CN102947690B - Automatic analysis device - Google Patents
Automatic analysis device Download PDFInfo
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- CN102947690B CN102947690B CN201180030416.2A CN201180030416A CN102947690B CN 102947690 B CN102947690 B CN 102947690B CN 201180030416 A CN201180030416 A CN 201180030416A CN 102947690 B CN102947690 B CN 102947690B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N2021/4704—Angular selective
- G01N2021/4711—Multiangle measurement
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/82—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
- G01N2021/825—Agglutination
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- General Physics & Mathematics (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Disclosed is an automatic analysis device provided with a light detector that detects scattered light, whereby highly reliable analysis results can be obtained by reduction of the impact of noise components. Highly reliable concentration analysis with little impact from noise components can be achieved by calculating the correlation between scattered light detected by a plurality of light detectors before calculating concentration, and by performing concentration analysis using scattered light with high correlation.
Description
Technical field
The present invention relates to the automatic analysing apparatus light by determination object scattering measured to determination object irradiation light, particularly relate to the automatic analysing apparatus of configuration two or more detecting device.
Background technology
As the analyzing device analyzed the component amount contained by sample (test portion, detection bodies), widely use following automatic analysing apparatus, namely, the light from light source is irradiated to sample or the reactant liquor that is mixed with sample and reagent, measuring through light quantity and calculating absorbance single or multiple wavelength that its result obtains, according to the rule of Lambert-Beer, calculate component amount from the relation of absorbance and concentration.
In these devices, in the tray of containers of repeatedly carrying out rotating and stop, circumferentially and show and keep multiple containers of reactant liquor, in tray of containers rotary course, utilize pre-configured through flash ranging bonding part, with about 10 minutes, certain time interval measures the rheological parameters' change with time of absorbance.
Automatic analysing apparatus possesses the system measured through light quantity, and on the other hand, in the reaction of reactant liquor, the general matrix and the color reaction of ferment and the congealing reaction these two kinds of antigen and antibody of using is reacted.
The former is biochemical analysis, has LDH(lactic dehydrogenase as inspection item), ALP(alkaline phosphatase), AST(glutamic-oxalacetic transaminease) etc.The latter is immunoassay, has CRP (c reactive protein), IgG(immunoglobulin (Ig) as inspection item), RF(rheumatoid factor) etc.
In the mensuration material that the immunoassay of the latter measures, blood level is low and require high sensitivity.In the past, achieve the high sensitive utilizing latex immunity coagulation as follows, namely, antibody is made to produce the reagent of allergic reaction (combination) to the surface of latex particle, identify composition contained in sample and make it condensation, now, to reactant liquor light projector, by to not in the scattering of latex coagula through light quantity measure, component amount contained in sample is carried out quantitatively.
In addition, as automatic analysing apparatus, also attempt not measuring the high-sensitivity utilizing through light quantity and measure amount of scattered light.Such as disclose: use barrier film and will be separated and the system of Simultaneously test absorbance and scattered light (patent documentation 1) with scattered light through light; By along with carrying out the result of congealing reaction and formation (patent documentation 2) that the specular scattering photo measure of the large coagula precision in high concentration side that formed improves; And before and after reaction vessel, use integrating sphere to measure the respective average light quantity of forward scattering light and backscattering light and to change to being offset the turbidity caused by container position the method (patent documentation 3) etc. revised.
Prior art document
Patent documentation
Patent documentation 1: Japanese Unexamined Patent Publication 2000-307117 publication
Patent documentation 2: Japanese Patent Application 2006-180338 publication
Patent documentation 3: the flat 9-153048 publication of Japanese Patent Application
Summary of the invention
Invent problem to be solved
In the automatic analysing apparatus employing the detecting device detecting scattered light, be mixed at determination object when being attached with scar in the situation of the foreign matters such as bubble, the damage of reaction vessel or reaction vessel, affect measurement result as noise contribution.
In order to reduce the impact of this noise, have by carrying out to the output carrying out self-detector the method that certain hour integration improves S/N ratio characteristic, but not only integral time determined object time change restriction, and when being attached with the foreign matters such as bubble in reaction vessel, the improvement effect of S/N ratio characteristic cannot be obtained.In addition, Patent Document 3 discloses following technology, that is, by scattered light integration and implement handling averagely, reduce S/N ratio.
But, irradiating light to measuring object in the light scattering photometer that detects the light of its scattering, principle is difficult to divide the bubble adhered in the scar of reaction vessel or reaction vessel and measuring object.
