CN102965030A - Preparation method of high-gel-strength fish scale gelatin - Google Patents
Preparation method of high-gel-strength fish scale gelatin Download PDFInfo
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- CN102965030A CN102965030A CN2012104352301A CN201210435230A CN102965030A CN 102965030 A CN102965030 A CN 102965030A CN 2012104352301 A CN2012104352301 A CN 2012104352301A CN 201210435230 A CN201210435230 A CN 201210435230A CN 102965030 A CN102965030 A CN 102965030A
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Abstract
The invention relates to a preparation method of a high-gel-strength fish scale gelatin, which comprises the following step: performing ammonium sulfate salting-out on a fish scale gelatin, which is prepared from fish scale waste and used as an object, to prepare a high-gel-strength fish scale gelatin product. The preparation method provided by the invention has the following positive effects: 1, the fish scale waste is used as an initial raw material for gelatin preparation, thus realizing the high-value utilization of the resource, protecting the environment, realizing the sustainable green development, effectively reducing the gelatin production cost and reducing the gelatin import volume of China; 2, the fish scale gelatin is subjected to ammonium sulfate salting-out for the first time, thus preparing the high-gel-strength fish scale gelatin, reducing the consumption in the application process and further reducing the gelatin application cost; and 3, the method for preparing a high-gel-strength fish scale gelatin through ammonium sulfate salting-out can provide a practical and feasible basis for the preparation of a high-quality gelatin, thereby laying a foundation for the further study on the gelatin and making a little contribution for the quick development of the gelatin industry in China.
Description
Technical field
The present invention relates to a kind of preparation method of fish scale gelatin, relate in particular to a kind of preparation method of high-gel strength fish scale gelatin.
Background technology
Gelatin is a kind of multi-functional water-soluble colloid, can be widely used in the fields such as food, medicine, makeup, photograph.In recent years, fast development along with China's industry, the gelatin demand sharply increases, because of the problem such as food gelatin insufficient raw material and production cost be higher, many gelatin enterprises of China are sold to radiopharmacy to produce capsule for medicine with technical gelatine, caused " toxic capsule " event that everybody knows, serious harm people healthy.
Based on above-mentioned present situation, the present invention is take fish scale gelatin as object, adopt ammonium sulfate salting-out process, preparation high-gel strength fish scale gelatin, not only expanded edible Gelatinum oxhide the source, remedied the problem of insufficient raw material, and usage quantity procurement price few, the initial feed fish scale is low when using because of it, effectively reduces production and the application cost of fish scale gelatin end product.
The method for preparing the high-gel strength fish scale gelatin by ammonium sulfate among the present invention there is not yet the pertinent literature report at home and abroad.
Summary of the invention
The present invention aims to provide a kind of preparation method of high-gel strength fish scale gelatin, take fish scale gelatin as object, by the effective control to the ammonium sulfate saturation ratio, prepares the fish scale gelatin of high-gel strength, to reach the purpose that improves the end product quality.
The present invention is achieved in that its concrete technology scheme is as follows:
1, the preparation of initial fish scale gelatin: (1) tissue mashing: after removing non-fish scale material, processed fish scale 3-6 minute with tissue mashing machine, clean again to clarification of water and be advisable; (2) fish scale decalcification: adopt hydrochloric acid that fish scale is carried out decalcification, technique is 0.3mol/L-0.8mol/L concentration of hydrochloric acid, solid-liquid ratio 1:10-1:30, decalcification time 0.5h-2h, after decalcification finishes fish scale is cleaned to approaching neutral; (3) fish scale gelatin preparation: adopt water law to prepare fish scale gelatin, technique is solid-liquid ratio 1:5-1:20,70 ℃-90 ℃ of bath temperatures, time 1h-3h; (4) spraying drying: 180 ℃-200 ℃ of inlet temperature, 80 ℃-90 ℃ of air outlet temperatures obtain the fish scale gelatin primary products;
2, the preparation of high-gel strength fish scale gelatin product: the solution preparation of (1) glue: fish scale gelatin is mixed with 2%-10%(w/v), after being lower than under 60 ℃ the temperature fully dissolving, with clarifixator homogeneous under 20-60Mpa, obtain the fish scale gelatin solution of abundant mixing; (2) pre-treatment of ammonium sulfate: according to saturation ratio 15%-35%, calculate the ammonium sulfate solids amount of good required adding, place 80-100 ℃ baking oven heating 2h to remove moisture in the ammonium sulfate that weighs up, promote the dissolving of ammonium sulfate in fish scale gelatin solution; (3) ammonium sulfate precipitation: pretreated ammonium sulfate solids is added in the fish scale gelatin solution, and the limit edged stirs, and makes that ammonium sulfate reaches required saturation ratio in the solution; (4) leave standstill: will be added with the fish scale gelatin solution left standstill 4-12h of ammonium sulfate, the fish scale gelatin of high-gel strength is separated out as far as possible; (5) centrifugal: the fish scale gelatin solution with whizzer layering is centrifugal under 3000-4000r/min, and centrifugal rear abandoning supernatant gets the fish scale gelatin precipitation; (6) dialysis: fish scale gelatin precipitation is redissolved, is contained in the dialysis tubing, in 25 ℃ flowing water, place 20-30h, can not make barium chloride solution become muddiness to dialyzate till; (7) spraying drying: 180 ℃-200 ℃ of inlet temperature, 80 ℃-90 ℃ of air outlet temperatures obtain high-gel strength fish scale gelatin product.
