CN106800584B - The method of protein is recycled from Dai Yu Yu Porridge rinsing liquid - Google Patents
The method of protein is recycled from Dai Yu Yu Porridge rinsing liquid Download PDFInfo
- Publication number
- CN106800584B CN106800584B CN201611167844.0A CN201611167844A CN106800584B CN 106800584 B CN106800584 B CN 106800584B CN 201611167844 A CN201611167844 A CN 201611167844A CN 106800584 B CN106800584 B CN 106800584B
- Authority
- CN
- China
- Prior art keywords
- porridge
- rinsing liquid
- protein
- dai
- fish
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
The present invention discloses a kind of method that protein is recycled from Dai Yu Yu Porridge rinsing liquid, it is characterised in that: the composite flocculation agent that chitosan and cationic polyacrylamide composition are added into Dai Yu Yu Porridge rinsing liquid is uniformly mixed, centrifuging and taking supernatant simultaneously collects precipitating；The anionic polyacrylamide as flocculant is added in clear liquid then up, uniformly mixed, centrifuging and taking supernatant simultaneously collects precipitating；Precipitating twice merges, is dried to obtain protein.The present invention has many advantages, such as that simple process, processing cost are low, the period is short, and for reducing the COD (COD) with fish fish Porridge rinsing liquid, protection environment is of great significance.
Description
Technical field
The present invention relates to field of biotechnology, in particular to a kind of utilization chitosan-cationic polyacrylamide, anion
The method that the flocculants such as polyacrylamide recycle protein from Dai Yu Yu Porridge rinsing liquid.
Technical background
Hairtail also known as hairtail, dental strip fish are net-rope Perciformes Trichiuridae animals, are a kind of higher warps of China coast yield
Ji fish.Band fish protein is a kind of complete protein, full of nutrition containing eight kinds of amino acid needed by human.The hairtail flesh of fish
It is white, it is the very good material that processing prepares Yu Porridge.Minced fillet is by fish by adopting meat, rinsing, refined filtration, dehydration stirring and freezing to add
The product that work obtains is to prepare Yu Porridge product such as: fish ball, fish sausage, paupiette raw material.Surimi product does not have fishy smell, rich bullet
Property, it is unique flavor, full of nutrition and cheap, it is deep to be liked by masses.In Yu Porridge process, rinsing is one of key
Process is directly related to the gel strength and whiteness of Yu Porridge, plays an important role to raising surimi product quality and keeping property.
Rinsing is usually the water that 3~5 times or so flesh of fish weight is added, and mixes slowly, stands, make after being fully dissolved out water-solubility protein etc.
The flesh of fish sufficiently sinks, and recycles upper layer fish oil, and incline rinsing liquid, then adds water according to the above ratio, repeats rinsing 1~3 time；Thus may be used
See, rinsing process water consumption is very big, and the water soluble protein for accounting for fish protein 30%~40% is caused to enter rinsing liquid, is formed big
Eutrophy processing waste water is measured, some researches show that the CODs (COD) of , Yu Porridge rinsing liquid to be up to 8000-10000mg/L, such as
Fruit is untreated will to generate serious pollution with regard to discharge to environment.Therefore, protein is recycled in Cong Yu Porridge rinsing liquid, for reducing
COD, protection environment are of great significance.
The common methods for recycling protein from surimi washings at present mainly have: heating, isoelectric point precipitation, flocculation
Method etc..Heating energy consumption is high, does not meet the requirement of energy-saving and emission-reduction, and increase the cost of enterprise.Isoelectric point precipitation is to pass through
It reconciles and is allowed to precipitate near the pH to the isoelectric point of water soluble protein of rinsing liquid.Due to the equal electricity of protein in Yu Porridge rinsing liquid
Rinsing liquid of the point generally behind pH4.2~5.5, isoelectric point method protein precipitation is in highly acid, if direct emission will cause water body
Acidification pollution environment.Flocculence is that a certain amount of flocculant is added into surimi washings, so that protein is occurred de- steady and agglomerates
It settles down at bulky grain, achievees the purpose that recycle protein；Flocculence has many advantages, such as that operating process is simple, low in cost,
It is a kind of excellent Protein Recovery method.