Further, the specific position attachment of the many meetings of bubble of attachment in reaction vessel in reaction vessel, and how the damage of reaction vessel also can at the privileged site of reaction vessel.Therefore, if the light of scattering on specific direction can be removed before concentration computing, then can use the light detecting signal of the few scattered light of noise contribution and obtain the high result of reliability.
Therefore, the object of the present invention is to provide following automatic analysing apparatus, even if the midway, path that the light of the noise that is, contained by scattered light, optical detection system passes through exists the obstacle passed through hindering scattered light, also can obtain the result of the high analysis of reliability.
For solving the method for problem
The invention is characterized in, from multi-direction, the amount of scattered light from determination object is measured, obtain measure direction mutual measure the related coefficient of amount of scattered light, and the amount of scattered light in the mensuration direction meeting the above-mentioned related coefficient higher than benchmark related coefficient is used for the analysis of said determination object.
The effect of invention
According to the present invention, due to the analysis using the high amount of scattered light of related coefficient to carry out determination object, thus the measurement result that reliability can be provided high to clinicing aspect.
Accompanying drawing explanation
Fig. 1 is the system chart that the entirety of the automatic analysing apparatus representing embodiments of the invention is formed.
Fig. 2 is embodiments of the invention, be System's composition figure to the optical detection system that the scattered light from determination object detects.
Fig. 3 is the measurement result of the scattered light detected by photodetector (θ 1, θ 2) of the optical detection system of embodiments of the invention.
Fig. 4 is embodiments of the invention, be the figure of the signal content of scattered light detected by optical detection system and the relation of noise contribution.
Fig. 5 is embodiments of the invention, is the correlogram being set to the scattered light detected by different multiple photodetectors (θ 1, θ 2) relative to the angle of inclination through optical axis.
Fig. 6 is embodiments of the invention, identical with Fig. 5, is the correlogram of the scattered light detected by multiple photodetector (θ 1, θ 3).
Fig. 7 is embodiments of the invention, identical with Fig. 5, is the correlogram of the scattered light detected by multiple photodetector (θ 1, θ 4).
Fig. 8 is embodiments of the invention, is represent the scattered light detected by multiple photodetector (θ 1, θ 2) is standardized and the figure of the light quantity of equalization.
Fig. 9 is embodiments of the invention, is represent the figure to the treatment scheme that the data of the scattered light detected by multiple photodetector process.
Figure 10 is embodiments of the invention, be the setting screen of the parameter of analysis shown by the display device of automatic analysing apparatus.
Embodiment
Below, with reference to the accompanying drawings embodiments of the invention are described.
Below, use Fig. 1 ~ Fig. 6, the formation of the automatic analysis system of an embodiment of the invention and action are described.
At first, use Fig. 1, the entirety of the automatic analysis system of present embodiment is formed and is described.Fig. 1 is the system chart that the entirety of the automatic analysis system representing embodiments of the present invention is formed.
Fig. 1 represents that the entirety of automatic analysing apparatus is formed.Be set to and in the reaction tray 1 of intermittent rotary, the multiple reaction vessels 2 be made up of translucent material can be circumferentially installed.Reaction vessel 2 is maintained the temperature (such as 37 DEG C) of regulation by calibration cell 3.Fluid in calibration cell 3 adjusts temperature by constant temperature keeping device 4.
Sample disc 5 loads multiple physical container 6, and the plurality of physical container 6 contains the Organism Samples of blood or urine and so on.The pipettor nozzle 8 being installed on moveable arm 7 sucks the sample of ormal weight from the physical container 6 of the suction position being positioned at sample disc 5, and is discharged by the reaction vessel 2 of its sample to the drain position be positioned in reaction tray 1.
Being configured in respectively on the reagent disc in reagent cold storage box 9A, 9B, load multiple reagent bottle 10A, 10B, the plurality of reagent bottle 10A, 10B post the label of the expression reagent identifying information of bar code and so on.Contain in these reagent bottles and obtain reagent solution corresponding to analysis project with by analytical equipment analysis.When registering reagent, each reagent cold storage box 9A, apparatus for reading of bar code 34A, 34B affiliated by 9B read the bar code that the outer wall at each reagent bottle represents.The reagent information read is registered into storer 11 described later together with the positional information on reagent disc.
The reagent pipettor nozzle of each reagent dispensing mechanism 12A, 12B accepts position from the reagent be positioned at reaction tray 1, the reagent bottle corresponding with inspection item sucks reagent solution, and discharges in suitable reaction vessel 2.
