CN102972123B - Rapid adonis amurensis seed after-ripening method - Google Patents
Rapid adonis amurensis seed after-ripening method Download PDFInfo
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- CN102972123B CN102972123B CN201210502783.4A CN201210502783A CN102972123B CN 102972123 B CN102972123 B CN 102972123B CN 201210502783 A CN201210502783 A CN 201210502783A CN 102972123 B CN102972123 B CN 102972123B
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Abstract
The invention relates to a seed breeding method, and particularly relates to a rapid adonis amurensis seed after-ripening method. The after-ripening method comprise the following steps of: drying adonis amurensis aggregate fruit balls, removing seed coats of the aggregate fruit balls, and immersing the aggregate fruit balls in a mixed solution of 2.50-3.00 mg/L 2,4-dichlorphenoxyacetic acid and 0.60-0.70 mg/L 6-benzylaminopurine for 48h; and uniformly mixing seeds with river sand, and carrying out temperature changing treatment on the mixture in a wooden tank indoors for 88-102 days, wherein the wooden tank has a good ventilation property and is covered with a plastic film, thus finally developing the seeds into mature cotyledon embryo seeds. The temperature changing amplitude and time of the temperature-changing treatment are that the daytime temperature is 20+/-2 DEG C and the treatment lasts for 12h, and the night temperature is 10+/-2 DEG C and the treatment lasts for 12h; and the mixing ratio of the seeds to the river sand is 1:3. Based on the treatment by the method, the adonis amurensis seeds develop from globular embryo into heart-shaped embryo and finally develop into the mature cotyledon embryo seeds. Low-temperature treatment breaks cotyledon embryo dormancy to achieve seed germination; and the method has a great significance to artificial large-scale plantation of high-quality adonis amurensis seedlings.
Description
technical field
The present invention relates to a kind of method for cultivating seeds, i.e. a kind of quick after-ripening method of adonis amurensis seed.
Background technology
In the prior art, adonis amurensis (
adonis amurensisregel et Radde) be Ranunculaceae adonis amurensis Pittosporum herbaceos perennial.The high 5-30cm of plant, grows thickly, and spend and be singly born in stem top, flower foresythia, glossy, the florescence 4-5 month, etaerio of achenes is spherical, the fruit phase 5-6 month.Adonis amurensis, for top snow flowering plant in early spring, can be used for afforestation in early spring.During adonis amurensis fruit maturation, the embryo in seed is in the globular embryo stage, and planting seed is not sprouted then, needs the nearly 2 years a small amount of germinating growths of ability, and exploitation are extremely restricted.
Summary of the invention
The object of the invention is to provide for above-mentioned deficiency a kind of quick after-ripening method of adonis amurensis seed of condition of the morphogenesis of embryo fast.
Technical solution of the present invention is: a kind of quick after-ripening method of adonis amurensis seed, its step is as follows: after adonis amurensis aggregate fruit ball is dried, slough after kind of skin, through 2,4-dichlorphenoxyacetic acid, 2.50～3.00 mgL
-1with 6-benzyl aminopurine 0.60～0.70 mgL
-1in mixed solution, soak after 48 h, seed and river sand are mixed in the trogue that all packs afterwards good permeability into, covered with plastic film is placed in the indoor alternating temperature carrying out 88～109 d days and processes, and is finally developed to ripe cotyledonary embryos seed.
Alternating temperature amplitude and time that alternating temperature is processed are: day temperature is 20 ± 2 ℃, processes 12 h; Nocturnal temperature is 10 ± 2 ℃, processes after 12 h.Seed and river sand mix ratio are 1:3.
Advantage of the present invention is: 1, take the mode of artificial after-ripening to process seed, to reach the target of embryo morphogenesis and physiology after-ripening, and physiology after-ripening only the cryopreservation of the seed appropriate time of embryo complete form can sow germination.Through this method, process, adonis amurensis seed can develop into heart-shape embryo by globular embryo, is finally developed to ripe cotyledonary embryos seed.Then by low temperature treatment, break cotyledonary embryos dormancy, realize and sprouting.2, result of the present invention provides high-quality adonis amurensis seedling significant for further artificial large-scale planting.
Below in conjunction with embodiment, embodiments of the present invention are described in further detail.
Embodiment
The quick after-ripening method of adonis amurensis seed, its step is as follows:
1 materials and methods
1.1 experiment materials and processing
Spring (the first tenday period of a month in May), gather adonis amurensis aggregate fruit ball, after being dried, sloughs after kind of skin by aggregate fruit ball, by 2 of different quality concentration, 4-dichlorphenoxyacetic acid (2,4-D) and in 6-benzyl aminopurine (6-BA) mixed solution soak after 48 h, seed and river sand (1:2) are mixed in the trogue that all packs afterwards good permeability into, covered with plastic film is placed in the indoor alternating temperature processing of carrying out.
