CN102943057A - Bacillus coagulans and process for high-density fermentation of bacillus coagulans - Google Patents
Bacillus coagulans and process for high-density fermentation of bacillus coagulans Download PDFInfo
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- 238000000855 fermentation Methods 0.000 title claims abstract description 58
- 230000004151 fermentation Effects 0.000 title claims abstract description 58
- 241000193749 Bacillus coagulans Species 0.000 title claims abstract description 30
- 229940054340 bacillus coagulans Drugs 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 28
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 27
- 239000008103 glucose Substances 0.000 claims abstract description 27
- 239000000758 substrate Substances 0.000 claims abstract description 22
- 238000004321 preservation Methods 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 27
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
- 229940041514 candida albicans extract Drugs 0.000 claims description 15
- 239000012138 yeast extract Substances 0.000 claims description 15
- 239000002609 medium Substances 0.000 claims description 13
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 10
- 108010080698 Peptones Proteins 0.000 claims description 10
- 230000001133 acceleration Effects 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 235000019319 peptone Nutrition 0.000 claims description 10
- 230000001580 bacterial effect Effects 0.000 claims description 9
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 7
- 239000013589 supplement Substances 0.000 claims description 7
- 229920001817 Agar Polymers 0.000 claims description 5
- 235000010419 agar Nutrition 0.000 claims description 5
- 239000012092 media component Substances 0.000 claims description 5
- 239000012533 medium component Substances 0.000 claims description 5
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- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 3
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 3
- 235000008434 ginseng Nutrition 0.000 claims description 3
- 239000006916 nutrient agar Substances 0.000 claims description 3
- 230000000050 nutritive effect Effects 0.000 claims description 3
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- 230000001112 coagulating effect Effects 0.000 claims description 2
- 239000006052 feed supplement Substances 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 claims description 2
- 244000131316 Panax pseudoginseng Species 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 239000006041 probiotic Substances 0.000 abstract description 3
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
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Abstract
The invention discloses bacillus coagulans and a process for high-density fermentation of the bacillus coagulans. The Latin name of the strain is Bacillus coagulans, the reference strain is NJYHHWG 877005, the preservation date is October 18, 2012, and the registration number in the preservation center is CGMCC No. 6681. According to the process, the pH value and the glucose concentration are mainly adopted as guides, and the concentrations of different substrates are regulated in different stages to realize feedback, supplementation and culture so as to greatly improve the unit yield and production yield of fermentation. The operational method is convenient and simple and is also conducive to industrial production, and a reference template is provided for mass production and promotion of probiotics.
Description
Technical field
The invention belongs to the fermentation engineering field, relate to the production technique that the fermentation of a kind of Bacillus coagulans and feedback supplement thereof realizes high-density culture.
Background technology
Bacillus coagulans is extensive in distributed in nature, and its gemma has stronger resistivity to heat and other lethal genes, and is also high to the resistance of antibiotic and other pharmaceutical chemicalss.Add the ability that useful Bacillus coagulans has the nutritive substances such as the various proteolytic enzyme of secretion, amylase, lipase, superoxide-dismutase (SOD), antibiotic and multiple amino acids, VITAMIN just because of this characteristic, therefore can be widely used in each field such as medicines and health protection, agricultural and food.
