CN102948568A - Coffee bean production method and method for preparing enzyme culture medium used in the same - Google Patents
Coffee bean production method and method for preparing enzyme culture medium used in the same Download PDFInfo
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- CN102948568A CN102948568A CN2011102356426A CN201110235642A CN102948568A CN 102948568 A CN102948568 A CN 102948568A CN 2011102356426 A CN2011102356426 A CN 2011102356426A CN 201110235642 A CN201110235642 A CN 201110235642A CN 102948568 A CN102948568 A CN 102948568A
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- coffee bean
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- enzyme
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Abstract
The invention relates to a coffee bean production method and a method for preparing an enzyme culture medium used in the production method. The method for preparing the enzyme culture medium used in the production method includes: at a temperature of 25-40DEG C, slicing the coffee beans excreted by civet cats fed with coffee beans, and soaking the sliced coffee beans in a high protein culture medium for 1-48h, with the high protein culture medium composed of a white fungus isolation nutrient solution and 15%-20% of water soluble protein; and acquiring the enzyme culture medium and other steps. The coffee bean production method consists of: at a temperature of 25-40DEG C, soaking shelled film-carrying coffee beans in the enzyme culture medium for 1-48h; taking out the soaked shelled film-carrying coffee beans; cleaning the soaked shelled film-carrying coffee beans; and acquiring a coffee bean product. By means of the high protein culture medium-bacterized enzyme culture medium, the sanitary and safety coffee bean product can be provided. Also, the coffee bean production method can maintain stable product quality and is easy for mass production.
Description
Technical field
The present invention relates to a kind of coffee bean manufacture method, particularly a kind of with the medium culture coffee bean so that the manufacture method of the coffee bean goods with flavour to be provided.
Background technology
Coffee bean has two-layer skin.Gather, shell, constant temperature cures, obtain the coffee bean through curing.Should after linsang drains, from the linsang excreta, take out coffee bean through the roast coffee beans feeding to linsang, and toast after it is cleaned, thereby obtain a kind of existing coffee bean goods.
Yet these existing coffee bean goods have following shortcoming at least:
1, these existing coffee bean goods take out from the linsang excreta, the manufacture method of these existing coffee bean goods is except failing to guarantee to clean in the clean situation, to cause outside the pathogen such as the residual excreta of being infected with of coffee bean and bacterium, this association also will cause psychological sense of discomfort for the consumer.
2, these existing coffee bean goods relate to the effect of organism, and the digestion of linsang, Excretion.And organism may be inadaptable etc. former thereby can not go out such as expection planning real estate the coffee bean of quality, stable yield because of sick or Rearing Condition, and therefore the manufacture method of these existing coffee bean goods is difficult to properly supply coml and stablizes supply of material demand.
3, these existing coffee bean goods are essential through the feeding linsang, treat its drainage, from excreta, take out again coffee bean and make existing coffee bean goods, because defecation next day of linsang, need wait for 48 hours from feeding linsang coffee bean approximately to its drainage, not only manufacturing time is long, and few corresponding to the output of single linsang, and the manufacture method of these existing coffee bean goods can't properly be supplied commercial mass-produced demand as can be known.
Summary of the invention
Because the shortcoming of existing coffee bean product and making method the object of the present invention is to provide a kind of coffee bean manufacture method for the manufacture of the coffee bean goods.Coffee bean manufacture method provided by the present invention can be for the manufacture of safe and sanitary and the stable coffee bean goods of quality and yield, and are easy to improve output and produce in a large number.
The manufacture method of the enzyme culture medium for the coffee bean manufacture method provided by the present invention includes:
Under 25 to 40 degree Celsius, to take from by after the coffee bean that linsang drained of the feeding coffee bean section and be soaked in a high protein culture medium 1 to 48 hour, this high protein culture medium includes tremella to be separated nutrient solution and contains 15% to 20% water soluble protein;
Obtain an enzyme culture medium.
The invention still further relates to a kind of coffee bean manufacture method, include:
Under 25 to 40 degree Celsius, the coffee bean of the film that shells is soaked in aforementioned enzyme culture medium 1 to 48 hour;
The coffee bean of the shell film of taking-up through soaking;
The coffee bean of the shell film of cleaning through soaking;
Obtain the coffee bean goods.
Be soaked in a high protein culture medium by taking from by the section of the coffee bean that linsang drained of feeding coffee bean, this high protein culture medium bacterium can be turned to the enzyme culture medium.In coffee bean manufacture method provided by the invention, use this enzyme culture medium, a kind of coffee bean goods of safe and sanitary can be provided.And because can keeping quality and yield, coffee bean manufacture method of the present invention stablizes, and being easy to improve output produces in a large number, in market, for should the coffee bean goods time, have the coml advantage, thereby overcome the shortcoming of existing coffee bean product and making method.
The specific embodiment
Embodiment 1
Enzyme culture medium for the coffee bean manufacture method of the present invention can adopt manufacture method provided by the present invention to make.The method of making this enzyme culture medium includes following steps:
1, under 25 to 40 degree Celsius, to take from by after the coffee bean that linsang drained of the feeding coffee bean section and be soaked in a high protein culture medium 1 to 48 hour, this high protein culture medium includes tremella to be separated nutrient solution and contains 15% to 20% water soluble protein.The tremella that adopts in the present embodiment is white fungus (Tremella fuciformis).In addition, adopt in the present embodiment the kjeldahl nitrogen analytic approach to measure water-soluble protein content.The kjeldahl nitrogen analytic approach mainly is to measure first nitrogen content, takes advantage of its coefficient to obtain protein content according to different materials again.
2, obtain an enzyme culture medium.