The morphogenesis of 1.2 adonis amurensis embryos, physiology after-ripening and sowing
By the seed of processing in 1.1, wherein, the mass concentration of 2,4-D solution is controlled at 0.50～2.50 mgL
-1, the mass concentration of 6-BA solution is controlled at 0.20～0.40 mgL
-1, alternating temperature amplitude and time: day temperature is 20 ± 2 ℃, process 12 h, nocturnal temperature is 10 ± 2 ℃, processes 12 h.Processing the time limit is 25～81 d, every 7 d get seed and observe embryo form during this time, by every 50 seed morphologies, growing to next stage embryo's percentage is investigation object, it is heart-shape embryo incidence=heart-shape embryo number/50 * 100%, cotyledonary embryos incidence=cotyledonary embryos number/50 * 100%, for improving speed and the incidence of adonis amurensis embryo morphogenesis, by Uniform Design method, test, select U
9(9
3) evenly show, each processes 50 seeds, repeats 3 times and averages, and determines in adonis amurensis embryo morphogenesis process mass concentration and the processing time of 2,4-D, 6-BA solution.
2 results and analysis
The impact that 2.1 2,4-D, 6-BA and alternating temperature processing time occur adonis amurensis seed heart-shape embryo
Experiment the data obtained (table 1) can obtain regression equation after uniform Design software analysis is processed
y=56.6+5.83
x 1-+35.4
x 2+ 0.148
x 3, significance
α=0.05, multiple correlation coefficient
r=0.9736, residual standard deviation
s=1.4900, test value
f t =30.29 ﹥ critical values
f (0.05,3,5)=5.409, regression equation has significance, and the impact that 2,4-D, 6-BA and alternating temperature processing time occur adonis amurensis embryo heart-shape embryo all has significance.It is known to contribution margin and the contribution rate of the generation of adonis amurensis embryo heart-shape embryo by calculating 2,4-D, 6-BA and alternating temperature processing time,
u 1=126,
u 1/
u=62.2%;
u 2=66.7,
u 2/
u=33.0%;
u 3=57.8,
u 3/
u=28.6%, illustrate that 2,4-D is greater than 6-BA and the contribution of alternating temperature processing time to the generation of adonis amurensis embryo heart-shape embryo, 2,4-D plays a leading role, and 6-BA and alternating temperature are processed the indispensable effect of playing.Because of 2,4-D, 6-BA and alternating temperature processing time and heart-shape embryo incidence are all proportionate, and infer that 2,4-D and 6-BA mass concentration are respectively higher than 2.50 mgL
-1with 0.40 mgL
-1, the alternating temperature processing time, while surpassing 81 d, adonis amurensis seed heart-shape embryo incidence may be higher.For verifying this possibility, therefore, with 2,4-D mass concentration, be 2.50,2.75,3.00,3.25 and 3.50 mgL again
-1, 6-BA mass concentration is 0.40,0.50,0.60,0.70,0.80,0.90 and 1.00 mgL
-1, the alternating temperature processing time is the complementary testing that 81,88,95,102,109,116 and 123 d carry out 7 processing, repeats 3 times.Operating process, with 1.1 and 1.2 methods, found that 2,4-D mass concentration is (2.50～3.00 mgL
-1), 6-BA mass concentration is (0.50～0.70 mgL
-1) and the alternating temperature processing time while being (81～102 d) heart-shape embryo incidence the highest, average originating rate reaches more than 99.3%.All high than the incidence of listed 9 processing of table 1.