3, detect: the index of detection comprises gel-strength, physical and chemical index and microbiological indicator.
The feature of this product: the gelatin for preparing in the waste fish scale is as raw material, according to ammonium sulfate precipitation protein principle, ammonium sulfate precipitation is used for the preparation of high-gel strength fish scale gelatin, energy Effective Raise product quality, reduce its usage quantity in application process, reduce production and the application cost of gelatin.
Positively effect of the present invention: 1, preparing gelatin take the fish scale waste as initial feed is higher value application to resource, can not only protection of the environment, realize sustainable Green Development, and can effectively reduce the production cost of gelatin, reduce the import volume of China's gelatin; 2, use first ammonium sulfate salting-out process to the fish scale gelatin effect, prepare the fish scale gelatin of high-gel strength, reduced its usage quantity in application process, further reduced the application cost of gelatin; 3, the preparation that the method that adopts ammonium sulfate precipitation to prepare the high-gel strength fish scale gelatin can be high-quality gelatin provides truly feasible foundation, for the further further investigation of gelatin lays the foundation, for the fast development of China's gelatin industry is done one's bit.
Description of drawings
Fig. 1 is process flow sheet of the present invention.
Embodiment
Embodiment one
1, the preparation of initial fish scale gelatin: (1) tissue mashing: after removing non-fish scale material, processed fish scale 6 minutes with tissue mashing machine, clean again to clarification of water and be advisable; (2) fish scale decalcification: adopt hydrochloric acid that fish scale is carried out decalcification, technique is 0.5mol/L concentration of hydrochloric acid, solid-liquid ratio 1:20, decalcification time 1h, after decalcification finishes fish scale is cleaned to approaching neutral; (3) fish scale gelatin preparation: adopt the hot-water process to prepare fish scale gelatin, technique is solid-liquid ratio 1:20,80 ℃ of bath temperatures, time 3h; (4) spraying drying: 180 ℃ of inlet temperature, 90 ℃ of air outlet temperatures obtain the fish scale gelatin primary products.
2, the preparation of high-gel strength fish scale gelatin product: the preparation of (1) glue: fish scale gelatin is mixed with 4% solution, after being lower than under 60 ℃ the temperature fully dissolving, with clarifixator homogeneous under 30Mpa, obtains the fish scale gelatin solution of abundant mixing; (2) pre-treatment of ammonium sulfate: according to saturation ratio 20%, calculate the ammonium sulfate solids amount of good required adding, place 100 ℃ baking oven heating 2h to remove moisture in the ammonium sulfate that weighs up, promote the dissolving of ammonium sulfate in fish scale gelatin solution; (3) ammonium sulfate precipitation: pretreated ammonium sulfate solids is added in the fish scale gelatin solution, and the limit edged stirs, and makes that ammonium sulfate reaches required saturation ratio in the solution; (4) leave standstill: will be added with the fish scale gelatin solution left standstill 6h of a certain amount of ammonium sulfate, the fish scale gelatin of high-gel strength is separated out as far as possible; (5) centrifugal: the fish scale gelatin solution with whizzer layering is centrifugal under 4000r/min, and centrifugal rear abandoning supernatant gets the fish scale gelatin precipitation; (6) dialysis: fish scale gelatin precipitation is redissolved, is contained in the dialysis tubing, place in the flowing water about 25 ℃ about 24h, can not make barium chloride solution become muddiness to dialyzate till; (7) the spraying inlet temperature is 180 ℃, and 90 ℃ of air outlet temperatures obtain high-gel strength fish scale gelatin product
3, detect: the physical and chemical index and the microbiological indicator that detect the fish scale gelatin end product.