Due to containing multiple proteins in surimi washings, and traditional flocculence often only collects single isoelectric point
Protein causes wherein also containing the protein not being recovered largely, this part surimi washings is discharged into environment, can make
It is high at COD, serious to environment generation to pollute.But still also non-someone is for different in Dai Yu Yu Porridge rinsing liquid at present
The protein of isoelectric point carries out the report of flocculence recycling.
Summary of the invention
The present invention is directed to the above-mentioned deficiency of the prior art, provide it is a kind of using different flocculants, for different isoelectric points
Protein recycled, to recycle protein to greatest extent, reduce the COD of fish Porridge rinsing liquid, protect environment from hairtail fish
The method of protein is recycled in Porridge rinsing liquid.
In order to solve the above-mentioned technical problem, the technical solution adopted by the present invention are as follows: one kind is returned from Dai Yu Yu Porridge rinsing liquid
The method for receiving protein, wherein adding the compound wadding of chitosan and cationic polyacrylamide composition into Dai Yu Yu Porridge rinsing liquid
Solidifying agent, uniformly mixed, centrifuging and taking supernatant simultaneously collect precipitating；It is added in clear liquid as the anion of flocculant poly- third then up
Acrylamide, uniformly mixed, centrifuging and taking supernatant simultaneously collect precipitating；Precipitating twice merges, is dried to obtain protein.
The above-mentioned Dai Yu Yu Porridge rinsing liquid of the present invention, it is specific the preparation method comprises the following steps: by the hairtail flesh of fish (decaptitating, tail and interior
It is dirty) it rubs, the flesh of fish is then added to 4 DEG C as rinsing liquid, in 0.3% (mass percent concentration) NaCl solution, it is first slow
Speed stirring 5~15 minutes, then filtered after standing 10~30 minutes with multilayer gauze (multilayer gauze can be 3-6 layers), obtain fish
Porridge rinsing liquid, above-mentioned rinse cycle repeat 1~3 time, and the filtrate for merging repeatedly rinsing filtering obtains fish Porridge rinsing liquid.
Concentration of the above-mentioned chitosan and cationic polyacrylamide of the present invention in Dai Yu Yu Porridge rinsing liquid is respectively
1.0g/L~3.0g/L, 0.02g/L~0.08g/L.
Above-mentioned chitosan is that the form for the chitosan solution for being first 1% at mass concentration with peracetic acid formulation is added to hairtail
In Yu Porridge rinsing liquid.
Above-mentioned cationic polyacrylamide is first to be configured to the cation polypropylene acyl that mass concentration is 0.025% with water
The form of amine aqueous solution is added in Dai Yu Yu Porridge rinsing liquid.
Concentration of the above-mentioned anionic polyacrylamide for being added to supernatant of the present invention in supernatant be 0.025g/L~
0.075g/L。
The above-mentioned anionic polyacrylamide of the present invention be first with water be configured to yin that concentration of polymer solution is 0.025% from
The form of sub- polyacrylamide solution is added in supernatant.
The above-mentioned centrifugation of the present invention, specifically are as follows: be centrifuged 10~15 minutes at 3500~4000rpm, 1~5 DEG C, then
It takes supernatant and collects precipitating.
The above-mentioned drying of the present invention, specifically by precipitating be placed in 50~60 DEG C, dry 12 under the conditions of 0.08~0.1MPa~
It 24 hours, is then crushed up to protein after the recovery.