The potpourri being housed in sample in reaction vessel 2 and reagent is stirred mechanism 13A, 13B and stirs.Reaction vessel 2 arranges in rotary moving, to make by the light-metering position between light source 14 and light scattering photometer 15.Light scattering photometer 15 also can possess multi-wavelength extinction photometer on coaxial optical axis, also can use scattered light with through light both sides to carry out concentration computing.In addition, light source 14 and light scattering photometer 15 form optical detection system.The configuration of Fig. 2 to the photodetector in light scattering photometer 15 is used to be described below.
Then, in the spinning movement of reaction tray 1, whenever crossing before light scattering photometer 15, light-metering is carried out to the reactant liquor of the sample in each reaction vessel 2 and reagent.The simulating signal of the scattered light that each sample measures is inputted to A/D transducer 16.Be configured at the reaction vessel wiper mechanism 17 of the vicinity of reaction tray 1 by cleaning the inside of the reaction vessel 2 of finishing using, can Reusability reaction vessel.
Next, simply the control system of the analytical equipment of Fig. 1 and signal processing system are described.
Computing machine 18 is connected with sample dispensing control part 20, reagent dispensing control part 21, A/D transducer 16 via interface 19.Computing machine 18 pairs of sample dispensing control parts 20 send instruction, thus control the dispensing action of sample.In addition, computing machine 18 pairs of reagent dispensing control parts 21 send instruction, thus control the dispensing action of reagent.
The light value being transformed to digital signal by A/D transducer 16 is obtained by computing machine 18.
Interface 19 and the printer 22 for lettering, the storer 11 as memory storage or outside output medium 23, for the keyboard 24 to enter the operating instructions etc. and be connected for the CRT monitor (display device) 25 of display frame.As display device, liquid crystal display etc. can be adopted except CRT monitor.Storer 11 is such as made up of harddisk memory or external memory storage.In storer 11, store the information such as display level, analytical parameters, analysis project trust content, calibration result, analysis result of the password of each operator, each picture.
Next, the analysis action of the sample of the automatic analysing apparatus of Fig. 1 is described.
For the analytical parameters that the project can analyzed by automatic analysing apparatus is relevant, input via the message input device of keyboard 24 and so on and be stored in storer 11 in advance.Operator uses operating function picture described later to the inspection item selecting each sample to entrust.
Now, also the information such as patient ID are inputted from keyboard 24.In order to analyze the inspection item indicated relative to each sample, pipettor nozzle 8 according to analytical parameters from physical container 6 to the sample of reaction vessel 2 dispensing ormal weight.
The reaction vessel receiving sample is transferred due to the rotation of reaction tray 1, and accepts position stopping at reagent.The pipettor nozzle of reagent dispensing mechanism 12A, 12B according to the analytical parameters of suitable inspection item to the reagent solution of reaction vessel 2 dispensing ormal weight.Sample also can be contrary with this example with the dispensing order of reagent, dispensing sample after first dispensing reagent.
Afterwards, utilize rabbling mechanism 13A, 13B, carry out the stirring of sample and reagent, and make them mix.When this reaction vessel 2 crosses light-metering position, the scattered light of light scattering photometer 15 pairs of reactant liquors is utilized to carry out light-metering.Scattered light after light-metering is transformed to the numerical value proportional with light quantity by A/D transducer 16, and is obtained by computing machine 18 via interface 19.
Use the numerical value after this conversion, based on the typical curve that the analytic approach of being specified by each inspection item measures in advance, and be transformed to concentration data.Component concentration data as the analysis result of each inspection item exports at the picture of printer 22, CRT25.
Before performing above mensuration action, operator carries out analyzing the setting of various parameters required for measuring, the registration of test portion via the operation screen of CRT25.In addition, operator is confirmed the analysis result after mensuration by the operation screen on CRT25.
Next, use Fig. 2, the detailed formation of the light source 14 in Fig. 1 and light scattering photometer 15 is described.
Fig. 2 is the system chart representing that the entirety of light source/reaction vessel/light scattering photometer is formed.
Incident to the reaction vessel 202 point being marked with measuring object from the light of light source 201 incidence.Determination object comprises measuring object, reaction vessel 202.Light scattering photometer 15 is utilized to carry out light detection to the scattered light in determination object scattering.
Light scattering photometer 15 has 4 photodetectors (204,205,206,207).Photodetector uses photodiode.4 photodetectors (204,205,206,207) are configured to different relative to the degree of tilt through optical axis (angle 0 °) existed on the extended line of incident light axis.
