CN102960375A - Application of barbary wolfberry fruit ethanol extract - Google Patents
Application of barbary wolfberry fruit ethanol extract Download PDFInfo
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- CN102960375A CN102960375A CN2012105499337A CN201210549933A CN102960375A CN 102960375 A CN102960375 A CN 102960375A CN 2012105499337 A CN2012105499337 A CN 2012105499337A CN 201210549933 A CN201210549933 A CN 201210549933A CN 102960375 A CN102960375 A CN 102960375A
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- ethanol extract
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- chinese wolfberry
- wolfberry fruit
- mother liquor
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Abstract
The invention discloses an application of a barbary wolfberry fruit ethanol extract on the aspect of pesticides, and particularly relates to an application of the barbary wolfberry fruit ethanol extract on the aspect of killing cotton bollworms and resisting plant early blight. The barbary wolfberry fruit ethanol extract disclosed by the invention can effectively kill the cotton bollworms and inhibit the pathogens of the plant early blight, thereby enlarging the selection range of biological pesticides.
Description
Technical field
The present invention relates to a kind of new purposes of fruit of Chinese wolfberry ethanol extract, be specifically related to the application of fruit of Chinese wolfberry ethanol extract aspect agricultural chemicals.
Background technology
Cotton bollworm (Heliothis armigera) belongs to the Lepidoptera Noctuidae, and its host range is wide, and fertility is strong, is one of primary pest of Asia, Oceania, Africa and European various places harm cotton and other crops, is global crop pests.Since the nineties, cotton bollworm obviously increases in main cotton producing region outbreak year frequencies such as China the Yellow River, the Changjiang river, Liaohe River Basin and Xinjiang, causes cotton underproduction rate up to 35～50%, part field even total crop failure that some is regional from eighties of last century.
Early epidemic germ (Alternaria solani) is the pathogen that causes plant of Solanaceae early blight, the vegetables such as tomato, potato, capsicum, eggplant of mainly causing harm.How in the morbidity in seedling stage, blade tip or terminal bud produce the water stain shape scab of crineous, cause rottenly, and feel is smooth, after rotting in seedling top, form without the top seedling, in addition mashed to the native face of bed, cause seedling stage just dead, endanger very large.
In order to prevent and treat disease, people increase resistance by breeding resistant variety on the one hand, carry out on the one hand chemical control.The former has certain limitation, and the latter can bring environmental pollution.Therefore the novel agrochemical of efficient, the low toxicity of exploitation, low-residual especially biogenic pesticide more and more come into one's own.
The fruit of Chinese wolfberry is the dry mature fruit of plant of Solanaceae lycium barbarum Lycium barbarum L., and the fruit of congener L.Chinense Mill. is practised and claimed " river matrimony vine " or " Feng matrimony vine ", make the fruit of Chinese wolfberry in the minority area and use, but quality is inferior.The fruit of Chinese wolfberry is traditional conventional Chinese medicine simply, begins to be stated from Shennong's Herbal, classifies as top grade.Compendium of Material Medica is called its kidney tonifying, moistening lung, production of sperm, beneficial gas.Holy Benevolent Prescriptions carries wolfberry fruit wine qi-restoratives damage, strengthening the bones and muscles, pleases color, body-building body.The fruit of Chinese wolfberry is flat, flavor is sweet, have nourishing liver and kidney, the effect of benefiting shrewd head.Modern pharmacology studies show that, the fruit of Chinese wolfberry has antibacterial (human body pathogen), anti-mutagenesis, reducing blood lipid, hypoglycemic, anti-ageing, the antagonism to plumbous immunotoxicity, immunoregulation effect.But its application aspect agricultural chemicals is not arranged.
Summary of the invention
In order to develop new biogenic pesticide, the invention provides the purposes of fruit of Chinese wolfberry ethanol extract aspect agricultural chemicals, relate generally to poisoning cotton bollworm (Heliothis armigera) and anti-plant early blight (Alternaria solani).