The above-mentioned method that protein is recycled from Dai Yu Yu Porridge rinsing liquid of the present invention, specific steps include:
(1) the hairtail flesh of fish is rubbed into (decaptitating, tail and internal organ, adopt meat by hand) and weighs weight；With 4 DEG C, 0.3%NaCl solution
As rinsing liquid, the weight ratio of the control flesh of fish and NaCl solution is 1:3~5 when rinsing, and it is molten that the flesh of fish is added to above-mentioned NaCl
It in liquid, first mixes slowly 5~15 minutes, then with multilayer gauze (multilayer gauze can be 3-6 layers) mistake after standing 10~30 minutes
Filter repeats rinsing 1~3 time, obtains Yu Porridge rinsing liquid；
(2) prepared to step (1) be added in Yu Porridge rinsing liquid 1.0g/L~3.0g/L chitosan and 0.02g/L~
0.08g/L cationic polyacrylamide, stirs evenly, and is then allowed to stand 30~120 minutes and obtains mixture；
(3) step (2) resulting mixture is centrifuged, 3500~4000rpm, 1~5 DEG C, 10~15 minutes take
Clear liquid simultaneously collects precipitating；
(4) 0.025g/L~0.075g/L anionic polyacrylamide, stirring are added into step (3) resulting supernatant
Uniformly, it is then allowed to stand 30~120 minutes and obtains mixture；
(5) step (4) resulting mixture is centrifuged, 3500~4000rpm, 1~5 DEG C, 10~15 minutes take
Clear liquid simultaneously collects precipitating；
(6) precipitating that collection step (3) and step (5) obtain, 50~60 DEG C, 0.08~0.1MPa, dry 12~24 is small
Shi Hou is crushed up to protein after the recovery.
The present invention detects the protein concentration of Yu Porridge rinsing liquid and supernatant using conventional biuret method, and according to following
Formula calculates protein recovery.Protein recovery of the invention is 85~95%.
R (%): protein recovery；
C0: surimi washings protein concentration；V0: surimi washings volume
C: finally obtained supernatant protein concentration；V: finally obtained supernatant volume
The present invention have following remarkable advantage and the utility model has the advantages that
(1) present invention carries out flocculence recycling, applicant for the protein of isoelectric points different in Dai Yu Yu Porridge rinsing liquid
Early-stage study discovery, the isoelectric point of the protein in Dai Yu Yu Porridge rinsing liquid are pH4~6 and pH9~10, compound using first adding
Flocculant (chitosan-cationic polyacrylamide) recycles isoelectric point and is the protein of pH4~6, then adds anion pp
Amide recycles the protein that isoelectric point is pH9~10；With only for isoelectric points of proteins acid pH or alkaline pH absorption method
It compares, the present invention realizes the maximum recycling of Dai Yu Yu Porridge rinsing liquid protein.
(2) present invention first uses composite flocculation agent (chitosan-cationic polyacrylamide) to isoelectric point in pH4~6
Protein carries out coagulation and recovery, compared with a kind of flocculant (chitosan or cationic polyacrylamide) is used only, improves egg
The rate of recovery of white matter.
(3) present invention utilizes addition chitosan-cationic polyacrylamide, anion pp acyl into Yu Porridge rinsing liquid
The method of the flocculants such as amine recycles protein, has many advantages, such as that simple process, processing cost are low, the period is short, for reducing hairtail
The COD (COD) of fish Porridge rinsing liquid, protection environment are of great significance.
Specific embodiment
4 preferred embodiments are given below, but the present invention is not only limited to following embodiment.
Embodiment 1:
By hairtail decaptitating, tail and internal organ, meat is adopted by hand, is rubbed, is weighed flesh of fish 1kg, with 4 DEG C, 0.3%NaCl solution conduct
Rinsing liquid, the ratio of the control flesh of fish and rinsing liquid is 1:3 when rinsing, i.e., the 1kg flesh of fish is added in 3kg rinsing liquid, first at a slow speed
Stirring 7 minutes, then repeated to rinse 1 time, obtained Yu Porridge rinsing liquid with four layers of filtered through gauze after standing 12 minutes；It is rinsed Xiang Yu Porridge
1.0g/L chitosan (with peracetic acid formulation at solution, concentration 1%) and 0.02g/L cationic polyacrylamide (use are added in liquid
Water is configured to solution, concentration 0.025%), it stirs evenly, is centrifuged after standing 45 minutes: 3800rpm, 3 DEG C, 10 minutes,
It takes supernatant and collects precipitating；0.025g/L anionic polyacrylamide is added into supernatant and (is configured to solution, concentration with water
0.025%), to stir evenly, be centrifuged after standing 30 minutes: 3800,3 DEG C, 10 minutes take supernatant and collect precipitating；
Merge 2 times and be centrifuged obtained precipitating, 50 DEG C, 0.095MPa, after 20 hours dry, is crushed up to protein after the recovery.