The degree of tilt of photodetector 204 is θ 1.θ 1 such as can select 30 ° to 20 ° arbitrarily angled.The degree of tilt of photodetector 205 is θ 2, is the degree of tilt larger than θ 1.θ 2 and the differential seat angle of θ 1 such as can select 30 ° to 20 ° arbitrarily angled.
The degree of tilt of photodetector 206 is θ 3.θ 3 example is if the arbitrarily angled of – 30 ° to – 20 ° is selected in choosing.
The degree of tilt of photodetector 207 is θ 4.The degree of tilt of photodetector 207 is θ 4, is the degree of tilt larger than θ 3.θ 3 such as can select 30 ° to 20 ° arbitrarily angled.The configuration of multiple photodetector is the configuration that degree of tilt is different in the Z-axis direction relative to incident light axis, but also can configure with changing angle on X-axis, Y direction, vergence direction.Further, do not need to configure photodetector discretely, can configure photodetector continuously yet.
Then, incident light collides and scattering with measuring object in reaction vessel 202.The light of scattering is by photodetector 204(θ 1), photodetector 205(θ 2), photodetector 206(θ 3), photodetector 207(θ 1) detect.When carrying out this detection, when from reaction vessel to the path of photodetector midway such as have bubble, scar 203, can not be affected by relative to the scattered light being positioned at photodetector 207 light of the position of θ 4 through optical axis (0 °).
Next, the mensuration of the output signal shown in Fig. 3 is described.
This mensuration is to represent an example of the course of reaction of measuring object with the output signal of the scattered light detected by photodetector 204 ~ 205.The figure of the course of reaction represented by relation by measuring point (transverse axis), output signal (longitudinal axis).
That is, this course of reaction be the reaction vessel being circumferentially configured at reaction tray often with certain hour before photometer by time, through time ground repeatedly will detect that the situation of carrying out of the reaction that the mensuration of determination object starts till terminating is made curve.Herein, in the passage of time till measuring point 19 to measuring point 34 divides, course of reaction is represented by the output signal of photodetector (204,205).Measuring point, according to the serial number detected by photometer, increases the passing of time along with the increase of measuring point quantity.Passage of time divides can be selected within the scope of arbitrary measuring point, arbitrary division.
Generally, the light quantity of the scattered light large relative to the angle of inclination through optical axis is few, and in Fig. 3, photodetector 204(θ 1, angle of inclination are little) the light quantity of output valve many, photodetector 205(θ 2, angle of inclination are large) the light quantity of output valve few.
Next, the signal detected the photodetector shown in Fig. 4 and noise are described.
Fig. 4 is the component of signal (scattered light from original measuring object) of the component of signal schematically representing photodetector light and the figure of the relation of noise component (component of signal produced at random).
The semaphore of the scattered light of photodetector institute light is the semaphore that component of signal and noise component are added together.If component of signal such as represents with the numerical value shown in 100 ± α under desirable state, then the signal obtaining scattered light by any photodetector from the scattered light of identical measuring object should be all 100 ± α.On the other hand, noise component is owing to being subject to the impact of the signal of the scattered light of the light of photodetector at random thus can having positive and negative value.In addition, if photodetector is different, then can be subject to the impact of difference of its configuration, photodetector self.
Further, about component of signal, when have 203 shown in Fig. 3 and so on the bubble being attached to reaction vessel or scar etc., can change when specific direction hinders the key factor of some scattered light.
By the figure shown in Fig. 4 (1), Fig. 4 (2), numerical value can be clear and definite, and the S/N of photodetector (204,205) is than low, and the S/N of photodetector (206,207), than high, thinks that photodetector (206,207) is subject to the impact of noise many.
Next, the correlativity of the amount of scattered light that photodetector detects is described.
Fig. 5 represents photodetector 204(θ 1) with photodetector 205(θ 2) detected by the correlativity of amount of scattered light.By the output signal of the scattered light shown in Fig. 3 (photodetector 204(θ 1), photodetector 205(θ 2)) based on calculate correlativity.
According to photodetector 204(θ 1) with photodetector 205(θ 2) detected by the regression curve of amount of scattered light calculate 1 regression curve (straight line) with least square method.The formula of the expression correlationship of this 1 regression curve (straight line) is y=1.6776x – 0.5637.In addition, to photodetector 204(θ 1) with photodetector 205(θ 2) detected by amount of scattered light correlationship each other calculate, statistically calculate the known R as contribution rate
2, and the degree of tilt of regression straight line and intercept.Contribution rate R2 is equivalent to related coefficient.Degree of tilt and intercept are the coefficients of regression straight line.