Bacillus coagulans is one of bacterial classification that has in the genus bacillus application potential, and its fungistatic effect is remarkable.This bacterium principal character and ordinary lactic acid bacteria are quite similar, can ferment in large intestine in caecum, colonic field planting after oral, and during the fermentation, can produce short chain fatty acid, such as lactic acid and acetic acid etc.; Bacillus coagulans is facultative anaerobe, can adapt to the intestinal environment of hypoxemia, and entering behind the enteron aisle can consume free oxygen and breeding, is conducive to the growth of anaerobion milk-acid bacteria and bifidus bacillus; This bacterium has high patience to acid and bile, can carry out lactic fermentation, and the L～lactic acid of generation can reduce enteron aisle pH value, suppresses harmful bacteria; Owing to can form gemma, recover the micro ecology of gastrointestinal tract balance so more benefit than the genus bacillus of lactic acid producing not.Thereby promote the intestinal microecology balance, improve immunity of organism and resistance against diseases, the prevention of intestinal tract disease.Bacillus coagulans has very strong restraining effect to the various plants pathogenic bacteria, also can be used as the agricultural produce material and uses.Bacillus coagulans CGMCC No.6681 through the field antibacterial and biological and ecological methods to prevent plant disease, pests, and erosion test also find its to melon, really, the various crop diseases such as the gray molds of the cash crop such as vegetables, banded sclerotial blight, Powdery Mildew, black spot all have significant prevention effect.
In the biotechnology industry platform, the middle and lower reaches technology such as cell large scale cultivation are the weakest sport technique segments of China, the present invention is directed to the bottleneck problem that runs in the Bacillus coagulans industrialization process furthers investigate, and the industrial fermentation technique that cell large scale is cultivated explored, reducing the production cost of Bacillus coagulans, for the scale operation of biological pesticide, promote to provide and to use for reference template.
Summary of the invention
The object of the invention is to improve Bacillus coagulans fermentation unit and production output, for scale operation, the popularization of probiotic bacterium provides a kind of Bacillus coagulans and high cell density fermentation thereof; Mainly be to realize high density fermentation by the feedback supplement fermentation process.
Technical scheme of the present invention is: a kind of genus bacillus, its Classification And Nomenclature is coagulating bacillus strain, the Latin formal name used at school of bacterial classification is: Bacillus coagulans, the bacterial strain of ginseng certificate is: NJYHHWG 877005, preservation date is on October 18th, 2012, and registering on the books and number in the preservation center is CGMCC No.6681.
The present invention also provides a kind of technique of utilizing above-mentioned Bacillus coagulans to carry out high density fermentation, and its concrete steps are as follows: from nutrient agar picking Bacillus coagulans thalline, access seed liquor culture media shaking vase is cultivated to get fermentation seed liquid; Get in the fermentor tank that fermention medium is housed after 4～20% the fermentation seed liquid access sterilization of fermention medium volume and carry out fermentation culture; Concentration by feedback supplement method control nutritive substance is carried out high density fermentation, control temperature, air flow and rotating speed, and fermentation culture 48～78h gets fermented liquid.
Above-mentioned cultivation and fermentation seed liquor is picking thalline from 35～42 ℃ of nutrient agars of cultivating behind 50～72h, access is equipped with in the aseptic Erlenmeyer flask of seed liquor substratum, shaking table is cultivated under 35～42 ℃ of conditions, and control rotating speed 140～200rpm gets fermentation seed liquid behind 32～48h; It is 5～25% of Erlenmeyer flask volume that the seed liquor that wherein fills in the Erlenmeyer flask is cultivated base unit weight.
Preferred described seed liquor nutrient media components is: peptone 8～15g/L, and yeast extract paste 3～8g/L, NaCl4～8g/L, glucose 35～70g/L, pH is controlled at 6.5～7.8.
Preferred fermention medium component: peptone 8～15g/L, yeast extract paste 3～8g/L, NaCl 4～8g/L, glucose 35～70g/L, CaCO
310～15g/L, K
2HPO
40.8～1.5g/L, MgSO
47H
2O 0.1～0.6g/L, ZnSO
47H
2O 0.005～0.015g/L, MnCl
24H
2O 0.001～0.005g/L, pH remains on 6.5～7.8; The fermentor tank liquid amount is 20～45% of fermentor tank volume.