Using the protein concentration of biuret method detection Yu Porridge rinsing liquid and supernatant, it is computed and show that protein recovery is
86.2%.
Embodiment 2:
By hairtail decaptitating, tail and internal organ, meat is adopted by hand, is rubbed, is weighed flesh of fish 2kg, with 4 DEG C, 0.3%NaCl solution conduct
Rinsing liquid, the ratio of the control flesh of fish and rinsing liquid is 1:4 when rinsing, i.e., the 2kg flesh of fish is added in 8kg rinsing liquid, first at a slow speed
Stirring 8 minutes, then repeated to rinse 2 times, obtained Yu Porridge rinsing liquid with four layers of filtered through gauze after standing 15 minutes；It is rinsed Xiang Yu Porridge
2.0g/L chitosan (with peracetic acid formulation at solution, concentration 1%) and 0.06g/L cationic polyacrylamide (use are added in liquid
Water is configured to solution, concentration 0.025%), it stirs evenly, standing is centrifuged after sixty minutes: 4000rpm, 4 DEG C, 15 minutes,
It takes supernatant and collects precipitating；0.05g/L anionic polyacrylamide is added into supernatant and (is configured to solution, concentration with water
0.025%), to stir evenly, be centrifuged after standing 90 minutes: 4000,3 DEG C, 12 minutes take supernatant and collect precipitating；
Merge 2 times and be centrifuged obtained precipitating, 55 DEG C, 0.01MPa, after 18 hours dry, is crushed up to protein after the recovery.It adopts
With the protein concentration of biuret method detection Yu Porridge rinsing liquid and supernatant, it is computed and show that protein recovery is 90.6%.
Embodiment 3:
By hairtail decaptitating, tail and internal organ, meat is adopted by hand, is rubbed, is weighed flesh of fish 3kg, with 4 DEG C, 0.3%NaCl solution conduct
Rinsing liquid, the ratio of the control flesh of fish and rinsing liquid is 1:3 when rinsing, i.e., the 3kg flesh of fish is added in 9kg rinsing liquid, first at a slow speed
Stirring 10 minutes, then stand after twenty minutes with four layers of filtered through gauze, rinsing 2 times is repeated, Yu Porridge rinsing liquid is obtained；It is rinsed Xiang Yu Porridge
2.5g/L chitosan (with peracetic acid formulation at solution, concentration 1%) and 0.075g/L cationic polyacrylamide are added in liquid
(being configured to solution, concentration 0.025% with water), stirs evenly, and is centrifuged after standing 90 minutes: 4000rpm, 4 DEG C, 15 points
Clock takes supernatant and collects precipitating；Into supernatant be added 0.06g/L anionic polyacrylamide (be configured to solution with water, it is dense
0.025%) degree is, stirs evenly, be centrifuged after standing 120 minutes: 4000,3 DEG C, 15 minutes take supernatant and collect and sink
It forms sediment；Merge 2 times and be centrifuged obtained precipitating, 60 DEG C, 0.09MPa, after 22 hours dry, is crushed up to albumen after the recovery
Matter.Using the protein concentration of biuret method detection Yu Porridge rinsing liquid and supernatant, it is computed and show that protein recovery is
92.0%.