Photodetector 204(θ 1) with photodetector 205(θ 2) detected by the related coefficient of amount of scattered light be 0.9929 and close to (1), represent high correlationship.
Fig. 6 represents photodetector 204(θ 1) with photodetector 206(θ 3) detected by the correlativity of amount of scattered light.Based on this photodetector 204(θ 1) with photodetector 206(θ 3) detected by the related coefficient (R of amount of scattered light
2) be 0.9314.Show photodetector 204(θ 1) with photodetector 206(θ 3) correlationship than photodetector 204(θ 1) with photodetector 205(θ 2) correlationship low.
Fig. 7 represents photodetector 204(θ 1) with photodetector 207(θ 4) detected by the correlativity of amount of scattered light.Based on this photodetector 204(θ 1) with photodetector 207(θ 4) detected by the related coefficient (R of amount of scattered light
2) be 0.8691.Show photodetector 204(θ 1) with photodetector 207(θ 4) correlationship than photodetector 204(θ 1) with photodetector 206(θ 3) correlationship lower.
Like this, photodetector 204(θ 1) with photodetector 207(θ 4) and photodetector 204(θ 1) with photodetector 206(θ 3) correlationship than photodetector 204(θ 1) with photodetector 205(θ 2) correlationship low, caused by the impact that the obstacles such as this is noise included by scattered light, the bubble that exists in the midway, path that the light of optical detection system passes through or scar bring.
Remove the scattered light that the impacts such as such noise, bubble or scar are high, select to affect on it photodetector 204(θ 1 that few scattered light detects) with photodetector 205(θ 2) detection signal and carry out the concentration analysis of sample, thus the result of the high concentration analysis of reliability can be obtained, and then the result of the concentration analysis that reliability can be provided high to clinicing aspect.
In addition, photodetector 204(θ 1) be the photodetector of benchmark observing in correlationship.Also can by photodetector 204(θ 1) beyond be chosen as the photodetector of benchmark, but preferably can carry out the detection of stable scattered light as the photodetector of benchmark, be difficult to produce the position configuration of the impacts such as noise, bubble or scar.Therefore, the photodetector of benchmark can carry out the stable detection of scattered light, and suitably can select not produce the angle of inclination of the impacts such as noise, bubble or scar and set.
Next, composition graphs 8, to photodetector 204(θ 1) with photodetector 205(θ 2) detected by the normalization of Output rusults (amount of scattered light) be described.
For the normalization of the Output rusults shown in Fig. 8, transverse axis represents measuring point, and the longitudinal axis represents equalization light quantity.The measuring point (transverse axis) of the measuring point shown in Fig. 8, equalization light quantity and above-mentioned Fig. 3, to output signal (longitudinal axis) corresponding.
In example shown in Fig. 8, make θ 1(photodetector 204) output valve in direction and θ 2(photodetector 205) direction the normalized situation of Output rusults under, use the coefficient of above-mentioned regression straight line (inclination/intercept) to make a side be normalized to the Output rusults of the opposing party (specific photodetector side).
In example shown in Fig. 8, become regression straight line, to 2 times, the formula of the correlationship of the data of amount of scattered light that obtains with multiple angle of 3 inferior expressions calculates and can standardize, can as arbitrary formula.Further, the opportunity obtained with photodetector detection scattered light is preferably repeatedly.
Like this, determine the typical curve of equalization light quantity in advance by irradiation and carry out concentration analysis, the analysis result that the better reliability of precision is higher can be obtained, wherein, equalization light quantity is the normalized amount of the amount of scattered light detected by multiple photodetectors making to have the high relation of correlationship.
Next, composition graphs 9, the treatment scheme of the data of the process scattered light illustrated using Fig. 1 ~ Fig. 8 is described.
First, the concentration analysis (step 301) of automatic analysing apparatus is started.Then, multiple photodetector (204,205,206,207) is utilized to obtain the different data of multiple angles of degree of tilt (detection of scattered light) (step 302).
In step 302, multiple photodetector (204,205,206,207) comprise light-metering point 19-34 on a large scale in the change being used as amount of scattered light is detected to the data of the concentration of the reactions change according to passage of time.Take out from this large-scale light-metering point and specify interval censored data (step 303).
The arbitrary division scope required for concentration analysis is selected to carry out the taking-up of this appointment interval censored data.Owing to can select the arbitrary measuring point of the reactions change according to passage of time within the scope of arbitrary division, thus can aptly and suitably implement concentration analysis.