Preferred described feedback supplement adds for carrying out stage by stage feed supplement stream, and fermentation is carried out 16～26h and begun current adding substrate A, and instructs the bottoms stream acceleration with pH value in the fermented liquid, makes the pH value remain on 6.5～7.8; Carry out 12～24h in fermentation and begin, current adding substrate B, and instruct the bottoms stream acceleration with the concentration of glucose makes in the fermentor tank that concentration is controlled at 50～70g/L, fermentation 32～44h in the glucose; Wherein the substrate A composition is: CaCO
355～85g/L, (NH
4)
2HPO
450～80g/L; The substrate B composition is: glucose 200～400g/L, yeast extract paste 50～80g/L, K
2HPO
44.5～8.5g/L, MgSO
47H
2O 0.57～3.5g/L.
The control temperature is at 36～42 ℃ in the preferred fermentation culture process; Pass into sterile air, keep air flow at 3～6L/ (Lmin) with the control dissolved oxygen 30～45%; The control rotating speed is at 140～200rpm.
CGMCC No.6681 bacterial strain has following character:
1, form and cultural characteristic:
This bacterium is G~+ bacteria, and thalline is rod, (0.8～1) μ m * (3～4) μ m, single or one-tenth short chain.Produce the oval gemma, mostly inclined to one side thalline one end bacterium, some sporocysts expand, and mobility is arranged.The environment resistibility is very strong to external world, processes 10min for 90 ℃, and processing 5min for 100 ℃ can inactivation, and the pH value is also can grow in 4.0～9.0 o'clock.Irregular at the nutrient agar upper limb, be the flattened round white colony, 2～3mm size.Facultative anaerobe, growth temperature are 20～55 ℃, and optimum growth temperature is 35～45 ℃.
2, physio-biochemical characteristics
The major physiological biochemical character of Bacillus coagulans CGMCC No.6681 bacterial strain sees Table 1:
The physiological and biochemical property of table 1 bacterial strain
Annotate :+: positive or growth;-: negative or do not grow
Beneficial effect:
The present invention is directed to the bottleneck problem that runs in the course of industrialization and study, a kind of technique of utilizing Bacillus coagulans to carry out high density fermentation is provided.This technique is regulated and control stage by stage the concentration of different substrates and is cultivated to realize feedback supplement mainly take pH value and glucose concn as guidance, and the thalline biomass has been improved about 30%, and viable count reaches 5.8 * 10
9Cfu/mL should invent easy to operate succinct, was conducive to suitability for industrialized production, for the scale operation of probiotic bacterium, promote to provide and can use for reference template.
Preservation information
Its Classification And Nomenclature of above-mentioned Bacillus coagulans is Bacillus coagulans, the microorganism (strain) of ginseng certificate is: NJYHHWG 877005, this bacterial strain is that China Committee for Culture Collection of Microorganisms common micro-organisms center (No. 3, Chaoyang District Beijing Da Tun road first, Institute of Microorganism, Academia Sinica) is independently screened and be preserved in this seminar.It is referred to as CGMCC, and preservation date is on October 18th, 2012, and the numbering of registering on the books is CGMCC No.6681.
Embodiment
The below elaborates to case study on implementation of the present invention, and the implementation case is implemented under take technical solution of the present invention as prerequisite, provided detailed embodiment and the concrete process of serving as, but protection scope of the present invention is not limited to following case study on implementation.