Embodiment 4:
By hairtail decaptitating, tail and internal organ, meat is adopted by hand, is rubbed, is weighed flesh of fish 4kg, with 4 DEG C, 0.3%NaCl solution conduct
Rinsing liquid, the ratio of the control flesh of fish and rinsing liquid is 1:3 when rinsing, i.e., the 4kg flesh of fish is added in 12kg rinsing liquid, first at a slow speed
Stirring 10 minutes, then stand after twenty minutes with four layers of filtered through gauze, rinsing 3 times is repeated, Yu Porridge rinsing liquid is obtained；It is rinsed Xiang Yu Porridge
3.0g/L chitosan (with peracetic acid formulation at solution, concentration 1%) and 0.08g/L cationic polyacrylamide (use are added in liquid
Water is configured to solution, concentration 0.025%), it stirs evenly, is centrifuged after standing 90 minutes: 4000rpm, 4 DEG C, 15 minutes,
It takes supernatant and collects precipitating；0.075g/L anionic polyacrylamide is added into supernatant and (is configured to solution, concentration with water
0.025%), to stir evenly, be centrifuged after standing 120 minutes: 4000rpm, 3 DEG C, 15 minutes take supernatant and collect and sink
It forms sediment；Merge 2 times and be centrifuged obtained precipitating, 60 DEG C, 0.09MPa, after 22 hours dry, is crushed up to albumen after the recovery
Matter.Using the protein concentration of biuret method detection Yu Porridge rinsing liquid and supernatant, it is computed and show that protein recovery is
95.0%.
The above embodiment of the present invention and data sufficiently confirm that protein recovery of the invention is high, can up to for 85~
95%, the COD (COD) with fish fish Porridge rinsing liquid is reduced, protection environment is of great significance.
Claims (7)
1. a kind of method for recycling protein from Dai Yu Yu Porridge rinsing liquid, it is characterised in that: add into Dai Yu Yu Porridge rinsing liquid
The composite flocculation agent that shell adding glycan and cationic polyacrylamide are constituted, uniformly mixed, centrifuging and taking supernatant simultaneously collect precipitating；So
The anionic polyacrylamide as flocculant is added in backward supernatant, uniformly mixed, centrifuging and taking supernatant simultaneously collects precipitating；
Precipitating twice merges, is dried to obtain protein；
Then the flesh of fish is added to work specifically the preparation method comprises the following steps: the hairtail flesh of fish is rubbed by the Dai Yu Yu Porridge rinsing liquid
First to mix slowly 5~15 minutes in 4 DEG C of rinsing liquid, 0.3%NaCl solution, then with multilayer gauze after standing 10~30 minutes
Filtering obtains fish Porridge rinsing liquid, and above-mentioned rinse cycle repeats 1~3 time, and the filtrate for merging repeatedly rinsing filtering obtains fish Porridge rinsing
Liquid；
Concentration of the chitosan and cationic polyacrylamide in Dai Yu Yu Porridge rinsing liquid is respectively 1.0g/L~3.0g/
L, the g/L of 0.02 g/L~0.08；
Concentration of the anionic polyacrylamide for being added to supernatant in supernatant is the g/L of 0.025 g/L~0.075.
2. the method according to claim 1 for recycling protein from Dai Yu Yu Porridge rinsing liquid, it is characterised in that: described
Chitosan is that the form for the chitosan solution for being first 1% at mass concentration with peracetic acid formulation is added in Dai Yu Yu Porridge rinsing liquid.
3. the method according to claim 1 for recycling protein from Dai Yu Yu Porridge rinsing liquid, it is characterised in that: described
Cationic polyacrylamide is first to be added with the form that water is configured to the cationic polyacrylamide solution that mass concentration is 0.025%
Enter into Dai Yu Yu Porridge rinsing liquid.
4. the method according to claim 3 for recycling protein from Dai Yu Yu Porridge rinsing liquid, it is characterised in that: described
Anionic polyacrylamide is that the shape for the anionic polyacrylamide solution that concentration of polymer solution is 0.025% is first configured to water
Formula is added in supernatant.