4, gel-strength: the gel-strength of fish scale gelatin end product exceeds 10%-20% than initial fish scale gelatin.
Embodiment two
1, the preparation of initial fish scale gelatin: (1) tissue mashing: after removing non-fish scale material, processed fish scale 6 minutes with tissue mashing machine, clean again to clarification of water and be advisable; (2) fish scale decalcification: adopt hydrochloric acid that fish scale is carried out decalcification, technique is 0.5mol/L concentration of hydrochloric acid, solid-liquid ratio 1:20, decalcification time 1h, after decalcification finishes fish scale is cleaned to approaching neutral; (3) fish scale gelatin preparation: adopt the hot-water process to prepare fish scale gelatin, technique is solid-liquid ratio 1:20,80 ℃ of bath temperatures, time 3h; (4) spraying drying: 180 ℃ of inlet temperature, 90 ℃ of air outlet temperatures obtain the fish scale gelatin primary products.
2, the preparation of high-gel strength fish scale gelatin product: the preparation of (1) glue: fish scale gelatin is mixed with 4% solution, after being lower than under 60 ℃ the temperature fully dissolving, with clarifixator homogeneous under 30Mpa, obtains the fish scale gelatin solution of abundant mixing; (2) pre-treatment of ammonium sulfate: according to saturation ratio 25%, calculate the ammonium sulfate solids amount of good required adding, place 100 ℃ baking oven heating 2h to remove moisture in the ammonium sulfate that weighs up, promote the dissolving of ammonium sulfate in fish scale gelatin solution; (3) ammonium sulfate precipitation: pretreated ammonium sulfate solids is added in the fish scale gelatin solution, and the limit edged stirs, and makes that ammonium sulfate reaches required saturation ratio in the solution; (4) leave standstill: will be added with the fish scale gelatin solution left standstill 6h of a certain amount of ammonium sulfate, the fish scale gelatin of high-gel strength is separated out as far as possible; (5) centrifugal: the fish scale gelatin solution with whizzer layering is centrifugal under 4000r/min, and centrifugal rear abandoning supernatant gets the fish scale gelatin precipitation; (6) dialysis: fish scale gelatin precipitation is redissolved, is contained in the dialysis tubing, place in the flowing water about 25 ℃ about 24h, can not make barium chloride solution become muddiness to dialyzate till; (7) spraying drying: 180 ℃ of inlet temperature, 90 ℃ of air outlet temperatures obtain high-gel strength fish scale gelatin product
3, detect: the physical and chemical index and the microbiological indicator that detect the fish scale gelatin end product.
4, gel-strength: the gel-strength of fish scale gelatin end product exceeds 5%-10% than initial fish scale gelatin.