Amount of scattered light detected by the multiple photodetectors selected in step 303 (204,205,206,207) obtains the related coefficient (step 304) of the data of high angle.For related coefficient (contribution rate, R
2), as mentioned above, from (1) more close to, correlationship is higher, from (0) more close to, correlationship is lower.
The data (step 305) that related coefficient is high are taken out based on the related coefficient of step 304.By this taking-up, select the amount of scattered light of the photodetector (204,205) that related coefficient is high from the amount of scattered light detected by multiple photodetector (204,205,206,207).The amount of scattered light that the benchmark related coefficient (threshold value) that removing related coefficient pre-enters than operating personnel is low, selects the amount of scattered light that related coefficient is high.
Herein, for benchmark related coefficient, estimate that low value is also about 0.94.If benchmark related coefficient is decided to be 0.9300, does not then remove the amount of scattered light that photodetector 206 detects yet, and be chosen as the high amount of scattered light of related coefficient.Accuracy class preferably required by its concentration analysis of the value of benchmark related coefficient and suitably setting.
Be judged to be that the data of the angle-data that correlativity is low do not use (step 306) as the data of concentration analysis in step 305.On the contrary, for the data being judged to be the angle-data that correlativity is high, angle-data high for correlativity normalization is treated to the output valve (step 307) of references angle.Normalization process uses is obtaining related coefficient (contribution rate, R as described above
2) time the coefficient (degree of tilt/intercept) of regression straight line that simultaneously calculates, be normalized to the value of the amount of scattered light detected by specific photodetector.
Make the scattered light equalization (step 308) after normalization, finally, carry out the computing (step 309) of concentration analysis according to the amount of scattered light after equalization, until end data process (step 310).
Next, the setting of the parameter of carrying out concentration analysis is described.
Figure 10 represents the setting screen of the parameter of concentration analysis.The setting screen of this parameter shows in CRT monitor (display device) 25.
As mentioned above, for concentration analysis, measure from multi-direction (multi-angle) and obtain the amount of scattered light from determination object, from the data of this amount of scattered light obtained, accepting or rejecting the data selecting there is reliability, and be normalized to the value of the scattered light of the angle of specifying.
Therefore, as shown in Figure 10, from the setting screen of analytical parameters, namely select to be used for making the normalized references angle of scattered light (402) from the selection picture (401) of multiple angle (θ 1, θ 2, θ 3, θ 4).Thereby, it is possible to make the scattered light from other angle standardize relative to the scattered light from arbitrary angle.
In addition, starting point (403) is relatively set as by what start to compare in the interval of comparing correlationship in the data of course of reaction, and be relatively set as end point (404) by what terminate to compare, the setting of concentration analysis can be carried out thus from the analysis condition setting screen of automatic analysing apparatus.The course of reaction etc. of sample preferably can consider the accuracy class required for its concentration analysis, changing according to passage of time, and the setting selecting this starting point (403) and end point (404) is divided with arbitrary passage of time.
In addition, the setting of concentration analysis condition not necessarily must set from the setting screen of automatic analysing apparatus, if fixing condition then also can use the parameter value of the storage area being pre-stored within automatic analysing apparatus.
The explanation of symbol
1-reaction tray, 2-reaction vessel, 3-calibration cell, 4-constant temperature keeping device, 5-sample disc, 6-physical container, 7-moveable arm, 8-pipettor nozzle, 9A, 9B-reagent cold storage box, 12A, 12B-reagent pipettor nozzle, 15-light scattering photometer, 18-computing machine, 19-interface, 204-photodetector (θ 1), 205-photodetector (θ 2), 206-photodetector (θ 3), 207-photodetector (θ 4), 201-light source, 202-reaction vessel, 203-bubble or scar.
Claims (13)
1. an automatic analysing apparatus, it possess to determination object irradiate light thus produce the light source of scattered light, computing machine that multiple photodetector of measuring from the light quantity of multiple mensuration directions to the scattered light from this determination object and the data that obtain the amount of scattered light measured by above-mentioned photodetector go forward side by side row relax, the feature of this automatic analysing apparatus is
Above computer determines the photodetector as benchmark in above-mentioned multiple photodetector, based on the Output rusults of the above-mentioned photodetector as benchmark and the Output rusults of other photodetector, obtain the related coefficient of the mutual above-mentioned amount of scattered light measured in above-mentioned multiple mensuration direction, and the amount of scattered light in the mensuration direction meeting the above-mentioned related coefficient higher than the benchmark related coefficient be pre-entered in above computer is used for the analysis of said determination object.
