江八农垦大学 A method of being applied to goose paternity test
CN108315436B (en) * 2018-03-23 2023-01-31 黑龙江八一农垦大学 Method applied to goose paternity test
CN112586438A (en) * 2020-11-04 2021-04-02 中国农业大学 Method for evaluating fertility of sires

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