Fruit of Chinese wolfberry ethanol extract at first adopts the DMSO dissolving to be prepared into mother liquor when using.
When being applied in anti-early blight pathogen, be 20PPM with above-mentioned mother liquor with the concentration that 0.025% polysorbas20 is diluted to fruit of Chinese wolfberry ethanol extract.
Being applied in poisoning during cotton bollworm, is 60 μ g/cm with above-mentioned mother liquor with the concentration that 0.025% polysorbas20 is diluted to fruit of Chinese wolfberry ethanol extract
2
The present invention has the following advantages compared to existing technology: utilize fruit of Chinese wolfberry ethanol extract as desinsection, antibacterial agent, effectively desinsection, Suppressing phytopathogens, its wide material sources are nontoxic to people and animals, can not produce and produce " three R " in the chemical pesticide use procedure, to the less pollution of environment.
Embodiment
Below in conjunction with specific embodiment fruit of Chinese wolfberry ethanol extract of the present invention is elaborated in the application aspect the agricultural chemicals.
Test sample: fruit of Chinese wolfberry medicinal material is purchased from Nanjing medicine company limited company.
Experimental instrument: high speed Universalpulverizer; Vertical automatic pressure steam sterilizer; Shaking table; The ultrasonic oscillation instrument; Rotary Evaporators; Thermostat water bath; Electronic balance; The intelligence climatic cabinate; Vavuum pump.
Extracting method: the fruit of Chinese wolfberry filters after 2 hours with (60～100%) ethanol 1: 8～10 (W/V) refluxing extraction, filter residue filters after 2 hours with the extraction of 8 times of amounts (60～100%) alcohol reflux again, and filtrate merges, reduced pressure concentration, 60 ℃ lower dry, gets brown medicinal extract.
Mother liquor configuration: get fruit of Chinese wolfberry ethanol extract (brown medicinal extract) with the DMSO dissolving, be prepared into the mother liquor that concentration is 10mg/mL.
The configuration of test solution: during use mother liquor is diluted to debita spissitudo with 0.025%Tween 20.
Embodiment one
For trying insect: cotton bollworm (Heliothis armigera), diamond-back moth (Plutella xylostella) and bean aphid (Aphis craccivora) are provided by the academy of agricultural sciences, Hubei Province.
Test method: during take diamond-back moth and cotton bollworm as the examination worm, ovum adopts the feed formula through academy of agricultural sciences, improved Beijing beam leather plum, the indoors artificial raising with 10% formalin sterilization 15min.The raising condition: 25 ℃ of temperature, relative moisture are 75%-85%.Sample Dilution is coated the artificial feed surface to finite concentration.Adopt 96 porocyte culture plates to screen.Every hole adds 300 μ L artificial feed and sample diluting liquid 20 μ L, checking experiment result after the week.Sample concentration is 1.0mg/mL during Preliminary screening, sieves again concentration and adjusts according to the primary dcreening operation result.
Adopt infusion method during take bean aphid as the examination worm.Primary dcreening operation concentration is 1.0mg/mL, directly has the bean seedlings of bean aphid to be immersed in the liquid with long, check result after the week.Sieving again concentration adjusts according to the primary dcreening operation result.
Result of the test: fruit of Chinese wolfberry ethanol extract is 60 μ g/cm in the sample ultimate density
2The time, cotton bollworm is had 100% toxic action, to diamond-back moth and bean aphid without the poisoning effect.
Embodiment two
For trying bacterium: Pythium ultimum bacterium (Pythium ultimum), botrytis cinerea (Botrytis cinerea), the former bacterium of wheat glume blight (Septoria nodorum), early blight pathogen (Alternaria Solani), gibberella saubinetii cause of disease bacterium (Fusarium culmorum), the former bacterium of damping off (Rhizoctonia solani) are provided by the academy of agricultural sciences, Hubei Province.