5. the method according to claim 1 for recycling protein from Dai Yu Yu Porridge rinsing liquid, it is characterised in that: described
Centrifugation, specifically are as follows: be centrifuged 10~15 minutes at 3500~4000rpm, 1 ~ 5 DEG C, then take supernatant and collect precipitating.
6. the method according to claim 1 for recycling protein from Dai Yu Yu Porridge rinsing liquid, it is characterised in that: described
It is dry, specifically by precipitating be placed in 50~60 DEG C, under the conditions of 0.08~0.1MPa it is 12~24 hours dry, then through crushing to obtain the final product
Protein after the recovery.
7. the method according to any claim from 1 to 6 that protein is recycled from Dai Yu Yu Porridge rinsing liquid,
Be characterized in that: specific step includes:
(1) the hairtail flesh of fish is rubbed and weighs weight；With 4 DEG C, 0.3% NaCl solution as rinsing liquid, when rinsing the control flesh of fish and
The weight ratio of NaCl solution is 1:3 ~ 5, the flesh of fish is added in above-mentioned NaCl solution, is first mixed slowly 5~15 minutes, then quiet
Multilayer filtered through gauze is used after setting 10~30 minutes, is repeated rinsing 1~3 time, is obtained fish Porridge rinsing liquid；
(2) it is prepared to step (1) and 1.0g/L~3.0g/L chitosan and 0.02 g/L~0.08 is added in Yu Porridge rinsing liquid
G/L cationic polyacrylamide, stirs evenly, and is then allowed to stand 30~120 minutes and obtains mixture；
(3) step (2) resulting mixture is centrifuged, 3500~4000rpm, 1 ~ 5 DEG C, 10~15 minutes take supernatant
And collect precipitating；
(4) the g/L anionic polyacrylamide of 0.025 g/L~0.075 is added into step (3) resulting supernatant, stirs
Uniformly, it is then allowed to stand 30~120 minutes and obtains mixture；
(5) step (4) resulting mixture is centrifuged, 3500~4000rpm, 1 ~ 5 DEG C, 10~15 minutes take supernatant
And collect precipitating；
(6) precipitating that collection step (3) and step (5) obtain, 50~60 DEG C, 0.08~0.1MPa, after 12~24 hours dry,
It is crushed up to protein after the recovery.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611167844.0A CN106800584B (en) | 2016-12-16 | 2016-12-16 | The method of protein is recycled from Dai Yu Yu Porridge rinsing liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611167844.0A CN106800584B (en) | 2016-12-16 | 2016-12-16 | The method of protein is recycled from Dai Yu Yu Porridge rinsing liquid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106800584A CN106800584A (en) | 2017-06-06 |
| CN106800584B true CN106800584B (en) | 2019-10-25 |
Family
ID=58984567
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611167844.0A Active CN106800584B (en) | 2016-12-16 | 2016-12-16 | The method of protein is recycled from Dai Yu Yu Porridge rinsing liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106800584B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110526469A (en) * | 2019-09-10 | 2019-12-03 | 河北首朗新能源科技有限公司 | A kind of processing of fermentation waste water and mycoprotein recovery process and system |
| CN112679593B (en) * | 2020-12-28 | 2023-01-03 | 华中农业大学 | Method for recovering sarcoplasmic proteins in minced fillet rinsing liquid by combining acid treatment with chitosan flocculation and application |
| CN112778396A (en) * | 2020-12-31 | 2021-05-11 | 福建省水产研究所（福建水产病害防治中心） | Method for recovering water-soluble protein in minced fillet |
| CN116178483A (en) * | 2022-12-13 | 2023-05-30 | 江苏大学 | Method for recycling protein in surimi rinsing waste liquid based on low-temperature plasma technology |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1763000A (en) * | 2005-09-23 | 2006-04-26 | 中国科学院海洋研究所 | From chitin, chitosan factory effluent, reclaim astaxanthin and method of protein |
| CN101624230A (en) * | 2008-07-11 | 2010-01-13 | 烟台海岸带可持续发展研究所 | Method of processing waste water and recycling protein |