Wherein, aforementioned high protein culture medium can adopt a kind of culture medium that includes the compositions such as protein 18%, glucose 3%, agar 5% and distilled water 53%.In addition, aforementioned high protein culture medium can further include fruit extract, starch and coconut water.
Be soaked in this high protein culture medium by taking from by the section of the coffee bean that linsang drained of feeding coffee bean, can be so that this high protein culture medium be subject to taking from by the accompanying enzyme effect of the coffee bean that linsang drained of feeding coffee bean, and then allow this high protein culture medium be changed into an enzyme culture medium by bacterium.
When making aforementioned enzyme culture medium, this is being taken from when being soaked in this high protein culture medium by the coffee bean that linsang drained of feeding coffee bean, sustainably should be immersed with taking from by the high protein culture medium of the coffee bean that linsang drained of feeding coffee bean with per minute 30 times to the frequency oscillation of per minute 100 times, thus promote that bacterium turns usefulness into.
Embodiment 2
Coffee bean manufacture method of the present invention includes following steps:
1, under 25 to 40 degree Celsius, the coffee bean of the film that shells is soaked in aforementioned enzyme culture medium 24 to 48 hours.
2, take out the coffee bean of the film that shells through soaking.
3, clean the coffee bean of the film that shells through soaking.
4, obtain the coffee bean goods.These coffee bean goods contain the coffee bean of the aforementioned film that shells through cleaning.
In addition, after aforementioned the 3rd step, can further add a baking step.Namely after the coffee bean that cleans the film that shells through soaking, further dry this coffee bean through cleaning and obtain again the coffee bean goods.When this coffee bean through cleaning of oven dry, can make this drying course continue to obtain again at least 12 to 24 hours the coffee bean goods.
In addition, when the coffee bean with the aforementioned film that shells is soaked in the enzyme culture medium, sustainably with per minute 30 times to this immersion of frequency oscillation of per minute 100 times enzyme culture medium of coffee bean of film that shells, thereby strengthen the enzyme culture medium for the effect of the coffee bean of the aforementioned film that shells.
The enzyme culture medium that adopts aforementioned bacteriumization to form is processed the coffee bean of the film that shells, and can avoid directly taking from excreta coffee bean, and a kind of coffee bean goods of safe and sanitary can be provided.And because coffee bean manufacture method of the present invention is a kind of industrialized enzyme medium treatment manufacture method, wherein do not have zoic participation, needn't lean on biological agent unstable, consuming time and that be difficult to control, it is stable therefore can to keep quality and yield.Further, when the coffee bean goods that provide for coffee bean manufacture method of the present invention when market have wilderness demand, because this coffee bean manufacture method is a kind of industrialized enzyme medium treatment manufacture method, therefore can easily improve output by the mode that adds extensive (scale-up) and produce in a large number, in order to keep or compete the coml advantage in market.
Claims (5)
1. manufacture method that is used for the enzyme culture medium of coffee bean manufacture method includes:
Under 25 to 40 degree Celsius, to take from by after the coffee bean that linsang drained of the feeding coffee bean section and be soaked in a high protein culture medium 1 to 48 hour, this high protein culture medium includes tremella to be separated nutrient solution and contains 15% to 20% water soluble protein;
Obtain an enzyme culture medium.
2. the manufacture method of the enzyme culture medium for the coffee bean manufacture method according to claim 1, aforementioned high protein culture medium includes: protein 18%, glucose 3%, agar 5% and distilled water 53%.
3. the manufacture method of the enzyme culture medium for the coffee bean manufacture method according to claim 2, aforementioned high protein culture medium further includes fruit extract, starch and coconut water.
4. coffee bean manufacture method includes:
Under 25 to 40 degree Celsius, the coffee bean of the film that shells was soaked in according to claim 1 in 3 each described enzyme culture medium 1 to 48 hour;
The coffee bean of the shell film of taking-up through soaking;
The coffee bean of the shell film of cleaning through soaking;
Obtain the coffee bean goods.
5. coffee bean manufacture method according to claim 4 after the coffee bean that cleans the aforementioned film that shells through soaking, is further dried this coffee bean through cleaning and is obtained the coffee bean goods again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110235642.6A CN102948568B (en) | 2011-08-17 | 2011-08-17 | Coffee bean production method and method for preparing enzyme culture medium used in the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110235642.6A CN102948568B (en) | 2011-08-17 | 2011-08-17 | Coffee bean production method and method for preparing enzyme culture medium used in the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102948568A true CN102948568A (en) | 2013-03-06 |
| CN102948568B CN102948568B (en) | 2014-01-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110235642.6A Expired - Fee Related CN102948568B (en) | 2011-08-17 | 2011-08-17 | Coffee bean production method and method for preparing enzyme culture medium used in the same |
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| CN (1) | CN102948568B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090220645A1 (en) * | 2008-02-25 | 2009-09-03 | Luis Federico Martinez | Quality Enhancement of Coffee Beans by Acid and Enzyme Treatment |
-
2011
- 2011-08-17 CN CN201110235642.6A patent/CN102948568B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090220645A1 (en) * | 2008-02-25 | 2009-09-03 | Luis Federico Martinez | Quality Enhancement of Coffee Beans by Acid and Enzyme Treatment |
Non-Patent Citations (2)
| Title |
|---|
| MASSIMO F等: "A Composition and properties of Indonesian palm civet coffee", 《FOOD RESEARCH INTERNATIONAL》, vol. 37, no. 9, 31 December 2004 (2004-12-31), pages 901 - 912 * |
| 王琳娜: "最贵咖啡猫粪制造", 《经营者》, no. 11, 31 December 2008 (2008-12-31), pages 29 * |
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| Publication number | Publication date |
|---|---|
| CN102948568B (en) | 2014-01-01 |
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