Example 1:
Picking Bacillus coagulans thalline from 35 ℃ of nutrient agars of cultivating behind the 50h, access is equipped with in the aseptic Erlenmeyer flask of seed liquor substratum, and the fermention medium volume accounts for 5% of Erlenmeyer flask volume in the Erlenmeyer flask.The seed liquor nutrient media components is: peptone 8g/L, and yeast extract paste 3g/L, NaCl 4g/L, glucose 35g/L, pH is controlled at 6.5.Shaking table is cultivated under 35 ℃ of conditions, and control rotating speed 140rpm gets fermentation seed liquid behind the 32h.Get in the fermentor tank that fermention medium is housed after the fermentation seed liquid access sterilization of fermention medium volume 4% and carry out fermentation culture.Fermention medium component: peptone 8g/L, yeast extract paste 3g/L, NaCl 4g/L, glucose 35g/L, CaCO
310g/L, K
2HPO
40.8g/L, MgSO
47H
2O 0.1g/L, ZnSO
47H
2O 0.005g/L, MnCl
24H
2O 0.001g/L, pH remains on 6.5, and the fermentor tank liquid amount is 20% of fermentor tank volume.Fermentation is carried out 16h and is begun current adding substrate A, and instructs the bottoms stream acceleration with pH value in the fermented liquid, makes the pH value remain on 6.5; Carry out 12h in fermentation and begin, current adding substrate B, and instruct the bottoms stream acceleration with the concentration of glucose makes in the fermentor tank that concentration is controlled at 50g/L, fermentation 32h in the glucose; Wherein the substrate A composition is: CaCO
355g/L, (NH
4)
2HPO
450g/L; The substrate B composition is: glucose 200g/L, yeast extract paste 50g/L, K
2HPO
44.5g/L, MgSO
47H
2O 0.57g/L.The control temperature is at 36 ℃ in the fermentation culture process; Pass into sterile air, keep air flow at 3L/ (Lmin) with the control dissolved oxygen 30%; The control rotating speed is at 140rpm.Viable count is 8.5 * 10 in the fermented liquid
8Cfu/mL.
Example 2:
Picking Bacillus coagulans thalline from 38 ℃ of nutrient agars of cultivating behind the 61h, access is equipped with in the aseptic Erlenmeyer flask of seed liquor substratum, and the fermention medium volume accounts for 15% of Erlenmeyer flask volume in the Erlenmeyer flask.The seed liquor nutrient media components is: peptone 12g/L, and yeast extract paste 6g/L, NaCl6g/L, glucose 52g/L, pH is controlled at 7.2.Shaking table is cultivated under 38 ℃ of conditions, and control rotating speed 170rpm gets fermentation seed liquid behind the 40h.Get in the fermentor tank that fermention medium is housed after the fermentation seed liquid access sterilization of fermention medium volume 12% and carry out fermentation culture.Fermention medium component: peptone 12g/L, yeast extract paste 6g/L, NaCl 6g/L, glucose 52g/L, CaCO
312g/L, K
2HPO
41.2g/L, MgSO
47H
2O 0.4g/L, ZnSO
47H
2O 0.01g/L, MnCl
24H
2O 0.003g/L, pH remains on 7.2, and the fermentor tank liquid amount is 32% of fermentor tank volume.Fermentation is carried out 21h and is begun current adding substrate A, and instructs the bottoms stream acceleration with pH value in the fermented liquid, makes the pH value remain on 7.2; Carry out 18h in fermentation and begin, current adding substrate B, and instruct the bottoms stream acceleration with the concentration of glucose makes in the fermentor tank that concentration is controlled at 60g/L, fermentation 38h in the glucose; Wherein the substrate A composition is: CaCO
370g/L, (NH
4)
2HPO
465g/L; The substrate B composition is: glucose 300g/L, yeast extract paste 65g/L, K
2HPO
46.5g/L, MgSO
47H
2O 2.0g/L.The control temperature is at 39 ℃ in the fermentation culture process; Pass into sterile air, keep air flow at 4.5L/ (Lmin) with the control dissolved oxygen 37%; The control rotating speed is at 170rpm.Viable count is 5.8 * 10 in the fermented liquid
9Cfu/mL.