2. an automatic analysing apparatus, it possess to determination object irradiate light thus produce the light source of scattered light, computing machine that multiple photodetector of measuring from the light quantity of multiple mensuration directions to the scattered light from this determination object changed along with passage of time and the data that obtain the amount of scattered light measured by above-mentioned photodetector go forward side by side row relax, the feature of this automatic analysing apparatus is
Above computer determines the photodetector as benchmark in above-mentioned multiple photodetector, based on the Output rusults of the above-mentioned photodetector as benchmark and the Output rusults of other photodetector, obtain the related coefficient of the mutual above-mentioned amount of scattered light measured in above-mentioned multiple mensuration direction, and the amount of scattered light in the mensuration direction meeting the above-mentioned related coefficient higher than the benchmark related coefficient be pre-entered in above computer is used for the analysis of said determination object.
3. an automatic analysing apparatus, it possess to determination object irradiate light thus produce the light source of scattered light, computing machine that multiple photodetector of measuring the amount of scattered light from determination object from multiple mensuration direction and the data that obtain the amount of scattered light measured by above-mentioned photodetector go forward side by side row relax, the feature of this automatic analysing apparatus is
Above-mentioned photodetector is configured to different relative to the angle of inclination through optical axis through said determination object,
Above computer determines the photodetector as benchmark in above-mentioned multiple photodetector, based on the Output rusults of the above-mentioned photodetector as benchmark and the Output rusults of other photodetector, obtain above-mentioned photodetector mutual detect the related coefficient of amount of scattered light, and the amount of scattered light of the photodetector meeting the above-mentioned related coefficient higher than the benchmark related coefficient be pre-entered in above computer is used for the analysis of said determination object.
4. an automatic analysing apparatus, it possess to determination object irradiate light thus produce the light source of scattered light, computing machine that multiple photodetector of measuring the amount of scattered light from the determination object changed along with passage of time from multiple mensuration direction and the data that obtain the amount of scattered light measured by above-mentioned photodetector go forward side by side row relax, the feature of this automatic analysing apparatus is
Above-mentioned photodetector is configured to different relative to the angle of inclination through optical axis through said determination object,
Above computer determines the photodetector as benchmark in above-mentioned multiple photodetector, based on the Output rusults of the above-mentioned photodetector as benchmark and the Output rusults of other photodetector, obtain above-mentioned photodetector mutual detect the related coefficient of amount of scattered light, and the amount of scattered light of the photodetector meeting the above-mentioned related coefficient higher than the benchmark related coefficient be pre-entered in above computer is used for the analysis of said determination object.
5. the automatic analysing apparatus according to claim 3 or 4, is characterized in that,
The vergence direction of above-mentioned multiple photodetector that angle of inclination is different relative to above-mentionedly becoming up and down through optical axis, left and right or free position in tilting.
6. the automatic analysing apparatus according to claim 3 or 4, is characterized in that,
Above-mentioned multiple photodetector is configured to concentric circles relative to above-mentioned through optical axis.
7. an automatic analysing apparatus, it possess to determination object irradiate light thus produce the light source of scattered light, computing machine that multiple photodetector of measuring the amount of scattered light from determination object from multiple mensuration direction and the data that obtain the amount of scattered light measured by above-mentioned photodetector go forward side by side row relax, the feature of this automatic analysing apparatus is
Above-mentioned photodetector is configured to different relative to the angle of inclination through optical axis through said determination object,
Above computer determines the photodetector as benchmark in above-mentioned multiple photodetector, based on the Output rusults of the above-mentioned photodetector as benchmark and the Output rusults of other photodetector, the related coefficient of the mutual amount of scattered light of each photodetector is obtained according to the regression curve of the amount of scattered light detected
Use except the related coefficient amount of scattered light lower than the benchmark correlation coefficient value be pre-entered in above computer, the above-mentioned amount of scattered light that related coefficient is higher than said reference related coefficient carries out the concentration analysis of said determination object.