Table 1
the U of adonis amurensis embryo morphogenesis stage principal element screening
9(9
3) experimental scheme and result
Table1 U
9(9
3)test design and result of factors for embryo morphogenesis of seeds of
Adonis amurensis
The impact that 2.2 2,4-D, 6-BA and alternating temperature processing time occur adonis amurensis seed cotyledonary embryos
Data (table 1) can obtain regression equation after processing by analysis
y=31.3+92.3
x 2-+0.419
x 3, significance
α=0.05, multiple correlation coefficient
r=0.8923, residual standard deviation
s=5.4700, test value
f t =11.72 ﹥ critical values
f (0.05,2,6)=5.143, regression equation has significance, and the impact that 6-BA and alternating temperature processing time occur adonis amurensis embryo cotyledonary embryos has significance, and the impact that 2,4-D occurs adonis amurensis embryo cotyledonary embryos is not remarkable.In like manner, contribution margin and contribution rate that calculating 6-BA and alternating temperature processing time occur adonis amurensis embryo cotyledonary embryos are known,
u 2=460,
u 2/
u=65.5%;
u 3=464,
u 3/
u=66.1%, although the alternating temperature processing time is greater than the contribution that 6-BA occurs adonis amurensis embryo cotyledonary embryos, but from contribution rate numerical value, 6-BA and alternating temperature processing time gap are little, illustrate that 6-BA and alternating temperature processing time are all the factors playing an important role to the contribution of adonis amurensis embryo cotyledonary embryos generation.Because 6-BA and alternating temperature processing time and cotyledonary embryos incidence are all proportionate, to infer thus, 6-BA mass concentration is higher than 0.40 mgL
-1process and may have higher cotyledonary embryos incidence by adonis amurensis with the alternating temperature that surpasses 81 d.For verifying this supposition, take again 6-BA mass concentration as 0.40,0.50,0.60,0.70,0.80,0.90 and 1.00 mgL
-1, the alternating temperature processing time is the complementary testing that 81,88,95,102,109,116 and 123 d carry out 7 processing, repeats 3 times.Operating process, with 1.1 and 1.2 methods, found that 6-BA mass concentration is for (0.60～0.90 mgL
-1) and the alternating temperature processing time while being (88～109 d) heart-shape embryo incidence the highest, average originating rate reaches more than 99.0%, all higher than the cotyledonary embryos incidence of listed 10 processing of table 1.
3 conclusions
Known by above Data Processing in Experiment and analysis, owing to affecting factor and the scope of heart-shape embryo, be that 2,4-D mass concentration is (2.50～3.00 mgL
-1), 6-BA mass concentration is (0.50～0.70 mgL
-1) and the alternating temperature processing time be (81～102 d), and affect the factor of cotyledonary embryos and scope is that 6-BA mass concentration is (0.60～0.90 mgL
-1) and the alternating temperature processing time be (88～109 d).
Therefore the condition that, can determine adonis amurensis embryo morphogenesis is: 2.50～3.00 mgL for seed
-12,4-D and 0.60～0.70 mgL
-16-BA mixed solution pull out after soaking 48 h, by seed and river sand
(1:3)mix in the trogue that all packs afterwards good permeability into, covered with plastic film is placed in the indoor alternating temperature that carries out 88～109 d and processes.Alternating temperature amplitude and time: day temperature is 20 ± 2 ℃, process 12 h, nocturnal temperature is 10 ± 2 ℃, processes after 12 h, adonis amurensis seed can develop into heart-shape embryo by globular embryo, is finally developed to ripe cotyledonary embryos seed.
Claims (2)
1. the quick after-ripening method of adonis amurensis seed, is characterized in that step is as follows: after adonis amurensis aggregate fruit ball is dried, slough after kind of skin, through 2,4-dichlorphenoxyacetic acid, 2.50～3.00 mgL
-1with 6-benzyl aminopurine 0.60～0.70 mgL
-1in mixed solution, soak after 48 h, seed and river sand are mixed in the trogue that all packs afterwards good permeability into, covered with plastic film is placed in the indoor alternating temperature carrying out 88～109 d days and processes, and alternating temperature amplitude and time are: day temperature is 20 ± 2 ℃, processing times 12 h; Nocturnal temperature is 10 ± 2 ℃, processing times 12 h; Finally be developed to ripe cotyledonary embryos seed, realize and sprouting.
2. according to the quick after-ripening method of adonis amurensis seed claimed in claim 1, it is characterized in that seed and river sand mix ratio are 1:3.
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| WO2011145112A1 (en) * | 2010-05-17 | 2011-11-24 | Dynadis Biotehc (India) Private Limited | Process for isolation and purification of carotenoids |
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| WO2011145112A1 (en) * | 2010-05-17 | 2011-11-24 | Dynadis Biotehc (India) Private Limited | Process for isolation and purification of carotenoids |
Non-Patent Citations (3)
| Title |
|---|
| 段金玉 等.在无菌条件下，激素和种子处理对兰属十种植物种子萌发的影响.《云南植物研究》.1982,第4卷(第2期), * |
| 潘春柳 等.禺毛茛种子贮藏与萌发特性的研究.《种子》.2011,第30卷(第11期), |
| 禺毛茛种子贮藏与萌发特性的研究;潘春柳 等;《种子》;20111130;第30卷(第11期);第101-103页 * |
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