Example 3:
Picking Bacillus coagulans thalline from 42 ℃ of nutrient agars of cultivating behind the 72h, access is equipped with in the aseptic Erlenmeyer flask of seed liquor substratum, and the fermention medium volume accounts for 25% of Erlenmeyer flask volume in the Erlenmeyer flask.The seed liquor nutrient media components is: peptone 15g/L, and yeast extract paste 8g/L, NaCl 8g/L, glucose 70g/L, pH is controlled at 7.8.Shaking table is cultivated under 42 ℃ of conditions, and control rotating speed 200rpm gets fermentation seed liquid behind the 48h.Get in the fermentor tank that fermention medium is housed after the fermentation seed liquid access sterilization of fermention medium volume 20% and carry out fermentation culture.Fermention medium component: peptone 15g/L, yeast extract paste 8g/L, NaCl 8g/L, glucose 70g/L, CaCO
315g/L, K
2HPO
41.5g/L, MgSO
47H
2O 0.6g/L, ZnSO
47H
2O0.015g/L, MnCl
24H
2O 0.005g/L, pH remains on 7.8, and the fermentor tank liquid amount is 45% of fermentor tank volume.Fermentation is carried out 26h and is begun current adding substrate A, and instructs the bottoms stream acceleration with pH value in the fermented liquid, makes the pH value remain on 7.8; Carry out 24h in fermentation and begin, current adding substrate B, and instruct the bottoms stream acceleration with the concentration of glucose makes in the fermentor tank that concentration is controlled at 70g/L, fermentation 44h in the glucose; Wherein the substrate A composition is: CaCO
385g/L, (NH
4)
2HPO
480gL; The substrate B composition is: glucose 400g/L, yeast extract paste 80g/L, K
2HPO
48.5g/L, MgSO
47H
2O 3.5g/L.The control temperature is at 42 ℃ in the fermentation culture process; Pass into sterile air, keep air flow at 6L/ (Lmin) with the control dissolved oxygen 45%; The control rotating speed is at 200rpm.Viable count is 3.1 * 10 in the fermented liquid
9Cfu/mL.
Claims (7)
1. genus bacillus, its Classification And Nomenclature is coagulating bacillus strain, and the Latin formal name used at school of bacterial classification is: Bacillus coagulans, and the bacterial strain of ginseng certificate is: NJYHHWG 877005, preservation date is on October 18th, 2012, and registering on the books and number in the preservation center is CGMCC No.6681.
2. technique of utilizing Bacillus coagulans as claimed in claim 1 to carry out high density fermentation, its concrete steps are as follows: from nutrient agar picking Bacillus coagulans thalline, access seed liquor culture media shaking vase is cultivated to get fermentation seed liquid; Get in the fermentor tank that fermention medium is housed after 4～20% the fermentation seed liquid access sterilization of fermention medium volume and carry out fermentation culture; Concentration by feedback supplement method control nutritive substance is carried out high density fermentation, control temperature, air flow and rotating speed, and fermentation culture 48～78h gets fermented liquid.
3. technique according to claim 2, it is characterized in that the cultivation and fermentation seed liquor is picking thalline from 35～42 ℃ of nutrient agars of cultivating behind 50～72h, access is equipped with in the aseptic Erlenmeyer flask of seed liquor substratum, shaking table is cultivated under 35～42 ℃ of conditions, control rotating speed 140～200rpm gets fermentation seed liquid behind 32～48h; It is 5～25% of Erlenmeyer flask volume that the seed liquor that wherein fills in the Erlenmeyer flask is cultivated base unit weight.
4. technique according to claim 2 is characterized in that described seed liquor nutrient media components is: peptone 8～15g/L, and yeast extract paste 3～8g/L, NaCl 4～8g/L, glucose 35～70g/L, pH is controlled at 6.5～7.8.
5. technique according to claim 2 is characterized in that the fermention medium component: peptone 8～15g/L, yeast extract paste 3～8g/L, NaCl 4～8g/L, glucose 35～70g/L, CaCO
310～15g/L, K
2HPO
40.8～1.5g/L, MgSO
47H
2O 0.1～0.6g/L, ZnSO
47H
2O 0.005～0.015g/L, MnCl
24H
2O 0.001～0.005g/L, pH remains on 6.5～7.8; The fermentor tank liquid amount is 20～45% of fermentor tank volume.