8. an automatic analysing apparatus, it possess to determination object irradiate light thus produce the light source of scattered light, computing machine that multiple photodetector of measuring the amount of scattered light from the determination object changed along with passage of time from multiple mensuration direction and the data that obtain the amount of scattered light measured by above-mentioned photodetector go forward side by side row relax, the feature of this automatic analysing apparatus is
Above-mentioned photodetector is configured to different relative to the angle of inclination through optical axis through said determination object,
Above computer determines the photodetector as benchmark in above-mentioned multiple photodetector, based on the Output rusults of the above-mentioned photodetector as benchmark and the Output rusults of other photodetector, the related coefficient of the mutual amount of scattered light of each photodetector is obtained according to the regression curve of the amount of scattered light detected
Use except the related coefficient amount of scattered light lower than the benchmark correlation coefficient value be pre-entered in above computer, the above-mentioned amount of scattered light that related coefficient is higher than said reference related coefficient carries out the concentration analysis of said determination object.
9. the automatic analysing apparatus according to claim 7 or 8, is characterized in that,
The coefficient of the regression straight line calculated when being used in the related coefficient obtaining the above-mentioned related coefficient above-mentioned amount of scattered light higher than said reference related coefficient, carries out normalization process to the value of the amount of scattered light detected by specific photodetector.
10. the automatic analysing apparatus according to claim 2,4 or 8, is characterized in that,
Above-mentioned amount of scattered light can divide with arbitrary passage of time and select above-mentioned amount of scattered light in the scope of determined passage of time.
11. automatic analysing apparatus according to claim 7 or 8, is characterized in that,
When obtaining above-mentioned related coefficient, the regression curve of above-mentioned amount of scattered light can be selected arbitrarily.
12. automatic analysing apparatus according to claim 10, is characterized in that,
Possess the display device of various pictures that display comprises operation screen, setting screen,
Above-mentioned setting screen shows the parameter for carrying out above-mentioned selection.
13. automatic analysing apparatus according to claim 11, is characterized in that,
Possess the display device of various pictures that display comprises operation screen, setting screen,
On above-mentioned setting screen, display is used for carrying out above-mentioned optional parameter.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2010141430A JP5296015B2 (en) | 2010-06-22 | 2010-06-22 | Automatic analyzer |
| JP2010-141430 | 2010-06-22 | ||
| PCT/JP2011/063443 WO2011162113A1 (en) | 2010-06-22 | 2011-06-13 | Automatic analysis device |
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| Publication Number | Publication Date |
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| CN102947690A CN102947690A (en) | 2013-02-27 |
| CN102947690B true CN102947690B (en) | 2015-03-11 |
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| CN201180030416.2A Active CN102947690B (en) | 2010-06-22 | 2011-06-13 | Automatic analysis device |
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| US (1) | US20130108509A1 (en) |
| EP (1) | EP2587250B1 (en) |
| JP (1) | JP5296015B2 (en) |
| CN (1) | CN102947690B (en) |
| WO (1) | WO2011162113A1 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP5822534B2 (en) * | 2011-05-13 | 2015-11-24 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
| JP5897323B2 (en) * | 2011-12-26 | 2016-03-30 | 株式会社日立ハイテクノロジーズ | Automatic analyzer and measurement value abnormality detection method |
| JP6013796B2 (en) * | 2012-06-25 | 2016-10-25 | 株式会社日立ハイテクノロジーズ | Automatic analyzer and sample measurement method |
| JP5984290B2 (en) * | 2012-07-20 | 2016-09-06 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
| JP6138564B2 (en) * | 2013-04-18 | 2017-05-31 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
| JP6134210B2 (en) * | 2013-06-19 | 2017-05-24 | 株式会社日立ハイテクノロジーズ | Automatic analyzer and automatic analysis method |
| JP6567873B2 (en) * | 2015-05-22 | 2019-08-28 | 株式会社日立ハイテクノロジーズ | Automatic analyzer |
| JP2017116351A (en) * | 2015-12-22 | 2017-06-29 | 三菱重工業株式会社 | Evaluation method of bypass foreign matter amount on recirculation sump screen |
| JP6812577B2 (en) | 2017-12-26 | 2021-01-13 | 株式会社日立ハイテク | Automatic analyzer and automatic analysis method |
| WO2024161648A1 (en) * | 2023-02-03 | 2024-08-08 | 日本電信電話株式会社 | Evaluation device, evaluation method, and program |
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| Publication number | Publication date |
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| WO2011162113A1 (en) | 2011-12-29 |
| JP2012007896A (en) | 2012-01-12 |
| CN102947690A (en) | 2013-02-27 |
| EP2587250A1 (en) | 2013-05-01 |
| EP2587250A4 (en) | 2016-06-01 |
| US20130108509A1 (en) | 2013-05-02 |
| JP5296015B2 (en) | 2013-09-25 |
| EP2587250B1 (en) | 2018-03-14 |
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