Claims (1)
1. the fish scale gelatin preparation method of a high-gel strength is characterized in that method is:
1) preparation of initial fish scale gelatin: (1) tissue mashing: after removing non-fish scale material, processed fish scale 3-6 minute with tissue mashing machine, clean again to clarification of water and be advisable; (2) fish scale decalcification: adopt hydrochloric acid that fish scale is carried out decalcification, technique is 0.3mol/L-0.8mol/L concentration of hydrochloric acid, solid-liquid ratio 1:10-1:30, decalcification time 0.5h-2h, after decalcification finishes fish scale is cleaned to approaching neutral; (3) fish scale gelatin preparation: adopt the hot-water process to prepare fish scale gelatin, technique is solid-liquid ratio 1:5-1:20,70 ℃-90 ℃ of bath temperatures, time 1h-3h; (4) spraying drying: 180 ℃-200 ℃ of inlet temperature, 80 ℃-90 ℃ of air outlet temperatures obtain the fish scale gelatin primary products;
2) preparation of high-gel strength fish scale gelatin product: the solution preparation of (1) glue: fish scale gelatin is mixed with 2%-10%(w/v), after being lower than under 60 ℃ the temperature fully dissolving, with clarifixator homogeneous under 20-60Mpa, obtain the fish scale gelatin solution of abundant mixing; (2) pre-treatment of ammonium sulfate: according to saturation ratio 15%-35%, calculate the ammonium sulfate solids amount of good required adding, place 80-100 ℃ baking oven heating 2h to remove moisture in the ammonium sulfate that weighs up, promote the dissolving of ammonium sulfate in fish scale gelatin solution; (3) ammonium sulfate precipitation: pretreated ammonium sulfate solids is added in the fish scale gelatin solution, and the limit edged stirs, and makes that ammonium sulfate reaches required saturation ratio in the solution; (4) leave standstill: will be added with the fish scale gelatin solution left standstill 4-12h of ammonium sulfate, the fish scale gelatin of high-gel strength is separated out as far as possible; (5) centrifugal: the fish scale gelatin solution with whizzer layering is centrifugal under 3000-4000r/min, and centrifugal rear abandoning supernatant gets the fish scale gelatin precipitation; (6) dialysis: fish scale gelatin precipitation is redissolved, is contained in the dialysis tubing, in 25 ℃ flowing water, place 20-30h, can not make barium chloride solution become muddiness to dialyzate till; (7) spraying drying: 180 ℃-200 ℃ of inlet temperature, 80 ℃-90 ℃ of air outlet temperatures obtain high-gel strength fish scale gelatin product.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103289575A (en) * | 2013-06-25 | 2013-09-11 | 南昌大学 | Preparation method of fish scale gelatin of bighead carp |
| CN104342040A (en) * | 2014-10-09 | 2015-02-11 | 江西师范大学 | Preparation method of high-melt-temperature fish scale gelatin |
| CN108485534A (en) * | 2018-04-12 | 2018-09-04 | 浙江海洋大学 | A kind of preparation method of Japanese croaker fish scale gelatin |
| CN109221877A (en) * | 2018-08-31 | 2019-01-18 | 苏炳京 | Health-care steeping pot-stewed chicken pawl and its processing method |
Citations (4)
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| GB625873A (en) * | 1946-02-07 | 1949-07-05 | Kodak Ltd | Method of preparing gelatine |
| GB845274A (en) * | 1955-10-06 | 1960-08-17 | Gen Foods Corp | Improved gelatin composition |
| SU979475A1 (en) * | 1981-06-19 | 1982-12-07 | Казанский Фотожелатиновый Завод | Process for producing gelatin |
| CN1162971A (en) * | 1994-11-07 | 1997-10-22 | 沃尼尔·朗伯公司 | Process for stabilizng gelatin products |
-
2012
- 2012-11-05 CN CN201210435230.1A patent/CN102965030B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB625873A (en) * | 1946-02-07 | 1949-07-05 | Kodak Ltd | Method of preparing gelatine |
| GB845274A (en) * | 1955-10-06 | 1960-08-17 | Gen Foods Corp | Improved gelatin composition |
| SU979475A1 (en) * | 1981-06-19 | 1982-12-07 | Казанский Фотожелатиновый Завод | Process for producing gelatin |
| CN1162971A (en) * | 1994-11-07 | 1997-10-22 | 沃尼尔·朗伯公司 | Process for stabilizng gelatin products |
Non-Patent Citations (1)
| Title |
|---|
| 位绍红: "罗非鱼鱼皮、鱼鳞提取明胶的工艺研究", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103289575A (en) * | 2013-06-25 | 2013-09-11 | 南昌大学 | Preparation method of fish scale gelatin of bighead carp |
| CN104342040A (en) * | 2014-10-09 | 2015-02-11 | 江西师范大学 | Preparation method of high-melt-temperature fish scale gelatin |
| CN104342040B (en) * | 2014-10-09 | 2016-03-02 | 江西师范大学 | A kind of high glue melts the preparation method of temperature fish scale gelatin |
| CN108485534A (en) * | 2018-04-12 | 2018-09-04 | 浙江海洋大学 | A kind of preparation method of Japanese croaker fish scale gelatin |
| CN109221877A (en) * | 2018-08-31 | 2019-01-18 | 苏炳京 | Health-care steeping pot-stewed chicken pawl and its processing method |
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| CN102965030B (en) | 2014-05-14 |
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