6. technique according to claim 2 is characterized in that described feedback supplement adds for carrying out stage by stage feed supplement stream, and fermentation is carried out 16～26h and begun current adding substrate A, and instructs the bottoms stream acceleration with pH value in the fermented liquid, makes the pH value remain on 6.5～7.8; Carry out 12～24h in fermentation and begin, current adding substrate B, and instruct the bottoms stream acceleration with the concentration of glucose makes in the fermentor tank that concentration is controlled at 50～70g/L, fermentation 32～44h in the glucose; Wherein the substrate A composition is: CaCO
355～85g/L, (NH
4)
2HPO
450～80g/L; The substrate B composition is: glucose 200～400g/L, yeast extract paste 50～80g/L, K
2HPO
44.5～8.5g/L, MgSO
47H
2O 0.57～3.5g/L.
7. technique according to claim 2 is characterized in that the control temperature is at 36～42 ℃ in the fermentation culture process; Pass into sterile air, keep air flow at 3～6L/ (Lmin) with the control dissolved oxygen 30～45%; The control rotating speed is at 140～200rpm.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232525A (en) * | 2014-08-29 | 2014-12-24 | 湖北省生物农药工程研究中心 | Process for preparing viable bacillus coagulans preparation |
| CN104738093A (en) * | 2015-03-25 | 2015-07-01 | 南京工业大学 | Preparation method for bacillus coagulans bacterial suspension |
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| CN107568211A (en) * | 2017-08-31 | 2018-01-12 | 南京工业大学 | A kind of method for preparing bacillus coagulans pre-mixing agent |
| CN109609432A (en) * | 2019-01-29 | 2019-04-12 | 北京好实沃生物技术有限公司 | A kind of production spore method of bacillus coagulans |
| CN110272858A (en) * | 2019-07-18 | 2019-09-24 | 江南大学 | A kind of bacillus coagulans of high yield L-lactic acid and its application |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104232525A (en) * | 2014-08-29 | 2014-12-24 | 湖北省生物农药工程研究中心 | Process for preparing viable bacillus coagulans preparation |
| CN104738093A (en) * | 2015-03-25 | 2015-07-01 | 南京工业大学 | Preparation method for bacillus coagulans bacterial suspension |
| WO2016150152A1 (en) * | 2015-03-25 | 2016-09-29 | 南京工业大学 | Preparation method for bacillus coagulans bacterial suspension |
| WO2017012570A1 (en) * | 2015-07-22 | 2017-01-26 | 江南大学 | Bacillus coagulans, high cell density fermentation method thereof, and method for preparing spray-dried powder thereof |
| CN105112326A (en) * | 2015-08-19 | 2015-12-02 | 华南理工大学 | Bacillus and high-density cultivation method for same |
| CN105695304B (en) * | 2016-03-31 | 2018-02-09 | 昆明三正生物科技（集团）有限公司 | A kind of bacillus coagulans fast culture tank |
| CN105695304A (en) * | 2016-03-31 | 2016-06-22 | 昆明三正生物科技（集团）有限公司 | Bacillus coagulans quick culture tank |
| CN106498012A (en) * | 2016-11-18 | 2017-03-15 | 南京工业大学 | One kind is from method for extracting proteins in Bacillus coagulans fed-batch fermentation liquid |
| CN107568211A (en) * | 2017-08-31 | 2018-01-12 | 南京工业大学 | A kind of method for preparing bacillus coagulans pre-mixing agent |
| CN109609432A (en) * | 2019-01-29 | 2019-04-12 | 北京好实沃生物技术有限公司 | A kind of production spore method of bacillus coagulans |
| CN109609432B (en) * | 2019-01-29 | 2022-09-27 | 北京好实沃生物技术有限公司 | Spore production method of bacillus coagulans |
| US20220186234A1 (en) * | 2019-02-20 | 2022-06-16 | Basf Se | Industrial Fermentation Process for Bacillus Using Defined Medium and Trace Element Feed |
| CN110272858A (en) * | 2019-07-18 | 2019-09-24 | 江南大学 | A kind of bacillus coagulans of high yield L-lactic acid and its application |
| CN110272858B (en) * | 2019-07-18 | 2020-09-04 | 江南大学 | Bacillus coagulans for high yield of L-lactic acid and application thereof |
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