| CN102747125A (en) * | 2012-06-25 | 2012-10-24 | 宁波武盛化学有限公司 | Preparation method of antioxidative peptide of hairtail |
| CN102757491A (en) * | 2012-07-11 | 2012-10-31 | 集美大学 | Method for recycling water-soluble proteins in minced fish rinsing water |
| CN103864911A (en) * | 2014-03-20 | 2014-06-18 | 上海海洋大学 | Method for recovering water-soluble protein in minced fillet rinse water by using composite flocculant |
-
2016
- 2016-12-16 CN CN201611167844.0A patent/CN106800584B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1763000A (en) * | 2005-09-23 | 2006-04-26 | 中国科学院海洋研究所 | From chitin, chitosan factory effluent, reclaim astaxanthin and method of protein |
| CN101624230A (en) * | 2008-07-11 | 2010-01-13 | 烟台海岸带可持续发展研究所 | Method of processing waste water and recycling protein |
| CN102747125A (en) * | 2012-06-25 | 2012-10-24 | 宁波武盛化学有限公司 | Preparation method of antioxidative peptide of hairtail |
| CN102757491A (en) * | 2012-07-11 | 2012-10-31 | 集美大学 | Method for recycling water-soluble proteins in minced fish rinsing water |
| CN103864911A (en) * | 2014-03-20 | 2014-06-18 | 上海海洋大学 | Method for recovering water-soluble protein in minced fillet rinse water by using composite flocculant |
Non-Patent Citations (1)
| Title |
|---|
| 《海产品加工废水蛋白质回收的研究》;方志民等;《浙江工业大学学报》;20080430;第36卷(第2期);第133-135页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106800584A (en) | 2017-06-06 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106800584B (en) | The method of protein is recycled from Dai Yu Yu Porridge rinsing liquid | |
| CN1294832C (en) | High efficiency protein extraction | |
| CN1098644C (en) | Stabilizer for meat products and meat product composition | |
| CN100351273C (en) | Heterogeneous carrageenan manufacturing process from mono component seaweed with reduced use of level of koh | |
| CN105439270B (en) | A kind of Moringa oleifera flocculant particle and its production and use | |
| CN102273665B (en) | Preparation method of shrimp shell ultrafine powder | |
| CN104126807A (en) | Method for continuously producing composite amino acid short peptide chelated calcium and chitin by using waste catering shrimp shells | |
| CN1297691A (en) | Separation and extraction process of effective component in resource insect | |
| CN106244659A (en) | The method of preparation poultry Cartilage collagen polypeptide | |
| CN109601898A (en) | A method of improving minced fish gel intensity | |
| CN105451570A (en) | Method for collecting proteins from waste water discharged during process for manufacturing fish meat processed food | |
| CN105348411A (en) | Method for preparing beta-chitin from sector bone of squid | |
| CN105084501B (en) | Biological flocculation aid and preparation method thereof is prepared using plant Chinese ilex | |
| CA1095772A (en) | Process for producing surimi | |
| CA1119879A (en) | Method of producing minced fish meat | |
| CN107311188B (en) | The modified method for preparing nano carrier material of attapulgite depth | |
| CN108613957A (en) | A kind of detection method for micro- plastics in aquatic food | |
| CN102726599B (en) | Preparation process for novel bone meal | |
| KR0185532B1 (en) | Preparation process of jelly type products using alginic acid from seaweed | |
| CN109370446B (en) | Method for clarifying gelatin solution by comprehensively utilizing waste residues | |
| CN102965030A (en) | Preparation method of high-gel-strength fish scale gelatin | |
| CN1049565C (en) | Conservation of orthophosphate waste | |
| CN105036232A (en) | Novel algaecide for lakes and preparation method thereof | |
| RU2296721C1 (en) | Method of purification of the industrial low-concentration waste waters from the substances of the protein-lipidic nature | |
| CN109052602A (en) | A kind of natural macromolecule flocculating agent and its processing method for starch wastewater treatment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |