CN102961456A - Preparation method and application of tsinling larkspur root extractive - Google Patents
Preparation method and application of tsinling larkspur root extractive Download PDFInfo
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- CN102961456A CN102961456A CN2012104331362A CN201210433136A CN102961456A CN 102961456 A CN102961456 A CN 102961456A CN 2012104331362 A CN2012104331362 A CN 2012104331362A CN 201210433136 A CN201210433136 A CN 201210433136A CN 102961456 A CN102961456 A CN 102961456A
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Abstract
The invention discloses a preparation method and application of a tsinling larkspur root extractive. The preparation method comprises the following steps of: (1) weighing crushed tsinling larkspur root, adding 95% of ethanol, and dipping for 0.5 hour; (2) carrying out reflux extraction for 3 hours at 60 DEG C in three times by a soxhlet extractor, and repeating the step three times; and (3) concentrating the extracting solution obtained in step (2) by a rotary evaporator until the extracting solution is nearly dry, so as to obtain the extractive, and then dissolving by DMSO (Dimethylsulfoxide). The preparation method of the tsinling larkspur root extractive is simple; and the tsinling larkspur root extractive is low in price and has a certain antineoplastic effect, so that the tsinling larkspur root extractive has a great application value for preparation of antineoplastic drugs.
Description
Technical field
The invention belongs to medical technical field, relate to a kind of preparation method and application thereof of Radix Delphinii Giraldii extract.
Background technology
Tumor is one of principal disease that threatens at present human health, and chemotherapy then is a kind of main method of present oncotherapy.Tradition synthetic chemistry cancer therapy drug is expensive and toxic and side effects is large, restricts its application.Chinese traditional herbs has many target spots when playing a role, specificity is strong and the less characteristics of toxic and side effects.Chinese herbal medicine and the research and development of active component cancer therapy drug thereof are because its huge applications is worth and market prospect becomes study hotspot both domestic and external.
Ranuculaceae (Delphinium giraldii) is perennial herb, is distributed in the ground such as Shaanxi, Henan, Gansu, Sichuan, Shanxi, Hubei, Ningxia, grows in the area of 960 meters to 2000 meters of height above sea level, is grown in mountain region Cao Po and the woods more.Radix Delphinii Giraldii is the root of ranunculaceae plant Ranuculaceae, and is hard, is crineous.Excavate autumn, removes stem and leaf and fibrous root, only stays root, cleans earth, dries and namely get Radix Delphinii Giraldii." national Chinese herbal medicine compilation " record: warm in nature, invigorate blood circulation pain relieving.Cure mainly headache, lumbago and backache, stomachache, and Radix Delphinii Giraldii extract antitumor action has no report.
Summary of the invention
In order to address the above problem, the invention provides a kind of preparation method and application thereof of Radix Delphinii Giraldii extract, the new purposes of Radix Delphinii Giraldii extract is the application of Radix Delphinii Giraldii extract in antitumor drug.The preparation method of this Radix Delphinii Giraldii extract is easy and simple to handle, is easy to promote.
Its technical scheme is as follows:
A kind of preparation method of Radix Delphinii Giraldii extract may further comprise the steps:
(1) Radix Delphinii Giraldii that takes by weighing through pulverizing adds 95% ethanol, soaks 0.5h;
(2) under 60 ℃, adopt apparatus,Soxhlet's reflux, extract, 3h, repeat 3 times;
(3) extracting solution that step (2) is obtained obtains extract with concentrated near the doing of Rotary Evaporators, dissolves with DMSO.
The application of Radix Delphinii Giraldii extract of the present invention in antitumor drug.
Beneficial effect of the present invention:
The preparation method of Radix Delphinii Giraldii extract of the present invention is simple, low price, and have certain antitumous effect, so the Radix Delphinii Giraldii extract of the present invention's preparation has important using value to the preparation of antitumor drug.
The specific embodiment
Below in conjunction with the specific embodiment technical scheme of the present invention is described in more detail.
(1) takes by weighing Radix Delphinii Giraldii 500g through pulverizing, add 2.5L95% ethanol and mix, soak 0.5h;
(2) under 60 ℃, adopt apparatus,Soxhlet's reflux, extract, 3h, repeat 3 times;
(3) extracting solution is concentrated near dried with Rotary Evaporators, obtain 5.0 gram extracts, dissolve with DMSO.
Collect In vitro culture hepatocarcinoma (HepG-2), gastric cancer (MGC-803) and pulmonary carcinoma (A-549) cell of exponential phase, transfer density to 1 * 10
5Cells/mL is inoculated in 96 orifice plates with 200 μ L/well, puts 37 ℃, 5%CO
2After cultivating 24h in the incubator of saturated steam, be that the amount of 0.05,0.5 and 5 μ g/mL adds in the culture fluid with the Radix Delphinii Giraldii extract according to final concentration, adopt mtt assay to detect the activity of tumor cells situation.The result shows the activity that can significantly suppress HepG-2, MGC-803 and A-549 when the concentration of Radix Delphinii Giraldii is 5 μ g/mL.
Table 1 Radix Delphinii Giraldii extract is on the impact (meansigma methods ± standard deviation) of activity of tumor cells
*Expression p＜0.05;
*Expression p＜0.01
From the above results as can be known, the Radix Delphinii Giraldii extract can suppress the activity of kinds of tumor cells, points out its application that can be used for antitumor drug, especially the cancer therapy drug of hepatocarcinoma, gastric cancer and pulmonary carcinoma is developed.
The above; it only is best mode for carrying out the invention; Radix Delphinii Giraldii extract of the present invention plays a role too in the exploitation of the cancer therapy drug method of other cancer; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses, the simple change of the technical scheme that can obtain apparently or equivalence are replaced and are all fallen within the scope of protection of the present invention.
Claims (2)
1. the preparation method of a Radix Delphinii Giraldii extract is characterized in that, may further comprise the steps:
(1) Radix Delphinii Giraldii that takes by weighing through pulverizing adds 95% ethanol, soaks 0.5h;
(2) under 60 ℃, adopt apparatus,Soxhlet's reflux, extract, 3h, repeat 3 times;
(3) extracting solution that step (2) is obtained obtains extract with concentrated near the doing of Rotary Evaporators, dissolves with DMSO.
2. the application of the described Radix Delphinii Giraldii extract of claim 1 in antitumor drug.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104331362A CN102961456A (en) | 2012-10-26 | 2012-10-26 | Preparation method and application of tsinling larkspur root extractive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012104331362A CN102961456A (en) | 2012-10-26 | 2012-10-26 | Preparation method and application of tsinling larkspur root extractive |
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| CN102961456A true CN102961456A (en) | 2013-03-13 |
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| CN2012104331362A Pending CN102961456A (en) | 2012-10-26 | 2012-10-26 | Preparation method and application of tsinling larkspur root extractive |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105125652A (en) * | 2015-10-12 | 2015-12-09 | 刘高志 | Tsinling larkspur root extract and preparing method and medical application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06247830A (en) * | 1993-02-24 | 1994-09-06 | Tokyo Tanabe Co Ltd | New hair growth agent |
| CN102274302A (en) * | 2011-06-07 | 2011-12-14 | 南京泽朗农业发展有限公司 | Preparation method of delphinium total diterpenoid alkaloids |
-
2012
- 2012-10-26 CN CN2012104331362A patent/CN102961456A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06247830A (en) * | 1993-02-24 | 1994-09-06 | Tokyo Tanabe Co Ltd | New hair growth agent |
| CN102274302A (en) * | 2011-06-07 | 2011-12-14 | 南京泽朗农业发展有限公司 | Preparation method of delphinium total diterpenoid alkaloids |
Non-Patent Citations (2)
| Title |
|---|
| XIAN-LI ZHOU，ET AL: "Three New C19-Diterpenoid Alkaloids from Delphinium giraldii", 《CHEM. PHARM. BULL》 * |
| 王莉: "九连环中生物碱的提取工艺研究", 《东华理工大学学报( 自然科学版)》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105125652A (en) * | 2015-10-12 | 2015-12-09 | 刘高志 | Tsinling larkspur root extract and preparing method and medical application thereof |
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Application publication date: 20130313 |