Test method: semisolid culturemedium method.Adopt 96 porocyte culture plates to screen.Every hole adds the mycelium of 90 μ L medium and fungi to be measured or the mixture of spore, and it is 10 μ L that every hole adds amount of liquid medicine.Primary dcreening operation sample final concentration in medium is respectively 20ppm.Sieving again concentration adjusts according to the primary dcreening operation result.
Result of the test: test shows that fruit of Chinese wolfberry ethanol extract has stronger inhibitory action to the early blight pathogen when the sample ultimate density is 20ppm, and inhibiting rate is 77%.
Claims (5)
1. the purposes of fruit of Chinese wolfberry ethanol extract aspect agricultural chemicals.
2. purposes according to claim 1 is characterized in that: described fruit of Chinese wolfberry ethanol extract is used for the poisoning cotton bollworm.
3. purposes according to claim 2, it is characterized in that: described fruit of Chinese wolfberry ethanol extract is when using, adopt first DMSO dissolving preparation mother liquor, getting described mother liquor before the use is 60 μ g/cm with the application concentration that 0.025% polysorbas20 is diluted to fruit of Chinese wolfberry ethanol extract
2
4. purposes according to claim 1 is characterized in that: described fruit of Chinese wolfberry ethanol extract is used for suppressing plant early blight pathogen, control plant of Solanaceae early blight.
5. purposes according to claim 4, it is characterized in that: described fruit of Chinese wolfberry ethanol extract is when using, adopt first DMSO dissolving preparation mother liquor, get described mother liquor before using and be 20ppm with the concentration that 0.025% polysorbas20 is diluted to fruit of Chinese wolfberry ethanol extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105499337A CN102960375A (en) | 2012-12-18 | 2012-12-18 | Application of barbary wolfberry fruit ethanol extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105499337A CN102960375A (en) | 2012-12-18 | 2012-12-18 | Application of barbary wolfberry fruit ethanol extract |
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| CN102960375A true CN102960375A (en) | 2013-03-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN2012105499337A Pending CN102960375A (en) | 2012-12-18 | 2012-12-18 | Application of barbary wolfberry fruit ethanol extract |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007139382A2 (en) * | 2006-05-29 | 2007-12-06 | Praktijkonderzoek Plant & Omgeving B.V. | Method for controlling a fungal foliar pathogens with an extract from yucca |
| CN102755280A (en) * | 2012-05-22 | 2012-10-31 | 张旺凡 | Chinese herbal medicine (compound) shampoo and preparation method thereof |
-
2012
- 2012-12-18 CN CN2012105499337A patent/CN102960375A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007139382A2 (en) * | 2006-05-29 | 2007-12-06 | Praktijkonderzoek Plant & Omgeving B.V. | Method for controlling a fungal foliar pathogens with an extract from yucca |
| CN102755280A (en) * | 2012-05-22 | 2012-10-31 | 张旺凡 | Chinese herbal medicine (compound) shampoo and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| 刘海伟: "菊芋叶片提取物杀虫抑菌活性的研究", 《南京农业大学硕士学位论文》, 6 March 2008 (2008-03-06) * |
| 王汉卿 等: "超声提取枸杞叶中总黄酮提取工艺及其不同采收期含量变化研究", 《中国实验方剂学杂志》, vol. 17, no. 8, 30 April 2011 (2011-04-30), pages 44 - 47 * |
| 田菲菲 等: "植物提取物对黄瓜炭疽病菌的抑制作用", 《中国农学通报》, vol. 22, no. 12, 31 December 2006 (2006-12-31), pages 327 - 330 * |
| 钱彦丛 等: "枸杞子的化学成分及药理研究新进展", 《中医药学报》, no. 4, 31 August 2000 (2000-08-31), pages 33 - 35 * |
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| PB01 | Publication | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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Application publication date: 20130313 |